Microorganism detection device based on single cell analysis

Abstract

The invention discloses a microbiological detection device based on single cell analysis, which comprises an incubator, an assembly frame is arranged on the upper side of the incubator, a driving part is arranged on the lower side of the assembly frame, the driving end of the driving part penetrates through the assembly frame and is connected with a turntable, and a support frame is arranged on the upper side of the assembly frame. According to the technical scheme, the incubator and the detection probe of the detection device are combined, the incubator and the detection probe of the detection device are assembled in a vertical arrangement mode, the distance between the incubator and the detection probe of the detection device is short, and then detection operation can be directly carried out after microorganism culture is finished; the condition of pollution in the sample transfer process can be reduced, and the detection quality is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of single cell analysis, and in particular to a microorganism detection device based on single cell analysis. Background Art

[0002] Single-cell analysis, as the name suggests, is a multi-dimensional, high-precision test and analysis of individual cells. This analysis method aims to explore the characteristic parameters of each cell, such as the genome, epigenome, transcriptome, gene expression level, protein, target, metabolism, extracellular signal response, etc.

[0003] During the microbial detection process of single-cell analysis, the microbial sample inside the culture device needs to be transferred to the detection device, but sample contamination is prone to occur during the transfer, and the detection device lacks continuity when in use. Only a single culture dish can be detected, and the process of taking and placing the culture dish containing the sample is time-consuming, which in turn leads to reduced efficiency. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a microorganism detection device based on single cell analysis, which has the advantages of high detection efficiency.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A microbial detection device based on single cell analysis, comprising an incubator, characterized in that an assembly frame is provided on the upper side of the incubator, a driving member is provided on the lower side of the assembly frame, a driving end of the driving member passes through the assembly frame and is connected to a turntable, a support frame is provided on the upper side of the assembly frame, and a detection probe is provided on the upper side of the support frame;

[0006] A circle of limit grooves is arranged on the upper side of the turntable near the edge, a rectangular groove connected to the limit groove is arranged on the turntable outside each limit groove, a limit pad is arranged on the inner wall of the limit groove, return springs are arranged on both sides of the rectangular groove, one side of the return spring is connected to the limit block, a connecting seat is arranged on the upper side of the limit block, a transmission frame is connected to the connecting seat, a guide groove is arranged on the outer side of the rectangular groove, the lower side of the transmission frame is slidably connected to the guide groove, a cam is rotatably connected to the outer side of the rectangular groove, a groove corresponding to the cam is opened at the end of the transmission frame, and both sides of the cam extend into the groove.

[0007] In the above solution: The inner part of the incubator is evenly provided with shelves from top to bottom. A lifting plate is arranged on the upper side of each shelf. Both sides of the lifting plate are slidably connected to the inner walls on both sides of the incubator. Electric push rods are arranged on the inner walls on both sides of the incubator. The output end of the electric push rod is connected to the upper side of the lifting plate. The upper side of the shelf is evenly provided with storage grooves. Guide grooves are arranged on the shelves on both sides of the storage groove. A placement box is arranged on the upper side of the storage groove. Both sides of the placement box are slidably connected to the guide grooves through sliders. A culture dish is placed inside the placement box. The upper side of the lifting plate is evenly provided with a sealing component corresponding to the placement groove;

[0008] A box door is rotatably connected to the front side of the incubator. A control device is arranged on one side of the incubator. An observation window is arranged on the front side of the box door.

[0009] In the above solution: The sealing component includes a connecting rod that penetrates through the lower end of the lifting rod. A blocking block is connected to the lower end of the connecting rod. Both sides on the upper side of the blocking block are connected to the lower side of the lifting plate through spring rods. A lifting block is arranged on the upper side of the connecting rod. Arc-shaped grooves are arranged on both sides on the lower side of the lifting block.

[0010] In the above solution: An annular groove is arranged on the upper side of the assembly frame. Universal wheels corresponding to the annular groove are arranged on the lower side of the turntable.

[0011] In the above solution: Through holes are evenly formed on the surfaces of the shelf and the lifting plate. Through holes corresponding to the through holes are formed on the lower side of the placement rack.

[0012] In the above solution: An arc-shaped top block is arranged on one side of the limiting block.

[0013] In the above solution: The diameter of the blocking block is larger than the diameter of the culture dish.

[0014] In the above solution: A handle is arranged on the front side of the placement box.

[0015] In the above solution: The number of the shelves is at least three.

[0016] In the above solution: A knob is connected to the front side of the cam.

[0017] A microbial detection device based on single-cell analysis provided by the present invention has the following beneficial effects: 1. In this technical solution, the incubator and the detection probe of the detection device are combined and assembled in a vertical arrangement. The distance between the two is short. Therefore, after the microbial culture is completed, the detection operation can be directly carried out, which can reduce the contamination during the sample transfer process and improve the detection quality.

[0018] 2. By setting a turntable on the detection probe and adding multiple groups of limit slots on the outer side of the turntable, when detecting microorganisms, multiple culture dishes can be fixed inside the limit slots, thereby increasing the continuity of the detection process and improving the detection efficiency.

[0019] 3. Multiple groups of shelves are arranged inside the incubator, and independent placement boxes are slidably connected to the upper side of the shelves. At the same time, the multiple groups of placement boxes cooperate with the lifting plate and the sealing component to prevent contamination of other culture dishes inside the incubator when the operator opens the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic front sectional view of the main structure of the present invention.

[0021] Figure 2 It is a schematic top view of the turntable of the present invention.

[0022] Figure 3 It is a schematic enlarged view of part A of the present invention.

[0023] Figure 4 It is a schematic enlarged view of part B of the present invention.

[0024] Figure 5 It is a schematic partial front view of the main structure of the present invention.

[0025] Figure 6 It is a schematic top view of the placement box of the present invention.

[0026] In the figure: 1. Incubator, 2. Assembly rack, 3. Driving member, 4. Turntable, 5. Support frame, 6. Detection probe, 7. Limit slot, 8. Limit pad, 9. Return spring, 10. Limit block, 11. Connection seat, 12. Transmission frame, 13. Cam, 14. Shelf, 15. Lifting plate, 16. Electric push rod, 17. Storage groove, 18. Placement box, 19. Slide block, 20. Culture dish, 21. Box door, 22. Control device, 23. Observation window, 24. Connecting rod, 25. Sealing block, 26. Spring rod, 27. Pulling block, 28. Arc-shaped top block, 29. Identification plate, 30. Handle, 31. Knob. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0027] The following describes the present invention in further detail with reference to the drawings and embodiments.

[0028] Embodiment

[0029] Please refer to Figure 1-6, A microbial detection device based on single-cell analysis, including an incubator 1. An assembly frame 2 is arranged on the upper side of the incubator 1. A driving member 3 is arranged on the lower side of the assembly frame 2. The driving end of the driving member 3 penetrates through the assembly frame 2 and is connected to a turntable 4. A support frame 5 is arranged above the assembly frame 2, and a detection probe 6 is arranged on the upper side of the support frame 5. An annular groove is arranged on the upper side of the assembly frame 2, and universal wheels corresponding to the annular groove are arranged on the lower side of the turntable 4 to increase the stability of the turntable 4.

[0030] A circle of limiting grooves 7 corresponding to culture dishes are evenly arranged on the upper side near the edge of the turntable 4. The limiting grooves 7 are circular grooves. On the turntable 4 outside each limiting groove 7, a rectangular groove communicating with the limiting groove 7 is arranged. The rectangular groove and the fiber groove enclose an opening groove. An arc-shaped limiting pad 8 is arranged on the inner wall of the inner side of the limiting groove 7. Reset springs 9 are arranged on both inner walls of the rectangular groove. One end of the reset spring 9 is fixed on the inner wall of the rectangular groove, and the other end is connected to a limiting block 10. The limiting block 10 is in a < shape, and the two limiting blocks 10 on both sides are arranged oppositely. An arc-shaped top block 28 is arranged on the side of the limiting block 10 close to the limiting groove. The arc-shaped top block 28 and the arc-shaped limiting pad 8 together limit the culture dish in the limiting groove 7. A connecting seat 11 is arranged on the upper side of the limiting block 10, and a transmission frame 12 is connected to the connecting seat 11. The transmission frame 12 is an L-shaped frame, and the end of its vertical part is fixed on the connecting seat 11. A guiding groove is arranged corresponding to the transmission frame 12 outside the rectangular groove. The lower side of the horizontal part of the transmission frame 12 is slidably connected to the guiding groove through a slider. A cam 13 is rotatably connected outside the rectangular groove. A groove corresponding to the cam 13 is arranged at the end of the horizontal part of the transmission frame 12, and both sides of the cam 13 extend into the groove. A knob 31 is connected to the front side of the rotating shaft of the cam 13.

[0031] Laminates 14 are evenly arranged in the incubator 1 from top to bottom. The number of laminates 14 is at least three, and the number of electric push rods 16 is twice that of the laminates 14. A lifting plate 15 is correspondingly arranged on the upper side of each laminate 14. Both sides of the lifting plate 15 are slidably connected to the inner walls of both sides of the incubator 1. Electric push rods 16 are arranged on the inner walls of both sides of the incubator 1, and the output ends of the electric push rods 16 are connected to the upper side of the lifting plate 15. Receiving grooves 17 extending forward and backward are evenly arranged on the upper side of the laminate 14. Guiding grooves extending forward and backward are arranged on both sides of the laminate 14 on both sides of the receiving groove 17. A placement box 18 is arranged in the receiving groove 17. Both sides of the placement box 18 extending to the position above the receiving groove 17 are slidably connected to the guiding groove through sliders 19. A culture dish 20 is placed inside the placement box 18. Sealing components corresponding to the placement grooves are evenly arranged on the upper side of the lifting plate 15.

[0032] The sealing assembly includes a connecting rod 24 whose lower end penetrates through the lifting plate. A sealing block 25 is connected to the lower end of the connecting rod 24. Both ends on the upper side of the sealing block 25 are connected to the lower side of the lifting plate 15 through spring rods 26. A lifting block 27 is arranged at the upper end of the connecting rod 24. The connecting rod 24 and the lifting block 27 form a T shape. Arc-shaped grooves are arranged on both sides of the lower side of the lifting block 27. The diameter of the sealing block 25 is larger than the diameter of the culture dish 20. A door 21 is rotatably connected to the front side of the incubator 1. A control device 22 is arranged on one side of the incubator 1. An observation window 23 is arranged on the door 21. A sign 29 is arranged on the front side of each connecting rod 24. Through holes are evenly formed on the surfaces of the shelf 14 and the lifting plate 15. Through holes corresponding to the through holes are formed on the lower side of the placement rack.

[0033] Before the microbial detection, it is necessary to make the morphological characteristics of the microorganisms more obvious through cultivation, which helps to more accurately identify the types of microorganisms. For this purpose, it is necessary to perform a cultivation operation on the microorganisms. When taking out the culture dish 20, the operator can open the door 21. While opening, the electric push rod 16 operates, which can push the lifting plate 15 downward and can make the sealing block 25 block the upper side of the culture dish 20 inside the placement box 18, so as to prevent the samples inside the culture dish 20 from being contaminated after the door 21 is opened.

[0034] A label corresponding to the microorganism is pasted on the front side of the sign 29. The operator can pull up the sealing block 25 by using the connecting rod 24 and the lifting block 27, compress the spring rod 26, and expose the upper side of the culture dish 20. At this time, the sealing block 25 stops squeezing the culture dish 20. The operator can pull out the placement box 18 along the guiding groove by using the handle 30 and take out the culture dish 20 containing the microorganisms, which is convenient for subsequent detection operations. During the taking-out process, the remaining culture dishes 20 are still in a blocked state, which can prevent the remaining culture dishes 20 from being contaminated. After the door 21 is closed, the electric push rod 16 drives the lifting plate 15 to reset. At this time, the remaining culture dishes 20 are exposed again and continue the cultivation operation.

[0035] After the corresponding culture dish 20 is taken out, the operator can sequentially snap multiple culture dishes 20 into the limiting groove 7 from the upper side. During the snapping process, the outer circumference of the culture dish 20 can push the limiting blocks 10 and the transmission frame 12 on both sides away from each other and compress the return spring 9. After the culture dish 20 is completely snapped into the limiting groove 7, the return spring 9 pushes the limiting block 10 to reset, and cooperates with the arc-shaped top block 28 and the limiting pad 8 to fix the culture dish 20. Then the driving member 3 operates, which can drive the turntable 4 to rotate on the upper side of the assembly frame 2. Then, in cooperation with the detection probe 6, the microorganisms inside the culture dish 20 can be detected one by one, which can increase the continuity of the detection process and improve the detection efficiency.

[0036] After the detection is completed, the operator can rotate the cam 13 through the knob 31, so that through the cooperation of the transmission frame 12 and the connecting seat 11, the two limit blocks 10 can be driven to move away from each other again, so that the limit blocks 10 can be separated. At this time, the culture dish 20 inside the limit groove 7 can be taken out for subsequent processing.

[0037] The laminar plate 14 and the lifting plate 15 are evenly provided with through holes, and the lower side of the placement rack is provided with through holes corresponding to the through holes to ensure the air circulation inside the incubator 1 and improve the microorganism culture effect.

[0038] In this technical solution, the incubator and the detection probe of the detection device are combined and assembled in a vertical arrangement. The distance between them is short. Therefore, after the microorganism culture is completed, the detection operation can be directly carried out, which can reduce the contamination during the sample transfer process and improve the detection quality.

[0039] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microbial detection device based on single-cell analysis, comprising an incubator (1), characterized in that, An assembly frame (2) is provided on the upper side of the incubator (1). A driving member (3) is provided on the lower side of the assembly frame (2). The driving end of the driving member (3) penetrates through the assembly frame (2) and is connected to a turntable (4). A support frame (5) is provided on the upper side of the assembly frame (2). A detection probe (6) is provided on the upper side of the support frame (5). A circle of limiting grooves (7) is provided on the upper side of the turntable (4) near the edge. A rectangular groove communicating with the limiting groove (7) is provided on the turntable (4) outside each limiting groove (7). A limiting pad (8) is provided on the inner wall of the limiting groove (7). Reset springs (9) are provided on both sides of the rectangular groove. One side of the reset spring (9) is connected to a limiting block (10). A connecting seat (11) is provided on the upper side of the limiting block (10). A transmission frame (12) is connected to the connecting seat (11). A guiding groove is provided outside the rectangular groove. The lower side of the transmission frame (12) is slidably connected to the guiding groove. A cam (13) is rotatably connected outside the rectangular groove. A groove corresponding to the cam (13) is provided at the end of the transmission frame (12), and both sides of the cam extend into the groove.

2. The microbial detection device based on single-cell analysis according to claim 1, characterized in that: Laminates (14) are evenly provided inside the incubator (1) from top to bottom. A lifting plate (15) is provided on the upper side of each laminate (14). Both sides of the lifting plate (15) are slidably connected to the inner walls of both sides of the incubator (1). Electric push rods (16) are provided on the inner walls of both sides of the incubator (1). The output end of the electric push rod (16) is connected to the upper side of the lifting plate (15). Storage grooves (17) are evenly provided on the upper side of the laminate (14). Guiding grooves are provided on the laminates on both sides of the storage groove (17). A placement box (18) is provided on the upper side of the storage groove (17). Both sides of the placement box (18) are slidably connected to the guiding groove through sliders (19). A culture dish (20) is placed inside the placement box (18). Sealing components corresponding to the placement grooves are evenly provided on the upper side of the lifting plate (15). A box door (21) is rotatably connected to the front side of the incubator (1). A control device (22) is provided on one side of the incubator (1). An observation window (23) is provided on the front side of the box door (21).

3. The microbial detection device based on single-cell analysis according to claim 2, wherein The sealing component includes a connecting rod (24) whose lower end penetrates through the lifting rod. A blocking block (25) is connected to the lower end of the connecting rod (24). Both sides of the upper side of the blocking block (25) are connected to the lower side of the lifting plate (15) through spring rods (26). A lifting block (27) is provided on the upper side of the connecting rod (24). Arc-shaped grooves are provided on both sides of the lower side of the lifting block (27).

4. The microbial detection device based on single-cell analysis according to any one of claims 1-3, characterized in that, An annular groove is provided on the upper side of the assembly frame (2). Universal wheels corresponding to the annular groove are provided on the lower side of the turntable (4).

5. The microbial detection device based on single-cell analysis according to claim 7, characterized in that Through holes are evenly provided on the surfaces of the laminate (14) and the lifting plate (15). Through holes corresponding to the through holes are provided on the lower side of the placement rack.

6. The microbial detection device based on single-cell analysis according to claim 5, characterized in that, An arc-shaped top block (28) is provided on one side of the limiting block (10).

7. The microbial detection device based on single-cell analysis according to claim 6, wherein The diameter of the blocking block (25) is larger than the diameter of the culture dish (20).

8. The microbial detection device based on single-cell analysis according to claim 7, wherein A handle (30) is provided on the front side of the placement box (18).

9. The microbial detection device based on single-cell analysis according to claim 8, characterized in that, The number of the shelves (14) is at least three.

10. The microbial detection device based on single-cell analysis according to claim 9, wherein, A knob (31) is connected to the front side of the cam (13).

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