Detection device for detecting infectious pathogens by using microfluidic chip

Abstract

The invention discloses a detection device for detecting infectious pathogens by using a microfluidic chip, and relates to the technical field of detection.The detection device is characterized in that a disinfection cavity is formed in one side of a detection cavity, a through groove is formed to connect the detection cavity and the disinfection cavity, and a traction mechanism is matched to transversely move the microfluidic chip; the microfluidic chip can move back and forth between the detection cavity and the disinfection cavity, can be cleaned and disinfected in advance before an infectious pathogen sample is detected, and is directly loaded into the detection cavity in a manner of not being in contact with the outside; according to the invention, the influence on the detection result caused by pollution of the microfluidic chip in the manual loading process can be avoided, the detection accuracy can be guaranteed, the microfluidic chip can be automatically transferred into the disinfection cavity for disinfection and cleaning after detection is finished, the probability of contact between an operator and the used microfluidic chip can be effectively reduced, and the detection efficiency is improved. And the detection safety can be ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of detection, and particularly to a detection device for detecting infectious pathogens using a microfluidic chip. Background Art

[0002] In the field of medical detection, the microfluidic chip detection technology has the advantages of miniaturization, integration, fast analysis speed, and small required sample and reagent amounts. This enables detection devices based on microfluidic chips to be widely used in the detection of infectious pathogens.

[0003] Currently, when a detection device based on a microfluidic chip is actually used, it usually requires an operator to manually load the microfluidic chip into the detection equipment. This manual loading process has obvious defects. During the loading process, since the operator needs to directly contact the microfluidic chip, the microfluidic chip is easily contaminated during this process. Once the microfluidic chip is contaminated, foreign contaminants may interfere with the biochemical reactions during subsequent detection processes, resulting in deviations in the detection results and false positive or false negative error results, affecting the accuracy of the determination of infectious pathogen detection results.

[0004] Therefore, a detection device for detecting infectious pathogens using a microfluidic chip is proposed to solve some problems existing in the above-mentioned prior art. Summary of the Invention

[0005] The purpose of the present invention is to solve the drawback in the prior art that during the detection of infectious pathogens, the disinfected and cleaned microfluidic chip is installed in the detection device by manual loading, and if the operation is not careful, it is easy to cause contamination and affect the detection accuracy. A detection device for detecting infectious pathogens using a microfluidic chip is proposed.

[0006] In order to solve the problems existing in the prior art, the present invention adopts the following technical solutions: A detection device for detecting infectious pathogens using a microfluidic chip, comprising a main body. A detection chamber is arranged inside the main body, and a first cover plate is rotatably installed on the detection chamber. A loading groove is fixed inside the detection chamber, and a connector is fixed on the loading groove. A disinfection chamber arranged side by side with the detection chamber is arranged inside the main body, and a second cover plate is rotatably installed on the disinfection chamber. A clamping frame corresponding to the loading groove is installed inside the disinfection chamber. A liquid supply pipe is fixed on the second cover plate, and a plurality of first spray heads communicated with the liquid supply pipe are provided. A drain port is opened at the bottom of the disinfection chamber. An air pump is fixed inside the main body. An air supply pipe is fixed on the second cover plate, and a plurality of second spray heads communicated with the air supply pipe are provided. An electric heating component is arranged inside the air supply pipe. An exhaust pipe is connected inside the disinfection chamber. A through groove communicating between the detection chamber and the disinfection chamber is opened inside the main body, and the through groove is in the same plane as the clamping frame and is located above the loading groove. A sealing plate is vertically slidably installed inside the through groove. A first electric push rod for driving the sealing plate to lift and lower is fixed inside the main body. A traction mechanism is arranged on one side of the detection chamber far away from the disinfection chamber.

[0007] Preferably, one second spray head is inserted into one first spray head, the second spray head is coaxially arranged with the corresponding first spray head, and the second spray head is hidden inside the corresponding first spray head.

[0008] Preferably, a rubber bladder is fixed on one side of the sealing plate close to the disinfection chamber, and argon gas is filled inside the rubber bladder.

[0009] Preferably, the traction mechanism includes a connecting block received inside the end wall of the detection chamber, and the connecting block is in the same plane as the through groove. A second electric push rod for driving the connecting block to move horizontally is fixed inside the main body. A first air suction hole is opened on one side of the connecting block close to the detection chamber.

[0010] Preferably, a lifting platform is installed in the detection chamber in a lifting manner and is arranged below the middle position of the loading groove, and a second air suction hole is opened at the top of the lifting platform.

[0011] Preferably, a first piston chamber is arranged inside the main body and is located below the lifting platform. A first column rod vertically inserted into the first piston chamber is fixed at the bottom of the lifting platform, and a first piston block adapted to the inner size of the first piston chamber is fixed at the bottom end of the first column rod. A second piston chamber is arranged inside the main body and is located below the sealing plate. A second column rod vertically inserted into the second piston chamber is fixed at the bottom of the sealing plate, and a second piston block adapted to the inner size of the second piston chamber is fixed at the bottom end of the second column rod. The bottom of the second piston chamber is communicated with the bottom of the first piston chamber and is filled with oil.

[0012] Preferably, a rotating shaft is rotatably installed inside the disinfection chamber, and the rotating shaft is fixedly connected with the clamping frame. The clamping frame is rotatably installed inside the disinfection chamber through the rotating shaft. A third air suction hole facing the microfluidic chip is opened inside the clamping frame.

[0013] Preferably, a disk is fixed at the end of the rotating shaft, a Hall sensor sleeved outside the disk is installed in the main body of the host, a worm gear is fixed on the rotating shaft, and a worm is meshed outside the worm gear. The worm is fixedly connected to the driving shaft of the servo motor.

[0014] Preferably, a vacuum generator is fixed in the main body of the host, and the air inlet of the vacuum generator is communicated with the air outlet of the air pump. The air outlet of the vacuum generator is communicated with the air supply pipe. A first jack parallel to the second electric push rod is arranged in the main body of the host. A first plug rod slidably inserted into the first jack is fixed on the connecting block, and a channel connecting the first suction hole and the first jack is penetrated in the first plug rod. A second jack parallel to the second suction hole is arranged in the main body of the host. A second plug rod slidably inserted into the second jack is fixed at the bottom of the second suction hole, and a channel connecting the second suction hole and the second jack is penetrated in the second plug rod. A channel communicated with the third suction hole is opened in the rotating shaft. A sleeve movably sleeved outside the rotating shaft is fixed in the disinfection cavity. A through hole communicating the channel in the rotating shaft and the sleeve is opened on the end wall of the rotating shaft. The negative pressure air extraction ports of the vacuum generator are respectively communicated with the first jack, the second jack and the sleeve, and electric control valves are respectively arranged.

[0015] Preferably, a connecting rod is inserted into the drain port, a floating ball is fixed at the top end of the connecting rod, and a limiting block is fixed at the bottom end of the connecting rod.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. In the present invention, a disinfection cavity is arranged on one side of the detection cavity, and a through groove is arranged to connect the detection cavity and the disinfection cavity. With the cooperation of the traction mechanism for the lateral movement of the microfluidic chip, the microfluidic chip can move back and forth between the detection cavity and the disinfection cavity. This enables the device to clean and disinfect the microfluidic chip in advance before detecting the infectious pathogen sample, and directly load it into the detection cavity in a way that does not contact the outside world, which can avoid affecting the detection result due to the contamination of the microfluidic chip during the artificial loading process, is beneficial to ensuring the detection accuracy. Moreover, after the detection is completed, the used microfluidic chip can be automatically transferred to the disinfection cavity for disinfection and cleaning, without manual handling by the operator, which can effectively reduce the probability of the operator contacting the used microfluidic chip and is beneficial to ensuring the detection safety; 2. In the present invention, by hiding the second nozzle in the corresponding first nozzle, when the device sprays disinfectant on the microfluidic chip in the disinfection cavity through the first nozzle, the air pump can be started to jet air flow through the second nozzle. The high-speed air flow can be ejected from the second nozzle hidden in the first nozzle to assist in the diffusion of the disinfectant ejected from the first nozzle, which can effectively improve the effect of the disinfectant acting on the microfluidic chip, and thus is beneficial to improving the high-efficiency and comprehensiveness of the device for cleaning and disinfecting the microfluidic chip; 3. In the present invention, the card holder is rotatably mounted in the disinfection chamber through a rotating shaft, so that when the detection device cleans and disinfects the microfluidic chip, the card holder can be driven to drive the microfluidic chip to continuously rotate, constantly changing the position state of the microfluidic chip, which can effectively improve the comprehensiveness of the microfluidic chip during cleaning and disinfection. At the same time, by opening the third suction hole in the card holder and relying on negative pressure adsorption, the microfluidic chip can be prevented from detaching from the card holder during the rotating disinfection process, ensuring the stability of the device when cleaning and disinfecting the microfluidic chip to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a perspective view of the present invention; Figure 2 is a top view of the present invention; Figure 3 In the present invention Figure 2 is a cross-sectional view taken along line A-A in; Figure 4 In the present invention Figure 2 is a cross-sectional view taken along line B-B in; Figure 5 In the present invention Figure 2 is a cross-sectional view taken along line C-C in; Figure 6 is a front view of the present invention; Figure 7 In the present invention Figure 6 is a cross-sectional view taken along line D-D in; Figure 8 In the present invention Figure 6 is a cross-sectional view taken along line E-E in; Figure 9 In the present invention Figure 6 is a cross-sectional view taken along line F-F in; Figure 10 is an exploded view of the card holder and the microfluidic chip of the present invention; Figure 11 is a perspective view of the first nozzle and the second nozzle of the present invention; Figure 12 is a perspective view of the sealing plate, the first electric push rod and the rubber bladder of the present invention; Figure 13 is a perspective view of the lifting platform and the second suction hole of the present invention; Figure 14 is a perspective view of the connecting block, the second electric push rod and the first suction hole of the present invention; Figure 15 is a perspective view of the air pump and the vacuum generator of the present invention; Figure 16 is a perspective view of the connecting rod, the floating ball and the limit block of the present invention.

[0018] Reference numerals in the figures: 1. Main body; 101. Detection chamber; 102. First cover plate; 103. Loading groove; 104. Connector; 2. Disinfection chamber; 201. Second cover plate; 202. Bracket; 203. Liquid supply pipe; 204. First spray head; 205. Drain port; 206. Air pump; 207. Air supply pipe; 208. Second spray head; 209. Exhaust pipe; 3. Through groove; 301. Sealing plate; 302. First electric push rod; 303. Rubber bladder; 4. Connecting block; 401. Second electric push rod; 402. First suction hole; 403. First jack; 404. First plug rod; 5. Lifting platform; 501. Second suction hole; 502. First piston chamber; 503. First column rod; 504. First piston block; 505. Second piston chamber; 506. Second column rod; 507. Second piston block; 508. Second jack; 509. Second plug rod; 6. Rotating shaft; 601. Servo motor; 602. Disk; 603. Hall sensor; 604. Worm gear; 605. Worm; 606. Third suction hole; 607. Sleeve; 7. Vacuum generator; 8. Connecting rod; 801. Floating ball; 802. Limit block. Specific embodiments

[0019] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. The described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0020] Embodiment: This embodiment provides a detection device for detecting infectious pathogens using a microfluidic chip. Refer to Figures 1 - 16, specifically, it includes a main body 1. A detection chamber 101 is provided inside the main body 1. A first cover plate 102 is rotatably installed on the detection chamber 101. A loading groove 103 is fixed inside the detection chamber 101, and a connector 104 is fixed on the loading groove 103. A disinfection chamber 2 arranged side by side with the detection chamber 101 is provided inside the main body 1. A second cover plate 201 is rotatably installed on the disinfection chamber 2. A clamping frame 202 corresponding to the loading groove 103 is installed inside the disinfection chamber 2. A liquid supply pipe 203 is fixed on the second cover plate 201, and a plurality of first spray heads 204 communicated with the liquid supply pipe 203 are provided. A drain port 205 is opened at the bottom of the disinfection chamber 2. An air pump 206 is fixed inside the main body 1. An air supply pipe 207 is fixed on the second cover plate 201, and a plurality of second spray heads 208 communicated with the air supply pipe 207 are provided. An electric heating component is arranged inside the air supply pipe 207. An exhaust pipe 209 is connected inside the disinfection chamber 2. A through groove 3 communicating between the detection chamber 101 and the disinfection chamber 2 is opened inside the main body 1. The through groove 3 is in the same plane as the clamping frame 202 and is located above the loading groove 103. A sealing plate 301 is vertically slidably installed inside the through groove 3. A first electric push rod 302 for driving the sealing plate 301 to lift and lower is fixed inside the main body 1. A traction mechanism is arranged on one side of the detection chamber 101 far from the disinfection chamber 2. The traction mechanism includes a connecting block 4 received inside the end wall of the detection chamber 101. The connecting block 4 is in the same plane as the through groove 3. A second electric push rod 401 for driving the connecting block 4 to move horizontally is fixed inside the main body 1. A first air suction hole 402 is opened on one side of the connecting block 4 close to the detection chamber 101. A lifting platform 5 is installed in the detection chamber 101 in a lifting manner and is arranged below the middle position of the loading groove 103. A second air suction hole 501 is opened at the top of the lifting platform 5.

[0021] During the use of the detection device, the operator can use the device to perform detection operations on infectious pathogens. Before detection, the second cover plate 201 is turned upwards to open the disinfection chamber 2, and the microfluidic chip is inserted into the clamping frame 202. Then, the second cover plate 201 is turned downwards to close the disinfection chamber 2. The liquid supply pipe 203 is externally connected to a disinfectant supply mechanism, and the disinfectant supply mechanism continuously supplies disinfectant into the liquid supply pipe 203. Then, the special disinfectant for cleaning and disinfecting the microfluidic chip is split from the liquid supply pipe 203 and sprayed out from a plurality of first spray heads 204, acting on the microfluidic chip inserted in the clamping frame 202 to achieve the cleaning and disinfection operation of the microfluidic chip. The sprayed disinfectant will be discharged through the drain port 205 opened at the bottom of the disinfection chamber 2. After the disinfection of the microfluidic chip is completed, the air pump 206 is powered on and started. Through the connection of the air supply pipe 207, the high-speed flowing air flow is sprayed out from the second spray heads 208 and acts on the microfluidic chip to perform the drying operation on the microfluidic chip. During the drying process, the electric heating component connected to the air supply pipe 207 can be powered on and started to increase the temperature of the air flow, thereby effectively improving the drying efficiency of the microfluidic chip.

[0022] After the microfluidic chip is dried, the first electric push rod 302 is powered on and starts to drive the sealing plate 301 to descend in the through groove 3, so that the through groove 3 is in an unobstructed state. Then, the second electric push rod 401 is powered on and starts to drive the connecting block 4 to move leftward. After the connecting block 4 passes through the through groove 3, it enters the disinfection chamber 2 and abuts against the right end of the microfluidic chip inserted in the card holder 202. Then, the air flow suction in the first suction hole 402 starts, and with the negative pressure adsorption force formed by the air flow suction, the microfluidic chip is connected to the connecting block 4. Then, the second electric push rod 401 starts in the reverse direction to pull the microfluidic chip into the detection chamber 101 from the disinfection chamber 2.

[0023] During the lateral movement of the microfluidic chip, the lifting platform 5 is controlled to rise. The lifting platform 5 and the bottom of the microfluidic chip are on the same plane. Through the upward lifting of the lifting platform 5, it can be ensured that the microfluidic chip is stably laterally moved into the detection chamber 101. When the microfluidic chip is above the loading slot 103, the negative pressure adsorption connection to the microfluidic chip at the first suction hole 402 is released, and the connecting block 4 moves back to its original position and is received in the end wall of the connecting block 4. Then, the second suction hole 501 on the lifting platform 5 will perform an air flow suction operation. With the negative pressure adsorption force formed by the air flow suction, the bottom of the microfluidic chip can be firmly connected to the top of the lifting platform 5. The lifting platform 5 is controlled to descend, so that the microfluidic chip is accurately embedded in the loading slot 103, and the upright interface on the microfluidic chip forms a plug connection with the connector 104. During the process of the microfluidic chip being installed in place, the first electric push rod 302 starts in the reverse direction to control the sealing plate 301 to rise in the through groove 3 and block the through groove 3. After that, the operator can turn up the first cover plate 102 to open the detection chamber 101 and drop the sample onto the microfluidic chip to perform the detection operation of infectious pathogens.

[0024] When the detection operation is completed, the sealing plate 301 is controlled to descend again, so that the through groove 3 is opened. Synchronously, the lifting platform 5 rises to lift the used microfluidic chip upward to a state flush with the through groove 3. After being lifted by the lifting platform 5, the interface on the microfluidic chip is separated from the connector 104. Then, the traction mechanism starts to drive the connecting block 4 to translate in the direction of the disinfection chamber 2, and the used microfluidic chip is pushed into the card holder 202 through the through groove 3. Then, the sealing plate 301, the connecting block 4, and the lifting platform 5 return to their original positions, and the through groove 3 is re-sealed. In this state, the device can spray disinfectant through the first nozzle 204 to disinfect and clean the used microfluidic chip, and blow dry it by spraying air through the second nozzle 208 after disinfection. Then, the operator can turn up the second cover plate 201 to open the disinfection chamber 2 and take out the disinfected and cleaned microfluidic chip for storage.

[0025] During the use of the device, a disinfection chamber 2 is arranged on one side of the detection chamber 101, and a through groove 3 is arranged to connect the detection chamber 101 and the disinfection chamber 2. With the cooperation of the traction mechanism for the lateral movement of the microfluidic chip, the microfluidic chip can move back and forth between the detection chamber 101 and the disinfection chamber 2. This enables the device to clean and disinfect the microfluidic chip in advance before detecting an infectious pathogen sample and load it into the detection chamber 101 in a way that does not contact the outside world, which can effectively avoid the microfluidic chip being contaminated during the manual loading process and affecting the detection result, conducive to ensuring the detection accuracy. Moreover, after the detection is completed, the used microfluidic chip can be automatically transferred to the disinfection chamber 2 for disinfection and cleaning, without the need for manual handling by the operator, with convenient operation and being safe and effective.

[0026] In the specific implementation process, as Figure 1 , Figure 3 and Figure 11 shown, a second nozzle 208 is inserted into a first nozzle 204. The second nozzle 208 is coaxially arranged with the corresponding first nozzle 204, and the second nozzle 208 is hidden inside the corresponding first nozzle 204. During the use of the detection device, by hiding the second nozzle 208 inside the corresponding first nozzle 204, when the device sprays disinfectant into the microfluidic chip in the disinfection chamber 2 through the first nozzle 204, the air pump 206 can be started to carry out air flow spraying through the second nozzle 208. The high-speed air flow sprays out through the second nozzle 208 hidden in the first nozzle 204, which can assist in the diffusion of the disinfectant sprayed from the first nozzle 204, and can effectively improve the effect of the disinfectant acting on the microfluidic chip, and thus is conducive to improving the high efficiency and comprehensiveness of the device when cleaning and disinfecting the microfluidic chip.

[0027] In the specific implementation process, as Figure 5 , Figure 9 and Figure 12As shown, a rubber bladder 303 is fixed to one side of the sealing plate 301 close to the disinfection chamber 2, and argon gas is filled in the rubber bladder 303. During the use of the detection device, when the sealing plate 301 seals the through groove 3, the rubber bladder 303 tightly fills the side of the through groove 3 close to the disinfection chamber 2, so that the through groove 3 is completely closed and blocked, which is beneficial to improving the sealing performance when the through groove 3 is closed. When cleaning and disinfecting the microfluidic chip in the disinfection chamber 2, to improve the cleaning and disinfection effect, the electric heating component can be started to heat up the disinfection chamber 2. The argon gas filled in the rubber bladder 303 can block the heat and prevent the heat from being transferred to the detection chamber 101. Synchronously, the argon gas filled in the rubber bladder 303 expands when heated, driving the rubber bladder 303 to expand further outwards, so that the rubber bladder 303 can fill the through groove 3 more tightly, ensuring the sealing performance at the closed position of the through groove 3. The sealing plate 301 is made of heat-insulating material, and the cooperation between the sealing plate 301 and the argon gas in the rubber bladder 303 can further improve the heat-insulating effect at the through groove 3.

[0028] In the specific implementation process, as Figure 4 and Figure 7 shown, a first piston chamber 502 is arranged in the main body 1 below the lifting platform 5. The bottom of the lifting platform 5 is fixed with a first column rod 503 vertically inserted into the first piston chamber 502, and the bottom end of the first column rod 503 is fixed with a first piston block 504 adapted to the inner size of the first piston chamber 502. A second piston chamber 505 is arranged in the main body 1 below the sealing plate 301. The bottom of the sealing plate 301 is fixed with a second column rod 506 vertically inserted into the second piston chamber 505, and the bottom end of the second column rod 506 is fixed with a second piston block 507 adapted to the inner size of the second piston chamber 505. The bottom of the second piston chamber 505 is communicated with the bottom of the first piston chamber 502 and filled with oil.

[0029] During the use of the detection device, in the initial state, the sealing plate 301 is sealed inside the through groove 3, at this time, the second piston block 507 is in the upper position in the second piston chamber 505, the first piston block 504 is in the lower position in the first piston chamber 502, the oil is below the second piston block 507 in the second piston chamber 505, below the first piston block 504 in the first piston chamber 502, and in the connecting pipeline. When the first electric push rod 302 drives the sealing plate 301 to descend and open in the through groove 3, the second column rod 506 will drive the second piston block 507 to move downward. The plug block 507 moves downward in the second piston chamber 505, pushing the oil in the second piston chamber 505 into the first piston chamber 502, propping up the first piston block 504, and driving the lifting platform 5 to rise upward through the connection with the first column rod 503, so as to lift the microfluidic chip. When the sealing plate 301 is controlled to rise upward, the lifting platform 5 is controlled to descend downward. Through the flow of oil, the lifting linkage control of the sealing plate 301 and the lifting platform 5 is realized, which can effectively improve the convenience and flexibility of the device in actual use.

[0030] In the specific implementation process, Figure 1 , Figure 3 , Figure 9 and Figure 10 As shown, a rotating shaft 6 is rotatably installed in the disinfection chamber 2, and the rotating shaft 6 is fixedly connected to the card frame 202. The card frame 202 is rotatably installed in the disinfection chamber 2 through the rotating shaft 6. A servo motor 601 for driving the rotating shaft 6 to rotate is fixed in the main body 1. A third air suction hole 606 facing the microfluidic chip is provided in the card frame 202. During the use of the detection device, when the microfluidic chip is cleaned and disinfected, the servo motor 601 is powered on and started, which will drive the rotating shaft 6 to drive the card frame 202 to rotate, and then the card frame 202 drives the microfluidic chip to rotate in the disinfection chamber 2. By continuously rotating and constantly changing the position state of the microfluidic chip, the comprehensiveness of the microfluidic chip when being cleaned and disinfected can be effectively improved. During the rotation disinfection process, in order to prevent the microfluidic chip from detaching from the card frame 202, the third air suction hole 606 provided in the card frame 202 will be started to perform airflow suction operation. The negative pressure adsorption force formed by airflow suction allows the microfluidic chip to be stably stored in the card frame 202, which to a certain extent ensures the stability of the device when cleaning and disinfecting the microfluidic chip.

[0031] In the specific implementation process, Figure 3 , Figure 9 and Figure 10As shown, a disk 602 is fixed at the end of the rotating shaft 6. A Hall sensor 603 sleeved outside the disk 602 is installed in the main body 1. A worm gear 604 is fixed on the rotating shaft 6, and a worm 605 is meshed outside the worm gear 604. The worm 605 is fixedly connected to the drive shaft of the servo motor 601. During the use of this detection device, through the cooperation of the disk 602 and the Hall sensor 603, the rotation posture of the rotating shaft 6 can be determined, so as to realize the positioning of the posture of the card holder 202. When the microfluidic chip is horizontally translated, the card holder 202 is controlled to be in a horizontal state, and the opening on the right side of the card holder 202 is aligned with the through groove 3, which can ensure the smooth stability of the horizontal translation of the microfluidic chip between the detection chamber 101 and the disinfection chamber 2. When the microfluidic chip needs to be inserted into the card holder 202 or taken out from the card holder 202, through the rotation of the rotating shaft 6 and the cooperative positioning of the disk 602 and the Hall sensor 603, the card holder 202 can be kept in a vertical state. In this state, the opening of the card holder 202 is adjusted to the upper position, and the operator can pick and place the microfluidic chip vertically, making the picking and placing of the microfluidic chip convenient.

[0032] When the servo motor 601 drives the rotating shaft 6 to drive the card holder 202 to rotate, the drive shaft of the servo motor 601 rotates, driving the worm 605 to rotate. Then, with the meshing of the worm 605 and the worm gear 604, the rotating shaft 6 is driven to rotate, providing power for the rotation of the card holder 202. During this process, relying on the self-locking property during the meshing transmission of the worm gear 604 and the worm 605, the card holder 202 is automatically locked after the rotation adjustment, which can effectively ensure the structural stability when the horizontal and vertical postures of the card holder 202 are adjusted.

[0033] In the specific implementation process, such as Figure 4 、 Figure 9 and Figure 15As shown, a vacuum generator 7 is fixed inside the main body 1. The air inlet of the vacuum generator 7 is communicated with the air outlet of the air pump 206, and the air outlet of the vacuum generator 7 is communicated with the air supply pipe 207. A first jack 403 parallel to the second electric push rod 401 is arranged inside the main body 1. A first plug rod 404 slidably inserted into the first jack 403 is fixed on the connecting block 4, and a channel connecting the first suction hole 402 and the first jack 403 runs through the first plug rod 404. A second jack 508 parallel to the second suction hole 501 is arranged inside the main body 1. A second plug rod 509 slidably inserted into the second jack 508 is fixed at the bottom of the second suction hole 501, and a channel connecting the second suction hole 501 and the second jack 508 runs through the second plug rod 509. A channel communicating with the third suction hole 606 is opened inside the rotating shaft 6. A sleeve 607 movably sleeved outside the rotating shaft 6 is fixed inside the disinfection chamber 2. A through hole communicating the channel inside the rotating shaft 6 and the sleeve 607 is opened on the end wall of the rotating shaft 6. The negative pressure air extraction ports of the vacuum generator 7 are respectively communicated with the first jack 403, the second jack 508 and the sleeve 607, and electric control valves are respectively arranged.

[0034] During the use of the detection device, the first plug rod 404 is slidably inserted into the first jack 403, which can ensure the stability of the lateral movement of the connecting block 4. The second plug rod 509 is slidably inserted into the second jack 508, which can ensure the stability of the up-and-down movement of the lifting platform 5. The device connects the vacuum generator 7 to the air outlet of the air pump 206. After the high-speed air flow ejected from the air outlet of the air pump 206 passes through the vacuum generator 7, under the Bernoulli effect, an air flow suction effect will be formed at the negative pressure air extraction port of the vacuum generator 7. The mixed air flow will be ejected from the air outlet of the vacuum generator 7. This air flow can be ejected from the second spray head 208 through the connection of the air supply pipe 207 and acts inside the disinfection chamber 2. The air flow suction at the negative pressure air extraction port of the vacuum generator 7 will act on the first jack 403, the second jack 508 and the sleeve 607 in sequence through the connection of the pipeline, and then, through the connection of the first plug rod 404, the second plug rod 509 and the channel inside the rotating shaft 6, it is implemented at the first suction hole 402, the second suction hole 501 and the third suction hole 606, and is independently controlled by the corresponding electric control valves. By setting the vacuum generator 7, the air flow supply and air flow suction at many positions can all come from the air pump 206, which can effectively improve the stability and energy saving of the device during operation, and the operation is flexible and convenient.

[0035] In the specific implementation process, such as Figure 3 、 Figure 5 and Figure 16As shown, a connecting rod 8 is inserted into the drain port 205, and a floating ball 801 is fixed to the top of the connecting rod 8, and a limiting block 802 is fixed to the bottom of the connecting rod 8. During the use of the detection device, when there is no liquid in the disinfection chamber 2, under the action of gravity, the floating ball 801 will block the top of the drain port 205 to close the drain port 205. In this state, the gas ejected from the second nozzle 208 will not leak out through the drain port 205 and can only be discharged out through the exhaust pipe 209. When there is liquid in the disinfection chamber 2, the connecting rod 8 will float in the liquid, causing the drain port 205 to open, and then the liquid will be discharged out through the drain port 205. In this state, the liquid accumulates at the bottom of the disinfection chamber 2, and the gas ejected from the second nozzle 208 will also not leak out through the drain port 205, thus avoiding the abnormal discharge of waste gas through the drain port 205 and being beneficial to ensuring the safety and stability of the device during use.

[0036] Specifically, the working principle and operation method of the present invention are as follows: The device drives the rotating shaft 6 to drive the card holder 202 to rotate through the servo motor 601, so that the card holder 202 stands upright with the opening facing upward. The operator flips up the second cover plate 201 to open the disinfection chamber 2, inserts the microfluidic chip to be used into the card holder 202. The air pump 206 starts to perform an air suction operation on the third suction hole 606 through the conversion of the vacuum generator 7 to ensure that the microfluidic chip is received in the card holder 202 at a certain temperature. Then, the operator flips down the second cover plate 201 to close the disinfection chamber 2. The disinfectant supply mechanism continuously supplies disinfectant to a plurality of first spray heads 204 through the liquid supply pipe 203. Through the spraying of the disinfectant, the microfluidic chip in the card holder 202 is disinfected and cleaned. Synchronously, the servo motor 601 drives the rotating shaft 6 to drive the card holder 202 to rotate, driving the microfluidic chip to rotate. And the high-speed air flow provided by the air pump 206 is ejected from the second spray head 208 through the connection of the vacuum generator 7 and the air supply pipe 207, strengthening the spraying range and impact force of the disinfectant. With mutual cooperation, the comprehensiveness and efficiency of the disinfection and cleaning of the microfluidic chip are improved. After disinfection, the first spray head 204 stops spraying the disinfectant, and the second spray head 208 continues to start, and the air flow is heated by the electric heating component to realize the efficient drying operation of the microfluidic chip. Then, the card holder 202 drives the microfluidic chip to maintain a horizontal state. The first electric push rod 302 controls the sealing plate 301 to drop in the through groove 3, manipulates the connecting block 4 to move left and right through the second electric push rod 401, cooperates with the negative pressure adsorption connection at the first suction hole 402, and the lifting of the lifting platform 5 to horizontally move the microfluidic chip in the card holder 202 to directly above the loading groove 103. Then, the air suction at the second suction hole 501 connects the lifting platform 5 and the microfluidic chip, and through the lowering of the lifting platform 5, the microfluidic chip is accurately loaded into the loading groove 103. The operator flips up the first cover plate 102 to open the detection chamber 101 and can add the infectious pathogen sample to the microfluidic chip. The operator flips down the first cover plate 102 to close the detection chamber 101 and uses the device to detect the sample. After the detection is completed, the above operations are performed in reverse, and the used microfluidic chip is sent into the card holder 202 in the disinfection chamber 2 for disinfection operation. After the disinfection is completed, the operator takes out the microfluidic chip for storage.

[0037] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

Claims

1. A detection device for detecting infectious pathogens using a microfluidic chip, comprising a main body (1), characterized in that: A detection chamber (101) is provided inside the main body (1), and a first cover plate (102) is rotatably installed on the detection chamber (101). A loading groove (103) is fixed inside the detection chamber (101), and a connector (104) is fixed on the loading groove (103). A disinfection chamber (2) is provided inside the main body (1), and a second cover plate (201) is rotatably installed on the disinfection chamber (2). A clamping frame (202) is installed inside the disinfection chamber (2). A liquid supply pipe (203) and a plurality of first spray heads (204) are fixed on the second cover plate (201). A drain port (205) is opened at the bottom of the disinfection chamber (2). An air pump (206) is fixed inside the main body (1). An air supply pipe (207) and a plurality of second spray heads (208) are fixed on the second cover plate (201). An exhaust pipe (209) is connected inside the disinfection chamber (2). A through groove (3) communicating between the detection chamber (101) and the disinfection chamber (2) is opened inside the main body (1), and the through groove (3) is in the same plane as the clamping frame (202) and is located above the loading groove (103). A sealing plate (301) is vertically slidably installed inside the through groove (3). A first electric push rod (302) for driving the sealing plate (301) to lift and lower is fixed inside the main body (1). A traction mechanism is provided on one side of the detection chamber (101) away from the disinfection chamber (2).

2. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: One of the second spray heads (208) is inserted into one of the first spray heads (204). The second spray head (208) is coaxially arranged with the corresponding first spray head (204), and the second spray head (208) is hidden inside the corresponding first spray head (204).

3. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A rubber bladder (303) is fixed on one side of the sealing plate (301) close to the disinfection chamber (2), and argon is filled inside the rubber bladder (303).

4. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, wherein: The traction mechanism includes a connecting block (4) received inside the end wall of the detection chamber (101), and the connecting block (4) is in the same plane as the through groove (3). A second electric push rod (401) for driving the connecting block (4) to move horizontally is fixed inside the main body (1). A first air suction hole (402) is opened on one side of the connecting block (4) close to the detection chamber (101).

5. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 4, characterized in that: A lifting platform (5) is installed to lift and lower inside the detection chamber (101) and is located below the middle position of the loading groove (103). A second air suction hole (501) is opened at the top of the lifting platform (5).

6. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 5, characterized in that: A first piston chamber (502) is provided in the main body (1) and located below the lifting table (5). A first column rod (503) vertically inserted into the first piston chamber (502) is fixed to the bottom of the lifting table (5). The bottom end of the first column rod (503) is fixed with a first piston block (504) adapted to the inner size of the first piston chamber (502). A second piston chamber (505) is provided in the main body (1) and located below the sealing plate (301). A second column rod (506) vertically inserted into the second piston chamber (505) is fixed to the bottom of the sealing plate (301). The bottom end of the second column rod (506) is fixed with a second piston block (507) adapted to the inner size of the second piston chamber (505). The bottom of the second piston chamber (505) communicates with the bottom of the first piston chamber (502) and is filled with hydraulic oil.

7. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 5, characterized in that: A rotating shaft (6) is rotatably installed in the disinfection chamber (2), and the rotating shaft (6) is fixedly connected to the clamping frame (202). The clamping frame (202) is rotatably installed in the disinfection chamber (2) through the rotating shaft (6). A servo motor (601) for driving the rotating shaft (6) to rotate is fixed in the main body (1). A third suction hole (606) facing the microfluidic chip is formed in the clamping frame (202).

8. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 7, characterized in that: A magnetic disk (602) is fixed at the end of the rotating shaft (6). A Hall sensor (603) sleeved outside the magnetic disk (602) is installed in the main body (1). A worm gear (604) is fixed on the rotating shaft (6), and a worm (605) is engaged with the outside of the worm gear (604). The worm (605) is fixedly connected to the drive shaft of the servo motor (601).

9. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 7, characterized in that: A vacuum generator (7) is fixed inside the main body (1), and the air inlet of the vacuum generator (7) is communicated with the air outlet of an air pump (206). The air outlet of the vacuum generator (7) is communicated with an air supply pipe (207). A first insertion hole (403) parallel to a second electric push rod (401) is arranged inside the main body (1). A first insertion rod (404) which is slidably inserted into the first insertion hole (403) is fixed on the connection block (4), and a channel connecting a first suction hole (402) and the first insertion hole (403) runs through the first insertion rod (404). A second insertion hole (508) parallel to a second suction hole (501) is arranged inside the main body (1). A second insertion rod (509) which is slidably inserted into the second insertion hole (508) is fixed at the bottom of the second suction hole (501), and a channel connecting the second suction hole (501) and the second insertion hole (508) runs through the second insertion rod (509). A channel communicating with a third suction hole (606) is formed inside a rotating shaft (6). A sleeve (607) which is movably sleeved outside the rotating shaft (6) is fixed inside a disinfection chamber (2). A through hole communicating the channel inside the rotating shaft (6) and the sleeve (607) is formed in the end wall of the rotating shaft (6). The negative pressure air extraction ports of the vacuum generator (7) are respectively communicated with the first insertion hole (403), the second insertion hole (508) and the sleeve (607), and electric control valves are respectively arranged.

10. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A connecting rod (8) is inserted into a drain port (205), and a floating ball (801) is fixed at the top end of the connecting rod (8). A limiting block (802) is fixed at the bottom end of the connecting rod (8).

Images