Sealed reagent reaction device capable of realizing secondary injection for biological detection

Abstract

The invention belongs to the technical field of medical detection, and provides a sealed secondary injection reagent reaction device for biological detection, which comprises a placing unit, an injection unit, a vibration unit and a taking unit, and the placing unit, the injection unit, the vibration unit and the taking unit are sequentially communicated and connected to form a continuous closed reaction cavity, sealing covers are arranged at the upper ends of the placing unit and the taking unit; the sliding groove moves in the continuous closed reaction cavity, a plurality of placing grooves are formed in the sliding groove in a sliding mode, and a plurality of supporting springs are arranged at the lower ends of the placing grooves; according to the invention, through the cooperation of the sealing cover and the continuous closed reaction cavity, the whole-process closed operation of secondary injection and reaction is realized, the pollution risk of a medical detection sample in the detection process is avoided, a pure and stable reaction environment is provided for biological detection, and the reliability of a detection result is effectively guaranteed.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical detection, and specifically relates to a sealed and re-injectable reagent reaction device for biological detection. Background Art

[0002] In the process of the development of modern medical testing technology, the rapid progress of medical biological detection technology has put forward higher requirements for detection equipment. The traditional medical biological detection system generally has limitations such as cumbersome processes, long time consumption, and susceptibility to external interference. Especially in the scenarios of point-of-care testing (POCT) and scientific research experiments, the conventional reagent addition method - that is, the operator uses a pipetting device to manually inject reagents into sample test tubes one by one. However, this method not only significantly reduces the detection throughput but also increases the risk of cross-contamination. This single-thread operation mode has become a key bottleneck restricting the improvement of detection efficiency. Specifically, in the process of inducing the biochemical reaction between the analyte and the reagent, the discrete manual operation adopted in the prior art cannot achieve multi-channel parallel processing, seriously affecting the working efficiency of large-scale sample detection;

[0003] In the Chinese patent with the publication number CN114313942A, a fully sealed and re-injectable reagent reaction box for rapid biological detection is mentioned, including a first baffle, the first baffle is fixedly connected to the bottom frame; a support plate, the support plate is fixedly connected to the bottom frame, and the support plate is connected to the first baffle; a second baffle, the second baffle is connected to the support plate, and the second baffle is connected to the first baffle; a sample addition pushing assembly, the bottom frame is provided with a sample addition pushing assembly for placing a plurality of test tubes containing samples; a forward pushing assembly, the bottom frame is provided with a forward pushing assembly for pushing the test tubes on the sample addition pushing assembly to move; a pear-shaped support frame, the pear-shaped support frame is fixedly connected to the top of the support plate; a large syringe, the large syringe is slidably connected to the pear-shaped support frame, and the large syringe is used for injecting reagents into the test tubes on the sample addition pushing assembly; a first return spring, a first return spring is connected between the large syringe and the pear-shaped support frame; a large piston rod, the large piston rod is slidably connected to the large syringe, and the large piston rod is used for extruding the reagent inside the large syringe.

[0004] However, after the large syringe of this device completes one injection, it is necessary to manually place the reagent completed in one injection under the small syringe again for secondary injection. During this process, its sealing performance cannot be guaranteed, increasing the pollution risk of medical detection samples during the detection process and reducing the reliability of the detection results.

[0005] Therefore, those skilled in the art have proposed a sealed and re-injectable reagent reaction device for biological detection to solve the problems raised in the background art. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides a sealed and secondarily injectable reagent reaction device for biological detection to solve the problems in the background art.

[0007] A sealed and secondarily injectable reagent reaction device for biological detection, comprising:

[0008] A placement unit, an injection unit, a vibration unit, and a taking unit. The placement unit, the injection unit, the vibration unit, and the taking unit are sequentially connected in communication to form a continuous sealed reaction cavity. Sealing caps are provided at the upper ends of both the placement unit and the taking unit;

[0009] A sliding groove, which moves inside the continuous sealed reaction cavity. A plurality of placement grooves are slidably arranged inside the sliding groove. A plurality of support springs are provided at the lower ends of the plurality of placement grooves, and the lower ends of the plurality of support springs are connected to the lower end inside the sliding groove. Reaction bottles are arranged inside the placement grooves;

[0010] A moving member, which is installed on one side inside the continuous sealed reaction cavity and is used to drive the placement grooves to move intermittently;

[0011] Two syringes, both of which are slidably installed at the upper end of the injection unit. A piston rod is slidably installed inside the syringe, and a needle is installed at the lower end of the syringe;

[0012] A driving member, which is installed at the upper end of the injection unit and is used to drive the syringe and the piston rod to descend.

[0013] Preferably, the moving member includes a guide groove provided on one side inside the placement unit, the injection unit, the vibration unit, and the taking unit. A moving plate is slidably installed inside the guide groove. One side of the moving plate is connected to one side of the sliding groove. A threaded rod is rotatably installed inside the guide groove, and the threaded rod is intermittently rotated by an intermittent motor. A threaded ring is arranged inside the moving plate, and the threaded ring is threadedly connected to the threaded rod.

[0014] Preferably, the driving member includes a bracket provided at the upper end of the injection unit. A lifting plate is slidably installed at the front end of the bracket. A connecting frame is installed at the front end of the lifting plate. A driven rack is installed on one side inside the connecting frame. A connecting plate is installed at the lower end of the driven rack, and the two ends of the connecting plate are respectively connected to the two piston rods.

[0015] Preferably, a connecting groove is slidably installed on one side inside the connecting frame. A connecting rod is installed at the upper end of the connecting groove, and the connecting rod is slidably connected to the connecting frame. An elastic member is arranged between the connecting groove and the connecting frame. A telescopic member is installed at the upper end of the bracket, and the output end of the telescopic member is connected to the connecting rod. Connecting plates are installed on both sides of the connecting groove, and the two connecting plates are respectively connected to the two syringes.

[0016] Preferably, a driving rack is slidably installed on one side inside the connecting groove, a gear is rotatably installed inside the connecting groove, both the driving rack and the driven rack are engaged with the gear, a connecting frame is installed at the lower end of the connecting groove, a convex rod is rotatably installed inside the connecting frame, a connecting rod is rotatably installed at the rear end of the convex rod, and the upper end of the connecting rod is rotatably installed on the driving rack.

[0017] Preferably, a driving groove is installed at the upper end of the injection unit, and a slope plate is arranged at the lower middle of the driving groove.

[0018] Preferably, it further includes a vibration member, the vibration member includes a rotating shaft rotatably installed on one side of the vibration unit, the rotating shaft is driven to rotate by an intermittent motor, and a cam is installed at the front end of the rotating shaft.

[0019] Preferably, a support plate is installed at the lower end inside the vibration unit, a driving plate is rotatably installed on the support plate, the convex part of the cam drives the driving plate to tilt, a linkage member is installed at the front end of the driving plate, a fixing plate is installed at the lower end inside the vibration unit, the linkage member rotates through the fixing plate, a linkage frame is installed at one end of the linkage member, a driven plate is installed inside the linkage frame, a limiting plate is installed at the lower end inside the vibration unit, one end of the driven plate is rotatably connected to the limiting plate, a telescopic plate is installed at the lower end inside the vibration unit, the other end of the driven plate is rotatably installed on one side of the telescopic plate, and a pressing plate is installed at the upper end of the telescopic plate.

[0020] Preferably, the two syringes are divided into a primary injection syringe and a secondary injection syringe.

[0021] Preferably, reagent adding tubes are communicated with the outer surfaces of the two syringes.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] Through the cooperation of the sealing cover and the continuous closed reaction cavity, the present invention realizes secondary injection and fully enclosed operation throughout the reaction process, avoiding the pollution risk of medical test samples during the detection process, providing a pure and stable reaction environment for biological detection, and effectively ensuring the reliability of the detection results. Secondly, based on the cooperative linkage structure of the double syringes, combined with the timing control of the convex rod and the slope-shaped driving groove, the needle puncture positioning and the piston rod injection are completed in stages during a single downward movement, realizing the physical isolation of the primary injection and the secondary injection and the reagent injection, avoiding reagent cross-contamination. The elastic member and the telescopic member cooperate to realize the automatic reset of the injection assembly. Combined with the intermittent positioning of the moving member, it ensures that the reaction bottle states at each station are updated synchronously, forming a closed-loop control process and reducing the need for manual intervention. Finally, the intermittent motor drives the step-by-step transmission of the threaded rod and the moving plate, cooperating with the station connection of the injection unit and the vibration unit, realizing the continuous operation of the automatic positioning, double-stage injection and vibration treatment of the reaction bottle, significantly improving the detection efficiency, being able to inject different reagents for the primary injection and the secondary injection, meeting the diverse requirements for reagent addition in complex medical detection processes, improving the flexibility and adaptability of the detection, effectively enhancing the efficiency, accuracy and reliability of medical detection, and having high popularization and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a schematic structural diagram of the present invention;

[0025] Figure 2 is a cross-sectional view of the present invention;

[0026] Figure 3 is a partial schematic structural diagram of the present invention;

[0027] Figure 4 is a partial schematic structural diagram of the present invention;

[0028] Figure 5 is a partial schematic structural diagram of the present invention;

[0029] Figure 6 of the present invention Figure 5 partial enlarged view at A in;

[0030] Figure 7 is a schematic structural diagram of the driving member of the present invention from the first perspective;

[0031] Figure 8 is a schematic structural diagram of the driving member of the present invention from the second perspective;

[0032] Figure 9 is a schematic structural diagram of the vibrating member of the present invention.

[0033] In the figure:

[0034] 1. Placement unit; 2. Injection unit; 3. Vibration unit; 4. Retrieving unit; 5. Sliding groove; 6. Support spring; 7. Placement groove; 8. Reaction flask; 9. Moving part; 91. Guide groove; 92. Moving plate; 93. Threaded rod; 10. Syringe; 11. Piston rod; 12. Needle; 13. Driving part; 131. Bracket; 132. Lifting plate; 133. Connecting frame; 134. Telescopic part; 135. Connecting rod; 136. Elastic part; 137. Connecting groove; 138. Connecting plate; 139. Driving rack; 1310. Gear; 1311. Connecting frame; 1312. Convex rod; 1313. Linking rod; 1314. Driving groove; 1315. Driven rack; 1316. Connecting plate; 14. Vibration part; 141. Rotating shaft; 142. Cam; 143. Support plate; 144. Driving plate; 145. Linking part; 146. Fixed plate; 147. Linking frame; 148. Limiting plate; 149. Driven plate; 1410. Telescopic plate; 1411. Bracing plate. Detailed implementation manners

[0035] The following further describes in detail the implementation manners of the present invention in conjunction with the accompanying drawings and embodiments. The following embodiments are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0036] As shown in the attached Figure 1 to the attached Figure 9 shown:

[0037] Embodiment 1: The present invention provides a sealed and secondarily injectable reagent reaction device for biological detection, including:

[0038] A placement unit 1, an injection unit 2, a vibration unit 3 and a retrieving unit 4. The placement unit 1, the injection unit 2, the vibration unit 3 and the retrieving unit 4 are sequentially connected in communication to form a continuous sealed reaction cavity. Sealing covers are provided at the upper ends of both the placement unit 1 and the retrieving unit 4;

[0039] A sliding groove 5, which moves inside the continuous sealed reaction cavity. A plurality of placement grooves 7 are slidably arranged inside the sliding groove 5. A plurality of support springs 6 are provided at the lower ends of the plurality of placement grooves 7. The lower ends of the plurality of support springs 6 are all connected to the lower end inside the sliding groove 5. A reaction flask 8 is arranged inside the placement groove 7;

[0040] A moving part 9, which is installed on one side inside the continuous sealed reaction cavity. The moving part 9 is used to drive the placement groove 7 to move intermittently;

[0041] Two syringes 10, both of which are slidably installed at the upper end of the injection unit 2. A piston rod 11 is slidably installed inside the syringe 10. A needle 12 is installed at the lower end of the syringe 10;

[0042] The driving member 13 is installed at the upper end of the injection unit 2 and is used to drive the syringe barrel 10 and the piston rod 11 to descend.

[0043] The moving member 9 includes a guide groove 91 provided on one side inside the placement unit 1, the injection unit 2, the vibration unit 3, and the picking unit 4. A moving plate 92 is slidably installed inside the guide groove 91. One side of the moving plate 92 is connected to one side of the sliding groove 5. A threaded rod 93 is rotatably installed inside the guide groove 91. The threaded rod 93 is intermittently rotated by an intermittent motor. A threaded ring is provided inside the moving plate 92, and the threaded ring is threadedly connected to the threaded rod 93.

[0044] The driving member 13 includes a bracket 131 provided at the upper end of the injection unit 2. A lifting plate 132 is slidably installed at the front end of the bracket 131. A connecting frame 133 is installed at the front end of the lifting plate 132. A driven rack 1315 is installed on one side inside the connecting frame 133. A connecting plate 1316 is installed at the lower end of the driven rack 1315, and both ends of the connecting plate 1316 are connected to two piston rods 11 respectively.

[0045] A connecting groove 137 is slidably installed on one side inside the connecting frame 133. A connecting rod 135 is installed at the upper end of the connecting groove 137. The connecting rod 135 is slidably connected to the connecting frame 133. An elastic member 136 is provided between the connecting groove 137 and the connecting frame 133. A telescopic member 134 is installed at the upper end of the bracket 131. The output end of the telescopic member 134 is connected to the connecting rod 135. Connecting plates 138 are installed on both sides of the connecting groove 137, and the two connecting plates 138 are respectively connected to the two syringe barrels 10. The telescopic member 134 includes, but is not limited to, an electric push rod.

[0046] A driving rack 139 is slidably installed on one side inside the connecting groove 137. A gear 1310 is rotatably installed inside the connecting groove 137. Both the driving rack 139 and the driven rack 1315 are engaged with the gear 1310. A connecting frame 1311 is installed at the lower end of the connecting groove 137. A convex rod 1312 is rotatably installed inside the connecting frame 1311. A connecting rod 1313 is rotatably installed at the rear end of the convex rod 1312, and the upper end of the connecting rod 1313 is rotatably installed on the driving rack 139.

[0047] A driving groove 1314 is installed at the upper end of the injection unit 2, and a slope plate is provided at the lower middle of the driving groove 1314.

[0048] The two syringe barrels 10 are divided into a primary injection syringe barrel and a secondary injection syringe barrel. The syringe barrel 10 close to the placement unit 1 is for primary reagent injection, and the syringe barrel 10 far from the placement unit 1 is for secondary reagent injection.

[0049] Reagent addition tubes are communicated with the outer surfaces of the two syringe barrels 10, and a small pump can be connected to the reagent addition tubes to supplement the reagent in the syringe barrels 10.

[0050] As can be seen from the above, during the medical detection process, several reaction bottles 8 to be injected with reagents are installed inside several placement grooves 7 through the placement unit 1, and then the continuous sealed reaction cavity is sealed with a sealing cover, so that the injection and medical detection reaction are carried out in a sealed space, effectively preventing the medical detection samples from being contaminated by the outside world and ensuring the accuracy of the detection results.

[0051] The intermittent motor drives the threaded rod 93 to rotate. The threaded rod 93 interacts with the threaded ring inside the moving plate 92, so that the moving plate 92 drives the sliding groove 5 to move intermittently in the guiding groove 91, thereby realizing the intermittent position transformation of the placement groove 7 and the reaction bottle 8 therein. This process realizes the intermittent position transformation of the placement groove 7 and the reaction bottle 8 therein, ensuring that the reaction bottle 8 can pass through each functional unit in turn and complete the corresponding detection process.

[0052] When the reaction bottle 8 moves to the injection unit 2, by starting the telescopic member 134, the connecting rod 135 and the connecting groove 137 are pushed downward, thereby driving the connecting frame 133 to move downward, and then the lifting plate 132 moves downward at the front end of the bracket 131. Thus, under the connection of the connecting plate 138 and the connecting plate 1316, the two syringes 10 and the piston rods 11 are synchronously driven to move downward, so that the two needles 12 are inserted into the two reaction bottles 8. During this process, the convex rod 1312 moves downward in the middle upper part of the driving groove 1314 where there is no slope, and the syringe 10 and the piston rod 11 remain relatively fixed. When the convex rod 1312 moves downward to the upper end of the slope of the driving groove 1314, the needle 12 is inserted into the reaction bottle 8, and the telescopic member 134 stops moving. The connecting groove 137 is fixed at this position, so that the two syringes 10 stop moving downward. At this time, the convex rod 1312 moves downward along the slope of the driving groove 1314, so that the convex rod 1312 rotates and tilts inside the connecting frame 1311, thereby driving the connecting rod 1313 to rotate and tilt upward, thereby pushing the driving rack 139 to move upward inside the connecting groove 137. At this time, the gear 1310 meshes with the driving rack 139 to drive the gear 1310 to rotate, and then meshes with the driven rack 1315, so that the driven rack 1315 drives the connecting frame 133 and the lifting plate 132 to move downward, and the elastic member 136 is compressed. Thus, further with the syringe 10 remaining fixed, the piston rod 11 is further pushed downward, so that the reagents inside the two syringes 10 are injected into the reaction bottle 8. The reaction bottle 8 close to the placement unit 1 is the first reagent injection, and the reaction bottle 8 far from the placement unit 1 is the second reagent injection. After completion, the syringe 10, the piston rod 11 and the needle 12 return to their original positions under the action of the elastic member 136 and the telescopic member 134 to wait for the next injection. The moving member 9 drives the sliding groove 5 to move, so that the reaction bottle 8 moves one position. The reaction bottle 8 that has undergone the second injection enters the vibration unit 3, the reaction bottle 8 that has undergone the first injection enters the lower end of the second injection syringe 10, and the reaction bottle 8 that has not been injected enters the lower end of the first injection syringe 10.

[0053] Through the cooperation of the sealing cover and the continuous closed reaction cavity, secondary injection and full-process closed operation of the reaction are realized, avoiding the pollution risk of medical test samples during the detection process, providing a pure and stable reaction environment for biological detection, and effectively ensuring the reliability of the detection results. Secondly, based on the cooperative linkage structure of the double syringe 10, combined with the timing control of the convex rod 1312 and the slope-shaped drive groove 1314, the needle 12 puncture positioning and the piston rod 11 injection are completed in stages during a single downward movement, realizing the physical isolation of the primary injection and the secondary injection and the reagent injection, avoiding reagent cross-contamination. The elastic member 136 and the telescopic member 134 cooperate to realize the automatic reset of the injection assembly. Combined with the intermittent positioning of the moving member 9, it ensures that the reaction bottles 8 at each station are updated synchronously, forming a closed-loop control process and reducing the need for manual intervention. Finally, the intermittent motor drives the step-by-step transmission of the threaded rod 93 and the moving plate 92, and cooperates with the station connection of the injection unit 2 and the vibration unit 3 to realize the continuous operation of the automatic positioning, double-stage injection and vibration treatment of the reaction bottles 8, significantly improving the detection efficiency. It can inject different reagents for the primary injection and the secondary injection, meet the diverse requirements for reagent addition in complex medical detection processes, improve the flexibility and adaptability of the detection, and effectively improve the efficiency, accuracy and reliability of medical detection, having high popularization and application value.

[0054] Embodiment 2: This is the second embodiment of the present invention, including a vibrating member 14. The vibrating member 14 includes a rotating shaft 141 rotatably installed on one side of the vibration unit 3. The rotating shaft 141 is driven to rotate by an intermittent motor, and a cam 142 is installed at the front end of the rotating shaft 141.

[0055] A support plate 143 is installed at the lower end inside the vibration unit 3. A drive plate 144 is rotatably installed on the support plate 143. The convex part of the cam 142 drives the drive plate 144 to tilt. A linkage member 145 is installed at the front end of the drive plate 144. A fixing plate 146 is installed at the lower end inside the vibration unit 3. The linkage member 145 rotatably passes through the fixing plate 146. A linkage frame 147 is installed at one end of the linkage member 145. A driven plate 149 is installed inside the linkage frame 147. A limiting plate 148 is installed at the lower end inside the vibration unit 3. One end of the driven plate 149 is rotatably connected to the limiting plate 148. A telescopic plate 1410 is installed at the lower end inside the vibration unit 3. The other end of the driven plate 149 is rotatably installed on one side of the telescopic plate 1410. A pressing plate 1411 is installed at the upper end of the telescopic plate 1410.

[0056] As described above, when the reaction flask 8 needs to enter the vibration unit 3 and the preparation injection stage is in progress at this time, the sliding groove 5 stops moving. At this time, the intermittent motor starts, driving the rotation shaft 141 to rotate, and then driving the cam 142 to rotate synchronously. The convex part of the cam 142 contacts the driving plate 144 during rotation. When the convex part of the cam 142 rotates to contact the driving plate 144, it will push the driving plate 144 upward, causing the driving plate 144 to tilt.

[0057] The tilt of the driving plate 144 drives the linkage 145 at its front end to move upward. Since the linkage 145 rotates through the fixed plate 146, the upward movement of the linkage 145 will drive the linkage frame 147 to move upward.

[0058] The upward movement of the linkage frame 147 further drives the driven plate 149 to rotate around its rotation connection point with the limit plate 148. The other end of the driven plate 149 pulls the telescopic plate 1410 upward. The abutting plate 1411 at the upper end of the telescopic plate 1410 pushes the placement groove 7 upward through the hollow part at the lower end of the sliding groove 5, and the support spring 6 is stretched.

[0059] When the cam 142 continues to rotate and its convex part disengages from the driving plate 144, the driving plate 144 resets under its own gravity and the action of the support spring 6, and then drives the linkage 145, the linkage frame 147, the driven plate 149, the telescopic plate 1410, and the abutting plate 1411 to reset in sequence. The acting force of the abutting plate 1411 on the reaction flask 8 disappears, and the reaction flask 8 falls downward under its own gravity and the action of the support spring 6.

[0060] In this way, the intermittent rotation of the cam 142 drives the entire vibrating part 14 to vibrate the reaction flask 8 up and down periodically, so that the reagent and the sample in the reaction flask 8 are fully mixed, improving the efficiency and uniformity of the medical detection reaction and ensuring the accuracy of the detection result.

[0061] In summary, the present invention realizes the intermittent vibration of the reaction flask 8, provides more perfect reaction conditions for medical detection, and further improves the performance and applicability of the detection device.

[0062] The standard parts used in the present invention can all be purchased from the market. The special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machines, parts, and equipment all adopt conventional models in the prior art. Coupled with the circuit connection adopting the conventional connection method in the prior art, it will not be elaborated here. The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0063] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of such features. The meaning of "a plurality" is two or more, unless specifically defined otherwise.

[0064] In the present invention, unless otherwise clearly defined and limited, terms such as "installed", "connected", "connected to", "fixed" and the like should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0065] In the present invention, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0066] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic descriptions of the above terms do not have to be directed to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0067] In the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are involved, and other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0068] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A sealed and secondarily injectable reagent reaction device for biological detection, characterized in that: Comprising: A placing unit (1), an injection unit (2), a vibration unit (3) and a picking unit (4), wherein the placing unit (1), the injection unit (2), the vibration unit (3) and the picking unit (4) are connected in sequence to form a continuous sealed reaction chamber, and sealing covers are provided at the upper ends of both the placing unit (1) and the picking unit (4); A sliding groove (5) which moves inside the continuous sealed reaction chamber, and a number of placement grooves (7) are slidably arranged inside the sliding groove (5). A number of support springs (6) are provided at the lower ends of the placement grooves (7), and the lower ends of the support springs (6) are all connected to the lower end inside the sliding groove (5). A reaction flask (8) is arranged inside the placement groove (7); A moving member (9) which is installed on one side inside the continuous sealed reaction chamber, and the moving member (9) is used to drive the placement groove (7) to move intermittently; Two syringe barrels (10) which are slidably installed at the upper end of the injection unit (2). A piston rod (11) is slidably installed inside the syringe barrel (10), and a needle head (12) is installed at the lower end of the syringe barrel (10); A driving member (13) which is installed at the upper end of the injection unit (2) and is used to drive the syringe barrel (10) and the piston rod (11) to descend.

2. The reagent reaction device for biological detection that is sealed and can be injected twice as described in claim 1, wherein: The moving member (9) includes a guide groove (91) arranged on one side inside the placing unit (1), the injection unit (2), the vibration unit (3) and the picking unit (4). A moving plate (92) is slidably installed inside the guide groove (91), one side of the moving plate (92) is connected to one side of the sliding groove (5). A threaded rod (93) is rotatably installed inside the guide groove (91), and the threaded rod (93) is intermittently rotated by an intermittent motor. A threaded ring is arranged inside the moving plate (92), and the threaded ring is threadedly connected to the threaded rod (93).

3. The reagent reaction device for biological detection which is sealed and can be injected twice as described in claim 1, characterized in that: The driving member (13) includes a bracket (131) arranged at the upper end of the injection unit (2). A lifting plate (132) is slidably installed at the front end of the bracket (131), a connecting frame (133) is installed at the front end of the lifting plate (132). A driven rack (1315) is installed on one side inside the connecting frame (133), a connecting plate (1316) is installed at the lower end of the driven rack (1315), and the two ends of the connecting plate (1316) are respectively connected to the two piston rods (11).

4. The reagent reaction device for biological detection that is sealed and can be injected twice as described in claim 3, characterized in that: A connecting groove (137) is slidably installed on one side inside the connecting frame (133), a connecting rod (135) is installed at the upper end of the connecting groove (137), and the connecting rod (135) is slidably connected to the connecting frame (133). An elastic member (136) is arranged between the connecting groove (137) and the connecting frame (133). A telescopic member (134) is installed at the upper end of the bracket (131), and the output end of the telescopic member (134) is connected to the connecting rod (135). Connecting plates (138) are installed on both sides of the connecting groove (137), and the two connecting plates (138) are respectively connected to the two syringe barrels (10).

5. The reagent reaction device for biological detection that is sealed and can be injected twice as claimed in claim 4, wherein: A drive rack (139) is slidably installed on one side inside the connecting groove (137), a gear (1310) is rotatably installed inside the connecting groove (137), both the drive rack (139) and the driven rack (1315) are engaged with the gear (1310), a connecting frame (1311) is installed at the lower end of the connecting groove (137), a convex rod (1312) is rotatably installed inside the connecting frame (1311), a connecting rod (1313) is rotatably installed at the rear end of the convex rod (1312), and the upper end of the connecting rod (1313) is rotatably installed on the drive rack (139).

6. The reagent reaction device for biological detection, which is sealed and can be injected twice as described in claim 5, is characterized in that: A drive groove (1314) is installed at the upper end of the injection unit (2), and a slope plate is provided at the lower middle of the drive groove (1314).

7. The reagent reaction device for biological detection, which is sealed and can be injected twice as described in claim 1, is characterized in that: It further includes a vibration member (14), the vibration member (14) includes a rotating shaft (141) rotatably installed on one side of the vibration unit (3), the rotating shaft (141) is driven to rotate by an intermittent motor, and a cam (142) is installed at the front end of the rotating shaft (141).

8. The reagent reaction device for biological detection, which is sealed and can be injected twice as claimed in claim 7, is characterized in that: A support plate (143) is installed at the lower end inside the vibration unit (3), a drive plate (144) is rotatably installed on the support plate (143), the convex part of the cam (142) drives the drive plate (144) to incline, a linkage member (145) is installed at the front end of the drive plate (144), a fixing plate (146) is installed at the lower end inside the vibration unit (3), the linkage member (145) rotatably passes through the fixing plate (146), a linkage frame (147) is installed at one end of the linkage member (145), a driven plate (149) is installed inside the linkage frame (147), a limiting plate (148) is installed at the lower end inside the vibration unit (3), one end of the driven plate (149) is rotatably connected to the limiting plate (148), a telescopic plate (1410) is installed at the lower end inside the vibration unit (3), the other end of the driven plate (149) is rotatably installed on one side of the telescopic plate (1410), and a pressing plate (1411) is installed at the upper end of the telescopic plate (1410).

9. The sealed and secondarily injectable reagent reaction device for biological detection according to claim 1, wherein: The two syringes (10) are divided into a primary injection syringe and a secondary injection syringe.

10. The reagent reaction device for biological detection which is sealed and can be injected twice as described in claim 1, characterized in that: Reagent addition tubes are communicated with the outer surfaces of the two syringes (10).

Images