Method for preventing lamb lip mucosa injury by using chrysanthemum straw-containing silage

Abstract

The invention discloses a method for preventing lamb lip mucosa injury by using silage containing chrysanthemum straws, which comprises the following steps: (1) harvesting the chrysanthemum straws; (2) proportioning chrysanthemum straw silage; (3) adding a silage fermentation inoculant; (4) a chrysanthemum straw silage fermentation mode; (5) a silage feeding method; and (6) feeding management of lambs. By making the silage containing the chrysanthemum straw, innovating the mixed fermentation process of the chrysanthemum straw and the corn straw, conducting scientific proportioning and optimizing the conditioning feeding method for lamb lip mucosal lesion, the lamb lip tissue inflammatory response can be effectively controlled, lip mucosal lesion is effectively prevented while lambs are fed with the silage containing the chrysanthemum straw, and the lambs are fed with the silage containing the chrysanthemum straw. The morbidity of lamb oral diseases is reduced, cost is saved, and efficiency is improved.

Description

[0001] The present invention belongs to the technical field of livestock breeding, and particularly relates to a method for preventing the damage of the oral and lip mucosa of lambs by using chrysanthemum straw silage. Background Art

[0002] In recent years, the incidence of oral and lip mucosal damage in lambs has been relatively high and the harm is great. Viruses, bacteria, etc. easily enter the body through the damaged mucosa of lambs, resulting in secondary infection symptoms such as oral ulcers, sheep pox, diarrhea, etc., with high infectivity, which has always been a problem troubling farmers. Oral and lip mucosal damage in lambs is common during the lactation period. Since lambs are weak in constitution and poor in resistance, they are very vulnerable to the threat of diseases. At the same time, lambs begin to practice eating forage, straw and other foods outside milk, and it is inevitable that the oral and lip mucosa will be injured during this period, continuously threatening the health of the flock. After the oral and lip mucosa of newborn lambs is damaged, they have pain and it is difficult to fully ingest breast milk, seriously affecting the growth and development of lambs, and even starving to death. At the same time, it is also easy to cause the occurrence of oral ulcer diseases. Once a sheep with oral ulcers is found, it needs to be isolated and treated in time. Otherwise, the spread is relatively fast and the incidence rate is as high as more than 30%, bringing serious economic losses to farmers.

[0003] With the rapid development of the number and scale of meat sheep breeding in China in recent years, the disease problems related to oral and lip mucosal damage in lambs have become increasingly prominent. It is very troublesome to prevent and treat with conventional iodine glycerin or gentian violet rubbing, combined with ribavirin or dexamethasone injection, penicillin and streptomycin and other drugs, which is time-consuming and laborious. Generally, in the initial stage of inflammation, it is very difficult for farmers to find the mucosal damage of lambs. In most cases, farmers go to treat after the mucosal damage is serious and there are sporadic erythema papules, erosions or pustule symptoms around the mouth, increasing the treatment difficulty and wasting a lot of manpower and material resources. Many studies have confirmed that chrysanthemum stems and leaves contain chemical components similar to chrysanthemums, including volatile oils, flavonoid components, phenolic acid components, polysaccharide components, etc., and have the functions of clearing heat and detoxifying, anti-inflammatory, antioxidant and enhancing the body's immunity. Processing and modulating it into feed has the potential to prevent mucosal damage in lambs. China produces a large amount of chrysanthemum straw every year. Due to a large amount of chrysanthemum straw being abandoned in the fields or burned in place, it causes waste of resources and environmental pollution. The social and economic benefits generated by its feed utilization and high-value utilization cannot be underestimated. The previous research of the team found that feeding after fermenting chrysanthemum straw is better than direct feeding. Adding an appropriate amount of chrysanthemum straw and corn straw and mixing them for silage to make high-quality roughage can significantly improve the antioxidant capacity of meat sheep and reduce the body's inflammatory response. Currently, the conventional silage on the market has no effect on preventing oral and lip mucosal damage in lambs.

[0004] Therefore, prevention is the key. Preventing the oral and lip mucosal damage in lambs has become an urgent problem to be solved in production applications. Currently, finding a method for preventing the oral and lip mucosal damage in lambs by using chrysanthemum straw silage provides technical guidance for reducing the incidence of oral diseases in lambs. Summary of the Invention

[0005] The object of the present invention is to provide a method for preventing oral and lip mucosal damage in lambs by using chrysanthemum straw silage. While feeding the lambs with silage, it can repair the mucosal damage of the oral and lip parts, reduce the incidence of oral diseases in lambs, and save costs and increase efficiency.

[0006] The technical solution adopted by the present invention is as follows: A method for preventing oral and lip mucosal damage in lambs by using chrysanthemum straw silage, comprising the following steps: (1) Harvesting method of chrysanthemum straw: After chrysanthemums are picked, cut the fresh stems and leaves above the ground, and the stubble height is not less than 15 cm; (2) Ratio of chrysanthemum straw silage: Use fresh corn straw after harvesting the grains, and the stubble height is not less than 15 cm. Mix evenly according to the ratio of chrysanthemum straw: corn straw = 1 - 4: 6 - 9; (3) Addition of silage fermentation inoculant: Install an automatic spraying device on the baler, spray while baling, and evenly add the fermentation inoculant. The composition of the fermentation inoculant is Lactobacillus plantarum: Lactobacillus buchneri: Lactobacillus paracasei = 6: 3: 1, ensuring that the inoculant content in the fresh storage material is ≥ 5×10 5 CFU / g; (4) Fermentation method of chrysanthemum straw silage: Use a baler to compact and bale the crushed chrysanthemum straw and corn straw at high density, and the compaction density is not less than 600 kg / m 3 . The bales of grass are promptly wrapped with a wrapper, and the number of layers of stretch film wrapping is controlled at 4 - 6 layers. After 30 days of wrapping fermentation, it can be unpacked and used; (5) Feeding method of chrysanthemum straw silage: Two months before the lambs are born, during the late pregnancy of the ewes, feed the chrysanthemum straw compound silage, and feed 800 g / head of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm every day respectively; After the lambs are born, during the lactation period of the ewes, feed the chrysanthemum straw compound silage, and feed 1000 g / head of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm every day respectively; The lambs are weaned at 60 days old. After weaning, in the first two weeks, feed the weaned lambs with the silage containing chrysanthemum straw at 8:00 am and 17:00 pm every day, gradually increasing from 0 to 200 g / head, and in the next two weeks, feed the weaned lambs with 200 g / head of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm every day. At the same time, mix an appropriate amount of crushed dry rice straw and wheat bran with the concentrate evenly, and then feed; (6) Feeding management of lambs: Disinfect the sheep house once or twice a week, spray disinfectant above the sheep house, and disinfect with 50% Baidusha (glutaraldehyde and decamethonium bromide solution) at a ratio of 1:1500 - 1:3000, clean the drinking water, and keep the house well - ventilated and the environment good.

[0007] The beneficial effects of the present invention: The present invention makes silage containing chrysanthemum straw, innovates the mixed fermentation process of chrysanthemum straw and corn straw, scientifically proportioned, optimizes the conditioning feeding method for the damage of the lamb's oral mucosa, can effectively control the inflammatory reaction of the lamb's oral tissue, repair the damaged tissue, while the lamb is fed silage, repair the damage of the oral mucosa, reduce the incidence of lamb oral diseases, and save costs and increase efficiency. Description of the Drawings

[0008] Figure 1 shows the effects of different chrysanthemum straw silages on the content of serum inflammatory factors in meat sheep; Figure 2 shows the preventive effects of different chrysanthemum straw silages on the damage of lamb oral mucosa; In the figure: Different lowercase letters indicate significant differences ( P <0.05). Detailed Description of the Invention

[0009] The technical solutions of this patent will be further described in detail below in conjunction with the specific embodiments.

[0010] Test Method In a self - breeding and self - raising Hu sheep farm, select a flock of pregnant ewes with similar weights and good health, set up 4 treatment groups. Two months before the lambs are born, that is, in the late pregnancy of the ewes, start feeding different proportions of chrysanthemum straw silage; after the lambs are born, during the lactation period of the ewes, continue to feed different proportions of chrysanthemum straw silage; when the lambs are weaned at 60 days old, in the first two weeks and the last two weeks after weaning, continue to feed different proportions of chrysanthemum straw silage. Control group (CK): Without chrysanthemum straw, 100% pure corn silage straw, mixed with an appropriate amount of crushed dry rice straw, and formulated into a straw - type TMR feed to feed the weaned lambs. Experimental group 1 (Example 1): 10% chrysanthemum straw replaces corn straw silage (10% chrysanthemum), and the others are the same as the control group; Experimental group 2 (Example 2): 20% chrysanthemum straw replaces corn straw silage (20% chrysanthemum), and the others are the same as the control group; Experimental group 3 (Example 3): 40% chrysanthemum straw replaces corn straw silage (40% chrysanthemum), and the others are the same as the control group.

[0011] Control group (CK): Without chrysanthemum straw, 100% pure corn silage straw, mixed with an appropriate amount of crushed dry rice straw and wheat bran, and fully mixed with the concentrate to formulate a straw - type TMR feed to feed the sheep.

[0012] Experimental group: Example

[0013] A method for preventing the damage of lamb oral mucosa by using chrysanthemum straw silage includes the following steps: (1) Harvesting method of chrysanthemum straw: After chrysanthemums are picked, cut the fresh stems and leaves above the ground, and the stubble height is not less than 15 cm; (2) Ratio of chrysanthemum straw silage: Use fresh corn straw after harvesting the seeds, with a stubble height of not less than 15 cm. Mix evenly according to the ratio of chrysanthemum straw: corn straw = 1:9; (3) Addition of silage fermentation inoculant: Install an automatic spraying device on the baler, spray while baling, and evenly add the fermentation inoculant. The composition of the fermentation inoculant is Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus paracasei = 6:3:1, ensuring that the inoculant content in the fresh storage material ≥ 5×10 5 CFU / g; (4) Fermentation method of chrysanthemum straw silage: Use a baler to compact and bale the crushed chrysanthemum straw and corn straw at high density, with a compaction density of not less than 600 kg / m 3 . Immediately wrap the bales with a wrapper, and control the number of layers of stretch film wrapping at 4 - 6 layers; (5) Feeding method of chrysanthemum straw silage: Two months before the lambs are born, during the late pregnancy of the ewes, feed 10% chrysanthemum straw compound silage, and feed 800 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; After the lambs are born, during the lactation period of the ewes, feed chrysanthemum straw compound silage, and feed 1000 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; The lambs are weaned at 60 days old. After weaning, in the first two weeks, feed the weaned lambs 0 - 200 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day, gradually increasing from 0, and in the next two weeks, feed the weaned lambs 200 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day. At the same time, mix an appropriate amount of crushed dry rice straw and wheat bran with the concentrate thoroughly and then feed; (6) Feeding and management of lambs: Disinfect the sheep house 1 - 2 times a week, spray disinfectant above the sheep house, and disinfect with 50% Baidusha (glutaraldehyde and decamethonium bromide solution) at a ratio of 1:1500 - 1:3000, clean the drinking water, and keep the house well - ventilated and in good environment. Example

[0014] A method for preventing oral and lip mucosal damage of lambs with chrysanthemum straw silage, including the following steps: (1) Harvesting method of chrysanthemum straw: After picking the chrysanthemums, cut the fresh above - ground stems and leaves, with a stubble height of not less than 15 cm; (2) Ratio of chrysanthemum straw silage: Use fresh corn straw after harvesting the seeds, with a stubble height of not less than 15 cm. Mix evenly according to the ratio of chrysanthemum straw: corn straw = 2:8; (3) Addition of silage fermentation inoculant: Install an automatic spraying device on the baler, spray while baling, and evenly add the fermentation inoculant. The composition of the fermentation inoculant is Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus paracasei = 6:3:1, ensuring that the inoculant content in the fresh storage material ≥ 5×105 CFU / g; (4) Chrysanthemum straw silage fermentation method: Use a baler to compact and bale the crushed chrysanthemum straw and corn straw at high density, with a compaction density of not less than 600 kg / m 3 . The bales of grass are promptly wrapped with a wrapper, and the number of layers of stretch film wrapping is controlled at 4 to 6 layers; (5) Feeding method of chrysanthemum straw silage: Two months before the lambs are born, during the late pregnancy of the ewes, feed 20% chrysanthemum straw compound silage, and feed 800 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; after the lambs are born, during the lactation period of the ewes, feed chrysanthemum straw compound silage, and feed 1000 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; the lambs are weaned at 60 days old. After weaning, in the first two weeks, feed the weaned lambs with the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively, gradually increasing from 0 to 200 g / sheep, and in the next two weeks, feed the weaned lambs with 200 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively. At the same time, mix an appropriate amount of crushed dry rice straw and wheat bran with the concentrate fully and evenly, and then feed; (6) Feeding management of lambs: Disinfect the meat sheep house once to twice a week, spray disinfect above the meat sheep house, and disinfect with 50% Baidusha (glutaraldehyde and decamethonium bromide solution) at a ratio of 1:1500 to 1:3000, clean the drinking water, and keep the house ventilated and the environment good. Example

[0015] A method for preventing oral and lip mucosal damage of lambs with chrysanthemum straw silage, comprising the following steps: (1) Chrysanthemum straw harvesting method: After the chrysanthemums are picked, harvest the fresh stems and leaves above the ground, with a stubble height of not less than 15 cm; (2) Ratio of chrysanthemum straw silage: Use the fresh corn straw after harvesting the seeds, with a stubble height of not less than 15 cm. Mix evenly according to the ratio of chrysanthemum straw: corn straw = 4:6; (3) Addition of silage fermentation inoculant: Install an automatic spraying device on the baler, spray while baling, and evenly add the fermentation inoculant. The composition of the fermentation inoculant is Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus paracasei = 6:3:1, ensuring that the inoculant content in the fresh stored material ≥ 5×10 5 CFU / g; (4) Chrysanthemum straw silage fermentation method: Use a baler to compact and bale the crushed chrysanthemum straw and corn straw at high density, with a compaction density of not less than 600 kg / m 3 . The bales of grass are promptly wrapped with a wrapper, and the number of layers of stretch film wrapping is controlled at 4 to 6 layers; (5)Feeding method of chrysanthemum straw silage: In the two months before the lambs are born, during the late pregnancy of the ewes, 40% chrysanthemum straw compound silage is fed, and 800 g / sheep of the silage containing chrysanthemum straw is fed at 8:00 am and 17:00 pm respectively every day; after the lambs are born, during the lactation period of the ewes, chrysanthemum straw compound silage is fed, and 1000 g / sheep of the silage containing chrysanthemum straw is fed at 8:00 am and 17:00 pm respectively every day; the lambs are weaned at 60 days old. After weaning, in the first two weeks, the weaned lambs are fed the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day, gradually increasing from 0 to 200 g / sheep, and in the next two weeks, the weaned lambs are fed 200 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day. At the same time, an appropriate amount of crushed dry rice straw and wheat bran are mixed and fed evenly with the concentrate; (6)Feeding and management of lambs: The meat sheep house is disinfected 1-2 times a week. Spray disinfection is carried out above the meat sheep house, and 50% Baidusha (glutaraldehyde and decamethonium bromide solution) is used for disinfection at a ratio of 1:1500-1:3000. Clean drinking water is provided, and good ventilation and environment in the house are maintained.

[0016] The data were initially statistically analyzed by Excel 2010 software, and one-way ANOVA was performed using SPSS 20.0. The Duncan method was used for multiple comparisons between groups. P <0.05 was considered significantly different.

[0017] Test results: From Figure 1 statistical analysis, compared with the control group, the content of the pro-inflammatory factor TNF-α (tumor necrosis factor) in the serum of meat sheep in Example 2 and Example 3 of the chrysanthemum straw silage addition feeding group was significantly reduced ( P <0.05), and the content of the pro-inflammatory factor TNF-α in the serum of meat sheep in Example 1 decreased slightly, but the difference was not significant ( P＞ 0.05); the content of the anti-inflammatory factor IL-10 (interleukin) in the serum of meat sheep in Example 1 and Example 2 of the chrysanthemum straw silage addition feeding group was significantly higher than that of the control group ( P <0.05), and the content of the anti-inflammatory factor IL-10 in the serum of meat sheep in Example 3 was slightly higher than that of the control group, but the difference was not significant ( P＞ 0.05).

[0018] From Figure 2 it can be seen that the oral mucosa of the lambs in the control group (without feeding chrysanthemum straw silage) was severely damaged and obvious scabs appeared. Compared with the control group, the damage of the oral mucosa of the lambs in the chrysanthemum straw silage addition feeding test group was significantly reduced and no scabs appeared. From Figure 1 and Figure 2Comprehensive analysis shows that chrysanthemum straw silage has good anti-inflammatory effects and has great potential for development and application in preventing the oral and lip mucosal damage of lambs.

[0019] The present invention innovates the mixed fermentation process of chrysanthemum straw and corn straw, with scientific proportioning, and optimizes the conditioning feeding method for preventing the oral and lip mucosal damage of lambs. It can effectively control the inflammatory reaction of the oral and lip tissues of lambs, repair the damaged tissues, and has better anti-inflammatory effects compared with simply feeding corn straw, and has better effects on repairing the mucosal damage of the oral and lip parts.

[0020] According to the three key nodes of the gestation period, lactation period, and weaning time of ewes and the incidence law of the oral and lip mucosal damage of lambs, the present invention innovates the mixed storage method of chrysanthemum straw and corn straw and the feeding method of the silage. While reasonably adding the chrysanthemum straw silage for feeding, it can effectively prevent the oral and lip mucosal damage of lambs, reduce the incidence of oral diseases of lambs, and has high innovation. In the past, due to the oral and lip mucosal damage of lambs, it was often necessary to isolate single lambs and apply disinfectants to the oral and lip parts for anti-inflammatory and bacteriostatic purposes, which was troublesome to operate and had high labor costs; after adding chrysanthemum straw silage in the daily feeding process, the cost was reduced and the efficiency was increased significantly. Further, it shows that chrysanthemum straw has great potential for development and application in preventing the oral and lip mucosal damage of weaned lambs, promoting the healthy development of the mutton sheep industry and the feed utilization of chrysanthemum straw waste resources. When implementing specifically, the method for preventing the oral and lip mucosal damage of lambs by chrysanthemum straw silage can be comprehensively determined according to the simplicity of the method operation and cost factors.

[0021] The above description of the embodiments of the present invention is provided, but it does not limit the present invention. For those skilled in the art of the relevant technical field, various modifications can be made to the technical solutions of the above embodiments without departing from the purpose of the present invention.

Claims

1. A method for preventing lamb oral and lip mucosal damage with chrysanthemum straw silage, characterized in that, Including the following steps: (1) Chrysanthemum straw harvesting method: After picking the chrysanthemums, cut the fresh above-ground stems and leaves; (2) Chrysanthemum straw silage ratio: Mix evenly according to the ratio of chrysanthemum straw: corn straw = 1 - 4:6 - 9; (3) Addition of silage fermentation inoculant: Install an automatic spraying device on the baler, spray while baling, and evenly add the fermentation inoculant; (4) Chrysanthemum straw silage fermentation method: Use a baler to compact and bale the crushed chrysanthemum straw and corn straw at high density. The bales of hay are promptly wrapped with a wrapper and can be unpacked and used after 30 days of wrapped fermentation; (5) Feeding method of chrysanthemum straw silage: Two months before the lambs are born, during the late pregnancy of the ewes, feed the chrysanthemum straw compound silage, and feed 800 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; After the lambs are born, during the lactation period of the ewes, feed the chrysanthemum straw compound silage, and feed 1000 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively every day; The lambs are weaned at 60 days old. After weaning, in the first two weeks, feed the weaned lambs with the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively, gradually increasing from 0 to 200 g / sheep. In the following two weeks, feed the weaned lambs with 200 g / sheep of the silage containing chrysanthemum straw at 8:00 am and 17:00 pm respectively. At the same time, mix an appropriate amount of crushed dry rice straw and wheat bran with the concentrate fully and evenly, and then feed; (6) Feeding and management of lambs: Disinfect the meat sheep house 1 - 2 times a week, spray disinfectant above the meat sheep house, clean the drinking water, and keep the house well-ventilated and in good environment.

2. The method for preventing lamb lip and oral mucosa damage by using the chrysanthemum straw silage as claimed in claim 1, wherein The stubble height of the chrysanthemum straw is not less than 15 cm.

3. The method for preventing lamb oral and lip mucosal damage by using the chrysanthemum straw silage according to claim 2, wherein The corn straw is the fresh corn straw after harvesting the kernels, and the stubble height is not less than 15 cm.

4. The method for preventing lip and oral mucosa damage of lambs by using the chrysanthemum straw silage according to claim 3, wherein, The composition of the fermentation inoculant is Lactobacillus plantarum: Lactobacillus buchneri: Lactobacillus paracasei = 6:3:

1.

5. The method for preventing lamb oral and labial mucosa injury by using the chrysanthemum straw silage according to claim 3, characterized in that, The content of the microbial agent in the guaranteed fresh storage material is ≥ 5×10 5 CFU / g.

6. The method for preventing lip and oral mucosa damage of lambs by using the chrysanthemum straw silage according to claim 4, characterized in that, The compacting density of the silage straw is not less than 600 kg / m 3 .

7. The method for preventing lip and oral mucosa damage of lambs by using the chrysanthemum straw silage according to claim 4, wherein The number of layers of stretch film wrapping is controlled at 4 - 6 layers.

8. The method for preventing lamb oral and lip mucosal damage by using the chrysanthemum straw silage according to claim 6, characterized in that, The disinfectant selected is 50% Baidusha (glutaraldehyde and decamethonium bromide solution) for disinfection at a ratio of 1:1500 - 1:3000.

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