Tissue preservation device for pathological analysis of digestive system department

Abstract

The invention relates to the technical field of medical instruments, in particular to a tissue preservation device for pathology analysis of the digestive system department. A cabinet body is arranged on a moving trolley, a cylindrical groove is formed in the middle of a disc-shaped base with the bottom end connected to a top plate of the cabinet body in a sleeving mode, and a plurality of cylindrical cavities are formed in the outer edge of the disc-shaped base; a central hole and a through hole are formed in the disc-shaped shell sleeved at the top end of the disc-shaped seat; the sample cabin is sleeved in the cylindrical cavity; the liquid supplementing tank and the sterile gas tank are arranged in the cabinet body; a gas path input end and a liquid path input end of the gas-liquid distribution part are respectively connected to output ends of the sterile gas tank and the liquid supplementing tank, the liquid nitrogen assembly comprises a tank seat fixed on the bottom surface of the cylindrical groove, a liquid nitrogen tank and a one-way valve arranged at the bottom of the liquid nitrogen tank, and the tank seat is communicated with an inner cavity of the sample cabin; a sealing plate is arranged in a through hole in the outer edge of the disc-shaped shell rotationally sleeved in the disc-shaped shell; the driving part is used for driving the turntable to rotate; a gas-liquid input piece is arranged at the bottom of the sample container, and stationary liquid adopts a new formula. Pathological analysis tissue samples collected by the digestive system department can be safely and effectively stored.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and specifically provides a tissue preservation device for pathological analysis in gastroenterology. Background Art

[0002] Gastroenterology is a clinical department that studies diseases of the esophagus, stomach, small intestine, large intestine, liver, gallbladder, and pancreas. Among them, in pathological analysis of gastroenterology, the preservation quality of tissue samples directly affects the accuracy of pathological diagnosis.

[0003] Traditionally, when preserving tissue samples for pathological analysis in gastroenterology, the tissue samples are often soaked and preserved in a container filled with formalin fixative. The following defects are likely to exist: The tissue is prone to local compression and deformation due to gravity settlement, affecting the accuracy of pathological analysis; and mechanical vibration during transportation may damage the microscopic structure of the tissue, affecting subsequent sectioning and staining.

[0004] Formalin fixative has strong volatility, is harmful to operators, and cannot maintain the viability of tissue cells for a long time. Summary of the Invention

[0005] The purpose of the present invention is to provide a tissue preservation device for pathological analysis in gastroenterology, which is used to solve the problem that it is difficult to safely and effectively preserve the pathological analysis tissue samples collected in gastroenterology in the prior art.

[0006] To achieve the above object, the present invention provides the following technical solution: A tissue preservation device for pathological analysis in the department of gastroenterology, comprising a mobile cart, a cabinet fixed on the mobile cart, and a sample container. The bottom end of the disc-shaped seat is fixedly sleeved in the middle of the top plate of the cabinet. A cylindrical groove is provided in the middle of the top surface of the disc-shaped seat, and a plurality of cylindrical cavities are provided near the outer edge. The bottom of the disc-shaped shell is fixedly sleeved on the top end of the disc-shaped seat. The disc-shaped shell is respectively provided with a central hole and a through hole corresponding to the cylindrical groove and the cylindrical cavity. The sample chamber is fixedly sleeved in the cylindrical cavity. The liquid replenishing tank is arranged in the inner cavity of the cabinet. The sterile gas tank is arranged in the inner cavity of the cabinet. The gas path and liquid path input ends of the gas-liquid distribution part are respectively connected to the output ends of the sterile gas tank and the liquid replenishing tank. The combined structure of the gas path and liquid path output ends of the gas-liquid distribution part is sleeved on the center of the bottom of the sample chamber. The liquid nitrogen assembly includes a tank seat fixed on the bottom surface of the cylindrical groove, a liquid nitrogen tank, and a one-way valve member fixed on the bottom of the liquid nitrogen tank and detachably assembled on the top end of the tank seat. The tank seat is communicated with the inner cavity of the sample chamber through a pipeline. The turntable in a ring structure is rotatably sleeved in the disc-shaped shell. A through hole is provided near the outer edge of the disc-shaped shell, and a sealing plate is detachably sleeved in the through hole. The driving member is used to drive the turntable to rotate. The top cover is detachably fixed at the central hole. The bottom of the sample container is sleeved with a gas-liquid input member whose bottom end is sleeved with the combined structure of the gas path and liquid path output ends of the gas-liquid distribution part. A microporous shell is provided at the top end of the gas-liquid input member. The preservation liquid filled in the sample container and the liquid replenishing tank includes phosphate buffer solution, paraformaldehyde, glutathione, vitamin C, gentamicin, glycerol, and polyvinylpyrrolidone.

[0007] Preferably, the gas-liquid distribution part includes a cylindrical shell, a plurality of infusion tubes whose inner ends are sleeved on the top side wall of the cylindrical shell along the radial direction, a liquid replenishing valve sleeved on the outer ends of the infusion tubes, a ring-shaped shell fixedly sleeved on the bottom of the cylindrical shell, a plurality of gas delivery tubes whose inner ends are sleeved on the side wall of the ring-shaped shell along the radial direction, an air injection valve sleeved on the outer ends of the gas delivery tubes, and a vertical cylinder whose bottom is sleeved with the output ends of the liquid replenishing valve and the air injection valve. The vertical cylinder is sleeved on the center of the bottom of the sample chamber and the top end is sleeved with the bottom end of the gas-liquid input member. The bottom of the cylindrical shell is connected to the output end of the liquid replenishing tank through a pipeline. The bottom of the ring-shaped shell is connected to the output end of the sterile gas tank through a pipeline.

[0008] Preferably, an internally threaded tube is fixedly sleeved in the center of the bottom of the sample chamber. The top end of the vertical cylinder is butted with an externally threaded tube 1 that is threadedly sleeved and matched with the internally threaded tube. A positioning ring is fixed at the position corresponding to the bottom surface of the internally threaded tube at the top end of the vertical cylinder. A locking nut is threadedly sleeved at the position corresponding to the top surface of the internally threaded tube on the externally threaded tube 1.

[0009] Preferably, the sample container includes a cup body sleeved in the inner cavity of the sample chamber and a cup cover detachably covering the top end of the cup body. The gas-liquid input member includes a vertical tube fixedly sleeved at the center of the bottom of the cup body at the top, a pressing ring fixedly sleeved at the top of the vertical tube and having a bottom surface fitting with the bottom surface of the inner cavity of the cup body, a plug body arranged above the middle of the inner cavity of the vertical tube, and a first spring having a bottom end connected to the outer edge of the top surface of the plug body and a top end connected to the inner wall of the top end of the vertical tube. An annular protrusion supporting the bottom of the plug body is provided in the middle of the inner peripheral wall of the vertical tube. An internal thread matching with the external thread tube 1 is provided at the bottom end of the inner peripheral wall of the vertical tube. The microporous housing is fixed to the top end of the vertical tube.

[0010] Preferably, a liquid pump with an input end extending to the bottom of its inner cavity is provided at the top of the liquid replenishing tank. The output end of the liquid pump is connected to the bottom of the cylindrical housing through a pipeline. A liquid adding valve is sleeved at the top end of the side wall of the liquid replenishing tank. An air pump with an input end extending to the inner cavity of the sterile air tank is provided at the top of the sterile air tank. The output end of the air pump is connected to the bottom of the annular housing through a pipeline. An air adding valve is sleeved at the top end of the side wall of the sterile air tank.

[0011] Preferably, side holes are respectively provided radially between the cylindrical groove and the cylindrical cavities around it. An air inlet joint is provided at the position of the side wall of the sample chamber corresponding to the side holes. A nitrogen injection valve is sleeved at the end of the air inlet joint passing through the side holes. The tank base includes a bottom cylinder fixedly fixed to the middle of the bottom surface of the inner cavity of the cylindrical groove, an external thread tube 2 fixedly docked at the top end of the bottom cylinder, a hemispherical housing fixedly docked at the top end of the external thread tube 2, and joints radially sleeved on the side wall of the bottom cylinder and connected to the nitrogen injection valves one by one through pipelines. A plurality of air inlet holes are provided circumferentially at the bottom of the hemispherical housing. The one-way valve member includes a cylindrical housing fixedly sleeved at the center of the bottom of the liquid nitrogen tank and having a bottom end threadedly sleeved with the external thread tube 2 in a matching manner. A plurality of air outlet holes are provided circumferentially on the top side wall of the cylindrical housing located in the inner cavity of the liquid nitrogen tank. A plug plate is slidably sleeved vertically at the top of the inner cavity of the cylindrical housing. A second spring is connected between the top surface of the plug plate and the top surface of the inner cavity of the cylindrical housing.

[0012] Preferably, the turntable further includes an annular plate rotatably sleeved and matched with the inner cavity of the disc-shaped housing and an external gear ring fixed to the top surface of the annular plate. A side housing is fixedly connected to one side of the disc-shaped housing. A shaft hole is provided at the center of the top surface of the side housing. The driving member includes a motor fixed to the top surface of the side housing and having a power output shaft rotatably sleeved and matched with the shaft hole, and a gear fixedly sleeved at the bottom end of the power output shaft of the motor and meshing with the external gear ring.

[0013] Preferably, a flexible mesh cover is fixed to the inner peripheral wall of the sample chamber. The sample container is fixedly sleeved in the flexible mesh cover. A rubber cushion plate is provided between the mobile trolley and the cabinet body.

[0014] Preferably, a temperature sensor is attached to the inner wall of the sample chamber, pressure sensors are respectively installed on the gas delivery pipe and the infusion pipe, and the nitrogen injection valve, the liquid supplement valve and the gas injection valve are all solenoid valves.

[0015] Preferably, a plurality of knob screws are threadedly sleeved on the outer edge of the top cover in the vertical direction. A handle is installed in the middle of the top surface of the top cover. An annular groove matching the outer edge of the top cover is provided on the outer edge of the top end of the central hole, and screw holes matching the bottom ends of the knob screws are provided on the bottom surface of the annular groove.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: In the sample container of the tissue preservation device for pathological analysis in the department of gastroenterology involved in the present invention, a dynamic suspension technology is adopted to preserve the pathological tissues collected in the department of gastroenterology, so as to avoid tissue compression and deformation, and it is especially suitable for easily folded tissues such as the stomach and intestines.

[0017] The tissue preservation device for pathological analysis in the department of gastroenterology involved in the present invention is convenient for preserving a plurality of different pathological tissues, and is convenient for taking and placing, thereby improving the convenience in the actual application process.

[0018] When preserving pathological tissues, the tissue preservation device for pathological analysis in the department of gastroenterology involved in the present invention adopts a preservation solution with a new formula, solves the problems of the toxicity of formalin, tissue damage and environmental protection, and at the same time meets the high-standard requirements of clinical and scientific research. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a three-dimensional structural schematic diagram of the whole of the present invention; Figure 2 is a three-dimensional structural schematic diagram of the disc-shaped seat of the present invention; Figure 3 is a three-dimensional structural schematic diagram of the disc-shaped housing of the present invention; Figure 4 is a three-dimensional structural schematic diagram of the sample chamber of the present invention; Figure 5 is an exploded structural schematic diagram of the sample container of the present invention; Figure 6 is a front view structural schematic diagram of the gas-liquid input member of the present invention; Figure 7 is a three-dimensional structural schematic diagram of the liquid supplement tank of the present invention; Figure 8 is a three-dimensional structural schematic diagram of the sterile gas tank body of the present invention; Figure 9 is a three-dimensional structural schematic diagram of the gas-liquid distribution member of the present invention; Figure 10 is an exploded structural schematic diagram of the liquid nitrogen assembly of the present invention; Figure 11 Schematic perspective view of the can base of the present invention; Figure 12 Exploded perspective view of the turntable of the present invention; Figure 13 Schematic perspective view of the top cover of the present invention; Figure 14 For the present invention Figure 1 Enlarged schematic view of part A in; Figure 15 For the present invention Figure 10 Enlarged schematic view of part B in.

[0020] In the figure: 1 - Mobile trolley; 2 - Cabinet; 3 - Rubber cushion plate; 4 - Disc-shaped seat; 4.1 - Cylindrical cavity; 4.2 - Cylindrical groove; 4.3 - Side hole; 5 - Disc-shaped housing; 5.1 - Through hole; 5.2 - Central hole; 5.3 - Collar; 5.4 - Side housing; 5.5 - Shaft hole; 6 - Sample chamber; 6.1 - Internal thread sleeve; 6.2 - Intake joint; 6.3 - Nitrogen injection valve; 6.4 - Flexible mesh cover; 7 - Sample container; 7.1 - Cup body; 7.2 - Gas-liquid input part; 7.2.1 - Vertical pipe; 7.2.1.1 - Internal thread; 7.2.1.2 - Annular protrusion; 7.2.2 - Compression ring; 7.2.3 - Microporous housing; 7.2.4 - Plug body; 7.2.5 - Spring one; 7.3 - Cup cover; 8 - Liquid replenishing tank; 8.1 - Liquid pump; 8.2 - Liquid adding valve; 9 - Sterile gas tank; 9.1 - Air pump; 9.2 - Gas adding valve; 10 - Gas-liquid distribution part; 10.1 - Cylindrical housing; 10.2 - Infusion tube; 10.3 - Liquid replenishing valve; 10.4 - Annular housing; 10.5 - Gas transmission pipe; 10.6 - Gas injection valve; 10.7 - Vertical cylinder; 10.7.1 - External thread pipe one; 10.7.2 - Positioning ring; 10.7.3 - Locking nut; 11 - Liquid nitrogen assembly; 11.1 - Liquid nitrogen tank; 11.2 - Can base; 11.2.1 - Bottom cylinder; 11.2.2 - External thread pipe two; 11.2.3 - Hemispherical housing; 11.2.4 - Intake hole; 11.2.5 - Connector; 11.3 - Check valve part; 11.3.1 - Cylindrical housing; 11.3.1.1 - Outlet hole; 11.3.2 - Plug plate; 11.3.3 - Spring two; 12 - Turntable; 12.1 - Annular plate; 12.2 - External gear ring; 12.3 - Through hole; 12.4 - Sealing plate; 13 - Driving member; 13.1 - Motor; 13.2 - Gear; 14 - Top cover; 14.1 - Knob screw; 14.2 - Handle. Detailed implementation

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0022] Please refer to Figures 1 - 15 , the present invention provides a technical solution, a tissue preservation device for pathological analysis in the department of digestive medicine, including a mobile trolley 1 and a cabinet 2 fixed on the mobile trolley 1. Among them, universal wheels with a braking mechanism are installed at the bottom of the mobile trolley 1, and a telescopic push frame is installed at the rear end of the mobile trolley 1 to facilitate the transportation of pathological tissues. A rubber cushion plate 3 is provided between the mobile trolley 1 and the cabinet 2 to relieve the vibration generated by the cabinet 2 when the mobile trolley 1 moves.

[0023] The bottom end of the disc-shaped seat 4 is fixedly sleeved in the middle of the top plate of the cabinet 2. A cylindrical groove 4.2 is provided in the middle of the top surface of the disc-shaped seat 4, and a plurality of cylindrical cavities 4.1 are provided near the outer edge. Among them, the plurality of cylindrical cavities 4.1 are evenly circumferentially arranged around the periphery of the cylindrical groove 4.2, and side holes 4.3 are respectively provided radially between the cylindrical groove 4.2 and the cylindrical cavities 4.1 around it. The disc-shaped seat 4 is integrally processed from a heat-insulating material.

[0024] The bottom end of the disc-shaped housing 5 is fixedly sleeved on the top end of the disc-shaped seat 4. The disc-shaped housing 5 is respectively provided with a central hole 5.2 and a through hole 5.1 corresponding to the cylindrical groove 4.2 and the cylindrical cavity 4.1. Among them, a collar 5.3 integrally formed with the top end of the disc-shaped seat 4 is fixedly sleeved at the bottom end of the disc-shaped housing 5. A side housing 5.4 is connected to one side of the disc-shaped housing 5, and a shaft hole 5.5 is provided at the center of the top surface of the side housing 5.4. An annular groove is provided at the outer edge of the top end of the central hole 5.2, and a plurality of screw holes are provided along the circumferential direction on the bottom surface of the annular groove.

[0025] The sample chamber 6 is fixedly sleeved in the cylindrical cavity 4.1. A female threaded pipe 6.1 is fixedly sleeved at the center of the bottom of the sample chamber 6. An air inlet joint 6.2 is provided on the side wall of the sample chamber 6 corresponding to the position of the side hole 4.3. A nitrogen injection valve 6.3 is sleeved at the end of the air inlet joint 6.2 passing through the side hole 4.3; a flexible mesh cover 6.4 is fixed on the inner peripheral wall of the sample chamber 6.

[0026] The sample container 7 includes a cup body 7.1 sleeved in the inner cavity of a flexible mesh cover 6.4 and a cup lid 7.3 detachably covered on the top end of the cup body 7.1. A gas-liquid input member 7.2 is sleeved at the bottom of the cup body 7.1. Among them, the gas-liquid input member 7.2 includes a vertical tube 7.2.1 fixedly sleeved at the center of the bottom of the cup body 7.1 at the top, a microporous housing 7.2.3 fixedly connected to the top end of the vertical tube 7.2.1, a pressing ring 7.2.2 fixedly sleeved at the top end of the vertical tube 7.2.1 and whose bottom surface fits with the bottom surface of the inner cavity of the cup body 7.1, a plug body 7.2.4 arranged above the middle part of the inner cavity of the vertical tube 7.2.1, and a first spring 7.2.5 whose bottom end is connected to the outer edge of the top surface of the plug body 7.2.4 and whose top end is connected to the inner wall of the top end of the vertical tube 7.2.1. An annular protrusion 7.2.1.2 supporting the bottom of the plug body 7.2.4 is arranged in the middle part of the inner peripheral wall of the vertical tube 7.2.1, and an internal thread 7.2.1.1 is arranged at the bottom end of the inner peripheral wall of the vertical tube 7.2.1. The microporous housing 7.2.3 is a hemispherical housing structure covered with micropores.

[0027] The replenishing liquid tank 8 and the sterile gas tank 9 are both arranged in the inner cavity of the cabinet body 2. A liquid pump 8.1 with an input end extending to the bottom of its inner cavity is arranged at the top of the replenishing liquid tank 8, a liquid adding valve 8.2 is sleeved at the top end of the side wall of the replenishing liquid tank 8, an air pump 9.1 with an input end extending to the inner cavity of the sterile gas tank 9 is arranged at the top of the sterile gas tank 9, and an air adding valve 9.2 is sleeved at the top end of the side wall of the sterile gas tank 9. That is, it is convenient to supplement the preservation liquid into the replenishing liquid tank 8 through the liquid adding valve 8.2; it is convenient to add sterile air into the sterile gas tank 9 through the air adding valve 9.2.

[0028] The gas-liquid distribution member 10 includes a cylindrical housing 10.1, a plurality of liquid infusion tubes 10.2 whose inner ends are radially sleeved on the top side wall of the cylindrical housing 10.1, a liquid replenishing valve 10.3 sleeved at the outer ends of the liquid infusion tubes 10.2, an annular housing 10.4 fixedly sleeved at the bottom of the cylindrical housing 10.1, a plurality of gas delivery tubes 10.5 whose inner ends are radially sleeved on the side wall of the annular housing 10.4, an air injection valve 10.6 sleeved at the outer ends of the gas delivery tubes 10.5, and a vertical cylinder 10.7 whose bottom is sleeved with the output ends of the liquid replenishing valve 10.3 and the air injection valve 10.6. Among them, the output end of the liquid pump 8.1 is connected to the bottom of the cylindrical housing 10.1 through a pipeline, and the output end of the air pump 9.1 is connected to the bottom of the annular housing 10.4 through a pipeline. The top end of the vertical cylinder 10.7 is butted with an external thread tube 10.7.1 that is threadedly sleeved and matched with the internal thread tube 6.1. A positioning ring 10.7.2 is fixed at the position of the top end of the vertical cylinder 10.7 corresponding to the bottom surface of the internal thread tube 6.1, and a locking nut 10.7.3 is threadedly sleeved at the position of the external thread tube 10.7.1 corresponding to the top surface of the internal thread tube 6.1. That is, through the clamping action of the positioning ring 10.7.2 and the locking nut 10.7.3, the vertical cylinder 10.7 is assembled with the corresponding sample chamber 6. The top end of the external thread tube 10.7.1 is threadedly sleeved and matched with the internal thread 7.2..1.1.

[0029] The liquid nitrogen assembly 11 includes a tank base 11.2 fixed to the bottom surface of the cylindrical groove 4.2, a liquid nitrogen tank 11.1, and a one-way valve member 11.3 fixed to the bottom of the liquid nitrogen tank 11.1 and detachably assembled to the top end of the tank base 11.2. The tank base 11.2 is communicated with the inner cavity of the sample chamber 6 through a pipeline. Among them, the tank base 11.2 includes a bottom cylinder 11.2.1 fixed to the middle of the bottom surface of the inner cavity of the cylindrical groove 4.2, an external thread pipe two 11.2.2 fixedly docked to the top end of the bottom cylinder 11.2.1, a hemispherical shell 11.2.3 fixedly docked to the top end of the external thread pipe two 11.2.2 at the bottom end, and a joint 11.2.5 sleeved on the side wall of the bottom cylinder 11.2.1 along the radial direction and connected to the nitrogen injection valve 6.3 one by one through a pipeline. A plurality of air inlet holes 11.2.4 are arranged circumferentially at the bottom of the hemispherical shell 11.2.3. The one-way valve member 11.3 includes a cylindrical shell 11.3.1 fixedly sleeved on the center of the bottom of the liquid nitrogen tank 11.1 and having a bottom end threadedly sleeved and matched with the external thread pipe two 11.2.2. A plurality of air outlet holes 11.3.1.1 are arranged circumferentially on the top side wall of the cylindrical shell 11.3.1 located in the inner cavity of the liquid nitrogen tank 11.1. A plug plate 11.3.2 is slidably sleeved vertically on the top of the inner cavity of the cylindrical shell 11.3.1. A second spring 11.3.3 is connected between the top surface of the plug plate 11.3.2 and the top surface of the inner cavity of the cylindrical shell 11.3.1. Among them, when assembling and connecting the liquid nitrogen tank 11.1 filled with liquid nitrogen to the tank base 11.2, the cylindrical shell 11.3.1 is gradually threadedly sleeved on the thread pipe two 11.2.2. After the threaded sleeving is in place, the hemispherical shell 11.2.3 pushes up the plug plate 11.3.2, so that the air outlet holes 11.3.1.1 are communicated with the air inlet holes 11.2.4, that is, the liquid nitrogen can be flushed into the inner cavity of the sample chamber 6 by opening the nitrogen injection valve 6.3. When disassembling the liquid nitrogen tank 11.1, due to the plug plate 11.3.2 being separated from the pushing action of the hemispherical shell 11.2.3, under the action of the second spring 11.3.3, it resets downward to close the outlet of the liquid nitrogen.

[0030] The turntable 12 in a ring structure is rotatably sleeved in the disc-shaped housing 5. A through hole 12.3 is provided near the outer edge of the disc-shaped housing 5. A sealing plate 12.4 is detachably sleeved in the through hole 12.3. Among them, the turntable 12 further includes an annular plate 12.1 rotatably sleeved and matched with the inner cavity of the disc-shaped housing 5 and an external tooth ring 12.2 fixed to the top surface of the annular plate 12.1. The sealing plate 12.4 and the through hole 12.3 can be threadedly sleeved.

[0031] The driving member 13 includes a motor 13.1 fixed to the top surface of the side housing 5.4 and having a power output shaft rotatably sleeved and matched with the shaft hole 5.5, and a gear 13.2 fixedly sleeved on the bottom end of the power output shaft of the motor 13.1 and meshing with the external tooth ring 12.2. That is, the driving member 13 is used to drive the turntable 12 to rotate, so that the through hole 12.3 on the turntable 12 is aligned with the corresponding sample chamber 6, facilitating the taking and placing of the sample container 7.

[0032] The top cover 14 is detachably fixed at the central hole 5.2. Among them, a plurality of knob screws 14.1 are threadedly sleeved along the vertical direction on the outer edge of the top cover 14, and a handle 14.2 is installed in the middle of the top surface of the top cover 14. The bottom of the knob screw 14.1 is threadedly sleeved with a screw hole in the annular groove at the central hole 5.2, so as to facilitate the disassembly and assembly of the top cover 14, thereby realizing the disassembly, assembly, taking and placing of the liquid nitrogen tank 11.1.

[0033] In summary, when operating to preserve pathological tissues, first fill a certain amount of preservation liquid into the cup body 7.1. At this time, the first spring 7.2.5 presses down tightly on the plug body 7.2.4, so the bottom of the cup body 7.1 is in a sealed state. Put the pathological tissue specimen collected by the digestive medicine department into the cup body 7.1 and cover the cup cover 7.3.

[0034] At this time, the through hole 12.3 is aligned with the sample chamber 6 to be placed. Remove the sealing plate 12.4, and the sample container 7 can be put into the sample chamber 6 through the through hole 5.1 and the through hole 12.3 in sequence. Among them, the sample container 7 is directly sleeved in the flexible mesh cover 6.4, and the whole sample container 7 is rotated so that the internal thread 7.2.1.1 is threadedly sleeved tightly with the corresponding external thread pipe 10.7.1.

[0035] Reassemble the sealing plate 12.4 in the through hole 12.3. The motor 13.1 drives the gear 13.2 to rotate, and the gear 13.2 drives the whole turntable 12 to rotate through the external tooth ring 12.2, so that a rotational airtight structure is provided between the bottom surface of the annular plate 12.1 of the sealing plate 12.4 and the top surface of the disc-shaped seat 4 of the just-placed sample container 7.

[0036] Then, the nitrogen injection valve 6.3 corresponding to the just-placed sample container 7 in the sample chamber 6 is opened to inject an appropriate amount of liquid nitrogen into the sample chamber 6 to achieve cryopreservation of the pathological tissue sample.

[0037] The liquid pump 8.1 and the corresponding liquid replenishing valve 10.3 are opened to replenish the preservation liquid in the sample container 7.

[0038] After the replenishment of the preservation liquid is completed, the air pump 9.1 and the air injection valve 10.6 are opened to inject sterile air into the preservation liquid in the sample container 7 through the microporous housing 7.2.3 to generate a large number of microbubbles to form a dynamic suspension environment. To avoid the compression deformation of the pathological tissue sample and avoid the damage to the pathological sample tissue caused by mechanical vibration, thereby ensuring the accuracy of the analysis of the pathological tissue sample.

[0039] When adding the preservation liquid or injecting sterile air into the sample container 7, the sterile air or preservation liquid with a certain pressure can overcome the first spring 7.2.5 upward to lift the plug body 7.2.4, so that the liquid path or the gas path is connected.

[0040] The preservation liquid components filled in the sample container 7 and the replenishing liquid tank 8 include phosphate buffer, paraformaldehyde, glutathione, vitamin C, gentamicin, glycerol and polyvinylpyrrolidone. Among them, 94.34% of phosphate buffer, 4% of paraformaldehyde, 0.1% of glutathione, 0.05% of vitamin C, 0.01% of gentamicin, 1% of glycerol and 0.5% of polyvinylpyrrolidone are taken by mass fraction. Phosphate buffer (PBS, pH 7.4) is used as the base liquid to maintain the stability of tissue osmotic pressure and avoid cell swelling or shrinkage; paraformaldehyde is used to replace formalin (formaldehyde solution) to reduce volatile toxicity, and at the same time prevent the damage of the acidic environment to the tissue by optimizing the pH (7.2 - 7.4); glutathione + vitamin C play a role in inhibiting oxidative stress, protecting the integrity of cell membranes and prolonging tissue activity; gentamicin is used as a broad-spectrum antibacterial agent to prevent sample corruption; glycerol is used to maintain the fluidity of cell membranes and reduce tissue sclerosis; polyvinylpyrrolidone (PVP) is used to increase the viscosity of the solution, assist the uniform penetration of the fixative and avoid local over-contraction. Formaldehyde and methanol are discarded, and a water-soluble formula is adopted to reduce the respiratory tract irritation and carcinogenic risk of operators. All components meet the ISO 10993 medical grade standard, and the waste liquid can be treated by conventional biodegradation to reduce the environmental burden.

[0041] In order to facilitate intelligent control, temperature sensors are attached to the inner wall of the sample chamber 6, pressure sensors are installed on the gas delivery pipe 10.5 and the infusion pipe 10.2 respectively, and the nitrogen injection valve 6.3, the replenishing liquid valve 10.3 and the gas injection valve 10.6 are all solenoid valves. That is, the nitrogen injection valve 6.3 is reasonably switched according to the temperature monitored by the temperature sensor in the sample chamber 6 to ensure the injection of an appropriate amount of liquid nitrogen to achieve the purpose of adjusting the temperature in the sample chamber 6. The opening and closing of the replenishing liquid valve 10.3 and the switch of the liquid pump 8.1 are controlled according to the detection value of the pressure sensor on the infusion pipe 10.2 to ensure the injection of an appropriate amount of preservation liquid into the sample container 7. The opening of the gas injection valve 10.6 and the air pump 9.1 are controlled by the detection value of the pressure sensor on the gas delivery pipe 10.5 to realize the injection of an appropriate amount of sterile air into the sample container 7. In addition, a pressure relief valve is set on the cup cover 7.3 to ensure the safety when taking and placing pathological tissues.

[0042] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0043] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A tissue preservation device for pathological analysis in the department of gastroenterology, comprising a mobile trolley (1), a cabinet body (2) fixed on the mobile trolley (1), and a sample container (7), characterized in that, Further included are: a disc-shaped seat (4), the bottom end of the disc-shaped seat (4) is fixedly sleeved on the middle part of the top plate of the cabinet body (2), a cylindrical groove (4.2) is arranged in the middle of the top surface of the disc-shaped seat (4), and a plurality of cylindrical cavities (4.1) are arranged near the outer edge; a disc-shaped shell (5), the bottom of the disc-shaped shell (5) is fixedly sleeved on the top end of the disc-shaped seat (4), and a central hole (5.2) and a through hole (5.1) are respectively arranged on the disc-shaped shell (5) corresponding to the cylindrical groove (4.2) and the cylindrical cavity (4.1); a sample chamber (6), the sample chamber (6) is fixedly sleeved in the cylindrical cavity (4.1); a liquid replenishing tank (8), the liquid replenishing tank (8) is arranged in the inner cavity of the cabinet body (2); a sterile gas tank (9), the sterile gas tank (9) is arranged in the inner cavity of the cabinet body (2); a gas-liquid distribution part (10), the gas path and the liquid path input ends of the gas-liquid distribution part (10) are respectively connected to the output ends of the sterile gas tank (9) and the liquid replenishing tank (8), and the combined structure of the gas path and the liquid path output ends of the gas-liquid distribution part (10) is sleeved on the center of the bottom of the sample chamber (6); a liquid nitrogen assembly (11), the liquid nitrogen assembly (11) includes a tank seat (11.2) fixed on the bottom surface of the cylindrical groove (4.2), a liquid nitrogen tank (11.1), and a one-way valve part (11.3) fixed on the bottom of the liquid nitrogen tank (11.1) and detachably assembled on the top end of the tank seat (11.2), and the tank seat (11.2) is communicated with the inner cavity of the sample chamber (6) through a pipeline; a turntable (12), the turntable (12) in a ring structure is rotatably sleeved in the disc-shaped shell (5), a through hole (12.3) is arranged near the outer edge of the disc-shaped shell (5), and a sealing plate (12.4) is detachably sleeved in the through hole (12.3); a driving part (13), the driving part (13) is used for driving the turntable (12) to rotate; a top cover (14), the top cover (14) is detachably fixed at the central hole (5.2); a gas-liquid input part (7.2) with the bottom sleeved on the sample container (7) and the bottom end sleeved on the combined structure of the gas path and the liquid path output ends of the gas-liquid distribution part (10), a microporous shell (7.2.3) is arranged at the top end of the gas-liquid input part (7.2), and the preservation liquid filled in the sample container (7) and the liquid replenishing tank (8) includes phosphate buffer solution, paraformaldehyde, glutathione, vitamin C, gentamicin, glycerol and polyvinylpyrrolidone.

2. The tissue preservation device for pathological analysis in the department of gastroenterology according to claim 1, characterized in that: The gas-liquid distributor (10) includes a cylindrical housing (10.1), a plurality of infusion tubes (10.2) whose inner ends are sleeved on the top side wall of the cylindrical housing (10.1) in the radial direction, a replenishing valve (10.3) sleeved on the outer ends of the infusion tubes (10.2), an annular housing (10.4) fixedly sleeved on the bottom of the cylindrical housing (10.1), a plurality of gas delivery tubes (10.5) whose inner ends are sleeved on the side wall of the annular housing (10.4) in the radial direction, an air injection valve (10.6) sleeved on the outer ends of the gas delivery tubes (10.5), and a vertical cylinder (10.7) whose bottom is sleeved with the output ends of the replenishing valve (10.3) and the air injection valve (10.6). The vertical cylinder (10.7) is sleeved at the center of the bottom of the sample chamber (6) and its top is sleeved with the bottom end of the gas-liquid input member (7.2). The bottom of the cylindrical housing (10.1) is connected to the output end of the replenishing tank (8) through a pipeline, and the bottom of the annular housing (10.4) is connected to the output end of the sterile gas tank (9) through a pipeline.

3. A tissue preservation device for pathological analysis in gastroenterology according to claim 2, characterized in that: An internally threaded tube (6.1) is fixedly sleeved at the center of the bottom of the sample chamber (6). The top end of the vertical cylinder (10.7) is butted with an externally threaded tube one (10.7.1) that is threadedly sleeved and matched with the internally threaded tube (6.1). A positioning ring (10.7.2) is fixed at the position of the top end of the vertical cylinder (10.7) corresponding to the bottom surface of the internally threaded tube (6.1). A locking nut (10.7.3) is threadedly sleeved at the position of the externally threaded tube one (10.7.1) corresponding to the top surface of the internally threaded tube (6.1).

4. The tissue preservation device for pathological analysis in gastroenterology according to claim 3, wherein: The sample container (7) includes a cup body (7.1) sleeved in the inner cavity of the sample chamber (6) and a cup cover (7.3) detachably covering the top end of the cup body (7.1). The gas-liquid input member (7.2) includes a vertical tube (7.2.1) whose top is fixedly sleeved at the center of the bottom of the cup body (7.1), a pressing ring (7.2.2) fixedly sleeved at the top end of the vertical tube (7.2.1) and whose bottom surface fits with the bottom surface of the inner cavity of the cup body (7.1), a plug body (7.2.4) arranged above the middle of the inner cavity of the vertical tube (7.2.1), and a first spring (7.2.5) whose bottom end is connected to the outer edge of the top surface of the plug body (7.2.4) and whose top end is connected to the inner wall of the top end of the vertical tube (7.2.1). An annular protrusion (7.2.1.2) that supports the bottom of the plug body (7.2.4) is provided in the middle of the inner peripheral wall of the vertical tube (7.2.1). An internal thread (7.2.1.1) that is threadedly sleeved and matched with the externally threaded tube one (10.7.1) is provided at the bottom end of the inner peripheral wall of the vertical tube (7.2.1). The microporous housing (7.2.3) is fixed at the top end of the vertical tube (7.2.1).

5. The tissue preservation device for pathological analysis in the department of gastroenterology according to claim 3, characterized in that: At the top of the liquid replenishing tank (8), there is a liquid pump (8.1) with an input end extending to the bottom of its inner cavity. The output end of the liquid pump (8.1) is connected to the bottom of the cylindrical shell (10.1) through a pipeline. At the top end of the side wall of the liquid replenishing tank (8), a liquid filling valve (8.2) is sleeved. At the top of the sterile gas tank (9), there is an air pump (9.1) with an input end extending into the inner cavity of the sterile gas tank (9). The output end of the air pump (9.1) is connected to the bottom of the annular shell (10.4) through a pipeline. At the top end of the side wall of the sterile gas tank (9), an air filling valve (9.2) is sleeved.

6. The tissue preservation device for pathological analysis in the department of gastroenterology according to claim 2, wherein: Between the cylindrical groove (4.2) and the surrounding cylindrical cavities (4.1), side holes (4.3) are respectively provided along the radial direction. At the position of the side wall of the sample chamber (6) corresponding to the side holes (4.3), an air inlet joint (6.2) is provided. A nitrogen injection valve (6.3) is sleeved at the end of the air inlet joint (6.2) passing through the side holes (4.3). The tank base (11.2) includes a bottom cylinder (11.2.1) with the bottom end fixed to the middle of the inner cavity bottom surface of the cylindrical groove (4.2), an external threaded pipe two (11.2.2) fixedly docked to the top end of the bottom cylinder (11.2.1), a hemispherical shell (11.2.3) with the bottom end fixedly docked to the top end of the external threaded pipe two (11.2.2), and joints (11.2.5) sleeved along the radial direction on the side wall of the bottom cylinder (11.2.1) and connected to the nitrogen injection valve (6.3) one by one through pipelines. A plurality of air inlet holes (11.2.4) are provided along the circumferential direction at the bottom of the hemispherical shell (11.2.3). The one-way valve member (11.3) includes a cylindrical shell (11.3.1) fixedly sleeved at the center of the bottom of the liquid nitrogen tank (11.1) and the bottom end threadedly sleeved and matched with the external threaded pipe two (11.2.2). On the top side wall of the cylindrical shell (11.3.1) located in the inner cavity of the liquid nitrogen tank (11.1), a plurality of air outlet holes ( 11.3.1.1) are provided. A plug plate (11.3.2) is slidably sleeved along the vertical direction on the top of the inner cavity of the cylindrical shell (11.3.1). A second spring (11.3.3) is connected between the top surface of the plug plate (11.3.2) and the top surface of the inner cavity of the cylindrical shell (11.3.1).

7. A tissue preservation device for pathological analysis in the department of gastroenterology according to claim 1, characterized in that: The turntable (12) further includes an annular plate (12.1) rotatably sleeved and matched with the inner cavity of the disc-shaped shell (5) and an external toothed ring (12.2) fixed to the top surface of the annular plate (12.1). One side of the disc-shaped shell (5) is fixedly connected with a side shell (5.4). A shaft hole (5.5) is provided at the center of the top surface of the side shell (5.4). The driving member (13) includes a motor (13.1) fixed to the top surface of the side shell (5.4) and a power output shaft rotatably sleeved and matched with the shaft hole (5.5), and a gear (13.2) fixedly sleeved at the bottom end of the power output shaft of the motor (13.1) and meshed with the external toothed ring (12.2).

8. A tissue preservation device for pathological analysis in gastroenterology according to claim 1, characterized in that: A flexible mesh cover (6.4) is fixed on the inner peripheral wall of the sample chamber (6), the sample container (7) is fixedly sleeved in the flexible mesh cover (6.4), and a rubber cushion plate (3) is provided between the mobile trolley (1) and the cabinet body (2).

9. The tissue preservation device for pathological analysis in the department of gastroenterology according to claim 6, characterized in that: A temperature sensor is attached to the inner wall of the sample chamber (6), pressure sensors are respectively installed on the gas delivery pipe (10.5) and the infusion pipe (10.2), and the nitrogen injection valve (6.3), the liquid supplement valve (10.3) and the gas injection valve (10.6) are all solenoid valves.

10. A tissue preservation device for pathological analysis in the department of gastroenterology according to claim 1, characterized in that: A plurality of knob screws (14.1) are vertically threadedly sleeved on the outer edge of the top cover (14), a handle (14.2) is installed in the middle of the top surface of the top cover (14), an annular groove which is sleeved and matched with the outer edge of the top cover (14) is provided at the outer edge of the top end of the central hole (5.2), and a screw hole which is threadedly sleeved and matched with the bottom end of the knob screw (14.1) is provided on the bottom surface of the annular groove.

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