Mango pollen preservation method

Abstract

The invention relates to the technical field of pollen preservation, in particular to a mango pollen preservation method which comprises the following steps: S1, nitrogen storage: selecting a double-layer metal container with the inner cavity specification of 60L-85L, and filling 30L of liquid nitrogen into an outer cavity of the metal container; step S2, seed selection: step S4, no-damage impurity removal: at a first stage, putting the mango pollen standing to normal temperature into an impurity remover 1, and driving the mango pollen to rotate and float up and down; and step S7, unfreezing for later use: in a second stage, placing the defrozen mango pollen in an artificial climate box with the humidity of 68-78% and the temperature of 29-34.5 DEG C, and incubating for 2-3.5 h. Mango pollen with excellent quality is selected and subjected to drying, impurity removal and refrigeration treatment in sequence, dormancy treatment of the mango pollen is achieved, and the lifting sieve plate capable of moving up and down in the container barrel in a reciprocating mode is arranged through the ball screw, so that gentle peeling and impurity removal are conveniently conducted on the mango pollen in the dynamic process.

Description

Technical Field

[0001] The present invention relates to the technical field of pollen preservation, and particularly relates to a method for preserving mango pollen. Background Art

[0002] Mango is one of the famous tropical fruits. Because of its delicate pulp and unique flavor, it is deeply loved by people, so it is known as the "King of Tropical Fruits". The inflorescence of mango is a panicle, terminal, large inflorescence, usually about 40 cm, bisexual or male flowers, small flowers, only 0.7 cm - 1 cm, each flower has 1 effective stamen, and few varieties have more than 2 effective stamens. Therefore, it is difficult to collect mango pollen. At present, the method for collecting mango pollen is to collect the anthers in the just-bloomed flowers one by one with tweezers, with a slow collection speed and low pollen viability.

[0003] Under the existing conditions, in order to improve the viability of mango pollen, the liquid nitrogen refrigeration method is often used for low-temperature treatment. For example, a non-contact mesh bag cryopreservation method for mango pollen disclosed in the publication number CN107926302A can extend the retention time of mango pollen viability from a normal few hours to 1 month, avoiding pollen rupture or internal structure damage caused by direct contact between pollen and liquid nitrogen. The breathable nylon mesh bag can also keep the pollen at a sufficient low temperature in the low-temperature gas environment of the liquid nitrogen tank. However, for the impurity removal process of mango pollen, the existing technology is adopted, and a violent stirring drive method is used for mango pollen, which is extremely easy to damage the structure of mango pollen. At the same time, the mango pollen to be refrigerated is not effectively and comprehensively pretreated, so that the mango pollen is extremely easy to be severely frozen in the low-temperature environment, seriously affecting the viability and survival rate of mango pollen. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem that it is difficult to preserve mango pollen in the prior art, and to propose a method for preserving mango pollen.

[0005] In order to achieve the above purpose, the present invention adopts the following technical scheme:

[0006] A method for preserving mango pollen, the preservation method comprising the following steps:

[0007] Step S1, nitrogen storage: Select a double-layer metal container with an inner cavity specification of 60L - 85L, and fill 30L of liquid nitrogen into the outer cavity of the metal container;

[0008] Step S2, seed selection:

[0009] In the first stage, select a transparent nylon mesh bag such that the mesh holes of the nylon mesh bag are smaller than mango pollen, and only one opening is reserved at the top position;

[0010] In the second stage, collect mature mango pollen when there is no dew, place it in a white shallow porcelain plate with a diameter of 10 cm - 12 cm, and manually pick out the plump mango pollen grains;

[0011] Step S3, dehydration and drying: Place the mangoes in a constant temperature drying oven at a temperature of 37°C - 42°C and continuously dry for 1.5 h - 2.5 h until the water content of the mango pollen reaches 5.5% - 7.2%;

[0012] Step S4, impurity removal without damage:

[0013] In the first stage, place the mango pollen that has been left to reach room temperature in a de-impurifier, and drive the mango pollen to rotate and float up and down;

[0014] In the second stage, screen out the small and slender impurities in the mango pollen during the dynamic process;

[0015] Step S5, sealed storage: Put the clean mango pollen into a nylon mesh bag with a specification of 200 ML and seal it;

[0016] Step S6, nitrogen refrigeration:

[0017] In the first stage, place the nylon mesh bag loaded with mango pollen in the inner cavity of a metal container;

[0018] In the second stage, extract the oxygen in the inner cavity of the metal container and achieve sealed cooling for 15 h - 18 h;

[0019] Step S7, thawing for use:

[0020] In the first stage, take out the frozen mango pollen and let it stand in a normal temperature environment for 8 h - 10.5 h;

[0021] In the second stage, place the thawed mango pollen in an artificial climate chamber with a humidity of 68% - 78% and a temperature of 29°C - 34.5°C and incubate for 2 h - 3.5 h.

[0022] Preferably, in the step S1, the inner cavity and the outer cavity of the metal container are arranged from the inside to the outside, and the outer cavity wraps the inner cavity.

[0023] Preferably, in the step S3, the mango pollen is statically dried in a paved manner, and the paving thickness of the mango pollen is less than 10 cm.

[0024] Preferably, the cleaner is fixedly installed with a driving motor, and a driving rotating shaft directly driven by the driving motor is rotatably installed at the middle position of the cleaner. A load-bearing sleeve with a hollow structure is rotatably installed at the upper end of the cleaner and is in transmission connection with the driving rotating shaft. An integrally connected container barrel with uniformly opened holes at the bottom is used for loading mango pollen on the load-bearing sleeve. A ball screw extending into the container barrel and driven by the driving rotating shaft is rotatably sleeved in the load-bearing sleeve. A lifting nut is adaptively installed on the ball screw, and a guiding ring groove is formed in the lifting nut. A guiding slider is slidably sleeved in the guiding ring groove. A guiding vertical groove is formed in the container barrel, and a lifting sieve plate is slidably sleeved in the guiding vertical groove. A traction support rod is connected between the guiding slider and the lifting sieve plate. A dust discharging plate is fixedly installed on the cleaner and is inclined.

[0025] Preferably, a driving gear and a driven gear which are meshed and connected are respectively key-connected to the driving rotating shaft and the load-bearing sleeve.

[0026] Preferably, a traction turntable is integrally connected to the driving rotating shaft, and an eccentric driving short yoke is integrally connected to the traction turntable. A transmission swing rod is rotatably installed on the cleaner, and a driven long hole for slidably sleeving the driving short yoke is formed at the upper end of the transmission swing rod. A driving long hole is formed at the lower end of the transmission swing rod. A guiding transverse groove is formed in the cleaner, and a reciprocating rack is slidably sleeved in the guiding transverse groove. A driven short yoke slidably sleeved in the driving long hole is integrally connected to the reciprocating rack. A reciprocating gear meshed and connected to the reciprocating rack is key-connected to the ball screw.

[0027] Preferably, in step S6, the nylon mesh bag is uniformly laid on the wall surface between the inner cavity and the outer cavity of the metal container.

[0028] Preferably, in step S7, a single-layer hollow plate is used to place the mango pollen after the first-stage thawing to avoid the melted ice water from soaking the mango pollen.

[0029] Compared with the prior art, the present invention has the following advantages:

[0030] 1. By selecting high-quality mango pollen and performing sequential drying, impurity removal, and refrigeration treatments on it, the present invention realizes the dormancy treatment of mango pollen and prolongs the life cycle of mango pollen.

[0031] 2. The present invention uses a cleaner to set a driving rotating shaft driven by a driving motor, and sets a load-bearing sleeve and a ball screw driven by the driving rotating shaft. The ball screw is used to set a lifting sieve plate that can move up and down reciprocally in the container barrel, so as to gently remove the skin and impurities of mango pollen during the dynamic process. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1Schematic flow chart of a method for preserving mango pollen proposed by the present invention;

[0033] Figure 2 Schematic structural diagram of a cleaner for a method for preserving mango pollen proposed by the present invention;

[0034] Figure 3 Cross-sectional view of a cleaner for a method for preserving mango pollen proposed by the present invention;

[0035] Figure 4 Enlarged schematic structural diagram of part A of a cleaner for a method for preserving mango pollen proposed by the present invention.

[0036] In the figure: 1. Cleaner; 2. Driving motor; 3. Driving rotating shaft; 4. Load-bearing sleeve; 5. Container barrel; 6. Ball screw; 7. Driving gear; 8. Driven gear; 9. Traction turntable; 901. Driving short yoke; 10. Transmission swing rod; 1001. Driven long hole; 1002. Driving long hole; 11. Guide transverse groove; 12. Reciprocating rack; 1201. Driven short yoke; 13. Reciprocating gear; 14. Lifting nut; 15. Guide ring groove; 16. Guide slider; 17. Guide vertical groove; 18. Lifting sieve plate; 19. Traction support rod; 20. Dust discharge plate. Detailed implementation manners

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0038] Referring to Figures 1 - 4 , a method for preserving mango pollen, the preservation method includes the following steps:

[0039] Step S1, nitrogen storage: Select a double-layer metal container with an inner cavity specification of 60L - 85L, and fill 30L of liquid nitrogen into the outer cavity of the metal container. It should be noted that by placing mango pollen in the inner cavity and liquid nitrogen in the outer cavity, while using the liquid nitrogen to cool the entire metal container, it can avoid the direct contact between the mango pollen and the liquid nitrogen, and prevent the liquid nitrogen with too low temperature from freezing the mango pollen;

[0040] Step S2, seed selection:

[0041] In the first stage, select a transparent nylon mesh bag, make the mesh holes of the nylon mesh bag smaller than the mango pollen, and only keep one opening at the top position. Use the nylon mesh bag to fill an appropriate amount of mango pollen to avoid over-packing and extrusion, so as to ensure the integrity of the mango pollen's own structure, and keep the pollen fully cooled in the low-temperature gas environment of the liquid nitrogen tank, achieving the purpose of maintaining the vitality of mango pollen for a long time;

[0042] In the second stage, collect mature mango pollen when there is no dew, and place it in a white shallow porcelain plate with a diameter of 10 cm to 12 cm. Manually pick out the plump mango pollen. It should be noted that by manual selection, it is possible to effectively avoid mango pollen that withers and dies due to lack of moisture or nutrients in the early stage. In particular, avoid using hard auxiliary tools to prevent damage to the delicate mango pollen structure. During the manual picking process, wear thin film gloves to reduce the erosion of human sweat on mango pollen and avoid mango pollen allergy at the same time;

[0043] Furthermore, mango tree species over five years old can be selected as the object for mango pollen collection to ensure the quality of mango pollen.

[0044] Step S3, dehydration and drying: Place the mango in a constant temperature drying oven at a temperature of 37°C - 42°C and continuously dry for 1.5 h - 2.5 h until the water content of the mango pollen reaches 5.5% - 7.2%;

[0045] Step S4, impurity removal without damage:

[0046] In the first stage, place the mango pollen that has been left to room temperature in the impurity remover 1, drive the mango pollen to rotate and float up and down. Instead of using the existing stirrer to roughly stir and drive the mango pollen, use a gentle vibration driving method so that the mango pollen can achieve skin peeling during the dynamic process and remove impurities during the peeling process;

[0047] In the second stage, screen out the small and slender impurities in the mango pollen during the dynamic process;

[0048] Step S5, sealed storage: Put the clean mango pollen into a nylon mesh bag with a specification of 200 ML and seal it. It should be noted that no more than 180 ML of mango pollen is placed in the 200 ML nylon mesh bag, and ensure that there is sufficient looseness between the individual mango pollen monomers. It is convenient to tie the top opening of the nylon mesh bag with a cotton rope, and at the same time use the cotton rope to carry a marking label for easy later management;

[0049] Step S6, nitrogen refrigeration:

[0050] In the first stage, place the nylon mesh bag containing mango pollen in the inner cavity of a metal container;

[0051] In the second stage, extract the oxygen in the inner cavity of the metal container and achieve sealed cooling for 15 h - 18 h. It should be noted that by reducing the oxygen content in the metal container and at the same time reducing the environmental temperature in the metal container, the mango pollen enters a dormant state under extreme conditions, reducing the life activity frequency of the mango pollen, thereby extending the entire life cycle;

[0052] Step S7, thawing for use:

[0053] In the first stage, the frozen mango pollen is taken out and placed in a room temperature environment for 8h-10.5h;

[0054] In the second stage, the thawed mango pollen is placed in an artificial climate box with a humidity of 68% to 78% and a temperature of 29°C to 34.5°C for incubation for 2h to 3.5h. Through three groups of comparative experiments, mango pollen with different vitality can be obtained using this storage method:

[0055] Vitality difference comparison experiment

[0056] pollen Whether to apply this preservation method Vitality (initial vitality 100%) The first group Yes 75.35％ The second group No 32.5％ The third group Yes 78.58％ The fourth group No 27.62％

[0057] In step S1, the inner cavity and the outer cavity in the metal container are arranged from inside to outside, and the outer cavity covers the inner cavity.

[0058] In step S3, the mango pollen is statically dried in a flattening manner, and the thickness of the flattened mango pollen is less than 10 cm, so as to avoid excessive accumulation of the mango pollen, thereby preventing the structural integrity of the mango pollen from being squeezed and damaged.

[0059] The drive motor 2 of the impurity remover 1 is fixedly installed, and the specific Figure 2 The impurity remover 1 is of a 匚-shaped structure as a whole, and a driving shaft 3 directly driven to rotate by a driving motor 2 is rotatably installed at the middle end of the impurity remover 1, and the driving shaft 3 is arranged horizontally. A load-bearing sleeve 4 with a hollow structure and transmission connection to the driving shaft 3 is rotatably installed at the upper end of the impurity remover 1. It should be noted that under the transmission action of the driving shaft 3, the load-bearing sleeve 4 rotates counterclockwise, and a container barrel 5 with uniform holes at the bottom end for loading mango pollen is integrally connected to the load-bearing sleeve 4, and a ball screw 6 extending into the container barrel 5 and driven by the driving shaft 3 is rotatably sleeved in the load-bearing sleeve 4. The load-bearing sleeve 4 and the ball screw 6 are both arranged longitudinally, and under the transmission action of the driving shaft 3, the ball screw 6 performs periodic bidirectional rotation. A lifting nut 14 is adapted to be installed on the ball screw 6, and a guide ring groove 15 is provided in the lifting nut 14, and a guide slider 16 is slidably mounted in the guide ring groove 15. Under the traction of the lifting screen plate 18, the guide slider 16 performs horizontal rotation in the guide ring groove 15. A guide vertical groove 17 is provided in the container barrel 5, and a lifting screen plate 18 is slidably mounted in the guide vertical groove 17. A traction support rod 19 is connected between the guide slider 16 and the lifting screen plate 18. Under the traction of the lifting nut 14, the lifting screen plate 18 is periodically moved up and down. An inclined dust removal plate 20 is fixedly installed on the impurity remover 1, and the dust removal plate 20 is used to receive the broken skin, debris and impurities discharged from the container barrel 5 to ensure the cleanliness of the mango pollen in the container barrel 5.

[0060] A driving rotating shaft 3 and a load-bearing sleeve 4 are respectively key-connected with a driving gear 7 and a driven gear 8 which are meshed and connected.

[0061] Specifically refer to the attached instruction manual Figure 2 A traction turntable 9 is integrally connected to the driving rotating shaft 3, and an eccentric driving short yoke 901 is integrally connected to the traction turntable 9. A transmission swing rod 10 is rotatably installed on the impurity remover 1, and a driven long hole 1001 for slidably sleeving the driving short yoke 901 is opened at the upper end of the transmission swing rod 10. Under the traction of the traction turntable 9, the transmission swing rod 10 swings reciprocally. A driving long hole 1002 is opened at the lower end of the transmission swing rod 10. A guiding transverse groove 11 is opened in the impurity remover 1, and a reciprocating rack 12 is slidably sleeved in the guiding transverse groove 11. A driven short yoke 1201 which is slidably sleeved in the driving long hole 1002 is integrally connected to the reciprocating rack 12. A reciprocating gear 13 which is meshed and connected to the reciprocating rack 12 is key-connected to the ball screw 6. The reciprocating rack 12 moving reciprocally drives the ball screw 6 to perform periodic bidirectional rotational motion through the reciprocating gear 13, so that the lifting nut 14 moves up and down.

[0062] In step S6, the nylon mesh bag is evenly laid on the wall surface between the inner cavity and the outer cavity of the metal container.

[0063] In step S7, a single-layer hollow plate is used to place the mango pollen thawed in the first stage to prevent the melted ice water from soaking the mango pollen. If the mango pollen at normal temperature is immersed in the low-temperature ice water for a long time, it is extremely easy to cause frostbite accidents. Timely discharging of the low-temperature ice water helps to protect the mango pollen from frost.

[0064] It should be noted that the specific model and specification of the driving motor 2 need to be selected and determined according to the actual specifications of the device, etc. The specific selection and calculation method adopts the existing technology in this field, so it will not be elaborated here.

[0065] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitution or change, and should be covered within the protection scope of the present invention.

Claims

1. A method for preserving mango pollen, characterized in that, The storage method comprises the following steps: Step S1, nitrogen storage: select a double-layer metal container with an inner cavity specification of 60L-85L, and fill 30L of liquid nitrogen into the outer cavity of the metal container; Step S2, seed selection: In the first stage, a transparent nylon mesh bag is selected, the mesh of which is smaller than the mango pollen, and only one end is left open at the top; In the second stage, mature mango pollen was collected when there was no dew, placed in a white shallow porcelain plate with a diameter of 10 cm to 12 cm, and the mango pollen with full grains was manually selected; Step S3, dehydration and drying: placing the mango in a constant temperature drying oven at a temperature of 37° C.-42° C., and drying for 1.5 h-2.5 h until the moisture content of the mango pollen reaches 5.5%-7.2%; Step S4, non-destructive cleaning: In the first stage, mango pollen that has been allowed to stand at room temperature is placed in a dust remover (1), and the mango pollen is driven to rotate and float up and down; In the second stage, small impurities in mango pollen are screened out in a dynamic process; Step S5, sealed storage: put the clean mango pollen into a nylon mesh bag with a specification of 200ML and seal it; Step S6, nitrogen refrigeration: In the first stage, a nylon mesh bag loaded with mango pollen was placed in the inner cavity of a metal container; In the second stage, oxygen is extracted from the inner cavity of the metal container and sealed cooling is achieved for 15h-18h; Step S7, thawing for standby use: In the first stage, the frozen mango pollen is taken out and placed in a room temperature environment for 8h-10.5h; In the second stage, the thawed mango pollen is placed in an artificial climate box with a humidity of 68% to 78% and a temperature of 29° C. to 34.5° C. for incubation for 2h to 3.5h.

2. The method for preserving mango pollen according to claim 1, wherein In step S1, the inner cavity and the outer cavity in the metal container are arranged from inside to outside, and the outer cavity covers the inner cavity.

3. A method for preserving mango pollen according to claim 1, characterized in that, In the step S3, the mango pollen is statically dried in a flattening manner, and the thickness of the flattened mango pollen is less than 10 cm.

4. A method for preserving mango pollen according to claim 1, characterized in that, The impurity remover (1) is fixedly mounted with a driving motor (2), and a driving shaft (3) driven to rotate directly by the driving motor (2) is rotatably mounted at the middle end of the impurity remover (1), and a load-bearing sleeve (4) with a hollow structure and drivingly connected to the driving shaft (3) is rotatably mounted at the upper end of the impurity remover (1), and a container barrel (5) having evenly opened holes at the bottom and used for loading mango pollen is integrally connected to the load-bearing sleeve (4), and a ball bearing extending into the container barrel (5) and driven by the driving shaft (3) is rotatably mounted in the load-bearing sleeve (4). The ball screw (6) is adapted to be mounted with a lifting nut (14), and a guide ring groove (15) is provided in the lifting nut (14), a guide slider (16) is slidably mounted in the guide ring groove (15), a guide vertical groove (17) is provided in the container barrel (5), and a lifting screen plate (18) is slidably mounted in the guide vertical groove (17), a traction support rod (19) is connected between the guide slider (16) and the lifting screen plate (18), and an inclined dust removal plate (20) is fixedly mounted on the impurity remover (1).

5. A method for preserving mango pollen according to claim 4, characterized in that, The driving shaft (3) and the load-bearing sleeve (4) are respectively key-connected with a driving gear (7) and a driven gear (8) that are meshed and connected.

6. A method for preserving mango pollen according to claim 4, characterized in that, A traction turntable (9) is integrally connected to the driving rotating shaft (3), and an eccentrically arranged driving short yoke (901) is integrally connected to the traction turntable (9). A transmission swing rod (10) is rotatably installed on the impurity remover (1), and a driven long hole (1001) for slidably sleeving the driving short yoke (901) is formed at the upper end of the transmission swing rod (10). A driving long hole (1002) is formed at the lower end of the transmission swing rod (10). A guiding transverse groove (11) is formed in the impurity remover (1), and a reciprocating rack (12) is slidably sleeved in the guiding transverse groove (11). A driven short yoke (1201) that is slidably sleeved in the driving long hole (1002) is integrally connected to the reciprocating rack (12). A reciprocating gear (13) that is meshed with the reciprocating rack (12) is key-connected to the ball screw (6).

7. A method for preserving mango pollen according to claim 1, characterized in that, In step S6, the nylon mesh bag is evenly laid on the wall surface between the inner cavity and the outer cavity of the metal container.

8. A method for preserving mango pollen according to claim 1, characterized in that, In step S7, a single-layer hollow plate is used to place the mango pollen that has undergone the first-stage thawing, so as to prevent the melted ice water from soaking the mango pollen.

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