Application of composition in fat reducing and oral beautifying functions

Abstract

The invention discloses an application of a composition in fat reducing and oral beautifying functions, and provides the application of the composition in the oral beautifying functions, the composition comprises MN-Gup bacterial powder, galactooligosaccharide and a white kidney bean extract, and specifically, the mass ratio of the MN-Gup bacterial powder to the galactooligosaccharide to the white kidney bean extract is 1: (0.5-10): (0.05-1). The invention further provides application of the composition in fat reduction, and it is verified that through mutual cooperation of the MN-Gup bacterial powder, the galactooligosaccharide and the white kidney bean extract in a specific proportion, the composition has a synergistic effect on fat reduction such as promotion of fat decomposition, inhibition of fat absorption and promotion of energy metabolism, and oral beauty such as brightening of skin color and light aging resistance.

Description

Technical Field

[0001] The present invention relates to the fields of fat reduction and oral beauty, and particularly to the application of a composition in fat reduction and oral beauty functions. Background Art

[0002] Obesity is a disease caused by excessive accumulation of fat in the body, leading to an increase in body weight. It is related to eating habits, drugs, reduced physical activity, and dysregulation of the gut microbiota structure. Currently, the main treatment methods for obesity include drug treatment, increased physical activity, and surgical treatment, etc., but the effects are not satisfactory or there are adverse side effects.

[0003] In addition, with the accelerating pace of people's work and life, mental stress is also increasing continuously. Coupled with many external factors such as bad living habits, poor environment, and ultraviolet rays, which have an adverse impact on the skin, more and more people are facing skin problems. Long-term exposure of the skin to ultraviolet rays can cause skin damage, skin photoaging, and even skin cancer and other skin lesions. Moreover, pigmentation is caused by reasons such as skin oxidation, changes in hormone levels, ultraviolet irradiation, and damaged skin barrier. Currently, for skin problems such as photoaging and pigmentation, the main treatments are the use of skin care products, facial masks, and various medical aesthetic treatments, but they often cannot fundamentally change the biological process of skin aging, and there are a series of potential side effects such as hormone imbalance.

[0004] Oral beauty has been a direction that has attracted much attention in the market in recent years. Oral beauty can improve the body's metabolism from the inside by ingesting bioactive ingredients, promote physical health and balance, and thus improve the skin condition, rather than simply solving the external manifestations of skin problems. Currently, there are some compositions of natural ingredients that have the effect of fat reduction or oral beauty. For example, the composition in CN202310056093.9 has the effect of fat reduction, and the composition in CN201710201371.X has the effect of oral beauty; but the main ingredients that play the role are all traditional Chinese medicine ingredients, which are not suitable for specific groups to take in terms of taste and safety. Summary of the Invention

[0005] Therefore, the purpose of the present invention is to provide a new composition with a wider range of applicable groups, which has both the functions of fat reduction and oral beauty.

[0006] The application of a composition in an oral beauty product, the composition comprising: MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract.

[0007] Among them, the strain in the MN-Gup bacterial powder is Bifidobacterium animalis subsp. lactis MN-Gup, provided by Mengniu High-tech Dairy Products (Beijing) Co., Ltd. Its source is the longevity village of Bama, Guangxi, known as the "Hometown of World Longevity". Its preservation number is CGMCC No. 15578, and it was deposited in the General Microbiology Center of the China Microbial Culture Collection Center on April 10, 2018. Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postcode: 100101; Galactooligosaccharides (GOS) was purchased from Great Ocean Ingredients PTY LTD; The white kidney bean extract can be self-extracted or a commercially available product purchased, for example: the white kidney bean extract purchased from Yunnan Tianbao Hua Biological Resources Development Co., Ltd.

[0008] The mass ratio of the MN-Gup bacterial powder, galactooligosaccharides, and white kidney bean extract is 1: 0.5 - 10: 0.05 - 1. For example: the mass ratio of the MN-Gup bacterial powder to galactooligosaccharides can be 1: 0.5, 1: 1, 1: 3, 1: 5, 1: 7, 1: 9, 1: 10 or a ratio not shown among any of the proportions. The mass ratio of the MN-Gup bacterial powder to the white kidney bean extract can be 1: 0.05, 1: 0.1, 1: 0.3, 1: 0.5, 1: 0.8, 1: 1 or a ratio not shown among any of the proportions. Preferably, the mass ratio of the MN-Gup bacterial powder, galactooligosaccharides, and white kidney bean extract is 1: 2 - 4: 0.1 - 0.3.

[0009] The viable count of Bifidobacterium MN-Gup in the composition is 3×10 9 CFU / g or more. For example: the viable count of Bifidobacterium animalis subsp. lactis MN-Gup in the composition can be 3×10 9 CFU / g, 4×10 9 CFU / g, 5×10 9 CFU / g, 6×10 9 CFU / g, 7×10 9 CFU / g, 8×10 9 CFU / g, 9×10 9 CFU / g, 10×10 9 CFU / g, 20×10 9 CFU / g, 30×10 9 CFU / g, 50×10 9 CFU / g, 80×10 9 CFU / g, 100×10 9 CFU / g, 150×10 9 CFU / g, 200×109 CFU / g, 250×10 9 CFU / g, 300×10 9 CFU / g, 350×10 9 CFU / g, 400×10 9 CFU / g, 450×10 9 CFU / g, 500×10 9 CFU / g, 600×10 9 CFU / g, etc. or contents not shown between any viable counts among them. Preferably, the viable count of MN-Gup bacteria in the composition is 4×10 9 ~500×10 9 CFU / g.

[0010] The oral beauty product is an oral beauty product for brightening skin tone or / and an oral beauty product for anti-photoaging.

[0011] The present invention also discloses the application of the above composition in the preparation of a fat-reducing product. Further, the composition reduces fat by promoting fat decomposition and energy metabolism. Further, the composition reduces fat by inhibiting fat absorption.

[0012] The technical solution of the present invention has the following advantages: 1. The present invention provides a new composition, specifically, including MN-Gup bacteria powder, galactooligosaccharide, and white kidney bean extract; through the mutual cooperation of MN-Gup bacteria powder, galactooligosaccharide, and white kidney bean extract in the composition, an oral beauty effect of brightening skin tone and anti-photoaging is achieved; and it can also achieve the effect of reducing fat by promoting fat decomposition and / or energy metabolism and inhibiting fat absorption.

[0013] 2. The composition provided by the present invention further optimizes the ratio of MN-Gup bacteria powder, galactooligosaccharide, and white kidney bean extract to 1:0.5~10:0.05~1. Through the mutual cooperation of galactooligosaccharide, Bifidobacterium animalis subsp. lactis MN-Gup, and white kidney bean extract, a synergistic effect is achieved in the oral beauty efficacy of brightening skin tone and anti-photoaging; and at the same time, a synergistic effect is also achieved in promoting fat decomposition and / or energy metabolism, inhibiting fat absorption, and then achieving the effect of reducing fat.

[0014] 3. The components of the present invention's solution are simple and easy to obtain, do not contain traditional Chinese medicine components, have high safety, are applicable to a wider group of people, and the taste is easy to formulate, and can be widely used in various products such as fat reduction and oral beauty. Description of the Drawings

[0015] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0016] Figure 1 It is a graph of the experimental results of the fat decomposition promoting effect of the present invention; Figure 2 It is a graph of the experimental results of the fat absorption inhibiting effect of the present invention; Figure 3 It is a graph of the experimental results of the energy metabolism promoting effect of the present invention; Figure 4 It is a graph of the experimental results of the skin brightening effect of the present invention; Figure 5 It is a graph of the experimental results of the anti-photoaging effect of the present invention. Specific Embodiments

[0017] The following embodiments are provided to better further understand the present invention. They are not limited to the best embodiments, and do not limit the content and protection scope of the present invention. Any product that is the same as or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior art features falls within the protection scope of the present invention.

[0018] For those not specifying specific experimental steps or conditions in the embodiments, the operations or conditions of the conventional experimental steps described in the literature in this field can be followed. For the reagents or instruments not specifying the manufacturer, they are all conventional reagent products that can be obtained through commercial purchase.

[0019] Example 1 A composition includes MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract with a mass ratio of 1:0.5:0.05.

[0020] The strain in the MN-Gup bacterial powder in the present invention is Bifidobacterium animalis subsp. lactis MN-Gup, provided by Mengniu High-Tech Dairy Products (Beijing) Co., Ltd. Its source is the longevity village of Bama in Guangxi, the "hometown of world longevity". Its preservation number is CGMCC No. 15578, and it was preserved in the China General Microbiological Culture Collection Center on April 10, 2018. Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postcode: 100101. The viable count content of the MN-Gup bacterial powder used in this experiment is 2×10 11CFU / g; The galactooligosaccharide (GOS) used in the present invention was purchased from Great Ocean Ingredients PTY LTD; the white kidney bean extract (BYD) used in the present invention was purchased from Yunnan Tianbao Birch Bio-Resources Development Co., Ltd.

[0021] Example 2 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:3:0.2.

[0022] Example 3 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:10:1.

[0023] Example 4 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:0.1:0.01.

[0024] Example 5 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:100:10.

[0025] Example 6 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:10:0.05.

[0026] Example 7 A composition comprising MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract in a mass ratio of 1:0.5:1.

[0027] Experimental Example 1. Experimental Animals Zebrafish were all raised in fish culture water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, the conductivity was 450 - 550 μS / cm; pH was 6.5 - 8.5; hardness was 50 - 100 mg / L CaCO3).

[0028] 2. Detection Method 2.1 Determination of the Maximum Test Concentration (MTC) Determination of MTC for promoting lipolysis efficacy: Randomly select albino zebrafish with melanin allele mutations at 2 dpf and place them in a 6-well plate. Each well (experimental group) is treated with 30 zebrafish. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup viable bacteria concentration samples (concentrations are shown in Table 1) are dissolved in water and given to each well respectively. At the same time, a normal control group is set up, and the volume of each well is 3 mL. After treatment at 28 °C for 1 day, Nile red dye is dissolved in water and given to each experimental group. Continue to treat at 28 °C until 4 dpf, and measure the MTC of the samples on normal zebrafish.

[0029] Determination of MTC for inhibiting fat absorption efficacy: Randomly select wild-type AB strain zebrafish at 5 days post-fertilization (5 dpf) and place them in a beaker. Each beaker (experimental group) is treated with 30 zebrafish. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup viable bacteria concentration samples (concentrations are shown in Table 2) are dissolved in water and given to each well respectively. At the same time, a normal control group and a model control group are set up, and the volume of each cup is 20 mL. After treatment at 28 °C for 1 h, except for the normal control group, the remaining concentration groups are dissolved in water and given egg yolk powder to feed the zebrafish to establish a food fat absorption model. After continuing to treat at 28 °C for 30 h, wash away the samples, and place them in the morning at 7 dpf to measure the MTC of the samples on the model zebrafish.

[0030] Determination of MTC for promoting energy metabolism efficacy: Randomly select wild-type AB strain zebrafish at 6 hours post-fertilization (6 hpf) and place them in a 6-well plate. Each well (experimental group) is treated with 30 zebrafish. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup viable bacteria concentration samples (concentrations are shown in Table 3) are dissolved in water and given to each well respectively. At the same time, a normal control group is set up, and the volume of each well is 3 mL. After treatment at 28 °C for 6 days, measure the MTC of the samples on normal zebrafish.

[0031] Determination of MTC for skin brightening efficacy: Randomly select albino zebrafish with melanin allele mutations at 3 days post-fertilization (3 dpf) and place them in a 6-well plate. Each well (experimental group) is treated with 30 zebrafish. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup viable bacteria concentration samples (concentrations are shown in Table 4) are dissolved in water and given to each well respectively. At the same time, a normal control group and a model control group are set up, and the volume of each well is 3 mL. After treatment at 28 °C for 2 h, except for the normal control group, the remaining experimental groups are dissolved in water and given menadione to establish a zebrafish pigmentation model. After continuing to treat at 28 °C for 22 h, measure the MTC of the samples on the model zebrafish.

[0032] Measurement of the anti-photoaging efficacy by MTC: Wild-type AB strain zebrafish at 3 days post-fertilization (3 dpf) were randomly selected and placed in 6-well plates, with 30 zebrafish in each well (experimental group). Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup viable bacteria concentration samples (concentrations are shown in Table 5) were dissolved in water and given to each well. At the same time, a normal control group and a model control group were set up, and the volume of each well was 3 mL. After treatment at 28 °C for 3 h, except for the normal control group, a zebrafish caudal fin shrinkage model was established by simulating sunlight irradiation in the remaining experimental groups. After continuing to treat at 28 °C for 1 day, the MTC of the samples on the model zebrafish was measured.

[0033] 2.2 Evaluation of the effect of the composition on promoting fat decomposition Melanin allele mutant zebrafish (albino) at 2 dpf were randomly selected and placed in 6-well plates, with 30 zebrafish in each well (experimental group). The compound group samples of Examples 1-7 were dissolved in water and given. Examples 1-7 corresponded to FP1~FP7 in sequence. At the same time, a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group, and a BYD group were set up. The volume of each well was 3 mL, and the total concentration of the intervention samples in each group was 200 μg / mL. The volume of each well was 3 mL. After treatment at 28 °C for 1 day, except for the normal control group (NC), Nile red dye was dissolved in water and given to each experimental group. After continuing to treat at 28 °C until 4 dpf, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The NIS-Elements D 3.20 advanced image processing software was used to analyze and collect data, and the fluorescence intensity of the yolk sac fat of zebrafish was analyzed. The statistical analysis results of this index were used to evaluate the efficacy of the samples in promoting fat decomposition. The statistical treatment results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis between two groups. p<0.05 indicated that the difference was statistically significant.

[0034] 2.3 Evaluation of the effect of the composition on inhibiting fat absorption Randomly select 5 dpf wild-type AB strain zebrafish in a beaker, with 30 zebrafish treated in each beaker (experimental group). The compound group sample of Example 2 was administered by water solution and named FP2. At the same time, a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group, and a BYD group were set up. The volume of each well was 3 mL, and the total concentration of the intervention samples in each group was 200 μg / mL. The volume of each beaker was 20 mL. After treatment at 28 °C for 1 h, except for the normal control group (NC), zebrafish in the other concentration groups were fed with egg yolk powder by water solution to establish a food fat absorption model. After continuing to treat at 28 °C for 30 h, the samples were washed off. Oil red O was given for whole-body fat staining at 7 dpf in the morning. Randomly select 10 zebrafish from each experimental group and place them under a dissection microscope for photographing. Use NIS-Elements D 3.20 advanced image processing software to collect data, analyze the fat staining intensity of the intestine and tail blood vessels, and evaluate the fat absorption inhibitory effect of the sample based on the statistical analysis results of this index. The statistical processing results are expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis between two groups. p < 0.05 indicates that the difference is statistically significant.

[0035] 2.4. Evaluation of the effect of the composition on promoting energy metabolism Randomly select 6 hpf wild-type AB strain zebrafish in a 6-well plate, with 30 zebrafish treated in each well. The compound group samples of Examples 1-3 were administered by water solution. Examples 1-3 corresponded to FP1-FP3 in sequence. At the same time, a normal control group (NC), an MN-Gup group, a GOS group, and a BYD group were set up. The volume of each well was 3 mL, and the total concentration of the intervention samples in each group was 200 μg / mL. After treatment at 28 °C for 6 days, zebrafish samples were collected according to the instructions of the coenzyme NAD(H) content detection kit, and the contents of NAD+ and NADH in zebrafish were detected using a multifunctional microplate reader. Analyze the NAD+ / NADH ratio of zebrafish in each experimental group, and evaluate the effect of the sample on the energy metabolism rate based on the statistical analysis results of this index. The statistical processing results are expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis. p < 0.05 indicates that the difference is statistically significant.

[0036] 2.5. Evaluation of the effect of the composition on brightening skin color Randomly select 3 dpf zebrafish of the melanin allele mutant Albino strain into 6-well plates, with 30 zebrafish treated in each well (experimental group). The compound group samples of Examples 1-7 were administered in water solution. Examples 1-7 corresponded to FP1-FP7 in sequence. At the same time, a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group, and a BYD group were set up. The volume of each well was 3 mL, and the total concentration of the intervention samples in each group was 200 μg / mL. After treatment at 28°C for 2 h, except for the normal control group, menadione was administered in water solution to the rest of the experimental groups to establish a zebrafish pigmentation model. After continuing to treat at 28°C for 22 h, 10 zebrafish were randomly selected from each experimental group and placed under a dissection microscope for photographing. The NIS-Elements D 3.20 advanced image processing software was used to analyze and collect data, and the pigment signal intensity of the zebrafish yolk sac was analyzed. The statistical analysis results of this index were used to evaluate the skin brightening effect of the sample. The statistical processing results were expressed as mean ± SE, and SPSS 26.0 software was used for statistical analysis. p<0.05 indicated that the difference was statistically significant.

[0037] 2.6, Evaluation of the effect of the composition on anti-photoaging Randomly select 3 dpf wild-type AB strain into 6-well plates, with 30 zebrafish treated in each well. The compound group samples of Examples 1-3 were administered in water solution. Examples 1-3 corresponded to FP1-FP3 in sequence. At the same time, a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group, and a BYD group were set up. The volume of each well was 3 mL, and the total concentration of the intervention samples in each group was 200 μg / mL. After treatment at 28°C for 3 h, except for the normal control group, the rest of the experimental groups were irradiated with simulated sunlight to establish a zebrafish caudal fin shrinkage model. After continuing to treat at 28°C for 1 day, 10 zebrafish were randomly selected from each experimental group and placed under a dissection microscope for photographing. The NIS-Elements D 3.20 advanced image processing software was used to analyze and collect data, and the area of the zebrafish caudal fin was analyzed. The statistical analysis results of this index were used to evaluate the anti-wrinkle effect of the sample. The statistical processing results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis. p<0.05 indicated that the difference was statistically significant.

[0038] 3. Experimental results 3.1. The determination results of the maximum detection concentration (MTC) are shown in Tables 1-5 below.

[0039] Table 1

[0040] Table 2

[0041] Table 3

[0042] Table 4

[0043] Table 5

[0044] In the intervention range of 12.5 - 200 μg / mL of white kidney bean extract, the states of zebrafish in each efficacy experimental group were similar to those in the normal control group. Therefore, under the experimental conditions of this experiment, the MTC of white kidney bean extract for the effects of promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, brightening skin tone, and anti-photoaging was 200 μg / mL. In the intervention range of 125 - 2000 μg / mL of galactooligosaccharide (GOS), the states of zebrafish in the experimental group were similar to those in the normal control group. Therefore, under the experimental conditions of this experiment, the MTC of galactooligosaccharide for the effects of promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, brightening skin tone, and anti-photoaging was 2000 μg / mL. For MN-Gup bacterial powder, in the intervention dose range of 1×10 5 CFU / mL - 1×10 8 CFU / mL, the states of zebrafish in the experimental group were similar to those in the normal control group. However, in the group with 1×10 9 CFU / mL, deaths occurred in all 5 effects of promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, brightening skin tone, and anti-photoaging, and the mortality rates were 17%, 100%, 80%, 13%, and 40% respectively. Therefore, under the experimental conditions of this experiment, the MTC of MN-Gup bacterial powder for each efficacy was 1×10 8 CFU / mL.

[0045] 3.2. The experimental results of the composition's effect on promoting fat decomposition are as Figure 1 shown.

[0046] Figure 1 In it, *: significantly different compared with the normal control group (NC) (P < 0.05); #: significantly different compared with the MN-Gup group (P < 0.05); &: significantly different compared with the GOS group (P < 0.05); ￥: significantly different compared with the BYD group (P < 0.05).

[0047] Through Figure 1It can be seen that the fluorescence intensity of fat in the yolk sac of the NC group was 4,750,000 ± 230,000 pixels, and those of the MN-Gup group, GOS group, and BYD group were 3,450,000 ± 330,000 pixels, 3,240,000 ± 190,000 pixels, and 3,270,000 ± 220,000 pixels respectively. There were significant differences between the MN-Gup group, GOS group, BYD group and the NC group, indicating that each of the single components of MN-Gup, GOS, and BYD had the effect of promoting fat decomposition. In the compound groups, the fluorescence intensities of fat in the yolk sac of the FP1-FP7 groups were 2,630,000 ± 130,000 pixels, 2,550,000 ± 150,000 pixels, 2,530,000 ± 160,000 pixels, 3,750,000 ± 190,000 pixels, 3,120,000 ± 180,000 pixels, 2,540,000 ± 140,000 pixels, and 2,630,000 ± 150,000 pixels respectively. The fluorescence intensities of fat in the yolk sac of the FP1-FP7 groups were significantly lower than those of the NC group, indicating that the composition in the present invention had the effect of promoting fat decomposition; moreover, there were significant differences between the FP1-FP3 groups and the FP6-FP7 groups and the MN-Gup group, GOS group, and BYD group, indicating that the composition composed of MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract with a mass ratio of 1:0.5-10:0.05-1 had a synergistic effect in promoting fat decomposition.

[0048] 3.3. Experimental results of the composition's inhibition of fat absorption are as Figure 2 shown.

[0049] Figure 2 In the figure, *: significant difference compared with the model control group (MC) (P < 0.05); #: significant difference compared with the MN-Gup group (P < 0.05); &: significant difference compared with the GOS group (P < 0.05); ￥: significant difference compared with the BYD group (P < 0.05).

[0050] It can be seen through Figure 2 that the fat staining intensity of the intestine and tail blood vessels in the NC group was 25,700 ± 800 pixels, that in the MC group was 92,100 ± 2,900 pixels, and the MC group was significantly higher than the NC group, indicating that the model was successfully established. The fat staining intensities of the intestine and tail blood vessels in the MN-Gup group, GOS group, and BYD group were 78,800 ± 3,200 pixels, 72,800 ± 2,100 pixels, and 70,900 ± 3,000 pixels respectively, which were significantly lower than those in the MC group, indicating that the three single components had the effect of inhibiting fat absorption. The fat staining intensity of the intestine and tail blood vessels in the FP2 group was 62,000 ± 2,800 pixels, which was significantly lower than that in the MC group and significantly lower than those in the MN-Gup group, GOS group, and BYD group, indicating that the composition composed of MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract with a mass ratio of 1:2-4:0.1-0.3 had a synergistic effect in inhibiting fat absorption.

[0051] 3.4. Experimental results of the composition's promotion of energy metabolism are asFigure 3 as shown

[0052] Figure 3 In it, *: there was a significant difference compared with the normal control group (NC) (P < 0.05); #: there was a significant difference compared with the MN-Gup group (P < 0.05); &: there was a significant difference compared with the GOS group (P < 0.05); ¥: there was a significant difference compared with the BYD group (P < 0.05).

[0053] By Figure 3 it can be seen that the NAD + / NADH ratio of the NC group was 1.17 ± 0.09, and the NAD + / NADH ratios of the MN-Gup group, GOS group, and BYD group were 1.69 ± 0.10, 1.30 ± 0.07, and 1.32 ± 0.12 respectively. The MN-Gup group was significantly higher than the NC group, while there was no significant difference between the GOS group, BYD group and the NC group, indicating that the MN-Gup group had the effect of promoting energy metabolism. In the compound groups, the NAD + / NADH ratios of the FP1 - FP3 groups were 2.62 ± 0.12, 2.18 ± 0.06, and 2.04 ± 0.02 respectively, all significantly higher than that of the NC group, indicating that FP1 - FP3 had the effect of promoting energy metabolism. Moreover, the NAD + / NADH ratios of the FP1 - FP3 groups were all significantly higher than those of the MN-Gup group, GOS group, and BYD group, indicating that the three components had a synergistic effect in promoting energy metabolism within the ratio range of 1:0.5 - 10:0.05 - 1.

[0054] 3.5. The experimental results of the skin brightening effect of the composition are as Figure 4 shown

[0055] Figure 4 In it, *: there was a significant difference compared with the model control group (MC) (P < 0.05); #: there was a significant difference compared with the MN-Gup group (P < 0.05); &: there was a significant difference compared with the GOS group (P < 0.05); ¥: there was a significant difference compared with the BYD group (P < 0.05).

[0056] By Figure 4It can be seen that the yolk sac pigment signal intensity of the NC group was 24,900 ± 900 pixels, that of the MC group was 37,200 ± 1,600 pixels. The yolk sac pigment signal intensity of the MC group was significantly higher than that of the NC group, indicating that the modeling was successful. The yolk sac pigment signal intensities of the MN-Gup group, GOS group, and BYD group were 27,500 ± 1,100 pixels, 29,800 ± 1,000 pixels, and 30,300 ± 1,100 pixels respectively, which were significantly lower than that of the MC group, indicating that each single component of MN-Gup, GOS, and BYD had the effect of brightening the skin color. In the compound groups, the yolk sac pigment signal intensities of the FP1-FP7 groups were 19,500 ± 1,100 pixels, 20,000 ± 500 pixels, 24,300 ± 600 pixels, 29,900 ± 1,000 pixels, 29,600 ± 1,200 pixels, 23,100 ± 700 pixels, and 20,000 ± 900 pixels respectively. The yolk sac pigment signal intensities of the FP1-FP7 groups were all significantly lower than that of the MC group, indicating that the compositions in the present invention all had the effect of brightening the skin color. Moreover, there were significant differences between FP1-FP3 and FP6-FP7 and the MN-Gup group, GOS group, and BYD group, indicating that the composition composed of MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract with a mass ratio of 1:0.5-10:0.05-1 had a synergistic effect on brightening the skin color.

[0057] 3.6. The experimental results of the anti-photoaging efficacy of the composition are as Figure 5 shown.

[0058] Figure 5 In the figure, *: significant difference compared with the model control group (MC) (P < 0.05); #: significant difference compared with the MN-Gup group (P < 0.05); &: significant difference compared with the GOS group (P < 0.05); ￥: significant difference compared with the BYD group (P < 0.05).

[0059] Through Figure 5It can be seen that the caudal fin area of the NC group was 79.87 ± 1.00×10⁴ pixels, that of the MC group was 56.56 ± 1.36×10⁴ pixels. The caudal fin area of the MC group was significantly lower than that of the NC group, indicating that simulated sunlight irradiation caused shrinkage of the zebrafish caudal fin and the model was successfully established. The caudal fin areas of the MN-Gup group, GOS group, and BYD group were 64.42 ± 1.49×10⁴ pixels, 63.65 ± 1.55×10⁴ pixels, and 62.37 ± 0.83×10⁴ pixels respectively, which were significantly higher than that of the MC group, indicating that each of MN-Gup, GOS, and BYD had the effect of resisting photoaging. In the compound groups, the caudal fin areas of the FP1 - FP3 groups were 70.24 ± 1.47×10⁴ pixels, 72.11 ± 1.84×10⁴ pixels, and 68.84 ± 0.86×10⁴ pixels respectively. The FP1 - FP3 groups were significantly higher than the MC group, indicating that they had the effect of resisting photoaging, and there were significant differences compared with the MN-Gup group, GOS group, and BYD group, indicating that the FP1 - FP3 compositions had a synergistic effect on the effect of resisting photoaging within the mass ratio range of 1:0.5 - 10:0.05 - 1.

[0060] Obviously, the above embodiments are merely examples for clear illustration and not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. Use of a composition in an oral beauty product, characterized in that, The composition comprises: MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract.

2. The application according to claim 1, wherein The mass ratio of the MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract is 1: 0.5-10: 0.05-1.

3. The application according to claim 2, wherein The viable count of MN-Gup bacteria in the composition is 3×10 9 CFU / g or more.

4. The application according to claim 3, characterized in that, The viable count of MN-Gup bacteria in the composition is 4×10 9 ~500×10 9 CFU / g.

5. The application according to any one of claims 1-4, characterized in that, The mass ratio of the MN-Gup bacterial powder, galactooligosaccharide, and white kidney bean extract is 1: 2-4: 0.1-0.

3.

6. The application according to any one of claims 1 to 4, characterized in that The oral beauty product is an oral beauty product for brightening skin tone or / and an oral beauty product for anti-photoaging.

7. The application according to any one of claims 1-4, characterized in that, The said composition also has an application in the preparation of a fat-reducing product.

8. The application according to claim 7, wherein The composition reduces fat by promoting fat decomposition and energy metabolism.

9. The application according to claim 7, characterized in that The composition reduces fat by inhibiting fat absorption.

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