Medical adhesive for promoting wound healing and preparation method thereof

Abstract

The invention discloses a preparation method of a medical adhesive for promoting wound healing, which comprises the following steps: S1, preparing graphene oxide into GO sol, mixing the GO sol with bioactive glass, a high polymer material and a porous structure forming agent after surface modification, and calcining after gelatinization to obtain a GO-BAG nano composite material; s2, the GO-BAG nano composite material is subjected to a reaction with alkynyl bromide, and an alkynylated GO-BAG nano composite material is obtained; then, the GO-BAG nano composite material reacts with phenolated antibacterial peptide, and the surface modified GO-BAG nano composite material is obtained; s3, mixing with a polylactic acid-based biodegradable polymer, and adding a dynamic cross-linking agent for reaction to obtain an adhesive matrix; and S4, compounding with cyanoacrylate and an auxiliary agent to obtain the medical adhesive. According to the preparation method disclosed by the invention, the adhesive not only has excellent adhesion performance and biocompatibility, but also has excellent antibacterial performance, and can meet rapid and safe adhesion requirements under the conditions of different skin types and wounds.

Description

Technical Field

[0001] The present invention relates to the technical field of adhesives, and particularly to a medical adhesive for promoting wound healing and a preparation method thereof. Background Art

[0002] In the medical field, medical adhesives for promoting wound healing are one of the commonly used wound closure and skin repair materials in clinics. An ideal medical adhesive for promoting wound healing should have strong adhesion and good biocompatibility, and at the same time have antibacterial properties to reduce the risk of infection.

[0003] However, traditional medical adhesives for promoting wound healing often cannot take into account the above functional effects, and there is still room for improvement in some aspects. As is known to those skilled in the art, if the adhesive has insufficient adhesion performance, it is difficult to adapt to different skin types and wound shape changes, resulting in poor wound closure effect. If the adhesive has deficiencies in biocompatibility and antibacterial properties, it is likely to cause skin irritation or allergic reactions, which is not conducive to wound healing and increases the possibility of wound infection.

[0004] In summary, there is still room for improvement in the existing medical adhesives for promoting wound healing to meet the clinical needs. It is urgent to develop new adhesives to overcome the above problems and improve the wound treatment effect and patient experience. Summary of the Invention

[0005] In view of the above deficiencies of the prior art, the present invention provides a preparation method of a medical adhesive for promoting wound healing to solve the technical problems existing in the traditional medical adhesives for promoting wound healing in terms of adhesion performance, biocompatibility and antibacterial properties.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0007] A preparation method of a medical adhesive for promoting wound healing, the preparation method comprising the following steps:

[0008] S1: Disperse graphene oxide (GO) in an ethanol aqueous solution, add an appropriate amount of acidic substance and stir evenly to form a stable GO sol. Then add a phosphate ester compound for surface modification of GO. Mix the modified GO sol with bioactive glass particles, and add a polymer material and a porous structure former. After stirring evenly, let it stand at room temperature to gelify. After drying, calcining, and washing, a GO-BAG nanocomposite with a porous structure is obtained. In step S1 of this application, after the graphene oxide (GO) is modified by a phosphate ester compound, the interaction with calcium ions in the bioactive glass (BAG) is enhanced, promoting the release of calcium and phosphate ions, providing minerals for skin cell proliferation and migration, accelerating wound healing, and at the same time forming a more stable GO-BAG composite structure to play a synergistic advantage. In addition, graphene oxide has good thermal conductivity and can effectively conduct and disperse heat. In the application of a medical adhesive for promoting wound healing, this property helps to regulate the local temperature of the wound and create a more suitable environment for wound healing. Adding a porous structure former and combining with calcination treatment significantly increases the specific surface area and porosity of the GO-BAG nanocomposite, enhances the surface activity, is conducive to interacting with biological fluids, and promotes cell adhesion and growth. The calcination treatment optimizes the microstructure of the material, removes impurities, makes the pores more uniform and the structure more stable, thus showing excellent performance in the medical adhesive for promoting wound healing.

[0009] S2: Disperse the GO-BAG nanocomposite obtained in step S1 in an ethanol solution, add alkynyl bromide, and react to obtain an alkynylated GO-BAG nanocomposite. Then mix the phenolated antimicrobial peptide with the alkynylated GO-BAG nanocomposite, and through an alkynyl-phenol click reaction, modify the antimicrobial peptide on the surface of the GO-BAG nanocomposite to obtain a surface-modified GO-BAG nanocomposite. In step S2 of this application, by performing a click reaction between the phenolated antimicrobial peptide and the GO-BAG nanocomposite treated with alkynyl bromide, a structure capable of fixing the antimicrobial peptide is formed on the material surface. This structure enables the antimicrobial peptide to continuously play an antibacterial role during the use of the adhesive, inhibits bacterial growth, reduces the risk of infection, creates a sterile healing environment for the wound, and accelerates recovery. At the same time, the modification of the antimicrobial peptide improves the biocompatibility of the nanocomposite, making it safer and non-irritating when in contact with the skin, and solving the problems of insufficient antibacterial and biocompatibility of traditional adhesives.

[0010] S3: Using the polylactic acid-based biodegradable polymer as the matrix material, uniformly disperse the surface-modified GO-BAG nanocomposite obtained in step S2 in the solution by the solution blending method, and add a dynamic crosslinking agent to react to obtain an adhesive matrix with dynamic adhesion performance. In step S3 of this application, by mixing the surface-modified GO-BAG nanocomposite with the polylactic acid-based biodegradable polymer and adding a dynamic crosslinking agent, an adhesive matrix with a dynamic crosslinked network structure is formed. This structure endows the adhesive matrix with good flexibility and reversible adhesion performance, enabling it to dynamically adjust according to the wound shape and skin physiological activities, better adapting to skin stretching and contraction, improving the adhesion effect and usage comfort. At the same time, the polylactic acid-based biodegradable polymer can be degraded into non-toxic small molecules in the body, absorbed or excreted by the human body, and does not need to be removed after use, reducing the pain and risk of patients.

[0011] S4: Compound the adhesive matrix obtained in step S3 with cyanoacrylate and additives, after mixing and stirring evenly, carry out drying treatment, and finally obtain the medical adhesive for promoting wound healing. In step S4 of this application, by compounding the adhesive matrix with dynamic adhesion performance with cyanoacrylate and additives, the comprehensive performance of the medical adhesive for promoting wound healing is further optimized. Cyanoacrylate has rapid curing and strong adhesion performance, and synergistically acts with the adhesive matrix to form a more stable adhesion system, significantly enhancing the adhesion strength and stability of the adhesive, enabling it to quickly and firmly adhere to the wound. The addition of additives improves the properties of the adhesive such as moisture retention, anti-corrosion, and antioxidant properties, making it more stable and reliable during use, and capable of better meeting the usage requirements under different environments and conditions. In addition, the compounded medical adhesive for promoting wound healing has good fluidity and operability, is convenient to apply on the wound surface, and can quickly cure to form a uniform adhesive film layer, improving the convenience and efficiency of use, and solving the problems of insufficient comprehensive performance and inconvenient use of traditional adhesives.

[0012] As a preferred technical solution, the phosphate compound is a mixture of triethyl phosphate and sodium dihydrogen phosphate and / or disodium hydrogen phosphate.

[0013] As a preferred technical solution, the porous structure former is at least one of cetyltrimethylammonium bromide, dodecyltrimethylammonium bromide, and sodium dodecyl sulfate.

[0014] As a preferred technical solution, the alkynyl bromide is at least one of 3-bromopropyne, 4-bromobutyne, and 5-bromo-1-pentyne.

[0015] As a preferred technical solution, the preparation method of the phenolated antimicrobial peptide is: dissolve the antimicrobial peptide in an appropriate amount of deionized water, add a phenolating reagent, a coupling agent, and a catalyst, and stir and react to obtain the phenolated antimicrobial peptide.

[0016] As a preferred technical solution, the polylactic acid-based biodegradable polymer is at least one of polylactic acid, poly(lactic-co-glycolic acid), and poly(lactic-co-caprolactone).

[0017] As a preferred technical solution, the dynamic cross-linking agent is at least one of polyethylene glycol-polylysine block copolymer, polyethylene glycol-polyaspartic acid block copolymer, and polyethylene glycol-polyglutamic acid block copolymer.

[0018] As a preferred technical solution, the cyanoacrylate is at least one of methyl α-cyanoacrylate, ethyl α-cyanoacrylate, propyl α-cyanoacrylate, butyl α-cyanoacrylate, isobutyl α-cyanoacrylate, octyl α-cyanoacrylate, and isooctyl α-cyanoacrylate.

[0019] As a preferred technical solution, the auxiliary agent is at least one of a humectant, a preservative, an antioxidant, a lubricant, an antifoaming agent, and a blood coagulation factor.

[0020] Another aspect of the present invention is to provide a medical adhesive for promoting wound healing, which is prepared by the method for preparing a medical adhesive for promoting wound healing as described above.

[0021] Advantages of the present invention:

[0022] The method for preparing a medical adhesive for promoting wound healing according to the present invention, through the innovative operation of modifying the surface of graphene oxide with a phosphate compound, combined with the introduction of a porous structure former and subsequent calcination treatment, and at the same time performing surface antibacterial peptide modification and constructing a dynamic cross-linking network structure on the nanocomposite material, not only realizes multi-faceted improvement of the defects of traditional medical adhesives for promoting wound healing in terms of adhesion performance, biocompatibility, antibacterial performance, etc., but also realizes the optimization of the wound healing environment and the significant improvement of the patient's use experience. The method for preparing a medical adhesive according to the present invention not only enhances the interaction between the adhesive and the skin and the antibacterial ability, but also maintains the stability and biodegradability of the material, helps to accelerate the wound healing process, reduce the risk of infection, and reduce secondary harm to the patient.

[0023] Generally speaking, the medical adhesive for promoting wound healing according to the present invention not only has excellent comprehensive properties such as adhesion strength, moisture retention, antiseptic property, antioxidant property, etc., but also has good flexibility, reversible adhesion property, and biodegradability, can meet the adhesion requirements under different skin types and wound conditions, and provides a new solution for the rapid, safe, and effective healing of skin wounds. Specific embodiments

[0024] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments in the following description are only examples, and those skilled in the art can think of other obvious variants.

[0025] Example 1

[0026] The preparation method of the medical adhesive for promoting wound healing in this embodiment includes the following steps:

[0027] S1: Disperse 1.8 g of graphene oxide (GO) in 25 mL of ethanol aqueous solution (the volume ratio of ethanol to deionized water is 1:1), add 1 mL of concentrated hydrochloric acid (mass fraction 37%) and stir evenly to form a stable GO sol. Then add 0.3 g of phosphate compound and stir and react at room temperature for 1 hour for GO surface modification. The phosphate compound is composed of triethyl phosphate and sodium dihydrogen phosphate with a mass ratio of 2:1; mix the modified GO sol with 6.8 g of bioactive glass particles, add 3 g of polyethylene glycol and 0.25 g of porous structure former dodecyltrimethylammonium bromide, stir evenly and let it gel at room temperature for 24 hours; put the gel into an oven and dry it at 60°C for 4 hours, then place it in a muffle furnace and heat it up to 300°C at a heating rate of 5°C / min and calcine for 2 hours, and finally wash it 3 times with deionized water, soak for 1 hour each time, to obtain a GO-BAG nanocomposite with a porous structure.

[0028] S2: Disperse 8 g of the GO-BAG nanocomposite obtained in step S1 in 50 mL of ethanol, add 1.8 g of 3-bromopropyne, stir and react for 24 hours to obtain an alkynylated GO-BAG nanocomposite; then mix 2.5 g of phenolated antimicrobial peptide with the alkynylated GO-BAG nanocomposite, stir and react at room temperature for 4 hours, and modify the antimicrobial peptide on the surface of the GO-BAG nanocomposite through an alkynyl-phenol click reaction to obtain a GO-BAG nanocomposite with surface-modified antimicrobial peptide.

[0029] S3: Use 50 g of poly(lactic-co-glycolic acid) (PLGA) as the matrix material, dissolve it in 150 mL of ethyl acetate, and uniformly disperse 8 g of the GO-BAG nanocomposite with surface-modified antimicrobial peptide obtained in step S2 in the PLGA solution by solution blending method, and add 20 g of a dynamic crosslinking agent (polyethylene glycol-polylysine block copolymer), stir and react for 1 hour to obtain an adhesive matrix with dynamic adhesion performance.

[0030] S4: Mix 66 g of the adhesive matrix obtained in step S3 with 22 g of methyl α-cyanoacrylate and 12 g of auxiliary agents. The auxiliary agents include 3.5 g of hyaluronic acid (moisturizer), 1.5 g of phenoxyethanol (preservative), 2.5 g of ascorbic acid (antioxidant), 1.5 g of magnesium stearate (lubricant), 1 g of polydimethylsiloxane (defoamer), and 2 g of thrombin (coagulation factor). After mixing and stirring evenly, dry at 40 °C for 2 hours to finally obtain the medical adhesive for promoting wound healing.

[0031] The preparation method of the phenolated antimicrobial peptide is as follows: Dissolve 5 g of the antimicrobial peptide in 50 mL of deionized water, add 2.5 g of a phenolated reagent (phenethylphenol), 1 g of a coupling agent (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, EDC), and 0.5 g of a catalyst (N-hydroxysuccinimide, NHS), and stir and react at room temperature for 1 hour to obtain the phenolated antimicrobial peptide.

[0032] Example 2

[0033] The preparation method of the medical adhesive for promoting wound healing in this example includes the following steps:

[0034] S1: Disperse 2 g of graphene oxide (GO) in 25 mL of an ethanol aqueous solution (volume ratio of ethanol to deionized water is 1:1), add 1 mL of concentrated hydrochloric acid (mass fraction of 37%) and stir evenly to form a stable GO sol. Then add 0.33 g of a phosphate compound and stir and react at room temperature for 1 hour for GO surface modification. The phosphate compound is composed of triethyl phosphate and sodium dihydrogen phosphate with a mass ratio of 3:2; Mix the modified GO sol with 7 g of bioactive glass particles, add 4 g of polyvinyl alcohol and 0.3 g of the porous structure former sodium dodecyl sulfate, stir evenly and let it gel at room temperature for 24 hours; Put the gel in an oven and dry at 60 °C for 4 hours, then place it in a muffle furnace and heat it to 300 °C at a heating rate of 5 °C / min and calcine for 2 hours, and finally wash it 3 times with deionized water, soaking for 1 hour each time, to obtain a GO-BAG nanocomposite with a porous structure.

[0035] S2: Disperse 8.5 g of the GO-BAG nanocomposite obtained in step S1 in 50 mL of ethanol, add 2.1 g of 4-bromobutyne, and stir and react for 24 hours to obtain an alkynylated GO-BAG nanocomposite; Then mix 3 g of the phenolated antimicrobial peptide with the alkynylated GO-BAG nanocomposite and stir and react at room temperature for 4 hours. Through an alkynyl-phenol click reaction, the antimicrobial peptide is modified on the surface of the GO-BAG nanocomposite to obtain a GO-BAG nanocomposite with an antimicrobial peptide surface modification. The preparation method of the phenolated antimicrobial peptide is the same as that in Example 1.

[0036] S3: Using 55 g of poly (lactic-co-glycolic acid) (PLGA) as the matrix material, dissolve it in 150 mL of ethyl acetate. By the solution blending method, uniformly disperse 9 g of the GO-BAG nanocomposite material with surface-modified antibacterial peptide obtained in step S2 in the PLGA solution, and add 22 g of a dynamic cross-linking agent (polyethylene glycol-polyaspartic acid block copolymer). Stir and react for 1 hour to obtain an adhesive matrix with dynamic adhesion performance.

[0037] S4: Compound 68 g of the adhesive matrix obtained in step S3 with 20 g of ethyl α-cyanoacrylate and 12 g of an auxiliary agent, where the auxiliary agent is the same as in Example 1.

[0038] Example 3

[0039] The preparation method of the medical adhesive for promoting wound healing in this example includes the following steps:

[0040] S1: Disperse 2.2 g of graphene oxide (GO) in 25 mL of an ethanol aqueous solution (volume ratio of ethanol to deionized water is 1:1), add 1 mL of concentrated hydrochloric acid (mass fraction is 37%) and stir evenly to form a stable GO sol. Then add 0.35 g of a phosphate compound and stir and react at room temperature for 1 hour for GO surface modification. The phosphate compound is composed of triethyl phosphate and disodium hydrogen phosphate with a mass ratio of 5:2. Mix the modified GO sol with 7.2 g of bioactive glass particles, add 5 g of polyethylene glycol and 0.35 g of the porous structure former cetyltrimethylammonium bromide. After stirring evenly, let it stand at room temperature for 24 hours to gel. Put the gel into an oven and dry it at 60 °C for 4 hours, then place it in a muffle furnace and heat it to 300 °C at a heating rate of 5 °C / min and calcine for 2 hours. Finally, wash it 3 times with deionized water, soak for 1 hour each time, to obtain a GO-BAG nanocomposite material with a porous structure.

[0041] S2: Disperse 9 g of the GO-BAG nanocomposite material obtained in step S1 in 50 mL of ethanol, add 2.5 g of 5-bromo-1-pentyne, stir and react for 24 hours to obtain an alkynylated GO-BAG nanocomposite material. Then mix 3.5 g of phenolated antibacterial peptide with the alkynylated GO-BAG nanocomposite material and stir and react at room temperature for 4 hours. Through the alkynyl-phenol click reaction, modify the antibacterial peptide on the surface of the GO-BAG nanocomposite material to obtain a GO-BAG nanocomposite material with surface-modified antibacterial peptide. The preparation method of the phenolated antibacterial peptide is the same as in Example 1.

[0042] S3: Using 60 g of poly (lactic-co-glycolic acid) (PLGA) as the matrix material, dissolve it in 150 mL of ethyl acetate. By solution blending method, uniformly disperse 10 g of the surface-modified antibacterial peptide GO-BAG nanocomposite obtained in step S2 in the PLGA solution, and add 25 g of a dynamic cross-linking agent (polyethylene glycol-polyglutamic acid block copolymer). Stir and react for 1 hour to obtain an adhesive matrix with dynamic adhesion performance.

[0043] S4: Compound 70 g of the adhesive matrix obtained in step S3 with 18 g of n-propyl α-cyanoacrylate and 12 g of an auxiliary agent, and the auxiliary agent is the same as in Example 1.

[0044] Comparative Example 1

[0045] The preparation method of the medical adhesive for promoting wound healing in this comparative example is basically the same as that in Example 1 in terms of raw material composition and preparation steps. The difference is that in the preparation method of this comparative example, graphene oxide is not added in step S1.

[0046] Comparative Example 2

[0047] The preparation method of the medical adhesive for promoting wound healing in this comparative example is basically the same as that in Example 1 in terms of raw material composition and preparation steps. The difference is that in the preparation method of this comparative example, phosphate ester compounds are not added in step S1.

[0048] Comparative Example 3

[0049] The preparation method of the medical adhesive for promoting wound healing in this comparative example is basically the same as that in Example 1 in terms of raw material composition and preparation steps. The difference is that in the preparation method of this comparative example, a porous structure former is not added in step S1, and the calcination operation is not carried out at the same time.

[0050] Comparative Example 4

[0051] The preparation method of the medical adhesive for promoting wound healing in this comparative example is basically the same as that in Example 1 in terms of raw material composition and preparation steps. The difference is that in the preparation method of this comparative example, the GO-BAG nanocomposite in step S2 is not subjected to alkynylation treatment, and the antibacterial peptide is not subjected to phenylation treatment, that is, the two are directly mixed and added, rather than being added by alkyne-phenol click reaction.

[0052] Perform performance tests on the medical adhesives for promoting wound healing prepared in Examples 1 to 3 and Comparative Examples 1 to 4, and the performance results are shown in Table 1:

[0053] Among them, the test method for the adhesion strength is as follows: First, evenly apply the medical adhesive on a standard test plate to form an adhesive layer with a uniform thickness (0.5 mm ± 0.1 mm), and cure it in an environment of 25°C and a relative humidity of 50% for 24 hours. Subsequently, use a universal material testing machine to fix the test plate in the fixture and conduct a tensile test at a tensile speed of 10 mm / min, and record the maximum tensile force. Finally, the result is calculated through the formula "Adhesion strength (MPa) = Maximum tensile force (N) / Adhesion area (m 2 )".

[0054] The test method for biocompatibility is as follows: Prepare a sample of the medical adhesive in a standard size and test it using the L929 cell line. The culture medium is DMEM supplemented with 10% fetal bovine serum. After co-culturing the sample with the cells for 24 hours, use an MTT kit to detect the cell viability and calculate the cell survival rate. The cell toxicity evaluation criteria are as follows: A cell survival rate ≥ 80% indicates no cell toxicity, 60% - 80% indicates mild cell toxicity, and < 60% indicates significant cell toxicity.

[0055] The test method for antibacterial property is as follows: Prepare a sample of the medical adhesive in a standard size (1 cm × 1 cm × 0.5 cm) and use Staphylococcus aureus (ATCC 6538) or Escherichia coli (ATCC 8739) to prepare a bacterial suspension with a concentration of 1×10 6 CFU / mL. Place the sample in a petri dish containing 10 mL of nutrient broth, add 1 mL of the bacterial suspension, and then incubate it in a constant temperature incubator at 37°C. Take samples at 24 hours, 48 hours, and 72 hours respectively. After rinsing the sample with sterile normal saline, grind it into a suspension, and calculate the number of viable bacteria by the plate counting method. The formula for calculating the antibacterial rate is: "Antibacterial rate = (Number of viable bacteria in the control group - Number of viable bacteria in the experimental group) / Number of viable bacteria in the control group × 100%", and the control group is a blank sample without adding the medical adhesive.

[0056] The test method for wound healing ability is as follows: Apply the medical adhesive on the skin wound of an animal model (rat), with the wound area being 1 cm 2 . Observe the wound healing condition after 7 days, measure the change in the wound area, and calculate the healing rate.

[0057] The judgment criteria for the healing time are as follows:

[0058] Complete healing: It is defined as the wound area reducing to less than 10% of the initial area, and there are no obvious exudation, redness, swelling and other inflammatory reactions on the wound surface.

[0059] Partial healing: It is defined as the wound area reducing to 30% - 10% of the initial area, but there are still certain degrees of inflammatory reactions.

[0060] Unhealed: The reduction in wound area is less than 30% of the initial area, and there is an obvious inflammatory response.

[0061] The test method for hemostatic performance is as follows: Apply the medical skin adhesive to the skin wound of an animal model (rat). The wound area is 1 cm 2 , and the wound depth is 2 mm. Record the wound bleeding time (the time from wound formation to bleeding cessation), use a stopwatch to record the time, and calculate the average hemostatic time. Table 1

[0062] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification is only the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.

Claims

1. A preparation method of a medical adhesive for promoting wound healing, characterized in that, The preparation method comprises the following steps: S1: Graphene oxide is dispersed in an ethanol aqueous solution, an appropriate amount of acidic substance is added dropwise and stirred evenly to form a stable GO sol, and then a phosphate compound is added for surface modification of GO; the modified GO sol is mixed with bioactive glass particles, and a polymer material and a porous structure former are added, and after stirring evenly, it is allowed to gel at room temperature; after drying, calcining, and washing, a GO-BAG nanocomposite with a porous structure is obtained; S2: The GO-BAG nanocomposite obtained in step S1 is dispersed in an ethanol solution, alkynyl bromide is added, and an alkynylated GO-BAG nanocomposite is obtained by reaction; then the phenolated antibacterial peptide is mixed with the alkynylated GO-BAG nanocomposite, and through an alkynyl-phenol click reaction, the antibacterial peptide is surface-modified on the GO-BAG nanocomposite to obtain a surface-modified GO-BAG nanocomposite; S3: Using a polylactic acid-based biodegradable polymer as a matrix material, the surface-modified GO-BAG nanocomposite obtained in step S2 is uniformly dispersed in a solution by a solution blending method, and a dynamic cross-linking agent is added, and a binder matrix with dynamic adhesion performance is obtained by reaction; S4: The binder matrix obtained in step S3 is compounded with cyanoacrylate and additives, and after mixing and stirring evenly, a drying treatment is carried out, and finally the medical adhesive for promoting wound healing is obtained.

2. The method for preparing a medical adhesive for promoting wound healing according to claim 1, wherein The phosphate compound is a mixture of triethyl phosphate and sodium dihydrogen phosphate and / or disodium hydrogen phosphate.

3. The preparation method of the medical adhesive for promoting wound healing according to claim 1, wherein, The porous structure former is at least one of cetyltrimethylammonium bromide, dodecyltrimethylammonium bromide, and sodium dodecyl sulfate.

4. The method for preparing a medical adhesive for promoting wound healing according to claim 1, wherein The alkynyl bromide is at least one of 3-bromopropyne, 4-bromobutyne, and 5-bromo-1-pentyne.

5. The preparation method of the medical adhesive for promoting wound healing according to claim 1, wherein The preparation method of the phenolated antibacterial peptide is: dissolving the antibacterial peptide in an appropriate amount of deionized water, adding a phenolated reagent, a coupling agent, and a catalyst, and obtaining the phenolated antibacterial peptide after stirring and reacting.

6. The preparation method of the medical adhesive for promoting wound healing according to claim 1, wherein, The polylactic acid-based biodegradable polymer is at least one of polylactic acid, poly(lactic acid-glycolic acid) copolymer, and poly(lactic acid-caprolactone) copolymer.

7. The preparation method of the medical adhesive for promoting wound healing according to claim 1, wherein, The dynamic cross-linking agent is at least one of polyethylene glycol-polylysine block copolymer, polyethylene glycol-polyaspartic acid block copolymer, and polyethylene glycol-polyglutamic acid block copolymer.

8. The preparation method of the medical adhesive for promoting wound healing according to claim 1, wherein, The cyanoacrylate is at least one of methyl α-cyanoacrylate, ethyl α-cyanoacrylate, propyl α-cyanoacrylate, butyl α-cyanoacrylate, isobutyl α-cyanoacrylate, octyl α-cyanoacrylate, and isooctyl α-cyanoacrylate.

9. The method for preparing a medical adhesive for promoting wound healing according to claim 1, characterized in that, The additive is at least one of a humectant, a preservative, an antioxidant, a lubricant, an antifoaming agent, and a coagulation factor.

10. A medical adhesive for promoting wound healing, characterized in that, The adhesive is prepared by using the preparation method of the medical adhesive for promoting wound healing according to any one of claims 1 to 9.

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