Continuous quantitative dropwise adding device for experimental reagent

Abstract

The invention discloses an experimental reagent continuous quantitative dropwise adding device which comprises a reagent container, the reagent container is provided with a reagent storage part and a reagent output part, the input end of the reagent output part is provided with an elastic one-way piece used for controlling a reagent to flow out downwards, and the output end of the reagent output part is provided with a flow dividing piece; the dropping container is provided with a containing inner cavity and an elastic assembly, the containing inner cavity comprises a single dropping storage cavity with an insertion end and a dropping end, the insertion end is used for allowing the reagent output part to penetrate through in a sliding mode, and the dropping end is provided with a dropping hole communicated with the outside; a blocking assembly which is configured to keep the dripping hole closed in a non-working state and release blocking of the dripping hole in a working state is arranged in the single dripping storage cavity; the control mechanism comprises a dropping control assembly which is used for providing external force for promoting the reagent container to move downwards so as to enable the single dropping storage cavity to generate negative pressure, and the blocking assembly relieves blocking of the dropping hole under the action of the negative pressure. The device can be used for continuously and quantitatively dropwise adding reagents so as to meet the requirements of large-batch specimen pathological experiments.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental reagent adding equipment, and particularly to a device for continuously and quantitatively dripping experimental reagents. Background Art

[0002] Pathological technology is an important branch of basic pathology for pathological diagnosis, and is used to observe and study pathological changes in tissue cell morphology. It mainly includes tissue sectioning and staining experimental techniques (special staining, cell preparation, immunofluorescence, immunohistochemistry, etc.). During these experimental processes, it is usually necessary to drip reagents onto tissue specimens for experimental operations. The pathological staining experimental steps are cumbersome and various reagents are used. Most experimental links have high requirements for the dripping amount of reagents, and it is necessary to quantitatively add reagents according to the experimental purpose. Too much or too little dripping amount of reagents will affect the experimental results, and too much will also cause waste of reagents. For example, eosin is dripped for coloring during small specimen tissue sampling, various staining solutions are dripped during special staining and cell liquid-based preparation, and antibodies are dripped during immunohistochemistry and immunofluorescence processes. Currently, experimental tools such as pipettes, dropping bottles, and rubber-tipped droppers are usually used for reagent dripping. In clinical pathological work, it is usually necessary to conduct large-scale specimen experiments simultaneously (dozens to hundreds of tissue specimens). Although a pipette can achieve accurate quantitative dripping of reagents, since each reagent dripping requires 3 steps of sucking reagents (from the reagent bottle), transferring, and releasing reagents (to the experimental group), when conducting large-scale specimen pathological experiments, its limitations and deficiencies in repeatedly sucking and transferring reagents are particularly obvious, reducing work efficiency, and thus affecting the timeliness of experimental results. In addition, although experimental tools such as dropping bottles and rubber-tipped droppers can complete reagent dripping, the single-time reagent dripping amount mainly depends on the subjective judgment of the experimental operator (different forces result in different squeezing amplitudes of the rubber head), there is a problem of inaccurate quantification, and there are also limitations similar to those of pipettes in repeatedly sucking and transferring reagents. These processes are cumbersome and time-consuming, and are not conducive to the efficient development of clinical work.

[0003] Generally speaking, the current solutions still have problems such as low work efficiency, inaccurate quantification, and waste of reagents. It is necessary to design a device for continuously, quantitatively, portably, and simply operating for rapid quantitative reagent dripping to meet the needs of large-scale specimen pathological experiments. Summary of the Invention

[0004] In view of the above defects existing in the prior art, the present invention provides a device for continuously and quantitatively dripping experimental reagents that is continuous, quantitative, portable, and simple to operate to meet the needs of large-scale specimen pathological experiments.

[0005] The present invention is implemented by adopting the following technical solutions:

[0006] A device for continuously and quantitatively dripping experimental reagents, comprising:

[0007] A reagent container having a reagent storage portion and a reagent output portion communicating with the reagent storage portion, wherein an input end of the reagent output portion is configured with an elastic one-way member for controlling downward flow of the reagent, and an output end thereof is configured with a shunt member for shunting and discharging the reagent and allowing air to flow upward;

[0008] A dropping container having a receiving inner cavity for receiving the reagent container, the receiving inner cavity being configured with an elastic component for causing the reagent container to reciprocate in the vertical direction inside it. The receiving inner cavity includes a single-drop storage cavity having an insertion end through which the reagent output portion slides and a dropping end, and a dropping hole communicating with the outside is provided at the dropping end. A blocking component is provided in the single-drop storage cavity, and the blocking component is configured to keep the dropping hole closed in a non-working state and release the blocking of the dropping hole in a working state;

[0009] A control mechanism including a dropping control component for providing an external force to cause the reagent container to move downward, so as to generate a negative pressure in the single-drop storage cavity, and the blocking component releases the blocking of the dropping hole under the action of the negative pressure, so that the reagent in the single-drop storage cavity flows out from the dropping hole.

[0010] Further, the control mechanism further includes a quantitative control component configured to drive the reagent container to move up and down in the receiving inner cavity in a quantifiable manner, so as to quantitatively adjust different positions of the reagent output portion in the single-drop storage cavity, and further quantitatively adjust the volume of the single-drop storage cavity.

[0011] Further, the quantitative control component includes a pushing member and an adjusting member for controlling the quantifiable movement of the pushing member in the vertical direction, and the pushing member is arranged above the top of the reagent container in a manner capable of moving in the vertical direction.

[0012] Further, the dropping control component includes a dropping pressing member arranged above the reagent container in a manner capable of moving in the vertical direction, and when receiving a downward pressing operating force, the dropping pressing member transmits the operating force to the reagent container.

[0013] Further, the control mechanism further includes a control housing detachably coupled to the upper portion of the dropping container. The quantitative control component and the dropping control component are integrated on the control housing. The upper portion of the reagent container is received inside the control housing. An installation hole is formed in the upper end surface of the control housing. The pusher has an installation portion threadedly engaged with the installation hole. The bottom end of the installation portion is provided with a pushing portion located inside the control housing. The top end of the installation portion is threadedly connected to the adjusting member. Scale lines indicating its movement amount are shown on the outer wall of the installation portion. The dropping pressing member includes a dropping contact portion located inside the control housing and below the pushing portion, and a dropping rod portion vertically protruding from the upper surface of the dropping contact portion. The dropping rod portion sequentially passes through the pushing portion, the installation portion, and the adjusting member and then extends outside the control housing.

[0014] Further, a diversion member and an elastic one-way member are sequentially arranged from top to bottom in the reagent output portion. The output end of the elastic one-way member is provided with a lip piece that maintains its normally closed state by elasticity. The diversion member is provided with a diversion hole. One end of the diversion hole communicates with the reagent storage portion, and the other end communicates with the input end of the elastic one-way member.

[0015] Further, the shunt member includes a shunt vertical portion inserted into the reagent output portion and a shunt end portion extending outside the reagent output portion. The outer diameter of the shunt end portion is the same as the outer diameter of the reagent output portion. A plurality of first flow channels are formed between the outer wall of the shunt vertical portion and the inner wall of the reagent output portion. A plurality of second flow channels are formed between the end surface of the shunt end portion and the end surface of the reagent output portion. The first flow channels communicate with the second flow channels.

[0016] Further, the receiving inner cavity forms a stepped upper space, middle space, and lower space. The upper space is the moving space for the reagent storage portion. The middle space is the deformation space for the elastic component. At least a part of the lower space forms the single-drop storage cavity. A ring-shaped seal is arranged at the bottom opening of the middle space. The lower end of the reagent output portion forms a seal with the ring-shaped seal after passing through the ring-shaped seal. A slender flow channel is provided from the bottom of the lower space to the bottom central position of the dropping container. One end of the slender flow channel communicates with the dropping hole, and the other end outlet forms a reagent outlet.

[0017] Further, the plugging assembly includes a plurality of small balls and a holder. The plurality of small balls are arranged in the holder in a vertically stacked manner. Upper and lower holes are respectively formed in the top and bottom of the holder. The upper hole communicates with the single-drop storage cavity, and the lower hole communicates with the dropping hole. The diameter of each small ball is greater than the diameter of the lower hole, and the diameter of each small ball is less than the inner diameter of the holder. The small ball near the dropping hole has a first position where it communicates with the dropping hole when a negative pressure is generated in the single-drop storage cavity and a second position where it plugs the dropping hole under normal conditions.

[0018] Further, the dropping container further includes an elastic cap-shaped member. The elastic cap-shaped member has a lower part which is a hollow cylinder and an upper part which is a hollow cone with a small hole in the axis. The diameter of the small hole is the same as the diameter of the dropping hole. The cylinder of the elastic cap-shaped member abuts against the top of the holder. The hollow cone is configured to be compressible into the lower hollow cylinder under an external force and automatically return to its original state after the external force is withdrawn so that the reagent output part can move to a termination position where it contacts the top of the hollow cylinder of the elastic cap-shaped member.

[0019] Compared with the prior art, the beneficial effects of the present invention at least include:

[0020] By providing a reagent container and a single-drop storage cavity, the present invention can achieve the function of continuously dropping reagents for a large number of sample experiments by adding the reagent only once. By pushing the reagent container downward through the dropping control assembly, the reagent in the single-drop storage cavity is extruded passively. When no external force is applied to the reagent container, the plugging assembly will keep the dropping hole closed to prevent the reagent from leaking, achieving the effect of passively controlling the dropping of the reagent. By providing a single-drop storage cavity in the dropping container and receiving a quantitative amount of reagent with the single-drop storage cavity, quantitative dropping is achieved. On the one hand, the quality of the experimental results is guaranteed, and on the other hand, the reagent is more saved and the test cost is reduced. By configuring an elastic one-way member at the input end of the reagent output part, the reagent can only flow downward, ensuring that there is no up-and-down backflow of the reagent in the device. At the same time, air can slowly supplement into the reagent container through the elastic one-way member to achieve pressure balance inside and outside the reagent container, and then achieve the continuous dropping function. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic diagram of the experimental reagent continuous quantitative dropping device according to an embodiment of the present invention;

[0022] Figure 2 is Figure 1 a cross-sectional view of the structure;

[0023] Figure 3 is an assembly schematic diagram of the reagent container and the reagent cap according to an embodiment of the present invention;

[0024] Figure 4 is Figure 3 a sectional view of the structure

[0025] Figure 5 is a sectional view of the reagent container according to an embodiment of the present invention;

[0026] Figure 6 is a schematic structural view of the elastic one-way member according to an embodiment of the present invention;

[0027] Figure 7 is a schematic structural view of the flow splitting member according to an embodiment of the present invention;

[0028] Figure 8 is a schematic structural view of the dropping container according to an embodiment of the present invention;

[0029] Figure 9 is Figure 8 a sectional view of the structure

[0030] Figure 10 is a schematic structural view of the plugging assembly according to an embodiment of the present invention;

[0031] Figure 11 is a schematic structural view of the elastic cap-shaped member according to an embodiment of the present invention;

[0032] Figure 12 is a schematic view of the control mechanism according to an embodiment of the present invention;

[0033] Figure 13 is Figure 12 a sectional view of the structure

[0034] Figure 14 is an exploded view of the control mechanism according to an embodiment of the present invention;

[0035] In the figure: 10, reagent container; 11, reagent storage part; 12, reagent output part; 120, limiting step; 121, annular groove; 13, elastic one-way part; 130, lip piece; 14, flow guiding part; 141, flow guiding hole; 142, annular flange; 15, shunt part; 151, shunt vertical part; 152, shunt end part; 153, first flow channel; 154, second flow channel; 16, reagent cap; 20, dropping container; 21, single-drop storage cavity; 22, dropping hole; 23, blocking component; 231, small ball; 232, fixator; 2320, upper hole; 2321, lower hole; 24, elastic component; 241, mounting seat; 242, elastic element; 25, annular seal; 26, elastic cap-shaped part; 261, hollow cylinder; 262, hollow cone; 263, small hole; 27, slender flow channel; 28, protective cover; 30, control mechanism; 31, quantitative control component; 310, pushing part; 3101, mounting part; 3102, pushing part; 3103, graduation line; 311, adjusting part; 32, dropping control component; 321, dropping pressing part; 3210, dropping contact part; 3211, dropping rod part; 322, dropping pressing sleeve; 33, control housing; 331, mounting hole. Detailed implementation manners

[0036] Example embodiments will now be described more fully with reference to the accompanying drawings. However, the example embodiments can be implemented in various forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the example embodiments to those skilled in the art. Like reference numerals refer to like or similar structures in the drawings, and thus their repetitive description will be omitted.

[0037] In the present invention, the words expressing position and direction are described with reference to the drawings as examples, but can be changed as needed, and all changes are included in the protection scope of the present invention.

[0038] As Figures 1 to 14 shown, a continuous quantitative dropping device for experimental reagents provided by the present invention includes:

[0039] A reagent container 10 having a reagent storage part 11 and a reagent output part 12 communicating with the reagent storage part 11. The input end 131 of the reagent output part 12 is configured with an elastic one-way part 13 for controlling the downward flow of the reagent, and its output end is configured with a shunt part 15 for discharging the reagent and allowing air to pass through and be discharged.

[0040] The dropping container 20 has a receiving inner cavity for receiving the reagent container 10. The receiving inner cavity is configured with an elastic component 24 that enables the reagent container 10 to reciprocate in the vertical direction inside it. The receiving inner cavity includes a single-drop storage cavity 21. The single-drop storage cavity 21 has an insertion end and a dropping end through which the reagent output portion 12 slides. A dropping hole 22 communicating with the outside is provided at the dropping end. A blocking component 23 is provided in the single-drop storage cavity 21. The blocking component 23 is configured to keep the dropping hole 22 closed in a non-working state and release the blocking of the dropping hole 22 in a working state.

[0041] The control mechanism 30 includes a dropping control component 32. The dropping control component 32 is used to provide an external force to urge the reagent container 10 to move downward, so as to generate negative pressure in the single-drop storage cavity 21. The blocking component 23 releases the blocking of the dropping hole 22 under the action of the negative pressure, so that the reagent in the single-drop storage cavity 21 flows out from the dropping hole 22.

[0042] In this embodiment, the dropping device includes three independent components: a reagent container 10, a dropping container 20, and a control mechanism 30. Among them, the reagent storage portion 11 of the reagent container 10 is used to store the reagent (liquid) required for the experiment. After the reagent container 10 is filled with the reagent, it is then placed into the receiving inner cavity of the dropping container 20 and the position of the reagent output portion 12 inserted into the single-drop storage cavity 21 is adjusted. Then the dropping test can be carried out. The dropping principle of this device is as follows:

[0043] After the reagent container 10, the dropping container 20 and the control mechanism 30 are assembled, the reagent in the reagent storage portion 11 flows into the single-drop storage cavity 21 through the shunt member 15. Since the blocking assembly 23 is provided in the single-drop storage cavity 21, the blocking assembly 23 keeps the dropping hole 22 closed in the non-working state (when the dropping test is not performed). In this way, the reagent in the single-drop storage cavity 21 will not flow out actively, ensuring that the device does not leak reagent in the non-working state. When reagent dropping is to be performed, the reagent container 10 is driven to move downward (working state) through the dropping control assembly 32, so that the reagent output portion 12 moves in the single-drop storage cavity 21. During the downward movement of the reagent output portion 12, a negative pressure is formed in the single-drop storage cavity 21. Under the action of the negative pressure, the blocking assembly 23 releases the blocking of the dropping hole 22, so that the dropping hole 22 communicates with the single-drop storage cavity 21. In this way, the reagent is allowed to flow out from the dropping hole 22, realizing the passive outflow of the reagent in the reagent container 10. When the external force on the reagent container 10 is removed, the elastic assembly 24 drives the reagent container 10 to move upward to reset. Air is supplemented into the single-drop storage cavity 21 from the dropping hole 22 and air is supplemented into the reagent storage portion 11 from the shunt member 15, so that the internal pressure of the device is restored to balance, ensuring the pressure difference conduction and balance of the device in the working state, and thus realizing the function of continuous reagent dropping; at the same time, during the downward movement of the reagent container 10, a negative pressure is formed in the single-drop storage cavity 21, so that the reagent in the reagent storage portion 11 is continuously supplemented into the single-drop storage cavity 21. Therefore, only by adding the reagent once, the function of continuously dropping the reagent for a large number of sample experiments (until the reagent in the reagent container 10 is exhausted) can be realized. Compared with the traditional method of solving some problems in the experimental tools during pathological experiments, such as too many and complex operation steps, such as using a pipette (two actions of sucking and releasing are required for each addition of reagent), a rubber head dropper / dropping bottle (two actions of sucking and releasing are required for each addition of reagent), the present invention meets the requirements of rapid and quantitative dropping of reagents for a large number of sample experiments (such as pathological immunohistochemical staining experiments, etc.), improving the experimental efficiency and ensuring the experimental quality at the same time.

[0044] In the present invention, the dropping control component 32 pushes the reagent container 10 downward, causing the quantitative reagent in the single-drop storage cavity 21 to be extruded passively through the dropping hole 22 of the device. When no external force is applied to the reagent container 10 (non-operating state), the blocking component 23 closes the dropping hole 22 to prevent reagent leakage. By providing a single-drop storage cavity 21 in the dropping container 20 and using the single-drop storage cavity 21 to receive a quantitative amount of reagent, the present invention can adjust the amount of reagent dropped each time according to the specific requirements of the experiment. By configuring an elastic one-way member 13 at the input end 131 of the reagent output portion 12, the elastic one-way member 13 controls the reagent to flow downward only, thus ensuring that there is no reagent backflow. Since there is no additional pressure compensation design for the reagent container 10, under the action of its elasticity, air can slowly replenish into the reagent container 10 through the elastic one-way member 13, ultimately achieving pressure balance inside and outside the reagent container 10. By configuring a shunt member 15 at the output end of the reagent output portion 12, on the one hand, it realizes the downward shunt discharge of the reagent, and on the other hand, it realizes the upward passage of air into the reagent storage portion 11. Therefore, the combined action of the elastic one-way member 13 and the shunt member 15 ensures that there is no up-and-down convection of the reagent and also ensures that the pressure difference above and below the elastic one-way member 13 can slowly return to balance.

[0045] It can be understood that the reagent container 10 and the dropping container 20 of the present invention can be assembled in a split connection or an integral connection manner. In this embodiment, the reagent container 10 is preferably installed in the receiving inner cavity in a split connection manner. Of course, in other embodiments, the reagent container 10 can also be installed in the receiving inner cavity in a fixed manner. The dropping control component 32 can be in direct contact or indirect contact with the reagent container 10, as long as it can drive the reagent container 10 to move downward. The reagent storage portion 11 of the reagent container 10 can store a set amount of reagent, that is, the reagent storage portion 11 can store the amount of reagent as needed. To ensure no reagent leakage, a reagent cap 16 can be configured at the top of the reagent storage portion 11. To facilitate the removal of the reagent cap 16, the reagent cap 16 can be threadedly connected to the reagent container 10. Further, a sealing ring can be provided inside the reagent cap 16, and the sealing ring fits tightly with the inner wall of the reagent storage portion 11 to prevent reagent from leaking through the threads.

[0046] As a preferred embodiment, the control mechanism 30 further includes a quantitative control component 31, and the quantitative control component 31 is configured to drive the reagent container 10 to move up and down in the receiving inner cavity in a quantifiable manner to quantitatively adjust the different positions of the reagent output portion 12 in the single-drop storage cavity 21, thereby quantitatively adjusting the volume of the single-drop storage cavity 21.

[0047] In this embodiment, in order to achieve precise control of the single-drop dosage, the volume of the single-drop storage chamber 21 is quantitatively adjusted, so that the amount of reagent received by the single-drop storage chamber 21 can be precisely adjusted, thereby achieving precise control of the single-drop dosage. That is, the present invention can adjust the amount of reagent dropped per single drop, realizing the function of quantitative reagent dropping during the experiment. On the one hand, the quality of the experimental results is ensured, and on the other hand, reagents are more saved. It can be understood that the present invention can adjust the liquid output volume of the single-drop addition through the quantitative control component 31 according to the specific requirements of the experiment, with higher flexibility; at the same time, the quantitative control component 31 controls the reagent container 10 to change its position in a quantifiable manner, replacing the method of relying on human eye observation, thereby quantifying the adjustment displacement of the reagent container 10 and eliminating the errors generated by the artificial judgment method, with better adjustment accuracy.

[0048] As a preferred embodiment, the quantitative control component 31 includes a pusher 310 and an adjuster 311 for controlling the quantifiable movement of the pusher 310 in the up and down directions. The pusher 310 is arranged above the top of the reagent container 10 in a manner that it can move in the up and down directions.

[0049] In this embodiment, in order to further improve the convenience of operation, the quantitative control component 31 of the present invention controls the up and down movement of the pusher 310 through the adjuster 311, and the pusher 310 pushes the reagent container 10 to move up and down in the receiving inner cavity, making the position adjustment of the reagent output part 12 more precise. At the same time, the adjuster 311 can make the movement amount of the pusher 310 quantifiable, which is not only quantitatively accurate but also can quickly adjust the single-drop dosage as needed. Therefore, the quantitative control component 31 of the present invention realizes the flexible adjustment of the single-drop reagent dosage within the range of the measuring range.

[0050] As a preferred embodiment, the dropping control component 32 includes a dropping pressure member 321. The dropping pressure member 321 is arranged above the reagent container 10 in a manner that it can move in the up and down directions, and when receiving a downward pressing force, it transmits the pressing force to the reagent container 10.

[0051] In this embodiment, in order to simplify the dropping process, the reagent is dropped by pressing downward. During dropping, the operator only needs to press the dropping pressure member 321 with one hand to drive the reagent container 10 to move downward, realizing the outflow of the reagent. After releasing the dropping pressure member 321, the reagent container 10 can quickly reset under the action of the elastic component 24. By pressing and releasing once, the dropping and replenishment of the reagent can be realized, greatly simplifying the operation steps of the dropping experiment, and the operation steps are simpler. It can be understood that each time the dropping pressure member 321 is pressed, the reagent can be added to one experimental group, and continuous dropping can be carried out until the reagent in the reagent container 10 is exhausted.

[0052] Of course, in other embodiments, the dropping control component 32 may also drive the reagent container 10 to move downward in other structures, such as driving the reagent container 10 to move downward by mechanical means.

[0053] As a preferred embodiment, the control mechanism 30 further includes a control housing 33 detachably coupled to the upper portion of the dropping container 20. The quantitative control component 31 and the dropping control component 32 are integrated on the control housing 33. The upper portion of the reagent container 10 is received inside the control housing 33. An installation hole 331 is formed in the upper end surface of the control housing 33. The pusher 310 has an installation portion 3101 threadedly engaged with the installation hole 331. A pushing portion 3102 located inside the control housing 33 is provided at the bottom end of the installation portion 3101. The top end of the installation portion 3101 is threadedly connected to the adjusting member 311. Scale lines 3103 indicating its movement amount are shown on the outer wall of the installation portion 3101. The dropping pressure member 321 includes a dropping contact portion 3210 located inside the control housing 33 and below the pushing portion 3102, and a dropping rod portion 3211 vertically protruding from the upper surface of the dropping contact portion 3210. The dropping rod portion 3211 extends outside the control housing 33 after passing through the pushing portion 3102, the installation portion 3101, and the adjusting member 311 in sequence.

[0054] In this embodiment, in order to further simplify the control of the dropping device, the quantitative control component 31 and the dropping control component 32 are integrated on a control housing 33, so that the quantitative adjustment and dropping operations of the device can be more convenient. Among them, the control housing 33 is detachably combined with the upper part of the dropping container 20, so that the reagent container 10 is completely wrapped by the inner cavity of the control housing 33 and the receiving inner cavity of the dropping container 20 to prevent the reagent container 10 from being damaged. Preferably, the inner wall of the lower part of the control housing 33 is provided with an internal thread, and the outer wall of the upper part of the dropping container 20 is provided with an external thread adapted to the internal thread of the control housing 33, so that the control housing 33 and the dropping container 20 can be assembled together by means of threaded connection, and the assembly is simpler. In addition, both the quantitative control component 31 and the dropping control component 32 are detachably mounted on the control housing 33, which is convenient for disassembling and cleaning each component, and can be reused repeatedly, greatly saving costs. Preferably, the installation methods of the quantitative control component 31 and the dropping control component 32 are as follows: the pushing member 310 is connected to the mounting hole 331 of the control housing 33 by means of threaded connection, and the adjusting member 311 is connected to the pushing member 310 by means of threaded connection. Thus, by rotating the adjusting member 311, the pushing part 3102 of the pushing member 310 can be moved in the up and down direction. Since the pushing part 3102 is located above the dropping contact part 3210, the pushing part 3102 will drive the dropping contact part 3210 to move downward, and then the dropping contact part 3210 will push the reagent container 10 to move downward. Among them, the quantifiable adjustment principle of the quantitative control component 31 is: since the bottom of the reagent container 10 is elastically supported by the elastic component 24, the initial position of the pushing part 3102 determines the initial compression amount of the elastic component 24, corresponding to the single liquid discharge amount. Therefore, by rotating the adjusting member 311 to adjust the position of the pushing part 3102, the liquid discharge amount of a single press can be quantitatively adjusted. Among them, when the adjusting member 311 is screwed to the lowest position (adhering to the top surface of the control housing 33), the pushing part 3102 also reaches its lowest position. At this time, the bottom of the shunt member 15 of the reagent container 10 part has contacted the bottom of the single-drop storage cavity 21. Since there is no movable space, the dropping pressure member 321 cannot be pressed at this time, and the reagent dropping amount is zero. When the adjusting member 311 is screwed to the highest position (the pushing part 3102 is closely attached to the inner end surface of the control housing 33), the bottom of the shunt member 15 in the reagent container 10 is aligned with the insertion end of the single-drop storage cavity 21, and the single reagent dropping amount is the largest. Therefore, by rotating the adjusting member 311, the pushing part 3102 can be moved up and down, thereby adjusting the dropping amount of a single reagent; at the same time, by displaying a scale line 3103 of its moving amount on the outer wall of the mounting part 3101 of the pushing member 310, the actual amount of a single drop can be quantified, the adjustment accuracy is higher, and the adjustment method is simplified.

[0055] In addition, in this embodiment, in order to facilitate pressing the dropping pressure member 321, a dropping pressure sleeve 322 is provided at the top of the dropping rod portion 3211. The dropping pressure sleeve 322 is assembled with the dropping rod portion 3211 in a threaded manner. In this way, by pressing the dropping pressure sleeve 322, the downward movement of the dropping contact portion 3210 can be realized, and then the reagent container 10 is driven to move downward, making the operation more comfortable.

[0056] Among them, the installation sequence of the above-mentioned pushing member 310, adjusting member 311, dropping pressure member 321 and dropping pressure sleeve 322 is as follows: first, install the pushing member 310 on the control housing 33, then install the adjusting member 311 on the pushing member 310, and then pass the dropping rod portion 3211 of the dropping pressure member 321 through the pushing member 310 and the adjusting member 311 in sequence, and finally install the dropping pressure sleeve 322 at the top of the dropping rod portion 3211.

[0057] As a preferred embodiment, the receiving inner cavity is formed with a stepped upper space, middle space and lower space. The upper space is the moving space for the reagent storage portion 11 to move. The middle space is the deformation space of the elastic component 24. At least part of the lower space forms the single-drop storage cavity 21. A ring-shaped seal 25 is arranged at the bottom opening of the middle space. The lower end of the reagent output portion 12 forms a seal with the ring-shaped seal 25 after passing through the ring-shaped seal 25. A slender flow channel 27 is provided from the bottom of the lower space to the bottom axis position of the dropping container 20. One end of the slender flow channel 27 is communicated with the dropping hole 22, and the other end outlet forms a reagent outlet.

[0058] In this embodiment, by providing an upper space, a middle space and a lower space in the receiving inner cavity, each layer of space plays a positioning role in the installation of the corresponding components, making the installation of each internal component more convenient. Among them, the upper space forms a step for the bottom surface of the reagent storage portion 11 to abut against. A ring-shaped groove 121 is provided at the bottom of the middle space. The ring-shaped groove 121 is used to install the ring-shaped seal 25. The setting of the ring-shaped seal 25 can ensure that the reagent output portion 12 forms a seal with it after passing through the ring-shaped seal 25, preventing the reverse flow of the reagent. Preferably, the elastic component 24 of the present invention includes an elastic element and a mounting seat 241. The elastic element 242 is preferably a spring. The spring and the mounting seat 241 are both installed in the middle space. During installation, first install the mounting seat 241 at the bottom of the middle space, and then put the spring on the mounting seat 241. After the reagent container 10 is placed in the receiving inner cavity, the bottom of the reagent storage portion 11 contacts the spring, and the reagent container 10 reciprocates up and down in the receiving inner cavity through the spring.

[0059] In addition, the drip hole 22 communicates with the slender flow channel 27. The slender flow channel 27 can generate a capillary effect to prevent the reagent from leaking out under gravity by using capillary force. Only under an external force (the applied external force > capillary force), the reagent can be pushed out of the device. It can be understood that the dropping container 20 can be configured with a cover 28 to protect the reagent outlet to prevent the reagent outlet from being contaminated.

[0060] As a preferred embodiment, the plugging assembly 23 includes a plurality of small balls 231 and a holder 232. The plurality of small balls 231 are arranged in the holder 232 in a vertically stacked manner. The top and bottom of the holder 232 are respectively provided with an upper hole 2320 and a lower hole 2321. The upper hole 2320 communicates with the single-drop storage cavity 21, and the lower hole 2321 communicates with the drip hole 22. The diameter of each small ball 231 is greater than the diameter of the lower hole 2321, and the diameter of each small ball 231 is less than the inner diameter of the holder 232. The small ball 231 close to the drip hole 22 has a first position for communicating with the drip hole 22 when a negative pressure is generated in the single-drop storage cavity 21 and a second position for normally plugging the drip hole 22.

[0061] In this embodiment, the working principle of the plugging assembly 23 is as follows: When the drip pressing member 321 is not pressed, each small ball 231 plugs the drip hole 22 under the action of gravity and the surface tension of the reagent in the single-drop storage cavity 21, which can prevent the reagent from oozing downward under gravity. When the drip pressing member 321 is pressed, the reagent pressure suddenly increases and is significantly greater than the gravity of the small balls 231, and the reagent will push up the small balls 231 (away from the drip hole 22), and the reagent can flow out. In addition, since a plurality of small balls 231 are installed in the holder 232 and the diameter of the small balls 231 is slightly smaller than the inner diameter of the holder 232, the reagent flowing into the holder 232 can only flow downward through the gap between the inner wall of the holder 232. Since the contact area between the small balls 231 and the inner wall of the holder 232 is very narrow and thin, it is easy to generate a capillary effect to avoid the slow leakage of the reagent under gravity. Therefore, by adopting the design of multiple small balls 231, the two methods of capillary force plugging and gravity plugging can be used simultaneously for this device to prevent the reagent from leaking out under gravity (without other external forces), and the leak-proof performance is better.

[0062] As a preferred embodiment, the dropping container 20 further includes an elastic cap-shaped member 26. The elastic cap-shaped member 26 has a lower hollow cylinder 261 and an upper hollow cone 262 with a small hole 263 in the axis. The diameter of the small hole 263 is the same as the diameter of the dropping hole 22. The cylinder of the elastic cap-shaped member 26 abuts against the top of the holder 232. The hollow cone 262 is configured to be compressed into the lower hollow cylinder under an external force and automatically return to its original state after the external force is withdrawn, so that the reagent output portion 12 can move to the end position in contact with the top of the hollow cylinder 261 of the elastic cap-shaped member 26.

[0063] In this embodiment, by providing the elastic cap-shaped member 26 in the single-drop storage chamber 21, since the hollow cone 262 of the elastic cap-shaped member 26 is configured to be compressed into the lower hollow cylinder under an external force and automatically return to its original state after the external force is withdrawn, the upper limit value of the reagent dropping amount can be adjusted by deforming the elastic cap-shaped member 26. That is, after the reagent container 10 is placed in the receiving inner cavity, in the initial state (after the device is installed), the bottom of the shunt member 15 of the reagent container 10 is aligned with the bottom of the middle layer space. At this time, the maximum amount of the single-drop reagent is the volume between the top of the lower layer space and the elastic cap-shaped member 26, and this volume is the upper limit value of the reagent dropping amount. When the reagent container 10 moves downward and the bottom of the shunt member 15 of the reagent output portion 12 contacts the top of the hollow cylinder 261 of the elastic cap-shaped member 26, since there is no movable space, the dropping pressure member 321 cannot be pressed any more at this time, and the reagent dropping amount is zero. Therefore, the elastic cap-shaped member 26 also plays a positioning role in completely discharging the reagent in the single-drop storage chamber 21.

[0064] As a preferred embodiment, a flow guide member 14 and an elastic one-way member 13 are sequentially arranged from top to bottom at one end of the reagent output portion 12. The output end of the elastic one-way member 13 is provided with a lip 130 that maintains its normally closed state by elasticity. The flow guide member 14 is provided with a flow guide hole 141. One end of the flow guide hole 141 is communicated with the reagent storage portion 11, and the other end is communicated with the input end of the elastic one-way member 13.

[0065] In this embodiment, the flow guide member 14 and the elastic one-way member 13 are combined to form the flow guide unit of the reagent container 10. The function of the elastic one-way member 13 is to make the reagent in the reagent container 10 flow unidirectionally from top to bottom. In order to keep the elastic one-way member 13 stationary in the reagent output part 12, a flow guide member 14 is arranged above the elastic one-way member 13. A flow guide hole 141 is provided in the middle of the flow guide member 14. The lower part of the flow guide member 14 is embedded in the top of the elastic one-way member 13. The reagent in the reagent storage part 11 flows through the flow guide hole 141 to the elastic one-way member 13, and the elastic one-way member 13 controls the unidirectional flow of the reagent. Then the reagent enters the single-drop storage cavity 21 through the flow splitting member 15. Preferably, the elastic one-way member 13 in this embodiment is made of rubber material, which has flexible extensibility and shrinkability, enabling the reagent in the reagent container 10 to pass through under a small pressure difference condition, and being able to restore deformation to prevent the reagent from flowing out when the upper and lower pressures are basically balanced. Moreover, since there is no additional pressure supplement in the reagent container 10, the air outside the reagent container 10 can be slowly supplemented into the reagent container 10 through the elastic one-way member 13, ultimately achieving pressure balance inside and outside the reagent container 10. Therefore, the elastic one-way member 13 plays a key role in realizing the unidirectional downward outflow of the reagent and the upward supplement of air.

[0066] In addition, in addition to the flow guiding function, the flow guide member 14 in this embodiment also plays a role in fixing the elastic one-way member 13. Among them, an annular flange 142 is provided at the top edge of the flow guide member 14, and an annular groove 121 adapted to the annular flange 142 is provided in the reagent output part 12. The annular flange 142 of the flow guide member 14 is embedded in the annular groove 121, which can ensure its position is fixed, and then limit the top of the elastic one-way member 13, while the bottom of the elastic one-way member 13 is limited by the limit step 120 in the reagent output part 12.

[0067] As a preferred implementation manner, the flow splitting member 15 includes a flow splitting vertical part 151 inserted into the reagent output part 12 and a flow splitting end part 152 extending outside the reagent output part 12. The outer diameter of the flow splitting end part 152 is the same as the outer diameter of the reagent output part 12. A plurality of first flow channels 153 are formed between the outer wall of the flow splitting vertical part 151 and the inner wall of the reagent output part 12, and a plurality of second flow channels 154 are formed between the end face of the flow splitting end part 152 and the end face of the reagent output part 12. The first flow channels 153 are communicated with the second flow channels 154.

[0068] In this embodiment, since the flow splitter 15 is used for reagent diversion and discharge and for air to enter upward, by providing a plurality of first flow channels 153 and second flow channels 154, some of the flow channels are occupied by the reagent flowing downward, and some of the flow channels are occupied by the air entering upward. In this way, the downward outflow of the reagent and the upward entry of the air can be carried out simultaneously, achieving a gradual balance of the pressure difference. In addition, the flow splitter 15 also serves to subdivide the reagent channels, making the reagent outflow path more complex and achieving the purpose of controlling the reagent flow rate and flow volume.

[0069] In summary, the dropping device of the present invention simplifies the reagent dropping steps of the previous "extraction → transfer → release of reagent" (taking a pipette as an example) to the extent of "completing the reagent addition with a single press". It has accurate quantification and can adjust the single-drop amount as needed. The operation is simple and convenient, and it is especially suitable for rapid quantitative addition of a large number of experimental reagents. Compared with traditional droppers, it has the following advantages:

[0070] (1) The present invention can achieve the function of continuously dropping reagents for a large number of sample experiments (until the reagents in the reagent container 10 are exhausted) by adding the reagent only once, solving the problems of complex operation process and many steps in the experimental tools in previous pathological experiments. For example, when using a pipette (two actions of extraction and release have to be completed for each addition of reagent), a rubber head dropper / dropping bottle (two actions of extraction and release have to be completed for each addition of reagent).

[0071] (2) The present invention can adjust the single-drop amount of the reagent, achieving the function of quantitative reagent dropping in the experimental process. On the one hand, it ensures the quality of the experimental results, and on the other hand, it saves more reagents and reduces the experimental cost.

[0072] (3) The device of the present invention is small in size, simple in operation, and has obvious practicability and portability.

[0073] (4) Each component of the present invention is detachable and can be cleaned, with simple installation, can be reused repeatedly, and can greatly save costs.

[0074] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Without departing from the principle and purpose of the present invention, those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the invention, and all these changes should fall within the protection scope of the claims of the present invention.

Claims

1. An experimental reagent continuous quantitative dropping device, characterized in that, include: A reagent container (10) comprising a reagent storage portion (11) and a reagent output portion (12) connected to the reagent storage portion (11), wherein an input end (131) of the reagent output portion (12) is provided with an elastic one-way member (13) for controlling the downward outflow of the reagent, and an output end thereof is provided with a flow diverter (15) for diverting and discharging the reagent and for allowing air to flow upward; A dropping container (20), comprising an inner cavity for accommodating the reagent container (10), the inner cavity being provided with an elastic component (24) for enabling the reagent container (10) to reciprocate in the vertical direction therein, the inner cavity comprising a single dropping storage cavity (21), the single dropping storage cavity (21) having an insertion end and a dropping end for the reagent output portion (12) to slide through, the dropping end being provided with a dropping hole (22) communicating with the outside, the single dropping storage cavity (21) being provided with a blocking component (23), the blocking component (23) being configured to keep the dropping hole (22) closed in a non-working state and to release the blocking of the dropping hole (22) in a working state; The control mechanism (30) comprises a dripping control component (32), wherein the dripping control component (32) is used to provide an external force for causing the reagent container (10) to move downward, so that the single dripping storage chamber (21) generates a negative pressure, and the blocking component (23) releases the blockage of the dripping hole (22) under the action of the negative pressure, so that the reagent in the single dripping storage chamber (21) flows out from the dripping hole (22).

2. The continuous quantitative dropping device for experimental reagents according to claim 1, wherein The control mechanism (30) further comprises a quantitative control component (31), wherein the quantitative control component (31) is configured to drive the reagent container (10) to move up and down in the receiving cavity in a quantifiable manner, so as to quantitatively adjust different positions of the reagent output portion (12) in the single-drop storage cavity (21), thereby quantitatively adjusting the volume of the single-drop storage cavity (21).

3. The continuous quantitative dropping device for experimental reagents according to claim 2, wherein The quantitative control component (31) comprises a pusher (310) and an adjusting member (311) for controlling the quantitative movement of the pusher (310) in the up and down directions. The pusher (310) is arranged above the top of the reagent container (10) in a manner that it can move in the up and down directions.

4. The continuous quantitative dropping device for experimental reagents according to claim 3, characterized in that, The dripping control component (32) includes a dripping pressure piece (321) which is arranged above the reagent container (10) in a manner that allows it to move in an up-down direction and transmits the operating force to the reagent container (10) when receiving a downward pressing operating force.

5. The continuous quantitative dropping device for experimental reagents according to claim 4, characterized in that, The control mechanism (30) further includes a control housing (33) detachably coupled to the upper part of the dropping container (20). The metering control assembly (31) and the dropping control assembly (32) are integrated on the control housing (33). The upper part of the reagent container (10) is received inside the control housing (33). An installation hole (331) is formed in the upper end face of the control housing (33). The pusher (310) has an installation portion (3101) threadedly engaged with the installation hole (331). A pushing portion (3102) located inside the control housing (33) is provided at the bottom end of the installation portion (3101). The top end of the installation portion (3101) is threadedly connected to the adjusting member (311). Scale lines (3103) indicating its movement amount are shown on the outer wall of the installation portion (3101). The dropping pressing member (321) includes a dropping contact portion (3210) located inside the control housing (33) and below the pushing portion (3102), and a dropping rod portion (3211) vertically protruding from the upper surface of the dropping contact portion (3210). The dropping rod portion (3211) extends outside the control housing (33) after passing through the pushing portion (3102), the installation portion (3101), and the adjusting member (311) in sequence.

6. The continuous quantitative dropping device for experimental reagents according to claim 1, wherein A flow guiding member (14) and an elastic one-way member (13) are sequentially arranged from top to bottom at one end of the reagent output portion (12). A lip piece (130) that maintains its normally closed state by elasticity is provided at the output end of the elastic one-way member (13). The flow guiding member (14) is provided with a flow guiding hole (141). One end of the flow guiding hole (141) communicates with the reagent storage portion (11), and the other end communicates with the input end (131) of the elastic one-way member (13).

7. The continuous quantitative dropping device for experimental reagents according to claim 1, characterized in that, The shunt member (15) includes a shunt vertical portion (151) inserted into the reagent output portion (12) and a shunt end portion (152) extending outside the reagent output portion (12). The outer diameter of the shunt end portion (152) is the same as the outer diameter of the reagent output portion (12). A plurality of first flow channels (153) are formed between the outer wall of the shunt vertical portion (151) and the inner wall of the reagent output portion (12). A plurality of second flow channels (154) are formed between the end face of the shunt end portion (152) and the end face of the reagent output portion (12). The first flow channels (153) communicate with the second flow channels (154).

8. The continuous quantitative dropping device for experimental reagents according to claim 1, characterized in that The accommodating inner cavity forms a stepped upper space, middle space, and lower space. The upper space is the moving space for the reagent storage part (11). The middle space is the deformation space for the elastic component (24). At least part of the lower space forms the single-drop storage cavity (21). At the bottom opening of the middle space, an annular seal (25) is arranged. The lower end of the reagent output part (12) forms a seal with the annular seal (25) after passing through the annular seal (25). From the bottom of the lower space to the bottom axial center position of the dropping container (20), a slender flow channel (27) is provided. One end of the slender flow channel (27) is communicated with the dropping hole (22), and the other end outlet forms a reagent outlet.

9. The continuous quantitative dropping device for experimental reagents according to claim 8, characterized in that, The blocking component (23) includes a plurality of small balls (231) and a holder (232). The plurality of small balls (231) are arranged in the holder (232) in a vertically stacked manner. Upper holes (2320) and lower holes (2321) are respectively formed at the top and bottom of the holder (232). The upper holes (2320) are communicated with the single-drop storage cavity (21), and the lower holes (2321) are communicated with the dropping holes (22). The diameter of each small ball (231) is larger than the diameter of the lower hole (2321), and the diameter of each small ball (231) is smaller than the inner diameter of the holder (232). The small ball (231) close to the dropping hole (22) has a first position for communicating with the dropping hole (22) when negative pressure is generated in the single-drop storage cavity (21) and a second position for normally blocking the dropping hole (22).

10. The continuous quantitative dropping device for experimental reagents according to claim 9, characterized in that, The dropping container (20) further includes an elastic cap-shaped member (26). The elastic cap-shaped member (26) has a lower part that is a hollow cylinder (261) and an upper part that is a hollow cone (262) with a small hole (263) at the axis. The diameter of the small hole (263) is the same as the diameter of the dropping hole (22). The cylinder of the elastic cap-shaped member (26) abuts against the top of the holder (232). The hollow cone (262) is configured to be compressible into the lower hollow cylinder under an external force and automatically return to its original state after the external force is withdrawn so that the reagent output part (12) can move to a termination position in contact with the top of the hollow cylinder (261) of the elastic cap-shaped member (26).

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