Vacuum pipettor for somatic immunotherapy

Abstract

The invention relates to the technical field of medical instruments, and discloses a somatic immunotherapy vacuum pipettor which comprises a piston type pipetting mechanism and a threaded quantity control mechanism, the first external threaded rod and the second external threaded rod generate reverse movement when the threaded structure is stressed, and the built-in limiting ring is placed in the transparent liquid suction pipe and can generate height limiting on upward movement of the built-in piston body. According to the somatic immunotherapy vacuum pipettor, the maximum amount of sucked liquid at a single time can be accurately controlled by controlling the maximum movement amount of the built-in piston body in the liquid suction process, and the accurate discharge amount of the liquid at a single time within the range of the maximum suction amount at a single time can be controlled through relative rotation of the thread structure in the liquid discharge process, so that the accuracy of the liquid suction amount at a single time is improved. And therefore, the control effect of the equipment on the liquid quantity precision in the liquid transfer process is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly to a somatic cell immunotherapy vacuum pipette. Background Art

[0002] During the somatic cell immunotherapy process, precise transfer and aliquoting of cell suspensions are required. Therefore, a pipette is needed, and a pipette, also known as a micropipette, is a device used for quantitatively transferring liquids.

[0003] For example, Chinese Patent Publication No. "CN111408423B" discloses a "pipette for use with a pipette tip", the main structure of which includes a rod-shaped pipette housing; a pin on the lower end of the pipette housing for holding the pipette tip; a driving device for moving a displacement element to suck a liquid sample into the pipette tip held on the pin and to discharge the liquid sample from the pipette tip; a unloading device including a curved bracket rotatably supported about a longitudinal axis in the pipette housing, a first contact element on an unloading rod guided on a first curve on the circumference of the curved bracket, and an operating element connected to the curved bracket and extending out of the pipette housing and rotatable relative to the pipette housing, the unloading rod being guided in the pipette housing so as to be movable in the longitudinal direction of the pin, and the unloading device being configured to rotate the curved bracket by rotating the operating element from an initial position, and the first curve causing the first contact element to move downward so that the unloading rod presses the pipette tip held on the pin off the pin.

[0004] However, the above pipette cannot effectively control the accuracy of liquid suction and discharge. During the somatic cell immunotherapy process, precise transfer and aliquoting of cell suspensions are required, resulting in poor control accuracy. Workers can only observe the amount of liquid discharged or sucked by observing the scale on the surface of the pipette tube. And when the amount of liquid sucked or discharged reaches the requirement, it is necessary to manually pause the internal piston. Since there is no corresponding auxiliary component for hand control, it is likely to occur that the amount of liquid sucked or discharged is too much or too little. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the present invention provides a somatic cell immunotherapy vacuum pipette, which can precisely control the maximum amount of liquid sucked in a single time by controlling the maximum movement amount of the built-in piston body during the liquid suction process. During the liquid discharge process, through the relative rotation of the threaded structure, it can control the precise discharge amount of liquid in a single time within the range of the maximum amount of liquid sucked in a single time, thereby improving the control effect of the equipment on the liquid volume accuracy during the liquid transfer process and solving the above technical problems.

[0006] To achieve the above object, the present invention provides the following technical solution: A somatic cell immunotherapy vacuum pipette, comprising a pipette tube capable of sucking in liquid and storing the liquid inside it, and further comprising a piston-type pipetting mechanism, which is internally provided with a transparent liquid suction tube having a hollow structure and being transparent, a built-in piston body placed inside the transparent liquid suction tube and capable of moving axially along the transparent liquid suction tube to change the air pressure inside the pipette tube, and a capacity scale value provided on the outer circumferential surface of the transparent liquid suction tube and capable of reflecting the volume of liquid entering the pipette tube; and a threaded metering mechanism, which is internally provided with a first external threaded rod and a second external threaded rod that move in the reverse direction when subjected to the threaded structure, and a built-in limit ring placed inside the transparent liquid suction tube and capable of imposing a height limit on the upward movement of the built-in piston body.

[0007] Preferably, the piston-type pipetting mechanism further comprises a helical spring. The inside of the transparent liquid suction tube is provided with a piston moving cavity with an open bottom end. The transparent liquid suction tube is provided with a pipe card entrance at the bottom opening end of the piston moving cavity, which can be snapped into the top plug of the pipette tube. The outer circumferential surface of the transparent liquid suction tube is provided with longitudinally distributed capacity scale values. A first rod body perforation is provided at the center of the top end of the transparent liquid suction tube. A second rod body perforation is provided in the structure near the edge of the top of the transparent liquid suction tube. A built-in piston body capable of moving axially along the piston moving cavity is placed inside the piston moving cavity of the transparent liquid suction tube. A longitudinal pull rod penetrating the first rod body perforation is fixedly installed at the top end of the built-in piston body. A top force application plate is fixedly installed at the top end of the longitudinal pull rod. A third rod body perforation longitudinally corresponding to the second rod body perforation is provided in the plate body of the top force application plate. A helical spring in a compressed state is sleeved around the rod body of the longitudinal pull rod between the transparent liquid suction tube and the top force application plate.

[0008] Preferably, the transparent liquid suction tube is made of transparent PVC material.

[0009] Preferably, the sizes of the pipe card entrance and the top plug of the pipette tube that it is snapped into match the sizes of the two end joints of the corresponding sterile filter.

[0010] Preferably, the value on the surface of the capacity scale value corresponding to the bottom port of the built-in piston body is the amount of liquid entering the pipette tube.

[0011] Preferably, the threaded metering mechanism further includes a threaded sleeve. One end of the threaded sleeve is provided with a first internal thread cavity with an inward concave structure, and the other end of the threaded sleeve is provided with a second internal thread cavity with an inward concave structure. The rod body of the first external threaded rod is installed inside the first internal thread cavity through a first thread structure, and the rod body of the second external threaded rod is installed inside the second internal thread cavity through a second thread structure. The opposite ends of the first external threaded rod and the second external threaded rod are provided with a polygonal limiting cavity with an inward concave structure. A polygonal limiting rod inserted into the polygonal limiting cavity is fixedly installed at the center of the threaded sleeve. One end of the first external threaded rod is provided with a first connecting plate integrally formed therewith, and one end of the second external threaded rod is provided with a second connecting plate integrally formed therewith. A fixed collar fixed to the bottom area of the transparent liquid suction tube is installed at the bottom end of the first connecting plate. A top connecting plate is fixedly installed at the top end of the second connecting plate. A longitudinal limiting rod passing through the third rod body perforation and the second rod body perforation is fixedly installed on the bottom surface of the top connecting plate. An internal limiting ring capable of axially moving along the piston moving cavity is fixedly installed at the bottom end of the longitudinal limiting rod inside the piston moving cavity. A rod body moving perforation for the longitudinal pull rod to move is provided at the center of the internal limiting ring.

[0012] Preferably, the cross-sectional structure of the polygonal limiting cavity is the same as that of the polygonal limiting rod, both are polygonal structures, and the cross-sectional dimension of the polygonal limiting cavity matches the cross-sectional dimension of the polygonal limiting rod.

[0013] Preferably, the first thread structure includes an internal thread structure provided inside the first internal thread cavity and an external thread structure provided on the rod body of the first external threaded rod. The second thread structure includes an internal thread structure provided inside the second internal thread cavity and an external thread structure provided on the rod body of the second external threaded rod, and the spiral direction of the first thread structure is opposite to that of the second thread structure.

[0014] Compared with the prior art, the present invention provides a somatic cell immunotherapy vacuum pipette, which has the following beneficial effects: During the liquid suction process, by controlling the maximum movement amount of the internal piston body, the maximum single amount of liquid sucked can be accurately controlled. During the liquid discharge process, through the relative rotation of the thread structure, the accurate single liquid discharge amount can be controlled within the range of the maximum single suction amount, thereby improving the control effect of the equipment on the liquid volume accuracy during the liquid transfer process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A perspective view of the present invention after being subjected to a downward pressing force; Figure 2A three-dimensional sectional view of the present invention after being subjected to a downward pressing force; Figure 3 A three-dimensional view of the piston-type pipetting mechanism of the present invention after being subjected to a downward pressing force; Figure 4 A three-dimensional sectional view of the piston-type pipetting mechanism of the present invention after being subjected to a downward pressing force; Figure 5 A three-dimensional view of the screw-type metering mechanism of the present invention; Figure 6 A three-dimensional sectional view of the screw-type metering mechanism of the present invention.

[0016] Wherein: 1. Pipette; 2. Piston-type pipetting mechanism; 21. Transparent liquid suction tube; 22. Volume scale value; 23. Piston moving cavity; 24. Pipe card inlet; 25. First rod body perforation; 26. Second rod body perforation; 27. Built-in piston body; 28. Longitudinal pull rod; 29. Top force application plate; 210. Helical spring; 211. Third rod body perforation; 3. Screw-type metering mechanism; 31. Threaded sleeve; 32. First internal thread cavity; 33. Second internal thread cavity; 34. First thread structure; 35. Second thread structure; 36. First external threaded rod; 37. Second external threaded rod; 38. First connecting plate; 39. Second connecting plate; 310. Polygonal limiting cavity; 311. Polygonal limiting rod; 312. Fixed collar; 313. Top connecting plate; 314. Longitudinal limiting rod; 315. Built-in limiting ring; 316. Rod body movable perforation. Detailed implementation manners

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0018] Please refer to Figure 1 and Figure 2 , a somatic cell immunotherapy vacuum pipette, including a pipette 1 capable of sucking liquid and storing the liquid inside it. The top plug of the pipette 1 can be inserted into a sterile filter, and then the plug of the sterile filter can be inserted into the pipe card inlet 24, which can effectively prevent contamination and ensure the sterility of the cell culture and processing process.

[0019] In order to achieve the vacuum suction function of somatic cell liquid, please refer to Figure 1 , Figure 2 , Figure 3 and Figure 4, it is necessary to set up a piston-type pipetting mechanism 2, which is internally provided with a transparent liquid suction pipe 21 with a hollow and transparent structure, a built-in piston body 27 placed inside the transparent liquid suction pipe 21 and capable of moving along the axial direction of the transparent liquid suction pipe 21 to change the air pressure inside the pipette 1, and a capacity scale value 22 set on the outer circumferential surface of the transparent liquid suction pipe 21 and capable of reflecting the volume of liquid entering the pipette 1. Press down the top force-applying plate 29 until the built-in piston body 27 moves to the lowest point of the piston moving cavity 23, then insert the bottom suction port of the pipette 1 below the liquid level of the somatic cell liquid, and release the top force-applying plate 29. Under the elastic action of the helical spring 210, the top force-applying plate 29 will drive the built-in piston body 27 to reset upward. At this time, the somatic cell liquid will be sucked into the pipette 1 until the built-in piston body 27 no longer moves upward, and a quantitative somatic cell liquid can be sucked into the pipette 1 and stored in advance.

[0020] For the specific structure of the piston-type pipetting mechanism 2, please refer to Figure 3 and Figure 4 , and it further includes a helical spring 210. The inside of the transparent liquid suction pipe 21 is provided with a piston moving cavity 23 with an open bottom end. The transparent liquid suction pipe 21 is provided with a pipe card entrance 24 at the bottom opening end of the piston moving cavity 23, which can be inserted into the top plug of the pipette 1. The outer circumferential surface of the transparent liquid suction pipe 21 is provided with longitudinally distributed capacity scale values 22. The center of the top end of the transparent liquid suction pipe 21 is provided with a first rod body perforation 25. The top of the transparent liquid suction pipe 21 is provided with a second rod body perforation 26 in the structure near its edge. The transparent liquid suction pipe 21 places a built-in piston body 27 inside the piston moving cavity 23, which can move axially along the piston moving cavity 23. The top end of the built-in piston body 27 is fixedly installed with a longitudinal pull rod 28 passing through the first rod body perforation 25. The top end of the longitudinal pull rod 28 is fixedly installed with a top force-applying plate 29. The plate body of the top force-applying plate 29 is provided with a third rod body perforation 211 longitudinally corresponding to the second rod body perforation 26. A helical spring 210 in a compressed state is sleeved outside the rod body of the longitudinal pull rod 28 between the transparent liquid suction pipe 21 and the top force-applying plate 29. The transparent liquid suction pipe 21 is made of transparent PVC material. The sizes of the pipe card entrance 24 and the top plug of the pipette 1 are matched with the two end joints of the corresponding sterile filter. The value on the surface of the capacity scale value 22 corresponding to the bottom port of the built-in piston body 27 is the amount of liquid entering the pipette 1.

[0021] In order to achieve the precise control effect of the liquid volume, please refer to Figure 1 , Figure 2 , Figure 5 and Figure 6, it is necessary to set a threaded metering mechanism 3, which is internally provided with a first external threaded rod 36 and a second external threaded rod 37 that move in the opposite direction when the threaded structure is stressed, and an internal limiting ring 315 placed inside the transparent liquid suction tube 21 and capable of highly limiting the upward movement of the built-in piston body 27. When the threaded sleeve 31 is rotated directionally, since the spiral direction of the first threaded structure 34 is opposite to the spiral direction of the second threaded structure 35, and during the rotation process, due to the existence of the polygonal limiting rod 311, the first external threaded rod 36 and the second external threaded rod 37 will not rotate relative to each other. Therefore, the distance between the first external threaded rod 36 and the second external threaded rod 37 will change, thereby driving the internal limiting ring 315 to move inside the piston moving cavity 23. Before inhaling the liquid, by observing the value corresponding to the bottom end of the internal limiting ring 315, it can be indicated that at this time, the maximum single inhalation volume of the somatic cell liquid entering the pipette 1 clamped is reached. When the top end of the built-in piston body 27 abuts against the bottom of the internal limiting ring 315 during the reset process, the built-in piston body 27 cannot continue to move, indicating that the inhalation volume reaches the preset value at this time. When it is necessary to discharge the liquid, by pressing down the top force-applying plate 29, the somatic cell liquid clamped inside the pipette 1 can be completely discharged. When it is necessary to discharge quantitatively, similarly, the threaded sleeve 31 is rotated in the reverse direction. When the difference between the scale value indicated by the bottom of the internal limiting ring 315 or the top of the built-in piston body 27 and the preset value reaches the single quantitative discharge, the rotation of the threaded sleeve 31 can be stopped, thereby achieving the precise control effect of the liquid volume.

[0022] For the specific structure of the threaded metering mechanism 3, please refer to Figure 5 and Figure 6, further comprising a threaded sleeve 31, one end of the threaded sleeve 31 is provided with a first internal thread cavity 32 with a concave structure, the other end of the threaded sleeve 31 is provided with a second internal thread cavity 33 with a concave structure, the rod body of the first external threaded rod 36 is installed inside the first internal thread cavity 32 through a first thread structure 34, the rod body of the second external threaded rod 37 is installed inside the second internal thread cavity 33 through a second thread structure 35, the opposite ends of the first external threaded rod 36 and the second external threaded rod 37 are provided with a polygonal limiting cavity 310 with a concave structure, the center of the threaded sleeve 31 is fixedly installed with a polygonal limiting rod 311 inserted into the polygonal limiting cavity 310, one end of the first external threaded rod 36 is provided with a first connecting plate 38 integrally formed therewith, one end of the second external threaded rod 37 is provided with a second connecting plate 39 integrally formed therewith, the bottom end of the first connecting plate 38 is installed with a fixing collar 312 fixed to the bottom area of the transparent liquid suction tube 21, the top end of the second connecting plate 39 is fixedly installed with a top connecting plate 313, the bottom surface of the top connecting plate 313 is fixedly installed with a longitudinal limiting rod 314 passing through the third rod body perforation 211 and the second rod body perforation 26, the bottom end of the longitudinal limiting rod 314 located inside the piston moving cavity 23 is fixedly installed with a built-in limiting ring 315 capable of axially moving along the piston moving cavity 23, the center of the built-in limiting ring 315 is provided with a rod body moving perforation 316 for the longitudinal pull rod 28 to move, the structural shape of the cross-section of the polygonal limiting cavity 310 is the same as that of the cross-section of the polygonal limiting rod 311, both are polygonal structures, and the structural size of the cross-section of the polygonal limiting cavity 310 matches the structural size of the cross-section of the polygonal limiting rod 311, the first thread structure 34 includes an internal thread structure provided inside the first internal thread cavity 32 and an external thread structure provided on the rod body of the first external threaded rod 36, the second thread structure 35 includes an internal thread structure provided inside the second internal thread cavity 33 and an external thread structure provided on the rod body of the second external threaded rod 37, and the spiral direction of the first thread structure 34 is opposite to the spiral direction of the second thread structure 35.

[0023] In use, the top plug of the pipette 1 can be inserted into a sterile filter, and then the plug of the sterile filter can be inserted into the pipe clip inlet 24, which can effectively prevent contamination and ensure the sterility of the cell culture and processing process. Rotate the threaded sleeve 31 directionally, thereby driving the built-in limit ring 315 to move inside the piston moving cavity 23. Before inhaling the liquid, by observing the value corresponding to the bottom end of the built-in limit ring 315, it can indicate the maximum single inhalation volume of the somatic cell liquid entering and being clamped inside the pipette 1 at this time. Press down the top force application plate 29 until the built-in piston body 27 moves to the lowest point of the piston moving cavity 23, and then insert the bottom suction port of the pipette 1 below the liquid level of the somatic cell liquid. Release the top force application plate 29. Under the elastic action of the spiral spring 210, the top force application plate 29 will drive the built-in piston body 27 to reset upward. At this time, the somatic cell liquid will be inhaled into the pipette 1. When the top end of the built-in piston body 27 abuts against the bottom of the built-in limit ring 315 during the reset process, the built-in piston body 27 cannot move further, indicating that the inhalation volume reaches the preset value at this time. When it is necessary to discharge the liquid, by pressing down the top force application plate 29, the somatic cell liquid clamped inside the pipette 1 can be completely discharged. When it is necessary to discharge quantitatively, rotate the threaded sleeve 31 in the reverse direction in the same way. When the difference between the scale value indicated by the bottom of the built-in limit ring 315 or the top of the built-in piston body 27 and the preset value reaches the single quantitative discharge, the rotation of the threaded sleeve 31 can be stopped.

[0024] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A somatic cell immunotherapy vacuum pipette, comprising a pipette (1) capable of inhaling liquid and storing the liquid inside thereof, characterized in that: It also includes, a piston-type pipetting mechanism (2), which is internally provided with a transparent pipette tube (21) with a hollow and transparent structure, an internal piston body (27) placed inside the transparent pipette tube (21) and capable of moving axially along the transparent pipette tube (21) to change the air pressure inside the pipette tube (1), and a capacity scale value (22) provided on the outer circumferential surface of the transparent pipette tube (21) and capable of reflecting the volume of liquid entering the inside of the pipette tube (1); and a threaded metering mechanism (3), which is internally provided with a first external threaded rod (36) and a second external threaded rod (37) that move in the reverse direction when the threaded structure is stressed, and an internal limiting ring (315) placed inside the transparent pipette tube (21) and capable of imposing a height limit on the upward movement of the internal piston body (27).

2. The somatic cell immunotherapy vacuum pipette according to claim 1, wherein: The piston-type pipetting mechanism (2) further includes a helical spring (210). The inside of the transparent pipette tube (21) is provided with a piston moving cavity (23) with an open bottom end. The transparent pipette tube (21) is provided with a pipe card entrance (24) at the bottom opening end of the piston moving cavity (23) that can be inserted into the top plug of the pipette tube (1). The outer circumferential surface of the transparent pipette tube (21) is provided with longitudinally distributed capacity scale values (22). A first rod body perforation (25) is provided at the center of the top end of the transparent pipette tube (21). A second rod body perforation (26) is provided in the structure near the edge of the top of the transparent pipette tube (21). An internal piston body (27) capable of moving axially along the piston moving cavity (23) is placed inside the transparent pipette tube (21) in the piston moving cavity (23). A longitudinal pull rod (28) passing through the first rod body perforation (25) is fixedly installed at the top end of the internal piston body (27). A top force application plate (29) is fixedly installed at the top end of the longitudinal pull rod (28). A third rod body perforation (211) longitudinally corresponding to the second rod body perforation (26) is provided in the plate body of the top force application plate (29). A helical spring (210) in a compressed state is sleeved on the outer periphery of the rod body of the longitudinal pull rod (28) between the transparent pipette tube (21) and the top force application plate (29).

3. The somatic cell immunotherapy vacuum pipette according to claim 2, characterized in that: The transparent pipette tube (21) is made of transparent PVC material.

4. The somatic cell immunotherapy vacuum pipette according to claim 3, wherein: The sizes of the pipe card entrance (24) and the top plug of the pipette tube (1) to be inserted are matched with the sizes of the two end joints of the corresponding sterile filter.

5. The somatic cell immunotherapy vacuum pipette according to claim 4, wherein: The value shown by the capacity scale value (22) corresponding to the top end face of the internal piston body (27) is the amount of liquid entering the inside of the pipette tube (1).

6. The somatic cell immunotherapy vacuum pipette according to claim 5, characterized in that: The screw-type metering mechanism (3) further includes a screw sleeve (31). One end of the screw sleeve (31) is provided with a first internal thread cavity (32) with a concave structure, and the other end of the screw sleeve (31) is provided with a second internal thread cavity (33) with a concave structure. The rod body of the first external threaded rod (36) is installed inside the first internal thread cavity (32) through a first thread structure (34), and the rod body of the second external threaded rod (37) is installed inside the second internal thread cavity (33) through a second thread structure (35). The opposite ends of the first external threaded rod (36) and the second external threaded rod (37) are provided with a polygonal limiting cavity (310) with a concave structure. A polygonal limiting rod (311) inserted into the polygonal limiting cavity (310) is fixedly installed at the center of the screw sleeve (31). One end of the first external threaded rod (36) is provided with a first connecting plate (38) integrally formed therewith, and one end of the second external threaded rod (37) is provided with a second connecting plate (39) integrally formed therewith. A fixed collar (312) fixed to the bottom area of the transparent liquid suction pipe (21) is installed at the bottom end of the first connecting plate (38). A top connecting plate (313) is fixedly installed at the top end of the second connecting plate (39). A longitudinal limiting rod (314) penetrating through the third rod body perforation (211) and the second rod body perforation (26) is fixedly installed on the bottom surface of the top connecting plate (313). An internal limiting ring (315) capable of axially moving along the piston moving cavity (23) is fixedly installed at the bottom end of the longitudinal limiting rod (314) located inside the piston moving cavity (23). A rod body moving perforation (316) for the longitudinal pull rod (28) to move is provided at the center of the internal limiting ring (315).

7. A somatic cell immunotherapy vacuum pipette according to claim 6, characterized in that: The cross-sectional structure shape of the polygonal limiting cavity (310) is the same as that of the cross-section of the polygonal limiting rod (311), both are polygonal structures, and the cross-sectional structure size of the polygonal limiting cavity (310) matches the cross-sectional structure size of the polygonal limiting rod (311).

8. A somatic cell immunotherapy vacuum pipette according to claim 7, characterized in that: The first thread structure (34) includes an internal thread structure provided inside the first internal thread cavity (32) and an external thread structure provided on the rod body of the first external threaded rod (36). The second thread structure (35) includes an internal thread structure provided inside the second internal thread cavity (33) and an external thread structure provided on the rod body of the second external threaded rod (37), and the spiral direction of the first thread structure (34) is opposite to the spiral direction of the second thread structure (35).

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