T-cell autophagy centrifugal device for preventing cell injury and centrifugal method of t-cell autophagy centrifugal device

Abstract

The invention relates to the technical field of centrifugal devices, and provides a t cell autophagy centrifugal device capable of preventing cell damage and a centrifugal method thereof.The t cell autophagy centrifugal device comprises a supporting assembly, the supporting assembly comprises a base, guide sleeves and guide rods, the guide rods are fixed to the corners of the top end of the base, and the outer sides of the bottoms of the guide rods are slidably connected with the guide sleeves. By arranging the transmission structure, the driving shaft rotates to drive the driving fluted disc to rotate, the driving fluted disc rotates to drive the first transmission fluted disc to rotate, the first transmission fluted disc drives the second transmission fluted disc to rotate through the transmission shaft, the second transmission fluted disc rotates to drive the linkage fluted disc to rotate, and a power source for ascending is provided for the lifting structure; according to the t cell autophagy centrifugal device capable of preventing the cell injury, the waste of resources is reduced, the function that the device lifts while driving the centrifugal assembly to rotate is achieved, and the working efficiency and the centrifugal efficiency of the t cell autophagy centrifugal device capable of preventing the cell injury during use are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of centrifugal devices, and particularly relates to a T-cell autophagy centrifugal device for preventing cell damage and a centrifugation method thereof. Background Art

[0002] As an important part of the immune system, T cells play an important role in resisting pathogens and maintaining the body's homeostasis. However, T cells may also be damaged by various factors, leading to a decline or loss of their functions. Therefore, it is necessary to design a T-cell autophagy centrifugal device for preventing cell damage;

[0003] To this end, the patent with the publication number CN220177192U discloses a cell centrifuge. An installation box is provided inside a housing. A sleeve driven to rotate by a first motor is installed on the top wall of the installation box. A hollow mounting seat is fixedly installed at the upper end of the sleeve. A plurality of placement cylinders are evenly spaced around the axis of the sleeve on the outside of the mounting seat. Centrifuge tubes are provided inside the placement cylinders. A connecting handle is fixedly installed on the placement cylinder. The middle part of the connecting handle is rotatably installed on the mounting seat through a horizontally extending small shaft. An activity column arranged coaxially is movably installed inside the sleeve. A connecting seat is rotatably installed at the upper end of the activity column. One end of the connecting handle away from the placement cylinder is hinged to the connecting seat. In this application, a plurality of centrifuge tubes can be respectively placed inside the placement cylinders. The first motor drives the sleeve and the mounting seat to rotate. The mounting seat drives the plurality of placement cylinders and the centrifuge tubes to rotate, so as to simultaneously perform centrifugation operations on the cells in the plurality of centrifuge tubes, and the efficiency of cell centrifugation is higher;

[0004] When the above-mentioned cell centrifuge is in use, a separate power source needs to be equipped for lifting and rotating, which consumes more resources during use and has low environmental friendliness. Therefore, it is necessary to design a T-cell autophagy centrifugal device for preventing cell damage. Summary of the Invention

[0005] The purpose of the present invention is to provide a T-cell autophagy centrifugal device for preventing cell damage and a centrifugation method thereof, so as to solve the defect that the existing T-cell autophagy centrifugal device needs to be equipped with a separate power source for lifting and rotating during use, consumes more resources during use, and has low environmental friendliness.

[0006] In order to solve the above technical problems, the present invention provides the following technical solution: A T-cell autophagy centrifugal device for preventing cell damage, comprising a support assembly;

[0007] The support assembly includes a base, a guide sleeve and a guide rod. Guide rods are fixed at the corner positions of the top end of the base. Guide sleeves are slidably connected to the outer sides of the bottoms of the guide rods;

[0008] A lifting structure is fixed to the top end of the base. Both sides of the top end of the lifting structure are fixed with transmission structures. The transmission structure includes a second transmission gear disc, a transmission shaft, a second mounting plate, a first mounting plate, a first transmission gear disc and a driving gear disc. The driving gear disc is fixed to the outer wall of the driving shaft near one end of the driving motor. First mounting plates are fixed to the bottom ends of the lifting structures on both sides of the driving gear disc. A first transmission gear disc is rotatably connected to one side of each first mounting plate close to the driving gear disc. A second mounting plate is arranged on one side of each first mounting plate away from the first transmission gear disc. A second transmission gear disc is rotatably connected to one side of each second mounting plate away from the first mounting plate. A transmission shaft is rotatably connected to the interiors of both the second mounting plate and the first mounting plate;

[0009] Centrifugal components are fixed to the top ends of both the second mounting plate and the first mounting plate. The centrifugal component includes a centrifugal box, an inner cavity, a cover body and a fixing plate. The fixing plate is fixed to the top ends of the second mounting plate and the first mounting plate. A centrifugal box is fixed to the top end of the fixing plate. An inner cavity is formed inside the centrifugal box. A cover body is threadedly connected to the outer wall of the top of the centrifugal box;

[0010] A pressing structure is arranged at the top of the inner cavity. A rotating component is fixed to the inner wall of the bottom of the centrifugal box in the inner cavity. The rotating component includes a driving motor, a fixing seat, a turntable and a driving shaft. The driving motor is installed at the bottom end of the fixing plate. The fixing seat is fixed to the inner wall of the bottom of the centrifugal box in the inner cavity. A turntable is rotatably connected to the top end of the fixing seat. A driving shaft is fixed to the bottom end of the turntable;

[0011] A limiting structure is fixed to the top end of the turntable. Test tubes are evenly arranged inside the limiting structure.

[0012] Further, the lifting structure includes a support plate, a threaded sleeve, a linkage gear disc and a lead screw. The support plate is arranged above the base. Threaded sleeves are rotatably connected to both sides inside the support plate. Linkage gear discs are fixed to the top ends of the threaded sleeves. A lead screw passes through both the threaded sleeve and the linkage gear disc.

[0013] Further, the support plate is slidably connected to a guide rod. The threaded sleeves are symmetrically distributed on both sides of the support plate. The threaded sleeve is threadedly connected to the lead screw.

[0014] Further, the pressing structure includes a turning handle, a turning rod and a pressing plate. The pressing plate is arranged at the top of the inner cavity. A turning rod is rotatably connected to the top of the pressing plate. The top end of the turning rod extends to the outside of the cover body and is fixed with a turning handle.

[0015] Furthermore, the limiting structure includes a limiting seat, a limiting groove, a sponge, a card slot, a card block and a limiting plate. The limiting seat is fixed to the top of the turntable, the limiting grooves are evenly arranged on the top of the limiting seat, the inner side of the limiting grooves is provided with sponges, the top of the sponges extends to the outside of the limiting seat and is fixed with the limiting plate, the card slots are evenly arranged on the top of the limiting seat outside the limiting grooves, and the inside of the card slots is provided with card blocks.

[0016] Furthermore, the inner wall of the sponge and the outer wall of the test tube are in conflict with each other, the clamping block and the limiting seat form a clamping structure through a clamping groove, and the top end of the clamping block extends to the outside of the limiting seat and is fixedly connected to the bottom end of the limiting plate.

[0017] Furthermore, the bottom end of the driving shaft extends to the outside of the fixing plate and is fixedly connected to the output end of the driving motor.

[0018] Furthermore, one end of the transmission shaft extends to the outside of the second mounting plate and is fixedly connected to one side of the second transmission gear disc, and the other end of the transmission shaft extends to the outside of the first mounting plate and is fixedly connected to one side of the first transmission gear disc.

[0019] Furthermore, one side of the bottom of the second transmission gear disc is meshed and connected with one side of the linkage gear disc, and one side of the top of the first transmission gear disc is meshed and connected with one side of the driving gear disc.

[0020] A centrifugation method for a T cell autophagy centrifugal device to prevent cell damage, comprising the following steps:

[0021] S1. Place the test tube inside the sponge. The sponge can automatically adapt to the size of the test tube according to the size of the test tube, limit the bottom of the test tube, then screw the cover body and the centrifuge box together, rotate the rotating rod, the rotating rod and the cover body are screwed together, and the rotating rod will drive the pressing plate to descend when rotating, so that the bottom end of the pressing plate and the top end of the test tube contact each other, which limits the test tube;

[0022] S2. Start the drive motor. The rotation of the drive motor will drive the turntable to rotate. The rotation of the turntable can drive the test tube to rotate quickly through the limit seat to centrifuge the cells inside the test tube;

[0023] S3. When the driving shaft rotates, it will drive the driving gear disc to rotate. The rotation of the driving gear disc will drive the second transmission gear disc to rotate. The rotation of the second transmission gear disc will drive the linkage gear disc to rotate. When the linkage gear disc rotates, it will drive the threaded sleeve to rotate. When the threaded sleeve rotates, it will move up and down on the outside of the screw rod. When the threaded sleeve moves up and down, it will drive the support plate to move up and down. The movement of the support plate will drive the centrifugal assembly to move upward through the second mounting plate and the first mounting plate.

[0024] The invention provides a T cell autophagy centrifugal device for preventing cell damage, which has the advantages of:

[0025] By setting a transmission structure, when the drive shaft rotates, it drives the drive gear disk to rotate. The rotation of the drive gear disk drives the first transmission gear disk to rotate. The first transmission gear disk drives the second transmission gear disk to rotate through a transmission shaft. The rotation of the second transmission gear disk can drive the linkage gear disk to rotate, providing a power source for the lifting structure, reducing waste of resources, realizing the function that the device can drive the centrifugal component to rise while rotating, and improving the working efficiency and centrifugal efficiency of the T-cell autophagy centrifugal device for preventing cell damage during use;

[0026] By setting a pressing structure, rotate the rotating rod. The rotating rod is threadedly connected to the cover body. When the rotating rod rotates, it drives the pressing plate to descend, so that the bottom end of the pressing plate contacts the top end of the test tube, realizing the function that the device can limit the test tube, and improving the stability of the T-cell autophagy centrifugal device for preventing cell damage during use;

[0027] By setting a limiting structure, place the test tube inside the sponge. The sponge can automatically adapt to the size of the test tube according to its size, limit the bottom of the test tube. The limiting plate and the limiting seat are snap-connected through a card slot and a card block, which is convenient for replacement, realizing the function that the device is convenient for limiting the test tube and convenient for replacement, and improving the convenience of the T-cell autophagy centrifugal device for preventing cell damage during use;

[0028] By setting a lifting structure, when the linkage gear disk rotates, it drives the threaded sleeve to rotate. When the threaded sleeve rotates, it moves up and down on the outside of the lead screw. When the threaded sleeve moves up and down, it drives the support plate to move up and down. The movement of the support plate drives the centrifugal component to move upward through the second mounting plate and the first mounting plate, realizing the function that the device is convenient for driving the centrifugal component and its internal parts to rise and fall, and improving the convenience of the T-cell autophagy centrifugal device for preventing cell damage during use. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a three-dimensional structure schematic diagram of the whole of the present invention;

[0030] Figure 2 It is a front view sectional structure schematic diagram of the present invention;

[0031] Figure 3 It is for the present invention Figure 2 The enlarged structure schematic diagram at A in;

[0032] Figure 4 It is a three-dimensional structure schematic diagram of the first perspective of the front view sectional view of the present invention;

[0033] Figure 5 It is a three-dimensional structure schematic diagram of the second perspective of the front view sectional view of the present invention;

[0034] Figure 6 is a schematic side view structure diagram of the present invention;

[0035] Figure 7 is a three-dimensional structure diagram of the first perspective of the side view section of the present invention;

[0036] Figure 8 is a three-dimensional structure diagram of the second perspective of the side view section of the present invention;

[0037] Figure 9 is a three-dimensional structure diagram of the top view section of the present invention;

[0038] Figure 10 is a three-dimensional structure diagram of the top view section of the present invention.

[0039] Explanation of reference numerals in the figure: 1. Support assembly; 11. Base; 12. Guide sleeve; 13. Guide rod; 2. Lifting structure; 21. Support plate; 22. Threaded sleeve; 23. Linkage gear disc; 24. Lead screw; 3. Centrifugal assembly; 31. Centrifugal box; 32. Inner cavity; 33. Cover body; 34. Fixed plate; 4. Pressing structure; 41. Rotating handle; 42. Rotating rod; 43. Pressing plate; 5. Test tube; 6. Limiting structure; 61. Limiting seat; 62. Limiting groove; 63. Sponge; 64. Card slot; 65. Block; 66. Limiting plate; 7. Rotating assembly; 71. Driving motor; 72. Fixed seat; 73. Turntable; 74. Driving shaft; 8. Transmission structure; 81. Second transmission gear disc; 82. Transmission shaft; 83. Second mounting plate; 84. First mounting plate; 85. First transmission gear disc; 86. Driving gear disc. Detailed implementation manners

[0040] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0041] Please refer to Figures 1 - 10 , a T cell autophagy centrifugation device for preventing cell damage provided by the present invention includes a support assembly 1.

[0042] Refer to Figure 1 , Figure 2 and Figures 4 - 10, the support assembly 1 includes a base 11, a guide sleeve 12 and a guide rod 13. Guide rods 13 are fixed at the corner positions of the top end of the base 11. Guide sleeves 12 are slidably connected to the outer sides of the bottoms of the guide rods 13. An elevating structure 2 is fixed to the top end of the base 11. The elevating structure 2 includes a support plate 21, a threaded sleeve 22, a linkage gear disk 23 and a lead screw 24. The support plate 21 is arranged above the base 11. Threaded sleeves 22 are rotatably connected to both sides inside the support plate 21. Linkage gear disks 23 are fixed to the top ends of the threaded sleeves 22. Lead screws 24 penetrate through the threaded sleeves 22 and the linkage gear disks 23. The support plate 21 is slidably connected to the guide rods 13. The threaded sleeves 22 are symmetrically distributed on both sides of the support plate 21. The threaded sleeves 22 are threadedly connected to the lead screws 24.

[0043] When the linkage gear disk 23 rotates, it drives the threaded sleeve 22 to rotate. When the threaded sleeve 22 rotates, it moves up and down on the outer side of the lead screw 24. When the threaded sleeve 22 moves up and down, it drives the support plate 21 to move up and down. The movement of the support plate 21 drives the centrifugal assembly 3 to move upward through the second mounting plate 83 and the first mounting plate 84.

[0044] Refer to Figure 1 , Figure 2 , Figures 4 - 6 and Figures 9 - 10 , drive structures 8 are fixed to both sides of the top end of the elevating structure 2. The drive structure 8 includes a second drive gear disk 81, a drive shaft 82, a second mounting plate 83, a first mounting plate 84, a first drive gear disk 85 and a drive gear disk 86. The drive gear disk 86 is fixed to the outer wall of one end of the drive shaft 74 close to the drive motor 71. First mounting plates 84 are fixed to the bottom ends of both sides of the elevating structure 2 where the drive gear disk 86 is located. First drive gear disks 85 are rotatably connected to the sides of the first mounting plates 84 close to the drive gear disk 86. Second mounting plates 83 are arranged on the sides of the first mounting plates 84 away from the first drive gear disks 85. Second drive gear disks 81 are rotatably connected to the sides of the second mounting plates 83 away from the first mounting plates 84. Drive shafts 82 are rotatably connected to the second mounting plates 83 and the first mounting plates 84. One end of the drive shaft 82 extends outside the second mounting plate 83 and is fixed to one side of the second drive gear disk 81. The other end of the drive shaft 82 extends outside the first mounting plate 84 and is fixed to one side of the first drive gear disk 85. One side of the bottom of the second drive gear disk 81 is meshed with one side of the linkage gear disk 23. One side of the top of the first drive gear disk 85 is meshed with one side of the drive gear disk 86.

[0045] When the drive shaft 74 rotates, it drives the drive gear disk 86 to rotate. The rotation of the drive gear disk 86 drives the first drive gear disk 85 to rotate. The first drive gear disk 85 drives the second drive gear disk 81 to rotate through the drive shaft 82. The rotation of the second drive gear disk 81 can drive the linkage gear disk 23 to rotate, providing a power source for the elevating structure 2 to rise and reducing waste of resources.

[0046] Refer to Figure 1 、 Figure 2 and Figures 4 - 8 Figures 4 - 8 , centrifugal components 3 are fixed to the tops of both the second mounting plate 83 and the first mounting plate 84. The centrifugal component 3 includes a centrifugal box 31, an inner cavity 32, a cover body 33, and a fixing plate 34. The fixing plate 34 is fixed to the tops of the second mounting plate 83 and the first mounting plate 84. The centrifugal box 31 is fixed to the top of the fixing plate 34. An inner cavity 32 is provided inside the centrifugal box 31. A cover body 33 is threadedly connected to the outer wall at the top of the centrifugal box 31. A pressing structure 4 is provided at the top of the inner cavity 32. The pressing structure 4 includes a turning handle 41, a turning rod 42, and a pressing plate 43. The pressing plate 43 is disposed at the top of the inner cavity 32. The turning rod 42 is rotatably connected to the top of the pressing plate 43. The top of the turning rod 42 extends to the outside of the cover body 33 and is fixed with the turning handle 41.

[0047] Rotate the turning rod 42. The turning rod 42 is threadedly connected to the cover body 33. When the turning rod 42 rotates, it will drive the pressing plate 43 to descend, so that the bottom end of the pressing plate 43 contacts the top end of the test tube 5.

[0048] Refer to Figures 2 - 10 Figures 2 - 10 , a rotating component 7 is fixed to the inner wall of the centrifugal box 31 at the bottom of the inner cavity 32. The rotating component 7 includes a driving motor 71, a fixing seat 72, a turntable 73, and a driving shaft 74. The driving motor 71 is installed at the bottom end of the fixing plate 34. The fixing seat 72 is fixed to the inner wall of the centrifugal box 31 at the bottom of the inner cavity 32. The top end of the fixing seat 72 is rotatably connected to the turntable 73. The bottom end of the turntable 73 is fixed with the driving shaft 74. The bottom end of the driving shaft 74 extends to the outside of the fixing plate 34 and is fixed to the output end of the driving motor 71. A limiting structure 6 is fixed to the top end of the turntable 73. Test tubes 5 are evenly arranged inside the limiting structure 6. The limiting structure 6 includes a limiting seat 61, limiting grooves 62, sponges 63, card slots 64, clamping blocks 65, and limiting plates 66. The limiting seat 61 is fixed to the top end of the turntable 73. Limiting grooves 62 are evenly opened at the top of the limiting seat 61. Sponges 63 are arranged inside the limiting grooves 62. The top ends of the sponges 63 extend to the outside of the limiting seat 61 and are fixed with the limiting plates 66. Card slots 64 are evenly opened at the top of the limiting seat 61 outside the limiting grooves 62. Clamping blocks 65 are arranged inside the card slots 64. The inner walls of the sponges 63 are in contact with the outer walls of the test tubes 5. The clamping blocks 65 and the limiting seat 61 form a clamping structure through the card slots 64. The top ends of the clamping blocks 65 extend to the outside of the limiting seat 61 and are fixed to the bottom ends of the limiting plates 66.

[0049] Place the test tube 5 inside the sponge 63. The sponge 63 can automatically adapt to the size of the test tube 5 according to its size, limit the bottom of the test tube 5. The limiting plate 66 and the limiting seat 61 are snap-connected through the clamping groove 64 and the clamping block 65, which is convenient for replacement. Start the driving motor 71. When the driving motor 71 rotates, it will drive the turntable 73 to rotate. The rotation of the turntable 73 can drive the test tube 5 to rotate rapidly through the limiting seat 61, and centrifuge the cells inside the test tube 5.

[0050] A centrifugation method for a T-cell autophagy centrifugation device for preventing cell damage includes the following steps:

[0051] S1. Place the test tube 5 inside the sponge 63. The sponge 63 can automatically adapt to the size of the test tube 5 according to its size, limit the bottom of the test tube 5. Then, threadedly connect and fix the cover body 33 and the centrifuge box 31. Rotate the rotating rod 42. The rotating rod 42 is threadedly connected to the cover body 33. When the rotating rod 42 rotates, it will drive the pressing plate 43 to descend, so that the bottom end of the pressing plate 43 contacts the top end of the test tube 5, playing a limiting role on the test tube 5.

[0052] S2. Start the driving motor 71. When the driving motor 71 rotates, it will drive the turntable 73 to rotate. The rotation of the turntable 73 can drive the test tube 5 to rotate rapidly through the limiting seat 61, and centrifuge the cells inside the test tube 5.

[0053] S3. When the drive shaft 74 rotates, it will drive the drive gear disk 86 to rotate. The rotation of the drive gear disk 86 will drive the second transmission gear disk 81 to rotate. The rotation of the second transmission gear disk 81 will drive the linkage gear disk 23 to rotate. When the linkage gear disk 23 rotates, it will drive the threaded sleeve 22 to rotate. When the threaded sleeve 22 rotates, it will move up and down on the outside of the lead screw 24. When the threaded sleeve 22 moves up and down, it will drive the support plate 21 to move up and down. The movement of the support plate 21 will drive the centrifuge assembly 3 to move up through the second mounting plate 83 and the first mounting plate 84.

[0054] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A T-cell autophagy centrifugation device for preventing cell damage, comprising a support assembly (1); Characterized in that: The support assembly (1) includes a base (11), a guide sleeve (12) and a guide rod (13). Guide rods (13) are fixed at the corner positions of the top end of the base (11), and guide sleeves (12) are slidably connected to the outer sides of the bottoms of the guide rods (13); A lifting structure (2) is fixed to the top end of the base (11), and transmission structures (8) are fixed to both sides of the top end of the lifting structure (2). The transmission structure (8) includes a second transmission gear (81), a transmission shaft (82), a second mounting plate (83), a first mounting plate (84), a first transmission gear (85) and a driving gear (86). The driving gear (86) is fixed to the outer wall of one end of the driving shaft (74) close to the driving motor (71). First mounting plates (84) are fixed to the bottom ends of the lifting structures (2) on both sides of the driving gear (86). A first transmission gear (85) is rotatably connected to the side of each first mounting plate (84) close to the driving gear (86). Second mounting plates (83) are provided on the sides of the first mounting plates (84) away from the first transmission gears (85). A second transmission gear (81) is rotatably connected to the side of each second mounting plate (83) away from the first mounting plate (84). Transmission shafts (82) are rotatably connected to the interiors of the second mounting plates (83) and the first mounting plates (84); Centrifugation components (3) are fixed to the top ends of the second mounting plates (83) and the first mounting plates (84). The centrifugation component (3) includes a centrifugation box (31), an inner cavity (32), a cover body (33) and a fixing plate (34). The fixing plate (34) is fixed to the top ends of the second mounting plate (83) and the first mounting plate (84). A centrifugation box (31) is fixed to the top end of the fixing plate (34). An inner cavity (32) is formed inside the centrifugation box (31). A cover body (33) is threadedly connected to the outer wall of the top of the centrifugation box (31); A pressing structure (4) is provided at the top of the inner cavity (32), and a rotating component (7) is fixed to the inner wall of the centrifugation box (31) at the bottom of the inner cavity (32). The rotating component (7) includes a driving motor (71), a fixing seat (72), a turntable (73) and a driving shaft (74). The driving motor (71) is installed at the bottom end of the fixing plate (34). The fixing seat (72) is fixed to the inner wall of the centrifugation box (31) at the bottom of the inner cavity (32). A turntable (73) is rotatably connected to the top end of the fixing seat (72). A driving shaft (74) is fixed to the bottom end of the turntable (73); A limiting structure (6) is fixed to the top end of the turntable (73), and test tubes (5) are evenly arranged inside the limiting structure (6).

2. The autophagy centrifuge device for T cells to prevent cell damage according to claim 1, wherein: The lifting structure (2) includes a support plate (21), a threaded sleeve (22), a linkage gear disc (23) and a lead screw (24). The support plate (21) is arranged above the base (11). Both sides inside the support plate (21) are rotatably connected with threaded sleeves (22). The tops of the threaded sleeves (22) are fixedly provided with linkage gear discs (23). The lead screw (24) passes through the threaded sleeves (22) and the linkage gear discs (23).

3. A T-cell autophagy centrifugation device for preventing cell damage according to claim 2, characterized in that: The support plate (21) is slidably connected with the guide rod (13). The threaded sleeves (22) are symmetrically distributed on both sides of the support plate (21). The threaded sleeves (22) are in threaded connection with the lead screw (24).

4. The t-cell autophagy centrifugation device for preventing cell damage according to claim 1, characterized in that: The pressing structure (4) includes a turning handle (41), a turning rod (42) and a pressing plate (43). The pressing plate (43) is arranged at the top of the inner cavity (32). The top of the pressing plate (43) is rotatably connected with a turning rod (42). The top of the turning rod (42) extends to the outside of the cover body (33) and is fixedly provided with a turning handle (41).

5. A T-cell autophagy centrifugation device for preventing cell damage according to claim 1, characterized in that: The limiting structure (6) includes a limiting seat (61), a limiting groove (62), a sponge (63), a clamping groove (64), a clamping block (65) and a limiting plate (66). The limiting seat (61) is fixed to the top end of the turntable (73). The top of the limiting seat (61) is evenly provided with limiting grooves (62). Sponges (63) are arranged on the inner sides of the limiting grooves (62). The tops of the sponges (63) extend to the outside of the limiting seat (61) and are fixedly provided with limiting plates (66). The clamping grooves (64) are evenly opened on the top of the limiting seat (61) outside the limiting grooves (62). Clamping blocks (65) are arranged inside the clamping grooves (64).

6. The autophagy centrifuge device for T cells preventing cell damage according to claim 5, characterized in that: The inner wall of the sponge (63) abuts against the outer wall of the test tube (5). The clamping block (65) and the limiting seat (61) form a clamping structure through the clamping groove (64). The tops of the clamping blocks (65) extend to the outside of the limiting seat (61) and are fixedly connected with the bottom ends of the limiting plates (66).

7. The centrifugation device for T cell autophagy to prevent cell damage according to claim 1, characterized in that: The bottom end of the drive shaft (74) extends to the outside of the fixed plate (34) and is fixedly connected with the output end of the drive motor (71).

8. The centrifugal device for T-cell autophagy to prevent cell damage according to claim 1, characterized in that: One end of the transmission shaft (82) extends to the outside of the second mounting plate (83) and is fixedly connected with one side of the second transmission gear disc (81). The other end of the transmission shaft (82) extends to the outside of the first mounting plate (84) and is fixedly connected with one side of the first transmission gear disc (85).

9. A T-cell autophagy centrifuge device for preventing cell damage according to claim 1, characterized in that: One side of the bottom of the second transmission gear disc (81) is meshed and connected with one side of the linkage gear disc (23). One side of the top of the first transmission gear disc (85) is meshed and connected with one side of the drive gear disc (86).

10. A centrifugation method for using a T-cell autophagy centrifugation device for preventing cell damage as described in claims 2, 4, and 5, comprising the following steps, characterized in that: S1. Place the test tube (5) inside the sponge (63). The sponge (63) can automatically adapt to the size of the test tube (5) according to its size, limit the bottom of the test tube (5), and then threadedly connect and fix the cover body (33) and the centrifuge box (31). Rotate the rotating rod (42). The rotating rod (42) is threadedly connected to the cover body (33). When the rotating rod (42) rotates, it will drive the pressing plate (43) to descend, so that the bottom end of the pressing plate (43) contacts the top end of the test tube (5), playing a limiting role on the test tube (5). S2. Start the drive motor (71). The rotation of the drive motor (71) will drive the turntable (73) to rotate. The rotation of the turntable (73) can drive the test tube (5) to rotate rapidly through the limit seat (61), centrifuging the cells inside the test tube (5). S3. When the drive shaft (74) rotates, it will drive the drive gear disk (86) to rotate. The rotation of the drive gear disk (86) will drive the second drive gear disk (81) to rotate. The rotation of the second drive gear disk (81) will drive the linkage gear disk (23) to rotate. When the linkage gear disk (23) rotates, it will drive the threaded sleeve (22) to rotate. When the threaded sleeve (22) rotates, it will move up and down on the outside of the lead screw (24). When the threaded sleeve (22) moves up and down, it will drive the support plate (21) to move up and down. The movement of the support plate (21) will drive the centrifuge assembly (3) to move upward through the second mounting plate (83) and the first mounting plate (84).

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