Inoculation device

Abstract

The inoculation device comprises an inoculation frame and a placement frame installed on the inoculation frame, the placement frame is provided with a plurality of insertion holes located in the same vertical plane, the inoculation device further comprises a pipe plug taking-down mechanism, the pipe plug taking-down mechanism comprises a plate body, the plate body is provided with through holes opposite to the insertion holes, and the through holes are communicated with the insertion holes. A plurality of through holes are formed in the plate body, horizontal grooves communicated with the through holes are further formed in the plate body, pressing plates are vertically and slidably connected to the horizontal grooves, pressing blocks extending into the through holes are installed on the pressing plates, the plate further comprises an air cylinder fixed to the plate body, and the movable end of the air cylinder penetrates through the horizontal grooves and then is fixedly connected with the pressing plates. The tube plugs of the multiple inoculation tubes are all taken down at a time, then batch operation of inoculation of the multiple inoculation tubes together is rapidly carried out, frequent instrument switching is not needed in the midway, the contact time of strains in the inoculation tubes and the external environment is shortened, and normal growth of passage bacteria is guaranteed.

Description

Technical Field

[0001] The present invention relates to the field of microorganism inoculation, and particularly to an inoculation device. Background Art

[0002] The bacterial inoculation experiment is an important practical link in microbiology teaching. Through this experiment, students can intuitively master the bacterial inoculation technique, understand the growth characteristics of bacteria, and be familiar with the aseptic operation specifications; and before the students' operation, the experimental technicians need to prepare a large number of bacterial strains. During the operation, the bacterial strains in the mother tube are sucked and inoculated into the inoculation tube. By observing the different morphologies of different bacterial strains or bacterial colonies, the bacterial types can be preliminarily judged. For example, the Escherichia coli colonies are milky white, moist, and have neat edges; the Staphylococcus aureus colonies are golden yellow, round, and convex; after this inoculation, it can be used for aspects such as microorganism separation, identification, and bacterial strain preservation.

[0003] In the prior art, the inoculation device includes an inoculation rack and a placement rack installed on the inoculation rack. Multiple inoculation tubes are inserted into the placement rack. In order to avoid contamination caused by long-term exposure of the inoculation tubes to the air before inoculation, the inoculation tubes usually also have tube plugs. During the operation, the inoculation tube is taken off, the tube plug is taken off, and then the mother tube and the inoculation tube are held by hand, and the bacterial strains in the mother tube are inoculated into the inoculation tube. In order to conduct comparative observations, usually multiple inoculation tubes are required each time, and the experimental technician can only hold 1-2 inoculation tubes for inoculation each time, resulting in the need to repeat the operations of taking off the tube plug and replacing the new inoculation tube for inoculation. This operation is not convenient, and depending on the technician's skills and the state at that time, it is easy to cause contamination and is time-consuming and cumbersome. Summary of the Invention

[0004] The present invention aims to provide an inoculation device to solve the problems in the prior art that the operations of repeatedly replacing the inoculation tubes and taking off the tube plugs are required, resulting in cumbersome and inconvenient operation and time consumption.

[0005] To achieve the above object, the present invention provides the following technical solution: An inoculation device includes an inoculation rack and a placement rack installed on the inoculation rack. The placement rack is provided with a plurality of insertion holes located in the same vertical plane, and further includes a tube plug removing mechanism. The tube plug removing mechanism includes a plate body. The plate body is provided with through holes respectively opposite to the plurality of insertion holes. The inside of the plate body is further provided with a horizontal groove communicated with each through hole. A pressing plate is vertically slidably connected to the horizontal groove. The pressing plate is provided with a pressing block extending into the through hole. It further includes a cylinder fixed to the plate body. The movable end of the cylinder passes through the plurality of horizontal grooves and is respectively fixed to the plurality of pressing plates.

[0006] The working principle and beneficial effects of the present invention: In this application, multiple inoculation tubes are respectively inserted into corresponding jacks and clamped. Multiple parts on the plate body respectively penetrate into the multiple inoculation tubes. The stoppers on the inoculation tubes extend into the through holes and move to directly below the pressing plates. The cylinder drives the multiple pressing plates to move downward synchronously. The pressing blocks extend into the corresponding through holes and press the corresponding stoppers tightly in the through holes. Then, the plate body is pulled outwards, so that the multiple stoppers are separated from the inoculation tubes. Then, the plate body is removed. The movable end of the cylinder drives the multiple pressing plates and pressing blocks to move in the reverse direction. The pressing blocks are separated from the stoppers, and the multiple stoppers are poured out of the through holes.

[0007] This application designs multiple jacks for inserting multiple inoculation tubes, and uses a cylinder in cooperation with multiple pressing plates and pressing blocks to complete the removal of the corresponding stoppers at one time. Compared with the prior art that requires replacing the inoculation tubes multiple times and manually removing the stoppers one by one, the efficiency is significantly improved. In addition, in the prior art, the stoppers are removed one by one before the inoculation work, and the operations of taking the inoculation tube - taking the stopper - inoculating are repeated. The frequent switching of instruments results in a large amount of time consumption, and the exposure time of the inoculation tubes and air after inoculation is prolonged, and bacteria are easily contaminated by air. However, this application can perform inoculation on multiple inoculation tubes and remove all the stoppers at one time, and then quickly perform batch inoculation without frequent instrument switching in the middle, reducing the contact time between the strains in the inoculation tubes and the external environment to ensure the normal growth of the strains and improve the experimental accuracy.

[0008] In some embodiments, a rubber ring for clamping the inoculation tube is fixedly sleeved on the jack. When the inoculation tube is inserted into the jack, the inoculation tube squeezes the rubber ring to deform, and the inoculation tube is fixed by the frictional force between the rubber ring and the inoculation tube.

[0009] In some embodiments, the plate body is provided with a vertical groove communicating with the horizontal groove. The bottom of the pressing plate is provided with a T-shaped sliding groove. The top of the pressing block is horizontally slidably connected to the sliding groove through a T-shaped block. A lead screw passing through the pressing block is rotatably connected to the side wall of the sliding groove. The lead screw is threadedly connected to the pressing block. The lead screw extends out through the vertical groove, and the lead screw and the movable end of the cylinder are in different vertical planes.

[0010] This optimized solution is applicable when inoculating multiple inoculation tubes or inoculation tubes of different lengths simultaneously. Due to special inoculation requirements, for example, when a large number of bacteria need to be propagated, long inoculation tubes are selected, and if quick observation or resource saving is required, short inoculation tubes can be selected. Therefore, 1. When a large number of bacterial strains need to be inoculated, the traditional manual inoculation method can only inoculate 1-2 inoculation tubes, which takes a long time. Moreover, holding the bacterial strain tube and the inoculation tube is highly related to the current state and technical proficiency of the inoculator, and it is easy to have unstable operation and long time consumption. 2. Or when inoculating mixed inoculation tubes of different lengths, multiple inoculation tubes are respectively inserted into the jacks, and one ends of the multiple inoculation tubes are aligned in the jacks, while the stoppers at the other ends of the multiple inoculation tubes cannot be aligned. Therefore, when adopting the basic scheme, it is impossible to ensure that the pressing block presses the stopper for extraction operation. For example, for the through hole into which a longer inoculation tube is inserted, at this time, by rotating the lead screw on this side, the top of the pressing block is horizontally slidably connected through the T-shaped block and the chute, so as to limit the pressing block to only slide horizontally. The lead screw drives the pressing block to move away from the inoculation tube, so that the pressing block moves to directly above the stopper on this side. Then, the pressing plate is driven to press down by the air cylinder, and the pressing plate drives the lead screw to move downward in the vertical groove, and the pressing block presses down on the stopper, forming self-locking of the pressing block through the lead screw, so as to prevent sliding during the pressing down process, so as to ensure that the pressing block accurately presses on the stopper and provide a more concentrated pressing force for extracting the stopper.

[0011] In some embodiments, an injection valve is provided between the plate body on the side of each through hole away from the pressing block and the pressing block. When the stopper is pulled outwards and the inoculation tube has not yet detached from the through hole, the injection valve injects inert gas into the through hole, and the stopper blocks the other side of the through hole, so that the inert gas rushes into the inoculation tube. This part of the inert gas forms a short-term gas seal in the inoculation tube, so as to prevent air from entering the inoculation tube during the inoculation interval and causing pollution.

[0012] In some embodiments, the inoculation rack is fixedly provided with an electric push rod for pushing the placement rack to lift and lower. The electric push rod is used to drive the placement rack to lift and lower, and adjust the angle of the inoculation device according to the sitting height position of the person, which is convenient for the inoculation operation and meets the operation needs of operators of different heights.

[0013] In some embodiments, the number of the through holes is 3-5, and they are evenly spaced in the vertical direction. This optimized scheme can simultaneously extract 3-5 stoppers.

[0014] In some embodiments, the hole walls of the through holes and the pressing blocks are both wrapped with rubber pads. The rubber pads are deformed to increase the pressing force.

[0015] In some embodiments, the pressing block is provided with an arc-shaped groove. The arc-shaped groove fits the inoculation tube to increase the pressing force.

[0016] In some embodiments, the plate body is provided with a handle. The handle is convenient for holding the plate body to pull out the stopper. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of an inoculation device of the present application; Figure 2 For Figure 1 The structural schematic diagram when the inoculation tube is sleeved into the through hole of the plate body; Figure 3 For Figure 2 The internal structural schematic diagram of; Figure 4 For Figure 2 The structural schematic diagram when the pressing block presses the pipe plug in; Figure 5 For Figure 4 The structural schematic diagram when the pipe plug is pulled out in; Figure 6 The internal structural schematic diagram of the plate body in Embodiment 2; Figure 7 For Figure 6 The structural schematic diagram after adjusting the pressing block in; Figure 8 For Figure 7 The partial enlarged schematic diagram of; Figure 9 The internal structural schematic diagram of the plate body in Embodiment 3. Specific embodiments

[0018] The following is a further detailed description through specific embodiments: The reference signs in the accompanying drawings of the specification include: inoculation rack 1, placement rack 2, inoculation tube 3, pipe plug 4, rubber ring 5, cylinder 6, plate body 7, handle 8, pressing plate 9, pressing block 10, lead screw 11, vertical groove 12, injection valve 13.

[0019] In the following statements, orientation words such as "left", "right", "up", and "down" are based on the orientation shown in the figure. In practice, if the corresponding structure makes a co-directional change based on the orientation and maintains the relative position unchanged, it does not affect the implementation of the solution.

[0020] Embodiment 1: An inoculation device includes an inoculation rack 1 fixed to the ground or a table, a placement rack 2 fixedly installed on the inoculation rack 1. The placement rack 2 is provided with 3 jacks located in the same vertical plane, and a rubber ring 5 is fixed in each jack.

[0021] It further includes a pipe plug removal mechanism. The pipe plug removal mechanism includes a plate body 7, as shown in Figure 2 and Figure 3 shown. The plate body 7 is provided with through holes respectively opposite to the 3 jacks. A horizontal groove communicating with each through hole is further provided inside the plate body 7. A pressing plate 9 is vertically slidably connected to the horizontal groove. The pressing plate 9 is fixedly installed with a pressing block 10 extending into the through hole. It further includes a cylinder 6 and a handle 8 fixed to the plate body 7. The movable end of the cylinder 6 passes through the 3 horizontal grooves and is respectively fixedly connected to the 3 pressing plates 9.

[0022] As shown in Figure 4 、Figure 5 and Figure 6 As shown in Figure 6 , in the present application, three inoculation tubes 3 are respectively inserted into corresponding jacks, and the inoculation tubes 3 are inserted to the bottom in the jacks, and one ends of the three inoculation tubes 3 are kept aligned. The inoculation tubes 3 squeeze the rubber ring 5 to deform, and the friction force between the rubber ring 5 and the inoculation tubes 3 is used to fix the inoculation tubes 3, and the rubber ring 5 clamps the inoculation tubes 3. Hold the handle 8 and put the three through holes on the plate body 7 into the three inoculation tubes 3 respectively. The stoppers 4 on the inoculation tubes 3 extend into the through holes and move to directly below the pressing plates 9. The three pressing plates 9 are driven by the air cylinder 6 to move downward synchronously, and the pressing blocks 10 extend into the corresponding through holes. The pressing blocks 10 press the corresponding stoppers 4 tightly in the through holes, then pull the plate body 7 outward to make the three stoppers 4 separate from the inoculation tubes 3, and then remove the plate body 7. The movable end of the air cylinder 6 drives the three pressing plates 9 and the pressing blocks 10 to move in the reverse direction, the pressing blocks 10 separate from the stoppers 4, and the three stoppers 4 can be shaken out of the through holes.

[0023] In the present application, the air cylinder 6 cooperates with the three pressing plates 9 and the pressing blocks 10 to complete the removal of the three stoppers 4 at one time. Compared with the prior art that requires manual removal of the stoppers 4 one by one, the efficiency is obviously improved. In addition, in the prior art, the stoppers 4 are removed one by one and then the inoculation work is carried out. The repeated operation of stopper 4 - inoculation, and the frequent switching of instruments (such as burettes) result in a large amount of time consumption, and the exposure time of the inoculation tubes 3 and the air after inoculation is prolonged, and the bacterial species are easily contaminated by air. However, in the present application, the three stoppers 4 are all removed at one time, and then batch inoculation is carried out quickly. There is no need to perform frequent instrument switching in the middle, reducing the contact time between the bacterial liquid in the inoculation tubes 3 and the external environment to ensure the normal growth of the bacterial liquid and improve the experimental accuracy.

[0024] Embodiment 2: The difference from Embodiment 1 is that the plate body 7 is provided with vertical grooves 12 communicating with each horizontal groove. The vertical grooves 12 provide a space for the screw rods 11 to slide up and down. The bottom of the pressing plate 9 is provided with a T-shaped sliding groove, and the top of the pressing block 10 is fixed with a T-shaped block. The horizontal sliding connection is realized through the T-shaped block and the sliding groove. The side wall of the sliding groove is rotatably connected with a screw rod 11 passing through the pressing block 10. The screw rod 11 is threadedly connected with the pressing block 10. The screw rod 11 extends out through the vertical groove 12, and the screw rod 11 and the movable end of the air cylinder 6 are in different vertical planes.

[0025] This optimized solution is applicable when inoculating different lengths of inoculation tubes 3 at the same time. Due to special inoculation requirements, for example, when a large number of bacteria need to be propagated, long inoculation tubes 3 are selected, and if quick observation or resource saving is required, short inoculation tubes 3 can be selected.

[0026] Such as Figure 6 、 Figure 7 and Figure 8As shown, when inoculation tubes 3 of different lengths are mixed for inoculation, the 3 inoculation tubes 3 are respectively inserted into the jacks, and one ends of the 3 inoculation tubes 3 are aligned within the jacks. However, the stoppers 4 at the other ends of the 3 inoculation tubes 3 cannot be aligned. Therefore, when the basic solution is adopted, it is impossible to ensure that the pressing block 10 presses the stopper 4 for extraction. For example, for the through hole into which a longer inoculation tube 3 is inserted, at this time, by rotating the lead screw 11 on this side, the top of the pressing block 10 is horizontally slidably connected through the T-shaped block and the chute, thereby restricting the pressing block 10 to only slide horizontally. The lead screw 11 drives the pressing block 10 to move away from the inoculation tube 3, so that the pressing block 10 moves to directly above the stopper 4 on this side. Then, the cylinder 6 is used to drive the pressing plate 9 to press down. The pressing plate 9 drives the lead screw 11 to move downward in the vertical groove 12, and the pressing block 10 presses down the stopper 4. The lead screw 11 forms self-locking for the pressing block 10, thereby preventing sliding during the downward pressing process to ensure that the pressing block 10 accurately presses on the stopper 4 and provides a more concentrated pressing force for extracting the stopper 4.

[0027] Embodiment 3: The difference from Embodiment 1 is that, as Figure 9 shown, an injection valve 13 is provided between the plate body 7 and the pressing block 10 on the side of each through hole away from the pressing block 10. After the stopper 4 is pulled outwards, when the inoculation tube 3 has not yet disengaged from the through hole, the injection valve 13 injects an inert gas (such as nitrogen) into the through hole, and the stopper 4 blocks the other side of the through hole, causing the inert gas to rush into the inoculation tube 3. This part of the inert gas forms a temporary gas seal within the inoculation tube 3, thereby preventing air from entering the inoculation tube 3 during the inoculation interval and causing contamination.

[0028] The standard parts used in the present invention can all be purchased from the market. The special-shaped parts can be customized according to the descriptions in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts, and equipment all adopt conventional models in the prior art. In addition, the circuit connection adopts the conventional connection method in the prior art, which will not be elaborated here.

Claims

1. An inoculation device, comprising an inoculation rack and a placement rack installed on the inoculation rack, wherein the placement rack is provided with a plurality of jacks located in the same vertical plane, and is characterized in that: It further includes a plug removing mechanism. The plug removing mechanism includes a plate body. The plate body is provided with through holes respectively opposite to the plurality of jacks. A horizontal groove communicated with each through hole is further arranged inside the plate body. A pressing plate is vertically slidably connected to the horizontal groove. A pressing block extending into the through hole is installed on the pressing plate. It further includes a cylinder fixed to the plate body. The movable end of the cylinder passes through the plurality of horizontal grooves and is respectively fixedly connected to the plurality of pressing plates.

2. The inoculation device according to claim 1, characterized in that: A rubber ring for clamping the inoculation tube is fixedly sleeved on the jack.

3. The inoculation device according to claim 2, characterized in that: The plate body is provided with a vertical groove communicated with the horizontal groove. The bottom of the pressing plate is provided with a T-shaped sliding groove. The top of the pressing block is horizontally slidably connected through a T-shaped block and the sliding groove. A lead screw passing through the pressing block is rotatably connected to the side wall of the sliding groove. The lead screw is threadedly connected to the pressing block. The lead screw extends out through the vertical groove. The lead screw and the movable end of the cylinder are in different vertical planes.

4. The inoculation device according to any one of claims 1 to 3, characterized in that: An injection valve is arranged between one side of the plate body away from the pressing block and the pressing block for each through hole.

5. The inoculation device according to claim 4, characterized in that: The inoculation rack is fixedly provided with an electric push rod for pushing the placement rack to lift.

6. The inoculation device according to claim 5, characterized in that: The number of the through holes is 3 to 5, and they are evenly spaced in the vertical direction.

7. The inoculation device according to claim 6, wherein: The hole walls of the through holes and the pressing blocks are both wrapped with rubber pads.

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