Intelligent enrichment system suitable for PHA synthetic flora
Through the intelligent enrichment system, the synergy of multiple modules is used to solve the problem of unstable bacterial enrichment efficiency in traditional methods, and efficient and stable PHA synthetic bacterial enrichment is achieved, which improves PHA production efficiency.
Patent Information
- Application Number
- CN202510444904.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-10
- Publication Date
- 2025-07-11
AI Technical Summary
The traditional PHA synthesis bacteria enrichment method has complicated steps, long process cycles and many influencing factors, resulting in unstable enrichment efficiency.
An intelligent enrichment system is adopted, including PLC treatment module, dissolved oxygen treatment module, aeration module, feed regulation module, defoaming module, mud scraping module, stirring module, mud discharge module and precipitation drainage module, and efficient and stable bacterial enrichment is achieved through remote monitoring and regulation.
The efficient and stable enrichment of PHA synthetic bacteria was achieved, and the yield of PHA was significantly improved by mixing bacteria.
Smart Images
Figure CN120290299A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the production field of biodegradable plastic polyhydroxyalkanoates (PHA). Specifically, it relates to an intelligent enrichment system for PHA-synthesizing microbial communities. Background Art
[0002] Polyhydroxyalkanoates (PHA) are high molecular polymers synthesized intracellularly by microorganisms. The fully biodegradable properties of PHA meet both the requirements of full biodegradation and the performance requirements of plastic products, and thus have been widely studied. Enriching PHA-synthesizing microbial communities from activated sludge is a decisive step in the three-stage synthesis process of PHA. The goal of this stage is to increase the relative abundance of PHA-synthesizing bacteria in the mixed microbial community and the biomass of the mixed microbial community. However, traditional enrichment methods are complicated in steps, have a long process cycle, and have too many influencing factors, resulting in unstable enrichment efficiency. Therefore, it is necessary to develop a stable and efficient intelligent enrichment system for PHA-synthesizing microbial communities. Summary of the Invention
[0003] The purpose of the present invention is to provide an intelligent enrichment system for PHA-synthesizing microbial communities, which can stably and efficiently enrich PHA-synthesizing microbial communities and improve PHA production efficiency. Based on the characteristics of the PHA-synthesizing microbial community enrichment process, the following main technologies are realized, mainly including:
[0004] 1) A PLC processing module (1) for data processing and regulating various modules during the process of enriching PHA-synthesizing microbial communities in the enrichment reactor;
[0005] 2) A dissolved oxygen processing module (2) for real-time measurement of the dissolved oxygen concentration in the enrichment reactor;
[0006] 3) An aeration module (3) for regulating the aeration power to control the dissolved oxygen concentration in the enrichment reactor;
[0007] 4) A feed regulation module (4) for feeding in the enrichment reactor;
[0008] 5) An antifoaming module (5) for removing the foam generated above the liquid level after adding materials in the enrichment reactor;
[0009] 6) A sludge scraping module (6) for scraping off the microbial flocs above the liquid level in the collection reactor;
[0010] 7) A stirring module (7) for regulating the stirring speed in the enrichment reactor;
[0011] 8) A sludge discharging module (8) for discharging the mixed microorganisms in the enrichment reactor after the enrichment cycle ends;
[0012] 9) The precipitation and drainage module (9) is used to drain the supernatant in the enrichment reactor after the enrichment cycle ends;
[0013] Through the above main technical processes and module designs, when the PHA synthesis bacterial community begins to enrich, the feeding regulation module (4), which is connected to the PLC processing module (1) and is used for feeding in the enrichment reactor, supplements the anaerobic fermentation acid-producing liquid (2000 - 6000 mg COD / L). After feeding, the defoaming module (5) and the sludge scraping module (6) are used to remove the foam generated above the liquid level in the enrichment reactor after feeding and scrape off the microbial flocs above the liquid level in the collection reactor. After the enrichment ends, the stirring module (7) and the aeration module (3) stop operating, and the sludge discharge module (8) and the precipitation and drainage module (9) are connected to the PLC processing module (1) and are used to drain the microbial mixture and excess water in the enrichment reactor after the enrichment cycle ends.
[0014] The present invention is further configured such that: the dissolved oxygen treatment module (2) is connected to the PLC processing module (1) to record the dissolved oxygen concentration in real time during the process of enriching the PHA synthesis bacterial community and output to regulate the aeration module (3); at the same time, the aeration module (3) is connected to the dissolved oxygen treatment module (2) and is used to regulate the aeration power to control the dissolved oxygen concentration in the enrichment reactor, so that the dissolved oxygen is maintained at 5 - 8 mg / L, and the aeration power is increased after the dissolved oxygen is lower than this range; the stirring module (7), which is connected to the PLC processing module (1) and is used to regulate the stirring speed in the enrichment reactor, stops stirring for sludge discharge and drainage after the enrichment cycle ends.
[0015] By using the above technical processes and module designs, the present invention method can remotely collect the corresponding data changes in real time during the enrichment process and remotely regulate the enrichment of the PHA synthesis bacterial community. Through the above operations, the beneficial effect of efficient and stable enrichment of the PHA synthesis bacterial community can be achieved. Description of the Drawings
[0016] The above and / or additional aspects and advantages of the present invention will become obvious and easy to understand from the following description of the embodiments in conjunction with the drawings, where:
[0017] Figure 1 is a schematic diagram of the intelligent enrichment system of the PHA synthesis bacterial community of the present invention.
[0018] Reference numerals: 1, PLC processing module; 2, dissolved oxygen treatment module; 3, aeration module; 4, feeding regulation module; 5, defoaming module; 6, sludge scraping module; 7, stirring module; 8, sludge discharge module; 9, precipitation and drainage module. Detailed Embodiments
[0019] To make the objectives, technical solutions, and advantages of the present invention clearer, the following will clearly and completely describe the technical solutions in the present invention in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention without any creative work belong to the scope of protection of the present invention.
[0020] Figure 1 An intelligent enrichment system for a PHA synthesis microbial community disclosed in the present invention includes a PLC processing module, a dissolved oxygen processing module, an aeration module, a feeding regulation module, an antifoaming module, a sludge scraping module, a stirring module, a sludge discharging module, and a sedimentation and drainage module.
[0021] The PLC processing module (1) is used for data processing and controlling the operation of the PHA synthesis microbial community enrichment system; the dissolved oxygen processing module (2) is connected to the PLC processing module (1) and is used for real-time measurement of the dissolved oxygen concentration in the enrichment reactor. In this embodiment, the aeration module (3) is connected to the dissolved oxygen processing module (2) and is used for regulating the aeration power to control the dissolved oxygen concentration in the enrichment reactor; the feeding regulation module (4), which is connected to the PLC processing module (1), is used for feeding in the enrichment reactor and for regulating the aeration power to control the dissolved oxygen concentration in the enrichment reactor, so that the dissolved oxygen is maintained at 5 - 8 mg / L, and the aeration power is increased when the dissolved oxygen is lower than this range; the feeding regulation module (4), which is connected to the PLC processing module (1), is used for feeding in the enrichment reactor, and anaerobic fermentation acid-producing liquid (2000 - 6000 mg COD / L) is added at the starting stage of each enrichment cycle for enriching the PHA synthesis microbial community; the antifoaming module (5) and the sludge scraping module (6) are both connected to the feeding regulation module (4) and are used for removing the foam generated above the liquid level after feeding in the enrichment reactor and scraping off the microbial flocs above the liquid level in the collection reactor; the stirring module (7) is connected to the PLC processing module (1) and is used for regulating the stirring speed in the enrichment reactor; the sludge discharging module (8) and the sedimentation and drainage module (9) are both connected to the PLC processing module (1) and are used for discharging the mixed microorganisms and supernatant in the enrichment reactor after the enrichment cycle ends.
[0022] In an embodiment of the present invention, the dissolved oxygen processing module (2) is connected to the PLC processing module (1) to record the dissolved oxygen concentration in the process of enriching the PHA synthesis microbial community in real time and output to regulate the aeration module (3).
[0023] In an embodiment of the present invention, the aeration module (3) is connected to the dissolved oxygen processing module (2) and is used for regulating the aeration power to control the dissolved oxygen concentration in the enrichment reactor, so that the dissolved oxygen is maintained at 5 - 8 mg / L, and the aeration power is increased when the dissolved oxygen is lower than this range. After the enrichment cycle ends, aeration is stopped for sludge discharging and drainage.
[0024] In one embodiment of the present invention, a feed regulation module (4) is connected to the PLC processing module (1) and is used to enrich the feed in the reactor. Anaerobic fermentation acidogenic liquid (2000 - 6000 mg COD / L) is added at the starting stage of each enrichment cycle for enriching PHA-synthesizing microbial communities.
[0025] In one embodiment of the present invention, a stirring module (7) is connected to the PLC processing module (1) and is used to regulate the stirring speed in the enrichment reactor. Stirring is stopped for sludge discharge and water drainage after the enrichment cycle ends.
[0026] In one embodiment of the present invention, a sludge discharge module (8) is connected to the PLC processing module (1) and is used to discharge 1 / 10 volume of the mixed microorganisms after the enrichment cycle ends. Sludge discharge is carried out after the aeration module and the stirring module stop.
[0027] In one embodiment of the present invention, a sedimentation and drainage module (9) is connected to the PLC processing module (1) and is used for microbial sedimentation and discharge of excess water after the enrichment cycle ends.
[0028] In one embodiment of the present invention, the sedimentation and drainage module (9) is provided with a liquid level gauge, and after sedimentation and drainage are completed, it feeds back to the PLC processing module (1) to instruct the feed regulation module (4) to replenish the feed.
[0029] The present invention can remotely monitor and intelligently regulate the enrichment reactor to achieve the purpose of efficiently enriching PHA-synthesizing microbial communities, significantly improving the PHA yield of the process for synthesizing PHA by mixed microbial communities.
[0030] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.
[0031] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise specifically defined.
Claims
1. An intelligent enrichment system applicable to PHA synthesis flora, characterized in that, The process characteristics for enriching PHA - synthesizing microbial communities include: A PLC processing module (1) for data processing and regulating various modules during the process of enriching PHA - synthesizing microbial communities in the enrichment reactor; A dissolved - oxygen processing module (2) connected to the PLC processing module (1) and used for real - time measurement of the dissolved - oxygen concentration in the enrichment reactor; An aeration module (3) connected to the dissolved - oxygen processing module (2) and used for regulating the aeration power to control the dissolved - oxygen concentration in the enrichment reactor; A feed - regulation module (4) connected to the PLC processing module (1) and used for feeding in the enrichment reactor; An antifoaming module (5) connected to the feed - regulation module (4) and used for removing the foam generated above the liquid level after feeding in the enrichment reactor; A sludge - scraping module (6) connected to the feed - regulation module (4) and used for scraping off the microbial flocs above the liquid level in the collection reactor; A stirring module (7) connected to the PLC processing module (1) and used for regulating the stirring speed in the enrichment reactor; A sludge - discharging module (8) connected to the PLC processing module (1) and used for discharging the mixed microorganisms in the enrichment reactor after the end of the enrichment cycle; A sedimentation - drainage module (9) connected to the PLC processing module (1) and used for discharging the supernatant in the enrichment reactor after the end of the enrichment cycle.
2. The intelligent enrichment system for PHA synthesis flora according to claim 1, wherein The dissolved - oxygen processing module (2) is connected to the PLC processing module (1) to record the dissolved - oxygen concentration in real - time during the process of enriching PHA - synthesizing microbial communities and output to regulate the aeration module (3).
3. The intelligent enrichment system for PHA-synthesizing flora according to claim 1, wherein, The aeration module (3) is connected to the dissolved - oxygen processing module (2) and used for regulating the aeration power to control the dissolved - oxygen concentration in the enrichment reactor, keeping the dissolved - oxygen at 5 - 8 mg / L. When the dissolved - oxygen is lower than this range, the aeration power is increased, and aeration stops for sludge discharging and water draining after the end of the enrichment cycle.
4. An intelligent enrichment system applicable to PHA synthesis flora according to claim 1, characterized in that, The feed - regulation module (4) is connected to the PLC processing module (1) and used for feeding in the enrichment reactor. Anaerobic fermentation acid - producing liquid with a concentration of 2000 - 6000 mg COD / L is added at the start stage of each enrichment cycle for enriching PHA - synthesizing microbial communities.
5. An intelligent enrichment system for PHA-synthesizing microbial communities according to claim 1, characterized in that The stirring module (7) is connected to the PLC processing module (1) and used for regulating the stirring speed in the enrichment reactor. Stirring stops for sludge discharging and water draining after the end of the enrichment cycle.
6. The intelligent enrichment system applicable to PHA synthesis flora according to claim 1, wherein The sludge - discharging module (8) is connected to the PLC processing module (1) and used for discharging 1 / 10 volume of the mixed microorganisms after the end of the enrichment cycle. Sludge discharging is carried out after the aeration module and the stirring module stop.
7. An intelligent enrichment system for PHA-synthesizing microbial communities according to claim 1, characterized in that, The sedimentation - drainage module (9) is connected to the PLC processing module (1) and used for microbial sedimentation and discharging of excess water after the end of the enrichment cycle.
8. An intelligent enrichment system for PHA-synthesizing microbial communities according to claim 6, characterized in that, The sedimentation - drainage module (9) is equipped with a liquid - level gauge, and after sedimentation and drainage are completed, it feeds back to the PLC processing module (1) to indicate the feed - regulation module (4) to carry out feeding.