Probiotic activity intelligent detection device

Abstract

The invention relates to the related technical field of probiotic activity detection, and discloses an intelligent probiotic activity detection device which comprises an ultra-clean working space, an inner cavity is formed in the inner side of the ultra-clean working space, a partition plate is fixedly connected to the interior of the ultra-clean working space, and a shaking and sampling mechanism is arranged in the inner cavity. A beverage bottle for sampling is arranged on the shake-up sampling mechanism, the beverage bottle comprises an upper bottle body, a concave part, a lower bottle body and a bottle cap, a triangular flask for containing bacterial liquid is placed in an inner cavity, a plurality of test tubes for diluting the bacterial liquid are placed in the inner cavity, a pipettor for transferring the bacterial liquid is placed in the inner cavity, and a plurality of pipetting tips matched with the pipettor are placed in the inner cavity; according to the beverage bottle sampling device, the upper bottle body, the concave part and the lower bottle body of a beverage bottle can be fixed, liquid in the beverage bottle is shaken up, sediment is avoided, and uniform sampling is achieved; in addition, in the coating process, the probiotic activity detection operation can be conveniently carried out in the automatic feeding, discharging and stacking process.

Description

Technical Field

[0001] The present invention belongs to the technical field related to the detection of probiotic activity, and more specifically, particularly relates to an intelligent device for detecting probiotic activity. Background Art

[0002] Probiotics are a class of beneficial active microorganisms to the host that colonize in the human body and change the composition of the flora in a certain part of the host. By regulating the mucosal and systemic immune functions of the host or by regulating the balance of the intestinal flora, they play a role in promoting nutrient absorption and maintaining intestinal health, thereby generating single microorganisms or well-defined mixed microorganisms that are beneficial to health.

[0003] In the prior art, the activity of probiotics is generally detected by the plate counting method. The plate counting method, also known as the dilution plate method, is the most commonly used viable bacteria counting method for measuring the number of living microorganisms in soil. The soil sample is serially diluted and evenly dispersed in the soil suspension, and the microorganisms therein are fully dispersed into single cells. A certain amount of the soil suspension is mixed with the medium before solidification, or evenly spread on the solidified plate medium. The single cells grow and reproduce to form visible colonies with the naked eye, and then the number of microorganisms in the soil is calculated based on the number of colonies formed, the dilution factor, and the sampling amount. This method is simple to operate and is more suitable for counting groups with similar cell sizes and masses, such as bacteria, yeasts, etc.

[0004] The following defects exist in the prior art for the detection of probiotic activity: First, before the existing probiotic beverages leave the factory, their activity needs to be detected. During the sampling process, generally, after manually shaking the probiotic beverage bottle, the bacterial liquid is sampled. However, the manual shaking method cannot thoroughly mix the liquid at the bottom and top of the beverage bottle evenly, which leads to uneven sampling, and further leads to deviations in the detection of probiotic activity. In addition, the middle part of the bottle body of the existing probiotic beverage bottles is generally designed to be concave, thereby improving the strength of the bottle body. However, after the middle part of the bottle body is recessed, it becomes more difficult for the liquid at the bottom and the top to flow, so this method of manually shaking the beverage bottle cannot mix the liquid evenly, and thus uniform sampling cannot be achieved, which is not conducive to the detection of the probiotic activity of the liquid in the beverage bottle.

[0005] Second, after the bacterial liquid is diluted into multiple test tubes, it needs to be transferred to the culture medium and spread. However, during the spreading process, the staff needs to first open the lid of the culture dish, then transfer the diluted bacterial liquid in the test tube to the culture dish through a pipette, then put down the pipette and pick up the spreading rod to spread the diluted bacterial liquid in the culture dish, and finally put down the spreading rod and cover the lid of the culture dish. And to ensure the accuracy of the detection, multiple spreading operations are required, that is, the above steps need to be repeated multiple times, resulting in inconvenience in the detection of probiotic activity.

[0006] Therefore, in view of this, the existing structures and defects are studied and improved to provide an intelligent detection device for probiotic activity, with the aim of achieving a more practical value. Summary of the Invention

[0007] The present invention provides an intelligent detection device for probiotic activity to overcome the above-mentioned defects in the prior art.

[0008] The purpose and efficacy of an intelligent detection device for probiotic activity according to the present invention are achieved by the following specific technical means: An intelligent detection device for probiotic activity includes a super-clean working space. An inner cavity is provided inside the super-clean working space. A partition plate is fixedly connected inside the super-clean working space. A shaking and sampling mechanism is arranged in the inner cavity. A beverage bottle for sampling is arranged on the shaking and sampling mechanism. The beverage bottle includes an upper bottle body, a recessed part, a lower bottle body and a bottle cap. A triangular flask for containing bacterial liquid is placed in the inner cavity. A plurality of test tubes for diluting the bacterial liquid are placed in the inner cavity. A pipette for transferring the bacterial liquid is placed in the inner cavity. A plurality of pipette tips matching the pipette are placed in the inner cavity. A coating mechanism is arranged in the inner cavity. A plurality of culture dishes containing culture medium are placed in the coating mechanism. The culture dish includes a dish body and a dish cover.

[0009] Further technical solution, the shaking and sampling mechanism includes a rotating frame arranged in the inner cavity. A first support seat is fixedly connected to the bottom of the rotating frame. A first worm is rotatably connected inside the first support seat. A first worm gear is arranged outside the first worm. The first worm and the first worm gear are meshed with each other. A left-right threaded lead screw is fixedly connected to the inside of the first worm gear. A second support seat is fixedly connected to the bottom of the rotating frame. The left-right threaded lead screw is rotatably connected to the second support seat. Lead screw nuts are arranged on both sides of the left-right threaded lead screw. A guide plate is arranged outside the lead screw nut. A first clamping plate is fixedly connected to the top of the guide plate. The first clamping plate matches the lower bottle body. The first clamping plate is fixedly connected to a second clamping plate through a first connecting rod. The second clamping plate matches the recessed part. The second clamping plate is fixedly connected to a third clamping plate through a second connecting rod. The third clamping plate matches the upper bottle body.

[0010] Further technical solution, the shaking and sampling mechanism further includes a screw rod fixedly connected to the first worm. A locking gasket and a fixing nut are arranged on the screw rod. A hand wheel is fixedly connected to the end of the screw rod away from the first worm.

[0011] Further technical solution: The shaking and sampling mechanism further includes a support frame disposed below the rotating frame. A first driving motor is fixedly connected to the bottom of the support frame. The output end of the first driving motor is fixedly connected to a second worm. A second worm gear is disposed outside the second worm. A rotating shaft is fixedly connected to the inside of the second worm gear. A chassis is fixedly connected to the bottom of the rotating frame. The rotating shaft is fixedly connected to the chassis. A track is fixedly connected to the top of the support frame. The rotating shaft is rotatably connected to the track. A plurality of balls are disposed inside the track. The chassis is in contact with the balls.

[0012] Further technical solution: The coating mechanism includes a loading rack fixedly connected to the partition plate. A coating table is fixedly connected above the loading rack in the ultra-clean working space. A plurality of first limiting rods are fixedly connected to the edge of the loading rack. A first push plate is disposed inside the plurality of first limiting rods. A plurality of culture dishes are stacked on the first push plate. A first driving cylinder is fixedly connected inside the ultra-clean working space. The output end of the first driving cylinder is fixedly connected to the first push plate.

[0013] Further technical solution: The coating mechanism further includes a third driving cylinder fixedly installed on the outer wall of the ultra-clean working space. The output end of the third driving cylinder is fixedly connected to a C-shaped bracket. Two second driving cylinders are fixedly installed on the C-shaped bracket. The output end of each second driving cylinder is fixedly connected to a clamping arm.

[0014] Further technical solution: The coating mechanism further includes a fourth driving cylinder fixedly installed on the inner wall of the top of the ultra-clean working space. The output end of the fourth driving cylinder is fixedly connected to a gas channel. A vacuum suction cup is communicated with the bottom of the gas channel. A first air extraction pipe is communicated with the outside of the gas channel. A first solenoid valve is disposed on the first air extraction pipe. A first air inlet pipe is communicated with the outside of the gas channel. A second solenoid valve is disposed on the first air inlet pipe.

[0015] Further technical solution: The coating mechanism further includes a V-shaped bracket fixedly connected to the gas channel. One end of the V-shaped bracket is fixedly connected to a fifth driving cylinder. The output end of the fifth driving cylinder is fixedly connected to a connecting plate. A second driving motor is fixedly installed on the top of the connecting plate. The output end of the second driving motor is provided with an MQR rotary joint. A limiting ring is disposed below the MQR rotary joint. A plug rod is disposed inside the limiting ring. A coating rod is inserted at the bottom of the plug rod.

[0016] Further technical solution: The coating mechanism further includes an airbag disposed inside the limiting ring. The outer side of the airbag is communicated with a second air extraction pipe, and a third electromagnetic valve is disposed on the second air extraction pipe. The outer side of the airbag is communicated with a second air inlet pipe, and a fourth electromagnetic valve is disposed on the second air inlet pipe.

[0017] Further technical solution: The coating mechanism further includes a material receiving rack fixedly connected to the ultra-clean working space. A plurality of second limiting rods are disposed at the edge of the material receiving rack, and a second pushing plate is disposed inside the second limiting rods. A sixth driving cylinder is fixedly connected inside the ultra-clean working space, and the output end of the sixth driving cylinder is fixedly connected to the second pushing plate.

[0018] Compared with the prior art, the present invention has the following beneficial effects: In a probiotic activity intelligent detection device of the present invention, through the provided shaking and sampling mechanism, the upper bottle body, the concave part, and the lower bottle body of the beverage bottle can be fixed, and the liquid inside the beverage bottle can be shaken to avoid precipitation. After the liquid inside the beverage bottle is shaken evenly, the staff removes the beverage bottle from the shaking and sampling mechanism, and then pours part of the liquid into the triangular flask to achieve the function of uniform sampling of the bacterial liquid.

[0019] In a probiotic activity intelligent detection device of the present invention, through the provided coating mechanism, the covers of multiple stacked culture dishes can be separated from the tops of the dish bodies in batches, which is convenient for coating the dilution liquid onto the culture medium inside the dish bodies. At the same time, the coating mechanism can evenly apply the dilution liquid onto the culture medium through the coating rod, and after the coating is completed, the coating mechanism can cover the covers back on the tops of the dish bodies and achieve palletizing. This process of automatic feeding, discharging, and palletizing is convenient for the operation of probiotic activity detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is the front view of the overall structure of the present invention; Figure 2 is the schematic structural diagram of the beverage bottle of the present invention; Figure 3 is the layout structural schematic Figure 1 ; Figure 4 is the layout structural schematic Figure 2 ; Figure 5 is the exploded view of the structure of the shaking and sampling mechanism of the present invention; Figure 6 is the bottom view of the structure of the shaking and sampling mechanism of the present invention; Figure 7 is the partial structural schematic Figure 1 ; Figure 8 It is a state diagram when the coating mechanism of the present invention is feeding material; Figure 9 It is a schematic diagram of the partial structure of the coating mechanism of the present invention Figure 2 ; Figure 10 It is a state diagram when the coating mechanism of the present invention is coating; Figure 11 It is a schematic diagram of the partial structure of the coating mechanism of the present invention Figure 3 ; Figure 12 It is a schematic diagram of the partial structure of the coating mechanism of the present invention Figure 4 ; Figure 13 It is a schematic diagram of the partial structure of the coating mechanism of the present invention Figure 5 ; Figure 14 It is a state diagram when the coating mechanism of the present invention is stacking materials; Figure 15 It is a schematic diagram of the partial structure of the coating mechanism of the present invention Figure 6 ; Figure 16 It is a bottom view of the overall structure of the present invention.

[0021] Explanation of reference numerals: 1. Ultra-clean working space; 2. Partition board; 3. Inner cavity; 4. Shaking and sampling mechanism; 401. Rotary rack; 402. First support base; 403. First worm; 404. Screw rod; 405. Locking gasket; 406. Fixed nut; 407. Handwheel; 408. First worm gear; 409. Left-right hand screw rod; 410. Second support base; 411. Screw nut; 412. Guide plate; 413. First clamping plate; 414. First connecting rod; 415. Second clamping plate; 416. Second connecting rod; 417. Third clamping plate; 418. Support frame; 419. First driving motor; 420. Second worm; 421. Second worm gear; 422. Rotating shaft; 423. Chassis; 424. Track; 425. Ball; 5. Erlenmeyer flask; 6. Pipette; 7. Pipette tip; 8. Test tube; 10. Petri dish; 1001. Dish body; 1002. Dish cover; 11. Coating mechanism; 1101. Loading rack; 1102. First limiting rod; 1103. First driving cylinder; 1104. First push plate; 1105. Coating table; 1106. C-shaped bracket; 1107. Second driving cylinder; 1108. Clamping arm; 1109. Third driving cylinder; 1110. Fourth driving cylinder; 1111. Gas channel; 1112. Vacuum chuck; 1113. First exhaust pipe; 1114. First solenoid valve; 1115. First inlet pipe; 1116. Second solenoid valve; 1117. V-shaped bracket; 1118. Fifth driving cylinder; 1119. Connecting plate; 1120. Second driving motor; 1121. MQR rotary joint; 1122. Limiting ring; 1123. Coating rod; 1124. Airbag; 1125. Second exhaust pipe; 1126. Third solenoid valve; 1127. Second inlet pipe; 1128. Fourth solenoid valve; 1129. Receiving rack; 1130. Second limiting rod; 1131. Sixth driving cylinder; 1132. Second push plate; 1133. Inserting rod; 12. Beverage bottle; 1201. Upper bottle body; 1202. Concave part; 1203. Lower bottle body; 1204. Bottle cap. Detailed implementation manners

[0022] The following further describes in detail the implementation manners of the present invention in conjunction with the drawings and embodiments. The following embodiments are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0023] In the description of the present invention, unless otherwise specified, "a plurality of" means two or more; the orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention. In addition, terms such as "first", "second", "third", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0024] In the description of the present invention, it should be noted that, unless otherwise clearly specified and defined, the terms "connected" and "connected to" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] As Figure 1-16 shown, a probiotic activity intelligent detection device includes a super-clean working space 1. An inner cavity 3 is provided inside the super-clean working space 1. A partition plate 2 is fixedly connected inside the super-clean working space 1. A shaking and sampling mechanism 4 is arranged in the inner cavity 3. A beverage bottle 12 for sampling is arranged on the shaking and sampling mechanism 4. The beverage bottle 12 includes an upper bottle body 1201, a recessed part 1202, a lower bottle body 1203 and a bottle cap 1204. A conical flask 5 for containing bacterial liquid is placed in the inner cavity 3. A plurality of test tubes 8 for diluting the bacterial liquid are placed in the inner cavity 3. A pipette 6 for transferring the bacterial liquid is placed in the inner cavity 3. A plurality of pipette tips 7 matching the pipette 6 are placed in the inner cavity 3. A coating mechanism 11 is arranged in the inner cavity 3. A plurality of culture dishes 10 containing a culture medium are placed inside the coating mechanism 11. The culture dish 10 includes a dish body 1001 and a dish cover 1002.

[0026] In this embodiment, first, the shaking and sampling mechanism 4 can fix the upper bottle body 1201, the recessed part 1202, and the lower bottle body 1203 of the beverage bottle 12, and shake the liquid inside the beverage bottle 12 to avoid precipitation. After the liquid inside the beverage bottle 12 is shaken evenly, the staff removes the beverage bottle 12 from the shaking and sampling mechanism 4, and then pours part of the liquid into the Erlenmeyer flask 5 to achieve the function of evenly sampling the bacterial liquid. Subsequently, the pipettor 6 cooperates with the matching pipette tip 7 to dilute the bacterial liquid into multiple test tubes 8. Finally, the coating mechanism 11 can batch-separate the lid 1002 of each of the multiple stacked culture dishes 10 from the top of the dish body 1001, facilitating the coating of the dilution onto the culture medium inside the dish body 1001. At the same time, the coating mechanism 11 can evenly apply the dilution onto the culture medium through the coating rod 1123. Moreover, after the coating is completed, the coating mechanism 11 can cover the lid 1002 back on the top of the dish body 1001 and achieve palletizing.

[0027] Specifically, when implemented, the shaking and sampling mechanism 4 includes a rotating frame 401 disposed in the inner cavity 3. A first support base 402 is fixedly connected to the bottom of the rotating frame 401. A first worm 403 is rotatably connected inside the first support base 402. A first worm gear 408 is disposed outside the first worm 403. The first worm 403 is meshed with the first worm gear 408 in a matching manner. A left-right threaded lead screw 409 is fixedly connected to the inner side of the first worm gear 408. A second support base 410 is fixedly connected to the bottom of the rotating frame 401. The left-right threaded lead screw 409 is rotatably connected to the second support base 410. Screw nuts 411 are disposed on both sides of the left-right threaded lead screw 409. A guide plate 412 is disposed outside the screw nut 411. A first clamping plate 413 is fixedly connected to the top of the guide plate 412. The first clamping plate 413 is matched with the lower bottle body 1203. The first clamping plate 413 is fixedly connected to a second clamping plate 415 through a first connecting rod 414. The second clamping plate 415 is matched with the recessed part 1202. The second clamping plate 415 is fixedly connected to a third clamping plate 417 through a second connecting rod 416. The third clamping plate 417 is matched with the upper bottle body 1201.

[0028] In this embodiment, by rotating the first worm 403, the first worm 403 drives the first worm gear 408 on the outside to rotate, thereby driving the left-right threaded lead screw 409 to rotate on the second support base 410. Subsequently, under the action of the lead screw nut 411, two guide plates 412 are driven to move towards the beverage bottle 12. Since the guide plates 412 are fixedly connected to the first clamping plate 413, the first clamping plate 413 is fixedly connected to the second clamping plate 415 through the first connecting rod 414, and the second clamping plate 415 is fixedly connected to the third clamping plate 417 through the second connecting rod 416, the first clamping plate 413, the second clamping plate 415, and the third clamping plate 417 can be driven to move towards the lower bottle body 1203, the recessed part 1202, and the upper bottle body 1201 of the beverage bottle 12 respectively, and the beverage bottle 12 can be clamped and fixed from both sides, facilitating the fixation of the beverage bottle 12 with a recess in the middle of the bottle body.

[0029] During specific implementation, the shaking and sampling mechanism 4 further includes a screw rod 404 fixedly connected to the first worm 403. A locking gasket 405 and a fixing nut 406 are arranged on the screw rod 404. A handwheel 407 is fixedly connected to the end of the screw rod 404 away from the first worm 403.

[0030] In this embodiment, after the beverage bottle 12 is fixed, the staff can fix the screw rod 404 through the locking gasket 405 and the fixing nut 406. Since the screw rod 404 is fixedly connected to the first worm 403, the first worm 403 can be locked to prevent the first worm 403 from shaking when following the rotation of the rotary frame 401, resulting in insecure fixation of the beverage bottle 12.

[0031] During specific implementation, the shaking and sampling mechanism 4 further includes a support frame 418 arranged below the rotary frame 401. A first driving motor 419 is fixedly connected to the bottom of the support frame 418. A second worm 420 is fixedly connected to the output end of the first driving motor 419. A second worm gear 421 is arranged outside the second worm 420. A rotating shaft 422 is fixedly connected to the inside of the second worm gear 421. A chassis 423 is fixedly connected to the bottom of the rotary frame 401. The rotating shaft 422 is fixedly connected to the chassis 423. A track 424 is fixedly connected to the top of the support frame 418. The rotating shaft 422 is rotatably connected to the track 424. A plurality of balls 425 are arranged inside the track 424. The chassis 423 is in contact with the balls 425.

[0032] In this embodiment, after the first worm gear 403 is locked, the first driving motor 419 on the support frame 418 can be started, so that the second worm gear 420 drives the second worm wheel 421 to rotate, and then drives the rotating shaft 422 fixedly connected to the inside of the second worm wheel 421 to rotate. Since the rotating shaft 422 is fixedly connected to the chassis 423 at the bottom of the rotating frame 401, the rotating frame 401 and the beverage bottle 12 on the rotating frame 401 are driven to rotate, so that the liquid in the beverage bottle 12 can be mixed evenly. In addition, during the rotation of the chassis 423, it will contact the ball 425, thereby improving the stability of the rotating frame 401 during rotation.

[0033] After the beverage bottle 12 is mixed evenly, the liquid inside the beverage bottle 12 is transferred to the Erlenmeyer flask 5, and the sampled bacterial liquid is diluted into multiple test tubes 8 through the pipettor 6 and the pipette tip 7.

[0034] Specifically, the coating mechanism 11 includes a feeding rack 1101 fixedly connected to the partition plate 2. Above the feeding rack 1101, a coating table 1105 is fixedly connected to the super-clean working space 1. A plurality of first limiting rods 1102 are fixedly connected to the edge of the feeding rack 1101. Inside the plurality of first limiting rods 1102, a first pushing plate 1104 is provided. A plurality of culture dishes 10 are stacked and placed on the first pushing plate 1104. A first driving cylinder 1103 is fixedly connected inside the super-clean working space 1, and the output end of the first driving cylinder 1103 is fixedly connected to the first pushing plate 1104.

[0035] In this embodiment, after the bacterial liquid is diluted into multiple test tubes 8, a plurality of stacked culture dishes 10 can be placed on the first pushing plate 1104 from the top, and then the first driving cylinder 1103 is started, so that the first pushing plate 1104 will push the culture dish 10 upward to realize the feeding function. In addition, the plurality of first limiting rods 1102 have a limiting effect on the inner culture dish 10.

[0036] Specifically, the coating mechanism 11 further includes a third driving cylinder 1109 fixedly installed on the outer wall of the super-clean working space 1. The output end of the third driving cylinder 1109 is fixedly connected to a C-shaped bracket 1106. Two second driving cylinders 1107 are fixedly installed on the C-shaped bracket 1106, and the output end of each second driving cylinder 1107 is fixedly connected to a clamping arm 1108.

[0037] In this embodiment, after the culture dish 10 is pushed to above the coating table 1105, the two second driving cylinders 1107 on the C-shaped bracket 1106 will drive the clamping arms 1108 to move towards the dish body 1001 and clamp and fix the dish body 1001 from both sides. Subsequently, the third driving cylinder 1109 will drive the C-shaped bracket 1106 and the culture dish 10 to move to the middle of the coating table 1105.

[0038] During specific implementation, the coating mechanism 11 further includes a fourth driving cylinder 1110 fixedly installed on the inner wall of the top of the ultra-clean working space 1. The output end of the fourth driving cylinder 1110 is fixedly connected to a gas passage 1111. The bottom of the gas passage 1111 is communicated with a vacuum suction cup 1112. The outside of the gas passage 1111 is communicated with a first air extraction pipe 1113. A first electromagnetic valve 1114 is arranged on the first air extraction pipe 1113. The outside of the gas passage 1111 is communicated with a first air inlet pipe 1115. A second electromagnetic valve 1116 is arranged on the first air inlet pipe 1115.

[0039] In this embodiment, when the culture dish 10 moves to the middle of the coating table 1105, the fourth driving cylinder 1110 drives the gas passage 1111 to move downward. The first air extraction pipe 1113 is connected to an external air extraction pump, so that the air extraction pump extracts the air near the vacuum suction cup 1112. At this time, the vacuum suction cup 1112 adsorbs the dish cover 1002. Then the fourth driving cylinder 1110 drives the gas passage 1111 to move upward, thereby driving the dish cover 1002 to move upward and separate from the dish body 1001.

[0040] During specific implementation, the coating mechanism 11 further includes a V-shaped bracket 1117 fixedly connected to the gas passage 1111. One end of the V-shaped bracket 1117 is fixedly connected to a fifth driving cylinder 1118. The output end of the fifth driving cylinder 1118 is fixedly connected to a connecting plate 1119. A second driving motor 1120 is fixedly installed on the top of the connecting plate 1119. The output end of the second driving motor 1120 is provided with an MQR rotary joint 1121. A limiting ring 1122 is arranged below the MQR rotary joint 1121. A plug rod 1133 is arranged inside the limiting ring 1122. The bottom of the plug rod 1133 is inserted with a coating rod 1123.

[0041] In this embodiment, after the dish cover 1002 moves upward and separates from the dish body 1001, the diluted bacterial liquid in the test tube 8 is transferred to the culture medium of the dish body 1001. Then the fifth driving cylinder 1118 on the V-shaped bracket 1117 drives the connecting plate 1119 to move, and thus can drive the coating rod 1123 below the connecting plate 1119 to move above the culture medium. Then the fourth driving cylinder 1110 moves downward and drives the coating rod 1123 to fit the culture medium. Subsequently, the second driving motor 1120 is started, so that the limiting ring 1122 drives the coating rod 1123 below to rotate, so that the diluted bacterial liquid can be evenly distributed on the culture medium, thereby realizing automatic coating of the bacterial liquid and improving the coating effect.

[0042] During specific implementation, the coating mechanism 11 further includes an airbag 1124 disposed inside the limiting ring 1122. The outer side of the airbag 1124 is communicated with a second air extraction pipe 1125, and a third electromagnetic valve 1126 is arranged on the second air extraction pipe 1125. The outer side of the airbag 1124 is communicated with a second air inlet pipe 1127, and a fourth electromagnetic valve 1128 is arranged on the second air inlet pipe 1127.

[0043] In this embodiment, in addition, when it is necessary to fix the coating rod 1123, the coating rod 1123 can be first inserted into the insertion rod 1133, and then the airbag 1124 will be inflated under the action of the second air inlet pipe 1127 and cooperate with the insertion rod 1133 to effectively fix the coating rod 1123, improving the fixing effect on the coating rod 1123.

[0044] After the diluted bacterial liquid is evenly coated on the culture medium under the action of the coating rod 1123, the fourth driving cylinder 1110 moves upward and drives the coating rod 1123 to separate from the culture medium, and then the fifth driving cylinder 1118 drives the coating rod 1123 to reset. After the coating rod 1123 is reset, the fourth driving cylinder 1110 will move downward and drive the dish cover 1002 to dock with the dish body 1001. The second electromagnetic valve 1116 on the first air inlet pipe 1115 is opened, so that the negative pressure inside the vacuum suction cup 1112 disappears, and the dish body 1001 is removed from the vacuum suction cup 1112, and then the fourth driving cylinder 1110 drives the vacuum suction cup 1112 to reset.

[0045] After the dish cover 1002 is docked with the dish body 1001, the third driving cylinder 1109 will drive the culture dish 10 to move above the second push plate 1132.

[0046] During specific implementation, the coating mechanism 11 further includes a material receiving rack 1129 fixedly connected to the ultra-clean working space 1. A plurality of second limiting rods 1130 are arranged at the edge of the material receiving rack 1129, and a second push plate 1132 is arranged inside the second limiting rods 1130. A sixth driving cylinder 1131 is fixedly connected inside the ultra-clean working space 1, and the output end of the sixth driving cylinder 1131 is fixedly connected to the second push plate 1132.

[0047] In this embodiment, after the culture dish 10 moves above the second push plate 1132, the second driving cylinder 1107 on the C-shaped bracket 1106 drives the clamping arm 1108 to reset, so that the clamping arm 1108 will release the culture dish 10, and the culture dish 10 falls on the second push plate 1132. Then the sixth driving cylinder 1131 will drive the second push plate 1132 and the culture dish 10 on the second push plate 1132 to move and stack on the material receiving rack 1129.

[0048] Repeat the above steps to coat the diluents in multiple test tubes 8 onto multiple culture dishes 10. This automated feeding, unloading, and stacking process facilitates the operation of probiotic activity detection. After the cultivation is completed, the number of probiotics can be detected through a magnifying glass or microscope, realizing the function of probiotic activity detection.

[0049] The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described in order to better illustrate the principles of the invention and its practical application, and to enable those of ordinary skill in the art to understand the invention and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. An intelligent detection device for probiotic activity, characterized in that: It includes a super-clean working space, an inner cavity is opened on the inner side of the super-clean working space, a partition board is fixedly connected inside the super-clean working space, a shaking and sampling mechanism is arranged in the inner cavity, a beverage bottle for sampling is arranged on the shaking and sampling mechanism, the beverage bottle includes an upper bottle body, a concave part, a lower bottle body and a bottle cap, an Erlenmeyer flask for containing bacterial liquid is placed in the inner cavity, a plurality of test tubes for diluting the bacterial liquid are placed in the inner cavity, a pipette for transferring the bacterial liquid is placed in the inner cavity, a plurality of pipette tips matching the pipette are placed in the inner cavity, a coating mechanism is arranged in the inner cavity, and a plurality of culture dishes containing culture medium are placed in the coating mechanism. The culture dish includes a dish body and a dish cover.

2. The probiotic activity intelligent detection device according to claim 1, characterized in that: The shaking and sampling mechanism includes a rotating frame arranged in the inner cavity. A first support seat is fixedly connected to the bottom of the rotating frame. A first worm is rotatably connected inside the first support seat. A first worm gear is arranged on the outer side of the first worm. The first worm and the first worm gear are meshed with each other. A left-right threaded lead screw is fixedly connected to the inner side of the first worm gear. A second support seat is fixedly connected to the bottom of the rotating frame. The left-right threaded lead screw is rotatably connected to the second support seat. Lead screw nuts are arranged on both sides of the left-right threaded lead screw. A guide plate is arranged on the outer side of the lead screw nut. A first clamping plate is fixedly connected to the top of the guide plate. The first clamping plate matches the lower bottle body. The first clamping plate is fixedly connected to a second clamping plate through a first connecting rod. The second clamping plate matches the concave part. The second clamping plate is fixedly connected to a third clamping plate through a second connecting rod. The third clamping plate matches the upper bottle body.

3. The intelligent detection device for probiotic activity according to claim 2, characterized in that: The shaking and sampling mechanism further includes a screw rod fixedly connected to the first worm. A locking gasket and a fixing nut are arranged on the screw rod. A hand wheel is fixedly connected to the end of the screw rod away from the first worm.

4. The intelligent detection device for probiotic activity according to claim 2, wherein: The shaking and sampling mechanism further includes a support frame arranged below the rotating frame. A first driving motor is fixedly connected to the bottom of the support frame. The output end of the first driving motor is fixedly connected to a second worm. A second worm gear is arranged on the outer side of the second worm. A rotating shaft is fixedly connected to the inner side of the second worm gear. A chassis is fixedly connected to the bottom of the rotating frame. The rotating shaft is fixedly connected to the chassis. A track is fixedly connected to the top of the support frame. The rotating shaft is rotatably connected to the track. A plurality of balls are arranged inside the track. The chassis is in contact with the balls.

5. The probiotic activity intelligent detection device according to claim 1, wherein: The coating mechanism includes a feeding frame fixedly connected to the partition board. A coating table is fixedly connected above the super-clean working space and located above the feeding frame. A plurality of first limiting rods are fixedly connected to the edge of the feeding frame. A first pushing plate is arranged inside the plurality of first limiting rods. A plurality of culture dishes are stacked and placed on the first pushing plate. A first driving cylinder is fixedly connected inside the super-clean working space. The output end of the first driving cylinder is fixedly connected to the first pushing plate.

6. The probiotic activity intelligent detection device according to claim 1, wherein: The coating mechanism further includes a third driving cylinder fixedly installed on the outer wall of the ultra-clean working space. The output end of the third driving cylinder is fixedly connected to a C-shaped bracket, and two second driving cylinders are fixedly installed on the C-shaped bracket. The output end of each second driving cylinder is fixedly connected to a clamping arm.

7. The probiotic activity intelligent detection device according to claim 1, characterized in that: The coating mechanism further includes a fourth driving cylinder fixedly installed on the inner wall of the top of the ultra-clean working space. The output end of the fourth driving cylinder is fixedly connected to a gas channel. The bottom of the gas channel is communicated with a vacuum suction cup. The outer side of the gas channel is communicated with a first suction pipe, and a first solenoid valve is arranged on the first suction pipe. The outer side of the gas channel is communicated with a first intake pipe, and a second solenoid valve is arranged on the first intake pipe.

8. An intelligent detection device for probiotic activity according to claim 7, characterized in that: The coating mechanism further includes a V-shaped bracket fixedly connected to the gas channel. One end of the V-shaped bracket is fixedly connected to a fifth driving cylinder. The output end of the fifth driving cylinder is fixedly connected to a connecting plate. A second driving motor is fixedly installed on the top of the connecting plate. The output end of the second driving motor is provided with an MQR rotary joint. A limiting ring is arranged below the MQR rotary joint. A plug rod is arranged inside the limiting ring, and a coating rod is inserted at the bottom of the plug rod.

9. The probiotic activity intelligent detection device according to claim 8, wherein: The coating mechanism further includes an airbag arranged inside the limiting ring. The outer side of the airbag is communicated with a second suction pipe, and a third solenoid valve is arranged on the second suction pipe. The outer side of the airbag is communicated with a second intake pipe, and a fourth solenoid valve is arranged on the second intake pipe.

10. The probiotic activity intelligent detection device according to claim 1, wherein: The coating mechanism further includes a material receiving rack fixedly connected to the ultra-clean working space. A plurality of second limiting rods are arranged at the edge of the material receiving rack. A second pushing plate is arranged inside the second limiting rods. A sixth driving cylinder is fixedly connected inside the ultra-clean working space, and the output end of the sixth driving cylinder is fixedly connected to the second pushing plate.

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