High-throughput Raman single cell sorting device and method

Abstract

The invention provides a high-flux Raman single cell sorting device and method.The high-flux Raman single cell sorting device comprises a sorting device body and a micro-fluidic chip, the front side of the sorting device body is movably connected with a connecting door through a hinge, a control panel is fixedly installed on the front side of the sorting device body, a partition plate is fixedly installed in an inner cavity of the sorting device body, and the micro-fluidic chip is fixedly installed in the inner cavity of the sorting device body. An air cylinder is fixedly installed at the bottom of an inner cavity of the sorting device body, a fixing mechanism is arranged in the inner cavity of the sorting device body, the fixing mechanism comprises a box body, the box body is located in the inner cavity of the sorting device body, and by arranging the fixing mechanism, a fixing frame can clamp and fix a micro-fluidic chip inwards; the fixed micro-fluidic chip is convenient for accurately adjusting and optimizing a light path, so that the collection efficiency of Raman signals is improved, and by fixing the micro-fluidic chip, it can be ensured that laser beams are accurately focused on cells, the collection efficiency of Raman scattering is improved, and then the signal strength and quality are enhanced.

Description

Technical Field

[0001] The present invention relates to a high-throughput Raman single-cell sorting device and method, belonging to the technical field of cell sorting devices. Background Art

[0002] Raman spectroscopy is an efficient information recognition technology. By analyzing the inelastic scattering spectrum of a compound by specific incident light, micro-Raman spectroscopy can directly detect the vibrational or rotational energy levels of compound molecules. By analyzing the Raman characteristic spectrum lines, information on the molecular composition and structure of the compound can be obtained.

[0003] When some high-throughput Raman single-cell sorting devices install the microfluidic chip, tape is used to fix its position. However, the tape may leave glue marks on the surface of the microfluidic chip after sticking, and it is inconvenient to clean the glue marks, which may affect the subsequent reuse of the microfluidic chip and is not convenient for the operator to use.

[0004] Therefore, a high-throughput Raman single-cell sorting device and method are proposed. Summary of the Invention

[0005] In view of this, the present invention provides a high-throughput Raman single-cell sorting device and method to solve or alleviate the technical problems existing in the prior art and at least provide a beneficial option.

[0006] The technical solution of the present invention is implemented as follows: A high-throughput Raman single-cell sorting device and method, including a sorting device body and a microfluidic chip. A connecting door is movably connected to the front side of the sorting device body through a hinge. A control panel is fixedly installed on the front side of the sorting device body. A partition is fixedly installed in the inner cavity of the sorting device body. A cylinder is fixedly installed at the bottom of the inner cavity of the sorting device body. A lifting platform is fixedly installed at the top of the cylinder. The microfluidic chip is located in the inner cavity of the sorting device body. A fixing mechanism is arranged in the inner cavity of the sorting device body. The fixing mechanism includes a box body. The box body is located in the inner cavity of the sorting device body. Slide rods are fixedly installed on both the front and rear sides of the inner cavity of the box body. Sliders are slidably connected to the left and right sides of the surfaces of the two slide rods. First rigid springs are fixedly connected to the inner sides of the four sliders. Second rigid springs are fixedly connected to the outer sides of the four sliders. The outer sides of the four second rigid springs are fixedly connected to the left and right sides of the inner cavity of the box body. Connecting frames are fixedly installed on the inner sides of the four sliders. Pull rods are fixedly installed on the outer sides of the two connecting frames. Fixing frames are fixedly installed on the tops of the two connecting frames.

[0007] Further preferably, protective pads are fixedly installed on the inner sides of the two fixing frames. Both protective pads are attached to the left and right sides of the microfluidic chip, and the material of the protective pads is rubber.

[0008] Further preferably, mounting bolts are threadedly connected to both the left and right sides of the box body, and the bottoms of the four mounting bolts are threadedly connected to the inner surface of the lifting platform.

[0009] Further preferably, sliding grooves are formed in both the front and rear sides of the inner cavity of the box body, and the outer sides of the four sliders are slidably connected to the inner cavities of the two sliding grooves.

[0010] Further preferably, a moving groove is formed in the bottom of the inner cavity of the box body, and the bottoms of the two connecting frames are slidably connected to the inner cavity of the moving groove.

[0011] Further preferably, slot holes are formed in both the left and right sides of the inner cavity of the box body, and both the two pull rods penetrate through the inner cavities of the two slot holes and extend to the outside of the box body.

[0012] Further preferably, limiting grooves are formed in both the front and rear sides of the top of the box body, and both the two fixing frames penetrate through the inner cavities of the two limiting grooves and extend to the top of the box body.

[0013] A usage method of a high-throughput Raman single-cell sorting device: includes the following steps: S1: When in use, open the connecting door and pull the pull rod to move outward. The connecting frame and the slider move synchronously to the left and right sides. At this time, the first rigid spring is stretched and deformed, and at the same time, the second rigid spring is also deformed by the extrusion of the slider. The fixing frame moves synchronously to the left and right sides with the connecting frame and prepares for the fixing work of the microfluidic chip.

[0014] S2: Place the microfluidic chip inside the fixing frame. Then, by releasing the pull rod, at this time, the second rigid spring and the first rigid spring lose the influence of force and deform. The first rigid spring and the second rigid spring respectively use their own tensions to make the slider move inward. At this time, the slider drives the connecting frame and the fixing frame to move inward synchronously. The protective pads on the surface of the fixing frame fit against the left and right sides of the microfluidic chip and complete the fixing work on it.

[0015] S3: Through the extension of the cylinder, the lifting platform drives the fixing mechanism and the microfluidic chip to move upward. When the microfluidic chip is in a suitable position, inject the cell suspension into the microfluidic chip. Then, start the sorting work on the cells through the operation of the sorting device body.

[0016] Due to the adoption of the above technical solutions in the embodiments of the present invention, it has the following advantages:

[0017] 1. The present invention sets a fixing mechanism. Through the tension of the first rigid spring and the second rigid spring, the fixing frame can clamp and fix the microfluidic chip inwardly. The fixed microfluidic chip is convenient for precisely adjusting and optimizing the optical path, thereby improving the collection efficiency of Raman signals. By fixing the microfluidic chip, it can ensure that the laser beam is precisely focused on the cells, improve the collection efficiency of Raman scattering, and further enhance the signal intensity and quality.

[0018] 2. The present invention sets a protective pad to avoid direct contact between the fixing frame and the microfluidic chip, thus preventing scratches on the surface and inside of the microfluidic chip. By setting installation bolts, the overall fixing mechanism can be disassembled and assembled, facilitating the repair and maintenance of the components in the fixing mechanism. By setting a sliding groove, the movement of the slider can be limited to prevent deviation during movement, thus affecting the fixing of the microfluidic chip. By setting a moving groove, the moving direction of the connecting frame can be limited to prevent the direction from changing during movement, thus affecting the fixing effect of the microfluidic chip. By setting a slot hole, the movement of the pull rod can be limited to prevent the direction from changing when the pull rod pulls the connecting frame to move, thus affecting the fixing of the microfluidic chip. By setting a limiting groove, the moving direction of the pull rod can be limited to prevent deviation during movement, thus affecting the fixing effect of the microfluidic chip.

[0019] The above summary is only for the purpose of the specification and is not intended to be limiting in any way. In addition to the above-described illustrative aspects, embodiments, and features, further aspects, embodiments, and features of the present invention will become readily apparent by reference to the drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0021] Figure 1 is a schematic front view three-dimensional structure diagram of the present invention;

[0022] Figure 2 is a schematic disassembly structure diagram of the connecting door of the present invention;

[0023] Figure 3 is a schematic structure diagram of the microfluidic chip of the present invention;

[0024] Figure 4 is a schematic disassembly structure diagram of the cylinder of the present invention;

[0025] Figure 5 Schematic structural diagram of the fixing mechanism of the present invention;

[0026] Figure 6 Schematic internal structure diagram of the box body of the present invention.

[0027] Reference numerals: 1, sorting device body; 2, fixing mechanism; 201, box body; 202, slide bar; 203, slider; 204, first rigid spring; 205, second rigid spring; 206, connecting frame; 207, pull rod; 208, fixing frame; 209, protective pad; 210, mounting bolt; 211, chute; 212, moving groove; 213, slot hole; 214, limiting groove; 3, microfluidic chip; 4, control panel; 5, connecting door; 6, partition; 7, cylinder; 8, lifting platform. Detailed implementation manners

[0028] In the following, only some exemplary embodiments are simply described. As those skilled in the art can recognize, the described embodiments can be modified in various different ways without departing from the spirit or scope of the present invention. Therefore, the drawings and the description are regarded as exemplary in nature rather than restrictive.

[0029] The embodiments of the present invention will be described in detail below with reference to the drawings.

[0030] Embodiment 1

[0031] As Figures 1-6 shown, the embodiment of the present invention provides a high-throughput Raman single-cell sorting device and method, including a sorting device body 1 and a microfluidic chip 3. A connecting door 5 is movably connected to the front side of the sorting device body 1 through a hinge. A control panel 4 is fixedly installed on the front side of the sorting device body 1. A partition 6 is fixedly installed in the inner cavity of the sorting device body 1. A cylinder 7 is fixedly installed at the bottom of the inner cavity of the sorting device body 1. A lifting platform 8 is fixedly installed on the top of the cylinder 7. The microfluidic chip 3 is located in the inner cavity of the sorting device body 1. A fixing mechanism 2 is arranged in the inner cavity of the sorting device body 1. The fixing mechanism 2 includes a box body 201. The box body 201 is located in the inner cavity of the sorting device body 1. Slide bars 202 are fixedly installed on both the front and rear sides of the inner cavity of the box body 201. Sliders 203 are slidably connected to the left and right sides of the surfaces of the two slide bars 202. First rigid springs 204 are fixedly connected to the inner sides of the four sliders 203. Second rigid springs 205 are fixedly connected to the outer sides of the four sliders 203. The outer sides of the four second rigid springs 205 are fixedly connected to the left and right sides of the inner cavity of the box body 201. Connecting frames 206 are fixedly installed on the inner sides of the four sliders 203. Pull rods 207 are fixedly installed on the outer sides of the two connecting frames 206. Fixing frames 208 are fixedly installed on the tops of the two connecting frames 206.

[0032] By setting the fixing mechanism 2, through the tension of the first rigid spring 204 and the second rigid spring 205, the fixing frame 208 can clamp and fix the microfluidic chip 3 inwardly. The fixed microfluidic chip 3 is convenient for precisely adjusting and optimizing the optical path, thereby improving the collection efficiency of Raman signals. By fixing the microfluidic chip 3, it can ensure that the laser beam is precisely focused on the cells, improve the collection efficiency of Raman scattering, and further enhance the signal intensity and quality.

[0033] Embodiment 2

[0034] As Figures 3-6 shown, in one embodiment, protective pads 209 are fixedly installed on the inner sides of the two fixing frames 208. The two protective pads 209 are both attached to the left and right sides of the microfluidic chip 3, and the material of the protective pads 209 is rubber. Installation bolts 210 are threadedly connected to the left and right sides of the box body 201. The bottoms of the four installation bolts 210 are threadedly connected to the inner surface of the lifting platform 8. Sliding grooves 211 are opened on the front and rear sides of the inner cavity of the box body 201. The outer sides of the four sliders 203 are slidably connected to the inner cavities of the two sliding grooves 211. A moving groove 212 is opened at the bottom of the inner cavity of the box body 201. The bottoms of the two connecting frames 206 are slidably connected to the inner cavity of the moving groove 212. Slot holes 213 are opened on the left and right sides of the inner cavity of the box body 201. The two pull rods 207 both penetrate through the inner cavities of the two slot holes 213 and extend to the outside of the box body 201. Limiting grooves 214 are opened on the front and rear sides of the top of the box body 201. The two fixing frames 208 both penetrate through the inner cavities of the two limiting grooves 214 and extend to the top of the box body 201.

[0035] By setting the protective pads 209, it can prevent the fixing frame 208 from directly contacting the microfluidic chip 3, thus avoiding the situation of leaving scratches on the surface and inside of the microfluidic chip 3. By setting the installation bolts 210, the overall fixing mechanism 2 can be disassembled and assembled, which is convenient for the maintenance of the components in the fixing mechanism 2. By setting the sliding grooves 211, the movement of the sliders 203 can be limited, preventing them from shifting during movement and thus affecting the fixing of the microfluidic chip 3. By setting the moving groove 212, the moving direction of the connecting frames 206 can be limited, preventing their directions from changing during movement and thus affecting the fixing effect of the microfluidic chip 3. By setting the slot holes 213, the movement of the pull rods 207 can be limited, preventing their directions from changing when the pull rods 207 pull the connecting frames 206 to move and thus affecting the fixing of the microfluidic chip 3. By setting the limiting grooves 214, the moving direction of the pull rods 207 can be limited, preventing them from shifting during movement and thus affecting the fixing effect of the microfluidic chip 3.

[0036] Usage method of a high-throughput Raman single-cell sorting device: It includes the following steps: S1: During use, open the connection door 5 and pull the pull rod 207 to move outward. The connection frame 206 and the slider 203 move synchronously to the left and right sides. At this time, the first rigid spring 204 is stretched and deformed, and at the same time, the second rigid spring 205 is also deformed under the extrusion of the slider 203. The fixing frame 208 moves synchronously with the connection frame 206 to the left and right sides and prepares for the fixing work of the microfluidic chip 3.

[0037] S2: Place the microfluidic chip 3 inside the fixing frame 208. Then, by releasing the pull rod 207, at this time, the second rigid spring 205 and the first rigid spring 204 lose the influence of force and deform. The first rigid spring 204 and the second rigid spring 205 respectively use their own tensions to make the slider 203 move inward. At this time, the slider 203 drives the connection frame 206 and the fixing frame 208 to move inward synchronously. The protective pads 209 on the surface of the fixing frame 208 are attached to the left and right sides of the microfluidic chip 3 and complete the fixing work on it.

[0038] S3: Through the extension of the cylinder 7, the lifting platform 8 drives the fixing mechanism 2 and the microfluidic chip 3 to move upward. When the microfluidic chip 3 is in a suitable position, inject the cell suspension into the microfluidic chip 3. Then, start the sorting work on the cells through the operation of the sorting device body 1.

[0039] When the present invention is working: Open the connection door 5 and pull the pull rod 207 to move outward. The connection frame 206 and the slider 203 move synchronously to the left and right sides. At this time, the first rigid spring 204 is stretched and deformed, and at the same time, the second rigid spring 205 is also deformed under the extrusion of the slider 203. The fixing frame 208 moves synchronously with the connection frame 206 to the left and right sides. Place the microfluidic chip 3 inside the fixing frame 208. Then, by releasing the pull rod 207, at this time, the second rigid spring 205 and the first rigid spring 204 lose the influence of force and deform. The first rigid spring 204 and the second rigid spring 205 respectively use their own tensions to make the slider 203 move inward. At this time, the slider 203 drives the connection frame 206 and the fixing frame 208 to move inward synchronously. The protective pads 209 on the surface of the fixing frame 208 are attached to the left and right sides of the microfluidic chip 3 and complete the fixing work on it. Through the extension of the cylinder 7, the lifting platform 8 drives the fixing mechanism 2 and the microfluidic chip 3 to move upward. When the microfluidic chip 3 is in a suitable position, inject the cell suspension into the microfluidic chip 3. Then, start the sorting work on the cells through the operation of the sorting device body 1.

[0040] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of various changes or substitutions, and these should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims described.

Claims

1. A high-throughput Raman single-cell sorting device, comprising a sorting device body (1) and a microfluidic chip (3), characterized in that, A connection door (5) is movably connected to the front side of the sorting device body (1) through a hinge. A control panel (4) is fixedly installed on the front side of the sorting device body (1). A partition (6) is fixedly installed in the inner cavity of the sorting device body (1). A cylinder (7) is fixedly installed at the bottom of the inner cavity of the sorting device body (1). A lifting platform (8) is fixedly installed at the top of the cylinder (7). The microfluidic chip (3) is located in the inner cavity of the sorting device body (1). A fixing mechanism (2) is arranged in the inner cavity of the sorting device body (1). The fixing mechanism (2) includes a box body (201). The box body (201) is located in the inner cavity of the sorting device body (1). Slide rods (202) are fixedly installed on both the front and rear sides of the inner cavity of the box body (201). Sliders (203) are slidably connected to the left and right sides of the surfaces of the two slide rods (202). First rigid springs (204) are fixedly connected to the inner sides of the four sliders (203). Second rigid springs (205) are fixedly connected to the outer sides of the four sliders (203). The outer sides of the four second rigid springs (205) are fixedly connected to the left and right sides of the inner cavity of the box body (201). Connecting frames (206) are fixedly installed on the inner sides of the four sliders (203). Pull rods (207) are fixedly installed on the outer sides of the two connecting frames (206). Fixing frames (208) are fixedly installed on the tops of the two connecting frames (206).

2. The high-throughput Raman single-cell sorting device according to claim 1, wherein: Protective pads (209) are fixedly installed on the inner sides of the two fixing frames (208). The two protective pads (209) are both attached to the left and right sides of the microfluidic chip (3), and the material of the protective pads (209) is rubber.

3. The high-throughput Raman single-cell sorting device according to claim 1, characterized in that: Mounting bolts (210) are threadedly connected to both the left and right sides of the box body (201). The bottoms of the four mounting bolts (210) are threadedly connected to the inner surface of the lifting platform (8).

4. A high-throughput Raman single-cell sorting device according to claim 1, characterized in that: Chute grooves (211) are opened on both the front and rear sides of the inner cavity of the box body (201). The outer sides of the four sliders (203) are slidably connected to the inner cavities of the two chute grooves (211).

5. The high-throughput Raman single-cell sorting device according to claim 1, wherein: A moving groove (212) is opened at the bottom of the inner cavity of the box body (201). The bottoms of the two connecting frames (206) are slidably connected to the inner cavity of the moving groove (212).

6. The high-throughput Raman single-cell sorting device according to claim 1, wherein: Slot holes (213) are opened on both the left and right sides of the inner cavity of the box body (201). The two pull rods (207) both penetrate through the inner cavities of the two slot holes (213) and extend to the outside of the box body (201).

7. A high-throughput Raman single-cell sorting device according to claim 1, characterized in that: Limit slots (214) are opened on both the front and rear sides of the top of the box body (201). The two fixing frames (208) both penetrate through the inner cavities of the two limit slots (214) and extend to the top of the box body (201).

8. The method of using a high-throughput Raman single-cell sorting device according to claim 1, characterized in that: Including the following steps: S1: When in use, open the connecting door (5) and pull the pull rod (207) to move outward. The connecting frame (206) and the slider (203) move synchronously to the left and right sides. At this time, the first rigid spring (204) is stretched and deformed. At the same time, the second rigid spring (205) is also deformed under the extrusion of the slider (203). The fixing frame (208) moves synchronously to the left and right sides with the connecting frame (206) and prepares for the fixing work of the microfluidic chip (3). S2: Place the microfluidic chip (3) inside the fixing frame (208). Then, by releasing the pull rod (207), at this time, the second rigid spring (205) and the first rigid spring (204) are deformed due to the loss of the influence of force. The first rigid spring (204) and the second rigid spring (205) respectively use their own tensions to make the slider (203) move inward. At this time, the slider (203) synchronously drives the connecting frame (206) and the fixing frame (208) to move inward. The protective pads (209) on the surface of the fixing frame (208) are attached to the left and right sides of the microfluidic chip (3) and complete the fixing work on it. S3: Through the extension of the cylinder (7), the lifting platform (8) drives the fixing mechanism (2) and the microfluidic chip (3) to move upward. When the microfluidic chip (3) is in the appropriate position, inject the cell suspension into the microfluidic chip (3). Then, start the cell sorting work by running the sorting device body (1).

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