Wuling ginseng cultivation method based on termitarium

Abstract

The invention discloses a xylaria nigripes cultivation method based on termite nests, and belongs to the technical field of xylaria nigripes cultivation, and the method comprises the following steps: step 1, cultivation of termite nests: termite species collection, termite cultivation, termite feeding, termite nesting, and termite nest transplanting: transplanting the termite nests into a field environment, and taking the termite nests when the termite nests grow to a diameter of 17-25 cm and a height of 7-10 cm; step 2, manufacturing a container: adding water into clay, stirring until the clay is sticky, kneading into the container, and putting the container into a plastic box; 3, cultivating xylaria nigripes: taking ant nests: finding the ant nests in the transplanting land parcels, and recovering the ant nests with viscous soil after one third of each nest is collected; the collected ant nests are brought back to culture points within 24 hours; extracting xylaria nigripes strains; and cultivating xylaria nigripes. According to the method, the termite species are manually collected, the termite nests are cultivated, and then field transplanting and termite nest collecting are conducted, so that factory cultivation of xylaria nigripes can be achieved, and the current situations that wild xylaria nigripes are low in yield, extremely difficult to collect and difficult to use in a large scale are made up.

Description

Technical Field

[0001] The present invention relates to the technical field of Wuling ginseng cultivation, and particularly relates to a method for cultivating Wuling ginseng based on termite nests. Background Art

[0002] Wuling ginseng is a sclerotium formed by the mycelium of the rare medicinal fungus Xylaria nigripes that grows deep underground on the abandoned nests of soil-dwelling termites. It has a sweet and neutral taste, is non-toxic, and enters the heart, liver, stomach, and bladder meridians. Wuling ginseng contains rich proteins, various amino acids required by the human body (mainly lysine, tryptophan, glutamic acid, γ-aminobutyric acid), sterols, polysaccharides, adenosine, minerals and trace elements such as calcium, magnesium, iron, and zinc, vitamins, and other components, as well as active components such as mannitol and proteoglycans. It has the effects of strengthening the spleen and removing dampness, calming the mind and tranquilizing the nerves, replenishing qi and strengthening the kidney, nourishing blood, etc., and can treat various diseases such as poor appetite due to spleen deficiency, excessive blood loss, gastroptosis, insomnia, and depression.

[0003] Wuling ginseng has extensive medicinal value and can be used to treat diseases such as neurasthenia, anxiety disorder, depression, benign prostatic hyperplasia, and irritable bowel syndrome in clinical applications.

[0004] However, generally, when collecting Wuling ginseng in the wild, it can only be collected from old abandoned termite nests that are several years old, resulting in low yields of wild Wuling ginseng and extremely difficult excavation. It is particularly difficult to use a large amount of Wuling ginseng.

[0005] Based on this, the present invention designs a method for cultivating Wuling ginseng based on termite nests to solve the above problems. Summary of the Invention

[0006] In view of the above-mentioned drawbacks of the prior art, the present invention provides a method for cultivating Wuling ginseng based on termite nests.

[0007] To achieve the above objectives, the present invention is realized through the following technical solutions:

[0008] A method for cultivating Wuling ginseng based on termite nests, the steps are as follows:

[0009] Step 1. Cultivate a termite nest, specifically:

[0010] Step 1.1. Collect termite species;

[0011] Step 1.2. Cultivate termites;

[0012] Step 1.3. Feed termites;

[0013] Step 1.4. Build a termite nest;

[0014] Step 1.5. Transplant the termite nest: Transplant the termite nest to the wild environment for growth;

[0015] Step 2. Make the container: Add water to the clay and stir until it becomes viscous, then knead it into a container. Place the container in a plastic box.

[0016] Step 3. Cultivate Wuling Ginseng:

[0017] Step A. Collect the ant nest: Locate the ant nest in the transplantation plot. After collecting a part from each nest, restore it with viscous soil. Bring the collected ant nests back to the cultivation site within 24 hours.

[0018] Step B. Extract the Wuling Ginseng strain.

[0019] Step C. Cultivate Wuling Ginseng: Weigh 46 - 53 parts of corn flour, 48 - 54 parts of wheat bran, 8 - 12 parts of glucose, 18 - 22 parts of oak sawdust, 0.5 - 1.3 parts of magnesium sulfate, 0.8 - 1.2 parts of potassium dihydrogen phosphate, and 1 - 3 parts of beef extract. After high-temperature sterilization and cooling, place them at the bottom of the container made in Step 2. Add 750 - 1100 parts of ant nests, inoculate with the Wuling Ginseng strain, seal the container with viscous soil. At the same time, spread fine soil on the bottom of the plastic box, cover the box with a lid, and cultivate in the cultivation room.

[0020] Furthermore, Step 1.1. Collect the ant species: From March to May, collect flying termites in the wild, pair them male and female, and put them into a plastic cup with soil in it for them to build nests and reproduce.

[0021] Furthermore, Step 1.2. Cultivate the termites: Put the paired ant species into a light-proof room with a constant temperature of 26°C and a humidity of 70% for cultivation. After 6 days, the termites lay eggs. After 45 days, the eggs hatch into larvae. After 20 days, the worker termites can go out for foraging.

[0022] Furthermore, Step 1.3. Feed the termites, specifically:

[0023] Step A. Make the feed: Divide the wood into blocks. According to 40 - 60 catties of wood blocks, add 15 - 22 catties of wheat bran, 2 - 4 catties of soybean cake, 0.5 - 1.2 catties of calcium carbonate, 0.3 - 1 catties of calcium hydroxide, 8 - 12 catties of sugarcane bagasse, 8 - 13 catties of corn cob, 1 - 3 catties of glucose, 0.5 - 1.3 catties of brown sugar, 1 - 3 catties of superphosphate, 0.1 - 0.5 catties of magnesium sulfate, and 0.2 - 0.5 catties of potassium dihydrogen phosphate. Put them into a sterilized bag, after high-temperature sterilization and cooling, inoculate with wood-decaying fungi. After cultivating for 18 - 22 days, it can be used for feeding.

[0024] Step B. Feeding method: Break the prepared feed into small pieces of 2.5 - 3.5 cm for the termites to freely feed on.

[0025] Furthermore, Step 1.4. The termites build nests: After the worker termites feed on the feed, they start to build ant nests, and the ant nest construction is completed in 60 - 70 days.

[0026] Further, in step 1.5, the environmental requirements in the wild are as follows: sunny or semi-sunny mountainous areas or under forests, with thick viscous soil; use a hole drill to drill holes with a diameter of 9 - 11 cm, a depth of 28 - 32 cm, and an angle of 42 - 47 degrees; the ant nest can be retrieved when it grows to a diameter of 17 - 25 cm and a height of 7 - 10 cm.

[0027] Further, in step two, making the container: Collect yellow clay or red clay, sieve it through a ten-mesh sieve, add 20 catties of water to 100 catties of soil, and stir it with a blender until it becomes viscous; knead the viscous soil into a container with a length of 50 - 70 cm, a width of 30 - 50 cm, and a height of 20 - 30 cm, and place the container in a plastic box with a length of 70 - 90 cm, a width of 40 - 60 cm, and a height of 30 - 50 cm.

[0028] Further, in step three, for step B, extraction of Wuling mushroom strains: Collect wild Wuling mushroom fruiting bodies, prepare test tube culture media. Calculated according to 80 test tubes with a diameter of 20 * 200 mm, take 18 - 23 grams of potato powder, 8 - 11 grams of mung bean powder, 4 - 6 grams of corn flour, 4 - 6 grams of wheat flour, 3 - 5 grams of magnesium sulfate, 3 - 5 grams of potassium dihydrogen phosphate, 3 - 5 grams of peptone, 3 - 5 grams of beef extract, 35 - 42 grams of glucose, 8 - 12 grams of agar, and 0.5 - 1.5 grams of vitamin B1, add 1000 ml of pure water, adjust the pH value to 8, fill each test tube with 12.5 ml, sterilize at high temperature and cool for later use; disinfect the Wuling mushroom with a 0.5% concentration of potassium permanganate solution, then disinfect it with 75% alcohol, then wash it with sterile water, then place it in a laminar flow hood and sterilize it with ultraviolet light, air-dry the surface moisture, extract 3 * 3 mm Wuling mushroom tissue and put it into the test tube for cultivation, keep the temperature at 24 - 27 °C, and after 24 - 26 days of cultivation, the Wuling mushroom strains will grow full.

[0029] Further, in step three, spread fine soil with a humidity of 45 - 55% in the plastic box.

[0030] Further, in step three, adjust the temperature of the cultivation room to 28 °C - 30 °C, control the humidity at 78 - 82%, and after 58 - 62 days of cultivation, 5 - 8 Wuling mushroom fruiting bodies with a length of 3 - 10 cm can grow, and the weight is between 228 - 355 grams.

[0031] To better achieve the purpose of the present invention, the present invention also provides

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) After the ant nest of the present invention is artificially cultivated and then transplanted into the wild environment, when it grows to a certain size, a part of the ant nest is retrieved and cultivated at the cultivation point for Wuling mushroom cultivation, which can ensure the stability of the number of ant nests.

[0033] (2) The method of collecting the ant nests in the present invention is a sustainable regeneration method. After taking one-third of the ant nests each time, the ant nests are restored to allow them to continue growing. On the one hand, it will not cause the death of termites due to taking the ant nests, and on the other hand, it is beneficial for continued collection next time.

[0034] (3) The present invention optimizes the components and proportions of the cultivation materials for Wuling ginseng (46 - 53 parts of corn flour, 48 - 54 parts of wheat bran, 8 - 12 parts of glucose, 18 - 22 parts of oak sawdust, 0.5 - 1.3 parts of magnesium sulfate, 0.8 - 1.2 parts of potassium dihydrogen phosphate, and 1 - 3 parts of beef extract), which is beneficial to improving the cultivation quality and yield of Wuling ginseng.

[0035] (4) The present invention optimizes the cultivation vessels for Wuling ginseng. Self-made clay containers are used, and the clay containers are placed in plastic boxes, and at the same time, the plastic boxes are filled with fine soil with a humidity of 45 - 55%. After 58 - 62 days of cultivation, 5 - 8 Wuling ginseng fruiting bodies with a length of 3 - 10 cm can grow, and the weight is 228 - 355 grams.

[0036] (5) By artificially collecting ant species and cultivating ant nests, and then transplanting them in the wild and collecting ant nests, the present invention can realize the industrialized cultivation of Wuling ginseng to make up for the situation of low yield, extremely difficult collection, and difficult large-scale use of wild Wuling ginseng.

[0037] (6) The Wuling ginseng cultivated by the method of the present invention has the advantages of good quality, high yield, and convenient collection.

[0038] (7) The cultivation period of Wuling ginseng by the method of the present invention is significantly shortened: The wild collection period: Wuling ginseng can only be produced under high temperature and high humidity from May to November every year. Using the method of the present invention: It can be cultivated all year round, and the cultivation period only takes about two months. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention, and those of ordinary skill in the art can obtain other drawings based on these drawings without creative efforts.

[0040] Figure 1 Wuling ginseng cultivated by the method of the present invention Figure 1 (Initial stage).

[0041] Figure 2 Wuling ginseng cultivated by the method of the present invention Figure 2 (Initial stage).

[0042] Figure 3 Wuling ginseng cultivated by the method of the present invention Figure 1 (Medium stage).

[0043] Figure 4 The Wuling ginseng cultivated by the method of the present invention Figure 2 (mid-term).

[0044] Figure 5 It is a comparison chart of the Wuling ginseng cultivated by the method of the present invention and the wild Wuling ginseng. Specific implementation mode

[0045] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the scope of protection of the present invention.

[0046] Embodiment 1: A method for cultivating Wuling ginseng based on termite nests, comprising the following steps:

[0047] Step 1. Cultivate termite nests, specifically:

[0048] Step 1.1. Collection of termite species: From March to May, collect flying termites in the wild, pair males and females, and put them into a plastic cup with a diameter of 9 cm and a height of 8 cm. Put 350 grams of soil with a humidity of 35% in the cup for them to build nests and reproduce.

[0049] Step 1.2. Cultivation of termites: Put the paired termite species into a light-proof room with a constant temperature of 26 °C and a humidity of 70% for cultivation; after 6 days of cultivation, the termites lay eggs, after 45 days of cultivation, the eggs hatch into larvae, and after 20 days of cultivation, the worker termites can go out to forage.

[0050] Step 1.3. Feeding of termites, specifically:

[0051] Step A. Preparation of the feeding material: Cut wood such as oak, pine, and fir into small pieces with a length of 2 cm, a width of 2 cm, and a height of 3 cm to obtain wood blocks; for every 50 catties of wood blocks, add 20 catties of wheat bran, 3 catties of soybean meal, 1 catty of calcium carbonate, 0.5 catty of calcium hydroxide, 10 catties of sugarcane bagasse, 10 catties of corn cob, 2 catties of glucose, 1 catty of brown sugar, 2 catties of superphosphate, 0.2 catty of magnesium sulfate, and 0.3 catty of potassium dihydrogen phosphate, put them into a sterilization bag of 17*35 cm, sterilize at 121 °C for 2 hours, and after cooling, inoculate with wood-decaying fungi (production of wood-decaying fungi: collect the fruiting bodies of semi-zhi fungi in the wild, extract tissue separation, carry out purification of the bacterial body, and transfer and cultivate in a nutrient solution to obtain), and after 20 days of cultivation, it can be fed.

[0052] Step B. Feeding method: Break the prepared feeding material into small pieces about 3 cm in size and put them into a plastic cup for termites to freely feed on.

[0053] Step 1.4, Termite nest building: After the worker termites feed on the feeding material, they start to build a termite nest, and the nest building is completed in 60 - 70 days.

[0054] Step 1.5, Termite nest transplantation: Transplant the cultivated termite nest into the wild environment. Environmental requirements: Sunny or semi - sunny mountainous areas or under the forest, and the soil is preferably thick viscous soil; Transplantation key points: Use a hole - drilling machine to drill a hole with a diameter of 10 cm, a depth of 30 cm, and an angle of 45 degrees. Invert the termite nest and place it at the bottom of the hole, then cover it with soil; After three years, when the termite nest grows to a diameter of 17 - 25 cm and a height of 7 - 10 cm, it can be used.

[0055] Step Two, Making containers: Collect yellow clay or red clay, sieve it through a 10 - mesh sieve. Add 20 catties of water to 100 catties of soil and stir it into a viscous state with an HWT25 - type mixer; Knead the viscous soil into a container with a length of 60 cm, a width of 40 cm, and a height of 25 cm, and place the container into a plastic box with a length of 80 cm, a width of 50 cm, and a height of 40 cm.

[0056] Step Three, Cultivating Wuling ginseng, specifically:

[0057] Step A, Taking the termite nest: Use an ultrasonic detector to accurately locate the termite nest in the termite transplantation plot, and open the termite nest. After collecting one - third of each nest, restore the termite nest with viscous soil; Bring the collected termite nest back to the cultivation point within 24 hours for Wuling ginseng cultivation.

[0058] Step B, Extracting Wuling ginseng strains: Collect wild Wuling ginseng fruiting bodies and bring them back to the laboratory. Prepare test - tube culture media. Calculated according to 80 test tubes with a diameter of 20 * 200 mm, take 20 grams of potato powder, 10 grams of mung bean powder, 5 grams of corn flour, 5 grams of wheat flour, 4 grams of magnesium sulfate, 4 grams of potassium dihydrogen phosphate, 4 grams of peptone, 4 grams of beef extract, 40 grams of glucose, 10 grams of agar, and 1 gram of vitamin B1, add 1000 ml of pure water, adjust the pH value to 8, and fill 12.5 ml into each test tube. After sterilizing at 121 °C for 30 minutes, take it out and cool it for standby; Disinfect the Wuling ginseng with a 0.5% concentration of potassium permanganate solution, then disinfect it with 75% alcohol, then wash it with sterile water, and then put it into a laminar flow hood for ultraviolet sterilization, air - dry the surface moisture, extract 3 * 3 mm of Wuling ginseng tissue and put it into the test tube for cultivation, keep the temperature at 26 °C, and the Wuling ginseng strains can grow full after 25 days of cultivation.

[0059] Step C, Method for cultivating Wuling mushroom: Weigh 50 grams of corn flour, 50 grams of wheat bran, 10 grams of glucose, 20 grams of oak sawdust, 1 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, and 2 grams of beef extract, put them into a sterilized bag, sterilize at a high temperature of 121 °C for 2 hours, after cooling, place them at the bottom of the container made in Step 2, add 1000 grams of termite nest, inoculate with Wuling mushroom strain, seal the container with viscous soil, and at the same time spread fine soil with a humidity of 50% in a plastic box, cover the plastic box with a lid, place it in a cultivation room, adjust the temperature of the cultivation room to 28 °C, control the humidity at 80%, and after 60 days of cultivation, 8 Wuling mushroom fruiting bodies with a length of 3 - 10 cm can grow, and the weight is between 255 - 350 grams.

[0060] Example 2: A method for cultivating Wuling mushroom based on termite nest, including the following steps:

[0061] Step 1, Cultivating termite nest, specifically:

[0062] Step 1.1, Collection of termite species: From March to May, collect flying termites in the wild, pair them male and female, put them into a plastic cup with a diameter of 9 cm and a height of 8 cm, and put 350 grams of soil with a humidity of 35% in the cup for them to build nests and reproduce.

[0063] Step 1.2, Cultivation of termites: Put the paired termite species into a light - proof room with a constant temperature of 26 °C and a humidity of 70% for cultivation; after 6 days of cultivation, the termites lay eggs, after 45 days of cultivation, the eggs hatch into larvae, and after 20 days of cultivation, the worker termites can go out for foraging.

[0064] Step 1.3, Feeding termites, specifically:

[0065] Step A, Preparation of feeding material: Cut woods such as oak, pine, and fir into small pieces with a length of 2 cm, a width of 2 cm, and a height of 3 cm to obtain wood blocks; according to 40 catties of wood blocks, add 22 catties of wheat bran, 2 catties of soybean meal, 1.2 catties of calcium carbonate, 0.3 catties of calcium hydroxide, 12 catties of sugarcane bagasse, 8 catties of corn cob, 3 catties of glucose, 0.5 catties of brown sugar, 3 catties of superphosphate, 0.1 catties of magnesium sulfate, and 0.5 catties of potassium dihydrogen phosphate, put them into a sterilized bag of 17 * 35 cm, sterilize at a high temperature of 121 °C for 2 hours, after cooling, inoculate with wood - decaying fungus (Preparation of wood - decaying fungus: Collect the fruiting bodies of Hemitelia semisupina in the wild, extract tissue separation, conduct bacterial body purification, and transfer and inoculate into a nutrient solution for cultivation to obtain), and after 18 days of cultivation, it can be used for feeding.

[0066] Step B, Feeding method: Break the prepared feeding material into small pieces about 2.5 cm in size and put them into a plastic cup for termites to freely feed on.

[0067] Step 1.4, Nest - building by termites: After the worker termites feed on the feeding material, they start to build a termite nest, and the nest - building is completed in 60 - 70 days.

[0068] Step 1.5, Ant nest transplantation: Transplant the cultivated ant nest into the wild environment. Environmental requirements: Sunny or semi-sunny mountainous areas or under forests, and the soil is preferably thick viscous soil. Transplantation key points: Use a hole drilling machine to drill a hole with a diameter of 11 cm, a depth of 32 cm, and an angle of 47 degrees. Invert the ant nest and place it at the bottom of the hole, then cover it with soil. After three years, when the ant nest grows to a diameter of 17 - 25 cm and a height of 7 - 10 cm, it can be used.

[0069] Step Two, Making the container: Collect yellow clay or red clay, sieve it through a 10-mesh sieve. Add 20 catties of water to 100 catties of soil and stir it into a viscous state with an HWT25 type mixer. Knead the viscous soil into a container with a length of 50 cm, a width of 30 cm, and a height of 20 cm, and place the container in a plastic box with a length of 70 cm, a width of 40 cm, and a height of 30 cm.

[0070] Step Three, Cultivating Wuling ginseng, specifically:

[0071] Step A, Taking the ant nest: Use an ultrasonic detector to accurately locate the ant nest in the termite transplantation plot, open the ant nest, and after collecting one-third of each nest, restore the ant nest with viscous soil. Bring the collected ant nest back to the cultivation point within 24 hours for Wuling ginseng cultivation.

[0072] Step B, Extracting Wuling ginseng strains: Collect wild Wuling ginseng fruiting bodies and bring them back to the laboratory. Prepare test tube culture media. Calculated according to 80 test tubes with a diameter of 20 * 200 mm, take 18 grams of potato powder, 11 grams of mung bean powder, 4 grams of corn flour, 6 grams of wheat flour, 3 grams of magnesium sulfate, 5 grams of potassium dihydrogen phosphate, 3 grams of peptone, 5 grams of beef extract, 35 grams of glucose, 12 grams of agar, and 0.5 grams of vitamin B1. Add 1000 ml of pure water, adjust the pH value to 8, and fill 12.5 ml into each test tube. After sterilizing at 121 °C for 30 minutes, take it out and cool for later use. Disinfect the Wuling ginseng with a 0.5% concentration of potassium permanganate solution, then disinfect it with 75% alcohol, then wash it with sterile water, and then place it in a clean bench for ultraviolet sterilization. Air-dry the water on the surface, extract 3 * 3 mm of Wuling ginseng tissue and put it into the test tube for cultivation. Keep the temperature at 24 °C, and after 26 days of cultivation, the Wuling ginseng strains can grow full.

[0073] Step C, Wuling ginseng cultivation method: Weigh 46 grams of corn flour, 54 grams of wheat bran, 8 grams of glucose, 22 grams of oak sawdust, 0.5 grams of magnesium sulfate, 1.2 grams of potassium dihydrogen phosphate, and 1 gram of beef extract, put them into a sterilized bag, sterilize at 121 °C for 2 hours, cool and place them at the bottom of the container made in Step Two. Add 1100 grams of ant nest, inoculate the Wuling ginseng strains, seal the container with viscous soil. At the same time, spread fine soil with a humidity of 45% in the plastic box, cover the plastic box with a lid, place it in a cultivation room, adjust the temperature of the cultivation room to 29 °C, control the humidity at 82%. After 58 days of cultivation, 5 Wuling ginseng fruiting bodies with a length of 3 - 10 cm can grow, and the weight is between 228 - 335 grams.

[0074] Example 3: A method for cultivating Wuling ginseng based on termite nests, comprising the following steps:

[0075] Step 1. Cultivate termite nests, specifically:

[0076] Step 1.1. Collection of termite species: In March - May, collect flying termites in the wild, pair males and females, and place them in a plastic cup with a diameter of 9 cm and a height of 8 cm. Put 350 grams of soil with a humidity of 35% in the cup for them to build nests and reproduce.

[0077] Step 1.2. Cultivation of termites: Put the paired termite species into a light - proof room with a constant temperature of 26°C and a humidity of 70% for cultivation; after 6 days of cultivation, the termites lay eggs, after 45 days of cultivation, the eggs hatch into larvae, and after 20 days of cultivation, the worker termites can go out for foraging.

[0078] Step 1.3. Feeding of termites, specifically:

[0079] Step A. Preparation of feed: Cut woods such as oak, pine, and fir into small pieces with a length of 2 cm, a width of 2 cm, and a height of 3 cm to obtain wood blocks; for every 60 catties of wood blocks, add 15 catties of wheat bran, 4 catties of soybean meal, 0.5 catty of calcium carbonate, 1 catty of calcium hydroxide, 8 catties of sugarcane bagasse, 13 catties of corn cob, 1 catty of glucose, 1.3 catties of brown sugar, 1 catty of superphosphate, 0.5 catty of magnesium sulfate, and 0.2 catty of potassium dihydrogen phosphate. Put them into a sterilization bag of 17 * 35 cm, sterilize at 121°C for 2 hours, and after cooling, inoculate with wood - decaying fungi (production of wood - decaying fungi: collect the fruiting bodies of Hemitelia semisupina in the wild, extract and isolate tissues, purify the mycelium, and transfer it into a nutrient solution for cultivation to obtain). After 22 days of cultivation, it can be used for feeding.

[0080] Step B. Feeding method: Break the prepared feed into small pieces about 3.5 cm in size and put them into a plastic cup for termites to freely feed on.

[0081] Step 1.4. Nest - building by termites: After the worker termites feed on the feed, they start to build termite nests, and the nest - building is completed in 60 - 70 days.

[0082] Step 1.5. Transplantation of termite nests: Transplant the cultivated termite nests into the wild environment. Environmental requirements: Sunny or semi - sunny mountains or under forests, and it is advisable to use thick viscous soil; Transplantation key points: Use a hole - drilling machine to drill a hole with a diameter of 9 cm, a depth of 28 cm, and an angle of 42 degrees. Invert the termite nest and put it at the bottom of the hole, then cover it with soil; After three years, when the termite nest grows to a diameter of 17 - 25 cm and a height of 7 - 10 cm, it can be taken for use.

[0083] Step 2. Making the container: Collect yellow clay or red clay, sieve it through a 10-mesh sieve, add 20 catties of water to 100 catties of soil, and stir it with an HWT25 type mixer until it becomes viscous; Knead the viscous soil into a container with a length of 70 cm, a width of 50 cm, and a height of 30 cm, and place the container in a plastic box with a length of 90 cm, a width of 60 cm, and a height of 50 cm.

[0084] Step 3. Cultivating Wuling ginseng, specifically:

[0085] Step A. Taking the ant nest: Use an ultrasonic detector to accurately locate the ant nest in the termite transplantation plot, open the ant nest, and after collecting one-third of each nest, restore the ant nest with viscous soil; Bring the collected ant nest back to the cultivation point within 24 hours for Wuling ginseng cultivation.

[0086] Step B. Extracting the Wuling ginseng strain: Collect the wild Wuling ginseng fruit bodies, bring them back to the laboratory, prepare the test tube culture medium. Calculated according to 80 test tubes with a diameter of 20*200 mm, take 23 grams of potato powder, 8 grams of mung bean powder, 6 grams of corn flour, 4 grams of wheat flour, 5 grams of magnesium sulfate, 3 grams of potassium dihydrogen phosphate, 5 grams of peptone, 3 grams of beef extract, 42 grams of glucose, 8 grams of agar, and 1.5 grams of vitamin B1, add 1000 ml of pure water, adjust the pH value to 8, fill 12.5 ml into each test tube, sterilize at 121 °C for 30 minutes, then take it out and cool it for standby; Disinfect the Wuling ginseng with a 0.5% concentration of potassium permanganate solution, then disinfect it with 75% alcohol, then wash it with sterile water, then place it in a clean bench and sterilize it with ultraviolet light, air-dry the water on the surface, extract 3*3 mm of Wuling ginseng tissue and put it into the test tube for cultivation, keep the temperature at 27 °C, and the Wuling ginseng strain can grow full after 24 days of cultivation.

[0087] Step C. Cultivation method of Wuling ginseng: Weigh 53 grams of corn flour, 48 grams of wheat bran, 12 grams of glucose, 18 grams of oak sawdust, 1.3 grams of magnesium sulfate, 0.8 grams of potassium dihydrogen phosphate, and 3 grams of beef extract, put them into a sterilized bag, sterilize at 121 °C for 2 hours, cool it and put it at the bottom of the container made in Step 2, add 750 grams of ant nest, inoculate the Wuling ginseng strain, seal the container with viscous soil, and at the same time spread fine soil with a humidity of 55% in the plastic box, cover the plastic box with a lid, place it in the cultivation room, adjust the temperature of the cultivation room to 30 °C, control the humidity at 78%, and after 62 days of cultivation, 6 Wuling ginseng fruit bodies with a length of 3 - 10 cm can grow, and the weight is between 231 - 355 grams.

[0088] The present invention has the following technical effects:

[0089] (1) Although there are currently related technologies for cultivating Wuling ginseng using ant nests, they use pure wild ant nests, and their quantity is unstable. The ant nests of the present invention are artificially cultivated and then transplanted into the wild environment. After growing to a certain size, a part of the ant nests is retrieved and used for cultivating Wuling ginseng at the cultivation site, which can ensure the stability of the quantity of ant nests.

[0090] (2) The method for retrieving ant nests in the present invention is a sustainable regeneration method. After taking one-third each time, the ant nests are restored and allowed to continue growing. On the one hand, it will not cause the death of termites due to taking ant nests, and on the other hand, it is beneficial for taking them again next time.

[0091] (3) The present invention optimizes the components and ratios of the cultivation materials for cultivating Wuling ginseng (46 - 53 parts of corn flour, 48 - 54 parts of wheat bran, 8 - 12 parts of glucose, 18 - 22 parts of oak sawdust, 0.5 - 1.3 parts of magnesium sulfate, 0.8 - 1.2 parts of potassium dihydrogen phosphate, and 1 - 3 parts of beef extract), which is beneficial to improving the cultivation quality and yield of Wuling ginseng;

[0092] (4) The present invention optimizes the cultivation utensils for cultivating Wuling ginseng. Self-made clay containers are used, and the clay containers are placed in plastic boxes. At the same time, the plastic boxes are filled with fine soil with a humidity of 45 - 55%. After 58 - 62 days of cultivation, 5 - 8 Wuling ginseng fruiting bodies with a length of 3 - 10 cm can grow, and the weight is 228 - 355 grams.

[0093] (5) The present invention can realize the industrialized cultivation of Wuling ginseng by artificially collecting ant species and cultivating ant nests, then transplanting them in the wild and collecting ant nests, so as to make up for the current situation of low yield, extremely difficult collection, and difficult large-scale use of wild Wuling ginseng.

[0094] (6) The Wuling ginseng cultivated by the method of the present invention has the advantages of good quality, high yield, and convenient collection.

[0095] (7) The cultivation period of Wuling ginseng by the method of the present invention is significantly shortened: The wild collection period: Wuling ginseng can only be produced under high temperature and high humidity from May to November every year. Using the method of the present invention: It can be cultivated throughout the year, and the cultivation period only takes about two months.

[0096] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements will not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for cultivating Wuling ginseng based on termite nests, characterized in that, The steps are as follows: Step 1: Cultivate termite nests, specifically: Step 1.1: Collect termite species; Step 1.2: Cultivate termites; Step 1.3: Feed termites; Step 1.4: Build termite nests; Step 1.5: Transplant termite nests: Transplant the termite nests to the wild environment for growth; Step 2: Make containers: Mix clay with water until it becomes viscous, knead it into containers, and place the containers in a plastic box; Step 3: Cultivate Wuling ginseng: Step A: Obtain termite nests: Locate termite nests in the transplanted plot. After collecting a part from each nest, restore it with viscous soil; Bring the collected termite nests back to the cultivation site within 24 hours; Step B: Extract Wuling ginseng strains; Step C: Cultivate Wuling ginseng: Weigh 46 - 53 parts of corn flour, 48 - 54 parts of wheat bran, 8 - 12 parts of glucose, 18 - 22 parts of oak sawdust, 0.5 - 1.3 parts of magnesium sulfate, 0.8 - 1.2 parts of potassium dihydrogen phosphate, and 1 - 3 parts of beef extract. After high-temperature sterilization and cooling, place them at the bottom of the container made in Step 2. Add 750 - 1100 parts of termite nests, inoculate with Wuling ginseng strains, seal the container with viscous soil. At the same time, spread fine soil in the plastic box, cover the plastic box with a lid, and cultivate in a cultivation room.

2. The method for cultivating Wuling ginseng based on termite nests according to claim 1, characterized in that, Step 1.1: Collect termite species: From March to May, collect flying termites in the wild, pair males and females, put them into plastic cups, and place soil in the cups for them to build nests and reproduce.

3. The cultivation method of Wuling ginseng based on termite nests according to claim 1, characterized in that, Step 1.2: Cultivate termites: Put the paired termite species into a light-proof room with a constant temperature of 26°C and a humidity of 70% for cultivation; After 6 days of cultivation, termites lay eggs. After 45 days of cultivation, the eggs hatch into larvae. After 20 days of cultivation, worker termites can go out for foraging.

4. The method for cultivating Xylaria nigripes based on termite nests according to claim 1, wherein, Step 1.3: Feed termites, specifically: Step A: Make feeding materials: Divide the wood into blocks; According to 40 - 60 catties of wood blocks, add 15 - 22 catties of wheat bran, 2 - 4 catties of soybean meal, 0.5 - 1.2 catties of calcium carbonate, 0.3 - 1 catties of calcium hydroxide, 8 - 12 catties of sugarcane bagasse, 8 - 13 catties of corn cobs, 1 - 3 catties of glucose, 0.5 - 1.3 catties of brown sugar, 1 - 3 catties of superphosphate, 0.1 - 0.5 catties of magnesium sulfate, and 0.2 - 0.5 catties of potassium dihydrogen phosphate. Load them into a sterilized bag, after high-temperature sterilization and cooling, inoculate with wood-rotting fungi. After 18 - 22 days of cultivation, they can be used for feeding; Step B: Feeding method: Break the prepared feeding materials into small pieces of 2.5 - 3.5 cm for termites to freely feed on.

5. The cultivation method of Xylaria nigripes based on termite nests according to claim 1, characterized in that Step 1.4: Build termite nests: After worker termites feed on the feeding materials, they start to build termite nests, and the nest construction is completed in 60 - 70 days.

6. The method for cultivating Wuling ginseng based on termite nests according to claim 1, characterized in that, In Step 1.5, the requirements for the wild environment are: sunny or semi-sunny mountains or under forests, and the soil is thick viscous soil; Use a hole drill to drill holes with a diameter of 9 - 11 cm, a depth of 28 - 32 cm, and an inclination angle of 42 - 47 degrees; The termite nests can be taken when they grow to a diameter of 17 - 25 cm and a height of 7 - 10 cm.

7. The method for cultivating Wuling ginseng based on termite nests according to claim 1, characterized in that, Step 2: Make containers: Collect yellow clay or red clay, sieve it through a ten-mesh sieve, add water to the clay and stir it with a mixer until it becomes viscous; Knead the viscous soil into containers with a length of 50 - 70 cm, a width of 30 - 50 cm, and a height of 20 - 30 cm, and place the containers in a plastic box with a length of 70 - 90 cm, a width of 40 - 60 cm, and a height of 30 - 50 cm.

8. The cultivation method of Wuling ginseng based on termite nests according to claim 1, characterized in that, In Step 3, Step B: Extraction of Wuling mushroom strains: Collect wild Wuling mushroom fruit bodies, and prepare test tube culture media. Calculated according to 80 test tubes with a diameter of 20×200 mm, take 18 - 23 grams of potato powder, 8 - 11 grams of mung bean powder, 4 - 6 grams of corn flour, 4 - 6 grams of wheat flour, 3 - 5 grams of magnesium sulfate, 3 - 5 grams of potassium dihydrogen phosphate, 3 - 5 grams of peptone, 3 - 5 grams of beef extract, 35 - 42 grams of glucose, 8 - 12 grams of agar, and 0.5 - 1.5 grams of vitamin B1. Add 1000 ml of pure water, adjust the pH value to 8, and fill 12.5 ml in each test tube. After high-temperature sterilization and cooling, it is reserved for use; Disinfect the Wuling mushroom with a 0.5% potassium permanganate solution, then disinfect it with 75% alcohol, then wash it with sterile water, and then place it in a laminar flow hood for ultraviolet sterilization. Air-dry the moisture on the surface, extract 3×3 mm Wuling mushroom tissue and put it into the test tube for cultivation, keep the temperature at 24 - 27°C, and after 24 - 26 days of cultivation, the Wuling mushroom strains will grow fully.

9. The method for cultivating Wuling ginseng based on termite nests according to claim 1, characterized in that, In Step 3, spread fine soil with a humidity of 45 - 55% in a plastic box.

10. The cultivation method of Xylaria nigripes based on termite nests according to any one of claims 1 to 9, characterized in that, In Step 3, adjust the temperature of the cultivation room to 28°C - 30°C, control the humidity at 78 - 82%, and after 58 - 62 days of cultivation, 5 - 8 Wuling mushroom fruit bodies with a length of 3 - 10 cm can grow, and the weight is 228 - 355 grams.

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