A natural mixed feed additive and its preparation method

Abstract

This application relates to the field of feed formulation technology, specifically disclosing a natural mixed feed additive and its preparation method. The natural mixed feed additive comprises the following raw materials in parts by weight: 4-7 parts vitamin E, 5-15 parts β-carotene, 10-16 parts soybean oil, 20-35 parts deionized water, 5-8 parts modified probiotic microcapsules, and 1-5 parts emulsifier. The modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics. The preparation method is as follows: 10-16 parts soybean oil are heated to 35-45°C, then 4-7 parts vitamin E and 5-15 parts β-carotene are added and stirred; 1-5 parts emulsifier are added to 20-35 parts deionized water, heated to 45-55°C and stirred, then slowly added to the mixture obtained in step S1; 5-8 parts modified probiotic microcapsules are added, and the mixture is stirred at 100-200 r / min for 8-12 min. The natural mixed feed additive of this application has the advantage of being able to improve the shortcomings of feed additives in reducing intestinal diseases in animals.

Description

Technical Field

[0001] This application relates to the field of feed formulation technology, and more specifically, to a natural mixed feed additive and its preparation method. Background Technology

[0002] As the material foundation for the development of the livestock industry, the dependence on feed is gradually increasing with the intensification of livestock farming. In order to meet the needs of animals for nutrients and physicochemical balance during growth and development, a small amount of additives, namely feed additives, are added to feed production. Feed additives can balance the nutritional components of feed, promote animal growth, and prevent disease. At the same time, the development of related industries faces many new challenges in the safety of feed. In recent years, the state has tightened the management and use of feed additives and raised the threshold. These new requirements have become the driving force for the further development of my country's feed additive industry.

[0003] Animals are typically weaned 3-4 weeks after birth, such as piglets. Early weaning exposes the underdeveloped intestines to indigestible solid feed prematurely, and the transfer to a new environment can cause post-weaning stress in piglets, leading to intestinal oxidative stress, gut microbiota imbalance, and consequently, intestinal damage and persistent inflammation. Intestinal damage and inflammation are the main causes of diarrhea and impaired growth performance in piglets. To prevent weaning diarrhea, adding antibiotic feed additives to weaned piglet feed has become a routine practice in animal husbandry. However, in recent years, the problem of antibiotic resistance in pathogenic bacteria has been intensifying, and the widespread use of antibiotics in animal husbandry has caused serious environmental pollution. Most regions worldwide have introduced relevant policies to strictly limit the use of antibiotics in animal husbandry. Therefore, there is an urgent need for a feed additive that can replace antibiotics to reduce intestinal diseases in animals. Summary of the Invention

[0004] In order to improve the shortcomings of feed additives in reducing intestinal diseases in animals, this application provides a natural mixed feed additive and its preparation method.

[0005] This application provides a natural mixed feed additive and its preparation method, which adopts the following technical solution:

[0006] In a first aspect, this application provides a natural mixed feed additive comprising the following raw materials in parts by weight: 4-7 parts vitamin E, 5-15 parts β-carotene, 10-16 parts soybean oil, 20-35 parts deionized water, 5-8 parts modified probiotic microcapsules, and 1-5 parts emulsifier, wherein the modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics.

[0007] The long-term use of antibiotics in livestock and poultry feed will increase the number of drug-resistant strains of pathogens, and normal strains will also develop drug resistance, thus affecting the effectiveness of drug treatment. Moreover, drug-resistant bacteria can be transmitted to humans through the food chain, threatening the control of human diseases. At the same time, antibiotics can enter the environment through animal excrement, thereby disrupting the ecological balance and polluting water sources.

[0008] Vitamin E neutralizes free radicals, protecting intestinal cells from free radical damage and maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa acts as a physical barrier, making it difficult for pathogens to invade, thus reducing the risk of intestinal infection. Vitamin E can regulate animal immune function, enhance local intestinal immune response, and improve resistance to intestinal pathogens. Beta-carotene can be converted into vitamin A in animals, promoting the regeneration and repair of intestinal mucosal epithelial cells and maintaining the integrity of the intestinal barrier. Intact intestinal epithelial cells can better resist the invasion of pathogens, reducing the occurrence of intestinal diseases. Furthermore, beta-carotene has certain antioxidant and immunomodulatory effects, enhancing intestinal immune function, promoting the growth of beneficial intestinal microorganisms, inhibiting the release of pro-inflammatory factors, and alleviating intestinal inflammation. Soybean oil provides animals with energy and essential fatty acids, promotes the stability of intestinal cell membrane structure, and also preserves the absorption and utilization of vitamin E and beta-carotene.

[0009] Meanwhile, probiotics can colonize the intestines, competing with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids and bacteriocins. These substances can lower the intestinal pH value, inhibit the growth of pathogens, and also have a certain antibacterial effect. The microencapsulation structure can protect probiotics from being destroyed when passing through harsh environments such as stomach acid and bile, ensuring that probiotics can successfully reach the intestines and exert their effects. At the same time, during processing and storage, vitamin E can neutralize free radicals, reduce oxidative stress damage to probiotics, and maintain their activity. Probiotics can activate gut-associated lymphoid tissue and promote the production of secretory immunoglobulin A, working with vitamin E to build a systemic immune defense.

[0010] Preferably, the modified probiotic microcapsules comprise the following raw materials in parts by weight: 1-2 parts Bifidobacterium, 3-5 parts sodium alginate-pectin composite microencapsulated probiotics.

[0011] Because Bifidobacteria can lower the pH of the intestine by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, making it difficult for harmful bacteria to colonize the intestine and thus reducing the risk of intestinal infection, sodium alginate-pectin complex microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the intestinal flora structure, and improve the stability and health of the intestinal microecology.

[0012] Preferably, the modified probiotic microcapsules are prepared by: activating 1-2 parts of Bifidobacterium by culturing in liquid MRS medium at 35-39℃ for 16-20h, and then placing 3-5 parts of sodium alginate-pectin composite microencapsulated probiotics into MRS medium for secondary fermentation to obtain modified probiotic microcapsules.

[0013] Because suitable temperature and culture time allow Bifidobacteria to grow and multiply fully, their metabolic activity is enhanced, enabling them to quickly exert their effects after entering the animal's intestines. This better regulates the balance of intestinal flora and inhibits the growth of harmful bacteria. Simultaneously, the secondary fermentation of the sodium alginate-pectin microencapsulated probiotics allows the probiotics inside the microcapsules to obtain more sufficient nutrients, promoting their growth and metabolism. Furthermore, during the secondary fermentation process, Bifidobacteria and the sodium alginate-pectin microencapsulated probiotics interact. Bifidobacteria can utilize the relatively stable microenvironment created by the microencapsulated probiotics to better perform their functions, while the microencapsulated probiotics receive more favorable survival conditions due to the metabolic products of Bifidobacteria. The synergistic effect of both enhances the improvement of intestinal health in animals.

[0014] Preferably, the sodium alginate-pectin composite microencapsulated probiotics include the following raw materials: 2-4 ml sodium alginate-pectin composite solution, 1-3 ml concentrated bacterial solution, 0.3-0.7 g calcium carbonate, 6-14 ml deionized water, 15-25 ml liquid paraffin, and 0.5-1.5 ml glacial acetic acid.

[0015] Since sodium alginate and pectin are both natural high-molecular polymers, the composite solution formed by the two has good film-forming properties and biocompatibility. It can form a protective film on the surface of probiotics, making them less susceptible to erosion by adverse external environments such as stomach acid and bile. This allows probiotics to reach the intestines smoothly and exert their effects. At the same time, this composite film can control the release rate of probiotics, allowing them to be released slowly in the intestines, prolonging the duration of action. The concentrated bacterial solution provides a sufficient number of probiotics for microencapsulation, ensuring that there are enough active bacteria in the animal's intestines to regulate the balance of intestinal flora, reduce the growth of harmful bacteria, and enhance intestinal immunity, thereby improving the efficacy of microencapsulated probiotics.

[0016] Preferably, the preparation method of the sodium alginate-pectin composite microencapsulated probiotics is as follows: Weigh 0.3-0.7g of calcium carbonate, dissolve it in 6-14ml of deionized water, and stir it evenly with 2-4ml of sodium alginate-pectin composite solution. Then add 1-3ml of concentrated bacterial solution and mix evenly. Subsequently, disperse it in 15-25ml of liquid paraffin and stir at 350-450r / min for 4-6min. Then add 0.5-1.5ml of glacial acetic acid and react for 8-12min. Finally, rinse with deionized water 2-4 times to obtain sodium alginate-pectin composite microencapsulated probiotics.

[0017] The method for preparing concentrated bacterial solution is as follows: the activated bacterial solution is centrifuged at 3500-4500 r / min for 8-12 min, washed with physiological saline 1-3 times, and the washed bacterial solution is resuspended in physiological saline to obtain concentrated bacterial solution.

[0018] Preferably, the sodium alginate-pectin composite solution comprises the following raw materials: 0.1-0.3g naringin, 26-34ml deionized water, 0.3-0.5g pectin, and 0.6-1g sodium alginate.

[0019] Because naringin possesses antioxidant, anti-inflammatory, neuroprotective, anti-obesity, and anti-diabetic bioactivities, its antioxidant properties in the compound solution can protect probiotics from oxidative damage and maintain their activity. Simultaneously, it exhibits effective broad-spectrum antibacterial activity, acting against bacteria such as Escherichia coli, Staphylococcus aureus, and harmless Listeria, as well as various viruses and fungi. This helps inhibit the growth of harmful microorganisms in feed, further ensuring the intestinal health of animals.

[0020] Preferably, the preparation method of the sodium alginate-pectin composite solution is as follows: Weigh 0.1-0.3g of naringin and add it to 18-22ml of deionized water. Stir magnetically at 55-65℃ and 350-450r / min for 25-35min. Then add 0.3-0.5g of pectin and continue stirring for 0.5-1.5h to obtain a mixed solution. Separately, take 0.6-1g of sodium alginate and add it to 8-12ml of deionized water. Stir magnetically at 20-30℃ and 350-450r / min for 0.5-1.5h to dissolve the alginate. Then transfer the dissolved sodium alginate solution to the mixed solution and continue stirring at 350-450r / min for 0.5-1.5h. Then rapidly cool in a water bath at 15-25℃ for 25-35min and remove air bubbles from the solution by ultrasonication.

[0021] Secondly, this application provides a method for preparing a natural mixed feed additive, which adopts the following technical solution:

[0022] A method for preparing a natural mixed feed additive includes the following steps:

[0023] S1: Heat 10-16 parts soybean oil to 35-45℃, then add 4-7 parts vitamin E and 5-15 parts beta-carotene, and stir.

[0024] S2: Add 1-5 parts of emulsifier to 20-35 parts of deionized water, heat to 45-55℃ and stir, then slowly add to the mixture obtained in S1;

[0025] S3: Add 5-8 parts of modified probiotic microcapsules and stir at 100-200 r / min for 8-12 min.

[0026] In summary, this application has the following beneficial effects:

[0027] 1. Vitamin E neutralizes free radicals, protecting intestinal cells from free radical damage and maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa acts as a physical barrier, making it difficult for pathogens to invade, thus reducing the risk of intestinal infection. Vitamin E can regulate animal immune function, enhance local intestinal immune response, and improve resistance to intestinal pathogens. β-carotene can be converted into vitamin A in animals, promoting the regeneration and repair of intestinal mucosal epithelial cells and maintaining the integrity of the intestinal barrier. Intact intestinal epithelial cells can better resist pathogen invasion, reducing the occurrence of intestinal diseases. β-carotene also has certain antioxidant and immunomodulatory effects, enhancing intestinal immune function, promoting the growth of beneficial intestinal microorganisms, inhibiting the release of pro-inflammatory factors, and alleviating intestinal inflammation. Soybean oil provides animals with energy and essential fatty acids, promotes the stability of intestinal cell membrane structure, and also preserves the absorption and utilization of vitamin E and β-carotene.

[0028] Meanwhile, probiotics can colonize the intestines, competing with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids and bacteriocins. These substances can lower the intestinal pH value, inhibit the growth of pathogens, and also have a certain antibacterial effect. The microencapsulation structure can protect probiotics from being destroyed when passing through harsh environments such as stomach acid and bile, ensuring that probiotics can successfully reach the intestines and exert their effects. At the same time, during processing and storage, vitamin E can neutralize free radicals, reduce oxidative stress damage to probiotics, and maintain their activity. Probiotics can activate gut-associated lymphoid tissue and promote the production of secretory immunoglobulin A, working with vitamin E to build a systemic immune defense.

[0029] 2. Because Bifidobacteria can lower the pH value of the intestine by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, it makes it difficult for harmful bacteria to colonize in the intestine, thus reducing the risk of intestinal infection. Sodium alginate-pectin complex microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the intestinal flora structure, and improve the stability and health of the intestinal microecology.

[0030] 3. Due to suitable temperature and culture time, Bifidobacteria can grow and multiply fully, and their metabolic activity is enhanced. Therefore, after entering the animal intestine, they can quickly exert their effects, better regulate the balance of intestinal flora, and inhibit the growth of harmful bacteria. At the same time, the sodium alginate-pectin complex microencapsulated probiotics undergo secondary fermentation, which allows the probiotics inside the microcapsules to obtain more nutrients, promoting their growth and metabolism. During the secondary fermentation process, Bifidobacteria and sodium alginate-pectin complex microencapsulated probiotics can interact. Bifidobacteria can utilize the relatively stable microenvironment created by the microencapsulated probiotics to better perform their functions, while the microencapsulated probiotics obtain more favorable survival conditions due to the metabolic products of Bifidobacteria. The two work synergistically to enhance the improvement effect on the animal's intestinal health. Detailed Implementation

[0031] The present application will be further described in detail below with reference to Examples 1-10 and Comparative Examples 1-2.

[0032] raw material

[0033] Vitamin E CAS: 2074-53-5; β-carotene CAS: 301150-50-5; Soybean oil CAS: 8001-22-7; Deionized water CAS: 7732-18-5; Emulsifier lecithin CAS: 8002-43-5; Bifidobacterium Shanghai Jiadeer Chemical Technology Co., Ltd.; Calcium carbonate CAS: 471-34-1; Liquid paraffin CAS: 8042-47-5; Glacial acetic acid CAS: 64-19-7; Naringin CAS: 10236-47-2; Pectin Huaibei Kailu Biotechnology Co., Ltd.; Sodium alginate Lianyungang Tiantian Seaweed Industry Co., Ltd.

[0034] Example 1

[0035] A natural mixed feed additive, comprising the following ingredients: 5.5g vitamin E, 10g β-carotene, 13ml soybean oil, 27.5ml deionized water, 6.5g modified probiotic microcapsules, and 3g lecithin.

[0036] Specifically, the preparation method of natural mixed feed additives includes the following steps:

[0037] S1: Activate 1.5 ml of Bifidobacterium by culturing it in liquid MRS medium at 37°C for 18 h;

[0038] S2: Weigh 0.2g of naringin and add it to 20ml of deionized water. Stir magnetically at 60℃ and 400r / min for 30min. Then add 0.4g of pectin and continue stirring for 1h to obtain a mixed solution. Separately, take 0.8g of sodium alginate and add it to 10ml of deionized water. Stir magnetically at 25℃ and 400r / min for 1h to dissolve it. Then transfer the dissolved sodium alginate solution to the mixed solution and continue stirring at 400r / min for 1h. Then rapidly cool it in a 20℃ water bath for 30min and remove the air bubbles in the solution by sonication to obtain a sodium alginate-pectin composite solution.

[0039] S3: Weigh 0.5g of calcium carbonate, dissolve it in 10ml of deionized water, and stir it evenly with 3ml of sodium alginate-pectin composite solution. Then add 2ml of concentrated bacterial solution and mix evenly. Then disperse it in 20ml of liquid paraffin and stir for 5min at 400r / min. Then add 1ml of glacial acetic acid and react for 10min. Finally, rinse 3 times with deionized water to obtain sodium alginate-pectin composite microencapsulated probiotics.

[0040] The method for preparing concentrated bacterial solution is as follows: the activated bacterial solution is centrifuged at 4000 r / min for 10 min, washed twice with physiological saline, and the washed bacterial solution is resuspended in physiological saline to obtain concentrated bacterial solution.

[0041] S4: 4g of sodium alginate-pectin composite microencapsulated probiotics were placed in MRS medium for secondary fermentation to obtain modified probiotic microcapsules.

[0042] S5: Heat 13ml of soybean oil to 35-45℃, then add 5.5g of vitamin E and 10g of beta-carotene, and stir.

[0043] S6: Add 3g of lecithin to 27.5ml of deionized water, heat to 50℃ and stir, then slowly add to the mixture obtained in S5;

[0044] S7: Add 6.5g of modified probiotic microcapsules and stir at 150r / min for 10min.

[0045] Example 2-Example 3

[0046] The difference from Example 1 is that the amount of each component added in the natural mixed feed additive is different, as shown in Table 1.

[0047] Table 1. Dosage of each component in the natural mixed feed additives used in Examples 1-3

[0048] Example 1 Example 2 Example 3 Vitamin E 5.5g 4g 7g beta-carotene 10g 15g 5g soybean oil 13ml 10ml 16ml Deionized water 27.5ml 20ml 35ml Modified probiotic microcapsules 6.5g 8g 5g Lecithin 3g 5g 1g

[0049] Example 4

[0050] The difference from Example 1 is that the modified probiotic microcapsules were replaced with an equal amount of probiotics.

[0051] Examples 5-6

[0052] The difference from Example 1 is that the amount of each component added to the modified probiotic microcapsule is different, as shown in Table 2.

[0053] Table 2. Quantities of each component added to the modified probiotic microcapsules in Examples 1 and 5-6.

[0054] Example 1 Example 5 Example 6 Bifidobacteria 1.5ml 1ml 2ml Sodium alginate-pectin complex microencapsulated probiotics 4g 5g 3g

[0055] Examples 7-8

[0056] The difference from Example 1 is that the amount of each component added to the sodium alginate-pectin complex microencapsulated probiotic is different, as shown in Table 3.

[0057] Table 3. Addition amounts of each component in sodium alginate-pectin microencapsulated probiotics in Examples 1 and 7-8.

[0058] Example 1 Example 7 Example 8 Sodium alginate-pectin complex solution 3ml 4ml 2ml Concentrated bacterial solution 2ml 3ml 1ml Calcium carbonate 0.5g 0.3g 0.7g Deionized water 10ml 6ml 14ml Liquid paraffin 20ml 15ml 25ml glacial acetic acid 1ml 1.5ml 0.5ml

[0059] Examples 9-10

[0060] The difference from Example 1 is that the amount of each component added to the sodium alginate-pectin composite solution is different, as shown in Table 4.

[0061] Table 4. Amounts of each component added to the sodium alginate-pectin composite solution in Examples 1 and 9-10.

[0062] Example 1 Example 9 Example 10 Naringin 0.2g 0.1g 0.3g Deionized water 30ml 34ml 26ml pectin 0.4g 0.3g 0.5g Sodium alginate 0.8g 1g 0.6g

[0063] Comparative Example 1

[0064] The difference from Example 1 is that the modified probiotic microcapsules were replaced with an equal amount of antibiotics.

[0065] Comparative Example 2

[0066] The difference from Example 1 is that vitamin E is no longer added.

[0067] Performance testing

[0068] I. Growth Performance Test

[0069] 220 healthy weaned piglets of similar birth date and weight at 35 days old were selected and divided into 11 groups of 20 piglets each. The natural mixed feed additives prepared in Examples 1-10 and Comparative Example 1 were added to the diets of the 11 groups. The amount of natural feed additives added was 1.8% of the weight of the piglets' diet. The piglets were fed continuously for 30 days, with 6 feedings per day, twice a day (morning, noon and evening).

[0070] Before the experiment, the fasting net weight of each group of piglets was recorded in the morning, and the average weight was used as the initial weight. After the experiment, the fasting net weight of the piglets in the morning was recorded, and the average weight was used as the final weight. At the same time, the diarrhea rate of the piglets' health status was recorded and the average value was taken. The experimental results are shown in Table 5.

[0071] The diarrhea rate is calculated as follows: (Cumulative number of days of diarrhea in piglets / Cumulative number of days of feeding in the whole group of piglets) × 100%.

[0072] Table 5. Growth performance test results of Examples 1-10 and Comparative Examples 1-2

[0073]

[0074]

[0075] Based on Example 1 and Comparative Example 1 and Table 5, it can be seen that although the average daily weight gain of Comparative Example 1 is higher than that of Example 1, the diarrhea rate of Comparative Example 1 is significantly increased. This indicates that compared with adding antibiotics, adding modified probiotic microcapsules can effectively reduce the diarrhea rate of piglets, thereby improving the intestinal health of piglets.

[0076] The reason for this is that probiotics can colonize the intestines and compete with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids and bacteriocins. These substances can lower the pH value of the intestines, inhibit the growth of pathogens, and also have a certain antibacterial effect. The structure of microcapsules can protect probiotics from being destroyed when passing through harsh environments such as stomach acid and bile, ensuring that probiotics can successfully reach the intestines and exert their effects.

[0077] Bifidobacteria can lower the pH value of the intestine by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, making it difficult for harmful bacteria to colonize in the intestine and thus reducing the risk of intestinal infection. Sodium alginate-pectin complex microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the structure of the intestinal flora, and improve the stability and health of the intestinal microecology.

[0078] Based on Example 1 and Comparative Example 2, and referring to Table 5, it can be seen that, compared with Example 1, the average daily weight gain of Comparative Example 2 is significantly reduced, while the diarrhea rate of Comparative Example 2 is significantly increased. This indicates that, compared with not adding vitamin E, adding vitamin E can effectively reduce the diarrhea rate of piglets, thereby improving the intestinal health of piglets.

[0079] The reason for this is that vitamin E can protect intestinal cells from free radical damage by neutralizing free radicals, maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa can act as a physical barrier, making it difficult for pathogens to invade, thereby reducing the risk of intestinal infection. Vitamin E can regulate the immune function of animals, enhance the local immune response in the intestine, and improve resistance to intestinal pathogens. At the same time, during processing and storage, vitamin E can reduce the damage of oxidative stress to probiotics by neutralizing free radicals and maintain their activity. Probiotics can activate gut-associated lymphoid tissue and promote the production of secretory immunoglobulin A, working together with vitamin E to build a systemic immune defense.

[0080] Based on Examples 1 and 2-3 and Table 5, it can be seen that, compared with Example 1, the average daily weight gain of Examples 2 and 3 was lower, and the diarrhea rate of Examples 2 and 3 was higher. This indicates that the amount of each component of the natural mixed feed additive affects the intestinal health of piglets.

[0081] Combining Examples 1 and 4 with Table 5, it can be seen that, compared with Example 1, the average daily weight gain in Example 4 decreased significantly, and the diarrhea rate in Example 4 also increased significantly. This indicates that, compared with adding conventional probiotics, adding modified probiotic microcapsules can effectively improve the intestinal health of piglets.

[0082] Combining Examples 1 and 5-6 with Table 5, it can be seen that, compared with Example 1, the average daily weight gain of Examples 5 and 6 is lower, and the diarrhea rate of Examples 5 and 6 is also significantly increased. This indicates that the amount of each component added to the modified probiotic microcapsule affects the intestinal health of piglets.

[0083] Based on Examples 1 and 7-8 and Table 5, it can be seen that, compared with Example 1, the average daily weight gain of Examples 7 and 8 is lower, and the diarrhea rate of Examples 7 and 8 is also significantly increased. This indicates that the amount of sodium alginate-pectin compound microencapsulated probiotics added affects the intestinal health of piglets.

[0084] Based on Examples 1 and 9-10 and Table 5, it can be seen that, compared with Example 1, the average daily weight gain of Examples 9 and 10 is lower, and the diarrhea rate of Examples 9 and 10 is also significantly increased. This indicates that the amount of sodium alginate-pectin compound solution added affects the intestinal health of piglets.

[0085] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A natural mixed feed additive, characterized in that, The modified probiotic microcapsule comprises the following raw materials in parts by mass: 1-2 parts of Bifidobacterium, and 3-5 parts of sodium alginate-pectin composite microencapsulated probiotics. The preparation method of the modified probiotic microcapsule comprises the following steps: placing 3-5 parts of sodium alginate-pectin composite microencapsulated probiotics in MRS culture medium for secondary fermentation to obtain the modified probiotic microcapsule. The preparation method of the sodium alginate-pectin composite microencapsulated probiotics comprises the following steps: weighing 0.3-0.7 g of calcium carbonate and dissolving it in 6-14 ml of deionized water, stirring it uniformly with 2-4 ml of a sodium alginate-pectin composite solution, then adding 1-3 ml of concentrated bacterial solution and mixing it uniformly, then dispersing it in 15-25 ml of liquid paraffin and stirring it at 350-450 r / min for 4-6 min, then adding 0.5-1.5 ml of glacial acetic acid, reacting for 8-12 min, and finally rinsing it with deionized water for 2-4 times to obtain the sodium alginate-pectin composite microencapsulated probiotics. The preparation method of the concentrated bacterial solution comprises the following steps: culturing 1-2 parts of Bifidobacterium in liquid MRS culture medium at 35-39 °C for 16-20 h for activation, centrifuging the activated bacterial solution at 3500-4500 r / min for 8-12 min, washing the bacterial bodies with physiological saline for 1-3 times, and resuspending the washed bacterial bodies in physiological saline to obtain the concentrated bacterial solution. The modified probiotic microcapsule comprises the following raw materials in parts by mass: 1-2 parts of Bifidobacterium, and 3-5 parts of sodium alginate-pectin composite microencapsulated probiotics. The sodium alginate-pectin composite microencapsulated probiotics comprise the following raw materials: 2-4 ml of a sodium alginate-pectin composite solution, 1-3 ml of concentrated bacterial solution, 0.3-0.7 g of calcium carbonate, 6-14 ml of deionized water, 15-25 ml of liquid paraffin, and 0.5-1.5 ml of glacial acetic acid. The sodium alginate-pectin composite solution comprises the following raw materials: 0.1-0.3 g of naringin, 26-34 ml of deionized water, 0.3-0.5 g of pectin, and 0.6-1 g of sodium alginate. The preparation method of the sodium alginate-pectin composite solution comprises the following steps: weighing 0.1-0.3 g of naringin and adding it to 18-22 ml of deionized water, magnetically stirring the mixture at 55-65 °C and at 350-450 r / min for 25-35 min, then adding 0.3-0.5 g of pectin and continuing to stir for 0.5-1.5 h to obtain a mixed solution, weighing 0.6-1 g of sodium alginate and adding it to 8-12 ml of deionized water, magnetically stirring the mixture at 20-30 °C and at 350-450 r / min for 0.5-1.5 h for dissolution, then transferring the dissolved sodium alginate solution to the mixed solution and continuing to stir at 350-450 r / min for 0.5-1.5 h, and then quickly cooling the mixture in a 15-25 °C water bath for 25-35 min and removing air bubbles in the solution by ultrasonic treatment.

2. The method of claim 1, wherein the natural mixed feed additive is prepared by the steps of: The method comprises the following steps: S1: 10-16 parts of soybean oil is heated to 35-45℃, then 4-7 parts of vitamin E and 5-15 parts of beta-carotene are added and stirred; S2: 1-5 parts of emulsifier is added into 20-35 parts of deionized water, heated to 45-55℃ and stirred, then slowly added into the mixture obtained in S1; S3: 5-8 parts of modified probiotic microcapsules are added and stirred at 100-200 r / min for 8-12 min.

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