Composition with physical fatigue relieving effect as well as preparation method and application thereof

Abstract

The invention relates to the technical field of foods and / or medicines, and provides a composition with a physical fatigue relieving effect as well as a preparation method and application of the composition. Fructus lycii; raspberry; chinese magnoliavine fruit; semen cassiae; emblic leafflower fruit; hovenia dulcis thunb; gardenia; pawpaw; ginseng and hairy antler. The composition provided by the invention has the function of relieving physical fatigue, is beneficial to improving the anti-fatigue ability of a human body, improving the sub-health state and preventing chronic diseases, and has a good market development prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of food and / or medicine, and particularly relates to a composition having the function of relieving physical fatigue, and a preparation method and application thereof. Background Art

[0002] Sub-health is a major problem threatening human health today, and its incidence rate shows an increasing trend year by year. At present, a large population in China is in the sub-health state. The acceleration of the work rhythm, the decrease in the number of physical exercises, and unhealthy living habits are the main reasons for falling into the "sub-health" state. Through relevant research, it is found that fatigue is one of the most common symptoms in sub-health, accounting for more than 40% of the sub-health population, and is also one of the reasons that most affect the quality of life of the sub-health population.

[0003] Physical fatigue, also known as exercise-induced fatigue, refers to the phenomenon of the decline of the body's exercise ability caused by the body's movement, that is, "the physiological process cannot continue, and its function cannot maintain a specific level or the predetermined exercise intensity". With the acceleration of the modern life rhythm and the increase in work pressure, exercise-induced fatigue is closely related to people's daily life. The long-term fatigue state can change the body's energy metabolism mode, increase the accumulation of harmful metabolites, cause premature aging of the body, and become a risk factor for cardiovascular and cerebrovascular diseases.

[0004] According to the investigation of the World Health Organization, about 35% or more of the people in the world are in a state of fatigue, especially among middle-aged men, the proportion of those in a state of fatigue is as high as 60%-75%. Medical experts point out that continuous excessive fatigue can lead to a series of adverse symptoms and produce a "fatigue syndrome". According to data statistics, at present, more than half of the people in China are in a state of chronic fatigue syndrome, accounting for about 70% of the urban population.

[0005] With the continuous improvement of living standards, people's health awareness and health preservation concepts are getting stronger and stronger. They have changed from seeing a doctor when they are sick in the past to preventing diseases when they are not sick, so as to achieve the purpose of obtaining a healthy body. The focus of modern medicine has also shifted to the intervention of the pre-disease state and the effective prevention of diseases. Traditional Chinese medicine has unique characteristics in adjusting the overall function state of the body. Health foods made from traditional Chinese medicines with the function of relieving physical fatigue have irreplaceable advantages. Natural Chinese herbal medicines with health care functions have mild medicinal properties, small toxic and side effects, many active ingredients, a wide active spectrum, are not easy to produce drug resistance, and are suitable for long-term use.

[0006] Therefore, it is of great significance to develop a Chinese medicine composition for relieving physical fatigue that is efficient, safe and suitable for long-term use. Summary of the Invention

[0007] The purpose of the present invention is to provide a composition having the function of relieving physical fatigue, and a preparation method and application thereof.

[0008] To achieve the above-mentioned invention object, the present invention provides the following technical solutions:

[0009] A composition with the function of relieving physical fatigue, which is composed of the following raw materials in parts by weight: dodder 0.8 - 2.0 parts; wolfberry fruit 0.8 - 2.0 parts; raspberry 0.2 - 1.0 parts; schizandra fruit 0.2 - 1.0 parts; cassia seed 0.3 - 0.9 parts; phyllanthus emblica 0.1 - 0.7 parts; oriental raisin tree seed 0.1 - 0.7 parts; gardenia fruit 0.05 - 0.35 parts; papaya 0.3 - 0.9 parts; ginseng 0.5 - 1.5 parts and pilose antler 0.5 - 1.5 parts.

[0010] Further, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: dodder 1.0 - 1.5 parts; wolfberry fruit 1.0 - 1.5 parts; raspberry 0.3 - 0.9 parts; schizandra fruit 0.3 - 0.9 parts; cassia seed 0.4 - 0.8 parts; phyllanthus emblica 0.2 - 0.6 parts; oriental raisin tree seed 0.2 - 0.6 parts; gardenia fruit 0.15 - 0.3 parts; papaya 0.4 - 0.8 parts; ginseng 0.8 - 1.2 parts and pilose antler 0.8 - 1.2 parts.

[0011] Further, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: dodder 2.0 parts; wolfberry fruit 1.5 parts; raspberry 1.0 parts; schizandra fruit 0.9 parts; cassia seed 0.9 parts; phyllanthus emblica 0.7 parts; oriental raisin tree seed 0.6 parts; gardenia fruit 0.35 parts; papaya 0.8 parts; ginseng 1.5 parts and pilose antler 1.2 parts.

[0012] Further, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: dodder 0.8 parts; wolfberry fruit 1.0 parts; raspberry 0.2 parts; schizandra fruit 0.3 parts; cassia seed 0.3 parts; phyllanthus emblica 0.1 parts; oriental raisin tree seed 0.2 parts; gardenia fruit 0.05 parts; papaya 0.4 parts; ginseng 0.5 parts and pilose antler 0.8 parts.

[0013] Further, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: dodder 1.3 parts; wolfberry fruit 1.3 parts; raspberry 0.6 parts; schizandra fruit 0.6 parts; cassia seed 0.6 parts; phyllanthus emblica 0.4 parts; oriental raisin tree seed 0.4 parts; gardenia fruit 0.2 parts; papaya 0.6 parts; ginseng 1.0 parts and pilose antler 1.0 parts.

[0014] Further, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: dodder 1.0 parts; wolfberry fruit 0.8 parts; raspberry 0.3 parts; schizandra fruit 0.2 parts; cassia seed 0.4 parts; phyllanthus emblica 0.2 parts; oriental raisin tree seed 0.1 parts; gardenia fruit 0.15 parts; papaya 0.3 parts; ginseng 0.8 parts and pilose antler 0.5 parts.

[0015] Furthermore, the composition with the function of relieving physical fatigue is composed of the following raw materials in parts by weight: Cuscuta chinensis 1.5 parts; Lycium barbarum 2.0 parts; Rubus idaeus 0.9 parts; Schisandra chinensis 1.0 parts; Cassia obtusifolia 0.8 parts; Phyllanthus emblica 0.6 parts; Hovenia dulcis 0.7 parts; Gardenia jasminoides 0.3 parts; Chaenomeles sinensis 0.9 parts; Panax ginseng 1.2 parts and Cervus elaphus pilose antler 1.5 parts.

[0016] The present invention also provides a preparation method of the above composition with the function of relieving physical fatigue, which includes the following steps:

[0017] Take the slices of Panax ginseng and Cervus elaphus pilose antler and crush them respectively to obtain ginseng powder and Cervus elaphus pilose antler powder;

[0018] Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia dulcis, Gardenia jasminoides and Chaenomeles sinensis, reflux and decoct twice with water, filter and combine the filtrates to obtain an extract;

[0019] Concentrate the extract under reduced pressure to a relative density of 1.02 - 1.12 to obtain an extract paste. After drying the extract paste, obtain a dry paste. After crushing the dry paste, obtain dry paste powder;

[0020] The obtained ginseng powder, Cervus elaphus pilose antler powder and the dry paste powder together constitute the composition.

[0021] Furthermore, each reflux decoction is for 1.0 - 2.0 hours, and 7 - 11 times the amount of water is added for each reflux decoction; the temperature for concentration under reduced pressure is 50 - 80°C, and the vacuum degree is 0.04 - 0.09 MPa; the temperature for drying the extract paste is 110 - 120°C, and the vacuum degree is 0.04 - 0.10 MPa.

[0022] The present invention also provides the application of the above composition with the function of relieving physical fatigue in the preparation of health foods, functional foods or drugs.

[0023] The present invention also provides a functional food, which includes the above composition with the function of relieving physical fatigue.

[0024] In addition, the present invention also provides a health food, which includes the above composition with the function of relieving physical fatigue.

[0025] Furthermore, the health food also includes the following raw and auxiliary materials in parts by weight: microcrystalline cellulose 0.25 - 1.0 parts and magnesium stearate 0.01 - 0.05 parts.

[0026] Furthermore, the dosage form of the health food is granule, tablet or capsule.

[0027] Furthermore, the preparation method of the capsule of the health food includes the following steps:

[0028] S1. Crush ginseng and pilose antler pieces separately to obtain ginseng powder and pilose antler powder, and irradiate them for sterilization;

[0029] S2. Take semen cuscutae, fructus lycii, fructus rubi, schisandra chinensis, semen cassiae, phyllanthus emblica, fruit of oriental raisin tree, gardenia jasminoides and fructus chaenomelis, reflux and decoct them with water twice, filter and combine the filtrates to obtain an extract;

[0030] S3. Concentrate the extract under reduced pressure to a relative density of 1.02 - 1.12 to obtain an extract paste. After drying the extract paste, obtain a dry paste. After crushing the dry paste, obtain dry paste powder;

[0031] S4. Take the irradiated ginseng powder and pilose antler powder, mix them with microcrystalline cellulose and dry paste powder, granulate to obtain granules;

[0032] S5. Take the obtained granules and mix them with magnesium stearate to obtain total mixed granules;

[0033] S6. Fill the obtained total mixed granules into capsules to obtain the product.

[0034] Further, in step S1, the irradiation sterilization uses 60 Co-γ ray irradiation sterilization with an irradiation dose of 4 kGy; in step S2, each reflux decoction lasts for 1.0 - 2.0 hours, and 7 - 11 times the amount of water is added for each reflux decoction; in step S3, the temperature for concentration under reduced pressure is 50 - 80 °C, and the vacuum degree is 0.04 - 0.09 MPa; in step S3, the drying temperature is 110 - 120 °C, the vacuum degree is 0.04 - 0.10 Mpa, and the moisture content of the obtained dry paste is ≤ 5.0%.

[0035] Based on the understanding of chronic fatigue syndrome in traditional Chinese medicine theory, for the population with long-term physical fatigue, this invention determines the treatment method of nourishing and filling the true essence and tonifying the five zang-organs, and formulates the composition formula of this invention. The whole formula consists of semen cuscutae, fructus lycii, phyllanthus emblica, fruit of oriental raisin tree, schisandra chinensis, fructus rubi, semen cassiae, gardenia jasminoides, ginseng, pilose antler and fructus chaenomelis. It focuses on nourishing and filling the true essence to supplement the congenital essence, and at the same time tonifies the spleen, lung, heart and liver, so as to relieve physical fatigue and improve the body's vitality.

[0036] King drug: Semen Cuscutae, pungent, sweet, flat. It belongs to the liver, kidney and spleen meridians. It has the effects of tonifying the liver and kidney, astringing sperm and reducing urination, and improving eyesight. "Shennong Ben Cao Jing" records that it "supplements deficiencies, strengthens qi and strength, makes people fat and healthy, and improves eyesight after long-term use." "Leigong's Treatise on Drug Preparation" records that it can "supplement the body's defensive qi and assist the tendons and vessels." It can be seen that semen cuscutae can relieve fatigue and strengthen qi and strength by tonifying the kidney and filling the essence. Modern pharmacological research has confirmed that semen cuscutae can inhibit the oxidative damage of brain tissue caused by high-intensity exhaustive exercise by increasing the activity of antioxidant enzymes in brain tissue and delay the occurrence of fatigue.

[0037] Principal drug: Wolfberry fruit, sweet, neutral. It acts on the liver and kidney meridians. It has the effects of nourishing the liver and kidney, and improving eyesight. "Properties and Applications of Medicinal Herbs" says: "It tonifies essence, remedies deficiencies, changes complexion, turns white, improves eyesight... and makes people live long." "General Treatise on the Materia Medica" records that "wolfberry fruit tonifies the kidney and benefits essence. When water is abundant, bones become strong, and all symptoms such as wasting-thirst, blurred vision, and pain in the waist and knees can be cured." "Correct Treatise on Materia Medica" records that "wolfberry, with a strong and pure taste, can nourish yin. Since there is yang in yin, it can also tonify qi. Therefore, it nourishes yin without causing yin deficiency, and helps yang to make yang flourish.... It replenishes essence, strengthens the marrow, strengthens bones and muscles, and is good at treating overstrain." Pharmacological studies have confirmed that the water extract of wolfberry fruit can achieve the anti-fatigue effect by regulating the oxidative stress pathway. In addition, it also has the effects of delaying aging and enhancing immunity.

[0038] Assistant drug: Phyllanthus emblica, sweet, sour, astringent, cool. It acts on the lung and stomach meridians. It has the effects of promoting digestion and strengthening the stomach, and promoting the production of body fluid. "Supplements to the Materia Medica" records that it "is mainly used for tonifying and strengthening strength." The fruit of Phyllanthus emblica is rich in phenolic compounds, flavonoids, polysaccharides, terpenoids, sterols, fatty acids, vitamins, proteins, amino acids, trace elements, anthraquinones, alkaloids and other components, and has the effects of anti-hypoxia, delaying aging and anti-fatigue. It can achieve the anti-hypoxia purpose by increasing the content of hemoglobin (Hb) in the blood and reducing the oxygen consumption of the body. It can regulate the proportion of fat energy supply, increase the glycogen reserve, save the consumption of glycogen, and remove fatigue-causing substances, etc., to achieve anti-fatigue in many aspects.

[0039] Assistant drug: Semen Hoveniae, sweet, neutral. It acts on the heart, spleen and lung meridians. It has the effects of relieving alcohol toxicity, quenching thirst and dispelling vexation. "Illustrated Explanation of the Materia Medica of Yunnan" records that it has the effect of "tonifying the middle and replenishing qi". Semen Hoveniae is rich in carbohydrates, amino acids, flavonoid compounds, fatty acids, alkaloids and other components. Research shows that the ethanol extract of Semen Hoveniae can increase the glycogen reserve in the body and effectively reduce the accumulation of urea nitrogen in the body after exercise, and has a good anti-fatigue effect.

[0040] Assistant drug: Schisandra chinensis, sour, sweet, warm. It acts on the lung, heart and kidney meridians. It has the effects of astringing and consolidating, promoting the production of body fluid with qi-tonifying function, and tonifying the kidney and calming the heart. "Shennong's Classic of Materia Medica" records that it "is mainly used for tonifying qi, treating cough and dyspnea, treating overstrain and emaciation, remedying deficiencies, strengthening yin, and benefiting the essence of men." "Supplementary Records of Famous Physicians" records that it can "nourish the five zang-organs, remove heat, and generate muscle in the yin aspect." "General Treatise on the Materia Medica" records that it can "strengthen essence and astringe sweat." Pharmacological studies have confirmed that Schisandra chinensis can regulate immunity, blood sugar and blood lipids while relieving physical fatigue, and at the same time has a protective effect on the cardiovascular system.

[0041] Assistant drug: Fructus Rubi, sweet, sour, warm. It acts on the liver, kidney and bladder meridians. It has the effects of tonifying the kidney and consolidating essence, nourishing the liver and improving eyesight. "Supplementary Records of Famous Physicians" states that it "benefits qi and makes the body light, and prevents hair from turning white." "Treatise on Medicinal Properties" records that "in cases of kidney essence depletion in men, women who consume it can conceive." "Japanese Pharmacopoeia" records that it can "pacify the five viscera, improve complexion, nourish essence and qi, grow hair long, and strengthen the will." "Explanation of the Classic of Materia Medica" records that "for Fructus Rubi, when it is said to benefit qi, it means to benefit essence and qi. The kidney stores essence and receives qi. When essence and qi are sufficient, the body will be light and the hair will not turn white." It can be seen that Fructus Rubi is a drug for nourishing and filling true essence. When used together with Schisandra chinensis, it can achieve the effect of tonifying the kidney and consolidating essence. Modern research has confirmed that Fructus Rubi has antioxidant and anti-aging effects, and can also improve memory.

[0042] Assistant drug: Semen Cassiae, sweet, bitter, salty, slightly cold. It acts on the liver and large intestine meridians. It has the effects of clearing heat and improving eyesight, and moistening the intestines to relieve constipation. "Shennong Ben Cao Jing" records that it "treats blurred vision, excessive eye secretions, red and white membranes in the eyes, eye pain, lacrimation. Long-term use improves clear vision." "Treatise on Medicinal Properties" records that it "benefits the five viscera and clears heat in the liver." "Compendium of Materia Medica Justice" records that "Semen Cassiae improves eyesight by nourishing the liver and kidney, with the meaning of suppressing and supplementing yin. This is the correct treatment for strengthening the root, not comparable to the treatment of only symptomatic diseases by warming, pungent and dispersing wind, or cold and cooling heat. It is the most beneficial without harm." In this formula, it mainly plays the role of nourishing the liver and kidney and benefiting the five viscera. Modern research has confirmed that Semen Cassiae has the effects of enhancing memory and anti-aging.

[0043] Assistant drug: Fructus Gardeniae, bitter, cold. It acts on the heart, lung and triple energizer meridians. It has the effects of purging fire to remove vexation, clearing heat and promoting diuresis, and cooling blood to detoxify. "Shennong Ben Cao Jing" says that it "treats pathogenic factors in the five internal organs, heat in the stomach, facial flushing, rosacea, leukoderma, erythroderma, sores and ulcers." "Supplementary Records of Famous Physicians" records that it can "treat eye heat and pain, great heat in the chest, heart, large and small intestines, vexation and restlessness in the heart, and heat in the stomach." "Medical Origins" records that it "treats pathogenic heat in the heart meridian, removes restlessness, clears deficiency heat in the upper jiao, and treats wind." Zhu Zhenheng believed that Fructus Gardeniae can "purging fire in the triple energizer, clearing blood in the stomach cavity, treating heat syncope with heart pain, relieving heat stagnation, and promoting qi circulation." It can be seen that Fructus Gardeniae has the effect of clearing heat in the heart and stomach. When applied here, it has the effect of preventing stagnation during supplementation. Modern research has confirmed that Fructus Gardeniae has anti-fatigue and antioxidant effects.

[0044] Assistant drug: Ginseng, sweet, slightly bitter, slightly warm. It acts on the spleen, lung, heart and kidney meridians. It has the effects of greatly tonifying primordial qi, restoring pulse and arresting collapse, invigorating the spleen and benefiting the lung, promoting fluid production and nourishing blood, and tranquilizing the mind and improving intelligence. "Shennong Ben Cao Jing" says that it "mainly supplements the five viscera, tranquilizes the mind, stops palpitation, eliminates pathogenic factors, improves eyesight, and makes the mind happy and intelligent." "Treatise on Medicinal Properties" records that it "treats insufficiency of qi in the five viscera, five kinds of overstrain and seven kinds of injuries, deficiency and debility, emesis and counterflow, inability to eat, stops cholera, vexation, vomiting and retching, supplements the five viscera and six fu-organs, and protects the middle and guards the spirit." Modern research has confirmed that ginseng can increase the content of liver glycogen and various antioxidant enzymes, reduce urea accumulation to play an anti-fatigue role, and also has anti-aging and immune regulation effects.

[0045] Assistant herbs: Pilose antler of red deer, sweet, salty, warm. It acts on the kidney and liver meridians. It has the effects of strengthening yang in the kidney, benefiting essence and blood, strengthening muscles and bones, regulating thoroughfare and conception vessels, and expelling toxin of sores. Recorded in "On Medicinal Properties": "It is mainly used to tonify the deficiency and cold in the loins and knees of men, weakness of the feet and knees, dreamful intercourse, involuntary emission of essence, metrorrhagia and metrostaxis in women." Recorded in "Japanese Pharmacopoeia of Chinese Materia Medica": "It can tonify deficiency and weakness, strengthen muscles and bones, break stasis of blood, and arrest threatened abortion and relieve qi stagnation." Recorded in "Compendium of Materia Medica": "It can generate essence, nourish marrow, nourish blood and benefit yang, strengthen muscles and bones. It can treat all kinds of consumptive diseases, deafness, dim eyesight, dizziness, and deficiency dysentery." Pilose antler of red deer is a kind of medicine with flesh and blood, which can tonify the kidney and replenish essence. When combined with the son herbs, they complement each other. Modern research has confirmed that pilose antler of red deer has the effects of anti-fatigue, improving learning and memory ability, anti-aging, and antioxidant.

[0046] Guiding herb: Chinese flowering quince, sour, warm. It acts on the liver and spleen meridians. It has the effects of relaxing tendons and activating collaterals, and regulating the stomach and promoting digestion. Recorded in "Explanation of Medical Properties": "Chinese flowering quince, sour in taste, cold in nature, non-toxic, acts on the lung, spleen and liver meridians. It mainly treats beriberi edema, abdominal cold and heat pain, and rushing syncope, expels dampness, regulates nutrient qi and defensive qi, promotes the function of the spleen and stomach, and stops vomiting and diarrhea." Modern research has confirmed that Chinese flowering quince has antioxidant effect.

[0047] Eight kinds of seed drugs are selected in the present invention. In traditional Chinese medicine, there is a way of thinking in using drugs that "like qi attracts like qi", using seeds to tonify the body and nourish essence. In the monograph on materia medica in the Ming Dynasty, "Just Righteousness of Materia Medica", when discussing plant seed drugs, it is said that "all seeds are firm, tonifying the yin of the five zang-organs and benefiting essence and qi, all seeds are heavy, tonifying the kidney and strengthening kidney qi". Among the eight seeds, dodder seeds and wolfberries nourish the kidney essence and are used together as the monarch herbs. Phyllanthus emblica and oriental raisin tree seeds tonify the middle-jiao and replenish qi, nourishing the five zang-organs, and schizandra berries and raspberry fruits tonify the kidney and consolidate essence, and the four are the minister herbs. The medicinal flavors of the composition of the present invention are mainly tonifying agents, assisted by cassia seeds to nourish the liver and kidney and benefit the five zang-organs, and gardenia fruits to purge fire and relieve vexation. The combination of the two has the effect of tonifying without stagnation. On the basis of the above-mentioned use of drugs, ginseng is combined to tonify the five zang-organs and assist the kidney qi; pilose antler of red deer, a medicine with flesh and blood, tonifies the kidney and replenishes essence, and when combined with the son herbs, they complement each other, and both are used as assistant herbs. Chinese flowering quince is used as the guiding herb, relaxing tendons and activating collaterals, regulating the stomach and promoting digestion to relieve symptoms such as heaviness of limbs and loss of appetite caused by physical fatigue. All the herbs are combined to nourish the true essence, tonify the five zang-organs, relieve physical fatigue fundamentally, and enhance the internal power of the body.

[0048] Compared with the prior art, the composition with the effect of relieving physical fatigue provided by the present invention is composed of the dry extract powder of the water extract of Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia dulcis, Gardenia jasminoides and Chaenomeles sinensis, and ginseng powder and pilose antler powder in a specific ratio. After the combination of each component, it has a synergistic anti-fatigue effect, and the obtained composition has a good function of relieving physical fatigue. This composition can significantly prolong the weight-bearing swimming time of mice, can significantly increase the glycogen reserve in the liver of mice, can significantly reduce the serum urea level after exercise of mice, can effectively enhance the exercise endurance of mice, reduce the accumulation of the metabolite urea in the serum, increase the glycogen content in the liver of mice, increase the energy reserve of the body, and thus delay the appearance of fatigue. The composition provided by the present invention can be used as an active ingredient to prepare foods, health foods or drugs with the effect of relieving physical fatigue, meeting the new demands of people for health, helping to improve the anti-fatigue ability of the human body, improving the sub-healthy state and preventing chronic diseases, and having good market development prospects. Detailed Embodiments

[0049] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0050] Example 1

[0051] A composition with the effect of relieving physical fatigue is made from the following raw materials in parts by weight: 2.0 parts of Cuscuta chinensis; 1.5 parts of Lycium barbarum; 1.0 part of Rubus idaeus; 0.9 part of Schisandra chinensis; 0.9 part of Cassia obtusifolia; 0.7 part of Phyllanthus emblica; 0.6 part of Hovenia dulcis; 0.35 part of Gardenia jasminoides; 0.8 part of Chaenomeles sinensis; 1.5 parts of ginseng and 1.2 parts of pilose antler.

[0052] The preparation method of the composition with the effect of relieving physical fatigue is as follows:

[0053] (1) Take the ginseng and pilose antler slices and crush them respectively to obtain ginseng powder and pilose antler powder (irradiation sterilization);

[0054] (2) Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia dulcis, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice, each time for 1.5 hours, add 10 times of water each time, filter and combine the filtrates to obtain an extract;

[0055] (3) Concentrate the extract under reduced pressure (concentration temperature: 60 °C, vacuum degree: 0.06 MPa) to a relative density of 1.02 - 1.12 (60 °C) to obtain an extract paste. After the extract paste is dried at a drying temperature of 118 °C and a vacuum degree of 0.05 Mpa, a dry extract paste (water content ≤ 5.0%) is obtained. The dry extract paste is crushed (sieve mesh size: 80 meshes), and the dry extract powder is collected;

[0056] The obtained ginseng powder, pilose antler powder and the obtained dry extract powder together constitute the above composition.

[0057] Example 2

[0058] A composition with the function of relieving physical fatigue, comprising the following raw materials in parts by weight: dodder seed 0.8 part; wolfberry fruit 1.0 part; raspberry 0.2 part; schizandra fruit 0.3 part; cassia seed 0.3 part; phyllanthus emblica 0.1 part;hovenia dulcis 0.2 part; gardenia fruit 0.05 part; papaya 0.4 part; ginseng 0.5 part and pilose antler 0.8 part.

[0059] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0060] (1) Take the ginseng and pilose antler pieces and crush them separately to obtain ginseng powder and pilose antler powder (irradiation sterilization);

[0061] (2) Take dodder seed, wolfberry fruit, raspberry, schizandra fruit, cassia seed, phyllanthus emblica, hovenia dulcis, gardenia fruit and papaya according to the formula ratio, reflux and decoct with water twice, each time for 1.0 hour, add 11 times of water each time, filter and combine the filtrates to obtain an extract;

[0062] (3) Concentrate the extract under reduced pressure (concentration temperature is 80 °C, vacuum degree is 0.04 MPa) to a relative density of 1.02 - 1.12 (60 °C) to obtain an extract paste. After drying the extract paste at a drying temperature of 120 °C and a vacuum degree of 0.05 Mpa, obtain a dry extract (water content ≤ 5.0%), crush the dry extract (mesh number of sieve: 80 meshes), and collect the dry extract powder;

[0063] The obtained ginseng powder, pilose antler powder and the obtained dry extract powder together constitute the above composition.

[0064] Example 3

[0065] A composition with the function of relieving physical fatigue, comprising the following raw materials in parts by weight: dodder seed 1.0 part; wolfberry fruit 0.8 part; raspberry 0.3 part; schizandra fruit 0.2 part; cassia seed 0.4 part; phyllanthus emblica 0.2 part; hovenia dulcis 0.1 part; gardenia fruit 0.15 part; papaya 0.3 part; ginseng 0.8 part and pilose antler 0.5 part.

[0066] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0067] (1) Take the ginseng and pilose antler pieces and crush them separately to obtain ginseng powder and pilose antler powder (irradiation sterilization);

[0068] (2) Take dodder seeds, wolfberries, raspberry fruits, schisandra fruits, cassia seeds, phyllanthus emblica fruits, oriental raisin tree fruits, gardenia fruits and papaya according to the formula ratio, reflux and decoct with water twice, each time for 2.0 hours, add 7 times the amount of water each time, filter and combine the filtrates to obtain an extract;

[0069] (3) Concentrate the extract under reduced pressure (concentration temperature is 50 °C, vacuum degree is 0.09 MPa) to a relative density of 1.02 - 1.12 (60 °C) to obtain an extract paste. After drying the extract paste under the conditions of a drying temperature of 110 °C and a vacuum degree of 0.10 Mpa, obtain a dry paste (water content ≤ 5.0%). Crush the dry paste (sieve mesh size: 80 mesh) and collect the dry paste powder;

[0070] The obtained ginseng powder, pilose antler powder and the obtained dry paste powder together constitute the above-mentioned composition.

[0071] Example 4

[0072] A composition with the function of relieving physical fatigue, comprising the following raw materials in parts by weight: 1.5 parts of dodder seeds; 2.0 parts of wolfberries; 0.9 parts of raspberry fruits; 1.0 part of schisandra fruits; 0.8 part of cassia seeds; 0.6 part of phyllanthus emblica fruits; 0.7 part of oriental raisin tree fruits; 0.3 part of gardenia fruits; 0.9 part of papaya; 1.2 parts of ginseng and 1.5 parts of pilose antler.

[0073] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0074] (1) Take ginseng and pilose antler slices and crush them respectively to obtain ginseng powder and pilose antler powder (irradiated and sterilized);

[0075] (2) Weigh dodder seeds, wolfberries, raspberry fruits, schisandra fruits, cassia seeds, phyllanthus emblica fruits, oriental raisin tree fruits, gardenia fruits and papaya according to the formula ratio, reflux and decoct with water twice, each time for 1.5 hours, add 10 times the amount of water each time, filter and combine the filtrates to obtain an extract;

[0076] (3) Concentrate the extract under reduced pressure (concentration temperature is 70 °C, vacuum degree is 0.08 MPa) to a relative density of 1.02 - 1.12 (60 °C) to obtain an extract paste. After drying the extract paste under the conditions of a drying temperature of 115 °C and a vacuum degree of 0.06 Mpa, obtain a dry paste (water content ≤ 5.0%). Crush the dry paste (sieve mesh size: 80 mesh) and collect the dry paste powder;

[0077] The obtained ginseng powder, pilose antler powder and the obtained dry paste powder together constitute the above-mentioned composition.

[0078] Example 5

[0079] A composition with the function of relieving physical fatigue, comprising the following raw materials in parts by weight: 1.3 parts of Cuscuta chinensis; 1.3 parts of Lycium barbarum; 0.6 part of Rubus idaeus; 0.6 part of Schisandra chinensis; 0.6 part of Cassia obtusifolia; 0.4 part of Phyllanthus emblica; 0.4 part of Hovenia acerba; 0.2 part of Gardenia jasminoides; 0.6 part of Chaenomeles sinensis; 1.0 part of Panax ginseng and 1.0 part of Cornu Cervi Pantotrichum.

[0080] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0081] (1) Take the slices of Panax ginseng and Cornu Cervi Pantotrichum and pulverize them respectively to obtain ginseng powder and Cornu Cervi Pantotrichum powder (irradiation sterilization);

[0082] (2) Weigh Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia acerba, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice. The first time is 1.5 hours and 10 times the amount of water is added for the first time. The second time is 1.5 hours and 8 times the amount of water is added for the second time. Filter and combine the filtrates to obtain an extract;

[0083] (3) Concentrate the extract under reduced pressure (concentration temperature is 65 °C, vacuum degree is 0.07 MPa) to a relative density of 1.02 - 1.12 (60 °C) to obtain an extract paste. After drying the extract paste under the conditions of a drying temperature of 115 °C and a vacuum degree of 0.08 Mpa, a dry paste is obtained (moisture content ≤ 5.0%). Pulverize the dry paste (sieve mesh size: 80 mesh) and collect the dry paste powder;

[0084] The obtained ginseng powder, Cornu Cervi Pantotrichum powder and the obtained dry paste powder together constitute the above composition.

[0085] Example 6

[0086] A health food, comprising the composition with the function of relieving physical fatigue obtained in Example 1. The raw and auxiliary materials are as follows:

[0087] 2.0 parts of Cuscuta chinensis; 1.5 parts of Lycium barbarum; 1.0 part of Rubus idaeus; 0.9 part of Schisandra chinensis; 0.9 part of Cassia obtusifolia; 0.7 part of Phyllanthus emblica; 0.6 part of Hovenia acerba; 0.35 part of Gardenia jasminoides; 0.8 part of Chaenomeles sinensis; 1.5 parts of Panax ginseng; 1.2 parts of Cornu Cervi Pantotrichum; 1.0 part of microcrystalline cellulose and 0.05 part of magnesium stearate.

[0088] The preparation method of the above health food capsules is as follows:

[0089] S1. Take the slices of Panax ginseng and Cornu Cervi Pantotrichum and pulverize them respectively to obtain ginseng powder and Cornu Cervi Pantotrichum powder, and irradiate for sterilization;

[0090] S2. Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia acerba, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice, each time for 1.5 hours and 10 times the amount of water is added each time. Filter and combine the filtrates to obtain an extract;

[0091] S3. Concentrate the extract under reduced pressure (concentration temperature: 60°C, vacuum degree: 0.06 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste at a drying temperature of 118°C and a vacuum degree of 0.05 Mpa, obtain a dry paste (moisture content ≤ 5.0%). Crush the dry paste (mesh number of sieve: 80 meshes), and collect the dry paste powder;

[0092] S4. Take the irradiated ginseng powder and pilose antler powder, mix them with microcrystalline cellulose and dry paste powder, and granulate to obtain granules;

[0093] S5. Mix the obtained granules with magnesium stearate to obtain the total mixed granules;

[0094] S6. Fill the obtained total mixed granules into capsules to obtain the product.

[0095] Example 7

[0096] A health food, comprising the composition with the function of relieving physical fatigue obtained in Example 3. The raw and auxiliary materials are as follows:

[0097] Cuscuta chinensis Lam. 1.0 part; Lycium barbarum L. 0.8 part; Rubus chingii Hu 0.3 part; Schisandra chinensis (Turcz.) Baill. 0.2 part; Cassia obtusifolia L. 0.4 part; Phyllanthus emblica L. 0.2 part; Hovenia acerba Lindl. 0.1 part; Gardenia jasminoides Ellis 0.15 part; Chaenomeles sinensis (Thouin) Koehne 0.3 part; Ginseng 0.8 part; Pilose antler 0.5 part; Microcrystalline cellulose 0.25 part; and magnesium stearate 0.01 part.

[0098] The preparation method of the above health food capsules is as follows:

[0099] S1. Take the ginseng and pilose antler slices, crush them respectively to obtain ginseng powder and pilose antler powder, and irradiate for sterilization;

[0100] S2. Take Cuscuta chinensis Lam., Lycium barbarum L., Rubus chingii Hu, Schisandra chinensis (Turcz.) Baill., Cassia obtusifolia L., Phyllanthus emblica L., Hovenia acerba Lindl., Gardenia jasminoides Ellis and Chaenomeles sinensis (Thouin) Koehne according to the formula ratio, reflux and decoct with water twice, 2.0 hours each time, add 7 times of water each time, filter and combine the filtrates to obtain an extract;

[0101] S3. Concentrate the extract under reduced pressure (concentration temperature: 50°C, vacuum degree: 0.09 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste at a drying temperature of 110°C and a vacuum degree of 0.10 Mpa, obtain a dry paste (moisture content ≤ 5.0%). Crush the dry paste (mesh number of sieve: 80 meshes), and collect the dry paste powder;

[0102] S4. Take the irradiated ginseng powder and pilose antler powder, mix them with microcrystalline cellulose and dry paste powder, and granulate to obtain granules;

[0103] S5. Mix the obtained granules with magnesium stearate to obtain the total mixed granules;

[0104] S6. Take the obtained total mixed granules to fill capsules, and thus obtain the product.

[0105] Example 8

[0106] A health food, comprising the composition with the function of relieving physical fatigue obtained in Example 5. The raw and auxiliary materials are as follows:

[0107] 1.3 parts of Cuscuta chinensis; 1.3 parts of Lycium barbarum; 0.6 part of Rubus idaeus; 0.6 part of Schisandra chinensis; 0.6 part of Cassia obtusifolia; 0.4 part of Phyllanthus emblica; 0.4 part of Hovenia dulcis; 0.2 part of Gardenia jasminoides; 0.6 part of Chaenomeles sinensis; 1.0 part of Panax ginseng; 1.0 part of Cervus nippon; 0.484 part of microcrystalline cellulose and 0.02 part of magnesium stearate.

[0108] The preparation method of the above health food capsules is as follows:

[0109] S1. Take the slices of Panax ginseng and Cervus nippon, crush them respectively to obtain ginseng powder and Cervus nippon powder, and irradiate and sterilize them;

[0110] S2. Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia dulcis, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice, 1.5 hours for the first time, add 10 times of water for the first time, 1.5 hours for the second time, add 8 times of water for the second time, filter and combine the filtrates to obtain an extract;

[0111] S3. Concentrate the extract under reduced pressure (concentration temperature: 65°C, vacuum degree: 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste at a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, obtain a dry paste (moisture content ≤ 5.0%), crush the dry paste (sieve mesh size: 80 meshes), and collect the dry paste powder;

[0112] S4. Take the irradiated ginseng powder and Cervus nippon powder, mix them with microcrystalline cellulose and dry paste powder, granulate to obtain granules;

[0113] S5. Take the obtained granules and mix them with magnesium stearate to obtain total mixed granules;

[0114] S6. Take the obtained total mixed granules to fill capsules, and thus obtain the product.

[0115] In order to explore and verify the influence of the ratio and dosage of each component of the composition on the function of relieving physical fatigue of the composition, the present invention is illustrated by the following comparative examples through experiments.

[0116] Comparative Example 1

[0117] A composition is made from the following raw materials in parts by weight: 2.5 parts of Cuscuta chinensis; 2.5 parts of Lycium barbarum; 1.2 parts of Rubus idaeus; 1.2 parts of Schisandra chinensis; 1.2 parts of Cassia obtusifolia; 0.8 part of Phyllanthus emblica; 0.8 part of Hovenia acerba; 0.4 part of Gardenia jasminoides; 1.2 parts of Chaenomeles sinensis; 2.0 parts of Panax ginseng and 1.0 part of Cornu Cervi Pantotrichum.

[0118] The preparation method of this composition is as follows:

[0119] (1) Take the slices of Panax ginseng and Cornu Cervi Pantotrichum and crush them separately to obtain ginseng powder and Cornu Cervi Pantotrichum powder (irradiation sterilization);

[0120] (2) Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia acerba, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice. The first time is 1.5 hours with 10 times the amount of water added, and the second time is 1.5 hours with 8 times the amount of water added. Filter and combine the filtrates to obtain an extract;

[0121] (3) Concentrate the extract under reduced pressure (concentration temperature is 65°C, vacuum degree is 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste at a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, obtain a dry paste (moisture content ≤ 5.0%). Crush the dry paste (sieve mesh size: 80 meshes) and collect the dry paste powder;

[0122] The obtained ginseng powder, Cornu Cervi Pantotrichum powder and the obtained dry paste powder together constitute the above composition.

[0123] Comparative Example 2

[0124] A composition is made from the following raw materials in parts by weight: 3.8 parts of Cuscuta chinensis; 3.8 parts of Lycium barbarum; 1.8 parts of Rubus idaeus; 1.8 parts of Schisandra chinensis; 1.8 parts of Cassia obtusifolia; 1.2 parts of Phyllanthus emblica; 1.2 parts of Hovenia acerba; 0.6 part of Gardenia jasminoides; 1.8 parts of Chaenomeles sinensis; 1.0 part of Panax ginseng and 0.5 part of Cornu Cervi Pantotrichum.

[0125] The preparation method of this composition is as follows:

[0126] (1) Take the slices of Panax ginseng and Cornu Cervi Pantotrichum and crush them separately to obtain ginseng powder and Cornu Cervi Pantotrichum powder (irradiation sterilization);

[0127] (2) Take Cuscuta chinensis, Lycium barbarum, Rubus idaeus, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia acerba, Gardenia jasminoides and Chaenomeles sinensis according to the formula ratio, reflux and decoct with water twice. The first time is 1.5 hours with 10 times the amount of water added, and the second time is 1.5 hours with 8 times the amount of water added. Filter and combine the filtrates to obtain an extract;

[0128] (3) The extract is concentrated under reduced pressure (concentration temperature: 65°C, vacuum degree: 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After the extract paste is dried under the conditions of a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, a dry paste (water content ≤ 5.0%) is obtained. The dry paste is pulverized (mesh number of the sieve: 80 meshes), and the dry paste powder is collected;

[0129] The obtained ginseng powder, pilose antler powder and the obtained dry paste powder together constitute the above-mentioned composition.

[0130] Comparative Example 3

[0131] A composition is prepared from the following raw materials in parts by weight: dodder seed 1.3 parts; wolfberry fruit 1.3 parts; raspberry 0.6 parts; schisandra fruit 0.6 parts; cassia seed 0.6 parts; phyllanthus emblica 0.4 parts;hovenia dulcis 0.4 parts; gardenia fruit 0.2 parts; papaya 0.6 parts; ginseng 2.0 parts and pilose antler 1.0 part.

[0132] The preparation method of the composition is as follows:

[0133] (1) Take the ginseng and pilose antler slices and pulverize them separately to obtain ginseng powder and pilose antler powder (irradiation sterilization);

[0134] (2) Take dodder seed, wolfberry fruit, raspberry, schisandra fruit, cassia seed, phyllanthus emblica, hovenia dulcis, gardenia fruit and papaya according to the formula ratio, and decoct them with water by refluxing twice. The first time is for 1.5 hours with 10 times the amount of water added, and the second time is for 1.5 hours with 8 times the amount of water added. Filter and combine the filtrates to obtain an extract;

[0135] (3) The extract is concentrated under reduced pressure (concentration temperature: 65°C, vacuum degree: 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After the extract paste is dried under the conditions of a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, a dry paste (water content ≤ 5.0%) is obtained. The dry paste is pulverized (mesh number of the sieve: 80 meshes), and the dry paste powder is collected;

[0136] The obtained ginseng powder, pilose antler powder and the obtained dry paste powder together constitute the above-mentioned composition.

[0137] Comparative Example 4

[0138] A composition includes the following raw materials in parts by weight: dodder seed 1.3 parts; wolfberry fruit 1.3 parts; raspberry 0.6 parts; mulberry fruit 0.6 parts; cassia seed 0.6 parts; phyllanthus emblica 0.4 parts; hovenia dulcis 0.4 parts; gardenia fruit 0.2 parts; papaya 0.6 parts; ginseng 1.0 part and epimedium 1.0 part.

[0139] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0140] (1) Crush the ginseng and epimedium slices separately to obtain ginseng powder and pilose antler powder (irradiated and sterilized);

[0141] (2) Weigh dodder seeds, wolfberries, raspberry fruits, mulberries, cassia seeds, phyllanthus emblica fruits, oriental raisin tree seeds, gardenia fruits and Chinese flowering quince according to the formula ratio, and reflux and decoct them with water twice. The first time is for 1.5 hours with 10 times the amount of water added, and the second time is for 1.5 hours with 8 times the amount of water added. Filter and combine the filtrates to obtain an extract;

[0142] (3) Concentrate the extract under reduced pressure (concentration temperature is 65°C, vacuum degree is 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste under the conditions of a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, obtain a dry paste (moisture content ≤ 5.0%). Crush the dry paste (sieve mesh size: 80 mesh) and collect the dry paste powder;

[0143] The obtained ginseng powder, epimedium powder and the obtained dry paste powder together constitute the above - mentioned composition.

[0144] Comparative Example 5

[0145] A composition, comprising the following raw materials in parts by weight: dodder seeds 1.3 parts; wolfberries 1.3 parts; raspberry fruits 0.6 parts; schisandra fruits 0.6 parts and plantain seeds 0.6 parts.

[0146] The preparation method of the composition with the function of relieving physical fatigue is as follows:

[0147] (1) Weigh dodder seeds, wolfberries, raspberry fruits, schisandra fruits and plantain seeds according to the formula ratio, and reflux and decoct them with water twice. The first time is for 1.5 hours with 10 times the amount of water added, and the second time is for 1.5 hours with 8 times the amount of water added. Filter and combine the filtrates to obtain an extract;

[0148] (2) Concentrate the extract under reduced pressure (concentration temperature is 65°C, vacuum degree is 0.07 MPa) to a relative density of 1.02 - 1.12 (60°C) to obtain an extract paste. After drying the extract paste under the conditions of a drying temperature of 115°C and a vacuum degree of 0.08 Mpa, obtain a dry paste (moisture content ≤ 5.0%). Crush the dry paste (sieve mesh size: 80 mesh) and collect the dry paste powder. The obtained dry paste powder together constitutes the above - mentioned composition.

[0149] Test Example 1

[0150] In order to better verify the characteristics of the composition of the present invention, an animal experiment study on the function of relieving physical fatigue was conducted on the composition of Example 5 and the compositions of Comparative Examples 1 - 3.

[0151] 1 Materials and Methods

[0152] 1.1 Samples:

[0153] The recommended oral doses for the samples are as follows: 3.50 g per day for Example 5, 5.95 g per day for Comparative Example 1, 5.95 g per day for Comparative Example 2, and 4.50 g per day for Comparative Example 3. Calculated based on an adult body weight of 60 kg, the converted doses are: 0.0583 g / kg·bw for Example 5, 0.0992 g / kg·bw for Comparative Example 1, 0.0992 g / kg·bw for Comparative Example 2, and 0.075 g / kg·bw for Comparative Example 3.

[0154] 1.2 Experimental animals:

[0155] 520 SPF-grade ICR male mice weighing 18 g - 22 g (Changsha Tianqin Biotechnology Co., Ltd.). The production license number for experimental animals is SCXK(Xiang)2019-0013. The animals were raised in a barrier environment at a temperature of 21°C - 24°C and a humidity of 50% - 58%. The license number for the use of experimental animals is SYXK(Xiang)2020-0011.

[0156] 1.3 Main instruments and reagents:

[0157] Automatic biochemical analyzer (BECKMAN COULTER AU680), blood lactate analyzer (EKF Lactatescout), 722S spectrophotometer, small animal swimming tank (approx. 53 cm × 40 cm × 35 cm), centrifuge, cell disruptor, water bath, shaker, electronic balance, etc. Serum urea test kit (Shanghai Fosun Changzheng Medical Science Co., Ltd.), liver glycogen test kit (Nanjing Jiancheng Bioengineering Institute).

[0158] 1.4 Experimental methods

[0159] The mice were divided into 4 major groups, namely Group I, Group II, Group III, and Group IV, with 130 mice in each major group. The weight-bearing swimming experiment, serum urea, blood lactic acid, and liver glycogen determination were carried out respectively. Each major group was randomly divided into 13 small groups, namely: blank control group, low-dose, medium-dose, and high-dose groups of Example 5 and Comparative Examples 1-3, with 10 mice in each group. The low-dose, medium-dose, and high-dose of Example 5 and Comparative Examples 1-3 were set according to 5, 10, and 30 times of the recommended oral dose for humans. Example 5 (0.292 g / kg·bw, 0.583 g / kg·bw, 1.750 g / kg·bw); Comparative Example 1 (0.496 g / kg·bw, 0.992 g / kg·bw, 2.975 g / kg·bw); Comparative Example 2 (0.496 g / kg·bw, 0.992 g / kg·bw, 2.975 g / kg·bw); Comparative Example 3 (0.375 g / kg·bw, 0.750 g / kg·bw, 2.250 g / kg·bw). At the same time, a blank control group was established. Each group was given intragastric administration to the test animals, and the intragastric administration volume was 0.2 ml / 10 g·bw for 30 consecutive days. Each index of the anti-fatigue test was carried out with reference to the standard "Methods for Function Testing and Evaluation of Health Foods (2023 Edition)".

[0160] 1.5 Statistical analysis

[0161] Statistical analysis was performed using Excel 2007 and Spss 19.0 software. The variances of the data in each group were homogeneous, and one-way analysis of variance and Dunnett's method were used for statistical analysis; if the variances were inhomogeneous, the variables were transformed or rank sum test and Tamhane's T2 test were used for statistical analysis.

[0162] 2 Results

[0163] 2.1 Effects on mouse body weight

[0164] Compared with the control group, there were no statistically significant differences in the initial body weight, weekly body weight, and final body weight of the mice in each dose group (P>0.05), as shown in Tables 1-4.

[0165] Table 1 Effects on mouse body weight (Group I - weight-bearing swimming)

[0166]

[0167]

[0168] Table 2 Effects on mouse body weight (Group II - serum urea determination)

[0169]

[0170]

[0171] Table 3 Effects on the body weight of mice (Group III - Blood lactic acid determination)

[0172]

[0173] Table 4 Effects on the body weight of mice (Group IV - Hepatic glycogen content determination)

[0174]

[0175] 2.2 Effects on the load-bearing swimming time of mice

[0176] Compared with the blank control group: The load-bearing swimming time of the medium-dose group of mice in Example 5 was prolonged compared with the blank control group, and the difference was significant (P < 0.05); The load-bearing swimming time of each dose group of mice in Comparative Example 2 and Comparative Example 3 was prolonged compared with the blank control group, and the difference was significant (P < 0.05), as shown in Table 5.

[0177] Comparison of the effects of prolonging the load-bearing swimming time among groups: Comparative Example 2 / Comparative Example 3 > Example 5 > Comparative Example 1.

[0178] Table 5 Effects on the load-bearing swimming time of mice

[0179]

[0180] Note: The statistical test value was the comparison of each formulation experimental group with the blank control group respectively. *, compared with the blank control group, p < 0.05

[0181] 2.3 Effects on the serum urea of mice

[0182] Compared with the blank control group: The serum urea of the high-dose group of Example 5, the medium-dose group of Comparative Example 1, and the low-dose and high-dose groups of Comparative Example 2 was significantly lower than that of the blank control group, and the difference was significant (P < 0.05), as shown in Table 6.

[0183] Comparison of the effects of reducing the serum urea after exercise among groups: Comparative Example 2 > Example 5 > Comparative Example 1 / Comparative Example 3.

[0184] Table 6 Effects on the serum urea of mice

[0185]

[0186]

[0187] Note: The statistical test value was the comparison of each formulation experimental group with the blank control group respectively. *, compared with the blank control group, p < 0.05

[0188] 2.4 Effects on the change of blood lactic acid in mice after exercise

[0189] The area under the blood lactic acid curve of each dose group showed no significant difference compared with the blank control group (P>0.05), and the results are shown in Table 7.

[0190] There was no obvious trend in the comparison of the effects of reducing the area under the blood lactic acid curve among groups.

[0191] Table 7 Effects on the changes of blood lactic acid in mice

[0192]

[0193]

[0194] 2.5 Effects on the liver glycogen reserve in mice

[0195] Compared with the blank control group: The liver glycogen reserves of the low-dose and high-dose groups of mice in Example 5 were significantly higher than those of the blank control group, with significant differences (P<0.05), as shown in Table 8.

[0196] Comparison of the effects of increasing the liver glycogen reserve in mice among groups: Example 5 > Comparative Example 3 > Comparative Example 2 > Comparative Example 1.

[0197] Table 8 Effects on the liver glycogen reserve in mice

[0198]

[0199]

[0200] Note: The statistical test values were the comparisons of each formulation experimental group with the blank control group respectively. *, compared with the blank control group, p<0.05

[0201] From the above data, it can be seen that Example 5, Comparative Example 2, and Comparative Example 3 have significant effects in improving the exercise endurance of mice; Example 5, Comparative Example 1, and Comparative Example 2 can effectively reduce the accumulation of urea in the body after exercise fatigue in mice; Example 5 has a positive effect on maintaining a high level of liver and muscle glycogen reserves. It shows that the composition with the dosage ratio in Example 5 of the present invention has the best effect in relieving physical fatigue.

[0202] In summary, Example 5 of the present invention can significantly extend the load-bearing swimming time of mice, can significantly increase the glycogen reserve in the liver of mice, and can significantly reduce the serum urea level after exercise in mice. Compared with the blank control group, the difference is significant (P<0.05). According to the evaluation criteria in the "Functional Test and Evaluation Methods for Health Foods (2023 Edition)", it has the function of relieving physical fatigue. It can be seen that various components in the composition provided by Example 5 of the present invention interact with each other in a specific and appropriate combination of compatibility doses, which can effectively enhance the exercise endurance of mice, reduce the accumulation of the metabolite urea in the serum, increase the glycogen content in the liver of mice, increase the body's energy reserve, thereby delaying the appearance of fatigue and achieving the effect of relieving physical fatigue.

[0203] Test Example 2

[0204] In order to further verify the characteristics of the composition of the present invention, an animal experiment study on the function of relieving physical fatigue was carried out on the composition obtained according to Example 5 (hereinafter referred to as the composition of the present invention) and the compositions obtained in Comparative Examples 4 and 5.

[0205] 1. Test purpose

[0206] In this test, by observing and detecting the load-bearing swimming time, serum urea content, blood lactic acid content, and liver glycogen content of mice, it is evaluated whether the composition of the present invention has the effect of relieving physical fatigue.

[0207] 2. Test method

[0208] 2.1 Test design basis

[0209] Referring to the "Functional Test and Evaluation Methods for Health Foods (2023 Edition)", relevant tests were carried out to detect the function of relieving physical fatigue. The swimming test is a recognized observation index for observing the anti-fatigue ability of animals. Therefore, the endurance of the body can be reflected by the length of the load-bearing swimming time of mice.

[0210] 2.2 Animals and grouping

[0211] 120 SPF-grade BALB / C female mice weighing 19-22 g (Beijing Vital River Laboratory Animal Technology Co., Ltd.), Beijing Laboratory Animal Quality Certificate No. 110011241109964256 (the experimental animal production license number is SYXK (Ji) 2020-003, and the experimental animal use license number is SCXK (Jing) 2021-0006), and the animal ethics number is N2024160.

[0212] 120 SPF-grade BALB / C female mice weighing 19-22 g (Beijing Huafukang Biotechnology Co., Ltd.), Beijing Laboratory Animal Quality Certificate No. 110322241103731852, and the animal ethics number is N2024166.

[0213] Select the animals that have passed the quarantine and randomly divide them into a negative control group, Comparative Example 1 group, Comparative Example 2 group, Low-dose group of the Example, Medium-dose group of the Example, and High-dose group of the Example according to body weight. Among them, the dosing dose is converted at 10 times the human recommended dose, which is the medium dose of the Example. The Low-dose, Medium-dose, and High-dose groups of the Example were given the composition of the present invention, and the dosing doses were 0.67, 1.33, and 2.67 raw herbs / kg (i.e., 0.29, 0.58, and 1.17 g extract / kg) respectively. The dosing doses of the Comparative Example 1 group and the Comparative Example 2 group were 1.33 and 0.67 g raw herbs / kg (i.e., 0.70 and 0.22 g extract / kg) respectively.

[0214] 2.3 Administration method

[0215] Gavage administration.

[0216] 2.4 Preparation and preservation of the test article

[0217] No preparation is required, and it is stored sealed and refrigerated.

[0218] 2.5 Administration of the test article

[0219] Select a disposable sterile syringe and gavage needle of appropriate specifications for gavage administration. The blank group was given the same volume of purified water. Administer the drug once a day for 30 consecutive days.

[0220] 2.6 Index detection

[0221] 2.6.1 Load-bearing swimming experiment

[0222] On the day after the last gavage administration, a lead sheet weighing 5% of the body weight was loaded at the root of the mouse's tail, and the mouse was placed in a swimming tank (about 50 cm × 50 cm × 40 cm) to swim. The water depth was not less than 30 cm, and the water temperature was 25°C ± 1.5°C. Record the time from the start of swimming to the death of the mouse (the mouse's head was completely submerged for 8 s, and if it could not surface, it was judged as the end point), that is, the load-bearing swimming time of the mouse. Precautions during swimming: ① Pay attention to maintaining the water temperature. Too low water temperature may cause muscle spasms and bring errors to the load-bearing swimming time; ② The number of mice swimming in each water tank should not be too many to avoid mutual collision and choking, and it is also inconvenient for researchers to observe; ③ Pay attention to the mouse's limbs throughout the process to keep it moving continuously. If the mouse is in a floating state with its limbs motionless, a glass rod can be stirred nearby to promote its movement.

[0223] 2.6.2 Serum urea detection

[0224] Thirty minutes after the last administration to the mice, they were made to swim without load in water at 30 °C for 90 min (to prepare a high urea model), and after a 60-min rest, blood was collected. Whole blood of about 0.5 mL was collected from the mice by eye socket enucleation (without adding anticoagulant). It was placed in a refrigerator at 4 °C for about 3 h. After the blood coagulated, it was centrifuged at 2000 r / min for 15 min, and the serum was taken for standby. The serum urea content was detected using an automatic biochemical analyzer.

[0225] 2.6.3 Detection of hepatic glycogen

[0226] The mice were sacrificed 30 min after the last administration. The liver was taken, rinsed with physiological saline, and then dried with filter paper. Exactly 200 mg of the liver was weighed, homogenized for 1 min, the homogenate was poured into a centrifuge tube, and centrifuged at 3000 r / min for 15 min. The supernatant was subjected to glycogen detection.

[0227] 2.6.4 Detection of blood lactic acid

[0228] Thirty minutes after the last administration to the mice, 30 μL of blood was collected from the inner canthus with a capillary tube, and then they swam without load in water at 30 °C for 10 min and then stopped. 30 μL of blood was collected respectively after swimming and after a 30-min rest, and then blood lactic acid detection was carried out.

[0229] 2.7 Statistical methods

[0230] The experimental data were analyzed using SPSS 20.0 statistical software, and the test levels were 0.05 and 0.01. The results were expressed as mean ± standard deviation First, a normality test was performed. For data conforming to a normal distribution, the mean comparison was carried out using one-way analysis of variance (One-Way ANOVA). If the variances were homogeneous, pairwise comparisons were performed using the least significant difference method (LSD). If the variances were not homogeneous, Dunnett’s T3 test was used for pairwise comparisons; if not conforming to a normal distribution, non-parametric tests were used; for count data, chi-square test or rank sum test was used.

[0231] 3. Results

[0232] 3.1 Load-bearing swimming time

[0233] As shown in Table 9, compared with the negative control group, the swimming time of the medium-dose and high-dose groups of the examples in the mice increased significantly (P < 0.01), that is, the swimming endurance of the mice was improved. Compared with Comparative Example Group 1, the swimming time of the medium-dose and high-dose groups of the examples in the mice increased significantly (P < 0.05). Compared with Comparative Example Group 2, the swimming time of the medium-dose group of the examples in the mice increased significantly (P < 0.05), and the swimming time of the high-dose group of the examples in the mice increased significantly (P < 0.01).

[0234] Table 9 Load-bearing swimming time, serum urea content, and hepatic glycogen content of each group

[0235]

[0236] Note: Compared with the negative control group: *P < 0.05, **P < 0.01; compared with Comparative Example 1 group, #p < 0.05, ##p < 0.01; compared with Comparative Example 2 group, △p < 0.05, △△p < 0.01.

[0237] 3.2 Serum urea content

[0238] As shown in Table 9, compared with the negative control group, the serum urea content of mice in Comparative Example 1 group, Comparative Example 2 group, and the low-dose group of the Examples decreased significantly (P < 0.05), and the serum urea content of mice in the medium-dose and high-dose groups of the Examples decreased significantly (P < 0.01).

[0239] 3.3 Liver glycogen content

[0240] As shown in Table 9, compared with the negative control group, the liver glycogen content of mice in Comparative Example 1 group and the low-dose group of the Examples increased significantly (P < 0.05), and the liver glycogen content of mice in Comparative Example 2 group, the medium-dose group of the Examples, and the high-dose group of the Examples increased significantly (P < 0.01).

[0241] 3.4 Blood lactic acid content

[0242] 3.4.1 Blood lactic acid content before exercise: As shown in Table 10, compared with the negative control group, the blood lactic acid content of mice in the medium-dose and high-dose groups of the Examples decreased significantly (P < 0.01). There was no statistical difference in other groups.

[0243] 3.4.2 Blood lactic acid content at 0 min after exercise: As shown in Table 10, compared with the negative control group, the blood lactic acid content of mice in the medium-dose and high-dose groups of the Examples decreased significantly (P < 0.05). Compared with Comparative Example 1 group, the blood lactic acid content of mice in the medium-dose group of the Examples decreased significantly (P < 0.05), and the blood lactic acid content of mice in the high-dose group of the Examples decreased significantly (P < 0.01). There was no statistical difference in other groups.

[0244] 3.4.3 Blood lactic acid content after resting for 20 min: As shown in Table 10, compared with the negative control group, the blood lactic acid content of mice in the high-dose group of the Examples decreased significantly (P < 0.01). Compared with Comparative Example 1 group, the blood lactic acid content of mice in the high-dose group of the Examples decreased significantly (P < 0.05). Compared with Comparative Example 2 group, the blood lactic acid content of mice in the high-dose group of the Examples decreased significantly (P < 0.01). There was no statistical difference in other groups.

[0245] 3.4.4 Area under the blood lactate curve: As shown in Table 10, compared with the negative control group, the area under the blood lactate curve of the mice in the medium-dose group of the examples was significantly reduced (P < 0.05), and the area under the blood lactate curve of the mice in the high-dose group of the examples was significantly reduced (P < 0.01). Compared with the control group 1, the area under the blood lactate curve of the mice in the high-dose group of the examples was significantly reduced (P < 0.05). Compared with the control group 2, the area under the blood lactate curve of the mice in the high-dose group of the examples was significantly reduced (P < 0.01). There were no statistical differences in other groups.

[0246] Table 10 Blood lactate content and area under the curve in each group

[0247]

[0248] Note: Compared with the negative control group: *P < 0.05, **P < 0.01; compared with the control group 1, #p < 0.05, ##p < 0.01; compared with the control group 2, △p < 0.05, △△p < 0.01.

[0249] 4. Conclusion

[0250] In this experiment, the weight-bearing swimming time, serum urea content, blood lactate content, and liver glycogen content of mice were detected to evaluate whether the composition of the present invention has the function of relieving physical fatigue. The experimental results showed that after the mice were continuously intragastrically administered for 30 days, the control group 1 and the control group 2 could increase the weight-bearing swimming time of the mice, increase the liver glycogen content, and reduce the serum urea content; the examples could increase the weight-bearing swimming time of the mice, increase the liver glycogen content, and reduce the serum urea content and the area under the blood lactate curve. The control group 1, the control group 2, and the examples have the effect of relieving physical fatigue, and the examples have the best effect. In terms of improving the exercise endurance of mice, alleviating muscle fatigue symptoms, enhancing the body's energy supply, and promoting rapid recovery after exercise, the examples of the present invention have shown obvious advantages compared with the control groups.

[0251] The composition provided by the present invention has the effect of relieving physical fatigue and can be applied to the preparation of health foods, functional foods or drugs with the effect of relieving physical fatigue to meet the daily needs of people's pursuit of health.

[0252] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements or improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition having the function of relieving physical fatigue, characterized in that, It consists of the following raw materials in parts by weight: Cuscuta chinensis 0.8 - 2.0 parts; Lycium barbarum 0.8 - 2.0 parts; Rubus chingii 0.2 - 1.0 parts; Schisandra chinensis 0.2 - 1.0 parts; Cassia obtusifolia 0.3 - 0.9 parts; Phyllanthus emblica 0.1 - 0.7 parts; Hovenia dulcis 0.1 - 0.7 parts; Gardenia jasminoides 0.05 - 0.35 parts; Chaenomeles sinensis 0.3 - 0.9 parts; Panax ginseng 0.5 - 1.5 parts and Cervus elaphus Linnaeus 0.5 - 1.5 parts.

2. The composition with the function of relieving physical fatigue according to claim 1, wherein, It consists of the following raw materials in parts by weight: Cuscuta chinensis 1.0 - 1.5 parts; Lycium barbarum 1.0 - 1.5 parts; Rubus chingii 0.3 - 0.9 parts; Schisandra chinensis 0.3 - 0.9 parts; Cassia obtusifolia 0.4 - 0.8 parts; Phyllanthus emblica 0.2 - 0.6 parts; Hovenia dulcis 0.2 - 0.6 parts; Gardenia jasminoides 0.15 - 0.3 parts; Chaenomeles sinensis 0.4 - 0.8 parts; Panax ginseng 0.8 - 1.2 parts and Cervus elaphus Linnaeus 0.8 - 1.2 parts.

3. The composition with the function of relieving physical fatigue according to claim 1, wherein, It consists of the following raw materials in parts by weight: Cuscuta chinensis 2.0 parts; Lycium barbarum 1.5 parts; Rubus chingii 1.0 parts; Schisandra chinensis 0.9 parts; Cassia obtusifolia 0.9 parts; Phyllanthus emblica 0.7 parts; Hovenia dulcis 0.6 parts; Gardenia jasminoides 0.35 parts; Chaenomeles sinensis 0.8 parts; Panax ginseng 1.5 parts and Cervus elaphus Linnaeus 1.2 parts.

4. The composition with the effect of relieving physical fatigue as claimed in claim 1, wherein, It consists of the following raw materials in parts by weight: Cuscuta chinensis 0.8 parts; Lycium barbarum 1.0 parts; Rubus chingii 0.2 parts; Schisandra chinensis 0.3 parts; Cassia obtusifolia 0.3 parts; Phyllanthus emblica 0.1 parts; Hovenia dulcis 0.2 parts; Gardenia jasminoides 0.05 parts; Chaenomeles sinensis 0.4 parts; Panax ginseng 0.5 parts and Cervus elaphus Linnaeus 0.8 parts.

5. The composition with the effect of alleviating physical fatigue according to claim 1, wherein It consists of the following raw materials in parts by weight: Cuscuta chinensis 1.3 parts; Lycium barbarum 1.3 parts; Rubus chingii 0.6 parts; Schisandra chinensis 0.6 parts; Cassia obtusifolia 0.6 parts; Phyllanthus emblica 0.4 parts; Hovenia dulcis 0.4 parts; Gardenia jasminoides 0.2 parts; Chaenomeles sinensis 0.6 parts; Panax ginseng 1.0 parts and Cervus elaphus Linnaeus 1.0 parts.

6. The composition with the function of relieving physical fatigue according to claim 1, wherein It consists of the following raw materials in parts by weight: Cuscuta chinensis 1.0 parts; Lycium barbarum 0.8 parts; Rubus chingii 0.3 parts; Schisandra chinensis 0.2 parts; Cassia obtusifolia 0.4 parts; Phyllanthus emblica 0.2 parts; Hovenia dulcis 0.1 parts; Gardenia jasminoides 0.15 parts; Chaenomeles sinensis 0.3 parts; Panax ginseng 0.8 parts and Cervus elaphus Linnaeus 0.5 parts.

7. The composition having the effect of relieving physical fatigue as described in claim 1, wherein It consists of the following raw materials in parts by weight: Cuscuta chinensis 1.5 parts; Lycium barbarum 2.0 parts; Rubus chingii 0.9 parts; Schisandra chinensis 1.0 parts; Cassia obtusifolia 0.8 parts; Phyllanthus emblica 0.6 parts; Hovenia dulcis 0.7 parts; Gardenia jasminoides 0.3 parts; Chaenomeles sinensis 0.9 parts; Panax ginseng 1.2 parts and Cervus elaphus Linnaeus 1.5 parts.

8. A method for preparing the composition having the effect of relieving physical fatigue according to any one of claims 1 to 7, characterized in that, It includes the following steps: Take the slices of Panax ginseng and Cervus elaphus Linnaeus and crush them separately to obtain ginseng powder and Cervus elaphus Linnaeus powder; Take Cuscuta chinensis, Lycium barbarum, Rubus chingii, Schisandra chinensis, Cassia obtusifolia, Phyllanthus emblica, Hovenia dulcis, Gardenia jasminoides and Chaenomeles sinensis, reflux and decoct them twice with water, filter and combine the filtrates to obtain an extract; Concentrate the extract under reduced pressure to a relative density of 1.02 - 1.12 to obtain an extract paste. After the extract paste is dried, a dry paste is obtained. After the dry paste is crushed, a dry paste powder is obtained; The obtained ginseng powder, Cervus elaphus Linnaeus powder and the dry paste powder together constitute the composition.

9. The preparation method of the composition with the function of relieving physical fatigue according to claim 8, characterized in that, Each reflux extraction and decoction lasts for 1.0 to 2.0 hours, and 7 to 11 times the amount of water is added for each reflux extraction and decoction; the temperature for vacuum concentration is 50 to 80 °C, and the vacuum degree is 0.04 to 0.09 MPa; the temperature for drying the extract is 110 to 120 °C, and the vacuum degree is 0.04 to 0.10 MPa.

10. Use of the composition with the function of alleviating physical fatigue according to any one of claims 1 to 7 in the preparation of functional foods, health foods or drugs.

11. A functional food, characterized in that, It includes the composition with the function of alleviating physical fatigue according to any one of claims 1 to 7.

12. A health food, characterized in that, It includes the composition with the function of alleviating physical fatigue according to any one of claims 1 to 7.

13. The health food according to claim 12, characterized in that, It further includes the following raw and auxiliary materials in parts by weight: 0.25 to 1.0 part of microcrystalline cellulose and 0.01 to 0.05 part of magnesium stearate.

14. The health food according to claim 12, characterized in that, The dosage form of the health food is granule, tablet or capsule.

15. The health food according to claim 14, characterized in that, The preparation method of the capsule of the health food includes the following steps: S1. Take the ginseng and pilose antler slices and pulverize them respectively to obtain ginseng powder and pilose antler powder, and irradiate them for sterilization; S2. Take dodder seeds, wolfberries, fructus rubi, schisandra chinensis, cassia seeds, phyllanthus emblica,hovenia dulcis, gardenia jasminoides and papaya, reflux extract and decoct them twice with water, filter and combine the filtrates to obtain an extract; S3. Concentrate the extract under reduced pressure to a relative density of 1.02 to 1.12 to obtain an extract paste. After the extract paste is dried, a dry paste is obtained. After the dry paste is pulverized, a dry paste powder is obtained; S4. Take the irradiated ginseng powder and pilose antler powder, mix them with microcrystalline cellulose and dry paste powder, granulate to obtain granules; S5. Take the obtained granules and mix them with magnesium stearate to obtain total mixed granules; S6. Take the obtained total mixed granules and fill them into capsules to obtain the product.

16. The health food according to claim 15, wherein In step S2, each reflux extraction and decoction lasts for 1.0 to 2.0 hours, and 7 to 11 times the amount of water is added for each reflux extraction and decoction; in step S3, the temperature for vacuum concentration is 50 to 80 °C, and the vacuum degree is 0.04 to 0.09 MPa; in step S3, the temperature for drying is 110 to 120 °C, and the vacuum degree is 0.04 to 0.10 MPa.

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