Composition and application thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a composition and application thereof. The invention relates to the field of cosmetics, in particular to a composition with the effects of removing yellowness and whitening and application of the composition to preparation of cosmetics and / or skin care products. The invention provides a composition. The composition comprises a carrier, a humectant, an antioxidant, a thickening agent and an active agent, wherein the active agent comprises a prepared rehmannia root extract, a rhizoma polygonati extract, a lysimachia christinae hance extract and a lily bulb extract. The applicant creatively discovers that the composition disclosed by the invention can have better effects of removing yellowness and whitening through the synergistic effect of all the components, and meanwhile, the safety risk is further reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products. Specifically, it relates to a composition and its uses; more specifically, it relates to a composition with the effects of reducing yellowish complexion and whitening, and its use for preparing cosmetics and / or skin care products. Background Art

[0002] With the improvement of people's requirements for beauty effects, higher requirements are put forward for both the whitening effect and safety of products. Existing whitening agents, such as chemical whitening agents like vitamin C, arbutin, etc., although having significant effects, are prone to cause skin irritation and allergic reactions. Whitening agents extracted from plants, such as licorice extract, ginseng extract, etc., although having relatively high safety, often have weak effects and require relatively high concentrations to achieve obvious whitening effects. Biotechnology whitening agents, such as superoxide dismutase (SOD), tyrosinase inhibitors obtained through genetic engineering, etc., although having high-efficiency whitening effects, have high costs and certain safety risks. Therefore, existing whitening agents either have relatively high safety but weak whitening effects, or have better whitening effects but may cause harm to skin health when used for a long time.

[0003] Therefore, there is a need in the art for a product that has better effects of reducing yellowish complexion and whitening, while further reducing safety risks, and finally can achieve the above effects at a relatively low concentration. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a composition with better effects of reducing yellowish complexion and whitening, while further reducing safety risks.

[0005] In view of this, in the first aspect of the present invention, the present invention provides a composition, comprising:

[0006] a carrier, a humectant, an antioxidant, a thickener, and an active agent;

[0007] wherein, the active agent includes: Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, and Lilium brownii extract.

[0008] The applicant has creatively found that using the composition of the present invention, through the synergistic effect of each component, it can have better effects of reducing yellowish complexion and whitening, while further reducing safety risks.

[0009] In some specific embodiments, the mass ratio of Rehmannia glutinosa extract:Polygonatum sibiricum extract:Lysimachia christinae extract:Lilium brownii extract is 1:(0.6 - 0.8):(0.2 - 0.4):(0.4 - 0.6).

[0010] Furthermore, the active agent further comprises one or more of astragalus extract, ganoderma extract, or white peony root extract.

[0011] Using the above-mentioned composition, its effects of reducing yellowness and whitening are better.

[0012] In some specific embodiments, the mass fraction concentration of the rehmannia root extract accounts for 0.5 - 3.0 wt% of the whole composition. For example, it accounts for 0.5 wt%, 1.0 wt%, 1.5 wt%, 2.0 wt%, 2.5 wt%, or 3.0 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.5 g, 1.0 g, 1.5 g, 2.0 g, 2.5 g, or 3.0 g of rehmannia root extract.

[0013] In some specific embodiments, the mass fraction concentration of the polygonatum extract accounts for 0.3 - 2.5 wt% of the whole composition. For example, it accounts for 0.3 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, 2.0 wt%, or 2.5 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.3 g, 0.5 g, 1.0 g, 1.5 g, 2.0 g, or 2.5 g of polygonatum extract.

[0014] In some specific embodiments, the mass fraction concentration of the lysimachia christinae extract accounts for 0.1 - 1.2 wt% of the whole composition. For example, it accounts for 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.5 wt%, 1.0 wt%, or 1.2 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.1 g, 0.2 g, 0.3 g, 0.5 g, 1.0 g, or 1.2 g of lysimachia christinae extract.

[0015] In some specific embodiments, the mass fraction concentration of the lily extract accounts for 0.2 - 1.8 wt% of the whole composition. For example, it accounts for 0.2 wt%, 0.3 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, or 1.8 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.2 g, 0.3 g, 0.5 g, 1.0 g, 1.5 g, or 1.8 g of lily extract.

[0016] In some specific embodiments, the mass fraction concentration of the astragalus extract accounts for 0.05 - 0.8 wt% of the whole composition. For example, it accounts for 0.05 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.5 wt%, or 0.8 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.05 g, 0.1 g, 0.2 g, 0.3 g, 0.5 g, or 0.8 g of astragalus extract.

[0017] In some specific embodiments, the mass fraction concentration of the Ganoderma lucidum extract accounts for 0.03 - 0.5 wt% of the whole composition. For example, it accounts for 0.03 wt%, 0.05 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, or 0.5 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.03 g, 0.05 g, 0.1 g, 0.2 g, 0.3 g, or 0.5 g of Ganoderma lucidum extract.

[0018] In some specific embodiments, the mass fraction concentration of the Paeonia lactiflora extract accounts for 0.1 - 0.6 wt% of the whole composition. For example, it accounts for 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, or 0.6 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 0.1 g, 0.2 g, 0.3 g, 0.4 g, 0.5 g, or 0.6 g of Paeonia lactiflora extract.

[0019] Furthermore, the carrier of the present invention includes one or more of water, oils and fats, or waxes.

[0020] For example, any amount of water can be included. As the water, the water used in cosmetics, quasi-drugs, etc. can be used. For example, ion-exchanged water, distilled water, ultrapure water, tap water, etc. can be used.

[0021] For example, the oils and fats that can be included are one or more of jojoba oil, peony seed oil, sunflower seed oil, caprylic / capric triglyceride, shea butter, cyclopentasiloxane, squalane, or olive oil.

[0022] For example, the waxes that can be included are one or more of microcrystalline wax, beeswax, candelilla wax, carnauba wax, rice bran wax, lanolin, acetylated lanolin, lanolin oil, hydrogenated lanolin, jojoba wax, sugarcane wax, or isopropyl lanolate.

[0023] Furthermore, the mass fraction of the carrier accounts for 1 - 40 wt% of the whole composition. For example, it accounts for 1 wt%, 5 wt%, 10 wt%, 20 wt%, 30 wt%, or 40 wt% of the whole composition. For example, every 100 g of the composition of the present invention contains 1 g, 5 g, 10 g, 20 g, 30 g, or 40 g of the carrier.

[0024] Furthermore, the humectant of the present invention includes one or more of glycerol, propylene glycol, 1,3-butanediol, xylitol, sorbitol, hyaluronic acid, sodium lactate, pyrrolidone carboxylate, short-chain soluble collagen, Achillea millefolium extract, or polyethylene glycol.

[0025] Further, the mass fraction of the humectant accounts for 5-30 wt% of the whole composition. For example, it accounts for 5 wt%, 10 wt%, 20 wt%, or 30 wt% of the whole composition. For example, in every 100 g of the composition of the present invention, there are 5 g, 10 g, 20 g, or 30 g of the humectant.

[0026] Further, the antioxidant includes one or more of vitamin E (tocopherol), disodium EDTA (chelating agent), sodium metabisulfite, or green tea extract.

[0027] Further, the mass fraction of the antioxidant accounts for 0.1-5 wt% of the whole composition. For example, it accounts for 0.1 wt%, 0.5 wt%, 1 wt%, 2 wt%, 3 wt%, or 5 wt% of the whole composition. For example, in every 100 g of the composition of the present invention, there are 0.1 g, 0.5 g, 1 g, 2 g, 3 g, or 5 g of the antioxidant.

[0028] Further, the thickener includes one or more of gum arabic, carrageenan, carrageenin, tragacanth, locust bean gum, casein, dextrin, gelatin, sodium pectate, sodium alginate, methyl cellulose, ethyl cellulose, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, PVA, PVM, PVP, sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, cellulose sulfate dimethyl dialkyl ammonium, xanthan gum, magnesium aluminum silicate, bentonite, lithium montmorillonite, propolis, or silica anhydride.

[0029] Further, the mass fraction of the thickener accounts for 0.01-3 wt% of the whole composition. For example, it accounts for 0.1 wt%, 0.5 wt%, 1 wt%, 2 wt%, or 3 wt% of the whole composition. For example, in every 100 g of the composition of the present invention, there are 0.1 g, 0.5 g, 1 g, 2 g, or 3 g of the antioxidant.

[0030] In the second aspect, the present invention provides the use of the above composition for preparing cosmetics and / or skin care products.

[0031] Further, the cosmetics and / or skin care products can be for facial use or for lip use.

[0032] Further, the mass fraction of the composition accounts for 1-100% in the cosmetics and / or skin care products, and can account for 1%, 5%, 10%, 20%, 30%, 40%, 50%, 80%, 100% in the cosmetics and / or skin care products.

[0033] For example, each 100 g of the cosmetic and / or skin care product may include 1 g to 100 g of the composition of the present invention, such as 1 g, 5 g, 10 g, 20 g, 30 g, 40 g, 50 g, 80 g, 100 g of the composition of the present invention.

[0034] In a third aspect, the present invention provides a cosmetic and / or skin care product, which includes the composition as described above.

[0035] Furthermore, the mass fraction of the composition accounts for 1 to 100% in the cosmetic and / or skin care product, and may account for 1%, 5%, 10%, 20%, 30%, 40%, 50%, 80%, 100% in the cosmetic and / or skin care product.

[0036] For example, each 100 g of the cosmetic and / or skin care product may include 1 g to 100 g of the composition of the present invention, such as 1 g, 5 g, 10 g, 20 g, 30 g, 40 g, 50 g, 80 g, 100 g of the composition of the present invention.

[0037] Furthermore, the cosmetic may be makeup, such as cushion foundation, liquid foundation, loose powder, pressed powder, makeup primer, makeup base, lipstick, blush, contour powder, eyeshadow, etc.

[0038] Furthermore, the product may be used on the face or on the lips.

[0039] The products for facial use may be eyeshadow, makeup remover, facial cleanser, essence, lotion, emulsion, cream, etc.

[0040] The products for lip use may be lipstick, lip glaze, lip balm, lip color, lip gloss, lip cream, etc.

[0041] As the dosage forms that can be used as cosmetics, stick-shaped, dish-shaped, ointment-shaped and other dosage forms can be adopted.

[0042] In the present invention, in the cosmetic, in addition to the above components, any components commonly used in cosmetics and pharmaceuticals may also be formulated. As the optional components, the following components can be mentioned, and one or more than two can be formulated as long as the effects of the present invention are exerted.

[0043] In some specific embodiments, the cosmetic of the present invention further includes one or more of an emollient, a filler, a colorant, a preservative, an adhesive, or an emulsifier.

[0044] In some specific embodiments, the preservatives of the present invention may include, for example, methyl paraben, ethyl paraben, butyl paraben and other parabens, benzoic acid, salicylic acid, sorbic acid, parachlorometacresol, hexachlorophene, benzalkonium chloride, chlorhexidine, trichlorocarbanilide, photosensitizer, phenoxyethanol, etc.

[0045] In some specific embodiments, the emulsifiers of the present invention may include non-ionic surfactants and amphiphilic polymers, such as (acrylate / stearyl alcohol polyether-20) copolymer, hydrophobically modified alkyl cellulose, hydrogenated lecithin, sorbitan laurate, sorbitan palmitate, sorbitan oleate, sorbitan stearate, sorbitan isostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan tristearate, sorbitan sesquiisostearate.

[0046] In some specific embodiments, the skin care product of the present invention is a cream, comprising: deionized water, glycerin, shea butter, beeswax, tocopheryl acetate, xanthan gum, Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, Lilium brownii extract.

[0047] In some specific embodiments, the skin care product of the present invention is a lotion, comprising: deionized water, propylene glycol, jojoba oil, caprylic / capric triglyceride, carbomer 940, triethanolamine, Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, Lilium brownii extract, Paeonia lactiflora extract.

[0048] In some specific embodiments, the skin care product of the present invention is a serum, comprising: deionized water, glycerin, squalane, xanthan gum, ascorbyl glucoside, Rehmannia glutinosa extract, Lysimachia christinae extract, Polygonatum sibiricum extract, Lilium brownii extract, Paeonia lactiflora extract, Astragalus membranaceus extract, Ganoderma lucidum extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is a graph showing the results of the safety test of the composition of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0050] The present invention will be specifically described below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be presented more clearly therefrom. Those skilled in the art should understand that these specific embodiments and examples are for illustrating the present invention, rather than limiting the present invention.

[0051] The "ranges" disclosed in the present application are defined in the form of lower and upper limits. A given range is defined by selecting a lower limit and an upper limit, and the selected lower limit and upper limit define the boundary of a particular range. The ranges defined in this way can include or exclude the end values, and can be combined arbitrarily, that is, any lower limit can be combined with any upper limit to form a range.

[0052] In this application, unless otherwise specified, the numerical range "a - b" represents an abbreviated representation of any real number combination between a and b, where both a and b are real numbers. For example, the numerical range "0 - 5" means that all real numbers between "0 - 5" are fully listed herein, and "0 - 5" is just an abbreviated representation of these numerical combinations. Additionally, when stating that a certain parameter is an integer ≥2, it is equivalent to disclosing that the parameter is, for example, the integer 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0053] If there is no special instruction, all embodiments and optional embodiments of this application can be combined with each other to form new technical solutions.

[0054] If there is no special instruction, all technical features and optional technical features of this application can be combined with each other to form new technical solutions.

[0055] If there is no special instruction, all steps of this application can be carried out sequentially or randomly, preferably sequentially. For example, the method includes steps (a) and (b), which means that the method can include steps (a) and (b) carried out sequentially, or can also include steps (b) and (a) carried out sequentially. For example, it is mentioned that the method may further include step (c), which means that step (c) can be added to the method in any order. For example, the method can include steps (a), (b), and (c), or can also include steps (a), (c), and (b), or can also include steps (c), (a), and (b), etc.

[0056] The term

[0057] "XX extract" refers to an extract obtained by conventional methods, such as alcohol extraction, water extraction, etc., to obtain its corresponding active substances. For example, in this application, Rehmannia glutinosa and Polygonatum sibiricum are subjected to gradient percolation with 50 - 70% ethanol (40 - 60°C) to enrich the iridoid glycosides and polysaccharides of the Rehmannia glutinosa extract and Polygonatum sibiricum extract; Lysimachia christinae and Lilium brownii are subjected to subcritical water extraction (120 - 150°C, 3 - 5 MPa) to retain the thermosensitive flavonoids and saponins of the Lysimachia christinae extract and Lilium brownii extract. Astragalus membranaceus is subjected to gradient percolation with 60 - 75% ethanol (45 - 55°C) to retain astragaloside IV and astragalus polysaccharides; Ganoderma lucidum is subjected to dynamic reflux extraction with 90 - 95% ethanol (65 - 75°C) to retain triterpenoids (calculated as Ganoderic acid A + B); Paeonia lactiflora is extracted with a subcritical propylene glycol - water system (110 - 130°C, 2 - 4 MPa) to retain paeoniflorin.

[0058] Hereinafter, embodiments of the present application will be described. The embodiments described below are exemplary and are only used to explain the present application, and should not be construed as a limitation to the present application. For those not specified in the embodiments regarding specific technologies or conditions, they shall be carried out according to the technologies or conditions described in the literature in this field or according to the product specifications. For reagents or instruments without indicating the manufacturer, they are all conventional products that can be obtained through commercial purchases.

[0059] Example 1

[0060] The carrier uses water and 4 wt% jojoba oil, the moisturizer uses 5 wt% glycerin, the antioxidant uses 0.3 wt% tocopheryl acetate, the thickener uses 0.1 wt% xanthan gum, and the active agent is configured with the following extracts (all components are calculated based on 100% of the composition weight, and the final balance is made up to 100% with water):

[0061] 2 wt% Rehmannia glutinosa extract, 1.6 wt% Polygonatum sibiricum extract, 0.8 wt% Lysimachia christinae extract, 1.2 wt% Lilium brownii extract.

[0062] Example 2

[0063] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0064] 0.5 wt% Rehmannia glutinosa extract, 0.3 wt% Polygonatum sibiricum extract, 0.1 wt% Lysimachia christinae extract, 0.3 wt% Lilium brownii extract, 0.1 wt% Paeonia lactiflora extract.

[0065] Example 3

[0066] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0067] 3 wt% Rehmannia glutinosa extract, 2.4 wt% Polygonatum sibiricum extract, 1.2 wt% Lysimachia christinae extract, 1.8 wt% Lilium brownii extract, 0.6 wt% Paeonia lactiflora extract, 0.8 wt% Astragalus membranaceus extract.

[0068] Example 4

[0069] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0070] 1 wt% Rehmannia glutinosa extract, 0.6 wt% Polygonatum sibiricum extract, 0.2 wt% Lysimachia christinae extract, 0.4 wt% Lilium brownii extract, 0.3 wt% Paeonia lactiflora extract, 0.05 wt% Astragalus membranaceus extract, 0.2 wt% Ganoderma lucidum extract.

[0071] Comparative Example 1

[0072] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0073] 2 wt% Rehmannia glutinosa extract, 1.6 wt% Polygonatum sibiricum extract, 0.8 wt% Lysimachia christinae extract, 1.2 wt% Morus alba root bark extract (the Morus alba root bark extract replaces the Lilium brownii extract).

[0074] Comparative Example 2

[0075] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0076] 2 wt% Rehmannia glutinosa extract, 1.6 wt% Polygonatum sibiricum extract, 0.8 wt% Salvia miltiorrhiza extract, 1.2 wt% Lilium brownii extract (the Salvia miltiorrhiza extract replaces the Lysimachia christinae extract).

[0077] Comparative Example 3

[0078] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0079] 2 wt% Rehmannia glutinosa recens extract, 1.6 wt% Polygonatum sibiricum extract, 0.8 wt% Lysimachia christinae extract, 1.2 wt% Lilium brownii extract (the Rehmannia glutinosa recens extract replaces the Rehmannia glutinosa extract).

[0080] Comparative Example 4

[0081] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0082] 2 wt% Rehmannia glutinosa extract, 1.6 wt% Cremastra appendiculata extract, 0.8 wt% Lysimachia christinae extract, 1.2 wt% Lilium brownii extract (the Cremastra appendiculata extract replaces the Polygonatum sibiricum extract).

[0083] Comparative Example 5

[0084] On the basis that the rest of Example 1 remains unchanged, the active agent is configured with the following extracts:

[0085] 2 wt% Rhaponticum uniflorum extract, 1.6 wt% Polygonatum sibiricum extract, 0.8 wt% Lysimachia christinae extract, 1.2 wt% Lilium brownii extract (the Rhaponticum uniflorum extract replaces the Rehmannia glutinosa extract).

[0086] The compositions prepared in Examples 1 to 4 and Comparative Examples 1 to 5 were experimented by the following method, and the melanin inhibition rate (%), DPPH scavenging IC 50 , AGEs inhibition EC 50 are shown in Table 1 below.

[0087] Antioxidant experiment method:

[0088] DPPH (a stable nitrogen-centered free radical) is used to evaluate the antioxidant capacity of samples. Due to its stable free radical properties, DPPH can capture other free radicals, and by observing the changes in the chemical reaction rate, the free radical scavenging ability of the samples can be judged. The antioxidant effect of the samples is evaluated through the DPPH free radical scavenging rate.

[0089] The experimental procedure includes weighing 0.8 mg of DPPH, adding anhydrous ethanol to dissolve it and making up the volume to 10 mL in a volumetric flask, and preparing the DPPH concentration to be 2×10 -4 mol / L; store it in the dark at 0 - 4 °C, prepare it freshly before use, and it is valid within 4 h. Solid samples are dissolved in anhydrous ethanol / ultrapure water / 10% DMSO (a solvent blank control needs to be set for 10% DMSO), and liquid samples are diluted with anhydrous ethanol / ultrapure water / 10% DMSO. The concentration is designed according to the actual experimental requirements and prepared freshly before use. After thorough mixing, react in the dark at room temperature for 30 min, and measure the absorbance values of all samples at 517 nm using a microplate reader. DPPH free radical scavenging rate (%) = [(B + C) - A] / B * 100.

[0090] Anti-glycation experimental method:

[0091] The non-enzymatic glycosylation (NEG) reaction refers to the combination of reducing sugars with the free amino groups of proteins to form advanced glycation end products (AGEs), which are considered to be the main cause of skin yellowing. By inhibiting this process, the brightness of the skin can be improved. The experiment uses the BSA-fructose in vitro system and measures the AGEs content by fluorescence quantitative method to evaluate the anti-glycation effect of the samples.

[0092] The experimental procedure includes pouring 0.5 g of collagen (bovine serum albumin) into a 50 mL EP tube and adding PBS to 25 ml. Dissolve 2.25 g of fructose in PBS to make up the volume to 25 ml. The bovine serum albumin solution and the fructose solution are uniformly mixed in a 1:1 ratio to prepare the BSA-fructose reaction solution. Solid samples are dissolved in ultrapure water / 10% DMSO (a solvent blank control needs to be set for 10% DMSO), and liquid samples are diluted with ultrapure water / 10% DMSO. The concentration is designed according to the actual experimental requirements and prepared freshly before use. The samples are filtered through a 0.22 μm CA filter membrane. Incubate in the dark in a 37 °C incubator for 7 days. Detect the fluorescence intensity of each group on the 7th day, with the excitation wavelength of 370 nm and the emission wavelength of 440 nm. AGEs inhibition rate (%) = [(OD control group - OD control blank) - (OD sample group - OD sample blank)] / (OD control group - OD control blank) * 100%.

[0093] Whitening experimental method:

[0094] Mouse melanoma cells are commonly used as a cell model for studying melanogenesis. By analyzing the changes in the melanin content in cells, the whitening efficacy of cosmetic raw materials can be evaluated, and thus effective whitening ingredients can be screened out. Mouse melanoma cells are inoculated into a culture dish, and after treatment, a microplate reader is used to measure the changes in the melanin content of the cells to assess the whitening potential of the sample.

[0095] The experimental steps include cell seeding: Discard the medium in the T25 culture flask, wash it twice with PBS, add 1 mL of 0.25% trypsin, place it in a CO2 incubator for digestion for 2 min. After observing the cells detaching from the culture flask under a microscope, 1 mL of complete medium can be added to terminate the digestion. Transfer the cell suspension to a 15 mL centrifuge tube, centrifuge at 1000 rpm for 4 min, and discard the supernatant. After resuspending with 1 mL of complete medium, cell counting is performed. Dilute the cell suspension to a density of 1.5×10 5 cells / mL and inoculate into a 6-well plate, 2 mL / well. After inoculation, place it in an incubator at 37°C and 5% CO2 for culture for 24 h ± 2 h; Sample addition: After culturing for 24 h, replace the cell culture medium with the culture medium containing the test sample, 2 mL per well, and continue to culture for 48 h; Detection: When the cell fusion rate reaches more than 90%, measure the melanin content of the cells. Wash the cells twice with PBS, aspirate the PBS, add 200 μL of 0.25% trypsin to each well to digest the cells, place it in a CO2 incubator for digestion for 5 minutes. Add 1 mL of PBS to pipette the cells, collect the cells into a 1.5 mL centrifuge tube, and centrifuge at 10000 rpm for 5 minutes. Spin dry, try to aspirate as much PBS residue in the centrifuge tube as possible. Add 250 μL of melanin extraction solution to each tube, shake well, place it in a water bath at 80°C for heating for 30 min until the cells are completely dissolved, then cool and centrifuge the liquid droplets on the lower wall moderately, pipette well, aspirate 100 μL of the solution from each centrifuge tube, transfer it to a 96-well plate, and use a microplate reader to detect the absorbance at 490 nm for each well. The calculation formula is as follows: Relative melanin content = (OD value of the test sample group - OD value of the melanin extraction solution) / (OD value of the negative control group - OD value of the melanin extraction solution) × 100%.

[0096] Table 1. Efficacy of the composition of the present invention

[0097]

[0098]

[0099] As can be seen from the data in Table 1, the melanin inhibition rates of Examples 1 to 4 are much higher than those of Comparative Examples 1 to 5, indicating the specificity of the four core components of the present invention. Replacing any one of them cannot achieve good melanin inhibition effects. The antioxidant properties, namely DPPH scavenging and anti-glycation AGEs inhibition, also show the same efficacy. Examples 1 to 4 have lower effective concentrations compared to Comparative Examples 1 to 5, that is, the same effect can be obtained at a lower concentration.

[0100] Preparation of the cosmetic composition of the present invention

[0101] The formula of the cream is shown in Table 2 and is prepared according to the following method:

[0102] Preparation of the oil phase: shea butter + beeswax, heated to 65°C and melted; preparation of the aqueous phase: water + glycerol + xanthan gum, stirred and dissolved at 75°C; emulsification: the oil phase was slowly added to the aqueous phase, and emulsified with a homogenizer at 3000 rpm for 5 minutes; cooling addition: tocopheryl acetate was added at 50°C; four active extracts were added at 40°C and stirred until homogeneous; filling: filled below 35°C and sealed in the dark.

[0103] Table 2, Cream formula

[0104]

[0105]

[0106] The formula of the lotion is shown in Table 3 and is prepared according to the following method:

[0107] Aqueous phase: water + propylene glycol + carbomer, dispersed and heated to 75°C; oil phase: jojoba oil + caprylic / capric triglyceride, melted at 65°C; emulsification: the oil phase was added to the aqueous phase, and emulsified with a homogenizer at 2500 rpm for 3 minutes; neutralization: cooled to 50°C, and triethanolamine was added dropwise to adjust the pH to 5.5; addition of active substances: the active extract was added at 40°C and stirred at low speed for 10 minutes.

[0108] Table 3, Lotion formula

[0109]

[0110]

[0111] The formula of the essence is shown in Table 4 and is prepared according to the following method:

[0112] Pre-dissolved colloid: water + glycerol + xanthan gum, stirred until transparent at 45°C; addition of oil: squalane was slowly added and mixed with a homogenizer at 1500 rpm for 2 minutes; functional addition: AA2G was added at 30°C and stirred until dissolved; three active extracts were added at 25°C and stirred in the dark for 15 minutes; filtration and filling: filtered through a 0.22 μm filter membrane and filled with nitrogen.

[0113] Table 4, Essence Formula

[0114]

[0115] Safety Tests of the Compositions and Cosmetics of the Present Invention

[0116] 1) Chicken Embryo

[0117] Test method: According to SN / T2329-2009 "Chorioallantoic Membrane Test of Cosmetics for Eye Irritation / Corrosion". By the endpoint evaluation method, 0.3 ml of the sample was dropped onto the CAM. After acting for 3 min, the test substance on the CAM membrane was gently rinsed with physiological saline to observe the occurrence of bleeding, blood coagulation, and vascular lysis, and photos were taken. At the same time, negative controls of 0.9% NaCl and positive controls of 0.2%, 0.3%, and 0.5% sodium hydroxide solutions were set up.

[0118] The composition of Example 1 was used for the experiment, and the results are as Figure 1 shown. It can be seen from Figure 1 that the composition of the present invention is non-irritating to chicken embryos, indicating good safety.

[0119] 2) Patch Test

[0120] Test method: Qualified patch test equipment was selected. By the closed patch test method, 0.020 - 0.025 ml of the test substance was placed in the patch test device, and the outer surface was applied to the flexor side of the forearm of the subject with a low-allergy tape. After 24 hours, the test substance was removed, and the skin reactions were observed at 0.5, 24, and 48 hours after removal, and the results were recorded according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0121] The creams, lotions, and essences prepared from the compositions of Examples 2 - 4 were used for the experiment respectively. The test results showed that 0 cases of skin adverse reactions occurred among 30 people, indicating the safety of the present invention.

Claims

1. A composition, comprising: A carrier, a humectant, an antioxidant, a thickener, and an active agent; Among them, the active agent includes: Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, and Lilium brownii extract.

2. The composition according to claim 1, wherein The mass ratio of the Rehmannia glutinosa extract: Polygonatum sibiricum extract: Lysimachia christinae extract: Lilium brownii extract is 1:(0.6 - 0.8):(0.2 - 0.4):(0.4 - 0.6).

3. The composition according to claim 1 or 2, characterized in that, The active agent also includes one or more of Astragalus membranaceus extract, Ganoderma lucidum extract, or Paeonia lactiflora extract.

4. The composition according to claim 3, characterized in that, The mass fraction concentration of the Rehmannia glutinosa extract accounts for 0.5 - 3.0 wt% of the whole composition, and / or the mass fraction concentration of the Polygonatum sibiricum extract accounts for 0.3 - 2.5 wt% of the whole composition, and / or the mass fraction concentration of the Lysimachia christinae extract accounts for 0.1 - 1.2 wt% of the whole composition, and / or the mass fraction concentration of the Lilium brownii extract accounts for 0.2 - 1.8 wt% of the whole composition, and / or the mass fraction concentration of the Astragalus membranaceus extract accounts for 0.05 - 0.8 wt% of the whole composition, and / or the mass fraction concentration of the Ganoderma lucidum extract accounts for 0.03 - 0.5 wt% of the whole composition, and / or the mass fraction concentration of the Paeonia lactiflora extract accounts for 0.1 - 0.6 wt% of the whole composition.

5. The composition according to claim 1, wherein The carrier includes one or more of water, oil, or wax.

6. The composition according to claim 5, wherein The oil includes one or more of jojoba oil, peony seed oil, sunflower seed oil, caprylic / capric triglyceride, shea butter, cyclopentasiloxane, squalane, or olive oil; and / or the wax includes one or more of microcrystalline wax, beeswax, candelilla wax, carnauba wax, rice bran wax, lanolin, acetylated lanolin, lanolin oil, hydrogenated lanolin, jojoba wax, sugarcane wax, or isopropyl lanolate.

7. The composition according to claim 1, wherein The humectant includes one or more of glycerol, propylene glycol, 1,3 - butanediol, xylitol, sorbitol, hyaluronic acid, sodium lactate, pyrrolidone carboxylate, short - chain soluble collagen, Achillea millefolium extract, or polyethylene glycol; and / or the antioxidant includes one or more of vitamin E (tocopherol), EDTA disodium (chelating agent), sodium metabisulfite, or green tea extract; and / or the thickener includes one or more of gum arabic, carrageenan, carrageenan, tragacanth gum, carob gum, casein, dextrin, gelatin, sodium pectate, sodium alginate, methyl cellulose, ethyl cellulose, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, PVA, PVM, PVP, sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, dialkyldimethylammonium sulfate cellulose, xanthan gum, magnesium aluminum silicate, bentonite, lithium montmorillonite, propolis, or silicon anhydride.

8. The use of the composition according to any one of claims 1 - 7 for preparing cosmetics and / or skin care products.

9. A cosmetic and / or skin care product, comprising the composition according to any one of claims 1 - 7.

10. The cosmetic and / or skin care product according to claim 9, wherein the cosmetic and / or skin care product is a cream, comprising: Deionized water, glycerol, shea butter, beeswax, tocopheryl acetate, xanthan gum, Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, Lilium brownii extract; or The cosmetic and / or skin care product is a lotion, comprising: deionized water, propylene glycol, jojoba oil, caprylic / capric triglyceride, carbomer 940, triethanolamine, Rehmannia glutinosa extract, Polygonatum sibiricum extract, Lysimachia christinae extract, Lilium brownii extract, Paeonia lactiflora extract; or The cosmetic and / or skin care product is a serum, comprising: deionized water, glycerol, squalane, xanthan gum, ascorbyl glucoside, Rehmannia glutinosa extract, Lysimachia christinae extract, Polygonatum sibiricum extract, Lilium brownii extract, Paeonia lactiflora extract, Astragalus membranaceus extract, Ganoderma lucidum extract.

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