Spray for pets and preparation method thereof

Abstract

The invention relates to a pet spray and a preparation method thereof. The spray for pets comprises the following raw material components: a traditional Chinese medicine component, a western medicine component and a pH buffer solution, the traditional Chinese medicine components comprise the following components in parts by weight: 0.1-1 part of tea polyphenol, 1-15 parts of chlorogenic acid and 0.1-1 part of menthol; the western medicine component accounts for 1-15 parts and comprises the following components in parts by weight: 1-10 parts of ornidazole, 0.1-2 parts of lincomycin, 0.1-0.5 part of mometasone furoate and 0.1-2 parts of vitamin B2; and the pH buffer solution accounts for 58-96 parts. The spray for the pets contains the traditional Chinese medicine components, the western medicine components and the buffer solution, the synergistic effect between the traditional Chinese medicine components and the western medicine components can be fully exerted, and when the spray is used for preventing and treating oral diseases, the effective medicine utilization degree is high, the effect is good, and drug resistance is not prone to being generated.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary drug preparation, and particularly relates to a pet spray and a preparation method thereof. Background Art

[0002] In recent years, the number of household pets has increased significantly, and some pets have become members of the family. However, due to various pathogenic factors and / or diet, oral diseases such as oral ulcers, gingivitis, periodontitis, glossitis, dental calculus, and bad breath in pets occur, leading to reduced food intake, weight loss, incomplete chewing, digestive system diseases, and decreased immunity in pets.

[0003] Currently, the clinical treatment and preventive measures for oral diseases are limited, mainly including dental cleaning, oral antiviral drugs, and oral antibacterial drugs, which have achieved certain effects. However, oral diseases are local diseases. When using oral drugs for systemic treatment, the utilization rate of effective drugs is low and the dosage is large. At the same time, after treating with oral antiviral drugs and / or antibacterial drugs until the infection is eliminated, there are often inflammatory symptoms, indicating that oral inflammation is often more difficult to treat than the infection itself. In addition, due to virus mutation and the increased drug resistance of bacteria and fungi, the recurrence rate is high, resulting in chronic infections.

[0004] Therefore, from the perspective of traditional Chinese medicine, it is of great significance to develop a pet spray for preventing and treating pet oral diseases. Summary of the Invention

[0005] In view of this, it is necessary to provide a pet spray and a preparation method thereof to solve the technical problems of low utilization rate of effective drugs, poor effect, and easy generation of drug resistance in the existing methods for preventing and treating oral diseases in the prior art.

[0006] The first aspect of the present invention provides a pet spray, and its raw material components include: traditional Chinese medicine components, western medicine components, and a pH buffer solution; by weight percentage,

[0007] The traditional Chinese medicine components account for 2-27 parts, and include: 0.1-1 part of tea polyphenols, 1-15 parts of chlorogenic acid, and 0.1-1 part of menthol;

[0008] The western medicine components account for 1-15 parts, and include: 1-10 parts of ornidazole, 0.1-2 parts of lincomycin, 0.1-0.5 parts of mometasone furoate, and 0.1-2 parts of vitamin B2;

[0009] The pH buffer solution accounts for 58-96 parts.

[0010] Further, the traditional Chinese medicine components account for 6-27 parts, the western medicine components account for 1.5-14 parts, and the pH buffer solution accounts for 61-87 parts.

[0011] Furthermore, the mass ratio of the traditional Chinese medicine components to the western medicine components is (0.4 - 4.5):1.

[0012] Furthermore, the mass ratio of chlorogenic acid to menthol is 1:(0.01 - 0.3).

[0013] Furthermore, the pH of the pet spray is controlled to be 6.5 - 7.5.

[0014] Furthermore, the pH buffer solution is phosphate buffer solution and / or carbonate buffer solution.

[0015] Furthermore, in the phosphate buffer solution, the mass fraction of disodium hydrogen phosphate is 0.454 - 1.945 wt%, and the mass fraction of sodium dihydrogen phosphate is 0.756 - 2.016 wt%.

[0016] The second aspect of the present invention provides a preparation method of a pet spray, comprising the following steps: proportioning raw materials according to the above ratios, and mixing evenly to obtain a solution-type pet spray.

[0017] The pharmacodynamic functions of the main raw materials of the present invention are as follows:

[0018] Tea polyphenols are the general term for polyphenolic substances in tea, and its main components are: flavanones, anthocyanins, flavonols, leucoanthocyanins, phenolic acids and condensed phenolic acids, etc. Tea polyphenols have various physiological activities such as antioxidant, anti-radiation, anti-aging, lipid-lowering, blood sugar-lowering, antibacterial and enzyme-inhibiting; in the medical field, tea polyphenols have the following effects: blood pressure-lowering, anticoagulation, lipid-lowering and obesity prevention, prevention and treatment of cardiovascular diseases such as arteriosclerosis and thrombus formation, blood sugar-lowering, prevention and treatment of diabetes, bactericidal and antiviral; prevention and treatment of gastrointestinal, respiratory, influenza and other diseases; prevention and treatment of hepatitis, anti-aging and enhancement of immune function; tooth health, prevention and treatment of periodontitis, prevention of dental caries, deodorization and detoxification, etc. In addition, tea polyphenols can also prevent the damage of sunlight to the skin.

[0019] Chlorogenic acid is one of the main antibacterial and antiviral effective pharmacological components of honeysuckle, has a wide range of biological activities, and has the effects of antibacterial, antiviral, increasing white blood cells, protecting the liver and gallbladder, anti-tumor, blood pressure-lowering, lipid-lowering, scavenging free radicals and exciting the central nervous system, etc.

[0020] Menthol is the main component in peppermint and European peppermint essential oils, and is used as a stimulant in medicine, acting on the skin or mucous membrane, having a cooling and antipruritic effect; taken orally, it can be used as an antispasmodic for headache and inflammation of the nose, pharynx, larynx, etc.

[0021] Ornidazole is commonly used in clinical medication for the treatment of infections caused by anaerobic bacteria, protozoa, trichomonads, etc. For example, it is used to treat various infectious diseases caused by sensitive anaerobic bacteria such as Bacteroides fragilis, Bacteroides distasonis, Bacteroides ovatus, Bacteroides thetaiotaomicron, Bacteroides vulgatus, Clostridium spp., Eubacterium spp., Peptococcus spp., Peptostreptococcus spp., Helicobacter pylori, Bacteroides melaninogenicus, Fusobacterium spp., Capnocytophaga spp., and Bacteroides gingivalis. Ornidazole reduces to amino groups in an anaerobic environment through the nitro group in the molecule or forms free radicals, interacts with cell components, and thus causes the death of microorganisms.

[0022] Lincomycin belongs to the lincomycin class of antibiotics. It acts on the 50S subunit of the ribosome of sensitive bacteria, can prevent the elongation of peptide chains, and thus inhibits the protein synthesis of bacterial cells. It is commonly used for respiratory tract infections, skin and soft tissue infections, female genital tract and pelvic infections caused by Staphylococcus spp., Streptococcus pyogenes, Streptococcus pneumoniae, and anaerobic bacteria, and abdominal infections caused by anaerobic bacteria.

[0023] Mometasone furoate is a new topical, non-fluorinated but halogen-containing "potent" glucocorticoid hormone preparation. The dose that exerts local anti-inflammatory effects will not cause systemic effects, and it has the characteristics of increased action intensity without a disproportionate increase in adverse reactions. After being absorbed through the oral mucosa, mometasone furoate binds to the glucocorticoid receptor protein in the cytoplasm and exerts strong anti-inflammatory, anti-allergic, vasoconstricting, reducing vascular permeability, reducing exudation, inhibiting cell division and other effects. Moreover, it can enhance the Th1 (helper T cell 1) response and inhibit the Th2 (helper T cell 2) response, making the original Th1 / Th2 imbalance tend to be balanced. It is not likely to cause adverse reactions such as oral mucosal atrophy or adrenal cortical function inhibition; it is applicable to oral diseases effective for corticosteroid treatment.

[0024] Vitamin B2, also known as riboflavin, is a component of the coenzyme of flavin enzymes in the body (flavin enzymes play a role in hydrogen transfer in biological oxidation and reduction). When lacking, it affects the body's biological oxidation and causes metabolic disorders. Its lesions are mostly manifested as inflammations in the mouth, eyes, and external genitalia, such as angular cheilitis, cheilitis, glossitis, conjunctivitis, and balanitis.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] The pet spray of the present invention contains traditional Chinese medicine components, western medicine components, and a buffer solution, which can fully exert the synergistic effect between the traditional Chinese medicine components and the western medicine components. When used for the prevention and treatment of oral diseases, it has high effective drug utilization, good effects, and is not likely to produce drug resistance. Detailed implementation mode

[0027] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0028] The first aspect of the present invention provides a pet spray, and its raw material components include: traditional Chinese medicine components, western medicine components and a pH buffer; by weight percentage,

[0029] The traditional Chinese medicine components account for 2 to 27 parts, and it includes: 0.1 to 1 part of tea polyphenols, 1 to 15 parts of chlorogenic acid, and 0.1 to 1 part of menthol;

[0030] The western medicine components account for 1 to 15 parts, and it includes: 1 to 10 parts of ornidazole, 0.1 to 2 parts of lincomycin, 0.1 to 0.5 parts of mometasone furoate, 0.1 to 2 parts of vitamin B2;

[0031] The pH buffer accounts for 58 to 96 parts.

[0032] In the present invention, a pet spray is prepared by using traditional Chinese medicine components (tea polyphenols, chlorogenic acid, menthol) and western medicine components (ornidazole, lincomycin, mometasone furoate, vitamin B2). It can not only play the role of treatment, regulation and deodorization of traditional Chinese medicine, but also play the roles of broad-spectrum and powerful antibacterial, anti-inflammatory, anti-allergic, vasoconstriction, reducing vascular permeability, reducing exudation, inhibiting cell division, etc. of western medicine. The traditional Chinese medicine and western medicine cooperate with each other and enhance the curative effect in protecting the gums and eliminating bad breath, and restrict each other in the immunosuppression of oral mucosa. Local diseases are treated locally, reducing the toxic and side effects, and it can be effectively used for the treatment and prevention of pet oral diseases and is not easy to produce drug resistance. In this embodiment, preferably, the traditional Chinese medicine components account for 6 to 27 parts, the western medicine components account for 1.5 to 14 parts, and the pH buffer accounts for 61 to 87 parts. Further, the mass ratio of the traditional Chinese medicine components to the western medicine components is (0.4 to 4.5):1. Within this range, the synergistic effect between the traditional Chinese medicine components and the western medicine components can be more effectively exerted, and the obtained curative effect is better. More preferably, it is (0.4 to 2.5):1.

[0033] In the present invention, the compounding of chlorogenic acid and menthol can enable the two to act synergistically on the cell membrane of aerobic bacteria, changing the normal pH value inside and outside the cell, so as to achieve the purpose of killing aerobic bacteria. In this embodiment, the mass ratio of chlorogenic acid to menthol is 1:(0.01 to 0.3). Within this ratio range, the synergistic effect of the two can be better exerted. Preferably, it is 1:(0.02 to 0.03), and more preferably, it is 1:0.03.

[0034] In the present invention, the role of the pH buffer solution is to control the pH of the pet spray to 6.5 - 7.5. If the pH is too high or too low, it will cause certain irritation to tooth enamel and gums; too high pH may also promote the formation of dental calculus; too low pH can inhibit the growth of normal flora such as Streptococcus salivarius in the oral cavity, promote the reproduction of acid-producing bacteria and harmful bacteria, and exacerbate dental caries. The present invention does not particularly limit the specific type of the buffer solution, and those skilled in the art can select the corresponding types commonly used in the art as long as they can play the corresponding role. For example, the selected buffer solution can be phosphate buffer solution and / or carbonate buffer solution. In some specific embodiments of the present invention, the pH buffer solution is phosphate buffer solution, and the phosphate buffer solution is composed of disodium hydrogen phosphate, sodium dihydrogen phosphate and water. Further, in the phosphate buffer solution, the mass fraction of disodium hydrogen phosphate is 0.454 - 1.945 wt%, and the mass fraction of sodium dihydrogen phosphate is 0.756 - 2.016%. At room temperature, the pH value of the phosphate buffer solution is 6.5 - 7.5.

[0035] The second aspect of the present invention provides a preparation method of a pet spray, including the following steps: proportioning the raw materials according to the above ratios, mixing them evenly to obtain a solution-type pet spray.

[0036] The preparation method of the pet spray provided in the second aspect of the present invention is used to obtain the pet spray provided in the first aspect of the present invention.

[0037] To avoid repetition, in the following examples and comparative examples of the present invention, the composition of the selected phosphate buffer solution is: each 100 g of the phosphate buffer solution contains 0.454 - 1.945 g of disodium hydrogen phosphate, 0.756 - 2.016 g of sodium dihydrogen phosphate, and 96.0836 - 98 g of water. At room temperature, the pH value of the phosphate buffer solution is 6.5 - 7.5.

[0038] Example 1

[0039] This example provides a pet spray, and its raw materials include the following components:

[0040] 0.15 g of tea polyphenols, 5 g of chlorogenic acid, 0.15 g of menthol, 5 g of ornidazole, 0.6 g of lincomycin, 0.12 g of mometasone furoate, 0.5 g of vitamin B2, and 85.98 g of phosphate buffer solution.

[0041] This example also provides a preparation method of a pet spray, including the following steps: proportioning the raw materials according to the above ratios, mixing them evenly, and then packaging them into spray bottles to obtain a solution-type pet spray.

[0042] The differences between Examples 2 - 7 and Comparative Examples 1 - 7 and Example 1 are only that the raw material compositions are different. First, summarize the raw material compositions of Examples 1 - 7 and Comparative Examples 1 - 7 in Tables 1 and 2.

[0043] Table 1

[0044]

[0045] Table 2

[0046]

[0047] Test Example 1

[0048] Animal experiments were carried out using Examples 1-7 of the present invention, Comparative Examples 1-7 and 0.5% metronidazole solution.

[0049] A total of 3,255 diseased dogs with halitosis and stomatitis (blisters, gingivitis, glossitis) in 12 small animal protection bases were randomly divided into 15 groups, with 217 in each group. The 15 groups of diseased animals were treated with oral spray using Examples 1-7 of the present invention, Comparative Examples 1-7 and 0.5% metronidazole solution respectively. All diseased dogs were treated once in the morning and once in the evening every day. The treatment effect was observed after 14 days. The test results are shown in Table 3.

[0050] Judgment criteria:

[0051] Cure means that the diseased dog has normal eating and chewing movements, no pain (no salivation), the ulcer surface disappears, the swelling subsides, and there is no special odor (halitosis) in the oral cavity;

[0052] Effective means that the eating and chewing movements of the diseased dog are improved or normal, the pain is slight or disappears (salivation decreases), the ulcer surface decreases or disappears, the swelling is reduced or subsides, and the special odor (halitosis) in the oral cavity is weakened or disappears.

[0053] Table 3

[0054]

[0055]

[0056] It can be seen from Table 3 that the cure rate of Examples 1-7 of the present invention for treating pet oral diseases reaches more than 93%, and the effective rate reaches more than 98%, which is significantly better than the control group using only 0.5% metronidazole solution, indicating that Examples 1-7 of the present invention have good treatment effects on treating pet oral diseases. Compared with Example 1, the only difference between Example 6 and Example 7 lies in the mass ratio of chlorogenic acid and menthol, indicating that the mass ratio of chlorogenic acid and menthol has an important influence on the treatment effect of pet oral diseases, and both too high and too low are not conducive to improving the treatment effect.

[0057] Compared with Example 1, in Comparative Example 1, fewer traditional Chinese medicine components and more western medicine components were added. In Comparative Example 2, more traditional Chinese medicine components and fewer western medicine components were added. The cure rate and effective rate for pet oral diseases were both poor, indicating that the contents of the traditional Chinese medicine components and western medicine components in this formula need to be strictly controlled within a certain range. Too much or too little is not conducive to giving full play to the synergistic effect of the two.

[0058] Compared with Example 1, ornidazole, tea polyphenols, lincomycin, mometasone furoate, and chlorogenic acid were not added in Comparative Examples 3 to 7 respectively, indicating that only when the contents of the traditional Chinese medicine components and western medicine components of the present invention are limited within the scope of the present invention can the synergistic effect of each component be fully exerted, and a spray with good therapeutic effect for treating pet oral diseases can be obtained; the lack of any component will cause it to be unable to play a synergistic effect with other components, which is not conducive to improving the therapeutic effect.

[0059] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0060] The pet spray of the present invention contains traditional Chinese medicine components, western medicine components, and a buffer solution, which can fully exert the synergistic effect between the traditional Chinese medicine components and western medicine components. When it is used for the prevention and treatment of oral diseases, the effective drug utilization rate is high, the effect is good, and drug resistance is not easily generated;

[0061] The pet spray of the present invention can be used for the treatment and prevention of pet oral diseases such as oral ulcers, gingivitis, periodontitis, glossitis, dental calculus, and bad breath, and has the characteristics of oral deodorization, antibacterial, anti-inflammatory, anti-allergic, local treatment of local diseases, low production cost, good curative effect, high safety, and good economic benefits.

[0062] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered by the protection scope of the present invention.

Claims

1. A spray for pets, characterized in that, Its raw material components include: traditional Chinese medicine components, western medicine components and a pH buffer; by weight percentage, the traditional Chinese medicine components account for 2-27 parts, which are composed of 0.1-1 part of tea polyphenols, 1-15 parts of chlorogenic acid and 0.1-1 part of menthol; the mass ratio of chlorogenic acid to menthol is 1:(0.02-0.03); the western medicine components account for 1-15 parts, which are composed of 1-10 parts of ornidazole, 0.1-2 parts of lincomycin, 0.1-0.5 parts of mometasone furoate, and 0.1-2 parts of vitamin B2; the pH buffer accounts for 58-96 parts.

2. The pet spray according to claim 1, characterized in that, The traditional Chinese medicine components account for 6-27 parts, the western medicine components account for 1.5-14 parts, and the pH buffer accounts for 61-87 parts.

3. The pet spray according to claim 1, wherein The mass ratio of the traditional Chinese medicine components to the western medicine components is (0.4-4.5):

1.

4. The pet spray according to claim 1, characterized in that, The pH of the pet spray is controlled at 6.5-7.

5.

5. The pet spray according to claim 1, wherein The pH buffer is a phosphate buffer and / or a carbonate buffer.

6. The pet spray according to claim 5, wherein In the phosphate buffer, the mass fraction of disodium hydrogen phosphate is 0.454-1.945 wt%, and the mass fraction of sodium dihydrogen phosphate is 0.756-2.016 wt%.

7. A method for preparing the pet spray as described in any one of claims 1 to 6, characterized in that, It includes the following steps: proportion the raw materials according to the above ratios, mix them evenly to obtain a solution-type spray for pets.

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