Traditional Chinese medicine tincture for preventing and treating poliosis and extraction and preparation method thereof
A multi-targeted herbal tincture with vitamin B7, snow lotus extract, and nanoliposomes addresses the limitations of current white hair treatments by promoting melanin synthesis and scalp health, achieving superior efficacy in reducing white hair and enhancing melanin density.
Patent Information
- Application Number
- CN202510584545.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-07
- Publication Date
- 2025-07-15
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing methods of preventing and treating gray hair have problems such as chemical hair dyes causing allergies, low bioavailability, single mechanism of action, and poor transdermal absorption of active ingredients, which are difficult to meet the needs of safe and efficient gray hair treatment.
Traditional Chinese medicinal materials such as Polygonum multiflorum combined with vitamin B7, Centella Astragalus extract, probiotic fermentation filtrate and astaxanthin-nanoliposomes are used to prepare traditional Chinese medicine tinctures through the synergistic action of multiple targets, promote the function of hair follicle melanocytes, regulate the scalp microecology, and inhibit white blood generation.
Significantly improves the tyrosinase activity of hair follicles melanocytes, increases melanin content, reduces the amount of gray hair, increases transdermal absorption, and ensures safety. It is suitable for a variety of scalp types.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of traditional Chinese medicine preparation, and particularly relates to a traditional Chinese medicine tincture for preventing and treating white hair and an extraction and preparation method thereof. Background Art
[0002] The occurrence of white hair is related to multiple factors such as the decline of hair follicle melanocyte function, scalp microecological imbalance, and nutritional metabolism disorders. There are limitations in current means for preventing and treating white hair. I will elaborate on the necessity of developing this traditional Chinese medicine tincture around the existing technical problems.
[0003] With the acceleration of the pace of life and the aggravation of population aging, the problem of white hair has received increasing attention. At present, the means for preventing and treating white hair mainly include chemical hair dyes, oral nutritional supplements, and traditional Chinese medicine preparations. Although chemical hair dyes can quickly change hair color, they contain chemical components such as p-phenylenediamine, which are likely to cause adverse reactions such as allergies and scalp irritation, and may also damage hair follicle health after long-term use; oral nutritional supplements (such as vitamins and minerals) need to be absorbed through the digestive system, with problems of low bioavailability and slow onset of action, and it is difficult for a single component to solve the causes of white hair from multiple targets; traditional Chinese medicine preparations mainly consist of single herbs or simple prescriptions such as raw fleece-flower root and black sesame, and improve white hair by tonifying the liver and kidney and enriching essence and blood, but lack the consideration of modern biological mechanisms such as the regulation of the hair follicle microenvironment and the balance of scalp flora, with limited treatment effects and insufficient stability.
[0004] In addition, existing products for preventing and treating white hair generally have problems such as poor transdermal absorption of active ingredients and single action mechanisms, and it is difficult to meet the clinical needs for a safe and highly effective treatment plan for white hair. Therefore, there is an urgent need to develop a product for preventing and treating white hair based on the synergistic action of multiple targets, combining the safety of traditional Chinese medicine and the advantages of modern biotechnology. Summary of the Invention
[0005] In view of this, the present invention provides a traditional Chinese medicine tincture for preventing and treating white hair and an extraction and preparation method thereof to solve the above problems.
[0006] The technical solution of the present invention is realized as follows: A traditional Chinese medicine tincture for preventing and treating white hair is prepared from the following raw materials: by weight, 180 - 220 parts of raw fleece-flower root, 30 - 50 parts of Eclipta prostrata, 20 - 40 parts of raw madder, 3 - 7 parts of Ligusticum sinense, 3 - 7 parts of Schizonepeta tenuifolia, 12 - 18 parts of Sophora japonica flower, 10 - 20 parts of Curcuma aromatica, 15 - 25 parts of Angelica dahurica, and 0.1 - 0.5 part of vitamin B7.
[0007] Further, a traditional Chinese medicine tincture for preventing and treating white hair is prepared from the following raw materials: by weight, 200 parts of raw fleece-flower root, 40 parts of Eclipta prostrata, 30 parts of raw madder, 5 parts of Ligusticum sinense, 5 parts of Schizonepeta tenuifolia, 15 parts of Sophora japonica flower, 15 parts of Curcuma aromatica, 20 parts of Angelica dahurica, and 0.3 part of vitamin B7 Furthermore, a traditional Chinese medicine tincture for preventing and treating gray hair also includes, by weight, 2-5 parts of niacinamide, 1-3 parts of Centella asiatica extract, 5-10 parts of probiotic fermentation filtrate, and 0.01-0.1 parts of hair follicle activation factor.
[0008] Furthermore, the Centella asiatica extract is prepared by mixing 60%-80% ethanol with the Centella asiatica material and ethanol at a weight-to-volume ratio of 1:(8-12) and stirring evenly; sealing the container, soaking at room temperature for 24-48 hours, and then heating and refluxing at 60-80°C for extraction 2-3 times, each time for 1-2 hours; filtering with gauze or filter paper or centrifuging at 3000-5000rpm for 10-15 minutes for solid-liquid separation; and concentrating the filtrate by reduced pressure evaporation at -0.06 to -0.08MPa and 50-60°C to obtain the Centella asiatica extract.
[0009] Furthermore, the probiotic fermentation filtrate is prepared by inoculating bifidobacteria at a rate of 2-5% (v / v) into a culture medium containing 15-25 g / L glucose and 10-15 g / L peptone, and fermenting at 35-37°C for 24-48 hours. When the pH value drops to 4.5-5.0 and the bacterial concentration reaches 1×10 8 -1×10 9 cfu / mL, it was regarded as the fermentation end point, and the fermentation broth was centrifuged to separate the bacterial precipitate and the supernatant, the supernatant was collected, and the collected supernatant was filtered through a 0.22 μm microporous filter membrane to obtain the probiotic fermentation filtrate after sterilization.
[0010] Furthermore, the hair follicle activation factor is astaxanthin-nano liposome, which is prepared by the following method: a) mixing soybean lecithin and cholesterol in a mass ratio of 3-5:1, dissolving in chloroform, wherein the mass volume ratio of total lipid to chloroform is 1:10-20 g / mL, and rotary evaporating to form a lipid film; b) Astaxanthin was simultaneously loaded during ultrasonic hydration with pH 7.4 PBS buffer for 30-60 min, and drug-loaded liposomes with a particle size of 80-120 nm were obtained by high-pressure homogenization.
[0011] Furthermore, the stirring rate of step b) is 300-500 rpm, the ultrasonic power is 100-300 W, the frequency is 20-25 kHz, and the intermittent ultrasonication is performed for 2 seconds and then paused for 1 second.
[0012] Furthermore, a method for preparing a traditional Chinese medicine tincture for preventing and treating gray hair comprises the following steps: S1. Preparation of the medicinal material extract powder: Radix Polygoni Multiflori, Herba Ecliptae, Radix Rubiae, Rhizoma Ligustici, Herba Schizonepetae, Flos Sophorae Immaturus, Radix Curcumae, and Radix Angelicae Dahuricae are respectively pulverized, then mixed according to the raw material ratio, added with 6 - 8 times the amount of deionized water, heated to 80 - 100 °C for reflux extraction 2 - 3 times, each time for 1 - 2 hours, and the extraction solutions are combined; the extraction solution is concentrated under reduced pressure to a relative density of 1.1 - 1.3 (measured at 60 °C), and spray-dried to prepare the 10:1 medicinal material extract powder; S2. Preparation of the tincture: The medicinal material extract powder is mixed with alcohol, stirred until completely dissolved, diluted with deionized water, and vitamin B7, niacinamide, Centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor are added in sequence, and stirred evenly to obtain the traditional Chinese medicine tincture.
[0013] Further, the conditions for concentration under reduced pressure in step S1 are: pressure -0.06 to -0.08 MPa, temperature 50 - 60 °C; the inlet air temperature for spray drying is 180 - 200 °C, and the outlet air temperature is 80 - 90 °C.
[0014] Further, the alcohol in step S2 is medical alcohol with a volume concentration of 75% - 95%, the mass - to - volume ratio g / mL of the medicinal material extract powder to alcohol and deionized water is 31:(20 - 30):(40 - 50), the solution temperature when adding vitamin B7, niacinamide, Centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor is 20 - 30 °C, the stirring speed is 100 - 200 rpm, and after complete dissolution, it is allowed to stand for 1 - 3 hours.
[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) Multi - target synergistic mechanism: The formula of the present invention takes traditional Chinese medicinal materials such as Radix Polygoni Multiflori and Herba Ecliptae as the core, focuses on nourishing the liver and kidney, regulating qi and blood, and fundamentally improving the internal factors for the formation of white hair. At the same time, vitamin B7 is innovatively incorporated to promote keratin synthesis, niacinamide inhibits the transport of melanosomes, Centella asiatica extract promotes the production of collagen in hair follicles, probiotic fermentation filtrate regulates the scalp micro - ecological balance, and astaxanthin - nanoliposome targets and activates hair follicle cells, playing a four - dimensional synergistic system of inhibiting white hair growth, promoting melanin synthesis, optimizing the hair follicle micro - environment, and regulating the scalp flora. In vitro experimental data show that this formula can significantly increase the tyrosinase activity of hair follicle melanocytes by 153% and increase the melanin content by 176%, showing a highly significant difference compared with the traditional Chinese medicine group (P < 0.001). (2)Process effectiveness: By adopting traditional Chinese medicine concentration extraction and spray drying technologies, the extraction rate of active ingredients has been significantly improved. After Centella asiatica is extracted with ethanol and combined with vacuum concentration, the active ingredients such as asiaticoside are ensured to be retained in a high proportion. Astaxanthin is encapsulated by liposomes with the particle size controlled within 80 - 120 nm, which improves transdermal absorption and can also prevent photothermal degradation. Animal experiments show that the tincture can increase the melanin density in white-haired mice by 114% and reduce the number of white hairs by 57%, and the effect is better than that of the positive control drug (P < 0.01).
[0016] (3)High safety and strong adaptability: Using medical alcohol to promote penetration and deionized water to adjust the pH to 5.5 - 6.5, it fits the weakly acidic environment of the scalp and reduces irritation. The probiotic fermentation filtrate inhibits harmful bacteria and improves scalp inflammation. The formula does not contain hormones and heavy metals, and there is no allergic reaction in the skin irritation test. It can be used by various sensitive scalp populations, providing a safe and efficient new solution for preventing and treating white hair. Specific implementation mode
[0017] To better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.
[0018] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.
[0019] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can all be obtained from commercial channels. Embodiment
[0020] Raw material ratio By weight, 180 parts of raw Polygonum multiflorum, 30 parts of Eclipta prostrata, 20 parts of raw Rubia cordifolia, 3 parts of Ligusticum sinense, 3 parts of Schizonepeta tenuifolia, 12 parts of Sophora japonica, 10 parts of Curcuma aromatica, 15 parts of Angelica dahurica, 0.1 part of vitamin B7, 2 parts of nicotinamide, 1 part of Centella asiatica extract, 5 parts of probiotic fermentation filtrate, 0.01 part of hair follicle activation factor, 200 g of alcohol, and 400 g of deionized water. Embodiment
[0021] Raw material ratio By weight, 220 parts of raw Polygonum multiflorum, 50 parts of Eclipta prostrata, 40 parts of raw Rubia cordifolia, 7 parts of Ligusticum sinense, 7 parts of Schizonepeta tenuifolia, 18 parts of Sophora japonica, 20 parts of Curcuma aromatica, 25 parts of Angelica dahurica, 0.5 part of vitamin B7, 5 parts of nicotinamide, 3 parts of Centella asiatica extract, 10 parts of probiotic fermentation filtrate, 0.1 part of hair follicle activation factor, 300 g of alcohol, and 500 g of deionized water. Embodiment
[0022] Raw material ratio By weight, take 200 parts of raw Polygonum multiflorum, 40 parts of Eclipta prostrata, 30 parts of raw Rubia cordifolia, 5 parts of Ligusticum chuanxiong, 5 parts of Schizonepeta tenuifolia, 15 parts of Sophora japonica, 15 parts of Curcuma aromatica, 20 parts of Angelica dahurica, 70.3 parts of vitamin B, 3 parts of niacinamide, 2 parts of Centella asiatica extract, 8 parts of probiotic fermentation filtrate, 0.05 parts of hair follicle activating factor, 250 g of alcohol, and 440 g of deionized water.
[0023] The above examples 1-3 adopt the following preparation method: Preparation of Centella asiatica extract: Centella asiatica extract is prepared by mixing 70% ethanol with ethanol in a weight-to-volume ratio of 1:10 and stirring evenly; the container is sealed, and after soaking at room temperature for 36 hours, it is heated to reflux at 70°C for extraction 3 times, each time for 2 hours; centrifugation is performed at 4000rpm for 12 minutes to separate the solid and liquid; the filtrate is concentrated by reduced pressure evaporation at -0.07MPa and 55°C to obtain the Centella asiatica extract.
[0024] Preparation of probiotic fermentation filtrate: Probiotic fermentation filtrate is prepared by inoculating bifidobacteria at a 3% (v / v) inoculum into a medium containing 20 g / L glucose and 12 g / L peptone and fermenting at 36 °C for 36 hours. When the pH value drops to 4.5 and the bacterial concentration reaches 1×10 8 cfu / mL, it was regarded as the fermentation end point, and the fermentation broth was centrifuged to separate the bacterial precipitate and the supernatant, the supernatant was collected, and the collected supernatant was filtered through a 0.22 μm microporous filter membrane to obtain the probiotic fermentation filtrate after sterilization.
[0025] Preparation of hair follicle activating factor: a) mixing soybean lecithin and cholesterol in a mass ratio of 4:1, dissolving in chloroform, the mass volume ratio of total lipid to chloroform being 1:15 g / mL, and rotary evaporating to form a lipid film; b) Astaxanthin was simultaneously loaded during ultrasonic hydration with pH 7.4 PBS buffer at a stirring rate of 400 rpm, an ultrasonic power of 200 W, a frequency of 22 kHz, intermittent ultrasonication for 2 s and a pause of 1 s, a hydration time of 50 min, and drug-loaded liposomes with a particle size of 100 nm were obtained by high-pressure homogenization.
[0026] S1. Preparation of medicinal material extract powder: grind raw Polygonum multiflorum, Eclipta prostrata, raw Rubia cordifolia, Ligusticum chuanxiong, Nepeta tenuifolia, Sophora japonica, Curcuma aromatica and Angelica dahurica respectively, mix according to the raw material ratio, add 7 times amount of deionized water, reflux extraction at 90°C for 2 times, each time for 1.5 hours, combine the extracts; concentrate under reduced pressure at a pressure of -0.07MPa and a temperature of 55°C to a relative density of 1.2 (measured at 60°C), spray dry at an inlet air temperature of 190°C and an outlet air temperature of 85°C to prepare a 10:1 medicinal material extract powder; S2. Tincture Preparation: Mix 310 g of powdered medicinal herb extract with 250 mL of 75% medical alcohol and dissolve. Add 440 mL of deionized water for dilution. Then, add vitamin B7, niacinamide, centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor in sequence. Stir at 25°C at 150 rpm until completely dissolved, and then let it stand for 2 hours to obtain the traditional Chinese medicine tincture.
[0027] Raw Material Ratio: By weight, 200 parts of raw fleeceflower root, 40 parts of eclipta prostrata, 30 parts of raw madder, 5 parts of ligusticum sinense oliv, 5 parts of schizonepeta tenuifolia, 15 parts of sophora japonica flower, 15 parts of curcuma aromatica, 20 parts of angelica dahurica, 250 g of alcohol, and 440 g of deionized water.
[0028] Preparation Method Refer to the steps of preparing the powdered medicinal herb extract and the tincture in Example 3, without adding vitamin B7, niacinamide, centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor, to obtain the traditional Chinese medicine tincture.
[0029] Raw Material Ratio By weight, 200 parts of raw fleeceflower root, 40 parts of eclipta prostrata, 30 parts of raw madder, 5 parts of ligusticum sinense oliv, 5 parts of schizonepeta tenuifolia, 15 parts of sophora japonica flower, 15 parts of curcuma aromatica, 20 parts of angelica dahurica, 0.3 part of vitamin B7, 3 parts of niacinamide, 2 parts of centella asiatica extract, 8 parts of probiotic fermentation filtrate, 250 g of alcohol, and 440 g of deionized water.
[0030] Preparation Method Different from Example 3, without adding the hair follicle activation factor and with the remaining steps the same, to obtain the traditional Chinese medicine tincture without the hair follicle activation factor.
[0031] Raw Material Ratio By weight, take 200 parts of raw fleeceflower root, 40 parts of eclipta prostrata, 30 parts of raw madder, 5 parts of ligusticum sinense oliv, 5 parts of schizonepeta tenuifolia, 15 parts of sophora japonica flower, 15 parts of curcuma aromatica, 20 parts of angelica dahurica, 0.3 part of vitamin B7, 3 parts of niacinamide, 2 parts of centella asiatica extract, 0.05 part of hair follicle activation factor, 250 g of alcohol, and 440 g of deionized water.
[0032] Preparation Method Different from Example 3, without adding the probiotic fermentation filtrate and with the remaining steps the same, to obtain the traditional Chinese medicine tincture without the probiotic fermentation filtrate.
[0033] Raw Material Ratio By weight, take 200 parts of raw fleeceflower root, 40 parts of eclipta prostrata, 30 parts of raw madder, 5 parts of ligusticum sinense oliv, 5 parts of schizonepeta tenuifolia, 15 parts of sophora japonica flower, 15 parts of curcuma aromatica, 20 parts of angelica dahurica, 0.3 part of vitamin B7, 3 parts of niacinamide, 8 parts of probiotic fermentation filtrate, 0.05 part of hair follicle activation factor, 250 g of alcohol, and 440 g of deionized water.
[0034] Preparation Method Differing from Example 3, no centella asiatica extract was added, and the remaining steps were the same, resulting in a traditional Chinese medicine tincture without centella asiatica extract.
[0035] 1. Cell model: Human follicular melanocytes (HFM3), cultured to the logarithmic growth phase.
[0036] 2. Grouping and treatment: (1) Blank group: An equal amount of normal saline was added to the culture medium. (2) Example 1 - 3 groups: 1% (v / v) traditional Chinese medicine tincture was added to the culture medium. (3) Comparative example 1 - 4 groups: The corresponding tinctures (1% v / v) were added respectively. 3. Detection indicators: (1) Melanin content: After cell lysis, melanin was dissolved with NaOH, and the absorbance (OD value) was measured at a wavelength of 750 nm.
[0037] (2) Tyrosinase activity: The L - DOPA substrate method was used, and the absorbance was measured at 475 nm to calculate the enzyme activity unit (U / mL).
[0038] (3) Cell viability: Detected by the CCK - 8 method, and the cell proliferation rate (%) was calculated.
[0039] 4. Culture time: 72 hours, and the experiment was repeated 3 times.
[0040] 5. Results of in vitro follicular melanocyte experiments Note: Compared with the blank group, *P < 0.05, **P < 0.01, ***P < 0.001 (t - test).
[0041] The data showed that the example groups had significant effects in promoting melanin production, increasing tyrosinase activity, and enhancing cell viability, and were superior to each comparative example group.
[0042] 1. Animal model: BALB / c white mice (8 - week - old, male), and a white hair model was induced by topical application of hydroquinone cream on the back skin.
[0043] 2. Grouping and treatment (n = 10 / group): Blank group: Topical application of normal saline. Positive control group: Topical application of 2% minoxidil tincture. Example 1 - 3 groups: Topical application of the prepared traditional Chinese medicine tincture. Comparative example 1 - 4 groups: Topical application of the corresponding tinctures. 3. Usage method: Apply twice a day to the modeled area, massage for 3 minutes, and continuously treat for 21 days.
[0044] 4. Detection indicators: (1)Hair melanin density: Optical microscopy was used for photographing, and the melanin granule density (pixel value / μm²) was analyzed by ImageJ software.
[0045] (2)Change in the number of white hairs: The number of white hairs with a diameter > 50 μm was counted (number of hairs / cm²).
[0046] (3)Number of melanocytes in hair follicles: Skin tissue sections were subjected to immunohistochemical staining with tyrosinase-related protein-1 (TRP-1), and the number of positive cells in the hair follicle unit was counted.
[0047] 5. In-vivo experimental results of the white hair model mice Note: Compared with the blank group, *P < 0.05, **P < 0.01, ***P < 0.001 (t-test).
[0048] Data description Comparison among groups of Examples 1 - 3: In Example 3, due to the use of the optimized ratio (such as 2 parts of Centella asiatica extract and 8 parts of probiotic fermentation filtrate), the melanin density and the number of follicular cells were slightly higher than those in Examples 1 and 2. However, all three showed extremely significant differences (***), proving that the formula is effective within a relatively wide range of raw materials.
[0049] Differences between the comparative examples and the examples: Comparative Example 1 (traditional formula) only took effect through basic traditional Chinese medicine components, and its effect was weaker than that of all examples, verifying the necessity of modern active ingredients (such as vitamin B7, niacinamide, etc.).
[0050] The effects of Comparative Examples 2 - 4 (lacking a single component) were between those of the traditional formula and the examples, indicating that the synergistic effects of Centella asiatica extract (improving the hair follicle microenvironment), probiotic fermentation filtrate (regulating the flora), and follicular activating factor (targeting hair follicles) are irreplaceable.
[0051] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.
Claims
1. A traditional Chinese medicine tincture for preventing and treating white hair, characterized in that: It is made from the following raw materials: by weight, 180 - 220 parts of raw fleece-flower root, 30 - 50 parts of eclipta prostrata, 20 - 40 parts of raw madder, 3 - 7 parts of ligusticum sinense, 3 - 7 parts of schizonepeta tenuifolia, 12 - 18 parts of sophora japonica flower, 10 - 20 parts of turmeric root-tuber, 15 - 25 parts of dahurian angelica root, and 0.1 - 0.5 part of vitamin B7.
2. The traditional Chinese medicine tincture for preventing and treating white hair according to claim 1, wherein: It is made from the following raw materials: by weight, 200 parts of raw fleece-flower root, 40 parts of eclipta prostrata, 30 parts of raw madder, 5 parts of ligusticum sinense, 5 parts of schizonepeta tenuifolia, 15 parts of sophora japonica flower, 15 parts of turmeric root-tuber, 20 parts of dahurian angelica root, and 0.3 part of vitamin B7.
3. A traditional Chinese medicine tincture for preventing and treating white hair as claimed in claim 1 or 2, characterized in that: It also includes 2 - 5 parts of niacinamide, 1 - 3 parts of centella asiatica extract, 5 - 10 parts of probiotic fermentation filtrate, and 0.01 - 0.1 part of hair follicle activation factor.
4. The traditional Chinese medicine tincture for preventing and treating white hair according to claim 3, characterized in that: The centella asiatica extract is prepared by selecting ethanol with a concentration of 60% - 80%, mixing the centella asiatica medicinal material and ethanol at a weight - to - volume ratio of 1:(8 - 12), and stirring evenly; sealing the container, soaking at room temperature for 24 - 48 hours, then heating and refluxing at 60 - 80°C for 2 - 3 times, each time for 1 - 2 hours; filtering with gauze or filter paper, or centrifuging at 3000 - 5000 rpm for 10 - 15 minutes for solid - liquid separation; concentrating the filtrate by vacuum evaporation at - 0.06 to - 0.08 MPa and 50 - 60°C to obtain the centella asiatica extract.
5. The Chinese medicine tincture for preventing and treating white hair according to claim 3, characterized in that: The probiotic fermentation filtrate is prepared by inoculating Bifidobacterium into a medium containing 15 - 25 g / L glucose and 10 - 15 g / L peptone at an inoculum size of 2 - 5% (v / v), fermenting at 35 - 37 °C for 24 - 48 hours. When the pH value drops to 4.5 - 5.0 and the bacterial concentration reaches 1×10 8 -1×10 9 cfu / mL, it is regarded as the fermentation end point. The fermentation broth is centrifuged to separate the bacterial cell precipitate and the supernatant. The supernatant is collected and filtered through a 0.22 μm microporous membrane to obtain the probiotic fermentation filtrate after sterilization.
6. The Chinese medicine tincture for preventing and treating white hair according to claim 3, characterized in that: The hair follicle activation factor is astaxanthin - nanoliposome, which is prepared by the following method: Mix soy lecithin and cholesterol at a mass ratio of 3 - 5:1, dissolve them in chloroform, and the mass - to - volume ratio of the total lipid to chloroform is 1:10 - 20 g / mL. Rotate and evaporate to form a lipid film. Synchronously load astaxanthin when stirring and ultrasonically hydrating with pH 7.4 PBS buffer solution, with a hydration time of 30 - 60 min, and obtain drug - loaded liposomes with a particle size of 80 - 120 nm through high - pressure homogenization.
7. The traditional Chinese medicine tincture for preventing and treating white hair according to claim 6, wherein: The stirring rate in step b) is 300 - 500 rpm, the ultrasonic power is 100 - 300 W, the frequency is 20 - 25 kHz, and the intermittent ultrasonic wave pauses for 1 s every 2 s.
8. The preparation method of a traditional Chinese medicine tincture for preventing and treating white hair as described in claim 1, characterized in that: It includes the following steps: S1. Preparation of powdered medicinal material extract: After separately pulverizing raw fleece-flower root, eclipta prostrata, raw madder, ligusticum sinense, schizonepeta tenuifolia, sophora japonica flower, turmeric root-tuber, and dahurian angelica root, mix them according to the raw material ratio, add 6 - 8 times the amount of deionized water, heat to 80 - 100°C and reflux for 2 - 3 times, each time for 1 - 2 hours, and combine the extracts; concentrate the extract under reduced pressure to a relative density of 1.1 - 1.3 (measured at 60°C), and spray - dry to make a 10:1 powdered medicinal material extract. S2. Preparation of tincture: Mix the powdered medicinal material extract with alcohol, stir until completely dissolved, add deionized water for dilution, and then add vitamin B7, niacinamide, centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor in sequence, and stir evenly to obtain the traditional Chinese medicine tincture.
9. The preparation method of a traditional Chinese medicine tincture for preventing and treating white hair according to claim 8, characterized in that: The reduced - pressure concentration conditions in step S1 are: pressure - 0.06 to - 0.08 MPa, temperature 50 - 60°C; the inlet air temperature for spray - drying is 180 - 200°C, and the outlet air temperature is 80 - 90°C.
10. The preparation method of a traditional Chinese medicine tincture for preventing and treating white hair as described in claim 8, characterized in that: The alcohol described in step S2 is medical alcohol with a volume concentration of 75%-95%. The mass-volume ratio g / mL of the medicinal material extract powder to alcohol and deionized water is 31:(20-30):(40-50). When adding vitamin B7, niacinamide, centella asiatica extract, probiotic fermentation filtrate, and hair follicle activation factor, the solution temperature is 20-30°C, and the stirring speed is 100-200 rpm. After complete dissolution, let it stand for 1-3 hours.