Preparation method and application of edible fungus polysaccharide capable of effectively promoting proliferation of paracteroides

Abstract

The invention discloses a preparation method and application of edible fungus polysaccharide capable of effectively promoting proliferation of Parabacteroides in human intestinal tracts, and belongs to the technical field of extraction of edible fungus polysaccharide. The method comprises the following steps: carrying out enzymolysis on Schizophyllumcommuneh sporocarp through a compound enzyme, then carrying out ultrasonic-assisted extraction on enzymatic hydrolysate, carrying out evaporation concentration on the obtained extract, then carrying out Co 60 gamma-irradiation treatment, further carrying out enzymolysis through papain after irradiation is completed, repeatedly freezing and thawing the enzymatic hydrolysate for 2-3 times, then carrying out ethanol precipitation, and drying to obtain the Schizophyllumcommuneh polysaccharide. The Schizophyllumcommuneh polysaccharide prepared by the method is small in molecular weight and high in solubility, can remarkably promote proliferation of paracterobacter, can be used as an ingredient of functional food, and is of great significance to development of functional products for directional regulation and control of intestinal flora.

Description

Technical Field

[0001] The present invention belongs to the technical field of edible mushroom polysaccharide extraction. Specifically, it relates to a preparation method and application of an edible mushroom polysaccharide that can effectively promote the proliferation of Parabacteroides in the human intestine. Background Art

[0002] Parabacteroides is a core member of the human gut microbiota, with a detection rate greater than 90% in the human gut sample database. This bacterium has relatively many polysaccharide utilization loci (PULs), which endow the genus Parabacteroides with a wide range of carbohydrate utilization capabilities. Studies have found that this bacterium can effectively regulate the prevention of diseases such as obesity, inflammatory bowel disease, non-alcoholic fatty liver disease, and metabolic syndrome in the human body. Therefore, Parabacteroides may be the next generation of high-quality probiotics. Currently, multiple patents such as CN118406582A, CN115612629A, and CN117660229A have disclosed the application of the genus Parabacteroides in the prevention and treatment of metabolic diseases. However, this bacterium not only has a low content in the human intestine but also has the disadvantage of difficult colonization. There is an urgent need to develop a natural edible nutrient that can effectively promote the proliferation of Parabacteroides.

[0003] Schizophyllum commune, also known as white ginseng mushroom, is a traditional edible and medicinal mushroom in China. It is deeply loved by people because of its unique flavor, high nutritional value, and containing a variety of bioactive substances. According to ancient records, Schizophyllum commune "has a neutral nature, a sweet taste, is slightly cold, non-toxic, and has the effects of clearing the liver and improving eyesight, and tonifying the body". Modern research has found that Schizophyllum commune contains a variety of bioactive substances such as polysaccharides, polyphenols, and ergosterol. Previously, Schizophyllum commune mainly came from wild picking, with limited production, and it was extremely difficult to extract polysaccharides from the fruiting body. Therefore, white ginseng mushroom polysaccharides mainly came from submerged fermentation of mycelia. In recent years, with the maturity of the artificial cultivation technology of Schizophyllum commune in China, a large number of artificial cultivation bases of Schizophyllum commune have emerged in Yunnan, Sichuan, Hubei and other places. Therefore, it has become easier to obtain polysaccharides from the fruiting body of Schizophyllum commune. In 2022, the applicant applied for the patent CN114957507B, which disclosed a composite modification method and application for enhancing the biological activity of white ginseng mushroom polysaccharides; in 2024, the applicant applied for the patent CN118165138A, which disclosed a preparation method of white ginseng mushroom glucomannan and its anti-tumor effect. However, these patents did not disclose that white ginseng mushroom polysaccharides have the biological activity of promoting the proliferation of Parabacteroides. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the first object of the present invention is to provide a preparation method of white ginseng mushroom polysaccharides that can effectively promote the large-scale proliferation of Parabacteroides.

[0005] To achieve the above technical objectives, the inventor combined years of experience in the extraction and separation of natural products, and through a large number of experimental studies and unremitting explorations, finally obtained the following technical solution: A method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides, the method comprising the following steps:

[0006] (1) Dry the fruiting bodies of Schizophyllum commune, crush them and sieve them. Soak the powdered material in water. After sufficient water absorption and swelling, adjust the pH value to 6.0 - 7.0, add a composite enzyme for enzymatic hydrolysis. The dosage of the enzyme is 100,000 - 300,000 U of papain, 80,000 - 150,000 U of cellulase, and 30,000 - 60,000 U of pectinase per kilogram of the powdered material. Carry out enzymatic hydrolysis at 30 - 37°C for 3 - 5 h; Subsequently, carry out ultrasonic-assisted extraction on the enzymatic hydrolysate, with an ultrasonic power of 350 - 500 W and an ultrasonic time of 5 - 20 min. Separate the supernatant, add water and repeat the extraction. After a total of 2 - 4 extractions, combine the supernatants and carry out evaporation and concentration treatment;

[0007] (2) Take the concentrated solution, fill it into a PET food plastic bottle, and carry out Co 60 γ-irradiation treatment, with an irradiation dose of 5 - 30 kGy and an irradiation dose rate of 0.1 - 0.5 kGy / min; After irradiation, add 200,000 - 800,000 U of papain per liter of the concentrated solution, and carry out enzymatic hydrolysis at 30 - 37°C for 1 - 2 h; After enzymatic hydrolysis, freeze-thaw the enzymatic hydrolysate 2 - 3 times, and then precipitate the enzymatic hydrolysate with ethanol and dry it to obtain the polysaccharide from Schizophyllum commune.

[0008] Further preferably, in the method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides as described above, in step (1), the fruiting bodies of Schizophyllum commune are dried, crushed and sieved through a 60 - 120 mesh sieve after drying.

[0009] Further preferably, in the method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides as described above, in step (1), the powdered material is soaked in 20 - 50 times the amount of water.

[0010] Further preferably, in the method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides as described above, in step (1), the dosage of the enzyme is 180,000 - 230,000 U of papain, 80,000 - 120,000 U of cellulase, and 40,000 - 50,000 U of pectinase per kilogram of the powdered material.

[0011] Further preferably, in the method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides as described above, in step (1), the concentration ratio of the evaporation and concentration treatment is 10:1 - 15:1.

[0012] Further preferably, in the method for preparing polysaccharide from Schizophyllum commune that promotes the proliferation of Parabacteroides as described above, in step (2), the irradiation dose is 15 - 25 kGy.

[0013] Further preferably, for the method for preparing the polysaccharide of Schizophyllum commune promoting the proliferation of Parabacteroides as described above, in step (2), the dosage of the enzyme during enzymatic hydrolysis is 400,000 - 600,000 U of papain added per liter of the concentrated solution.

[0014] Further preferably, for the method for preparing the polysaccharide of Schizophyllum commune promoting the proliferation of Parabacteroides as described above, when the concentrated solution is precipitated with ethanol in step (2), the volume ratio of the concentrated solution to ethanol is (3 - 5):1.

[0015] In addition, the inventors of the present invention confirmed through in vitro anaerobic fermentation experiments of human feces that the polysaccharide of Schizophyllum commune prepared by the above method can effectively promote the proliferation of Parabacteroides. It can be seen that the polysaccharide of Schizophyllum commune of the present invention can be used as a functional food ingredient to promote the proliferation of Parabacteroides in the intestine.

[0016] Therefore, the second object of the present invention is to provide the application of the polysaccharide of Schizophyllum commune prepared by the above method in promoting the proliferation of Parabacteroides; and the application of the polysaccharide of Schizophyllum commune prepared by the above method in the preparation of drugs, health products or functional foods that promote the proliferation of Parabacteroides in the human intestine.

[0017] Compared with the prior art, the polysaccharide of Schizophyllum commune prepared by the present invention has a small molecular weight, high solubility, and the following beneficial effects: the polysaccharide of Schizophyllum commune prepared by the present invention has a better effect on promoting the proliferation of Parabacteroides than the commercially available prebiotic fructooligosaccharide (Solarbio Co., CAS: 308066 - 66 - 2). When the addition amount is 2 g / L, the relative abundance of Parabacteroides reaches more than 20%, and compared with fructooligosaccharide, the relative abundance of Parabacteroides in the polysaccharide of Schizophyllum commune group is increased by more than 20 times.

[0018] In summary, the polysaccharide of Schizophyllum commune prepared by the present invention has a small molecular weight, high solubility, and can significantly promote the proliferation of Parabacteroides, and can be used as an ingredient of functional foods, which is of great significance for the development of products for directional regulation of intestinal flora function. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Analysis of the solubility results of the polysaccharide of Schizophyllum commune prepared in different examples;

[0020] Figure 2 Promotion of the proliferation of Parabacteroides by the polysaccharide of Schizophyllum commune prepared in different examples. DETAILED DESCRIPTION OF THE INVENTION

[0021] The present invention will be further described below through preferred embodiments. However, the scope of the present invention is not limited to the following embodiments. Any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0022] Example 1: Preparation of the polysaccharide of Schizophyllum commune

[0023] This example provides a polysaccharide from Schizophyllum commune During its preparation process, no irradiation treatment is carried out, The method is as follows:

[0024] Dry the fruiting bodies of Schizophyllum commune, crush them and sieve (80 meshes). Add 1000 g of the powdered material to 40 times the amount of water and soak. After sufficient water absorption and swelling, adjust the pH value to 7.0, add papain (200,000 U), cellulase (100,000 U) and pectinase (50,000 U), and enzymatically hydrolyze at 37 °C for 3.5 h. Use an ultrasonic-assisted method to extract the polysaccharide from Schizophyllum commune, where the ultrasonic power is 350 W and the ultrasonic time is 15 min. Centrifuge to obtain the supernatant, repeat the extraction of the filter residue with water, combine the supernatants after extraction three times and perform evaporation and concentration, with a concentration ratio of 12:1. Take 1 L of the concentrated solution and add papain (500,000 U), and enzymatically hydrolyze at 37 °C for 1 h. After completion, freeze-thaw the enzymatically hydrolyzed solution 2 times. Subsequently, precipitate the enzymatically hydrolyzed solution with ethanol in a ratio of 4:1 and dry it to obtain the polysaccharide from Schizophyllum commune.

[0025] Example 2: Preparation of polysaccharide from Schizophyllum commune

[0026] The composite modified polysaccharide from Schizophyllum commune prepared according to the method of Example 1 in Patent CN114957507B

[0027] Example 3: Preparation of polysaccharide from Schizophyllum commune

[0028] This example provides a polysaccharide from Schizophyllum commune During its preparation process, the irradiation dose is relatively low, The method is as follows:

[0029] Dry the fruiting bodies of Schizophyllum commune, crush them and sieve (80 meshes). Add 1000 g of the powdered material to 40 times the amount of water and soak. After sufficient water absorption and swelling, adjust the pH value to 7.0, add papain (200,000 U), cellulase (100,000 U) and pectinase (50,000 U), and enzymatically hydrolyze at 37 °C for 3.5 h. Use an ultrasonic-assisted method to extract the polysaccharide from Schizophyllum commune, where the ultrasonic power is 350 W and the ultrasonic time is 15 min. Centrifuge to obtain the supernatant, repeat the extraction of the filter residue with water, combine the supernatants after extraction three times and perform evaporation and concentration, with a concentration ratio of 12:1. Take an appropriate amount of the concentrated solution, put it into a PET food plastic bottle, and perform Co 60 γ-irradiation treatment, with an irradiation dose of 5 kGy and an irradiation dose rate of 0.1 kGy / min. After irradiation, take 1 L of the concentrated solution, add papain (500,000 U), and enzymatically hydrolyze at 37 °C for 1 h. After completion, freeze-thaw the enzymatically hydrolyzed solution 2 times. Subsequently, precipitate the enzymatically hydrolyzed solution with ethanol in a ratio of 4:1 and dry it to obtain the polysaccharide from Schizophyllum commune.

[0030] Example 4: Preparation of polysaccharide from Schizophyllum commune

[0031] This example provides a polysaccharide from Schizophyllum commune After the irradiation ends, no papain is added for the second enzymatic hydrolysis, nor is it subjected to repeated freezing and thawing treatment, The method is as follows:

[0032] Dry the fruiting body of Tremella fuciformis Berk. var. candidans, crush it and sieve it (80 meshes). Add 1000 g of the powdered material to 40 times the amount of water and soak it. After sufficient water absorption and swelling, adjust the pH value to 7.0. Add papain (200,000 U), cellulase (100,000 U) and pectinase (50,000 U), and carry out enzymatic hydrolysis at 37 °C for 3.5 h. The polysaccharide of Tremella fuciformis Berk. var. candidans is extracted by an ultrasonic-assisted method, where the ultrasonic power is 350 W and the ultrasonic time is 15 min. Centrifuge to obtain the supernatant, add water to the residue for repeated extraction. After extracting three times, combine the supernatants and carry out evaporation and concentration, and the concentration ratio is 12:1. Take an appropriate amount of the concentrated solution, fill it into a PET food plastic bottle, and carry out Co 60 γ-irradiation treatment, with an irradiation dose of 20 kGy and an irradiation dose rate of 0.1 kGy / min. After the irradiation is completed, precipitate the enzymatic hydrolysate and ethanol in a ratio of 4:1 and then dry it to obtain the polysaccharide of Tremella fuciformis Berk. var. candidans.

[0033] Example 5: Preparation of the polysaccharide of Tremella fuciformis Berk. var. candidans

[0034] This example provides a polysaccharide of Tremella fuciformis Berk. var. candidans, During its preparation process, the irradiation dose is relatively high, The method is as follows:

[0035] Dry the fruiting body of Tremella fuciformis Berk. var. candidans, crush it and sieve it (80 meshes). Add 1000 g of the powdered material to 40 times the amount of water and soak it. After sufficient water absorption and swelling, adjust the pH value to 7.0. Add papain (200,000 U), cellulase (100,000 U) and pectinase (50,000 U), and carry out enzymatic hydrolysis at 37 °C for 3.5 h. The polysaccharide of Tremella fuciformis Berk. var. candidans is extracted by an ultrasonic-assisted method, where the ultrasonic power is 350 W and the ultrasonic time is 15 min. Centrifuge to obtain the supernatant, add water to the residue for repeated extraction. After extracting three times, combine the supernatants and carry out evaporation and concentration, and the concentration ratio is 12:1. Take an appropriate amount of the concentrated solution, fill it into a PET food plastic bottle, and carry out Co 60 γ-irradiation treatment, with an irradiation dose of 20 kGy and an irradiation dose rate of 0.1 kGy / min. After the irradiation is completed, take 1 L of the concentrated solution, add papain (500,000 U), and carry out enzymatic hydrolysis at 37 °C for 1 h. After completion, freeze-thaw the enzymatic hydrolysate 2 times. Subsequently, precipitate the enzymatic hydrolysate and ethanol in a ratio of 4:1 and then dry it to obtain the polysaccharide of Tremella fuciformis Berk. var. candidans.

[0036] Example 6: Comparison of the physical properties and biological effects of different polysaccharide products of Tremella fuciformis Berk. var. candidans

[0037] 1 Experimental method

[0038] 1.1 Molecular weight determination

[0039] Using dextrans PJT-180, PJT-10K, PJT-40K, PJT-70K, PJT-500K and PJT-2000K as standards, accurately weigh each standard and dissolve it in sodium nitrate solution (i.e., 2 mg / mL), filter it through a 0.22 μm organic membrane, inject it into a high-performance liquid chromatograph for analysis, and determine the peak time. Using the retention time as the abscissa and the logarithm of the molecular weight as the ordinate, draw a standard curve. The preparation of the polysaccharide solution to be measured is the same as that of the above standards, and after preparation, inject the sample. According to the retention time of each polysaccharide sample, calculate the molecular weight of the polysaccharide sample by referring to the standard curve. Liquid chromatography conditions: Agilent 1100 high-performance gel permeation chromatography (HPGPC); RID differential refractive index detector; chromatographic column: TSK gel G4000 PWxL (7.8 mm × 300, 10 μm); mobile phase: 0.1 M mol / L aqueous sodium nitrate solution; flow rate: 1.0 mL / min; injection volume: 10 μL; column temperature: 35 °C.

[0040] 1.2 Solubility analysis

[0041] Put 0.1 g of the sample into 50 mL of ultrapure water, stir it in a constant-temperature magnetic stirrer (60 r / min) until the polysaccharide is completely dissolved, and record the dissolution time at different temperatures (20, 40, 60, 80, 100 °C).

[0042] 1.3 In vitro fermentation test

[0043] Select the fecal samples of 4 volunteers within the standard body weight range (BMI = 18.6 - 24), dilute them with 10% DPBS to obtain 10% (W / V, 1 g / 10 mL) fecal slurry, centrifuge at 4 °C and 400 rpm for 5 min, take the supernatant, and place it on ice for later use. For the blank group, take 9 mL of fermentation culture medium and add 1 mL of fecal bacterial suspension; for the experimental group, take 9 mL of 10 mg / mL polysaccharide culture medium and add 1 mL of fecal bacterial suspension; for the positive control group, take 9 mL of 10 mg / mL fructooligosaccharide (FOS) culture medium and add 1 mL of fecal bacterial suspension. Seal each group with kraft paper and place it in an anaerobic incubator at 37 °C to start fermentation. Sampling is carried out at 0, 8, 16, 24, 32, and 48 h respectively. After centrifugation (9000 r / min, 10 min), collect the supernatant and store it at -80 °C for later use. Take the precipitate after 48 h of fermentation to determine its intestinal flora diversity.

[0044] 2 Analysis of experimental results

[0045] 2.1 Molecular weight information of polysaccharides from Tremella fuciformis Berk. prepared in different examples

[0046] The changes in the molecular weights (Mw and Mn) and polydispersity indices (Mw / Mn) of different polysaccharides are shown in Table 1. Compared with Example 1, the molecular weights of the polysaccharides in Example 2, Example 3, Example 4, and Example 5 all decreased significantly. The molecular weights of the polysaccharides from Schizophyllum commune in Example 3 and Example 4 were basically the same, with no obvious change. In addition, the molecular weights of the polysaccharides from Schizophyllum commune in Example 3, Example 4, and Example 5 were all lower than that of the polysaccharide in Example 2.

[0047] Table 1 Information on the molecular weights of the polysaccharides from Schizophyllum commune in different examples

[0048]

[0049] 2.2 Analysis of the solubility of the polysaccharides from Schizophyllum commune in different examples

[0050] Solubility is an important index for high-molecular-weight polysaccharides and affects their commercial applications. The solubilities of the polysaccharides from Schizophyllum commune in different examples are as Figure 1 shown. As the dissolution temperature increases, the dissolution times of the polysaccharides from Schizophyllum commune in different examples gradually shorten. At any given temperature, the dissolution time of the polysaccharide from Schizophyllum commune in Example 4 is the shortest, indicating that it has better solubility. Comparing Example 4 and Example 5, it can be seen that without secondary papain enzymolysis and without repeated freezing and thawing treatment, the solubility of the polysaccharide from Schizophyllum commune is significantly affected. This shows that the secondary papain enzymolysis and repeated freezing and thawing treatment of the present invention can effectively reduce the content of impurities such as proteins in the polysaccharide and effectively increase the solubility of the polysaccharide.

[0051] 2.3 Analysis of the promoting effect of the polysaccharides from Schizophyllum commune in different examples on the proliferation of Parabacteroides in the human intestine

[0052] An equal amount of human intestinal flora suspension was inoculated into a fermentation medium with the polysaccharide from Schizophyllum commune as the sole carbon source in different examples. After 48 hours of fermentation, the changes in the structure and composition of the intestinal microorganisms in the fermentation broth were analyzed by 16S rRNA high-throughput sequencing technology. The relative abundances of the flora at the genus level are as Figure 2As shown in the figure and Table 2. It can be seen from the figure that, compared with the blank control group, Example 1, Example 2, Example 3, Example 4 and Example 5 significantly promoted the proliferation of Bacteroides paracatenulatum. In particular, the relative abundance of Bacteroides paracatenulatum in Example 5 was 5.56 times that of the blank control group. As a well-recognized prebiotic, fructooligosaccharide did not have an obvious effect on the proliferation of Bacteroides paracatenulatum, and the relative abundance of Bacteroides paracatenulatum was reduced compared with the blank control group. Compared with the fructooligosaccharide group, the relative abundance of Bacteroides paracatenulatum in Example 5 increased by 40.43 times, and the relative abundances of Bacteroides paracatenulatum in Example 2 and Example 3 increased by 27.01 times and 28.43 times respectively, both of which were smaller than the increase ratio of the relative abundance of Bacteroides in Example 5. In addition, the relative abundance of Bacteroides paracatenulatum in Example 5 was 28.17%, which was significantly higher than that in Example 4 (24.13%).

[0053] Table 2 Statistical results of the relative abundance of Bacteroides paracatenulatum in different experimental groups

[0054]

[0055] In summary, the polysaccharide of Tremella fuciformis Berk prepared by the present invention can effectively promote the proliferation of Bacteroides paracatenulatum. The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications are also regarded as the protection scope of the present invention.

Claims

1. A preparation method of white-rot fungus polysaccharide for promoting the proliferation of Parabacteroides, characterized in that, The method comprises the following steps: (1) Dry the fruiting body of Schizophyllum commune, crush it and sieve it. Soak the powder in water. After fully absorbing water and swelling, adjust the pH value to 6.0 - 7.0, add a compound enzyme for enzymatic hydrolysis. The dosage of the enzyme is adding 100,000 - 300,000 U of papain, 80,000 - 150,000 U of cellulase and 30,000 - 60,000 U of pectinase per kilogram of the powder. Carry out enzymatic hydrolysis at 30 - 37 °C for 3 - 5 h; Subsequently, carry out ultrasonic-assisted extraction on the enzymatic hydrolysate, with an ultrasonic power of 350 - 500 W and an ultrasonic time of 5 - 20 min. Separate the supernatant, add water and repeat the extraction. After extracting 2 - 4 times in total, combine the supernatants and carry out evaporation and concentration treatment; (2) Take the concentrated solution, put it into a PET food plastic bottle, and carry out Co 60 γ-irradiation treatment with an irradiation dose of 5 - 30 kGy and an irradiation dose rate of 0.1 - 0.5 kGy / min; after the irradiation is completed, add 200,000 - 800,000 U of papain per liter of the concentrated solution, and carry out enzymatic hydrolysis at 30 - 37 °C for 1 - 2 h; after the enzymatic hydrolysis is completed, freeze-thaw the enzymatic hydrolysate 2 - 3 times, and then dry the enzymatic hydrolysate after ethanol precipitation to obtain white poria cocos polysaccharide.

2. The preparation method of the polysaccharide from Schizophyllum commune promoting the proliferation of Parabacteroides according to claim 1, characterized in that In step (1), the fruiting body of Schizophyllum commune is dried, crushed and sieved through a 60 - 120 mesh sieve.

3. The preparation method of the polysaccharide from Schizophyllum commune for promoting the proliferation of Parabacteroides, according to claim 1, is characterized in that In step (1), the powder is soaked in 20 - 50 times the amount of water.

4. The preparation method of the white-rot fungus polysaccharide for promoting the proliferation of Parabacteroides according to claim 1, characterized in that, In step (1), the dosage of the enzyme is adding 180,000 - 230,000 U of papain, 80,000 - 120,000 U of cellulase and 40,000 - 50,000 U of pectinase per kilogram of the powder.

5. The preparation method of the polysaccharide from Schizophyllum commune promoting the proliferation of Parabacteroides, according to claim 1, is characterized in that In step (1), the concentration ratio of the evaporation and concentration treatment is 10:1 - 15:

1.

6. The preparation method of the polysaccharide of Schizophyllum commune promoting the proliferation of Parabacteroides as described in claim 1, characterized in that, In step (2), the irradiation dose is 15 - 25 kGy.

7. The preparation method of the polysaccharide from Schizophyllum commune promoting the proliferation of Parabacteroides, according to claim 1, characterized in that, In step (2), the dosage of the enzyme during enzymatic hydrolysis is adding 400,000 - 600,000 U of papain per liter of the concentrated solution.

8. The preparation method of the polysaccharide from Schizophyllum commune for promoting the proliferation of Parabacteroides according to claim 1, wherein, When the concentrated solution in step (2) is precipitated with ethanol, the volume ratio of the concentrated solution to ethanol is (3 - 5):

1.

9. Application of the Schizophyllum commune polysaccharide prepared by the method according to any one of claims 1 - 8 in promoting the proliferation of Parabacteroides.

10. Application of the Schizophyllum commune polysaccharide prepared by the method according to any one of claims 1 - 8 in preparing a drug, health product or functional food for promoting the proliferation of Parabacteroides in the human intestine.

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