Multi-layer structure platelet-rich plasma preparation tube and extraction method

Abstract

The multi-layer structure platelet-rich plasma preparation tube comprises a vacuum blood collection tube body, a rubber plug is installed at the top end of the vacuum blood collection tube body, and a calcium gluconate solution layer is arranged at the bottom in the vacuum blood collection tube body. A separation gel layer II is arranged on the upper side of the calcium gluconate solution layer in the vacuum blood collection tube body, and a vitamin C solution layer is arranged on the upper side of the separation gel layer II in the vacuum blood collection tube body. The invention has the beneficial effects that through the design of the separation gel layer I and the separation gel layer II, the effective isolation of the vitamin C solution layer and the calcium gluconate solution layer from the ACDA solution layer before centrifugal separation is realized, chemical reaction or degradation is avoided, and a mixture of vitamin C and PRP is conveniently and quickly obtained through centrifugal operation after blood drawing, so that the blood drawing efficiency is improved. The method is used for tissue repair, regeneration and whitening, the operation process is simplified, and the product quality and safety are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of platelet-rich plasma extraction, and particularly relates to a multi-layer structured platelet-rich plasma preparation tube and an extraction method. Background Art

[0002] Platelet rich plasma (PRP) is a plasma rich in a high concentration of platelets obtained by centrifuging autologous whole blood. PRP contains various growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), and insulin-like growth factor 1 (IGF-1), etc. These growth factors can promote cell proliferation, cardiovascular formation, and collagen synthesis, thus playing a role in tissue repair and regeneration.

[0003] In the fields of medical aesthetics and tissue repair, platelet-rich plasma (PRP) is widely used because it is rich in various growth factors and has a significant effect on tissue repair and regeneration. At the same time, vitamin C also plays an important role in whitening and skin repair due to its antioxidant properties and promoting collagen synthesis. Using both simultaneously can enhance the therapeutic effect of PRP.

[0004] Traditional PRP tubes usually contain anticoagulants (such as ACDA) and a single-layer separating gel, which are only used for separating plasma from red blood cells, with a single function. Vitamin C needs to be manually added after PRP extraction, which has a risk of contamination and poor mixing uniformity. In addition, multi-step operations increase the time cost and affect the clinical application efficiency. Summary of the Invention

[0005] The purpose of the present invention is to provide a multi-layer structured platelet-rich plasma preparation tube and an extraction method to solve the above problems.

[0006] The present invention achieves the above purpose through the following technical solutions:

[0007] A multi-layer structured platelet-rich plasma preparation tube includes a vacuum blood collection tube body. A rubber stopper is installed at the top of the vacuum blood collection tube body. A calcium gluconate solution layer is provided at the bottom inside the vacuum blood collection tube body. A second separating gel layer is provided above the calcium gluconate solution layer inside the vacuum blood collection tube body. A vitamin C solution layer is provided above the second separating gel layer inside the vacuum blood collection tube body. A first separating gel layer is provided above the vitamin C solution layer inside the vacuum blood collection tube body. An ACDA solution layer is provided above the first separating gel layer.

[0008] Further, the vacuum blood collection tube body is inserted into the rubber stopper, and the vacuum blood collection tube body is of a transparent structure.

[0009] Further, the rubber stopper is made of rubber material.

[0010] Further, the calcium gluconate solution layer is separated from the vitamin C solution layer by the second separating gel layer.

[0011] Further, the vitamin C solution layer is separated from the ACDA solution layer by the first separating gel layer.

[0012] Further, the extraction method of the preparation tube for extracting platelet-rich plasma is as follows:

[0013] Step 1: Venous blood collection. Use a blood collection needle to puncture the patient's vein, and then insert the other end of the needle tube of the blood collection needle through the rubber stopper into the vacuum blood collection tube body. Under the action of the vacuum in the tube, automatically collect the required volume of blood.

[0014] Step 2: Blood shaking. Shake the vacuum blood collection tube body after blood collection repeatedly to ensure that the ACDA solution layer is evenly mixed with the collected blood.

[0015] Step 3: Centrifugal separation. Place the shaken vacuum blood collection tube body in a centrifuge and perform centrifugation under set parameters to achieve centrifugal separation of various components in the blood.

[0016] Step 4: Shake after centrifugation. The liquid in the vacuum blood collection tube after centrifugation is mainly divided into three layers. The upper layer is plasma containing vitamin C and calcium gluconate, the middle layer is the separating gel, and the lower layer is red blood cells. Since the platelets in the plasma are mainly located in the lower layer of the plasma after centrifugation, it is necessary to turn it over and shake it evenly to make the platelets evenly distributed in the plasma. At this time, platelet-rich plasma (PRP) containing vitamin C and calcium gluconate is also obtained.

[0017] Step 5: Extraction of platelet-rich plasma. After centrifugal separation, insert the needle of an external sterile syringe through the rubber stopper into the vacuum blood collection tube, and extract the platelet-rich plasma containing vitamin C and calcium gluconate after centrifugation to obtain platelet-rich plasma containing vitamin C and calcium gluconate.

[0018] Further, in Step 2, when shaking the vacuum blood collection tube body after collecting blood, it is shaken by turning it up and down.

[0019] Further, in Step 4, when turning it over and shaking it evenly, the vacuum blood collection tube body is gently turned up and down more than twenty times, so that the platelets are evenly distributed in the plasma.

[0020] The beneficial effects of the present invention are as follows:

[0021] Through the design of the first separating gel layer and the second separating gel layer, the present invention realizes the effective isolation of the vitamin C solution layer and the calcium gluconate solution layer from the ACDA solution layer before centrifugal separation, avoiding chemical reactions or degradation. After blood collection and centrifugation, a mixture of vitamin C and PRP can be obtained conveniently and quickly for tissue repair, regeneration and whitening, simplifying the operation process and improving the product quality and safety. Brief Description of the Drawings

[0022] Figure 1 It is a schematic structural diagram of a multi-layer structure platelet-rich plasma preparation tube and extraction method according to the present invention;

[0023] Figure 2 It is a flow chart of a multi-layer structure platelet-rich plasma preparation tube and extraction method according to the present invention when extracting a mixture of platelet-rich plasma and vitamin C.

[0024] The description of the reference numerals is as follows:

[0025] 1, vacuum blood collection tube body; 2, rubber stopper; 3, calcium gluconate solution layer; 4, first separating gel layer; 5, vitamin C solution layer; 6, ACDA solution layer; 7, second separating gel layer. Detailed Embodiments

[0026] A multi-layer structured platelet-rich plasma preparation tube, comprising a vacuum blood collection tube body 1, a rubber stopper 2 is installed at the top of the vacuum blood collection tube body 1, a calcium gluconate solution layer 3 is arranged at the bottom inside the vacuum blood collection tube body 1, a second separating gel layer 7 is arranged above the calcium gluconate solution layer 3 inside the vacuum blood collection tube body 1, a vitamin C solution layer 5 is arranged above the second separating gel layer 7 inside the vacuum blood collection tube body 1, a first separating gel layer 4 is arranged above the vitamin C solution layer 5 inside the vacuum blood collection tube body 1, an ACDA solution layer 6 is arranged above the first separating gel layer 4. The ACDA solution layer 6 is mainly used to prevent blood coagulation, and a sodium citrate solution can also be used instead of the ACDA solution to prevent blood coagulation. The vitamin C solution layer 5 is used for whitening, antioxidant protection and promoting collagen synthesis. The calcium gluconate solution layer 3 is used for PRP activation to release growth factors, and a calcium chloride solution can also be used instead of the calcium gluconate solution for PRP activation to release growth factors. The designs of the first separating gel layer 4 and the second separating gel layer 7 achieve effective isolation of anticoagulants, active ingredients and activators, preventing the solutions of each layer from mixing with each other and losing their effectiveness. Vitamin C provides antioxidant protection in PRP and prolongs the activity of growth factors. Calcium gluconate activates platelets to release growth factors and at the same time promotes blood coagulation as calcium ions. The three work together to improve the treatment effect. After centrifugation and shaking, a "PRP-vitamin C-calcium agent" composite solution can be obtained, which can be directly used for injection or external use. The operation is simple. The whole preparation process is completed in a closed PRP tube, reducing the chance of external contamination and ensuring the product quality and safety.

[0027] In this embodiment, the vacuum blood collection tube body 1 is inserted into the rubber stopper 2. The vacuum blood collection tube body 1 is a transparent vacuum blood collection tube one with a transparent structure. The transparent vacuum blood collection tube body 1 made of transparent material facilitates the observation and extraction of the positions of the separated layers.

[0028] In this embodiment, the rubber stopper 2 is made of rubber material.

[0029] In this embodiment, the calcium gluconate solution layer 3 is separated from the vitamin C solution layer 5 by the second separating gel layer 7, which can avoid the reaction and decomposition of the calcium gluconate solution layer 3 before centrifugal separation.

[0030] In this embodiment, the vitamin C solution layer 5 is separated from the ACDA solution layer 6 by the first separating gel layer 4, which can avoid the reaction and decomposition of the vitamin C solution layer 5 before centrifugal separation.

[0031] In this embodiment, the extraction method of the preparation tube for extracting platelet-rich plasma is as follows:

[0032] Step 1: Venous blood collection. Use a blood collection needle to puncture the vein of the patient, and then insert the other end of the needle tube of the blood collection needle through the rubber stopper 2 into the vacuum blood collection tube body 1. Under the action of the vacuum in the tube, automatically collect the required volume of blood;

[0033] Step 2: Blood shaking. Shake the vacuum blood collection tube body 1 after blood collection repeatedly to ensure that the ACDA solution layer 6 is evenly mixed with the collected blood;

[0034] Step 3: Centrifugal separation. Place the shaken vacuum blood collection tube body 1 in a centrifuge and perform centrifugation under set parameters to achieve centrifugal separation of various components in the blood;

[0035] Step 4: Shaking after centrifugation. The liquid in the vacuum blood collection tube body 1 after centrifugation is mainly divided into three layers. The upper layer is the plasma containing vitamin C and calcium gluconate, the middle layer is the separating gel, and the lower layer is the red blood cells. Since the platelets in the plasma are mainly located in the lower layer of the plasma after centrifugation, it is necessary to turn it over and shake it evenly to make the platelets evenly distributed in the plasma. At this time, platelet-rich plasma (PRP) containing vitamin C and calcium gluconate is also obtained;

[0036] Step 5: Extraction of platelet-rich plasma. After centrifugal separation, insert the needle of an external sterile syringe through the rubber stopper 2 into the vacuum blood collection tube, and extract the platelet-rich plasma containing vitamin C and calcium gluconate after centrifugation, then platelet-rich plasma containing vitamin C and calcium gluconate can be obtained.

[0037] In this embodiment, when shaking the vacuum blood collection tube body 1 after collecting blood in Step 2, it is shaken by the way of turning it up and down.

[0038] In this embodiment, when turning it over and shaking it evenly in Step 4, the vacuum blood collection tube body 1 is gently turned up and down more than twenty times, so that the platelets are evenly distributed in the plasma.

[0039] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A multi-layer structure platelet-rich plasma preparation tube, characterized in that: It includes a vacuum blood collection tube body (1), a rubber stopper (2) is installed at the top of the vacuum blood collection tube body (1), a calcium gluconate solution layer (3) is arranged at the inner bottom of the vacuum blood collection tube body (1), a second separating gel layer (7) is arranged above the calcium gluconate solution layer (3) in the vacuum blood collection tube body (1), a vitamin C solution layer (5) is arranged above the second separating gel layer (7) in the vacuum blood collection tube body (1), a first separating gel layer (4) is arranged above the vitamin C solution layer (5) in the vacuum blood collection tube body (1), and an ACDA solution layer (6) is arranged above the first separating gel layer (4).

2. The platelet-rich plasma preparation tube with a multi-layer structure according to claim 1, characterized in that: The vacuum blood collection tube body (1) is inserted into the rubber stopper (2), and the vacuum blood collection tube body (1) is of a transparent structure.

3. A multi-layer structure platelet-rich plasma preparation tube according to claim 1, characterized in that: The rubber stopper (2) is made of rubber material.

4. A multi-layer structure platelet-rich plasma preparation tube according to claim 1, characterized in that: The calcium gluconate solution layer (3) is separated from the vitamin C solution layer (5) by the second separating gel layer (7).

5. A multi-layer structure platelet-rich plasma preparation tube according to claim 1, characterized in that: The vitamin C solution layer (5) is separated from the ACDA solution layer (6) by the first separating gel layer (4).

6. A multi-layer structured platelet-rich plasma preparation tube according to any one of claims 1-5, characterized in that: The extraction method of this preparation tube for extracting platelet-rich plasma is as follows: Step 1: Venous blood collection. Use a blood collection needle to puncture the patient's vein, and then insert the other end of the needle tube of the blood collection needle through the rubber stopper (2) into the vacuum blood collection tube body (1). Under the action of the vacuum in the tube, automatically collect the required volume of blood. Step 2: Blood mixing. Shake the vacuum blood collection tube body (1) after blood collection repeatedly to ensure that the ACDA solution layer (6) is evenly mixed with the collected blood. Step 3: Centrifugal separation. Place the shaken vacuum blood collection tube body (1) in a centrifuge and perform centrifugation under set parameters to achieve centrifugal separation of various components in the blood. Step 4: Shake well after centrifugation. The liquid in the vacuum blood collection tube body (1) after centrifugation is mainly divided into three layers. The upper layer is plasma containing vitamin C and calcium gluconate, the middle layer is the separating gel, and the lower layer is red blood cells. Since the platelets in the plasma are mainly located in the lower layer of the plasma after centrifugation, it is necessary to turn it over and shake well to make the platelets evenly distributed in the plasma. At this time, platelet-rich plasma (PRP) containing vitamin C and calcium gluconate is also obtained. Step 5: Extraction of platelet-rich plasma. After centrifugal separation, insert the needle of an external sterile syringe through the rubber stopper (2) into the vacuum blood collection tube, and extract the platelet-rich plasma containing vitamin C and calcium gluconate after centrifugation, then platelet-rich plasma containing vitamin C and calcium gluconate can be obtained.

7. The extraction method of a multi-layer structured platelet-rich plasma preparation tube according to claim 6, characterized in that: In Step 2, when shaking the vacuum blood collection tube body (1) after collecting blood, it is shaken by turning it up and down.

8. A multi-layer structured platelet-rich plasma preparation tube according to claim 6, characterized in that: In Step 4, when turning it over and shaking well, the vacuum blood collection tube body (1) is gently turned up and down more than twenty times to make the platelets evenly distributed in the plasma.

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