Device and method for researching microplastid microbiome in soil based on micro-fluidic chip

Abstract

The invention provides a micro-fluidic chip-based device and a micro-fluidic chip-based method for researching micro-plastic intergranular microbiome in soil. The micro-fluidic chip-based device comprises a female die, and a plurality of culture dishes are mounted in the female die; a communicating mechanism is arranged in the female mold and comprises a plurality of first communicating grooves, a plurality of second communicating grooves and a plurality of third communicating grooves, the second communicating grooves are communicated with the plurality of third communicating grooves, and the third communicating grooves are communicated with the second communicating grooves. The side wall of the third communicating groove is obliquely communicated with the first communicating grooves, and the first communicating grooves are communicated with the bottom ends of the culture dishes; a partition plate is adhered to the interior of the female mold, and electromagnetic micro valves are mounted at the two ends of the female mold; the device and the method for researching the microplastid microbiome in the soil based on the micro-fluidic chip, provided by the invention, have the advantage that the microplastid microbiome is convenient to culture and observe.

Description

Technical Field

[0001] The present invention relates to the field of research on the microbiome in the plastisphere, and particularly to a device and method for researching the microbiome in soil microplastics based on a microfluidic chip. Background Art

[0002] Microplastics refer to plastic particles with a diameter less than 5 mm, whose particle size ranges from a few micrometers to a few millimeters. They are a heterogeneous mixture of plastic particles with diverse shapes and are often indistinguishable to the naked eye. As a relatively new pollutant, they serve as carriers for the colonization and transportation of harmful microorganisms and micro-animals. The hard surface of plastics provides an ideal environment for opportunistic microbial settlers to form biofilms and may provide protective niches that can support a variety of different microorganisms, known as "plastisphere". The plastisphere microbial community usually has a higher abundance of pathogenic bacteria and antibiotic resistance genes. The functional potential of microorganisms to metabolize organic compounds is enhanced in the plastisphere. The plastisphere microbial community in the aquatic environment often has a higher denitrification functional potential and may produce greenhouse gases such as nitrous oxide. The plastisphere microbial community poses a potential threat to ecosystem function and health.

[0003] To understand the harm of plastisphere microorganisms, it is necessary to conduct culture research on the plastisphere microbiome. However, the existing microbial culture dishes have a long culture time, and the plastisphere microbiome is buried in the soil and reproduces under pressure and hypoxia, making it difficult to form the growth environment required by plastisphere microorganisms in the culture dish, which is not conducive to the cultivation of microorganisms.

[0004] Therefore, it is necessary to provide a new device and method for researching the microbiome in soil microplastics based on a microfluidic chip to solve the above technical problems. Summary of the Invention

[0005] The technical problem solved by the present invention is to provide a device and method for researching the microbiome in soil microplastics based on a microfluidic chip, which is convenient for culturing and observing plastisphere microorganisms.

[0006] To solve the above technical problems, the device for studying the microbial community in the microplastic rhizosphere based on a microfluidic chip provided by the present invention includes: a master mold, inside which a plurality of culture dishes are installed, a soil layer is laid inside the master mold, and a cover glass is slidably connected to the surface of the master mold; a communication mechanism is provided inside the master mold, and the communication mechanism includes a first communication groove, a second communication groove, and a third communication groove. A plurality of the first communication grooves, the second communication groove, and the third communication groove are provided inside the master mold. The second communication groove communicates with a plurality of the third communication grooves. The side wall of the third communication groove is inclined to communicate with a plurality of the first communication grooves, and the first communication groove communicates with the bottom end of the culture dish; a partition is pasted inside the master mold, and the partition covers the surfaces of the first communication groove, the second communication groove, and the third communication groove; electromagnetic microvalves are installed at both ends of the master mold, one of the electromagnetic microvalves communicates with the inside of the partition, and the other electromagnetic microvalve is connected to a filtering mechanism, and the filtering mechanism is arranged on one side of the master mold.

[0007] Preferably, a plurality of through holes are provided on the surface of the partition, and the through holes are sleeved on the side walls of the culture dishes.

[0008] Preferably, a groove is provided at one end of the partition, and the groove communicates with the second communication groove.

[0009] Preferably, the depth of the second communication groove gradually increases from the middle of the master mold towards both ends of the master mold, and the second communication groove is perpendicular to the third communication groove.

[0010] Preferably, the width of the third communication groove gradually increases from the second communication groove towards the first communication groove, and the cross-section of the first communication groove is trapezoidal; on the surface of the same culture dish, the first communication grooves at the bottom end of the culture dish are arranged in a "V" shape.

[0011] Preferably, a rubber pad is installed at the bottom end of the cover glass, a layer of cover film is provided on the surface of the rubber pad, and the rubber pad and the cover film are slidably connected to the inner side wall of the master mold.

[0012] Preferably, the electromagnetic microvalve includes a valve body, the valve bodies are symmetrically installed at both ends of the master mold, a baffle is installed inside the top end of the valve body, and a magnetic rod is slidably connected inside the baffle; a spring is sleeved on the surface of the magnetic rod, and the top end of the spring abuts against the baffle; an electromagnet is installed inside the valve body, and the electromagnet adsorbs the side wall of the magnetic rod; a compression plug is installed at the bottom end of the magnetic rod, a valve film is installed inside the valve body, and the compression plug is slidably connected to the side wall of the valve film.

[0013] Preferably, the filtering mechanism includes connecting bars, both ends of the filter membrane are equipped with the connecting bars, the surface of the filter membrane is equipped with a plurality of elastic bars, one of the connecting bars abuts against the inside of the female mold, and the other connecting bar abuts against the cover film.

[0014] A method for studying the microplastic rhizosphere microbiome in soil based on a microfluidic chip specifically includes the following steps:

[0015] Step 1: Cover and paste the partition plate inside the female mold, the culture dish is sleeved on the surface of the through hole, and the groove on one side of the partition plate communicates with the second communication groove and the electromagnetic microvalve;

[0016] Step 2: Place the plastic layer inside the culture dish, inoculate microorganisms inside the culture dish, place the filtering mechanism on one side of the female mold, and then cover a layer of soil on the female mold; cover the cover glass on the surface of the female mold, and by applying pressure to the cover glass, simulate the reproduction environment of microorganisms in soil at different depths to improve the accuracy of the experiment;

[0017] Step 3: Connect the electromagnetic microvalve communicating with the groove to water, open the electromagnetic microvalve, and make the water carry salts into the inside of the groove and the second communication groove; then enter the inside of the culture dish upward through the third communication groove and the first communication groove to provide water and salts for the growth of microorganisms;

[0018] Step 4: The excess water inside the soil layer penetrates through the filter membrane and is discharged from the other electromagnetic microvalve. By controlling the water inflow and the salt water concentration, simulate the reproduction situation of microorganisms in different environments, which is convenient for people to observe and study.

[0019] Compared with the related technology, the device and method for studying the microplastic rhizosphere microbiome in soil based on a microfluidic chip provided by the present invention have the following beneficial effects:

[0020] The present invention provides a device and method for studying the microbiome of microplastics in soil based on a microfluidic chip. When culturing and observing the microbiome of microplastics, the plastic layer provides conditions for microorganisms, and the soil layer covers the plastic layer to simulate the environment in which microorganisms reproduce on the surface of the plastic layer, facilitating the observation of microorganisms. The culture dish is small in volume and large in quantity, capable of providing multiple microbial samples for observation. The microbial culture time is short and convenient, facilitating people's observation and detection. When culturing microorganisms, a cover glass is covered on the surface of the master mold, and by applying pressure to the cover glass, the reproduction environment of microorganisms at different depths in the soil is simulated, improving the accuracy of the experiment. When providing water and salts to microorganisms, since the depth of the second communication groove gradually increases from the center of the master mold towards both ends of the master mold, water flows towards both ends of the second communication groove, enabling the water to be evenly distributed inside the second communication groove, thereby facilitating the uniform entry of water into the third communication groove through the second communication groove. The width of the third communication groove gradually increases from the second communication groove towards the first communication groove. When water flows inside the third communication groove, the width of the third communication groove gradually increases, facilitating the entry of water into the end of the third communication groove, thereby enabling the water to be evenly distributed inside the third communication groove. When water enters the first communication groove through the third communication groove, the cross-section of the first communication groove is trapezoidal, facilitating the downward movement of water along the first communication groove into the culture dish. On the surface of the same culture dish, the first communication grooves at the bottom of the culture dish are arranged in a "V" shape, and water enters the same culture dish through two first communication grooves. The first communication grooves are inclined, facilitating the entry of water inside the third communication groove into the first communication groove. Moreover, the inclination angle of the first communication groove gradually increases from the second communication groove towards the first communication groove. As water flows forward inside the third communication groove, the first communication groove gradually tends to be parallel to the third communication groove, and the resistance of water entering the first communication groove gradually decreases, facilitating the entry of water into the first communication groove at the end, thereby enabling the water to enter each culture dish more evenly, making the internal environment of the culture dish consistent and conducive to the growth of microorganisms. Description of the Drawings

[0021] Figure 1 It is a schematic structural diagram of a preferred embodiment of the device and method for studying the microbiome of microplastics in soil based on a microfluidic chip provided by the present invention;

[0022] Figure 2 is Figure 1 an enlarged schematic diagram of the structure at location A shown;

[0023] Figure 3 is Figure 1Top view of the master mold structure shown

[0024] Figure 4 is Figure 1 Schematic diagram of the cover plate structure shown

[0025] Figure 5 is Figure 3 Schematic diagram of the internal structure of the first communication groove shown

[0026] Figure 6 is Figure 1 Schematic diagram of the internal structure of the electromagnetic microvalve shown

[0027] Reference numerals in the figure: 1, soil layer; 2, master mold; 21, culture dish; 22, plastic layer; 3, partition; 31, groove; 32, through hole; 4, electromagnetic microvalve; 41, valve body; 42, baffle; 43, spring; 44, magnetic rod; 45, compression plug; 46, valve film; 47, electromagnet; 5, cover glass; 51, rubber pad; 52, cover film; 6, filtering mechanism; 61, connecting strip; 62, filter membrane; 63, elastic strip; 7, communication mechanism; 71, first communication groove; 72, second communication groove; 73, third communication groove. Detailed implementation manners

[0028] The present invention will be further described below in conjunction with the accompanying drawings and implementation manners.

[0029] Please refer to Figures 1 to 6 , Figure 1 which is a schematic structural diagram of a preferred embodiment of the device and method for studying the microplastic-microbiome in soil based on a microfluidic chip provided by the present invention; Figure 2 is Figure 1 Enlarged schematic diagram of the structure at A shown Figure 3 is Figure 1 Top view of the master mold structure shown Figure 4 is Figure 1 Schematic diagram of the cover plate structure shown Figure 5 is Figure 3 Schematic diagram of the internal structure of the first communication groove shown Figure 6 is Figure 1Schematic diagram of the internal structure of the electromagnetic microvalve shown. The device for studying the microbial community in microplastics in soil based on a microfluidic chip includes: a master mold 2, inside which a plurality of culture dishes 21 are installed. A plastic layer 22 is laid inside the culture dish 21, and a soil layer 1 is laid inside the master mold 2. When culturing and observing the microbial community in microplastics, the plastic layer 22 provides conditions for the microorganisms, and the soil layer 1 covers the plastic layer 22, simulating the environment in which the microorganisms reproduce on the surface of the plastic layer 22, facilitating the observation of the microorganisms. The culture dishes 21 are small in volume and large in number, can provide multiple microbial samples for observation, and the microbial culture time is short and convenient, facilitating people's observation and detection.

[0030] A cover glass 5 is slidably connected to the surface of the master mold 2; a rubber pad 51 is installed at the bottom end of the cover glass 5, and a cover film 52 is provided on the surface of the rubber pad 51. The rubber pad 51 and the cover film 52 are slidably connected to the inner side wall of the master mold 2, covering the cover glass 5 on the surface of the master mold 2. The rubber pad 51 and the cover film 52 enter the inside of the master mold 2. As the cover glass 5 descends, the cover film 52 contacts the soil layer 1 and presses the soil layer 1 downward, thereby applying pressure to the plastic layer 22 and the microorganisms on its surface, thus simulating the pressure borne by the microorganisms when reproducing at different depths in the soil. People simulate the reproduction environment of the microorganisms in the soil by applying pressure to the cover glass 5, improving the accuracy of the experiment; and the rubber pad 51 abuts against the inner side wall of the master mold 2, closing the inside of the master mold 2. When the cover film 52 is disengaged from the soil layer 1, pressure is evenly applied to the soil layer 1, making the pressure evenly distributed inside the soil layer 1.

[0031] A communication mechanism 7 is provided inside the master mold 2. The communication mechanism 7 includes a first communication groove 71, a second communication groove 72, and a third communication groove 73. A plurality of the first communication grooves 71, the second communication groove 72, and the third communication groove 73 are provided inside the master mold 2. The second communication groove 72 communicates with a plurality of the third communication grooves 73. The side wall of the third communication groove 73 is inclined to communicate with a plurality of the first communication grooves 71, and the first communication groove 71 communicates with the bottom end of the culture dish 21; when culturing microorganisms, water and salts enter the inside of the second communication groove 72, and then enter the inside of the culture dish 21 through the third communication groove 73 and the first communication groove 71, providing water and salts for the growth of the microorganisms.

[0032] A partition plate 3 is pasted inside the master mold 2, and the partition plate 3 covers the surfaces of the first communication groove 71, the second communication groove 72, and the third communication groove 73; a plurality of through holes 32 are provided on the surface of the partition plate 3, and the through holes 32 are sleeved on the side wall of the culture dish 21. In order to facilitate the partition plate 3 to pass through the side wall of the culture dish 21 and contact the inner side wall of the master mold 2, and at the same time cover the surfaces of the first communication groove 71, the second communication groove 72, and the third communication groove 73, it is convenient for water to flow inside the first communication groove 71, the second communication groove 72, and the third communication groove 73.

[0033] One end of the partition plate 3 is provided with a groove 31, and the groove 31 communicates with the second communication groove 72. In order to facilitate water to enter the inside of the groove 31 and then enter the inside of the second communication groove 72 through the groove 31.

[0034] Moreover, the second communication groove 72 and the third communication groove 73 are perpendicular to each other. When water flows inside the second communication groove 72, the depth of the second communication groove 72 gradually increases from the middle of the master mold 2 towards both ends of the master mold 2, so that water flows towards both ends of the second communication groove 72, and the water is evenly distributed inside the second communication groove 72, thereby facilitating the water to evenly enter the inside of the third communication groove 73 through the second communication groove 72.

[0035] The inclination angle of the first communication groove 71 and the width of the third communication groove 73 gradually increase along the direction of the second communication groove 72 towards the first communication groove 71. When water flows inside the third communication groove 73, the width of the third communication groove 73 gradually increases, facilitating the entry of water into the end of the third communication groove 73, thereby enabling the water to be evenly distributed inside the third communication groove 73; when water enters the inside of the first communication groove 71 through the third communication groove 73, the cross-section of the first communication groove 71 is trapezoidal, facilitating the downward movement of water along the first communication groove 71 into the inside of the culture dish 21. And on the surface of the same culture dish 21, the first communication groove 71 at the bottom end of the culture dish 21 is arranged in a "V" shape, and water enters the inside of the same culture dish 21 through two first communication grooves 71. The first communication groove 71 is inclined, facilitating the entry of water inside the third communication groove 73 into the inside of the first communication groove 71; and the inclination angle of the first communication groove 71 gradually increases along the direction of the second communication groove 72 towards the first communication groove 71. As water flows forward inside the third communication groove 73, the first communication groove 71 gradually tends to be parallel to the third communication groove 73, and the resistance of water entering the first communication groove 71 gradually decreases, facilitating the entry of water into the first communication groove 71 at the end, thereby enabling the water to enter the inside of each culture dish 21 more evenly, making the internal environment of the culture dish 21 consistent and being conducive to the growth of microorganisms.

[0036] Electromagnetic micro-valves 4 are installed at both ends of the master mold 2. One of the electromagnetic micro-valves 4 communicates with the inside of the partition plate 3, and the other electromagnetic micro-valve 4 is connected to a filtering mechanism 6, and the filtering mechanism 6 is arranged on one side of the master mold 2. The electromagnetic micro-valve 4 includes a valve body 41, the valve bodies 41 are symmetrically installed at both ends of the master mold 2, a baffle 42 is installed inside the top end of the valve body 41, and a magnetic rod 44 is slidably connected inside the baffle 42; a spring 43 is sleeved on the surface of the magnetic rod 44, and the top end of the spring 43 abuts against the baffle 42; an electromagnet 47 is installed inside the valve body 41, and the electromagnet 47 adsorbs the side wall of the magnetic rod 44; a compression plug 45 is installed at the bottom end of the magnetic rod 44, a valve film 46 is installed inside the valve body 41, and the compression plug 45 is slidably connected to the side wall of the valve film 46; in order to close the electromagnetic micro-valve 4 when needed, the electromagnet 47 is turned on, the electromagnet 47 adsorbs the magnetic rod 44 downward, the magnetic rod 44 drives the compression plug 45 to move downward to stretch the spring 43, and the compression plug 45 squeezes the valve film 46 downward, causing the valve film 46 to move to close the inside of the valve body 41.

[0037] The filtering mechanism 6 includes a connecting strip 61. Both ends of the filter membrane 62 are equipped with the connecting strip 61. A plurality of elastic strips 63 are installed on the surface of the filter membrane 62. One connecting strip 61 abuts against the inside of the female mold 2, and the other connecting strip 61 abuts against the covering film 52. To facilitate the connecting strip 61 to fix the filter membrane 62 on one side of the culture dish 21 and prevent the soil inside the culture dish 21 from flowing out; and when the rubber pad 31 moves downward, the rubber pad 31 squeezes the connecting strip 61 downward, the two connecting strips 61 approach each other, the connecting strip 61 squeezes the elastic strip 61 and the filter membrane 62 outward, the elastic strip 61 deforms outward, so that the filter membrane 62 expands outward to squeeze the soil layer 1 and push the soil layer 1 away to prevent soil loss; at the same time, the contact area between the filter membrane 62 and the soil layer 1 is increased, facilitating the water inside the soil layer 1 to penetrate through the filter membrane 62 and flow out from inside the valve body 41.

[0038] A method for studying the microplastic rhizosphere microbiome in soil based on a microfluidic chip specifically includes the following steps.

[0039] Step 1: Cover the partition plate 3 inside the female mold 2. The culture dish 21 is sleeved on the surface of the through hole 32. The groove 31 on one side of the partition plate 3 communicates with the second communication groove 72 and the electromagnetic microvalve 4.

[0040] Step 2: Place the plastic layer 22 inside the culture dish 21, inoculate microorganisms inside the culture dish 21, place the filtering mechanism 6 on one side of the female mold 2, and then cover a layer of soil on the female mold 2; cover the cover glass 5 on the surface of the female mold 2. The rubber pad 51 and the covering film 52 enter the inside of the female mold 2. As the cover glass 5 descends, the covering film 52 contacts the soil layer 1 and squeezes the soil layer 1 downward, thereby applying pressure to the plastic layer 22 and the microorganisms on its surface, thus simulating the pressure borne by microorganisms when reproducing at different depths in the soil. People simulate the reproduction environment of microorganisms in the soil by the pressure applied to the cover glass 5 to improve the accuracy of the experiment; and the rubber pad 51 abuts against the inner side wall of the female mold 2 to seal the inside of the female mold 2. The covering film 52 is separated from the soil layer 1, thereby applying uniform pressure to the soil layer 1 and making the pressure evenly distributed inside the soil layer 1.

[0041] Step 3: Connect the electromagnetic microvalve 4 communicating with the groove 31 to water, open the electromagnetic microvalve 4, so that water carries salts into the inside of the groove 31 and the second communication groove 72; then enter the inside of the culture dish 21 upward through the third communication groove 73 and the first communication groove 71 to provide water and salts for the growth of microorganisms.

[0042] Step Four: Excess water inside the soil layer 1 penetrates through the filter membrane 62 and is discharged from the other electromagnetic micro-valve 4; when the rubber pad 31 moves downward to squeeze the soil layer 1, the rubber pad 31 squeezes the connecting bar 61 downward, the two connecting bars 61 approach each other, the connecting bar 61 squeezes the elastic strip 61 and the filter membrane 62 outward, the elastic strip 61 deforms outward, causing the filter membrane 62 to expand outward and squeeze the soil layer 1, pushing the soil layer 1 away to prevent soil loss; at the same time, the contact area between the filter membrane 62 and the soil layer 1 is increased, facilitating the water inside the soil layer 1 to penetrate through the filter membrane 62 and flow out from inside the valve body 41.

[0043] The above are only the embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of the present invention.

Claims

1. An apparatus for studying the microbial community in the rhizosphere of microplastics in soil based on a microfluidic chip, characterized in that, Comprising: A master mold (2), multiple culture dishes (21) are installed inside the master mold (2), a soil layer (1) is laid inside the master mold (2), and a cover glass (5) is slidably connected to the surface of the master mold (2); A communication mechanism (7) is provided inside the master mold (2). The communication mechanism (7) includes a first communication groove (71), a second communication groove (72), and a third communication groove (73). Multiple of the first communication grooves (71), the second communication grooves (72), and the third communication grooves (73) are provided inside the master mold (2). The second communication groove (72) communicates with multiple of the third communication grooves (73). The side wall of the third communication groove (73) is inclined to communicate with multiple of the first communication grooves (71), and the first communication groove (71) communicates with the bottom end of the culture dish (21); A partition (3) is pasted inside the master mold (2), and the partition (3) covers the surfaces of the first communication groove (71), the second communication groove (72), and the third communication groove (73); Electromagnetic micro-valves (4) are installed at both ends of the master mold (2). One of the electromagnetic micro-valves (4) communicates with the inside of the partition (3), and the other electromagnetic micro-valve (4) is connected to a filtering mechanism (6), and the filtering mechanism (6) is arranged on one side of the master mold (2).

2. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 1, wherein, Multiple through holes (32) are provided on the surface of the partition (3), and the through holes (32) are sleeved on the side wall of the culture dish (21).

3. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 2, wherein, A groove (31) is provided at one end of the partition (3), and the groove (31) communicates with the second communication groove (72).

4. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 3, wherein, The depth of the second communication groove (72) gradually increases from the middle of the master mold (2) towards both ends of the master mold (2), and the second communication groove (72) is perpendicular to the third communication groove (73).

5. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 4, wherein, The width of the third communication groove (73) gradually increases from the second communication groove (72) towards the first communication groove (71). The cross-section of the first communication groove (71) is trapezoidal; On the surface of the same culture dish (21), the first communication groove (71) at the bottom end of the culture dish (21) is arranged in a "V" shape.

6. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 5, characterized in that, A rubber pad (51) is installed at the bottom end of the cover glass (5). A layer of cover film (52) is provided on the surface of the rubber pad (51), and the rubber pad (51) and the cover film (52) are slidably connected to the inner side wall of the master mold (2).

7. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 6, characterized in that, The electromagnetic micro-valve (4) includes a valve body (41). The valve bodies (41) are symmetrically installed at both ends of the female mold (2). A baffle (42) is installed inside the top end of the valve body (41). A magnetic rod (44) is slidably connected inside the baffle (42); a spring (43) is sleeved on the surface of the magnetic rod (44), and the top end of the spring (43) abuts against the baffle (42); an electromagnet (47) is installed inside the valve body (41), and the electromagnet (47) adsorbs the side wall of the magnetic rod (44); a compression plug (45) is installed at the bottom end of the magnetic rod (44), a valve film (46) is installed inside the valve body (41), and the compression plug (45) is slidably connected to the side wall of the valve film (46).

8. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 7, wherein The filtering mechanism (6) includes a connecting strip (61). The connecting strips (61) are installed at both ends of the filter membrane (62). A plurality of elastic strips (63) are installed on the surface of the filter membrane (62). One of the connecting strips (61) abuts against the inside of the female mold (2), and the other connecting strip (61) abuts against the cover film (52).

9. The device for studying the microbial community in the microplastic rhizosphere of soil based on a microfluidic chip according to claim 8, wherein It includes a method for studying the microplastic rhizosphere microbiome in soil based on a microfluidic chip, specifically including the following steps: Step 1: Cover and paste the partition plate (3) inside the female mold (2). The culture dish (21) is sleeved on the surface of the through hole (32). The groove (31) on one side of the partition plate (3) communicates with the second communication groove (72) and the electromagnetic micro-valve (4); Step 2: Place the plastic layer (22) inside the culture dish (21), inoculate microorganisms inside the culture dish (21), place the filtering mechanism (6) on one side of the female mold (2), and then cover a layer of soil on the female mold (2); Cover the cover glass (5) on the surface of the female mold (2). By applying pressure to the cover glass (5), simulate the reproduction environment of microorganisms in soil at different depths and improve the accuracy of the experiment; Step 3: Connect water to the electromagnetic micro-valve (4) communicating with the groove (31), open the electromagnetic micro-valve (4), and make the water carry salts into the groove (31) and the second communication groove (72); Then enter the culture dish (21) upward through the third communication groove (73) and the first communication groove (71) to provide water and salts for the growth of microorganisms; Step 4: The excess water inside the soil layer (1) penetrates through the filter membrane (62) and is discharged from the other electromagnetic micro-valve (4). By controlling the water inflow and brine concentration, simulate the reproduction situation of microorganisms in different environments for people to observe and study.

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