Photobioreactor device for microalgae culture

Abstract

The invention discloses a photobioreactor device for microalgae culture, and relates to the technical field of biological growth containers, the photobioreactor device comprises a frame, a mounting rack I, a controller and an incubator shell, a reaction barrel is rotatably arranged in the incubator shell, and a plurality of lantern boxes are sequentially arranged in the reaction barrel from top to bottom; the top of the outer side of each lantern box is provided with a drainage ring, the bottom end of the bottommost lantern box is fixedly provided with a fourth flow guide pipeline, and a plurality of first exhaust pipes used for releasing pressure in the reaction barrel are internally provided with sampling assemblies. Under the conditions that algae cultivation is not affected and waste gas leakage is little, algae samples are obtained from different positions in the photobioreactor device for multiple times, and the culture effect of the photobioreactor device can be conveniently detected.

Description

Technical Field

[0001] The present invention relates to the technical field of biological growth containers, and specifically to a photobioreactor device for microalgae cultivation. Background Art

[0002] Microalgae are generally photosynthetic autotrophs with great growth potential. They are producers in the ecosystem and an important part of the primary productivity in the biosphere. Some microalgae grow rapidly and can absorb a large amount of nutrients such as nitrogen and phosphorus in water bodies, becoming a new type of organism for treating water pollution.

[0003] Microalgae are a type of photosynthetic microorganism that can grow rapidly. They belong to prokaryotic or eukaryotic photosynthetic microorganisms and usually have a single-celled or simple multi-celled structure. Microalgae carry out autotrophic growth and photosynthetic carbon fixation through photosynthesis, absorbing and converting water and CO2 into carbohydrates such as oils and starches, while releasing oxygen.

[0004] Microalgae photosynthesis is a complex biochemical conversion process involving the transport and conversion of substances. Its raw materials are H2O and CO2, which are converted into products such as O2, starch, and oils through photosynthesis. While absorbing greenhouse gases, microalgae can produce organic substances with economic value, thus providing potential solutions to environmental and energy problems.

[0005] The microalgae bioreactor is used for the industrial cultivation of the marine product "microalgae". For the convenience of light energy supply, the reactor uses a heat-resistant tempered glass cylinder for lighting and an LED light source, which has the effects of low energy consumption and low radiant heat of the light source.

[0006] However, in the application process of existing photobioreactors, although a special sampling channel is set for sampling, during the sampling process, it is easy to cause the leakage of waste gas in the photobioreactor, resulting in the escape of waste gas and environmental pollution. Moreover, the position of the sampling channel is fixed, resulting in sampling only from one position. After multiple samplings, the growth situation of algae at the sample location is different from that at other positions, and the obtained samples cannot accurately reflect the growth situation of algae at different time periods. Summary of the Invention

[0007] The purpose of the present invention is to provide a photobioreactor device for microalgae cultivation to solve the problems raised in the prior art.

[0008] To achieve the above object, the present invention provides the following technical solution: A photobioreactor device for microalgae cultivation, comprising a vehicle frame, a first mounting frame, a controller, and a cultivator housing. A reaction barrel is rotatably arranged inside the cultivator housing. A plurality of lantern boxes are sequentially arranged inside the reaction barrel from top to bottom. Drainage rings are arranged at the tops of the outer sides of the plurality of lantern boxes. A fourth diversion pipe is fixedly installed at the bottom end of the lowermost lantern box. A plurality of round holes are formed in the outer side of the fourth diversion pipe. A first sealing plate is rotatably connected to the top of the inner cavity of the reaction barrel. The bottom of the fourth diversion pipe is connected to a water and gas supply assembly. A stirring frame is arranged at the top of the uppermost lantern box. A transmission assembly is arranged at the top of the stirring frame. A second sealing plate is fixedly installed at the top of the inner cavity of the cultivator housing. A confluence box is fixedly installed on the second sealing plate. A plurality of first exhaust pipes penetrate through the confluence box. The first exhaust pipes extend to the top of the inner cavity of the reaction barrel. Sampling assemblies are inserted inside the plurality of first exhaust pipes. The sampling assembly includes an external thread member threadedly connected to the top of the inner cavity of the first exhaust pipe, a telescopic rod fixedly installed inside the external thread member, an operating rod fixedly installed at the top of the telescopic rod, and two spring-type telescopic rods fixedly installed at the bottom end of the operating rod. A third sealing plate is fixedly installed at the bottom end of one of the spring-type telescopic rods. A fourth sealing plate is fixedly installed at the bottom end of the other spring-type telescopic rod.

[0009] Preferably, two adjacent lantern boxes are fixedly installed together. The drainage ring is fixedly installed inside the reaction barrel. A third diversion pipe is fixedly installed at the top of the uppermost lantern box. The top end of the third diversion pipe extends to the top of the uppermost drainage ring.

[0010] Preferably, the water and gas supply assembly includes a fifth diversion pipe fixedly installed at the bottom end of the fourth diversion pipe and a second three-way valve fixedly installed at the bottom end of the fifth diversion pipe. The fourth diversion pipe rotatably penetrates through the bottom of the inner cavity of the reaction barrel. The normally closed end of the second three-way valve is fixedly installed with a sixth diversion pipe. One end of the sixth diversion pipe extends to the bottom of the vehicle frame. The sixth diversion pipe and the second three-way valve are both fixedly connected to the vehicle frame. One end of the sixth diversion pipe is fixedly connected to a first three-way valve. A plurality of short rods are fixedly installed between the first three-way valve and the vehicle frame. The normally open end of the first three-way valve is fixedly installed with a second diversion pipe. The normally closed end of the first three-way valve is fixedly installed with a first diversion pipe.

[0011] Preferably, a check valve is fixedly installed at the normally open end of the second three-way valve. A seventh diversion pipe is fixedly installed on one side of the check valve. The top end of the seventh diversion pipe penetrates through the second sealing plate and is fixedly installed with a flow detector. A flow guide pipe is fixedly installed at the air inlet end of the flow detector.

[0012] Preferably, the first mounting bracket is fixedly installed on one side of the top of the vehicle frame, the controller is fixedly installed on one side of the first mounting bracket, the culture vessel housing is fixedly installed on the top of the vehicle frame, a plurality of fifth mounting brackets are rotatably installed on the outer side of the reaction barrel, and the fifth mounting brackets are fixedly installed inside the culture vessel housing.

[0013] Preferably, the transmission assembly includes a second mounting bracket arranged on the top of the stirring frame, an electric telescopic rod fixedly installed inside the second mounting bracket, and a gearbox arranged on the outer side of the second mounting bracket. The bottom end of the second mounting bracket penetrates through the first sealing plate and the second sealing plate and is rotatably connected to the first sealing plate and the second sealing plate. The piston end of the electric telescopic rod is fixedly installed on the top of the stirring frame.

[0014] Preferably, two third mounting brackets are fixedly installed on one side of the first mounting bracket, a servo motor is fixedly installed between the two third mounting brackets, the output end of the servo motor is fixedly connected to the input end of the gearbox, the gearbox is fixedly installed between the two third mounting brackets, and the second mounting bracket penetrates through the gearbox and is fixedly connected to the gear at the transmission end inside the gearbox.

[0015] Preferably, a plurality of metal plates are fixedly installed on the outer side of the second mounting bracket, an electromagnet is rotatably connected to the bottom of the first sealing plate, the metal plates are arranged inside the electromagnet, a plurality of connecting side plates are fixedly installed between the outer side of the electromagnet and the reaction barrel, and a plurality of fixed round rods are fixedly installed between the first sealing plate and the second sealing plate.

[0016] Preferably, the operating rod is slidably installed inside the external thread member and is in interference fit with the external thread member. A rubber tube is fixedly installed on the outer side of the external thread member. The housings of the two spring-type telescopic rods penetrate through the external thread member. Slots are formed at the bottoms of the third sealing plate and the fourth sealing plate. A limiting ring is fixedly installed at the bottom of the inner cavity of the first exhaust pipe.

[0017] Preferably, a fourth mounting bracket is fixedly installed on the top of the confluence box. A plurality of nuts are fixedly installed inside the fourth mounting bracket. The plurality of nuts are respectively sleeved on the outer sides of the plurality of first exhaust pipes in a threaded manner. Mounting grooves three are arranged at the bottoms of the plurality of nuts. The plurality of mounting grooves three are all formed on the top of the confluence box. Mounting grooves one are arranged at the bottoms of the plurality of mounting grooves three. The plurality of mounting grooves one are all formed on the top of the second sealing plate. Mounting grooves two are arranged at the bottoms of the plurality of mounting grooves one. The plurality of mounting grooves two are all formed on the top of the first sealing plate. A gas guiding groove is formed on the outer side of the first exhaust pipe. The gas guiding groove is arranged inside the confluence box. A second exhaust pipe is fixedly installed on the top of the confluence box. The second exhaust pipe penetrates through the fourth mounting bracket.

[0018] Compared with the prior art, the beneficial effects of the present invention are: 1. In this application, a sampling assembly is provided inside each of multiple exhaust pipes for relieving pressure inside the reaction barrel. During the process of cultivating algae using wastewater and waste gas in a photobioreactor device, it is possible to obtain algae samples from different positions inside the photobioreactor device multiple times with little impact on algae cultivation and minimal waste gas leakage, facilitating the detection of the cultivation effect of the photobioreactor device.

[0019] 2. In this application, after the solution in the drainage pipe moves upward and enters the reaction barrel during the aeration process, the solution in the reaction barrel moves downward and enters the drainage pipe through the round holes. Due to the long-term aeration, the solution that enters the drainage pipe will then enter the bottom cavity of the reaction barrel again due to aeration, causing the water bodies inside and outside the drainage pipe to circulate. The circulating solution has its flow trajectory changed by multiple drainage rings. By changing the flow trajectory of the solution inside the reaction barrel, the flashing effect of the algae in the reaction barrel is enhanced, improving the photosynthesis and biomass production of the algae, and effectively increasing the carbon fixation rate and mass transfer coefficient of the algae.

[0020] 3. In this application, a photobioreactor device composed of a culture vessel housing, a reaction barrel, multiple lantern boxes, multiple drainage rings, a transmission assembly, etc. enables algae to efficiently absorb and utilize CO2, which is beneficial for reducing CO2 emissions, helping to slow down greenhouse gas emissions, and thus having a positive impact on environmental protection and climate change response. Combining wastewater treatment, CO2 absorption, and algae cultivation can reduce operating costs and improve resource utilization efficiency, thereby achieving a return on technology investment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic structural diagram of the present invention; Figure 2 is a partial structural diagram of the culture vessel housing of the present invention; Figure 3 is Figure 2 an enlarged view of the structure at B of Figure 4 is a partial structural diagram of the reaction barrel of the present invention; Figure 5 is Figure 4 an enlarged view of the structure at A of Figure 6 is Figure 4 an enlarged view of the structure at C of Figure 7 is a schematic structural diagram of the seventh diversion pipeline of the present invention; Figure 8 is a schematic structural diagram of the second sealing plate of the present invention; Figure 9 is a schematic structural diagram of the fourth mounting bracket of the present invention; Figure 10 is a schematic structural diagram of the third mounting bracket of the present invention; Figure 11 It is a partial structural schematic diagram of the second mounting bracket of the present invention; Figure 12 It is a partial structural schematic diagram of the busbar box of the present invention; Figure 13 It is a partial structural schematic diagram of the first exhaust pipe of the present invention; Figure 14 It is a partial structural schematic diagram of the third sealing plate of the present invention.

[0022] Wherein: 1. Frame; 2. First mounting bracket; 3. Controller; 4. First three-way valve; 5. First diversion pipeline; 6. Second diversion pipeline; 7. Incubator housing; 8. Reaction barrel; 9. Lantern box; 10. Third diversion pipeline; 11. Fourth diversion pipeline; 12. Fifth diversion pipeline; 13. Second three-way valve; 14. Sixth diversion pipeline; 15. Check valve; 16. Seventh diversion pipeline; 17. Flow detector; 18. Eighth diversion pipeline; 19. First sealing plate; 20. Second sealing plate; 21. Stirring frame; 22. Second mounting bracket; 23. Electric telescopic rod; 24. Gear box; 25. Servo motor; 26. Third mounting bracket; 27. Busbar box; 28. Fourth mounting bracket; 29. First exhaust pipe; 30. Air guide groove; 31. First mounting groove; 32. Second mounting groove; 33. Third mounting groove; 34. External thread part; 35. Telescopic rod; 36. Operating rod; 37. Rubber tube; 38. Spring-type telescopic rod; 39. Third sealing plate; 40. Fourth sealing plate; 41. Limit ring; 42. Card slot; 43. Metal plate; 44. Electromagnet; 45. Connecting side plate; 46. Fixed round rod; 47. Fifth mounting bracket; 48. Drainage ring; 49. Round hole; 50. Second exhaust pipe. Specific embodiments

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Embodiment: As Figures 1 - 14 shown, the present invention provides a technical solution for a photobioreactor device for microalgae cultivation: Specifically, in the present application, structures such as the incubator housing 7, the reaction barrel 8, the lantern box 9, the seventh diversion pipeline 16, the fifth mounting bracket 47, and the drainage ring 48 are all made of plexiglass with a light transmittance of more than 80%, having good light transmittance; The first mounting bracket 2 is fixedly installed on one side of the top of the frame 1, and the controller 3 is fixedly installed on one side of the first mounting bracket 2. The controller 3 is used to control the water and gas supply component and the transmission component to work.

[0025] Specifically, the outer shell 7 of the incubator is fixedly installed on the top of the vehicle frame 1. A plurality of fifth mounting brackets 47 are rotatably installed on the outer side of the reaction barrel 8 arranged inside the outer shell 7 of the incubator, and the fifth mounting brackets 47 are fixedly installed inside the outer shell 7 of the incubator. Therefore, the vertical position of the reaction barrel 8 inside the outer shell 7 of the incubator remains unchanged. A plurality of lantern boxes 9 are sequentially arranged from top to bottom inside the reaction barrel 8. Two adjacent lantern boxes 9 are fixedly installed together. The plurality of lantern boxes 9 form a drainage pipe. Drainage rings 48 are arranged on the tops of the outer sides of the plurality of lantern boxes 9. The drainage rings 48 are fixedly installed inside the reaction barrel 8. A third diversion pipe 10 is fixedly installed on the top of the uppermost lantern box 9. The top end of the third diversion pipe 10 extends to the top of the uppermost drainage ring 48. Therefore, the water flow inside the drainage pipe can move to the top of the uppermost drainage ring 48. A fourth diversion pipe 11 is fixedly installed at the bottom end of the lowermost lantern box 9. A plurality of round holes 49 opened on the outer side of the fourth diversion pipe 11 are all arranged at the bottom of the inner cavity of the reaction barrel 8. The drainage pipe is communicated with the bottom of the inner cavity of the reaction barrel 8 through the plurality of round holes 49.

[0026] In the water and gas supply assembly, a fifth diversion pipe 12 is fixedly installed at the bottom end of the fourth diversion pipe 11. A normally closed end of a two-way valve 13 fixedly installed at the bottom end of the fifth diversion pipe 12 is fixedly installed with a sixth diversion pipe 14. One end of the sixth diversion pipe 14 extends to the bottom of the vehicle frame 1 and is fixedly connected to a first three-way valve 4. A second diversion pipe 6 fixedly installed at the normally open end of the first three-way valve 4 is connected to a water supply system. The water supply system supplies waste water. A first diversion pipe 5 fixedly installed at the normally closed end of the first three-way valve 4 is connected to a microalgae recovery system. A check valve 15 is fixedly installed at the normally open end of the two-way valve 13. The check valve 15 is provided to prevent the liquid inside the reaction barrel 8 from flowing out through the normally open end of the two-way valve 13. A seventh diversion pipe 16 is fixedly installed on one side of the check valve 15. The top end of the seventh diversion pipe 16 penetrates through a second sealing plate 20 and is fixedly installed with a flow detector 17. An eighth diversion pipe 18 fixedly installed at the air inlet end of the flow detector 17 is used to connect to an exhaust gas supply system. The exhaust gas is exhaust gas containing a high concentration of CO2.

[0027] When the water supply system works for the first time, algae are put into the waste water. The water supply system conveys a solution containing a certain amount of microalgae to the inside of the drainage pipe and the reaction barrel 8 through the second diversion pipe 6, the first three-way valve 4, the sixth diversion pipe 14, the fifth diversion pipe 12, the fourth diversion pipe 11 and the round holes 49. When the height of the solution inside the reaction barrel 8 reaches the top of the uppermost drainage ring 48 but does not submerge the top end of the third diversion pipe 10, the water supply is stopped. The normally closed end of the two-way valve 13 is controlled to close, and the normally open end of the two-way valve 13 is controlled to open. The sixth diversion pipe 14 is no longer communicated with the inside of the reaction barrel 8. The normally closed end of the first three-way valve 4 is controlled to open, and the inside of the first three-way valve 4 is emptied. Subsequently, the exhaust gas supply system is controlled to operate, and exhaust gas with a certain air pressure is supplied into the interior of the diversion pipe four 11 through the diversion pipe eight 18, the flow detector 17, the diversion pipe seven 16, the one-way valve 15, the normally open end of the three-way valve two 13, and the diversion pipe five 12. The exhaust gas rushes into the interior of the drainage pipe formed by multiple lantern boxes 9 through the diversion pipe four 11, and an aeration phenomenon occurs from bottom to top in the drainage pipe. During the aeration process, part of the solution inside the drainage pipe moves upward and leaves from the top end of the diversion pipe three 10, and the solution falls onto the top of the bottommost drainage ring 48; Except for the topmost drainage ring 48, the rest of the drainage rings 48 are arranged in the depressions of the drainage pipe. There is a certain space between the drainage ring 48 and the drainage pipe to meet the need for solution flow. The round hole 49 opened on the outer side of the diversion pipe four 11 is arranged at the bottom of the inner cavity of the reaction barrel 8. After the solution moving upward inside the drainage pipe enters the interior of the reaction barrel 8 during the aeration process, the solution inside the reaction barrel 8 moves downward and enters the interior of the drainage pipe through the round hole 49. Due to the long-term aeration, the solution will enter the interior of the drainage pipe and then enter the bottom of the inner cavity of the reaction barrel 8 again due to aeration, causing the water body inside and outside the drainage pipe to circulate; The circulating solution is changed in the water body flow trajectory by multiple drainage rings 48. By changing the flowing trajectory of the solution inside the reaction barrel 8, the flashing effect of algae in the reaction barrel 8 is improved, the photosynthesis and biomass yield of algae are increased, and the carbon fixation rate and mass transfer coefficient of algae are effectively improved.

[0028] In summary, when the exhaust gas moves upward inside the drainage pipe, it drives the solution at the bottom of the inner cavity of the reaction barrel 8 and the bottom of the inner cavity of the drainage pipe to rise inside the drainage pipe, and the solution outside the drainage pipe descends, forming a circulating water body. Due to the structural design of the multiple lantern boxes 9 that make up the drainage pipe and the existence of multiple drainage rings 48 outside the drainage pipe, the solution bends upward and downward in the longitudinal section. At the same time, light shines on the outer shell 7 of the incubator, and the closer to the central axis of the reaction barrel 8, the smaller the light intensity, which improves the flashing effect of algae and ensures the quality of algae reproduction.

[0029] Specifically, during the process of delivering waste gas into the drainage pipe, the servo motor 25 in the drive assembly is controlled to operate. Two mounting brackets three 26 are fixedly installed on one side of the mounting bracket one 2. Both the gearbox 24 and the servo motor 25 are fixedly installed between the two mounting brackets three 26, and the output end of the servo motor 25 is fixedly connected to the input end of the gearbox 24. The mounting bracket two 22 penetrates through the sealing plate one 19 and the sealing plate two 20 and is rotatably connected to the sealing plate one 19 and the sealing plate two 20. The top end of the mounting bracket two 22 penetrates through the gearbox 24 and is fixedly connected to the gear at the transmission end inside the gearbox 24. Therefore, after controlling the servo motor 25 to operate at a low power, the servo motor 25 outputs a rotational force to the gearbox 24. After the rotational force is redirected and speed-changed by the gearbox 24, it acts on the mounting bracket two 22. The mounting bracket two 22 rotates to drive the fixedly installed electric telescopic rod 23 inside to rotate. The piston end of the electric telescopic rod 23 is slidably connected to the outer shell of the electric telescopic rod 23, and the piston end of the electric telescopic rod 23 cannot rotate relative to the inside of the outer shell of the electric telescopic rod 23. The stirring frame 21 fixedly installed at the piston end of the electric telescopic rod 23 rotates synchronously with the mounting bracket two 22. Since the stirring frame 21 is arranged at the top of the diversion pipe three 10, the rotating stirring frame 21 disperses and mixes the waste gas and the solution that move upward inside the diversion pipe three 10 due to aeration, increasing the contact opportunity between the algae and CO2 and helping to improve the growth rate of the algae; Through the lantern-like structure design of the lantern box 9, small vortices can be formed when the solution flows, increasing the contact area between the CO2 gas in the waste gas and the solution, thereby improving the absorption efficiency of CO2 by the algae. The multiple drainage rings 48 arranged on the outer side of the drainage pipe guide the movement path of the solution, enabling the solution to form a complex flow structure inside the reaction barrel 8, which is beneficial to the uniform dispersion and absorption of CO2, optimizing the movement path of the fluid, enhancing the absorption efficiency of CO2, and reducing the energy consumption during the circulation and treatment processes.

[0030] In summary, through the photobioreactor device composed of structures such as the incubator housing 7, the reaction barrel 8, multiple lantern boxes 9, multiple drainage rings 48, and the drive assembly, the algae can efficiently absorb and utilize CO2, which is beneficial to reducing the emissions of CO2, helping to slow down the emissions of greenhouse gases, and thus having a positive impact on environmental protection and climate change response. Combining wastewater treatment, CO2 absorption, and algae cultivation can reduce the operating cost and improve the resource utilization efficiency, thereby achieving the return on technology investment.

[0031] Specifically, after the algae cultivation is completed, the water and gas supply component is controlled to stop supplying gas to the inside of the reaction barrel 8. Since a sealing plate one 19 is rotatably connected inside the reaction barrel 8, and a plurality of fixed round rods 46 are fixedly installed between the sealing plate one 19 and a sealing plate two 20 fixedly installed inside the culture vessel housing 7, the sealing plate one 19 cannot move up and down. Also, since a mounting frame five 47 rotatably connected to the outside of the reaction barrel 8 is fixedly connected to the culture vessel housing 7, the reaction barrel 8 can rotate inside the culture vessel housing 7. The bottommost lantern box 9 is fixedly installed with a diversion pipe four 11 rotatably connected to the reaction barrel 8, the bottom end of the diversion pipe four 11 is fixedly connected to a diversion pipe five 12, a diversion pipe six 14 and a three-way valve two 13 are both fixedly connected to the vehicle frame 1, the three-way valve two 13 and the diversion pipe five 12 fixedly installed by the three-way valve two 13 cannot rotate, the drainage pipe cannot rotate, and the drainage ring 48 fixedly installed on the reaction barrel 8 does not contact the lantern box 9. Therefore, the rotation of the reaction barrel 8 will not be affected by the diversion pipe four 11 and the drainage pipe. A plurality of metal plates 43 are fixedly installed on the outside of the mounting frame two 22. The plurality of metal plates 43 are all arranged inside an electromagnet 44 rotatably connected to the bottom of the sealing plate one 19. A plurality of connecting side plates 45 are fixedly installed between the outside of the electromagnet 44 and the reaction barrel 8. While controlling the servo motor 25 to work at high power, the electromagnet 44 is controlled to work. The working electromagnet 44 is adsorbed and fixed together with the iron metal plates 43 by magnetic force. The servo motor 25 drives the mounting frame two 22 to rotate. The mounting frame two 22 drives the reaction barrel 8 to rotate under the action of the plurality of metal plates 43, the electromagnet 44 and the plurality of connecting side plates 45. A large stress is generated between the high-speed rotating reaction barrel 8 and the drainage ring 48 and the water. The algae growing on the inner wall of the reaction barrel 8 and the drainage ring 48 fall off, and the rotating reaction barrel 8 makes the solution move to clean the outer wall of the lantern box 9, cleaning the algae growing on the outer wall of the lantern box 9.

[0032] Synchronously control the electric telescopic rod 23 fixedly installed inside the mounting frame two 22 to work and extend. The electric telescopic rod 23 pushes the stirring frame 21 downward to enter the drainage pipe through the diversion pipe three 10. The rotating stirring frame 21 makes the solution inside the drainage pipe move to clean the inner wall of the lantern box 9. After the electric telescopic rod 23 pushes the stirring frame 21 into the diversion pipe four 11, the electric telescopic rod 23 contracts to drive the stirring frame 21 to move upward into the diversion pipe three 10, completing one cleaning work inside the drainage pipe. After the electric telescopic rod 23 has carried out multiple internal cleaning operations on the drainage pipe, control the servo motor 25 to stop working, control the electromagnet 44 to stop working, and control the electric telescopic rod 23 to drive the stirring frame 21 to move up and reset, then the internal cleaning of the reactor composed of the incubator shell 7, the reaction barrel 8 and the drainage pipe can be completed, and most of the algae growing on the inner wall of the reaction barrel 8, multiple drainage rings 48, the inner wall of the lantern box 9 and other positions can be cleaned off. Then control the normally closed end of the second three-way valve 13 to open, control the normally closed end of the first three-way valve 4 to open, and the first diversion pipe 5 fixedly installed at the normally closed end of the first three-way valve 4 is connected to the microalgae recovery system. At this time, the algae solution mixture inside the reaction barrel 8 is discharged along the fourth diversion pipe 11, the fifth diversion pipe 12, the normally closed end of the second three-way valve 13, the sixth diversion pipe 14, the normally closed end of the first three-way valve 4 and the first diversion pipe 5. The algae cultivated with waste water and waste gas are collected, and the algae can be used for processing and producing feed and fertilizer, efficiently utilizing the waste water and waste gas, and further improving the economic benefits.

[0033] Subsequently, control the water and gas supply component to first supply a certain amount of waste water into the reaction barrel 8, and then supply waste gas into the drainage pipe, so that some of the remaining algae inside the reaction barrel 8 continue to grow using the waste water and waste gas.

[0034] Specifically, a second sealing plate 20 is fixedly installed at the top of the inner cavity of the incubator shell 7. A plurality of first exhaust pipes 29 penetrate through the confluence box 27 fixedly installed at the top of the second sealing plate 20. The bottom ends of the first exhaust pipes 29 penetrate through the first sealing plate 19 and are arranged at the top of the inner cavity of the reaction barrel 8. There is a sufficient distance between the bottom end of the first sealing plate 19 and the topmost drainage ring 48, and the splashed solution under the action of aeration and low-speed agitation cannot enter the inside of the first exhaust pipes 29. Moreover, the air guide grooves 30 opened on the outer side of the first exhaust pipes 29 are arranged inside the confluence box 27. The gas inside the reaction barrel 8 enters the confluence box 27 through the first exhaust pipes 29 and the air guide grooves 30. A second exhaust pipe 50 is fixedly installed at the top of the confluence box 27. The second exhaust pipe 50 is connected to the waste gas collection system after passing through the fourth mounting frame 28. The setting of the first exhaust pipes 29 plays a role in relieving pressure inside the reaction barrel 8.

[0035] At the top of the busbar box 27, a fourth mounting bracket 28 is fixedly installed. Inside the fourth mounting bracket 28, a plurality of nuts are fixedly installed. At the bottom of each of the plurality of nuts, a third mounting groove 33 is provided. The plurality of third mounting grooves 33 are all opened at the top of the busbar box 27. The plurality of first mounting grooves 31 provided at the bottom of the plurality of third mounting grooves 33 are all opened at the top of the second sealing plate 20. At the bottom of each of the plurality of first mounting grooves 31, a second mounting groove 32 is provided. The plurality of second mounting grooves 32 are all opened at the top of the first sealing plate 19. An aligned third mounting groove 33, a first mounting groove 31, and a second mounting groove 32 form an installation channel. A first exhaust pipe 29 rotates through a nut and then continues to rotate the first exhaust pipe 29 to move it downward. The bottom end of the first exhaust pipe 29 passes through an installation channel and extends to the top of the inner cavity of the reaction barrel 8; and both the first exhaust pipe 29 and the busbar box 27 are made of high light transmittance glass. Therefore, after the air guide groove 30 enters the inside of the busbar box 27, the rotation operation of the first exhaust pipe 29 is stopped. At this time, the first exhaust pipe 29 only plays a role in guiding air.

[0036] When it is necessary to sample and analyze the algae growing inside the reaction barrel 8, rotate the first exhaust pipe 29 to move the first exhaust pipe 29 downward. After the bottom end of the first exhaust pipe 29 rotates and moves downward for a certain distance, it abuts against the uppermost drainage ring 48. During this process, since the external thread part 34 in the sampling assembly is threadedly installed at the top of the inner cavity of the first exhaust pipe 29, the piston end of the telescopic rod 35 fixedly installed inside the external thread part 34 is fixedly installed with an operating rod 36. At the bottom end of the operating rod 36, two spring telescopic rods 38 are fixedly installed. The outer shells of the two spring telescopic rods 38 both penetrate through the external thread part 34 and are fixedly connected to the external thread part 34. At the bottom end of one of the spring telescopic rods 38, a third sealing plate 39 is fixedly installed. At the bottom end of the other spring telescopic rod 38, a fourth sealing plate 40 is fixedly installed. And at the bottom of both the third sealing plate 39 and the fourth sealing plate 40, a clamping groove 42 is opened. At the bottom of the inner cavity of the first exhaust pipe 29, a limiting ring 41 is fixedly installed. The limiting ring 41 abuts against the limiting ring 41 under the support of the fixedly installed spring telescopic rod 38. The fourth sealing plate 40 is located at the bottom of the inner cavity of the first exhaust pipe 29, and the third sealing plate 39 is located above the fourth sealing plate 40. Therefore, when the first exhaust pipe 29 rotates and moves downward, the first exhaust pipe 29 drives the fourth sealing plate 40 to rotate and move downward. One side of the fourth sealing plate 40 is not sealed. The rotating fourth sealing plate 40 plays a sampling role. A part of the algae and water at the top of the topmost drainage ring 48 enter the inside of the first exhaust pipe 29; When the bottom end of the first exhaust pipe - 29 abuts against the uppermost drainage ring 48, the water inside the first exhaust pipe - 29 is isolated from the outside. Press the operating rod 36 that is slidably installed inside the external thread member 34 to move the operating rod 36 downward. The operating rod 36 pushes the two spring - type telescopic rods 38 downward. Since the fourth sealing plate 40 is limited by the limiting ring 41 and cannot move downward, the spring - type telescopic rod 38 fixedly installed on the fourth sealing plate 40 contracts, and the spring - type telescopic rod 38 fixedly installed on the third sealing plate 39 moves downward. After the third sealing plate 39 moves to the outside of the limiting ring 41, the third sealing plate 39 moves downward and is limited. The third sealing plate 39 and the fourth sealing plate 40 cooperate to seal the bottom of the inner cavity of the first exhaust pipe - 29. Part of the water containing algae is hermetically stored inside the first exhaust pipe - 29 as a sample. Also, because the operating rod 36 and the external thread member 34 are in an interference fit state, the force exerted by the spring - type telescopic rod 38 on the operating rod 36 is not sufficient to move the operating rod 36 upward to reset. Therefore, after releasing the operating rod 36, the relative position between the third sealing plate 39 and the fourth sealing plate 40 does not change, and the bottom of the inner cavity of the first exhaust pipe - 29 is in a sealed state.

[0037] Subsequently, control the exhaust gas collection system to increase the power of pumping air from inside the confluence box 27, so that the air pressure inside the confluence box 27 is lower than the outside. The exhaust gas inside the first exhaust pipe - 29 whose bottom of the inner cavity is sealed by the third sealing plate 39 and the fourth sealing plate 40 is extracted.

[0038] Subsequently, the staff rotates the first exhaust pipe - 29 upward. When the air guide groove 30 opened on the first exhaust pipe - 29 leaves the inside of the confluence box 27, stop operating the first exhaust pipe - 29. At this time, the first exhaust pipe - 29 is no longer connected to the inside of the confluence box 27. The bottom of the inner cavity of the first exhaust pipe - 29 is sealed by the third sealing plate 39 and the fourth sealing plate 40, providing sufficient sampling time for the staff and avoiding exhaust gas leakage to the outside during the process of rotating the first exhaust pipe - 29 to make the air guide groove 30 leave the inside of the confluence box 27; When the air guide groove 30 stays outside the confluence box 27, the staff can obtain samples from inside the first exhaust pipe - 29 through the air guide groove 30 using relatively small sampling devices such as syringes, straws, cotton balls, etc. Since the first exhaust pipe - 29 and the confluence box 27 are made of transparent materials, the sampling can be successfully completed.

[0039] After the sampling is completed, rotate the first exhaust pipe - 29 downward so that the bottom end of the first exhaust pipe - 29 extends to the top of the inner cavity of the reaction barrel 8. The air guide groove 30 opened on the first exhaust pipe - 29 enters the inside of the confluence box 27. Subsequently, pull up the operating rod 36 to reset the third sealing plate 39, so that the inside of the first exhaust pipe - 29 is connected to the inside of the reaction barrel 8 and the confluence box 27.

[0040] In summary, sampling components are provided inside each of the multiple exhaust pipes 29 for relieving pressure inside the reaction barrel 8. During the process of cultivating algae using wastewater and waste gas with a photobioreactor device, algae samples can be obtained multiple times from different positions inside the photobioreactor device with little impact on algae cultivation and extremely low waste gas leakage, facilitating the detection of the cultivation effect of the photobioreactor device.

[0041] Additionally, after one cultivation cycle of the photobioreactor device, separate the exhaust pipe 29 from the nut fixedly installed on the mounting frame 4. Complete the disassembly of the exhaust pipe 29 from the photobioreactor device. For the rubber tube 37 fixedly installed outside the external thread member 34, after limiting the exhaust pipe 29 by holding it, hold the rubber tube 37 and rotate the external thread member 34 to separate the external thread member 34 from the exhaust pipe 29, enabling thorough cleaning of the inside of the exhaust pipe 29 and the sampling component, facilitating the next sampling operation.

[0042] In addition, multiple short rods are fixedly installed between the first three-way valve 4 and the vehicle frame 1, and the first three-way valve 4 cannot move.

[0043] The effect of using this device for the treatment of ammonia nitrogen in propionic acid-containing water bodies by thiamine-coupled microalgae was studied, and the control group did not contain thiamine.

[0044] Chlorella vulgaris ( Chlorella vulgaris and Tetradesmus obliquus Tetradesmus obliquus

[0045] were purchased from the Algae Culture Collection of the Institute of Hydrobiology, Chinese Academy of Sciences. + Artificially simulated livestock and poultry digestion wastewater (COD: 650 mg / L, NH4

[0046] -N: 500 mg / L, TP: 41 mg / L, TN: 550 mg / L) was used; 2 L of distilled water was used to prepare the simulated livestock and poultry digestion wastewater according to the following table. The composition of the simulated wastewater was as follows: 1.326 g of glucose, 3.892 g of NH4Cl, 2 g of EDT, 1.5 g of NaHCO3, 0.372 g of KH2PO4, 0.033 g of NaCl, 0.033 g of MgSO4·7H2O, 0.018 g of FeSO4·7H2O, 0.05 g of CaCl2, 0.032 g of KCl.

[0047] The experimental results are as follows: The algae were cultured separately in simulated livestock and poultry anaerobic digestion wastewater. Regarding the effect of thiamine at different concentrations on the biomass of Chlorella vulgaris, when cultured for 1 - 3 days, there was little difference in the growth of Chlorella vulgaris. A high dose (10 mg / L) inhibited the growth of Chlorella vulgaris, while a low dose (0.001 - 1 mg / L) promoted the growth of Chlorella vulgaris. Chlorella vulgaris had the best growth in wastewater with 0.1 mg / L thiamine added, while the test effect of adding 10 mg / L thiamine was not significant (p > 0.05). After 9 days of culture, the growth of Chlorella vulgaris with low doses (0.01 - 1 mg / L) of thiamine added was better than that of the control group. Among them, the experimental group of Chlorella vulgaris with 0.1 mg / L thiamine added had the best growth condition, with a significant difference from the control group (p < 0.05), and the highest biomass was 1.64*10 7 cells / mL, which was 1.19 times that of the control group. The optimal algal density of adding 0.001 mg / L thiamine was basically the same as that of the control group, with no significant difference from the control group (p > 0.05), while the growth of Chlorella vulgaris with 10 mg / L thiamine added was poor, and the algal density was even lower than that of the control group.

[0048] The change in the algal density of Scenedesmus obliquus during the wastewater treatment process showed that thiamine could promote the growth of Scenedesmus obliquus in wastewater. Among them, the algae with 1 mg / L thiamine added had the best growth condition, with a significant difference from the control group (p < 0.05), and the highest biomass was 1.60*10 7 cells / mL. At the same time, the order of the algal harvest amounts of these 5 groups was: 1 mg / L > 0.1 mg / L > 0.001 mg / L > 10 mg / L > control group.

[0049] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non - restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claimed rights.

Claims

1. A photobioreactor device for microalgae cultivation, comprising a vehicle frame (1), a first mounting frame (2), a controller (3), and a cultivator housing (7), characterized in that: Inside the culture vessel housing (7), a reaction barrel (8) is rotatably arranged. Inside the reaction barrel (8), a plurality of lantern boxes (9) are arranged in sequence from top to bottom. Drainage rings (48) are arranged at the outer tops of the plurality of lantern boxes (9). At the bottom end of the lowermost lantern box (9), a fourth diversion pipe (11) is fixedly installed. A plurality of round holes (49) are formed in the outer side of the fourth diversion pipe (11). At the top of the inner cavity of the reaction barrel (8), a first sealing plate (19) is rotatably connected. The bottom of the fourth diversion pipe (11) is connected to a water and gas supply assembly. At the top of the uppermost lantern box (9), a stirring frame (21) is arranged. At the top of the stirring frame (21), a transmission assembly is arranged. At the top of the inner cavity of the culture vessel housing (7), a second sealing plate (20) is fixedly installed. At the top of the second sealing plate (20), a confluence box (27) is fixedly installed. A plurality of first exhaust pipes (29) penetrate through the confluence box (27). The first exhaust pipes (29) extend to the top of the inner cavity of the reaction barrel (8). Sampling assemblies are inserted inside the plurality of first exhaust pipes (29). The sampling assembly includes an external thread part (34) threadedly connected to the top of the inner cavity of the first exhaust pipe (29), a telescopic rod (35) fixedly installed inside the external thread part (34), an operating rod (36) fixedly installed at the top of the telescopic rod (35), and two spring-type telescopic rods (38) fixedly installed at the bottom end of the operating rod (36). At the bottom end of one of the spring-type telescopic rods (38), a third sealing plate (39) is fixedly installed. At the bottom end of the other spring-type telescopic rod (38), a fourth sealing plate (40) is fixedly installed.

2. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: Two adjacent lantern boxes (9) are fixedly installed together. The drainage ring (48) is fixedly installed inside the reaction barrel (8). At the top of the uppermost lantern box (9), a third diversion pipe (10) is fixedly installed. The top end of the third diversion pipe (10) extends to the top of the uppermost drainage ring (48).

3. A photobioreactor device for microalgae cultivation according to claim 1, characterized in that: The water and gas supply assembly includes a fifth diversion pipe (12) fixedly installed at the bottom end of the fourth diversion pipe (11) and a second three-way valve (13) fixedly installed at the bottom end of the fifth diversion pipe (12). The fourth diversion pipe (11) rotatably penetrates through the bottom of the inner cavity of the reaction barrel (8). The normally closed end of the second three-way valve (13) is fixedly installed with a sixth diversion pipe (14). One end of the sixth diversion pipe (14) extends to the bottom of the vehicle frame (1). The sixth diversion pipe (14) and the second three-way valve (13) are both fixedly connected to the vehicle frame (1). One end of the sixth diversion pipe (14) is fixedly connected to a first three-way valve (4). A plurality of short rods are fixedly installed between the first three-way valve (4) and the vehicle frame (1). The normally open end of the first three-way valve (4) is fixedly installed with a second diversion pipe (6). The normally closed end of the first three-way valve (4) is fixedly installed with a first diversion pipe (5).

4. A photobioreactor device for microalgae cultivation according to claim 3, characterized in that: A check valve (15) is fixedly installed at the normally open end of the three-way valve II (13). A seventh diversion pipeline (16) is fixedly installed on one side of the check valve (15). The top of the seventh diversion pipeline (16) penetrates through the second sealing plate (20) and then a flow detector (17) is fixedly installed. An eighth diversion pipeline (18) is fixedly installed at the air inlet end of the flow detector (17).

5. The photobioreactor device for microalgae cultivation according to claim 1, wherein: The first mounting bracket (2) is fixedly installed on one side of the top of the vehicle frame (1). The controller (3) is fixedly installed on one side of the first mounting bracket (2). The incubator housing (7) is fixedly installed on the top of the vehicle frame (1). A plurality of fifth mounting brackets (47) are rotatably installed on the outer side of the reaction barrel (8), and the fifth mounting brackets (47) are fixedly installed inside the incubator housing (7).

6. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: The transmission assembly includes a second mounting bracket (22) arranged at the top of the stirring frame (21), an electric telescopic rod (23) fixedly installed inside the second mounting bracket (22), and a gear box (24) arranged on the outer side of the second mounting bracket (22). The bottom end of the second mounting bracket (22) penetrates through the first sealing plate (19) and the second sealing plate (20) and is rotatably connected to the first sealing plate (19) and the second sealing plate (20). The piston end of the electric telescopic rod (23) is fixedly installed at the top of the stirring frame (21).

7. A photobioreactor device for microalgae cultivation according to claim 6, characterized in that: Two third mounting brackets (26) are fixedly installed on one side of the first mounting bracket (2). A servo motor (25) is fixedly installed between the two third mounting brackets (26). The output end of the servo motor (25) is fixedly connected to the input end of the gear box (24). The gear box (24) is fixedly installed between the two third mounting brackets (26). The second mounting bracket (22) penetrates through the gear box (24) and is fixedly connected to the gear at the transmission end inside the gear box (24).

8. The photobioreactor device for microalgae cultivation according to claim 6, wherein: A plurality of metal plates (43) are fixedly installed on the outer side of the second mounting bracket (22). An electromagnet (44) is rotatably connected to the bottom of the first sealing plate (19). The metal plates (43) are arranged inside the electromagnet (44). A plurality of connecting side plates (45) are fixedly installed between the outer side of the electromagnet (44) and the reaction barrel (8). A plurality of fixed round rods (46) are fixedly installed between the first sealing plate (19) and the second sealing plate (20).

9. The photobioreactor device for microalgae culture according to claim 1, characterized in that: The operating rod (36) is slidably installed inside the external thread member (34) and is in interference fit with the external thread member (34). A rubber tube (37) is fixedly installed on the outer side of the external thread member (34). The housings of the two spring type telescopic rods (38) penetrate through the external thread member (34). Slots (42) are formed at the bottoms of the third sealing plate (39) and the fourth sealing plate (40). A limit ring (41) is fixedly installed at the bottom of the inner cavity of the first exhaust pipe (29).

10. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: A mounting frame four (28) is fixedly installed at the top of the busbar box (27). A plurality of nuts are fixedly installed inside the mounting frame four (28). The plurality of nuts are respectively threadedly sleeved on the outer sides of a plurality of first exhaust pipes (29). A third mounting groove (33) is provided at the bottom of each of the plurality of nuts. The plurality of third mounting grooves (33) are all opened on the top of the busbar box (27). A first mounting groove (31) is provided at the bottom of each of the plurality of third mounting grooves (33). The plurality of first mounting grooves (31) are all opened on the top of the second sealing plate (20). A second mounting groove (32) is provided at the bottom of each of the plurality of first mounting grooves (31). The plurality of second mounting grooves (32) are all opened on the top of the first sealing plate (19). A gas guiding groove (30) is opened on the outer side of the first exhaust pipe (29). The gas guiding groove (30) is arranged inside the busbar box (27). A second exhaust pipe (50) is fixedly installed at the top of the busbar box (27). The second exhaust pipe (50) penetrates through the mounting frame four (28).

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