A photobioreactor device for cultivating microalgae

Abstract

The present invention discloses a photobioreactor device for cultivating microalgae, which relates to the technical field of biological growth containers. The device comprises a vehicle frame, a mounting frame, a controller and a culture vessel shell. A reaction barrel is rotatably arranged inside the culture vessel shell. A plurality of lantern boxes are sequentially arranged inside the reaction barrel from top to bottom. A drainage ring is provided on the top of the outer sides of the plurality of lantern boxes. A diversion pipe four is fixedly installed at the bottom end of the bottommost lantern box. A plurality of exhaust pipes for relieving pressure inside the reaction barrel are provided inside with sampling components. In the process of cultivating algae using wastewater and waste gas using the photobioreactor device, algae samples can be obtained from different positions inside the photobioreactor device multiple times without affecting the algae cultivation and with minimal waste gas leakage, thereby facilitating the detection of the cultivation effect of the photobioreactor device.

Description

Technical Field

[0001] The invention relates to the technical field of biological growth containers, in particular to a photobioreactor device for cultivating microalgae. Background Art

[0002] Microalgae are generally photosynthetic autotrophs with enormous growth potential. They are producers in ecosystems and a crucial component of the primary productive force in the biosphere. Some microalgae grow rapidly and can absorb large quantities of nutrients such as nitrogen and phosphorus from water, making them a novel bioremediation tool for water pollution control.

[0003] Microalgae are a class of fast-growing photosynthetic microorganisms, either prokaryotic or eukaryotic, typically unicellular or simple multicellular. Microalgae grow autotrophically and fix carbon through photosynthesis, absorbing water and CO2 and converting them into carbohydrates, such as oil and starch, while releasing oxygen.

[0004] Microalgae photosynthesis is a complex biochemical process involving the transport and conversion of substances. Its raw materials, H2O and CO2, are converted into products such as O2, starch, and oil through photosynthesis. While absorbing greenhouse gases, microalgae can also produce economically valuable organic matter, offering potential solutions to environmental and energy issues.

[0005] The microalgae bioreactor is used for the industrial cultivation of marine products "microalgae". In order to facilitate light energy supply, the reactor adopts heat-resistant tempered glass cylinder for lighting and LED light source, which has the effect of low energy consumption and low radiant heat of the light source.

[0006] However, in the application process of existing photobioreactors, although a special sampling channel is set up for sampling, it is easy for waste gas in the photobioreactor to leak during the sampling process, causing waste gas to escape and pollute the environment. In addition, the position of the sampling channel is fixed, resulting in sampling only from one position. As a result, after multiple sampling, the algae growth at the sample location is different from that at other locations, and the obtained samples cannot accurately reflect the growth of algae in different time periods. Summary of the Invention

[0007] The object of the present invention is to provide a photobioreactor device for cultivating microalgae to solve the problems raised in the prior art.

[0008] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a photobioreactor device for cultivating microalgae, comprising a vehicle frame, a mounting frame, a controller and a culture vessel shell, a reaction barrel being rotatably arranged inside the culture vessel shell, a plurality of lantern boxes being arranged in sequence from top to bottom inside the reaction barrel, a plurality of lantern boxes being provided with drainage rings on the top of the outer sides of the lantern boxes, a diversion pipe four being fixedly installed at the bottom end of the bottom lantern box, a plurality of circular holes being provided on the outer sides of the diversion pipe four, a sealing plate one being rotatably connected to the top of the inner cavity of the reaction barrel, a water and gas supply component being connected to the bottom of the diversion pipe four, a stirring rack being provided on the top of the top lantern box, and a A transmission assembly is provided, a sealing plate 2 is fixedly installed on the top of the inner cavity of the incubator shell, a junction box is fixedly installed on the top of the sealing plate 2, a plurality of exhaust pipes 1 are passed through the junction box, the exhaust pipes 1 extend to the top of the inner cavity of the reaction barrel, and sampling assemblies are inserted inside the plurality of exhaust pipes 1, the sampling assembly includes an external threaded part threadedly connected to the top of the inner cavity of the exhaust pipe 1, a telescopic rod fixedly installed inside the external threaded part, an operating rod fixedly installed on the top of the telescopic rod, and two spring-type telescopic rods fixedly installed on the bottom end of the operating rod, one of the spring-type telescopic rods is fixedly installed with a sealing plate 3 on the bottom end, and the other spring-type telescopic rod is fixedly installed with a sealing plate 4 on the bottom end.

[0009] Preferably, two adjacent lantern boxes are fixedly installed together, the drainage ring is fixedly installed inside the reaction barrel, and a diversion pipe three is fixedly installed on the top of the top lantern box, and the top of the diversion pipe three extends to the top of the top drainage ring.

[0010] Preferably, the water vapor supply assembly includes a guide pipe five fixedly installed at the bottom end of the guide pipe four and a three-way valve two fixedly installed at the bottom end of the guide pipe five, the guide pipe four is rotatably penetrated through the bottom of the inner cavity of the reaction barrel, the normally closed end of the three-way valve two is fixedly installed with a guide pipe six, one end of the guide pipe six extends to the bottom of the frame, the guide pipe six and the three-way valve two are both fixedly connected to the frame, one end of the guide pipe six is ​​fixedly connected to the three-way valve one, a plurality of short rods are fixedly installed between the three-way valve one and the frame, the normally open end of the three-way valve one is fixedly installed with the guide pipe two, and the normally closed end of the three-way valve one is fixedly installed with the guide pipe one.

[0011] Preferably, a one-way valve is fixedly installed on the normally open end of the three-way valve 2, a guide pipe 7 is fixedly installed on one side of the one-way valve, a flow detector is fixedly installed on the top of the guide pipe 7 after passing through the sealing plate 2, and a guide pipe 8 is fixedly installed on the air inlet end of the flow detector.

[0012] Preferably, the mounting frame 1 is fixedly installed on one side of the top of the frame, the controller is fixedly installed on one side of the mounting frame 1, the incubator shell is fixedly installed on the top of the frame, and multiple mounting frames 5 are rotatably installed on the outside of the reaction barrel, and the mounting frames 5 are fixedly installed inside the incubator shell.

[0013] Preferably, the transmission assembly includes a mounting frame 2 arranged on the top of the stirring frame, an electric telescopic rod fixedly installed inside the mounting frame 2, and a gear box arranged outside the mounting frame 2. The bottom end of the mounting frame 2 passes through the sealing plate 1 and the sealing plate 2 and is rotatably connected to the sealing plate 1 and the sealing plate 2. The piston end of the electric telescopic rod is fixedly installed on the top of the stirring frame.

[0014] Preferably, two mounting frames three are fixedly installed on one side of the mounting frame one, a servo motor is fixedly installed between the two mounting frames three, the output end of the servo motor is fixedly connected to the input end of the gear box, the gear box is fixedly installed between the two mounting frames three, and the mounting frame two passes through the gear box and is fixedly connected to the gear at the transmission end in the gear box.

[0015] Preferably, a plurality of metal plates are fixedly mounted on the outer side of the second mounting frame, the bottom of the first sealing plate is rotatably connected to an electromagnet, the metal plate is arranged inside the electromagnet, a plurality of connecting side plates are fixedly mounted between the outer side of the electromagnet and the reaction barrel, and a plurality of fixed round rods are fixedly mounted between the first sealing plate and the second sealing plate.

[0016] Preferably, the operating rod is slidably installed inside the external threaded part and has an interference fit with the external threaded part. A rubber tube is fixedly installed on the outside of the external threaded part. The two spring-type telescopic rod shells pass through the external threaded part. The bottoms of the sealing plates three and four are both provided with slots. A limiting ring is fixedly installed on the bottom of the inner cavity of the exhaust pipe one.

[0017] Preferably, a mounting frame four is fixedly installed on the top of the junction box, and a plurality of nuts are fixedly installed inside the mounting frame four, and the plurality of nuts are respectively threadedly sleeved on the outside of the plurality of exhaust pipes one, and the bottoms of the plurality of nuts are provided with mounting grooves three, and the plurality of mounting grooves three are opened at the top of the junction box, and the bottoms of the plurality of mounting grooves three are provided with mounting grooves one, and the plurality of mounting grooves one are opened at the top of the sealing plate two, and the bottoms of the plurality of mounting grooves one are provided with mounting grooves two, and the plurality of mounting grooves two are opened at the top of the sealing plate one, and an air guide groove is opened on the outside of the exhaust pipe one, and the air guide groove is arranged inside the junction box, and the exhaust pipe two is fixedly installed on the top of the junction box, and the exhaust pipe two passes through the mounting frame four.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. In the present application, a plurality of exhaust pipes for relieving pressure inside a reaction barrel are each provided with a sampling assembly. During the process of cultivating algae using wastewater and waste gas in a photobioreactor device, algae samples can be obtained from different positions inside the photobioreactor device multiple times without affecting the algae cultivation and with minimal waste gas leakage, thereby conveniently detecting the cultivation effect of the photobioreactor device.

[0020] 2. In this application, during the aeration process, the solution inside the drainage tube moves upward and enters the reaction barrel. After that, the solution inside the reaction barrel moves downward and enters the drainage tube through the circular hole. Since the aeration is carried out for a long time, the solution enters the drainage tube and then enters the bottom of the inner cavity of the reaction barrel due to aeration, so that the water inside and outside the drainage tube circulates. The circulating solution is changed by multiple drainage rings to change the flow trajectory of the water body. By changing the flow trajectory of the solution inside the reaction barrel, the flash effect of the algae in the reaction barrel is improved, the photosynthesis and biomass yield of the algae are improved, and the carbon fixation rate and mass transfer coefficient of the algae are effectively improved.

[0021] 3. This application provides a photobioreactor device composed of a culture vessel shell, a reaction barrel, multiple lantern boxes, multiple drainage rings, a transmission assembly, and other structures, which enables algae to efficiently absorb and utilize CO2, thereby reducing CO2 emissions and helping to slow down greenhouse gas emissions, thereby having a positive impact on environmental protection and climate change response. Combining wastewater treatment, CO2 absorption, and algae cultivation can reduce operating costs, improve resource utilization efficiency, and thus achieve a return on technology investment. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the present invention;

[0023] Figure 2 Schematic diagram of the partial structure of the shell of the culture vessel of the present invention;

[0024] Figure 3 for Figure 2 A magnified view of the structure at point B;

[0025] Figure 4 Schematic diagram of the partial structure of the reaction barrel of the present invention;

[0026] Figure 5 for Figure 4 A magnified view of the structure at point A;

[0027] Figure 6 for Figure 4 A magnified view of the structure at point C;

[0028] Figure 7 This is a schematic structural diagram of the diversion pipe 7 of the present invention;

[0029] Figure 8This is a structural diagram of the sealing plate 2 of the present invention;

[0030] Figure 9 Schematic diagram of the structure of the mounting frame 4 of the present invention;

[0031] Figure 10 This is a schematic structural diagram of the mounting frame 3 of the present invention;

[0032] Figure 11 This is a partial structural diagram of the second mounting frame of the present invention;

[0033] Figure 12 It is a partial structural schematic diagram of the combiner box of the present invention;

[0034] Figure 13 Schematic diagram of the partial structure of the exhaust pipe 1 of the present invention;

[0035] Figure 14 It is a schematic diagram of the partial structure of the sealing plate three of the present invention.

[0036] Among them: 1. Frame; 2. Mounting frame 1; 3. Controller; 4. Three-way valve 1; 5. Diversion pipe 1; 6. Diversion pipe 2; 7. Incubator shell; 8. Reaction barrel; 9. Lantern box; 10. Diversion pipe 3; 11. Diversion pipe 4; 12. Diversion pipe 5; 13. Three-way valve 2; 14. Diversion pipe 6; 15. One-way valve; 16. Diversion pipe 7; 17. Flow detector; 18. Diversion pipe 8; 19. Sealing plate 1; 20. Sealing plate 2; 21. Stirring frame; 22. Mounting frame 2; 23. Electric telescopic rod; 24. Gear box; 25. Servo Servo motor; 26. Mounting frame three; 27. Combiner box; 28. Mounting frame four; 29. ​​Exhaust pipe one; 30. Air guide groove; 31. Mounting groove one; 32. Mounting groove two; 33. Mounting groove three; 34. External threaded part; 35. Telescopic rod; 36. Operating lever; 37. Rubber tube; 38. Spring-loaded telescopic rod; 39. Sealing plate three; 40. Sealing plate four; 41. Limiting ring; 42. Slot; 43. Metal plate; 44. Electromagnet; 45. Connecting side plate; 46. Fixed round rod; 47. Mounting frame five; 48. Drainage ring; 49. Round hole; 50. Exhaust pipe two. DETAILED DESCRIPTION

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0038] Example: Figures 1-14As shown, the present invention provides a technical solution of a photobioreactor device for cultivating microalgae:

[0039] Specifically, the incubator housing 7, the reaction barrel 8, the lantern box 9, the diversion pipe 7 16, the mounting frame 5 47, the diversion ring 48 and other structures in this application are all made of organic glass with a light transmittance of more than 80%, and have good light transmittance;

[0040] The mounting frame 2 is fixedly installed on one side of the top of the frame 1, and the controller 3 is fixedly installed on one side of the mounting frame 2. The controller 3 is used to control the water and gas supply components and the transmission components to work.

[0041] Specifically, the incubator housing 7 is fixedly mounted on the top of the vehicle frame 1, and a plurality of mounting brackets 5 47 are rotatably mounted on the outside of the reaction barrel 8 provided inside the incubator housing 7, and the mounting brackets 5 47 are fixedly mounted inside the incubator housing 7, so that the vertical position of the reaction barrel 8 inside the incubator housing 7 remains unchanged;

[0042] A plurality of lantern boxes 9 are arranged in sequence from top to bottom inside the reaction barrel 8. Two adjacent lantern boxes 9 are fixedly installed together. The plurality of lantern boxes 9 form a drainage tube. A drainage ring 48 is provided on the top of the outer sides of the plurality of lantern boxes 9. The drainage ring 48 is fixedly installed inside the reaction barrel 8. A diversion pipe three 10 is fixedly installed on the top of the top lantern box 9. The top of the diversion pipe three 10 extends to the top of the top drainage ring 48, so the water flow inside the drainage tube can move to the top of the top drainage ring 48; a diversion pipe four 11 is fixedly installed on the bottom end of the bottom lantern box 9. A plurality of circular holes 49 opened on the outside of the diversion pipe four 11 are all arranged at the bottom of the inner cavity of the reaction barrel 8. The drainage tube is connected to the bottom of the inner cavity of the reaction barrel 8 through the plurality of circular holes 49.

[0043] In the water and gas supply assembly, the diversion pipe 5 12 is fixedly installed at the bottom end of the diversion pipe 4 11. The diversion pipe 6 14 is fixedly installed at the normally closed end of the three-way valve 2 13 fixedly installed at the bottom end of the diversion pipe 5 12. One end of the diversion pipe 6 14 extends to the bottom of the frame 1 and is fixedly connected to the three-way valve 1 4. The diversion pipe 2 6 fixedly installed at the normally open end of the three-way valve 1 4 is connected to the water supply system, which supplies wastewater. The diversion pipe 1 5 fixedly installed at the normally closed end of the three-way valve 1 4 is connected to the microalgae recovery system.

[0044] A one-way valve 15 is fixedly installed at the normally open end of the three-way valve 2 13. The setting of the one-way valve 15 prevents the liquid inside the reaction barrel 8 from flowing out through the normally open end of the three-way valve 2 13. A guide pipe 7 16 is fixedly installed on one side of the one-way valve 15. The top of the guide pipe 7 16 passes through the sealing plate 2 20 and is fixedly installed with a flow detector 17. The guide pipe 8 18 fixedly installed at the air inlet end of the flow detector 17 is used to connect to the exhaust gas supply system. The exhaust gas is the exhaust gas containing a relatively high concentration of CO2.

[0045] When the water supply system is initially operated, algae are added to the wastewater. The water supply system transports a solution containing a predetermined amount of microalgae through diversion pipe 2 6, three-way valve 1 4, diversion pipe 6 14, diversion pipe 5 12, diversion pipe 4 11, and circular hole 49 to the drainage pipe and the interior of the reaction barrel 8. When the level of the solution in the reaction barrel 8 reaches the top of the topmost diversion ring 48 but does not submerge the top of diversion pipe 3 10, the water supply is stopped, the normally closed end of three-way valve 2 13 is controlled to close, and the normally open end of three-way valve 2 13 is controlled to open, so that diversion pipe 6 14 is no longer connected to the interior of the reaction barrel 8. The normally closed end of three-way valve 1 4 is controlled to open, and the interior of three-way valve 1 4 is emptied.

[0046] Subsequently, the exhaust gas supply system is controlled to supply exhaust gas at a certain pressure to the interior of the guide pipe 4 11 through the guide pipe 8 18, the flow detector 17, the guide pipe 7 16, the one-way valve 15, the normally open three-way valve 2 13, and the guide pipe 5 12. The exhaust gas is then rushed into the interior of the drainage pipe formed by the multiple lantern boxes 9 through the guide pipe 4 11. Aeration occurs from bottom to top in the drainage pipe. During the aeration process, part of the solution inside the drainage pipe moves upward and leaves the top of the guide pipe 3 10, and the solution falls to the top of the drainage ring 48 at the bottom.

[0047] Except for the drainage ring 48 at the top, the other drainage rings 48 are all set in the depression of the drainage pipe. There is a certain space between the drainage ring 48 and the drainage pipe to meet the needs of solution flow. The circular hole 49 opened on the outside of the diversion pipe 411 is set at the bottom of the inner cavity of the reaction barrel 8. During the aeration process, the solution in the drainage pipe moves up and enters the reaction barrel 8. After that, the solution in the reaction barrel 8 moves down and enters the drainage pipe through the circular hole 49. Since aeration is carried out for a long time, the solution enters the drainage pipe and then enters the bottom of the inner cavity of the reaction barrel 8 due to aeration, so that the water inside and outside the drainage pipe circulates;

[0048] The circulating solution is changed in its flow trajectory by multiple drainage rings 48. By changing the flow trajectory of the solution inside the reaction barrel 8, the flash effect of the algae in the reaction barrel 8 is improved, the photosynthesis and biomass yield of the algae are improved, and the carbon fixation rate and mass transfer coefficient of the algae are effectively improved.

[0049] In summary, when the exhaust gas moves upward inside the drainage pipe, it drives the solution at the bottom of the inner cavity of the reaction barrel 8 and the bottom of the inner cavity of the drainage pipe to rise inside the drainage pipe, and the solution outside the drainage pipe falls, forming a circulating water body. Due to the structural design of the multiple lantern boxes 9 that make up the drainage pipe and the existence of multiple drainage rings 48 on the outside of the drainage pipe, the solution bends up and down in the longitudinal section. At the same time, light shines on the incubator shell 7. The closer to the central axis of the reaction barrel 8, the smaller the light intensity, which improves the flash effect of the algae and ensures the quality of algae reproduction.

[0050] Specifically, in the process of conveying the exhaust gas into the drainage pipe, the servo motor 25 in the control transmission assembly is operated, two mounting brackets 3 26 are fixedly installed on one side of the mounting bracket 1 2, the gear box 24 and the servo motor 25 are fixedly installed between the two mounting brackets 3 26, and the output end of the servo motor 25 is fixedly connected to the input end of the gear box 24, the mounting bracket 2 22 passes through the sealing plate 1 19 and the sealing plate 2 20 and is rotatably connected to the sealing plate 1 19 and the sealing plate 2 20, the top end of the mounting bracket 22 passes through the gear box 24 and is fixedly connected to the gear at the transmission end in the gear box 24, so after the servo motor 25 is controlled to work at low power, the servo motor 25 outputs a rotational force to the gear box 24, and the rotational force is transmitted to the gear After the box 24 changes direction and speed, it acts on the second mounting frame 22. The rotation of the second mounting frame 22 drives the electric telescopic rod 23 fixed inside to rotate. The piston end of the electric telescopic rod 23 and the outer shell of the electric telescopic rod 23 are in sliding connection. The piston end of the electric telescopic rod 23 cannot rotate relative to each other inside the outer shell of the electric telescopic rod 23. The stirring frame 21 fixedly mounted on the piston end of the electric telescopic rod 23 rotates synchronously with the second mounting frame 22. Since the stirring frame 21 is arranged at the top of the diversion pipe 3 10, the rotating stirring frame 21 breaks up and mixes the exhaust gas and solution that move upward in the diversion pipe 3 10 due to aeration, thereby increasing the contact opportunity between algae and CO2, which helps to improve the growth rate of algae.

[0051] The lantern-like structure of lantern box 9 creates small vortices as the solution flows, increasing the contact area between the CO₂ gas in the exhaust and the solution, thereby improving the efficiency of CO₂ absorption by the algae. Multiple drainage rings 48 positioned outside the drainage tube guide the solution's movement, creating a complex flow structure within reaction barrel 8. This facilitates uniform dispersion and absorption of the CO₂, optimizes the fluid's movement path, enhances CO₂ absorption efficiency, and reduces energy consumption during circulation and treatment.

[0052] In summary, the photobioreactor device composed of the culture vessel shell 7, the reaction barrel 8, the multiple lantern boxes 9, the multiple drainage rings 48, the transmission assembly and other structures enables the algae to efficiently absorb and utilize CO2, which is beneficial to reducing CO2 emissions and helping to slow down greenhouse gas emissions, thereby having a positive impact on environmental protection and climate change response. Combining wastewater treatment, CO2 absorption and algae cultivation can reduce operating costs, improve resource utilization efficiency, and thus achieve a return on technology investment.

[0053] Specifically, after the algae cultivation is completed, the water vapor supply assembly is controlled to stop supplying air to the interior of the reaction barrel 8. Since the reaction barrel 8 is rotatably connected to a sealing plate 19, and a plurality of fixed round rods 46 are fixedly installed between the sealing plate 19 and the sealing plate 20 fixedly installed inside the incubator shell 7, the sealing plate 19 cannot move up and down; and since the mounting frame 5 47 rotatably connected to the outside of the reaction barrel 8 is fixedly connected to the incubator shell 7, the reaction barrel 8 can rotate inside the incubator shell 7; the guide pipe 4 11 fixedly installed on the bottom lantern box 9 is rotatably connected to the reaction barrel 8, and the bottom end of the guide pipe 4 11 is fixedly connected to the guide pipe 5 12, the guide pipe 6 14 and the three-way valve 2 13 are both fixedly connected to the frame 1, the three-way valve 2 13 and the guide pipe 5 12 fixedly installed on the three-way valve 2 13 cannot rotate, the drainage pipe cannot rotate, and the drainage ring 48 fixedly installed on the reaction barrel 8 does not contact the lantern box 9, so the rotation of the reaction barrel 8 will not be affected by the guide pipe 4 11 and the drainage pipe;

[0054] A plurality of metal plates 43 are fixedly installed on the outside of the mounting frame 22. The plurality of metal plates 43 are all arranged inside the electromagnet 44 rotatably connected to the bottom of the sealing plate 19. A plurality of connecting side plates 45 are fixedly installed between the outside of the electromagnet 44 and the reaction barrel 8. The servo motor 25 is controlled to work at high power while controlling the electromagnet 44 to work. The working electromagnet 44 is adsorbed and fixed to the iron metal plate 43 by magnetic force. The servo motor 25 drives the mounting frame 22 to rotate. The mounting frame 22 drives the reaction barrel 8 to rotate under the action of the plurality of metal plates 43, the electromagnet 44 and the plurality of connecting side plates 45. A large stress is generated between the high-speed rotating reaction barrel 8 and the drainage ring 48 and the water. The algae grown on the inner wall of the reaction barrel 8 and the drainage ring 48 fall off, and the rotating reaction barrel 8 causes the solution to move to clean the outer wall of the lantern box 9, thereby cleaning the algae grown on the outer wall of the lantern box 9.

[0055] The electric telescopic rod 23 fixedly installed inside the second mounting frame 22 is synchronously controlled to extend, and the electric telescopic rod 23 pushes the stirring frame 21 to move downward through the diversion pipe 3 10 into the interior of the drainage pipe. The rotating stirring frame 21 causes the solution inside the drainage pipe to move to clean the inner wall of the lantern box 9. After the electric telescopic rod 23 pushes the stirring frame 21 into the interior of the diversion pipe 4 11, the electric telescopic rod 23 contracts and drives the stirring frame 21 to move upward into the interior of the diversion pipe 3 10, completing the cleaning work inside the drainage pipe once.

[0056] After the electric telescopic rod 23 has performed multiple cleaning operations inside the drainage tube, the servo motor 25 is controlled to stop working, the electromagnet 44 is controlled to stop working, and the electric telescopic rod 23 is controlled to drive the stirring frame 21 to move up and reset, thereby completing the cleaning work inside the reactor composed of the culture vessel shell 7 and the drainage tube of the reaction barrel 8, and cleaning most of the algae growing on the inner wall of the reaction barrel 8, multiple drainage rings 48, and the inner wall of the lantern box 9. Then, the normally closed end of the three-way valve 2 13 is controlled to be opened, and the normally closed end of the three-way valve 1 4 is controlled to be opened. The diversion pipe 1 5 fixedly installed at the normally closed end of the three-way valve 1 4 is connected to the microalgae recovery system. At this time, the algae solution mixture inside the reaction barrel 8 is discharged along the diversion pipe 4 11, the diversion pipe 5 12, the normally closed end of the three-way valve 2 13, the diversion pipe 6 14, the normally closed end of the three-way valve 1 4 and the diversion pipe 1 5. The algae cultivated using wastewater and exhaust gas are collected. The algae can be used to process and produce feed and fertilizer, and the wastewater and exhaust gas are efficiently utilized, further improving the economic benefits.

[0057] Subsequently, the water and gas supply component is controlled to first supply a fixed amount of wastewater into the reaction barrel 8 and then supply waste gas into the drainage pipe, so that the remaining algae in the reaction barrel 8 can continue to grow using the wastewater and waste gas.

[0058] Specifically, a sealing plate 20 is fixedly installed on the top of the inner cavity of the culture vessel shell 7, and a plurality of exhaust pipes 29 are passed through the junction box 27 fixedly installed on the top of the sealing plate 20. The bottom end of the exhaust pipe 29 passes through the sealing plate 19 and is arranged at the top of the inner cavity of the reaction barrel 8. The bottom end of the sealing plate 19 is sufficiently far away from the top drainage ring 48. The solution splashed under the action of aeration and low-speed stirring cannot enter the exhaust pipe 29, and the air guide groove 30 opened on the outside of the exhaust pipe 29 is arranged inside the junction box 27. The internal gas of the reaction barrel 8 enters the junction box 27 through the exhaust pipe 29 and the air guide groove 30. An exhaust pipe 250 is fixedly installed on the top of the junction box 27. The exhaust pipe 250 passes through the mounting frame 4 28 and is connected to the exhaust gas collection system. The setting of the exhaust pipe 29 serves to relieve the pressure inside the reaction barrel 8.

[0059] A mounting frame four 28 is fixedly installed on the top of the junction box 27, and a plurality of nuts are fixedly installed inside the mounting frame four 28. A mounting groove three 33 is provided at the bottom of the plurality of nuts. The plurality of mounting grooves three 33 are all opened at the top of the junction box 27. The plurality of mounting grooves one 31 provided at the bottom of the plurality of mounting grooves three 33 are all opened at the top of the sealing plate two 20. The plurality of mounting grooves one 31 are provided at the bottom of the plurality of mounting grooves one 31. The plurality of mounting grooves two 32 are all opened at the top of the sealing plate one 19. An aligned mounting groove three 33, a mounting groove one 31 and a mounting groove two 32 form a mounting channel. After an exhaust pipe one 29 rotates through a nut, the exhaust pipe one 29 is continued to be rotated to move downward. The bottom end of the exhaust pipe one 29 passes through a mounting channel and extends to the top of the inner cavity of the reaction barrel 8; and the exhaust pipe one 29 and the junction box 27 are both made of high-transmittance glass, so the rotation operation of the exhaust pipe one 29 is stopped after the gas guide groove 30 enters the interior of the junction box 27. At this time, the exhaust pipe one 29 only plays a role in guiding gas.

[0060] When it is necessary to sample and analyze the algae grown inside the reaction barrel 8, the exhaust pipe 29 is rotated to move the exhaust pipe 29 downward, and the bottom end of the exhaust pipe 29 is rotated and moved downward for a distance and then abuts against the uppermost drainage ring 48. In this process, since the external threaded part 34 in the sampling assembly is threadedly installed on the top of the inner cavity of the exhaust pipe 29, the piston end of the telescopic rod 35 fixedly installed inside the external threaded part 34 is fixedly installed with an operating rod 36, and the bottom end of the operating rod 36 is fixedly installed with two spring-type telescopic rods 38. The outer shells of the two spring-type telescopic rods 38 both pass through the external threaded part 34 and are fixedly connected to the external threaded part 34. A sealing plate 39 is fixedly installed at the bottom end of one of the spring-type telescopic rods 38, and the other spring-type telescopic rod 38 is fixedly installed at the bottom end. A sealing plate 40 is fixedly mounted on the bottom end of the telescopic rod 38, and a slot 42 is provided at the bottom of both the sealing plate 39 and the sealing plate 40. A limit ring 41 is fixedly mounted on the bottom of the inner cavity of the exhaust pipe 1 29. The limit ring 41 presses against the limit ring 41 under the support of the spring-loaded telescopic rod 38 to which it is fixed. The sealing plate 40 is located at the bottom of the inner cavity of the exhaust pipe 1 29, and the sealing plate 39 is located at the top of the sealing plate 40. Therefore, when the exhaust pipe 1 29 rotates and moves downward, the exhaust pipe 1 29 drives the sealing plate 40 to rotate and move downward. One side of the sealing plate 40 is not sealed, and the rotating sealing plate 40 serves as a sampling function. A portion of the algae and water at the top of the top drainage ring 48 enters the interior of the exhaust pipe 1 29.

[0061] When the bottom end of the exhaust pipe 29 is against the uppermost drainage ring 48, the water inside the exhaust pipe 29 is isolated from the outside, and the operating rod 36 installed in the external threaded part 34 is pressed and slidably to move the operating rod 36 downward. The operating rod 36 pushes the two spring-type telescopic rods 38 downward. Since the sealing plate 40 is limited by the limiting ring 41 and cannot move downward, the spring-type telescopic rod 38 fixedly installed on the sealing plate 40 contracts, and the spring-type telescopic rod 38 fixedly installed on the sealing plate 39 moves downward. After the sealing plate 39 moves to the outside of the limiting ring 41, the sealing The downward movement of plate three 39 is limited, and sealing plate three 39 cooperates with sealing plate four 40 to seal the bottom of the inner cavity of exhaust pipe one 29. Some water containing algae is sealed and stored as a sample inside exhaust pipe one 29; and because the operating rod 36 and the external threaded part 34 are in an interference fit state, the force applied to the operating rod 36 by the spring-type telescopic rod 38 is not enough to move the operating rod 36 upward and reset. Therefore, after releasing the operating rod 36, the relative position between sealing plate three 39 and sealing plate four 40 will not change, and the bottom of the inner cavity of exhaust pipe one 29 is in a sealed state.

[0062] Subsequently, the exhaust gas collection system is controlled to increase the power of extracting air from the inside of the junction box 27, so that the air pressure inside the junction box 27 is lower than that outside, and the exhaust gas inside the exhaust pipe 1 29 whose bottom of the inner cavity is sealed by sealing plate 3 39 and sealing plate 4 40 is extracted.

[0063] Subsequently, the staff rotates exhaust pipe 1 29 upward. When the air guide groove 30 provided in exhaust pipe 1 29 leaves the interior of the combiner box 27, the operation of exhaust pipe 1 29 is stopped. At this time, exhaust pipe 1 29 is no longer connected to the interior of the combiner box 27. The bottom of the inner cavity of exhaust pipe 1 29 is sealed by sealing plate 3 39 and sealing plate 4 40, providing sufficient time for the staff to take samples and preventing exhaust gas from leaking to the outside during the process of rotating exhaust pipe 1 29 to make the air guide groove 30 leave the interior of the combiner box 27.

[0064] When the air guide groove 30 stays outside the junction box 27, the staff can obtain samples from the inside of the exhaust pipe 29 through the air guide groove 30 using a relatively small sampling device such as a needle, straw, or cotton ball. Since the exhaust pipe 29 and the junction box 27 are made of transparent materials, sampling can be completed smoothly.

[0065] When sampling is completed, the exhaust pipe 29 is rotated downward so that the bottom end of the exhaust pipe 29 extends to the top of the inner cavity of the reaction barrel 8, and the air guide groove 30 opened in the exhaust pipe 29 enters the interior of the junction box 27. Then, the operating rod 36 is pulled up to reset the sealing plate 39, so that the interior of the exhaust pipe 29 is connected with the interior of the reaction barrel 8 and the junction box 27.

[0066] In summary, multiple exhaust pipes 29 for relieving pressure inside the reaction barrel 8 are equipped with sampling components. During the process of cultivating algae using wastewater and exhaust gas using the photobioreactor device, algae samples can be obtained from different positions inside the photobioreactor device multiple times without affecting the algae cultivation and with minimal exhaust gas leakage, thereby conveniently detecting the cultivation effect of the photobioreactor device.

[0067] In addition, after one culture of the photobioreactor device is completed, the exhaust pipe 29 is rotated to separate the nut fixed to the mounting frame 28 to complete the disassembly of the exhaust pipe 29 and the photobioreactor device. The rubber tube 37 fixed to the outside of the external threaded part 34 is held to limit the exhaust pipe 29, and then the rubber tube 37 is held to rotate the external threaded part 34 to separate the external threaded part 34 from the exhaust pipe 29. The interior of the exhaust pipe 29 and the sampling component can be thoroughly cleaned to facilitate the next sampling work.

[0068] In addition, a plurality of short rods are fixedly installed between the three-way valve 4 and the frame 1, so that the three-way valve 4 cannot move.

[0069] The device was used to study the effect of thiamine coupled with microalgae in treating ammonia nitrogen in propionic acid-containing water bodies, and the control group did not contain thiamine.

[0070] Chlorella vulgaris ( Chlorella vulgaris and Tetraselena obliquus ( Tetradesmus obliquus ) was purchased from the Algae Culture Herbarium of the Institute of Hydrobiology, Chinese Academy of Sciences.

[0071] Artificial simulated livestock digestion wastewater (COD: 650 mg / L, NH4 + -N: 500 mg / L, TP: 41 mg / L, TN: 550 mg / L); simulated livestock digestion wastewater was prepared with 2 L of distilled water according to the table below. The composition of the simulated wastewater is as follows: 1.326 g glucose, 3.892 g NH4Cl, 2 g EDT, 1.5 g NaHCO3, 0.372 g KH2PO4, 0.033 g NaCl, 0.033 g MgSO4·7H2O, 0.018 g FeSO4·7H2O, 0.05 g CaCl2, and 0.032 g KCl.

[0072] The two algae were inoculated into simulated livestock digestion wastewater containing propionic acid, and thiamine was added at concentrations of 0.001 mg / L, 0.1 mg / L, 1 mg / L, and 10 mg / L, respectively.

[0073] The experimental results are as follows: The algae were cultured in simulated livestock anaerobic digestion wastewater, and different concentrations of thiamine had an effect on the biomass of Chlorella. After 1-3 days of culture, there was little difference in the growth of Chlorella vulgaris. A high dose (10 mg / L) inhibited the growth of Chlorella vulgaris, while a small dose (0.001-1 mg / L) promoted the growth of Chlorella vulgaris. Chlorella vulgaris grew best in wastewater with the addition of 0.1 mg / L thiamine, while the effect of adding 10 mg / L thiamine was not significant (p>0.05). After 9 days of culture, the growth of Chlorella vulgaris with the addition of a small dose (0.01-1 mg / L) of thiamine was better than that of the control group. Among them, the Chlorella vulgaris experimental group with the addition of 0.1 mg / L thiamine had the best growth, which was significantly different from the control group (p<0.05), and the highest biomass was 1.64*10 7 The optimal algal density of the supplemented 0.001 mg / L thiamine group was basically the same as that of the control group, with no significant difference from the control group (p>0.05). However, the growth of Chlorella vulgaris supplemented with 10 mg / L thiamine was poor, and the algal density was even lower than that of the control group.

[0074] The changes in the density of Tetraselmis obliquus during the wastewater treatment process showed that thiamine could promote the growth of Tetraselmis obliquus in wastewater. The algae growth condition was the best when thiamine was added at 1 mg / L, which was significantly different from the control group (p<0.05). The highest biomass was 1.60*10 7 / mL. At the same time, the order of algae harvest of these five groups was: 1 mg / L>0.1 mg / L>0.001 mg / L>10 mg / L>control group.

[0075] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A photobioreactor device for cultivating microalgae, comprising a culture vessel housing (7), characterized in that: A reaction barrel (8) is rotatably provided inside the shell (7) of the culture vessel. A plurality of lantern boxes (9) are sequentially provided inside the reaction barrel (8) from top to bottom. The plurality of lantern boxes (9) form a drainage tube. A drainage ring (48) is provided on the top of the outer sides of the plurality of lantern boxes (9). A diversion pipe four (11) is fixedly installed at the bottom end of the lantern box (9) at the bottom. A plurality of circular holes (49) are provided on the outer side of the diversion pipe four (11). A sealing plate one (19) is rotatably connected to the top of the inner cavity of the reaction barrel (8). The bottom of the diversion pipe four (11) is connected to a water vapor supply component. A stirring rack (21) is provided on the top of the lantern box (9) at the top. A transmission component is provided on the top of the stirring rack (21). A sealing plate two (20) is fixedly installed on the top of the inner cavity of the culture vessel shell (7). A junction box (27) is fixedly installed on the top of the sealing plate 2 (20), and a plurality of exhaust pipes (29) are passed through the junction box (27), and the exhaust pipes (29) extend to the top of the inner cavity of the reaction barrel (8). A sampling assembly is inserted into the interior of the plurality of exhaust pipes (29), and the sampling assembly includes an external threaded part (34) threadedly connected to the top of the inner cavity of the exhaust pipe (29), a telescopic rod (35) fixedly installed inside the external threaded part (34), an operating rod (36) fixedly installed on the top of the telescopic rod (35), and two spring-type telescopic rods (38) fixedly installed on the bottom end of the operating rod (36), wherein a sealing plate 3 (39) is fixedly installed on the bottom end of one of the spring-type telescopic rods (38), and a sealing plate 4 (40) is fixedly installed on the bottom end of the other spring-type telescopic rod (38); A limiting ring (41) is fixedly installed at the bottom of the inner cavity of the exhaust pipe one (29), the sealing plate four (40) is located at the bottom of the inner cavity of the exhaust pipe one (29), and the sealing plate three (39) is located at the top of the sealing plate four (40). The operating rod (36) pushes the two spring-type telescopic rods (38) to move downward, and the sealing plate four (40) is limited by the limiting ring (41) and cannot move downward. After the sealing plate three (39) moves to the outside of the limiting ring (41), the sealing plate three (39) moves downward and is limited. The sealing plate three (39) and the sealing plate four (40) cooperate to seal the bottom of the inner cavity of the exhaust pipe one (29).

2. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: The two adjacent lantern boxes (9) are fixedly installed together, the drainage ring (48) is fixedly installed inside the reaction barrel (8), there is a space between the drainage ring (48) and the drainage pipe, and the top of the top lantern box (9) is fixedly installed with a drainage pipe three (10), and the top of the drainage pipe three (10) extends to the top of the top drainage ring (48).

3. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: It also includes a vehicle frame (1), a mounting frame (2) and a controller (3), wherein the mounting frame (2) is fixedly mounted on one side of the top of the vehicle frame (1), the controller (3) is fixedly mounted on one side of the mounting frame (2), the incubator shell (7) is fixedly mounted on the top of the vehicle frame (1), and a plurality of mounting frames (47) are rotatably mounted on the outer side of the reaction barrel (8), and the mounting frames (47) are fixedly mounted inside the incubator shell (7).

4. The photobioreactor device for microalgae cultivation according to claim 3, characterized in that: The water vapor supply assembly includes a guide pipe five (12) fixedly installed at the bottom end of the guide pipe four (11) and a three-way valve two (13) fixedly installed at the bottom end of the guide pipe five (12), the guide pipe four (11) is rotatably installed in the bottom of the inner cavity of the reaction barrel (8), the normally closed end of the three-way valve two (13) is fixedly installed with a guide pipe six (14), one end of the guide pipe six (14) extends to the bottom of the frame (1), the guide pipe six (14) and the three-way valve two (13) are both fixedly connected to the frame (1), one end of the guide pipe six (14) is fixedly connected to the three-way valve one (4), a plurality of short rods are fixedly installed between the three-way valve one (4) and the frame (1), the normally open end of the three-way valve one (4) is fixedly installed with the guide pipe two (6), and the normally closed end of the three-way valve one (4) is fixedly installed with the guide pipe one (5).

5. The photobioreactor device for microalgae cultivation according to claim 4, characterized in that: A one-way valve (15) is fixedly installed on the normally open end of the three-way valve 2 (13), a guide pipe 7 (16) is fixedly installed on one side of the one-way valve (15), a flow detector (17) is fixedly installed on the top end of the guide pipe 7 (16) after passing through the sealing plate 2 (20), and a guide pipe 8 (18) is fixedly installed on the air inlet end of the flow detector (17).

6. The photobioreactor device for microalgae cultivation according to claim 3, characterized in that: The transmission assembly includes a mounting frame 2 (22) arranged on the top of the stirring frame (21), an electric telescopic rod (23) fixedly installed inside the mounting frame 2 (22), and a gear box (24) arranged outside the mounting frame 2 (22). The bottom end of the mounting frame 2 (22) passes through the sealing plate 1 (19) and the sealing plate 2 (20) and is rotatably connected to the sealing plate 1 (19) and the sealing plate 2 (20). The piston end of the electric telescopic rod (23) is fixedly installed on the top of the stirring frame (21).

7. The photobioreactor device for microalgae cultivation according to claim 6, characterized in that: Two mounting frames three (26) are fixedly mounted on one side of the mounting frame one (2), a servo motor (25) is fixedly mounted between the two mounting frames three (26), an output end of the servo motor (25) is fixedly connected to an input end of a gear box (24), the gear box (24) is fixedly mounted between the two mounting frames three (26), and the mounting frame two (22) passes through the gear box (24) and is fixedly connected to the gear at the transmission end in the gear box (24).

8. The photobioreactor device for microalgae cultivation according to claim 6, characterized in that: A plurality of metal plates (43) are fixedly mounted on the outside of the second mounting frame (22), an electromagnet (44) is rotatably connected to the bottom of the first sealing plate (19), the metal plate (43) is arranged inside the electromagnet (44), a plurality of connecting side plates (45) are fixedly mounted between the outside of the electromagnet (44) and the reaction barrel (8), and a plurality of fixed round rods (46) are fixedly mounted between the first sealing plate (19) and the second sealing plate (20).

9. The photobioreactor device for cultivating microalgae according to claim 1, characterized in that: The operating rod (36) is slidably mounted inside the external threaded part (34) and is interference-fitted with the external threaded part (34). A rubber tube (37) is fixedly mounted on the outside of the external threaded part (34). The outer shells of the two spring-type telescopic rods (38) pass through the external threaded part (34). The bottoms of the sealing plate three (39) and the sealing plate four (40) are both provided with a slot (42).

10. The photobioreactor device for microalgae cultivation according to claim 1, characterized in that: A mounting frame four (28) is fixedly mounted on the top of the junction box (27), and a plurality of nuts are fixedly mounted inside the mounting frame four (28). The plurality of nuts are respectively threadedly sleeved on the outside of the plurality of exhaust pipes one (29), and a mounting groove three (33) is provided at the bottom of the plurality of nuts. The plurality of mounting grooves three (33) are all opened at the top of the junction box (27), and a mounting groove one (31) is provided at the bottom of the plurality of mounting grooves three (33). The plurality of mounting grooves one (31) are all opened at the top of the sealing plate two (20), and a plurality of mounting grooves one (31) are all provided at the bottom of the plurality of mounting grooves two (32). The plurality of mounting grooves two (32) are all opened at the top of the sealing plate one (19), and an air guide groove (30) is provided on the outside of the exhaust pipe one (29), and the air guide groove (30) is set inside the junction box (27). An exhaust pipe two (50) is fixedly mounted on the top of the junction box (27), and the exhaust pipe two (50) passes through the mounting frame four (28).

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