Novel planktonic bacteria sampling head and sampler

Abstract

The invention discloses a novel planktonic bacteria sampling head which comprises a bottom plate, an air exhaust hole is formed in the middle of the bottom plate, a fan is arranged below the air exhaust hole, a mounting seat is arranged above the air exhaust hole, a culture dish is placed on the mounting seat, at least three movable fixed clamping jaws are arranged on the periphery of the mounting seat, and the fixed clamping jaws are arranged on the mounting seat. At least three movable fixed clamping jaws are matched to fix a culture dish, a sampling distribution plate is arranged above the culture dish, the sampling distribution plate is connected with an outer protective shell, the outer protective shell covers the periphery of the mounting seat and is in sealed connection with the bottom plate to form a sampling space, and an air exhaust hole is in through connection with the sampling space; the fan pumps air in the sampling space away from the exhaust hole, and the sampling distribution plate is used for uniformly diffusing external air to the culture dish. The device can adapt to multiple types of culture dishes, and is convenient and reliable to operate.

Description

Technical Field

[0001] The present invention relates to the technical field of samplers, and particularly to a new type of airborne bacteria sampling head and sampler. Background Art

[0002] An airborne bacteria sampler is an instrument specifically used for collecting suspended microorganisms (such as bacteria, fungi, etc.) in the air, and is widely used in clean environment monitoring, medical treatment, pharmaceutical manufacturing, food processing and other fields to ensure that the air quality meets the hygiene or process standards. It mainly captures the microorganisms in the air onto a specific medium (such as a culture dish, filter membrane, etc.) by actively inhaling air, and then analyzes the types and concentrations of microorganisms through cultivation or molecular detection.

[0003] In the prior art, the structure of the sampling head of an airborne bacteria sampler is generally fixed, and the common specification of the culture dish is 90 mm. When the 90 mm culture dish is fixedly used, culture dishes of other sizes cannot be matched with the sampling head for installation, and it is necessary to purchase new equipment or replace the sampling head to perform the sampling operation.

[0004] Moreover, for the existing sampling head structure, its sampling flow rate only meets the standard of 100 L / min. When the flow rate is constant, the sampling time needs to be greatly increased, resulting in low efficiency. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a new type of airborne bacteria sampling head and sampler, which can adapt to and match various types of culture dishes and is convenient and reliable to operate.

[0006] To solve the above technical problem, the present invention provides a new type of airborne bacteria sampling head, including a bottom plate. A suction hole is provided in the middle of the bottom plate. A blower is provided below the suction hole. An installation seat is provided above the suction hole. The installation seat is used for placing a culture dish. At least three movable fixing claws are provided around the installation seat. At least three movable fixing claws cooperate to fix the culture dish. A sampling distribution plate is provided above the culture dish. The sampling distribution plate is connected to an outer protective housing. The outer protective housing covers the periphery of the installation seat and is hermetically connected to the bottom plate to form a sampling space. The suction hole is connected to the sampling space in a through manner;

[0007] The blower draws the air in the sampling space away from the suction hole, and the sampling distribution plate is used for evenly diffusing the external air towards the culture dish.

[0008] Further, at least three cushion blocks are evenly arranged along the circumferential direction at the bottom of the installation seat. The fixing claws are connected to the ends of the cushion blocks through adjusting screws. A spring is also provided on the surface of the adjusting screw between the fixing claw and the end of the cushion block.

[0009] Further, a guiding groove is provided on the mounting seat corresponding to the fixed claw, and the fixed claw is arranged in the groove and can move along the guiding direction of the guiding groove.

[0010] Further, the bottom plate includes a lower base and an upper base. A stepped groove is provided on the surface of the lower base, and an annular stepped portion is provided at the bottom of the outer periphery of the lower base. The upper base is arranged in the stepped groove and is fixedly connected to the lower base by locking screws. A connecting ring is provided on the surface of the upper base, and the outer surface of the connecting ring is threadedly connected to the inner wall of the outer protective housing. A sealing gasket is further provided on the surface of the upper base corresponding to the bottom of the outer protective housing.

[0011] Further, an air inlet is provided in the middle of the outer protective housing. A sunken connecting ring is provided below the air inlet. The sampling distribution plate is fixed to the bottom of the sunken connecting ring, and a dust-proof cover is provided on the air inlet;

[0012] The bottom of the sunken connecting ring extends into the culture dish, and an over-flow gap is provided between the outer periphery of the sunken connecting ring and the inner wall of the culture dish.

[0013] Further, the blower is fixed on the inner bottom surface of the concave bracket, the concave bracket is fixedly connected to the bottom of the bottom plate, the air inlet of the blower extends into the air extraction hole and is hermetically connected through a first sealing ring. A positioning ring is provided on the surface of the blower on the outer periphery of the air inlet, and the positioning ring is abutted against the bottom of the bottom plate.

[0014] Further, a second sealing ring is provided between the lower base and the upper base.

[0015] A sampler includes the sampling head according to any one of the above.

[0016] Further, it includes a sampling box. The sampling head is arranged on the top of the sampling box, and a control unit, a box door and a lifting handle are provided on the surface of the sampling box.

[0017] Advantages of the present invention:

[0018] The culture dish is fixed by the cooperation of three movable fixed claws. Therefore, culture dishes of different sizes can be well fixed, meeting the needs of different sizes of culture dishes and satisfying the customer's choice requirements for different culture dish models. It meets the requirements of flow diversity and culture dish diversity, bringing better choices and experiences to customers.

[0019] When the outer protective housing is disassembled, the sampling distribution plate can be taken away at the same time, making it convenient to take and place the culture dish. And a smaller-sized sampling space is formed by the cooperation of the outer protective housing and the bottom plate. The sampling distribution plate is fixed in a sunken structure and extends into the culture dish. During the air flow process, it can always hit the culture dish and then flow away, with high collection efficiency. Brief Description of the Drawings

[0020] Figure 1 is a schematic diagram of the overall structure of the sampling head of the present invention;

[0021] Figure 2 is the present invention Figure 1 exploded structure diagram;

[0022] Figure 3 is the present invention Figure 1 cross-sectional structure diagram;

[0023] Figure 4 is the sampling air flow diagram of the present invention;

[0024] Figure 5 is the schematic diagram of the mounting seat structure of the present invention;

[0025] Figure 6 is the schematic diagram of the structure of the fixing claw part of the present invention;

[0026] Figure 7 is the schematic diagram of the connection structure between the sampling distribution plate and the outer protective housing of the present invention;

[0027] Figure 8 is the schematic diagram of the sampler structure of the present invention. Detailed Embodiments

[0028] The present invention will be further described below with reference to the drawings and specific embodiments, so that those skilled in the art can better understand the present invention and be able to implement it, but the embodiments cited do not limit the present invention.

[0029] Referring to Figures 1 to 7 as shown, an embodiment of the novel airborne bacteria sampling head of the present invention includes a bottom plate 1, an air extraction hole 2 is provided in the middle of the bottom plate, a fan 3 is provided below the air extraction hole, a mounting seat 4 is provided above the air extraction hole, a culture dish 5 is placed on the mounting seat, at least three movable fixing claws 6 are provided around the mounting seat, the at least three movable fixing claws cooperate to fix the culture dish, a sampling distribution plate 7 is provided above the culture dish, the sampling distribution plate is connected to the outer protective housing 8, the outer protective housing covers the periphery of the mounting seat and is hermetically connected to the bottom plate to form a sampling space 9, and the air extraction hole is connected to the sampling space in a through manner.

[0030] During use, first move the movable fixing claws away from each other to provide a space larger than the diameter of the culture dish, then place the culture dish in the plurality of fixing claws, and then operate the fixing claws to move towards the center. After the plurality of fixing claws contact the culture dish, they interact with each other to limit and fix the culture dish without shaking.

[0031] Specifically, referring to Figure 5 and Figure 6As shown, at least three pads 10 are evenly arranged along the circumferential direction at the bottom of the mounting base. The fixed claw is connected to the end of the pad through an adjusting screw 11. A spring 12 is also arranged on the surface of the adjusting screw between the fixed claw and the end of the pad. The pad can make there be a space gap between the mounting base and the bottom plate when they are fixedly connected, avoiding the mounting base blocking the air extraction hole and forming a flow channel. For the fixed claw, the thickness dimension of the pad meets the requirements of the threaded connection of the adjusting screw. By rotating the adjusting screw, the fixed claw is extruded to move towards the center of the mounting base or the limit on the fixed claw is released, so that the fixed claw can move away from the center of the mounting base. So as to fix or loosen the petri dish.

[0032] During the process of installing the petri dish, if the position of the fixed claw is in the direction close to the center of the mounting base, it will interfere with the placement of the petri dish. Therefore, through the design of the spring, when the adjusting screw is loosened, the deformation and energy storage of the spring are released, and the spring will push the fixed claw to always abut against the screw head of the adjusting screw, that is, the purpose of automatic movement is achieved. After the petri dish is placed in place, operate the adjusting screw corresponding to each fixed claw. The adjusting screw exerts an axial force on the fixed claw, and the fixed claw moves towards the petri dish and compresses the spring at the same time.

[0033] The fixed claw can be directly sleeved on the screw rod of the adjusting screw. This method will cause the fixed claw to rotate along the outer circumference of the screw rod. Therefore, a guiding groove 13 is arranged on the mounting base corresponding to the fixed claw. The fixed claw is arranged in the groove and can move along the guiding direction of the guiding groove. The guiding groove limits the rotation of the fixed claw, and the fixed claw can only move along the axial direction of the screw rod. When the adjusting screw is rotated, the adjusting screw will not drive the fixed claw to rotate.

[0034] After the petri dish is installed in place, the outer protective shell is fixedly installed on the bottom plate. The outer protective shell covers the petri dish. At this time, a space is formed between the outer protective shell and the bottom plate, and this space is the sampling space, and the petri dish is in the sampling space. Then start the fan, as shown in Figure 4 As shown, the fan works to draw the air in the sampling space away from the air extraction hole. Since the air in the sampling space is drawn away, the external air will enter the sampling space through the sampling distribution plate. Specifically, an air inlet 20 is arranged in the middle of the outer protective shell. The external air enters from the air inlet and is evenly dispersed into the sampling space from the sampling distribution plate. The sampling distribution plate is located above the petri dish, and the air extraction hole is located below the mounting base. The external air evenly impacts on the agar surface in the petri dish to achieve the sampling purpose. Then the air flows from the inside of the petri dish to the outside and towards the air extraction hole below the mounting base and is discharged.

[0035] A sunken connecting ring 21 is provided below the above-mentioned air inlet. The sampling distribution plate is fixed to the bottom of the sunken connecting ring. The bottom of the sunken connecting ring extends into the culture dish. An over-flow gap is provided between the outer periphery of the sunken connecting ring and the inner wall of the culture dish, ensuring that the external air can always impact on the culture dish and preventing it from flowing directly away from the side.

[0036] After the collection time is up, the outer protective housing can be disassembled, then the adjusting screw is loosened to release the fixation of the culture dish, and the culture dish is taken away to complete the sampling.

[0037] Refer to Figure 7 As shown, the above-mentioned sampling distribution plate is fixedly connected to the sunken connecting ring, and the sampling distribution plate can be replaced to achieve the purpose of adjusting the sampling flow rate. When the sampling flow rate increases, the sampling time can be greatly shortened, and the purpose of rapid sampling can be achieved through time adjustment.

[0038] The number of airborne bacteria colonies per unit volume of air is expressed in terms of the counting concentration, with the unit being number / m 3 or number / L. The sampling period is 60 seconds. Therefore, when selecting an airborne bacteria sampler to collect the number of colonies per unit volume of air, it is preferable to choose a sampler with a relatively low air flow velocity (in the range of 0.3 - 0.5 m / s) and a relatively large sampling flow rate (in the range of 28.3 - 200 L / min) to ensure that the moisture on the surface of the culture medium is not dried out when the collection is completed. When collecting in a ten-thousand-class and hundred-class environment, the minimum sampling volume requirements are 500 L / time and 1000 L / time respectively. At this time, there will be requirements for the flow rate of the sampler. The larger the flow rate, the less the collection time and the less the moisture consumption on the surface of the culture medium, and the higher the activity rate of the colonies.

[0039] Therefore, the number of colonies in each culture dish is obtained by counting. The average concentration value of airborne bacteria at each measurement point: Average concentration of airborne bacteria (number / m 3 ) = Number of colonies / Sampling volume.

[0040] For example: At a certain measurement point, the sampling volume is 400 L and the number of colonies is 1. The average concentration of airborne bacteria = 1 / 0.4 = 2.5 number / m 3 .

[0041] For example: At a certain measurement point, the sampling volume is 2 m 3 , the number of colonies is 3. The average concentration of airborne bacteria = 3 / 2 = 1.5 number / m 3 .

[0042] After the above calculation, uniformly punch holes in the sampling distribution plate.

[0043] The above-mentioned outer protective housing and the bottom plate can be connected by threads, which is convenient for disassembly. The bottom plate also needs to consider the installation of the sampling head. To ensure that the sampling head can adapt to the matching and practical use of different-sized devices, the bottom plate is designed to include a lower base 14 and an upper base 15. The surface of the lower base is provided with a stepped groove 16, and the bottom of the outer periphery of the lower base is provided with an annular stepped portion 17. The upper base is arranged in the stepped groove and is fixedly connected to the lower base by locking screws. The surface of the upper base is provided with a connecting ring 18, and the outer surface of the connecting ring is threadedly connected to the inner wall of the outer protective housing. The lower base can be designed according to the shape and size of the installation position of the sampling head, while the upper base only needs to be prepared with a unified size, so that the sampling head can be quickly assembled according to different installation environments, reducing the design cost and replacement cost, and having better versatility. A sealing gasket 19 is also provided on the surface of the upper base corresponding to the bottom of the outer protective housing. After the outer protective housing is screwed and fixed, the airtightness of the sampling space is ensured without air leakage. A second sealing ring is provided between the lower base and the upper base to ensure effective connection between the two without air leakage.

[0044] A dust-proof cover 22 is also provided at the air inlet to avoid pollution when not in use.

[0045] The above-mentioned fan is fixed on the inner bottom surface of the concave bracket 23. The concave bracket is fixedly connected to the bottom of the bottom plate. The air inlet 24 of the fan extends into the air extraction hole and is hermetically connected through a first sealing ring. A positioning ring 25 is provided on the surface of the fan outside the air inlet, and the positioning ring is in contact with the bottom of the bottom plate. When the concave bracket is fixedly connected to the bottom plate, it will drive the fan to move towards the bottom plate direction. The air inlet of the fan extends into the air extraction hole and is effectively sealed without air leakage through the first sealing ring. At the same time, after the positioning ring is in contact with the bottom of the bottom plate, it forms an effective limit with the concave bracket, and there will be no problems such as shaking during the operation of the fan.

[0046] Refer to Figure 8 As shown, the present application also discloses a sampler, including the above-mentioned sampling head 111. The sampler further includes a sampling box 26. The sampling head is arranged on the top of the sampling box. The surface of the sampling box is provided with a control unit 27, a box door 28, and a lifting handle 29.

[0047] During use, first open the box door, then operate on the control unit to control the fan to work, and the sampling head performs sampling. The control unit includes components such as a touch screen and a switching power supply, which is convenient for use. After the sampling is completed, close the box door and remove the culture dish. The lifting handle facilitates moving the entire sampler to a different position.

[0048] The above embodiments are only preferred embodiments given to fully illustrate the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of the present invention are all within the protection scope of the present invention.

Claims

1. A novel sampling head for airborne bacteria, characterized in that, It includes a bottom plate. An air extraction hole is provided in the middle of the bottom plate. A blower is provided below the air extraction hole. A mounting seat is provided above the air extraction hole. The mounting seat is used for placing a culture dish. At least three movable fixing claws are provided around the mounting seat. At least three movable fixing claws cooperate to fix the culture dish. A sampling distribution plate is provided above the culture dish. The sampling distribution plate is connected to an outer protective housing. The outer protective housing covers the periphery of the mounting seat and is hermetically connected to the bottom plate to form a sampling space. The air extraction hole is connected to the sampling space in a through manner; The blower sucks the air in the sampling space away from the air extraction hole. The sampling distribution plate is used for evenly diffusing external air towards the culture dish.

2. The novel airborne bacteria sampling head according to claim 1, characterized in that, At least three cushion blocks are evenly arranged along the circumferential direction at the bottom of the mounting seat. The fixing claw is connected to the end of the cushion block through an adjusting screw. A spring is also provided on the surface of the adjusting screw between the fixing claw and the end of the cushion block.

3. The novel airborne microbe sampling head according to claim 2, wherein A guiding groove is provided on the mounting seat corresponding to the fixing claw. The fixing claw is arranged in the groove and can move along the guiding direction of the guiding groove.

4. The novel airborne microbe sampling head according to claim 1, wherein, The bottom plate includes a lower base and an upper base. A stepped groove is provided on the surface of the lower base. An annular stepped portion is provided at the bottom of the outer periphery of the lower base. The upper base is arranged in the stepped groove and is fixedly connected to the lower base through a locking screw. A connecting ring is provided on the surface of the upper base. The outer surface of the connecting ring is threadedly connected to the inner wall of the outer protective housing. A sealing gasket is also provided on the surface of the upper base corresponding to the bottom of the outer protective housing.

5. The novel airborne microbe sampling head according to claim 1, wherein An air inlet is provided in the middle of the outer protective housing. A sunken connecting ring is provided below the air inlet. The sampling distribution plate is fixed to the bottom of the sunken connecting ring. A dust-proof cover is provided on the air inlet; The bottom of the sunken connecting ring extends into the interior of the culture dish. An overflow gap is provided between the outer periphery of the sunken connecting ring and the inner wall of the culture dish.

6. The novel airborne microbe sampling head according to claim 1, characterized in that, The blower is fixed on the inner bottom surface of a concave bracket. The concave bracket is fixedly connected to the bottom of the bottom plate. The air inlet of the blower extends into the air extraction hole and is hermetically connected through a first sealing ring. A positioning ring is provided on the surface of the blower around the air inlet. The positioning ring abuts against the bottom of the bottom plate.

7. The novel airborne microbe sampling head according to claim 1, wherein, A second sealing ring is provided between the lower base and the upper base.

8. A sampler, characterized in that, It includes the sampling head according to any one of claims 1-7.

9. The sampler according to claim 8, wherein It includes a sampling box. The sampling head is provided on the top of the sampling box. A control unit, a box door and a lifting handle are provided on the surface of the sampling box.

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