Diabetes treatment stem cell culture device and method
By using a stratification mechanism in the incubator, the problem of poor stability of the dish in the incubator is solved, and the stable clamping and precise limit of the dish are achieved, ensuring the reliability and safety of cell culture experiments.
Patent Information
- Application Number
- CN202510561334.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-30
- Publication Date
- 2025-07-18
AI Technical Summary
In the prior art, the Petri dishes have poor stability when stacked and stored in the incubator, and are prone to dumping due to vibration or careless operation, resulting in sample contamination and experiment interruption.
A layered mechanism is adopted, including supporting frames, guide rods, support belts, extrusion frames and Velcro components, to achieve accurate limiting and stable clamping of the Petri dish to ensure that the Petri dish does not fall during movement or operation.
It effectively avoids the risk of dumping the Petri dish during use or movement, improves the stability of the culture device, prevents sample contamination and experimental interruption, and improves operational convenience and reliability.
Smart Images
Figure CN120330052A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of stem cell culture for diabetes treatment, and specifically to a device and method for stem cell culture for diabetes treatment. Background Art
[0002] Diabetes is a chronic disease characterized by abnormally elevated blood glucose levels in the body, usually caused by insufficient insulin secretion or abnormal cell response to insulin. The stem cell culture device for diabetes treatment is an advanced medical technology aimed at utilizing the self-renewal and differentiation potential of the patient's own stem cells to guide the cultured stem cells to differentiate into insulin-producing cells for the treatment of diabetes, which can reduce the risk of immune rejection. The culture device mainly consists of an incubator and a rack.
[0003] The prior art usually adopts the method of stacking the same type of culture dishes on the rack. When the bottom culture dish needs to be taken, the operator needs to first firmly hold the stacked culture dishes on the upper layer with one hand to maintain the balance of the overall stacked structure. If the hand shakes, the multiple stacked culture dishes on the upper layer will immediately collapse. In addition, if the incubator experiences severe vibration during the moving process, the stacked culture dish layer inside it will also become unstable and collapse, resulting in the breakage or contamination of the culture dishes, posing a risk of damaging precious cell samples and delaying the experimental process. Summary of the Invention
[0004] Aiming at the problem of poor stability of stacked storage of culture dishes inside the incubator in the prior art, the present invention provides a device and method for stem cell culture for diabetes treatment.
[0005] The technical solution adopted by the present invention to solve its technical problems is: a stem cell culture device for diabetes treatment, including an incubator, a door is arranged on the incubator, two racks are fixedly connected inside the incubator, and symmetrically arranged guide grooves are opened on the racks; A layering mechanism for stably stacking culture dishes is arranged on each of the racks. The layering mechanism includes a support frame, a guide rod is fixedly connected to the bottom end of the support frame, a plurality of support belts are fixedly connected to the support frame, placing grooves are opened on the support belts, extrusion frames are slidably connected to the support belts, one ends of the plurality of extrusion frames are fixedly connected to a connecting rod together, a pulling handle is fixedly connected to the connecting rod, a symmetrically arranged first magic tape is fixedly connected to one of the extrusion frames, and a symmetrically arranged second magic tape is fixedly connected to one of the support belts.
[0006] Specifically, a plurality of symmetrically arranged universal wheels are arranged at the bottom end of the incubator, and a total of four universal wheels are provided.
[0007] Specifically, the specific shape of the support frame is C-shaped, and the bottom end of the support frame abuts against the top end of the rack.
[0008] Specifically, the specific shapes of the guide rod and the guide groove are both set in a T shape. The guide rod is slidably connected inside the guide groove, and the specific shape of the hand pull is set in a C shape.
[0009] Specifically, the first magic tapes are all set as the suede surface, and the second magic tapes are all set as the hook surface.
[0010] The present invention also provides a culture method applicable to a culture device for stem cells for diabetes treatment, including the following steps: Step 1: The entire incubator can be moved to a designated use position through the universal wheels; Step 2: Then open the box door, remove the support frame from the placement rack. The support frame will drive the guide rod to separate from the guide groove, and then place each culture dish into the placement groove on the support belt; Step 3: Then move the connecting rod to one side through the hand pull. The movement of the connecting rod will drive multiple extrusion frames to move together. The movement of the extrusion frames will squeeze the top wall and the bottom wall of the support belt, so that the placement groove shrinks. The shrunk placement groove will clamp the culture dish. After clamping, the first magic tape and the second magic tape will stick together, so as to fix the position of the extrusion frame. At this time, the placement of the culture dish is completed; Step 4: Then reset the support frame to the placement rack, close the box door, and statically culture the treatment stem cells in the internal culture dish through the incubator.
[0011] The beneficial effects of the present invention: The layering mechanism can orderly stack and place the culture dishes, and with the coordinated cooperation among various components, precise limit of each layer of culture dishes can be achieved. When taking out the culture dishes, the operator does not need to support the top-layer culture dish by hand like the traditional method, but only needs to directly pull out the bottom-layer culture dish, effectively avoiding the risk of the top-layer culture dish tipping over due to taking out the bottom-layer culture dish. Even when the incubator shakes violently during the movement process, the layering mechanism can still keep the stacked structure of the culture dishes stable all the time, completely eliminating the concerns of the operator, greatly enhancing the stability of the culture device, providing a reliable guarantee for cell culture experiments, and effectively avoiding problems such as sample contamination or experiment interruption caused by the tipping over of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] The present invention will be further described below with reference to the drawings and embodiments.
[0013] Figure 1 It is the front view provided by the present invention; Figure 2 It is the separation structure diagram of the layering mechanism and the placement rack provided by the present invention; Figure 3 It is the structural schematic diagram when the side wall of the support frame is cut away provided by the present invention; Figure 4 Schematic diagram of the specific structure of the support belt provided by the present invention; Figure 5 Schematic diagram of the specific position structure of the box door provided by the present invention.
[0014] In the figure: 1, incubator; 2, box door; 3, placement rack; 4, guide groove; 5, layering mechanism; 51, support frame; 52, guide rod; 53, support belt; 54, placement groove; 55, extrusion frame; 56, connecting rod; 57, hand pull; 58, first magic tape; 59, second magic tape; 6, universal wheel. Specific embodiments
[0015] In order to make the technical means, creative features, achieved purposes and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0016] As Figures 1 - 5 shown, the present invention provides the following technical solutions: Embodiment 1: A stem cell culture device for diabetes treatment, including an incubator 1, a box door 2 is arranged on the incubator 1, two placement racks 3 are fixedly connected inside the incubator 1, symmetrically arranged guide grooves 4 are opened on the placement racks 3, and a plurality of symmetrically arranged universal wheels 6 are arranged at the bottom end of the incubator 1, and a total of four universal wheels 6 are provided; During use, the entire incubator 1 can be moved to a specified position through the universal wheels 6, and then the box door 2 can be opened, and the culture dish can be placed on the top end of the placement rack 3 inside the incubator 1. After placement, the box door 2 can be closed, and the incubator 1 can be started to adjust the internal environment of the incubator 1 to a degree suitable for the survival and culture of treatment stem cells.
[0017] Embodiment 2: The technical solution of this embodiment different from Embodiment 1 includes: layering mechanisms 5 for stable stacking of culture dishes are arranged on the placement racks 3, the layering mechanism 5 includes a support frame 51, a guide rod 52 is fixedly connected to the bottom end of the support frame 51, a plurality of support belts 53 are fixedly connected to the support frame 51, placement grooves 54 are opened on the support belts 53, extrusion frames 55 are slidably connected to the support belts 53, one ends of the plurality of extrusion frames 55 are commonly fixedly connected to a connecting rod 56, a hand pull 57 is fixedly connected to the connecting rod 56, a symmetrically arranged first magic tape 58 is fixedly connected to one of the extrusion frames 55, a symmetrically arranged second magic tape 59 is fixedly connected to one of the support belts 53, the specific shape of the support frame 51 is C-shaped, the bottom end of the support frame 51 abuts against the top end of the placement rack 3, the specific shapes of the guide rod 52 and the guide groove 4 are both T-shaped, the guide rod 52 is slidably connected inside the guide groove 4, the specific shape of the hand pull 57 is C-shaped, the first magic tapes 58 are all set as suede surfaces, and the second magic tapes 59 are all set as hook surfaces; When in use, the support frame 51 can be removed from the placement frame 3, and the support frame 51 will drive the guide rod 52 to slide outward inside the guide groove 4. When the guide rod 52 is separated from the guide groove 4, the support frame 51 is taken out, and then the square culture dish or the round culture dish can be placed in the placement groove 54 on the support belt 53. The support belt 53 is made of soft cloth, so the shape of the placement groove 54 can be changed according to the different shapes of culture dishes, so that the support belt 53 can support culture dishes of different shapes, and the applicability is excellent. When a round culture dish is placed in one of the placement grooves 54, the support belt 53 can be placed in the placement groove 54. When the culture dish is grown, the other placement slots 54 are all placed as round culture dishes, and the classification is neat and clear. After the placement is completed, the staff stabilizes the support frame 51 with one hand, and then holds the hand pull handle 57 with the other hand, and pulls the hand pull handle 57 to one side. The moving hand pull handle 57 will drive the connected rod 56 to move, and the connected rod 56 will drive multiple squeezing racks 55 to move. The moving squeezing racks 55 will squeeze the top wall and the bottom wall of the support belt 53. At this time, because the support belt 53 is deformed, the placement slot 54 will shrink, and the shrunken placement slot 54 will clamp the internal culture dish, and then the connected rod 56 will be moved. 56 is moved to a suitable position, at which time the multiple placement grooves 54 are all shrunk to clamp the culture dish, at which time the first Velcro 58 on the velvet surface and the second Velcro 59 on the hook surface are adhered to each other, the position of the extrusion frame 55 is fixed, the culture dish is fixed, and then the support frame 51 can be reset to the original position, and the remaining layered mechanisms 5 are operated in the same way, and square culture dishes or culture dishes of other shapes can be placed. After the operation is completed, the box door 2 can be closed, and the therapeutic stem cells in the culture dish can be statically cultured through the incubator 1. When the bottom culture dish needs to be taken out, the bottom culture dish can be directly pulled out to the outside, and the bottom Pulling out the culture dish will not affect the position stability of the remaining culture dishes at all. Moving the incubator 1 or impacting the incubator 1 will not have any impact on the position stability of the culture dishes, effectively avoiding the risk of the top culture dish tipping over due to taking the bottom culture dish. No matter what operation is performed, the culture dish stack always remains stable, completely eliminating the operator's worries. It not only significantly improves the convenience of the retrieval operation, but also greatly enhances the stability of the culture device, providing reliable protection for cell culture experiments, and effectively avoiding problems such as sample contamination or experiment interruption due to the tipping over of the culture dish.
[0018] The present invention also provides a culture method applicable to a diabetes treatment stem cell culture device, comprising the following steps: Step 1: The entire incubator 1 can be moved to a designated use position via the universal wheels 6; Step 2: Open the box door 2, remove the support frame 51 from the placement frame 3, the support frame 51 will drive the guide rod 52 to separate from the guide groove 4, and then place each culture dish into the placement groove 54 on the support belt 53; Step 3: Then, move the connecting rod 56 to one side by pulling the handle 57 by hand. The movement of the connecting rod 56 will drive multiple extrusion frames 55 to move together. The movement of the extrusion frames 55 will extrude the top and bottom walls of the support belt 53, so that the placement groove 54 is reduced. The reduced placement groove 54 will clamp the petri dish. After clamping, the first magic tape 58 and the second magic tape 59 will be pasted together to fix the position of the extrusion frame 55. At this time, the petri dish is placed; Step 4: Then, reset the support frame 51 to the placement rack 3, close the box door 2, and statically culture the therapeutic stem cells in the internal petri dish through the incubator 1.
[0019] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the descriptions in the specification only illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of the present invention claimed is defined by the appended claims and their equivalents.
Claims
1. A stem cell culture device for diabetes treatment, comprising an incubator (1), a door (2) is arranged on the incubator (1), two placement racks (3) are fixedly connected inside the incubator (1), and symmetrically arranged guiding grooves (4) are formed on each of the placement racks (3); It is characterized in that: A layering mechanism (5) for stably stacking culture dishes is arranged on each of the placement racks (3). The layering mechanism (5) includes a support frame (51), a guiding rod (52) is fixedly connected to the bottom end of the support frame (51), a plurality of support belts (53) are fixedly connected to the support frame (51), placement grooves (54) are formed on each of the support belts (53), extrusion frames (55) are slidably connected to each of the support belts (53), one ends of the plurality of extrusion frames (55) are fixedly connected to a connecting rod (56) together, a hand pull (57) is fixedly connected to the connecting rod (56), a symmetrically arranged first magic tape (58) is fixedly connected to one of the extrusion frames (55), and a symmetrically arranged second magic tape (59) is fixedly connected to one of the support belts (53).
2. The stem cell culture device for diabetes treatment according to claim 1, characterized in that: A plurality of symmetrically arranged universal wheels (6) are arranged at the bottom end of the incubator (1), and a total of four universal wheels (6) are provided.
3. The stem cell culture device for diabetes treatment according to claim 1, characterized in that: The specific shape of the support frame (51) is C-shaped, and the bottom end of the support frame (51) abuts against the top end of the placement rack (3).
4. A stem cell culture device for diabetes treatment according to claim 1, characterized in that: The specific shapes of the guiding rod (52) and the guiding groove (4) are both T-shaped, the guiding rod (52) is slidably connected inside the guiding groove (4), and the specific shape of the hand pull (57) is C-shaped.
5. The stem cell culture device for diabetes treatment according to claim 1, wherein: The first magic tapes (58) are all provided as plush surfaces, and the second magic tapes (59) are all provided as hook surfaces.
6. A culture method applicable to the culture device for stem cells for treating diabetes according to any one of claims 1-5, characterized in that, Including the following steps: Step one: The entire incubator (1) can be moved to a designated use position through the universal wheels (6); Step two: Then open the door (2), remove the support frame (51) from the placement rack (3), the support frame (51) will drive the guiding rod (52) to separate from the guiding groove (4), and then place each culture dish into the placement groove (54) on the support belt (53); Step three: Then move the connecting rod (56) to one side through the hand pull (57), the movement of the connecting rod (56) will drive the plurality of extrusion frames (55) to move together, the movement of the extrusion frames (55) will extrude the top wall and the bottom wall of the support belt (53), so that the placement groove (54) is reduced, the reduced placement groove (54) will clamp the culture dish, after clamping is completed, the first magic tape (58) and the second magic tape (59) will be adhered together, so as to fix the position of the extrusion frame (55), and at this time the placement of the culture dish is completed; Step four: Then reset the support frame (51) to the placement rack (3), close the door (2), and statically culture the treatment stem cells in the internal culture dish through the incubator (1).