Microfluidic detection system and detection method

Abstract

The embodiment of the invention discloses a microfluidic detection system and method, the system comprises a microfluidic chip and a detection device, the detection device is provided with a chip mounting groove, a sample introduction module and a liquid inlet driving assembly, and the chip mounting groove is used for placing the microfluidic chip; the micro-fluidic chip comprises a sample inlet, a reagent groove and a reaction cavity, the reagent groove comprises a detection reagent groove for placing an antibody solution reagent bag and a color developing reagent groove for placing a color developing solution reagent bag; the sample inlet is communicated with the reagent tank and the reaction cavity and is used for detecting a sample and feeding a reagent in the reagent tank and reacting in the reaction cavity; the sample injection module comprises a puncture assembly and a rotating assembly, the bottom of the puncture assembly is located above the reagent tank, and the rotating assembly is used for controlling the puncture assembly to move downwards to puncture a reagent bag in the reagent tank; the liquid inlet driving assembly is communicated with a chip outlet of the micro-fluidic chip and is used for controlling liquid in the micro-fluidic chip to flow. According to the embodiment of the invention, health detection can be quickly realized without professional skills.

Description

Technical Field

[0001] The present application relates to the field of in vitro detection technology, and particularly to a microfluidic detection system and a detection method. Background Art

[0002] With the development of society, people's attention to health has become increasingly high. The increasing demand for home health detection and the expanding demand for home detection have made the detection of POCT (point-of-care testing) one of the main development directions in the detection field in recent years. POCT refers to the detection method of sampling on-site and obtaining the detection result on-site, and quickly obtaining the target analyte by using a portable and compact analytical instrument and supporting reagents.

[0003] Currently, home detection devices have not significantly appeared in the public eye. There are similar rapid biochemical detection devices mainly in hospital laboratories or clinical laboratories, but these detection devices have many problems, such as complex operation, requiring professional skills to operate, and long detection time, etc.

[0004] Therefore, how to provide a detection system that can perform health detection conveniently and quickly is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] The embodiments of the present specification provide a microfluidic detection system and a detection method, which can realize simple and rapid health detection and improve the efficiency of health detection.

[0006] On the one hand, the embodiments of the present specification provide a microfluidic detection system, and the microfluidic detection system includes: a microfluidic chip and a detection device. A chip installation slot, a sample injection module, and a liquid inlet driving component are provided in the detection device; the chip installation slot is used to place the microfluidic chip; the microfluidic chip includes: a sample injection port, a reagent tank, and a reaction chamber;

[0007] The reagent tank includes a detection reagent tank and a chromogenic reagent tank. The detection reagent tank is used to place an antibody solution reagent pack, and the chromogenic reagent tank is used to place a chromogenic solution reagent pack; the reagent in the antibody solution reagent pack is used to perform an immunological binding reaction with the detection sample, and the chromogenic solution reagent pack is used to perform a chromogenic reaction with the liquid after the immunological binding reaction;

[0008] The sample injection port is communicated with both the reagent tank and the reaction chamber, and is used for the detection sample and the reagent in the reagent tank to flow into the reaction chamber to react after sample injection;

[0009] The sample injection module includes a puncture component and a rotation component. The bottom of the puncture component is located above the reagent tank, and the rotation component is used to control the puncture component to move downward so that the puncture component pierces the reagent pack in the reagent tank;

[0010] The liquid inlet driving assembly is communicated with the chip outlet of the microfluidic chip and is used to control the liquid flow in the microfluidic chip.

[0011] Preferably, the number of the puncture assemblies is the same as that of the reagent grooves, each of the puncture assemblies correspondingly corresponds to a corresponding reagent groove, and the rotation assembly is used to respectively control the downward movement of each of the puncture assemblies through rotation to puncture the reagent packets in the corresponding reagent grooves.

[0012] Preferably, the detection device further includes a sterilization and disinfection assembly, and the sterilization and disinfection assembly is arranged between the puncture assembly and the reagent groove of the microfluidic chip and is used to sterilize and disinfect the puncture assembly.

[0013] Preferably, the detection device further includes a vibration assembly, and the vibration assembly is arranged above the reaction chamber and is used to mix the liquid in the reaction chamber.

[0014] Preferably, the reagent groove further includes a detergent reagent groove, and the detergent reagent groove is used to place a washing liquid reagent packet. The antibody liquid reagent packet includes a labeled antibody and an immunomagnet. The labeled antibody is used to perform an immunobinding reaction with the detection sample, and the immunomagnet is used to adsorb specific proteins in the liquid after the immunobinding reaction.

[0015] The detection device further includes an electromagnetic assembly, and the electromagnetic assembly is arranged below the reaction chamber of the microfluidic chip and is used to adsorb the immunomagnet. After the labeled antibody performs an immunobinding reaction with the detection sample, the puncture assembly punctures the washing liquid reagent packet, and the liquid inlet driving assembly pumps the reagent in the washing liquid reagent packet into the reaction chamber to wash the immunomagnet adsorbed by the electromagnetic assembly.

[0016] Preferably, the microfluidic chip further includes a buffer area, and the buffer area is arranged between the reaction chamber and the chip outlet. The buffer area is communicated with both the reaction chamber and the chip outlet, and the buffer area is used to prevent the immunomagnet from being flushed into the chip outlet when the liquid in the microfluidic chip flows.

[0017] Preferably, the reagent groove further includes a termination liquid reagent groove, and the termination liquid reagent groove is used to place a termination liquid reagent packet, and the termination liquid reagent packet is used to terminate the color reaction.

[0018] Preferably, the microfluidic chip further includes a detection chamber, and the detection chamber is arranged at the chip outlet end of the microfluidic chip. The detection chamber is communicated with the reaction chamber, and the detection chamber is used to receive the post-reaction liquid discharged from the reaction chamber after the detection is completed.

[0019] The detection device further includes: a detection result reading component, which includes a photoelectric sensing emission component and a photoelectric sensing receiving component. The photoelectric sensing emission component is arranged below the detection chamber, and the photoelectric sensing receiving component is arranged above the detection chamber. The photoelectric sensing emission component is used to emit a light beam into the detection chamber after the detection is completed. The photoelectric sensing receiving component is used to receive the light beam passing through the reacted liquid in the detection chamber and display different voltage values based on the received light beam to obtain the detection result.

[0020] Preferably, the detection device further includes: a waste liquid collection component, which is communicated with the liquid inlet driving component so that the liquid inlet driving component discharges the waste liquid generated by the detection into the waste liquid collection component.

[0021] Preferably, the detection device further includes a control component, which is communicatively connected to each component in the detection device. A working program is set in the control component, and the working program is used to control the working processes and working states of each component in the detection device.

[0022] Preferably, the microfluidic detection system further includes a detection data application program, which is used to receive and display the detection result and display the corresponding health status analysis and guidance information based on the detection result.

[0023] On the other hand, an embodiment of the present specification provides a microfluidic detection method, which is applied to the above microfluidic detection system. The detection method includes:

[0024] Collect a detection sample and place the collected detection sample into the sample inlet of the microfluidic chip in the microfluidic detection system;

[0025] Install the microfluidic chip into the chip installation slot of the detection device in the microfluidic detection system, rotate the rotation component in the detection device to the first indication position, and use the rotation component to control the puncture component in the detection device to puncture the antibody liquid reagent package in the detection reagent slot of the microfluidic chip so that the reagent in the antibody liquid reagent package flows into the sample inlet;

[0026] Turn on the liquid inlet driving component in the detection device, and use the liquid inlet driving component to suck the detection sample in the sample inlet and the reagent in the antibody liquid reagent package into the reaction chamber in the microfluidic chip, so that the reagent in the antibody liquid reagent package reacts immunologically with the detection sample in the reaction chamber;

[0027] After a first preset time, rotate the rotating component to a second indicated position, and use the rotating component to control the puncturing component to puncture the color-developing liquid reagent pack in the color-developing reagent tank of the microfluidic chip;

[0028] Turn on the liquid inlet driving component in the detection device, and use the liquid inlet driving component to pump the reagent in the color-developing liquid reagent pack into the reaction chamber, so that the reagent in the color-developing liquid reagent pack undergoes a color reaction with the liquid after the immune binding reaction in the reaction chamber;

[0029] After a second preset time, obtain a detection result based on the liquid after the reaction.

[0030] The microfluidic detection system and detection method provided by the embodiments of this specification have the following technical effects:

[0031] The microfluidic detection system provided by the embodiments of this specification integrates the reagents used for detection into reagent packs. When in use, the reagent packs are directly placed in the reagent tanks of the microfluidic chip, which is convenient for transportation and carrying. At the same time, by using the microfluidic chip and the detection device in combination, only need to rotate the rotating component in the detection device and control the puncturing component to puncture the corresponding reagent pack, then the specified reagent in the microfluidic chip can be released, and then through the liquid inlet driving component, the reagent in the microfluidic chip can be automatically pumped into the reaction chamber to undergo corresponding reactions. The whole process is simple to operate, does not require professional technology, has a wider range of application scenarios, improves the efficiency of health detection, and realizes the function of rapid home detection.

[0032] Referring to the following description and drawings, specific embodiments of the present invention are disclosed in detail, indicating the ways in which the principles of the present invention can be adopted. It should be understood that the embodiments of the present invention are not limited in scope thereby. Within the spirit and terms of the appended claims, the embodiments of the present invention include many changes, modifications, and equivalents. Features described and / or illustrated for one embodiment can be used in the same or similar way in one or more other embodiments, combined with features in other embodiments, or replace features in other embodiments. Description of the Drawings

[0033] To more clearly illustrate the technical solutions and advantages in the embodiments of this specification or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of this specification. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

[0034] Figure 1 It is a schematic structural diagram of a microfluidic detection system provided by an embodiment of this specification;

[0035] Figure 2 It is a schematic diagram of a partial structure of a detection device provided by an embodiment of this specification;

[0036] Figure 3 It is an exploded schematic diagram of a microfluidic chip provided by an embodiment of this specification;

[0037] Figure 4 It is a schematic diagram of the structure of a microfluidic chip provided by an embodiment of this specification;

[0038] Figure 5 It is a schematic diagram of a partial structure of a detection device provided by another embodiment of this specification;

[0039] Figure 6 It is a schematic diagram of a partial structure of a puncture assembly provided by an embodiment of this specification;

[0040] Figure 7 It is a schematic diagram of the structure of a rotation assembly provided by an embodiment of this specification;

[0041] Figure 8 It is a schematic diagram of the structure of a rotation assembly provided by another embodiment of this specification;

[0042] Figure 9 It is a schematic cross-sectional structure diagram of a puncture assembly and a rotation assembly provided by an embodiment of this specification;

[0043] Figure 10 It is a schematic diagram of the positional relationship structure between a detection device and a microfluidic chip provided by another embodiment of this specification;

[0044] Figure 11 It is a schematic flowchart of a microfluidic detection method in an embodiment of this specification.

[0045] Explanation of reference numerals:

[0046] 1. Microfluidic chip; 2. Detection device; 3. Display screen; 4. Handle of waste liquid collection assembly; 5. Fixed plate; 6. Support plate;

[0047] 1-1. Sampling port; 1-2. Reagent tank; 1-3. Reaction chamber; 1-4. Buffer zone; 1-5. Detection chamber; 1-6. Chip outlet;

[0048] 2-1. Detection device housing; 2-2. Base of detection assembly; 2-3. Chip installation slot; 2-4. Rotation assembly; 2-5. Vibration assembly; 2-6. Liquid inlet driving assembly; 2-7. Waste liquid collection assembly; 2-8. Detection result reading assembly; 2-9. Puncture assembly; 2-10. Sterilization and disinfection assembly; 2-11. Electromagnetic assembly;

[0049] 2-4-1, Indicator sign; 2-4-2, Gear card board; 2-4-3, Puncture needle opening

[0050] 2-8-1, Photoelectric sensing receiving component; 2-8-2, Photoelectric sensing transmitting component

[0051] 2-9-1, Rotating motor; 2-9-2, Support column; 2-9-3, Spring; 2-9-4, Puncture needle Detailed implementation manners

[0052] Next, the technical solutions in the embodiments of this specification will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of this specification. Obviously, the described embodiments are only a part of the embodiments of this specification, rather than all the embodiments. Based on the embodiments in this specification, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of this specification.

[0053] It should be noted that the terms "first", "second", etc. in the description and claims of the embodiments of this specification and the above-mentioned drawings are used to distinguish similar objects, and do not necessarily need to be used to describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances so that the embodiments of this specification described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or server that includes a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products, or devices.

[0054] In the embodiments of this specification, unless otherwise stated, the orientation terms such as "upper", "lower", "top", and "bottom" are usually in the direction shown in the drawings, or in the vertical, perpendicular, or gravitational direction of the component itself; similarly, for the sake of easy understanding and description, "inner" and "outer" refer to the inner and outer of the contour of each component itself, but the above orientation terms do not limit the embodiments of this specification.

[0055] See Figures 1 - 10 , the embodiments of this specification can provide a microfluidic detection system. This microfluidic detection system has a simple structure, is convenient to carry, and is easy to operate, and can be applied to home health detection.

[0056] Such as Figure 1As shown, the microfluidic detection system provided in an embodiment of this specification may include a microfluidic chip 1 and a detection device 2. Among them, the microfluidic chip can be understood as a new technology developed on the basis of capillary electrophoresis. Different functional units such as microchannels, microreactors, and microdetectors are integrated through microfabrication methods, and a biochemical reaction is miniaturized onto a chip with a side length of a few square centimeters. In the embodiments of this specification, applying the microfluidic chip to the microfluidic health detection system can achieve the purpose of rapid home immunity detection. The detection device 2 can be understood as a device that cooperates with the microfluidic chip 1 to perform rapid and simple operations on the microfluidic chip 1 and obtain detection results.

[0057] As Figure 2 shown, in some embodiments, the detection device 2 may include a detection device housing 2-1, a detection component base 2-2, and a chip installation slot 2-3. Among them, the chip installation slot 2-3 is matched with the microfluidic chip 1 and can be used to install the microfluidic chip 1. By using the various components in the detection device 2 to perform relevant operations, rapid detection can be achieved using the reagents in the microfluidic chip 1.

[0058] As Figure 4 shown, in some embodiments, the microfluidic chip 1 may include a sample injection port 1-1, a reagent tank 1-2, and a reaction chamber 1-3. Among them, the sample injection port 1-1 is connected to the reagent tank 1-2 and is used for injecting the test sample and the reagent in the reagent tank 1-2. The reaction chamber 1-3 is connected to the sample injection port 1-1, enabling the liquid in the sample injection port 1-1 to flow into the reaction chamber 1-3, where corresponding reactions occur. As Figures 3 - 4 shown, there are generally multiple reagent tanks 1-2, and different reagent tanks 1-2 can be used to place corresponding reagent packs. In some embodiments of this specification, the reagent tank 1-2 may include a detection reagent tank and a color development reagent tank, and marks can be made on the reagent tank 1-2, such as: No. 1, No. 2. The No. 1 reagent tank can be used as the detection reagent tank, and the No. 2 reagent tank can be used as the color development reagent tank. Or, the name can be directly marked next to the reagent tank 1-2 for easy distinction. Among them, the detection reagent tank can be used to place the antibody solution reagent pack, and the reagent in the antibody solution reagent pack is mainly used to perform an immune binding reaction with the test sample; the color development reagent tank can be used to place the color development solution reagent pack, and the reagent in the color development solution reagent pack is mainly used to perform a color development reaction with the liquid after the immune binding reaction. Based on the color of the liquid after the color development reaction, the concentration of the liquid after the immune binding reaction can be obtained, and then the corresponding detection result can be obtained.

[0059] In addition, as Figure 3As shown, in some embodiments of this specification, fixing plates 5 and support plates 6 can be arranged above and below the microfluidic chip 1. Among them, the fixing plate 5 can be used to fixedly connect the microfluidic chip 1 to the detection device 2 to prevent the microfluidic chip 1 from sliding in the chip installation groove 2-3. The support plate 6 can be fixedly connected to the bottom surface of the microfluidic chip 1 to support the microfluidic chip 1. Generally, the microfluidic chip 1 is relatively soft, and the microfluidic chip 1 can be fixedly supported by the fixing plate 5 and the support plate 6. The materials of the fixing plate 5 and the support plate 6 can be selected according to actual needs, such as: glass, which is not specifically limited in the embodiments of this specification. In addition, the fixing plate 5 and the support plate 6 can also enclose the upper and lower surfaces of the microfluidic chip 1, so that reagents and detection samples can be placed in the microfluidic chip 1.

[0060] The microfluidic chip 1 can use PDMS (Polydimethylsiloxane) as the main raw material. Of course, other materials can also be selected according to actual needs, such as: plastics, glass, PMMA (Polymethyl Methacrylate) organic substances, etc. The specific material of the microfluidic chip is not limited in the embodiments of this specification.

[0061] Such as Figures 5 - 8 As shown, in some embodiments, the detection device 2 can include a sampling module 2-4 and a liquid inlet driving component 2-6. Among them, the sampling module 2-4 can include a puncture component 2-9 and a rotation component 2-11. As Figure 6 shown, the bottom of the puncture component 2-9 is located directly above the reagent tank 1-2. As Figure 9 shown, the puncture component 2-9 and the rotation component 2-11 cooperate. The rotation of the rotation component 2-11 can be used to control the downward movement of the puncture component 2-9. When the puncture component 2-9 moves downward, it will pierce the reagent pack in the reagent tank 1-2 below it, so that the reagent in the reagent pack flows into the sampling port 1-1. As Figure 5 shown, the liquid inlet driving component 2-6 is connected to the chip outlet 1-6 of the microfluidic chip 1 and can be used to drive the liquid in the microfluidic chip 1 to flow in the microfluidic chip 1, such as: pumping the liquid in the sampling port 1-1 into the reaction chamber 1-3.

[0062] In addition, as Figure 5 and Figure 7 shown, an indication mark 2-11-1 and a gear retaining plate 2-11-2 can be arranged on the rotation component 2-11. As Figure 1As shown, at the position on the detection device housing 2-1 of the detection device 2 corresponding to the rotating assembly 2-11, there may be provided markings, such as: 1, 2, 3, 4. This marking corresponds to the reagent tank 1-2 in the microfluidic chip 1. For example: 1 corresponds to the No. 1 reagent tank, and 2 corresponds to the No. 2 reagent tank. The indicating mark 2-11-1 on the rotating assembly 2-11 can be aligned with the marking provided on the detection device housing 2-1 to control the puncturing assembly 2-9 to pierce the reagent package in the corresponding reagent tank, so as to accurately release the reagent in the reagent package and facilitate the detection operation. The gear retaining plate 2-11-2 is mainly used to fix the rotating assembly 2-11 after the indicating mark 2-11-1 is aligned with the marking on the detection device housing 2-1, so that the rotating assembly 2-11 can control the puncturing assembly 2-9 to pierce the reagent package in the corresponding reagent tank.

[0063] For example: When performing a health check, the test sample can be collected into the sample inlet 1-1 in the microfluidic chip 1, and then the rotating assembly 2-11 is rotated so that the indicating mark 2-11-1 on the rotating assembly 2-11 is aligned with the marking on the detection device housing 2-1 indicating the detection reagent tank, such as: the 1st position. Rotating the rotating assembly 2-11 to the corresponding position can control the puncturing assembly 2-9 to move downward and pierce the antibody liquid reagent package in the detection reagent tank below it. After the reagent in the antibody liquid reagent package flows into the sample inlet 1-1, the liquid inlet driving assembly 2-6 can be activated. The liquid inlet driving assembly 2-6 can suck the reagent and the test sample in the sample inlet 1-1 into the reaction chamber 1-3 connected to the sample inlet 1-1 together, so that the reagent and the test sample can fully perform an immunological binding reaction in the reaction chamber 1-3. After the immunological binding reaction is completed, through the same operation, the rotating assembly 2-11 can be rotated to align with the corresponding marking of the color developing reagent tank, such as: the 2nd position. The rotating assembly 2-11 can control the corresponding puncturing assembly 2-9 to move downward and pierce the color developing liquid reagent package in the color developing reagent tank below it. The color developing liquid reagent in the color developing liquid reagent package flows into the sample inlet 1-1. Then the liquid inlet driving assembly 2-6 is activated, and the liquid inlet driving assembly 2-6 can suck the color developing liquid reagent in the sample inlet 1-1 into the reaction chamber 1-3, so that the reagent in the color developing liquid reagent package can react with the liquid of the immunological binding reaction to develop a color. The concentration of the liquid after the immunological binding reaction can be analyzed based on the color after the color development reaction, and then the corresponding test result can be obtained.

[0064] Among them, the driving principle of the liquid inlet driving component 2-6 can be selected according to actual needs. For example, electrophoresis can be used to control liquid driving, or capillary force can be used to control liquid driving, or a precision pressure pump can be used for driving control, or negative pressure air dissolution method can be used for driving control, etc. The driving principle and specific structure of the liquid inlet driving component 2-6 are not limited in the embodiments of this specification. The working duration of the liquid inlet driving component 2-6 can be controlled according to the flow rate of the liquid in the microfluidic chip 1 and the size of the microfluidic chip 1, such as the distance from the sample inlet 1-1 to the reaction chamber 1-3, so that the liquid in the sample inlet 1-1 can flow into the reaction chamber 1-3 and will not directly flow out of the chip outlet 1-6.

[0065] The microfluidic chip 1 in the embodiments of this specification can be a disposable microfluidic chip or a reusable microfluidic chip. For example, if the microfluidic chip 1 is disposable, after the detection is completed, the microfluidic chip 1 can be directly taken out of the detection device 1 and placed in a designated recycling location. Or after the detection is completed, the microfluidic chip 1 can be taken out of the detection device 1, the used reagent pack in the reagent tank 1-2 of the microfluidic chip 1 can be taken out and placed in a designated location, the microfluidic chip 1 can be rinsed and dried, and then a new reagent pack can be placed in the reagent tank 1-2 for the next use, realizing the multiple reuse of the microfluidic chip 1, which can improve the utilization rate of the microfluidic chip 1.

[0066] The microfluidic detection system provided by the embodiments of this specification integrates the reagents used for detection into reagent packs, and directly places the reagent packs in the reagent tanks of the microfluidic chip 1 during use, which is convenient for transportation and carrying. At the same time, by using the microfluidic chip 1 and the detection device 2 in combination, only by rotating the rotating component 2-11 in the detection device 2 and controlling the piercing component 2-9 to pierce the corresponding reagent pack, the designated reagent in the microfluidic chip 1 can be released, and then the liquid in the microfluidic chip 1 can be automatically pumped into the reaction chamber 1-3 through the liquid inlet driving component 2-6 to cause corresponding reactions. The whole process is simple to operate, does not require professional technology, has a wider range of applicable scenarios, improves the efficiency of health detection, and realizes the function of rapid home detection.

[0067] Such as Figure 5 、 Figure 10As shown, in some embodiments of this specification, the detection device 2 may further include a vibration component 2-5. The vibration component 2-5 may be disposed directly above the reaction chamber 1-3 of the microfluidic chip 1. When the reagent in the microfluidic chip 1 flows into the reaction chamber 1-3 and relevant chemical reactions need to occur, the vibration component 2-5 may be activated. Through the vibration of the vibration component 2-5, the liquid in the reaction chamber 1-3 can be uniformly and fully mixed, improving the reaction speed and reaction effect between the liquids in the reaction chamber 2-3 during detection. The vibration component 2-5 may not be in contact with the microfluidic chip 1. The vibration component 2-5 may be disposed at a position very close above the reaction chamber 1-3. Through the vibration of the vibration component 2-5, the vibration of the gas in the reaction chamber 1-3 is driven, thereby driving the vibration of the liquid in the reaction chamber 1-3 to achieve liquid mixing. This can avoid the problem of damage to the microfluidic chip 1 caused by vibration. Among them, the vibration component 2-5 may be a vibration motor, or other devices capable of generating vibration may be selected according to actual needs. The embodiments of this specification do not make specific limitations. Of course, the vibration component 2-3 may also be a rotatable component, such as a vibration rod. The vibration rod may be inserted into the reaction chamber 1-3. When it is necessary to mix the liquid, the liquid in the reaction chamber 1-3 can be mixed by the rotation or left-right and front-back swinging of the vibration rod. The principles and methods of the vibration component 2-5 for mixing the liquid in the reaction chamber 1-3 are not specifically limited in the embodiments of this specification.

[0068] As Figure 3 , Figure 4 shown, in some embodiments of this specification, there may be multiple reagent grooves 1-2 in the microfluidic chip 1. There may not only be a detection reagent groove and a color-developing reagent groove, but also a detergent reagent groove, which can be used to place a detergent reagent pack. The antibody liquid reagent pack in the detection reagent groove may include a labeled antibody and an immunomagnet. The labeled antibody can be used for an immunobinding reaction with the detection sample, and the immunomagnet can be used to adsorb specific proteins in the liquid after the immunobinding reaction. The specific protein can be understood as a specific protein, and the labeled antibody and immunomagnet in the antibody liquid reagent pack can be set according to the detection object, so that the immunomagnet can adsorb the designated protein. The detergent reagent pack is used to wash the immunomagnet after the immunobinding reaction, washing away other attachments on the immunomagnet except the adsorbed specific protein, so as to perform a color-developing reaction subsequently and improve the accuracy of the color-developing reaction.

[0069] As Figure 10As shown, in some embodiments of this specification, the detection device 2 may include an electromagnetic component 2-12. The electromagnetic component 2-12 is disposed below the position of the reaction chamber 1-3 of the microfluidic chip 1 and can be used to adsorb the immunomagnets in the antibody liquid reagent pack. Specifically, after the detection sample and the labeled antibody in the antibody liquid reagent pack undergo an immunobinding reaction in the reaction chamber 1-3, the immunomagnets can adsorb the specific proteins generated by the immunobinding reaction. However, the immunomagnets may avoid attaching other substances, which will affect the subsequent color reaction and thus affect the final detection result. Based on this, the embodiments of this specification also provide a washing liquid reagent pack. After the immunobinding reaction is completed, the rotation assembly 2-11 can be rotated to the corresponding position to control the puncture assembly 2-9 to puncture the washing liquid reagent pack in the detergent reagent tank, and release the reagent in the washing liquid reagent pack into the sample inlet 1-1. The liquid inlet driving component 2-6 and the electromagnetic component 2-12 are started, and the reagent in the washing liquid reagent pack is controlled to flow backward from the sample inlet 1-1. At the same time, the electromagnetic component 2-12 will adsorb the immunomagnets located in the reaction chamber 1-3, so that the immunomagnets can be fixed in the reaction chamber 1-3. The flowing washing liquid can wash the immunomagnets and wash away the substances attached to the surface of the immunomagnets. Then, the color developing liquid reagent pack is punctured, and the color developing liquid in the color developing liquid reagent pack is used to perform a color reaction with the washed immunomagnets to obtain the detection result.

[0070] Among them, immunomagnets can be understood as an immunological technique that combines the high specificity of immunological reactions with the unique magnetic responsiveness of magnets. Artificially synthesized magnets containing iron components that can be attracted by the magnetic force of a magnet and having functional groups on the outside that can bind active proteins (antibodies) can be used as a carrier for antibodies. When the antibodies on the magnet bind to the corresponding microorganisms or specific antigen substances, an antigen-antibody-magnet immunocomplex is formed. This complex has a high magnetic responsiveness and can separate the complex from other substances. Immunomagnets can be magnetic beads or other nanospheres with antibodies modified on their surfaces, or antibodies modified on the substrate, and can be specifically set according to actual needs. The embodiments of this specification do not make specific limitations.

[0071] As Figure 4 shown, in some embodiments of this specification, the microfluidic chip 1 may further include a buffer zone 1-4. The buffer zone 1-4 can be disposed between the reaction chamber 1-3 and the chip outlet 1-6. The buffer zone 1-4 can communicate with the reaction chamber 1-3 and the chip outlet 1-6. The buffer zone 1-4 can be set as a curved and / or relatively small-diameter groove, mainly used to prevent the liquid inlet driving component 2-6 from flushing the immunomagnets out of the reaction chamber 1-3 and into the chip outlet 1-6 when controlling the liquid flow in the microfluidic chip 1, such as when flushing the immunomagnets.

[0072] As Figure 4As shown, in some embodiments of this specification, the reagent tank 1-2 in the microfluidic chip 1 may further include a termination liquid reagent tank, which is used to place a termination liquid reagent pack. The reagent in the termination liquid reagent pack is mainly used to terminate the color reaction after it has occurred for a period of time, to avoid the instability of the liquid after the color reaction and prevent it from reacting with other substances, thus affecting the test results.

[0073] As Figure 4 shown, the reagent tanks 1-2 in the microfluidic chip 1 can be multiple, such as: 4, which are respectively a detection reagent tank, a detergent reagent tank, a color reagent tank, and a termination liquid reagent tank. Each reagent tank is connected to the sample inlet 1-1, facilitating the reagent in the reagent pack in the reagent tank to flow into the sample inlet 1-1 after the reagent pack is punctured. The multiple reagent tanks can be evenly arranged around the sample inlet, and the size and shape of the reagent tanks can be set according to actual needs, and are not specifically limited in the embodiments of this specification.

[0074] As Figure 6 shown, in some embodiments of this specification, the number of the puncture assemblies 2-9 is the same as the number of the reagent tanks 1-2 in the microfluidic chip 1. If there are two reagent tanks 1-2, then there are also two puncture assemblies 2-9. Figure 6 As shown, there are four reagent tanks 1-2, and the corresponding puncture assemblies 2-9 are also four. As Figure 6 described, in some embodiments of this specification, the puncture assembly 2-9 may include a puncture needle 2-9-4, and the puncture needles 2-9-4 are respectively located directly above the corresponding reagent tanks 1-2. As Figure 8 shown, a puncture needle opening 2-11-3 is provided in the rotation assembly 2-11. When the rotation assembly 2-11 rotates to a specified position, it can push the puncture needle 2-9-4 in the puncture assembly 2-9 to move downward through the puncture needle opening 2-11-3, thereby driving the puncture needle 2-9-4 to puncture the reagent pack in the reagent tank 1-2 below it. Each puncture needle 2-9-4 can respectively correspond to a different reagent tank 1-2. By rotating the rotation assembly 2-11 corresponding to the corresponding identifier, the control of the specified puncture needle 2-9-4 can be realized, and the corresponding reagent can be accurately released. Moreover, it can avoid the problem that the same puncture assembly 2-9 punctures different reagent packs, resulting in liquid contamination and affecting the test results.

[0075] In addition, a rotation motor 2-9-1 may be connected above the puncture assembly 2-9, and this rotation motor 2-9-1 may be in the rotation assembly 2-11. As Figure 9As shown, the rotary motor 2-9-1 can be used to drive the corresponding puncture assembly 2-9 to move up and down. The rotary assembly 2-9 may further include a support column 2-9-2 and a spring 2-9-3. The support column 2-9-2 is mainly used to support and fixedly connect the puncture needle 2-9-4 to the rotary motor 2-9-1. The spring 2-9-3 can be used for buffering to prevent the puncture assembly 2-9 from moving down too far and damaging the microfluidic chip 1.

[0076] In the embodiments of this specification, by providing a plurality of puncture assemblies 2-9, each puncture assembly 2-9 corresponding to a reagent tank 1-2, only by rotating the rotary assembly 2-11 so that the rotary assembly 2-11 corresponds to the corresponding indication mark, the corresponding puncture assembly 2-9 can be accurately controlled to pierce the corresponding reagent pack, realizing the accurate release of the reagent. The operation is simple and does not require professional skills, improving the efficiency and accuracy of the detection.

[0077] As Figure 6 shown, in some embodiments of this specification, the detection device 2 may further include a sterilization and disinfection assembly 2-10. The sterilization and disinfection assembly 2-10 can be arranged between the puncture assembly 2-9 and the reagent tank 1-2 of the microfluidic chip 1 for sterilizing and disinfecting the puncture assembly 2-9. After the puncture assembly 2-9 pierces the corresponding reagent pack, it may be contaminated with a small amount of reagent. After the detection is completed, the sterilization and disinfection assembly 2-10 can be started to sterilize and disinfect the puncture assembly 2-9, such as the puncture needle 2-9-4, to avoid affecting the detection result and improving the detection accuracy during subsequent use.

[0078] The sterilization and disinfection assembly 2-10 can select an ultraviolet lamp or other devices capable of sterilizing and disinfecting. Of course, according to actual needs, the sterilization and disinfection assembly 2-10 can also be not provided, and the puncture assembly 2-9 can be manually cleaned and disinfected.

[0079] As Figure 4 shown, in some embodiments of this specification, the microfluidic chip 1 further includes a detection cavity 1-5. The detection cavity 1-5 can be arranged at one end of the chip outlet 1-6 of the microfluidic chip 1. The detection cavity 1-5 is communicated with the reaction cavity 1-3. The detection cavity 1-5 is used to receive the reacted liquid discharged from the reaction cavity 1-3 after the detection is completed. The detection device can read the detection result based on the reacted liquid in the detection cavity 1-5.

[0080] Specifically, as Figure 5 shown, the detection device 2 may further include a detection result reading component 2-8, as Figure 10As shown in the figure, the detection result reading component 2-8 may include a photoelectric sensing receiving component 2-8-1 and a photoelectric sensing transmitting component 2-8-2. The photoelectric sensing transmitting component 2-8-2 is arranged below the detection chamber 1-5, and the photoelectric sensing receiving component 2-8-1 is arranged above the detection chamber 1-5. After the detection is completed, the detection chamber 1-5 contains the solution after the color reaction, and different solutions will correspond to different shades of color. After the detection is completed, the detection result reading component 2-8 can be turned on. The photoelectric sensing transmitting component 2-8-1 will emit a light beam towards the detection chamber 1-5. The light beam penetrates the detection chamber 1-5, part of it is absorbed, and part of it is received by the photoresistor of the photoelectric sensing transmitting component 2-8-2. The photoresistor will present different resistances according to the change of light intensity, thus showing different voltage values. In this way, the photoelectric sensing transmitting component 2-8-2 can show different voltage values according to the concentration of the reacted liquid in the detection chamber 1-5, and the corresponding detection result can be obtained based on this voltage value.

[0081] The mapping relationship between the detection results corresponding to different voltage values can be pre-drawn into a corresponding curve or table, and then the corresponding detection result can be quickly obtained based on the voltage value.

[0082] The embodiment of this specification utilizes the color reaction, that is, liquids with different concentrations can produce different shades of color, and then based on the absorbance of liquids with different shades of color, the detection result can be accurately and quickly obtained.

[0083] As Figure 5 shown, in some embodiments of this specification, the detection device further includes: a waste liquid collection component 2-7. The waste liquid collection component 2-7 is communicated with the liquid inlet driving component 2-6, so that the liquid inlet driving component 2-6 discharges the waste liquid generated by the detection into the waste liquid collection component 2-7 to realize the collection of waste liquid and avoid pollution caused by the waste liquid. For example: the liquid generated during the flushing process of the immunomagnet and the detected result. After the detection is completed, the liquid in the detection chamber can all be used as waste liquid and discharged into the waste liquid collection component 2-7.

[0084] As Figure 1 shown, a groove can be provided on the detection device housing 2-1. The waste liquid collection component handle 4 of the waste liquid collection component 2-7 can extend out of the detection device housing 2-1, and the waste liquid collection component 2-7 can be directly pulled out or placed in the corresponding position through the waste liquid collection component handle 4 to realize the rapid treatment of waste liquid.

[0085] In addition, as Figure 1 shown, the detection device 2 may further include a display screen 3, and the display screen 3 can be used to display the detection result and can also be used to display operation prompts.

[0086] In some embodiments of this specification, a control component may further be provided in the detection device 2. The control component may be communicatively connected to other components in the detection device, such as the vibration component 2-5, the liquid inlet driving component 2-6, the detection result reading component 2-8, the sterilization and disinfection component 2-10, the electromagnetic component 2-2, the display screen 3, etc. A working program may be set in the control component, and this working program may control the working processes and states of other components in the detection device 2. For example, when to start the liquid inlet driving component 2-6, how long the liquid inlet driving component 2-6 works before stopping after starting, then start the vibration component 2-5, how long the vibration component 2-5 works before stopping, and when to rotate the rotating component 2-11 to what position, and the position prompt can be displayed on the display screen 3 to prompt the user to perform relevant operations. Among them, the working duration of each component can be set according to the size of the microfluidic chip 1, the liquid flow rate, and the liquid volume, etc. For example, if the liquid in the sample inlet 1-1 is to be drawn into the reaction chamber 1-3, the liquid flow rate can be calculated according to the power situation of the liquid inlet driving component 2-6, and the working time of the liquid inlet driving component 2-6 can be designed according to the length from the sample inlet 1-1 to the reaction chamber 1-3 to draw the liquid in the sample inlet 1-1 into the reaction chamber 1-3. For example: the distance between the reaction chamber 1-3 and the sample inlet 1-1 is less than the distance between the detection chamber 1-5 and the sample inlet 1-1. If the liquid in the sample inlet 1-1 is to be drawn into the detection chamber 1-5, then the working time of the liquid inlet driving component 2-6 is longer than the working duration when the liquid in the sample inlet 1-1 is drawn into the reaction chamber 1-3.

[0087] Among them, the control component may be a device capable of data communication and control, such as a chip, a processor, etc. The specific form of the control component is not limited in the embodiments of this specification. The control component may be arranged below the display screen 3 of the detection device 2, or arranged at other positions with suitable space, which is not specifically limited in the embodiments of this specification.

[0088] Based on the control component, the automated operation of health detection can be realized. The user only needs to rotate the rotating component 2-11 to the corresponding position according to the prompt. After that, the liquid inlet driving component 2-6, the vibration component 2-5, the electromagnetic component 2-12, the detection result reading component 2-8, and the sterilization and disinfection component 2-10 can all be automatically turned on and off according to the working program, without manual intervention. The operation is simple and convenient. Health detection can be achieved without professional skills, improving the accuracy and convenience of detection, and avoiding the problem of detection failure caused by human misoperation.

[0089] Of course, corresponding operation manuals can also be provided, and the user can manually perform corresponding operations on the microfluidic detection system based on the operation steps provided in the manual.

[0090] In some embodiments of this specification, the microfluidic detection system may further include a detection data application program. The detection data application program can receive and display the detection results obtained in the above embodiments, and based on the detection results, it can analyze the health status of the corresponding detected object, and display the corresponding health status analysis and guidance information. The detection data application program can be installed on a user terminal such as a smart phone to realize the storage of health data, the real-time dynamic viewing of health data, the establishment of personal health records, the push of later management intervention plans, and the recommended diet and exercise plans, etc.

[0091] The microfluidic detection system provided in the embodiments of this specification can be used alone or in combination with other household daily necessities, such as in combination with products such as electric toothbrushes or dental irrigators. The embodiments of this specification do not limit specific usage scenarios.

[0092] For the microfluidic detection system provided in the embodiments of this specification, the detection time is short. It has been verified that it only takes about 10 minutes to obtain the result. Moreover, it is small and portable in structure, can be carried around at any time, has a low cost, and can realize self-examination for everyone at home. For different detection objects, only different detection reagent packs need to be replaced, and the data can also be directly transmitted to a personal mobile terminal to realize real-time dynamic monitoring.

[0093] Figure 11 It is a schematic flowchart of a microfluidic detection method in an embodiment of this specification. As Figure 11 shown, in some embodiments of this specification, a microfluidic detection method may also be provided. This method can be applied to the microfluidic detection system provided in the above embodiments. This method may include:

[0094] S110. Collect a detection sample and place the collected detection sample into the sample inlet of the microfluidic chip in the microfluidic detection system.

[0095] S120. Install the microfluidic chip into the chip installation slot of the detection device in the microfluidic detection system, rotate the rotating component in the detection device to the first indication position, and use the rotating component to control the puncturing component in the detection device to puncture the antibody liquid reagent pack in the detection reagent slot of the microfluidic chip, so that the reagent in the antibody liquid reagent pack flows into the sample inlet.

[0096] S130. Turn on the liquid inlet driving component in the detection device, and use the liquid inlet driving component to draw the detection sample in the sample inlet and the reagent in the antibody liquid reagent pack into the reaction chamber in the microfluidic chip, so that the reagent in the antibody liquid reagent pack and the detection sample undergo an immune binding reaction in the reaction chamber.

[0097] S140. After the first preset time, rotate the rotating component to the second indication position, and use the rotating component to control the puncturing component to puncture the chromogenic liquid reagent pack in the chromogenic reagent slot of the microfluidic chip.

[0098] S150. Activate the liquid inlet driving component in the detection device, and use the liquid inlet driving component to pump the reagent in the chromogenic liquid reagent pack into the reaction chamber, so that the reagent in the chromogenic liquid reagent pack reacts with the liquid after the immune binding reaction in the reaction chamber to produce a chromogenic reaction.

[0099] S160. After a second preset time, obtain the detection result based on the liquid after the reaction.

[0100] In a specific implementation process, when using the microfluidic system provided in the above embodiment for health detection, a microfluidic chip 1 can be taken out first. The microfluidic chip 1 can be placed in the chip installation groove 2-3 of the detection device 2 of the microfluidic system, or can be placed separately. It can be detected whether a reagent pack is placed in the reagent groove 1-2 of the microfluidic chip 1. If not, the reagent pack can be taken out and placed in the corresponding reagent groove 1-2. Then collect a detection sample such as saliva, blood or urine, etc., and place the collected detection sample into the sample inlet 1-1 of the microfluidic chip 1. Place the microfluidic chip 1 with the collected detection sample into the chip installation groove 2-3 of the detection device 2, and rotate the rotating component 2-11 in the detection device 2 to the first indication position, such as rotating the indication mark 2-11-1 of the rotating component 2-11 to the position indicating Figure 1 the 1 in it. After the rotating component 2-11 rotates to the corresponding position, the puncture component 2-9 can be controlled to move downward to pierce the antibody liquid reagent pack in the detection reagent groove, and the reagent in the antibody liquid reagent pack flows into the sample inlet 1-1.

[0101] Start the liquid inlet driving component 2-6, and use the liquid inlet driving component 2-6 to pump the liquid in the sample inlet 1-1 into the reaction chamber 1-3. The reagent in the antibody liquid reagent pack reacts with the detection sample in the reaction chamber 1-3 to produce an immune binding reaction. Among them, the working duration of the liquid inlet driving component 2-6 can be set according to the actual situation, and the working duration of the liquid inlet driving component 2-6 can be pre-written into the working program to realize the automatic operation of the liquid inlet driving component 2-6. The embodiments of this specification do not make specific limitations. In addition, referring to the description of the above embodiment, when the detection device 2 includes a vibration component 2-5, after the reagent in the antibody liquid reagent pack and the detection sample flow into the reaction chamber 1-3, the vibration component 2-5 can be activated to mix the reagent in the antibody liquid reagent pack and the detection sample to improve the reaction effect.

[0102] After the immunological binding reaction ends, for example, after a first preset time, the rotating component 2-11 can be rotated to a second indicated position, and the puncturing component 2-9 is controlled by the rotating component 2-11 to move downward to puncture the chromogenic liquid reagent pack in the chromogenic reagent tank, and the reagent in the chromogenic liquid reagent pack flows into the sample inlet 1-1. Then, the liquid inlet driving component 2-6 is started, and the liquid in the sample inlet 1-1 is pumped into the reaction chamber 1-3 by using the liquid inlet driving component 2-6, so that the reagent in the chromogenic liquid reagent pack reacts with the liquid after the immunological binding reaction in the reaction chamber 1-3 to produce a chromogenic reaction.

[0103] In addition, referring to the description of the above embodiments, the antibody liquid reagent pack may include a labeled antibody and an immunomagnet, the microfluidic chip 1 may further include a detergent reagent tank for placing a detergent reagent pack, and the detection device 2 further includes an electromagnetic component 2-12. After the immunological binding reaction ends and before the chromogenic reaction, the rotating component 2-11 can be rotated to a third indicated position, and the puncturing component 2-9 is controlled by the rotating component 2-11 to move downward to puncture the detergent reagent pack in the detergent reagent tank, and the reagent in the detergent reagent pack flows into the sample inlet 1-1. Then, the liquid inlet driving component 2-6 is started, and the liquid in the sample inlet 1-1 is pumped into the reaction chamber 1-3 by using the liquid inlet driving component 2-6. At the same time, the electromagnetic component 2-12 is started, so that the electromagnetic component 2-12 can adsorb the immunomagnet in the reaction chamber 1-3, thereby washing the immunomagnet with the reagent in the flowing detergent reagent pack to remove substances other than specific proteins in the immunomagnet and improving the accuracy of the detection result. After the washing is completed, the liquid after washing can be pumped into the waste liquid collection component 2-7 by using the liquid inlet driving component 2-6 for subsequent chromogenic reaction.

[0104] After the immunomagnet washing is completed, for example, after a second preset time, the operation of the chromogenic reaction is carried out, and the detection result is obtained based on the liquid after the chromogenic reaction. Among them, the specific values of the second preset time and the second preset time can be set according to the actual situation, and the embodiments of this specification do not make specific limitations.

[0105] Of course, referring to the description of the above embodiments, the microfluidic chip 1 may further include a termination liquid reagent tank for placing a termination liquid reagent pack, and the termination liquid reagent pack is used to terminate the chromogenic reaction.

[0106] The detection device 2 may further include other components such as a sterilization and disinfection component 2-10 for sterilizing and disinfecting the puncturing component 2-9 after the detection, a waste liquid collection component 2-7 for collecting the waste liquid generated during the detection process, and a detection result reading component 2-8 for detecting the absorbance of the liquid after the reaction to obtain the concentration of the liquid after the reaction, and further obtaining the detection result.

[0107] In addition, the detection device 2 may further include a control component. The control component can communicate with other components in the detection device 2. A working program can be pre-written in the control component, and this working program can control the working processes and states of other components in the detection device 2. For example, when to start the liquid inlet driving component 2-6, how long to work after the liquid inlet driving component 2-6 starts and then stop, then start the vibration component 2-5, how long to work after the vibration component 2-5 starts and then stop, and when to rotate the rotating component 2-11 to what position. The position prompt can be displayed on the display screen 3 to prompt the user to perform relevant operations. Based on the control component, automated operation of health detection can be realized. The user only needs to rotate the rotating component 2-11 to the corresponding position according to the prompt. After that, the liquid inlet driving component 2-6, the vibration component 2-5, the electromagnetic component 2-12, the detection result reading component 2-8, and the sterilization and disinfection component 2-10 can all be automatically turned on and off according to the working program without manual intervention. The operation is simple and convenient, and health detection can be achieved without professional skills.

[0108] Among them, based on the structure of the microfluidic system, there can be corresponding detection processes. The specific operations can refer to the description in the above embodiments and will not be elaborated here.

[0109] The following combines Figures 1 - 10 , and specifically introduces the structure of the microfluidic system provided in the embodiments of this specification and the method for performing microfluidic detection using this microfluidic system. Specifically, as Figures 1 - 10 shown, the microfluidic system provided in the embodiments of this specification mainly includes a detection device 2 and a microfluidic chip 1 with an integrated reagent pack. The microfluidic chip 1 can be realized by fabricating a mold through standard three-dimensional printing. As Figure 4 shown, the microfluidic chip 1 may include: a sample inlet 1-1, a reagent tank 1-2, a reaction chamber 1-3, a buffer zone 1-4, and a detection chamber 1-5. Among them, the reagent tank 1-2 can be used to place the reagent pack, and a groove can be provided between the sample inlet 1-1 and the reagent tank 1-2 to connect the sample inlet 1-1 and the reagent tank 1-2. Among them, the reagent pack can be heat-sealed with plastic and placed in a vacuum packaging bag for long-term storage. As Figure 4As shown in the figure, in some embodiments of this specification, there may be four reagent slots 1-2. The four reagent slots 1-2 can be respectively used to place different reagent packs. The reagent pack area can be respectively composed of four reagent water droplet packs. Among them, in some scenario examples, the reagents in each reagent pack can be: Reagent pack No. 1: Antibody solution reagent pack (transparent light yellow, HRP (horseradish peroxidase) secondary antibody-labeled protein and immunomagnetic beads); Reagent pack No. 2: Washing solution reagent pack (colorless transparent pack, 1×, TBST); Reagent pack No. 3: Chromogenic solution reagent pack (black opaque pack, TMB (Tetramethylbenzidine, a common substrate for horseradish peroxidase)); Reagent pack No. 4: Termination solution reagent pack (colorless transparent pack, sulfuric acid). The rest of the microfluidic chip 1 can be formed by casting with PDMS as the main raw material. The upper layer and the lower layer can be bonded with a clean glass by plasma for fixation and support.

[0110] As Figure 1 shown, the shell of the detection device 2 provided in the examples of this specification can be three-dimensionally printed with ABS (a thermoplastic polymer structural material, mainly synthesized by chemical substances such as acrylonitrile and butadiene). The overall shape is a square black box. The upper surface of the detection device 2 is a display screen 3 and two buttons (power-on button, detection button); the left side is the card slot opening of the microfluidic chip 1 (a four-row reagent pack can be inserted), and the rear side is the extraction port of the waste liquid collection component 2-7. Among them, the detection device 2 can include the following components:

[0111] Sampling module 2-4: It is composed of two parts, a puncture component 2-9 and a rotation component 2-11. Among them, the puncture component 2-9 can include a puncture needle 2-9-4, a spring 2-9-3, etc. The sampling principle is: The reagent slot 1-2 of the microfluidic chip 1 is aligned with the bottom of the puncture needle 2-9-4. Through the action of gravity and the rotation drive mode of the rotation component 2-11, the liquid in the reagent pack flows into the sampling port 1-1 in sequence, and then the liquid is accurately quantitatively controlled to enter the reaction chamber 1-3 through the liquid inlet drive component 2-6.

[0112] Vibration component 2-5: The vibration component 2-5 can be a vibration motor, which is set directly above the reaction chamber 1-3 of the microfluidic chip 1. When it is turned on, the working voltage can be 4v.

[0113] Electromagnetic component 2-12: The electromagnetic component 2-12 can select an electromagnet, which is set directly below the reaction chamber 1-3. When it is turned on, the working voltage can be 24v.

[0114] Liquid inlet driving component 2-6: The liquid inlet driving component 2-6 can select a pump as the driving device. The liquid inlet driving component 2-6 can be connected to the chip outlet 1-6 of the microfluidic chip 1 to drive the flow of the liquid in the microfluidic chip 1 and play a role in discharging waste liquid.

[0115] Detection result reading component 2-8: The detection result reading component 2-8 can adopt a photoelectric sensor, such as a photoelectric sensor with an LED lamp. For example, there can be a photoelectric sensing receiving component 2-8-1 and a photoelectric sensing transmitting component 2-8-2 symmetrically arranged at positions directly above and below the detection cavity 1-5 of the microfluidic chip 1. The working voltage of the photoelectric sensor can be 5V.

[0116] Sterilization and disinfection component 2-10: An ultraviolet lamp can be selected as the sterilization and disinfection component 2-10. It is arranged between the microfluidic chip 1 and the puncture needle 2-9-4 and aligned with the puncture needle 2-9-4, and can be used to sterilize the puncture needle 2-9-4.

[0117] Display screen 3: It can be set directly above the whole detection device 2 and is used to display the user's real-time health detection data.

[0118] Waste liquid collection component 2-7: It is used for waste liquid collection, and the user can pour the waste liquid after each detection.

[0119] In a scenario example of this specification, the process of using the above microfluidic detection system to detect saliva samples can be referred to as follows:

[0120] 1. Press the "Power key"; (Put the instrument in the standby state, and the display screen 3 shows "Initial state").

[0121] 2. Take out the microfluidic chip 1, collect saliva into the sample inlet 1-1 of the microfluidic chip 1 outside the body, and insert it into the chip installation slot 2-3 of the detection device.

[0122] 3. Turn on the "Detection key", and the display screen 3 shows "Please rotate to gear 1".

[0123] 4. Manually rotate the sample injection rotating component 2-11 from the initial position to the indicating position corresponding to gear 1, which can push the puncture component 2-9 downward to pierce the No. 1 reagent pack. After the No. 1 reagent pack is punctured, the reagent in it flows into the sample inlet 1-1 to get ready.

[0124] 5. Turn on the liquid inlet driving component 2-6, and it will automatically pause after working for 5 seconds. The flow rate is about 300 ul / min. The sample in the sample inlet 1-1, the labeled antibody and immunomagnet in the No. 1 reagent pack are drawn into the reaction cavity 1-3.

[0125] 6. Pause for 3 seconds until the liquid stabilizes and flows over the immunomagnetic beads in the reaction chamber 1-3 after flowing through the reagent pack No. 1.

[0126] 7. Turn on the vibration component 2-5, which will automatically turn off after working for 3 minutes, for immunological binding reaction.

[0127] 8. Turn on the liquid inlet driving component 2-6, which will automatically pause after working for 20 seconds, and pump all the waste liquid in the microfluidic chip 1 into the waste liquid collection component 2-7. At this time, the display screen 2 shows "Please switch to the second gear".

[0128] 9. Manually rotate the sampling rotary component 2-11 to the second gear at the corresponding indicated position, then the puncture component 2-9 can be pushed downward to pierce the reagent pack No. 2. After the reagent pack No. 2 is punctured, the reagent therein flows into the sample inlet 1-1, getting ready.

[0129] 10. Turn on the electromagnetic component 2-12 and the liquid inlet driving component 2-6 simultaneously, which will automatically pause after working for 60 seconds, with a flow rate of about 100 μl / min. Quickly flush the washing solution in the reagent pack No. 2 in the sample inlet 1-1 through the reaction chamber 1-3 to complete the operation of washing the immunomagnetic beads. The waste liquid flows into the waste liquid collection component 2-7. At this time, the display screen 3 shows "Please switch to the third gear".

[0130] 11. Manually rotate the sampling rotary component 2-11 to the third gear at the corresponding indicated position, then the puncture component 2-9 can be pushed downward to pierce the reagent pack No. 3. After the reagent pack No. 3 is punctured, the reagent therein flows into the sample inlet 1-1, getting ready.

[0131] 12. Turn on the liquid inlet driving component 2-6, which will automatically pause after working for 5 seconds, with a flow rate of about 300 μl / min. Pump the chromogenic solution in the reagent pack No. 3 in the sample inlet 1-1 into the reaction chamber 1-3.

[0132] 13. Pause for 3 seconds until the liquid stabilizes and flows over the immunomagnetic beads in the reaction chamber 1-3.

[0133] 14. Turn off the electromagnetic component 2-12, turn on the vibration component 2-5, which will automatically turn off after working for 5 minutes; at this time, the display screen shows "Please switch to the fourth gear".

[0134] 15. Manually rotate the sampling rotary component 2-11 to the fourth gear at the corresponding indicated position, then the puncture component 2-9 can be pushed downward to pierce the reagent pack No. 4. After the reagent pack No. 3 is punctured, the reagent therein flows into the sample inlet 1-1, getting ready.

[0135] 16. Turn on the liquid inlet driving component 2-6, which will automatically turn off after working for 20 seconds, with a flow rate of about 300 μl / min. Pump the termination solution in the reagent pack No. 4 in the sample inlet 1-1 into the reaction chamber 1-3 and then directly flow it into the detection chamber 1-5.

[0136] 17. The detection result reading component 2-8 automatically opens for detection reading; after the detection result is directly presented on the display screen 3, the detection result reading component 2-8 automatically closes.

[0137] 18. When the detection process ends, the sterilization and disinfection component 2-10 is automatically turned on for automatic sterilization and disinfection, facilitating subsequent repeated use.

[0138] 19. After the detection is completed, the microfluidic chip 1 and the waste liquid collection component 2-7 are removed.

[0139] Among them, the opening time, working duration, closing time, etc. of the above-mentioned liquid inlet driving component 2-6, vibration component 2-5, electromagnetic component 2-12, detection result reading component 2-8, sterilization and disinfection component 2-10, etc. can be pre-written into the working program of the control component of the detection device 2, and the control component is used to automatically control the working states of other components to achieve semi-automatic detection.

[0140] The microfluidic detection system provided by the embodiments of this specification has a simple structure, small volume, is easy to carry, and has a short detection time, is easy to operate, and can achieve health detection without professional technology, and can be suitable for most users. In addition, different detection objects can be detected by replacing the reagent pack in the microfluidic chip according to the detection needs.

[0141] Obviously, the above-described embodiments are only a part of the embodiments of this specification, rather than all embodiments. Based on the embodiments in this specification, those of ordinary skill in the art can make other different forms of changes or modifications without creative efforts, and all should belong to the scope protected by this specification.

[0142] After considering the specification and practicing the invention disclosed herein, those skilled in the art will readily think of other implementation schemes of the embodiments of this specification. This specification is intended to cover any variations, uses, or adaptive changes of the embodiments of this specification, and these variations, uses, or adaptive changes follow the general principles of the embodiments of this specification and include the common general knowledge or conventional technical means in the technical field not disclosed in this specification. The specification and embodiments are only regarded as exemplary, and the true scope and spirit of the embodiments of this specification are pointed out by the following claims.

[0143] It should be understood that the embodiments of this specification are not limited to the exact structures already described and shown in the drawings, and various modifications and changes can be made without departing from its scope. The scope of the embodiments of this specification is only limited by the appended claims.

Claims

1. A microfluidic detection system, characterized in that, The microfluidic detection system includes: a microfluidic chip and a detection device. The detection device is provided with a chip installation slot, a sampling module, and a liquid inlet driving component; the chip installation slot is used to place the microfluidic chip; the microfluidic chip includes: a sampling port, a reagent tank, and a reaction chamber; The reagent tank includes a detection reagent tank and a color-developing reagent tank. The detection reagent tank is used to place an antibody solution reagent pack, and the color-developing reagent tank is used to place a color-developing solution reagent pack; the reagent in the antibody solution reagent pack is used to perform an immune binding reaction with the detection sample, and the color-developing solution reagent pack is used to perform a color-developing reaction with the liquid after the immune binding reaction; The sampling port is communicated with both the reagent tank and the reaction chamber, and is used for the detection sample and the reagent in the reagent tank to flow into the reaction chamber to react after sampling; The sampling module includes a puncture component and a rotation component. The bottom of the puncture component is located above the reagent tank, and the rotation component is used to control the puncture component to move downward so that the puncture component pierces the reagent pack in the reagent tank; The liquid inlet driving component is communicated with the chip outlet of the microfluidic chip and is used to control the liquid flow in the microfluidic chip.

2. The microfluidic detection system according to claim 1, wherein The number of the puncture components is the same as the number of the reagent tanks, and each puncture component corresponds to a corresponding reagent tank. The rotation component is used to respectively control each puncture component to move downward by rotation to pierce the reagent pack in the corresponding reagent tank.

3. The microfluidic detection system according to claim 1, wherein The detection device further includes a sterilization and disinfection component, which is arranged between the puncture component and the reagent tank of the microfluidic chip and is used to sterilize and disinfect the puncture component.

4. The microfluidic detection system according to claim 1, characterized in that, The detection device further includes a vibration component, which is arranged above the reaction chamber and is used to mix the liquid in the reaction chamber.

5. The microfluidic detection system according to claim 1, wherein The reagent tank further includes a detergent reagent tank, which is used to place a washing liquid reagent pack. The antibody solution reagent pack includes a labeled antibody and an immunomagnet. The labeled antibody is used to perform an immune binding reaction with the detection sample, and the immunomagnet is used to adsorb the specific protein in the liquid after the immune binding reaction; The detection device further includes an electromagnetic component, which is arranged below the reaction chamber of the microfluidic chip and is used to adsorb the immunomagnet. After the labeled antibody performs an immune binding reaction with the detection sample, the puncture component pierces the washing liquid reagent pack, and the liquid inlet driving component pumps the reagent in the washing liquid reagent pack into the reaction chamber to wash the immunomagnet adsorbed by the electromagnetic component.

6. The microfluidic detection system according to claim 5, characterized in that, The microfluidic chip further includes a buffer area, which is arranged between the reaction chamber and the chip outlet. The buffer area is communicated with both the reaction chamber and the chip outlet, and the buffer area is used to prevent the immunomagnet from being washed out of the reaction chamber when the liquid in the microfluidic chip flows.

7. The microfluidic detection system according to claim 1, wherein The reagent tank further includes a termination liquid reagent tank, which is used to place a termination liquid reagent pack, and the termination liquid reagent pack is used to terminate the color-developing reaction.

8. The microfluidic detection system according to claim 1, wherein The microfluidic chip further includes a detection chamber, which is arranged at the chip outlet end of the microfluidic chip. The detection chamber is communicated with the reaction chamber and is used for receiving the post-reaction liquid discharged from the reaction chamber after the detection is completed. The detection device further includes a detection result reading component. The detection result reading component includes a photoelectric sensing emission component and a photoelectric sensing receiving component. The photoelectric sensing emission component is arranged below the detection chamber, and the photoelectric sensing receiving component is arranged above the detection chamber. The photoelectric sensing emission component is used for emitting a light beam to the detection chamber after the detection is completed, and the photoelectric sensing receiving component is used for receiving the light beam passing through the post-reaction liquid in the detection chamber and displaying different voltage values based on the received light beam to obtain the detection result.

9. The microfluidic detection system according to claim 1, wherein The detection device further includes a waste liquid collection component, which is communicated with the liquid inlet driving component so that the liquid inlet driving component discharges the waste liquid generated by the detection into the waste liquid collection component.

10. The microfluidic detection system according to any one of claims 1-9, characterized in that The detection device further includes a control component, which is communicatively connected to each component in the detection device. A working program is set in the control component, and the working program is used for controlling the working processes and working states of each component in the detection device.

11. The microfluidic detection system according to claim 1, wherein The microfluidic detection system further includes a detection data application program, which is used for receiving and displaying the detection result and displaying the corresponding health status analysis and guiding information based on the detection result.

12. A microfluidic detection method, characterized in that, The detection method is applied to the microfluidic detection system according to any one of claims 1-11, and the detection method includes: Collecting a detection sample and placing the collected detection sample into the sample inlet of the microfluidic chip in the microfluidic detection system. Installing the microfluidic chip into the chip installation slot of the detection device in the microfluidic detection system, rotating the rotating component in the detection device to the first indication position, and using the rotating component to control the puncturing component in the detection device to puncture the antibody liquid reagent package in the detection reagent tank of the microfluidic chip so that the reagent in the antibody liquid reagent package flows into the sample inlet. Turning on the liquid inlet driving component in the detection device and using the liquid inlet driving component to suck the detection sample in the sample inlet and the reagent in the antibody liquid reagent package into the reaction chamber in the microfluidic chip, so that the reagent in the antibody liquid reagent package undergoes an immunological binding reaction with the detection sample in the reaction chamber. After a first preset time, rotating the rotating component to the second indication position and using the rotating component to control the puncturing component to puncture the chromogenic liquid reagent package in the chromogenic reagent tank of the microfluidic chip. Turning on the liquid inlet driving component in the detection device and using the liquid inlet driving component to suck the reagent in the chromogenic liquid reagent package into the reaction chamber so that the reagent in the chromogenic liquid reagent package undergoes a chromogenic reaction with the liquid after the immunological binding reaction in the reaction chamber. After a second preset time, obtaining the detection result based on the post-reaction liquid.

Images