Cleaning and separating device and using method thereof

Abstract

The invention discloses a cleaning and separating device and a using method thereof, and belongs to the field of chemiluminescence immunoassay. A mounting frame comprises a vertical plate and a transverse plate fixed to the vertical plate, and a cleaning needle assembly and a gripper assembly are in transmission connection with a translation driving part; the cleaning station assembly, the magnetic separation assembly and the uniform mixing assembly are fixed to the transverse plate and located on the same straight line, the mounting column comprises an end, the diameter of an adjusting hole of the transverse plate is larger than that of the end, and the end is located in the adjusting hole and can adjust the position so that the position of the mounting frame can be adjusted relative to the mounting column. The cleaning station assembly, the magnetic separation assembly and the uniform mixing assembly can be integrally mounted and dismounted and are located on the same straight line, so that the gripper assembly is convenient to move.

Description

Technical Field

[0001] The invention relates to the field of chemiluminescent immunoassay, and in particular to a cleaning and separation device and a use method thereof. Background Art

[0002] Chemiluminescent immunoassay combines a chemical reaction system with the immune system to detect antigens or antibodies. Labeling antigens and antibodies with chemiluminescent substances has both the specificity of immune reactions and the high sensitivity of chemiluminescent reactions. The operation method is simple and convenient, with good repeatability and no pollution, and is widely used in clinical practice.

[0003] Since the fully automatic chemiluminescence immunoassay instrument uses a fully automatic control method to realize the immunobiochemical reaction process outside the human body and detects and quantitatively analyzes the results of the reaction process, the reaction cup needs to be cleaned, separated, mixed, and other operations during the reaction process, so corresponding parts are required. The structural design of the existing cleaning and separation device is unreasonable, resulting in complex installation and difficult disassembly of parts; and the movement path of the translational parts is unreasonable, resulting in a long cleaning and separation process, low efficiency, and difficulty in improving throughput. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, one of the objects of the present invention is to provide a cleaning and separation device with simple structure, easy installation and high efficiency.

[0005] In order to overcome the deficiencies of the prior art, a second object of the present invention is to provide a method for using a cleaning and separation device with a simple structure, easy installation and high efficiency.

[0006] One of the purposes of the present invention is achieved by the following technical solution:

[0007] The cleaning and separation device comprises a mounting frame, wherein the mounting frame comprises a vertical plate and a horizontal plate fixed to the vertical plate, the cleaning and separation device also comprises a mounting column, a positioning plate, a cleaning needle assembly, a gripper assembly, a cleaning station assembly, a magnetic separation assembly, a mixing assembly and a translation drive assembly, the translation drive assembly comprises a translation drive member, a rotating shaft and a belt, the cleaning needle assembly and the gripper assembly are transmission-connected with the translation drive member, the cleaning station assembly, the magnetic separation assembly and the mixing assembly are fixed to the horizontal plate and are located on the same straight line, the magnetic separation assembly is located between the cleaning station assembly and the mixing assembly, the mounting column comprises an end portion, the horizontal plate is provided with an adjustment hole, the diameter of the adjustment hole is larger than the diameter of the end portion, the end portion is located in the adjustment hole and can adjust its position so that the position of the mounting frame relative to the mounting column can be adjusted, and the positioning plate fixes the mounting column and the mounting frame.

[0008] Further, the cross plate is also provided with fixing holes, the positioning piece is provided with locking holes and a positioning groove, the locking holes are fixed to the end of the mounting column, the position of the positioning groove corresponds to that of the fixing holes, and a screw passes through the fixing holes and extends into the positioning groove and cooperates with a nut to fix the mounting column and the mounting bracket.

[0009] Further, the translation driving member is fixed to the vertical plate, the number of the rotating shafts is multiple, the multiple rotating shafts are rotatably mounted on the vertical plate, the belt is sleeved on the rotating shafts, the output end of the translation driving member is fixedly connected to one of the rotating shafts, and the cleaning needle assembly and the gripper assembly are connected to the belt.

[0010] Further, the cleaning needle assembly includes a base, a bearing and a cleaning needle, the base is mounted on the vertical plate, the bearing is mounted in the base, and the cleaning needle is rotatably mounted on the base through the bearing.

[0011] Further, the cleaning needle assembly further includes a vertical driving member and a rack, a gear is mounted on the output end of the vertical driving member, and the vertical gear meshes with the rack to drive the base to move in the vertical direction.

[0012] Further, the magnetic separation assembly includes a separation base and a magnet, the separation base is fixed to the cross plate, and the magnet is mounted inside the separation base.

[0013] Further, the number of the magnets is four, the four magnets are arranged in a cross shape, and the two magnets located on the same straight line have the same magnetic property, and a space for accommodating the reaction cup is formed between the four magnets.

[0014] Further, the mixing component includes a mixing motor, a mixing base and a mixing head, the mixing motor is fixed to the cross plate, the mixing base is mounted on the output end of the mixing motor, the mixing motor drives the mixing base to rotate, the mixing head is mounted on the mixing base, and the mixing head is used for mounting the reaction cup.

[0015] Further, an included angle is formed between the axis of the mixing head and the axis of the mixing base.

[0016] The second object of the present invention is achieved by adopting the following technical solution:

[0017] The using method of any of the above cleaning and separating devices includes the following steps:

[0018] Install the mounting bracket on the mounting column, and after adjusting the position of the mounting bracket, lock the mounting bracket and the mounting column through the positioning piece;

[0019] The translation drive assembly drives the gripper assembly to translate to the incubation tray, grabs the reaction cups on the incubation tray and moves the reaction cups to the magnetic separation assembly;

[0020] The magnet attracts the magnet in the reaction cup, the liquid suction needle of the cleaning needle assembly sucks the liquid in the reaction cup, and the dispensing needle of the cleaning needle assembly adds buffer solution to the reaction cup to realize the cleaning of the reactants in the reaction cup;

[0021] The gripper assembly grabs the reaction cup on the magnetic separation assembly to the mixing assembly, the mixing motor drives the mixing base to rotate, and the reactants in the reaction cup are mixed;

[0022] The gripper assembly grabs the reaction cup on the mixing assembly to the incubation tray, and the incubation tray incubates the reactants in the reaction cup;

[0023] The cleaning needle assembly completes one cycle of actions and moves to the cleaning station for cleaning.

[0024] Compared with the prior art, the cleaning and separation device of the present invention has the following advantages:

[0025] (1) The mounting post includes an end portion, the transverse plate is provided with an adjustment hole, the diameter of the adjustment hole is larger than the diameter of the end portion, and the end portion is located in the adjustment hole and can adjust its position so that the position of the mounting frame relative to the mounting post can be adjusted, and the positioning piece fixes the mounting post and the mounting frame.

[0026] (2) The cleaning station assembly, the magnetic separation assembly and the mixing assembly are fixed to the transverse plate and are located on the same straight line, so that the cleaning station assembly, the magnetic separation assembly and the mixing assembly can be integrally installed and disassembled. The cleaning station assembly, the magnetic separation assembly and the mixing assembly are located on a straight line, which is convenient for the gripper assembly to move;

[0027] (3) The cleaning needle is rotatably mounted on the base through a bearing, and the cleaning needle is connected to the pipeline. When the pipeline makes a loop, the cleaning needle rotates, which can avoid the pipeline from making a loop.

[0028] (4) The four magnets are arranged in a cross shape, and the two magnets located on the same straight line have the same magnetic property, so that the magnets can better adsorb the magnetic beads in the reaction cup;

[0029] (5) The axis of the mixing head forms an angle with the axis of the mixing base, so that when the mixing base rotates, the reactants in the reaction cup in the mixing head are mixed more evenly;

[0030] (6) Since the cleaning needle assembly and the gripper assembly take less time to complete a sample cycle, while the magnetic separation assembly and the mixing assembly take longer, increasing the number of magnetic separation assemblies and mixing assemblies allows for the use of one set of gripper assemblies and one set of needle assemblies in conjunction with two or more sets of magnetic separation assemblies and mixing assemblies. By integrating the time cycles occupied by different components within the cleaning and separation device, the average required time can be reduced, the usage efficiency of the needles and grippers can be improved, and the test throughput of the entire device can be increased. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a perspective view of the cleaning and separation device of the present invention;

[0032] Figure 2 is Figure 1 a perspective view of the mounting rack of the cleaning and separation device of

[0033] Figure 3 is Figure 2 another perspective view of the mounting rack of

[0034] Figure 4 is Figure 1 a perspective view of a partial structure of the cleaning and separation device of

[0035] Figure 5 is Figure 1 the front view of the cleaning and separation device of

[0036] Figure 6 is Figure 1 a schematic structural diagram of the magnetic separation assembly of the cleaning and separation device of

[0037] Figure 7 is a flowchart of the odd cycle of the usage method of the cleaning and separation device of the present invention;

[0038] Figure 8 is a flowchart of the even cycle of the usage method of the cleaning and separation device of the present invention.

[0039] In the figure: 21, mounting post; 210, end; 22, mounting bracket; 220, vertical plate; 221, horizontal plate; 2210, adjustment hole; 2211, fixing hole; 23, positioning piece; 230, positioning groove; 231, locking hole; 24, cleaning needle assembly; 240, vertical driving member; 241, rack; 242, base; 243, bearing; 244, cleaning needle; 25, gripper assembly; 26, cleaning station assembly; 27, magnetic separation assembly; 270, heating member; 271, separation base; 272, magnet; 273, first magnetic separation assembly; 274, second magnetic separation assembly; 28, mixing assembly; 280, mixing motor; 281, mixing base; 282, mixing head; 283, first mixing assembly; 284, second mixing assembly; 29, translation driving assembly; 290, translation driving member; 291, rotating shaft; 292, belt; 100, reaction cup. Detailed implementation manners

[0040] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0041] It should be noted that when a component is referred to as being "fixed to" another component, it can be directly on the other component or there may also be another intermediate component through which it is fixed. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for the purpose of illustration.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this invention belongs. The terms used herein in the description of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0043] Please refer to Figure 1, the cleaning and separation device includes a mounting post 21, a mounting frame 22, a positioning piece 23, a cleaning needle assembly 24, a gripper assembly 25, a cleaning station assembly 26, a magnetic separation assembly 27, a mixing assembly 28, and a translation drive assembly 29. The mounting frame 22 is mounted on the mounting post 21 through the positioning piece 23, and its position can be adjusted. The cleaning needle assembly 24 and the gripper assembly 25 are slidably mounted on the mounting frame 22, facilitating the grasping of the reaction cup 100 and the cleaning of the reactants inside the reaction cup 100. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are fixed to the mounting frame 22 and are located on the same straight line. This design enables the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 to be installed or disassembled together, facilitating the assembly of the device. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are located on the same straight line, enabling the cleaning needle assembly 24 and the gripper assembly 25 to operate on any one of the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 when moving linearly along the mounting frame 22. The translation drive assembly 29 is mounted on the mounting frame 22 for driving the cleaning needle assembly 24 and the gripper assembly 25 to translate.

[0044] Please continue to refer to Figure 2 and Figure 3 , the mounting post 21 includes an end 210, and the end 210 is circular.

[0045] The mounting frame 22 includes a vertical plate 220 and a horizontal plate 221, and the vertical plate 220 is fixedly connected to the horizontal plate 221. The vertical plate 220 is used to mount the translation drive assembly 29, and the cleaning needle assembly 24 and the gripper assembly 25 are slidably mounted on the vertical plate 220. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are fixed to the horizontal plate 221. The horizontal plate 221 is provided with an adjustment hole 2210 and a fixing hole 2211. The adjustment hole 2210 is circular, and the diameter of the adjustment hole 2210 is larger than the diameter of the end 210, enabling the position of the mounting frame 22 to be adjusted when it is mounted on the mounting post 21.

[0046] The positioning piece 23 is provided with a locking hole 231 and a positioning groove 230. The locking hole 231 cooperates with the end 210, and the position of the positioning groove 230 corresponds to the fixing hole 2211. A locking member is installed in the fixing hole 2211 and the positioning groove 230 to lock the positioning piece 23 to the horizontal plate 221. Specifically, the positioning groove 230 is U-shaped.

[0047] Please continue to refer to Figure 4, the cleaning needle assembly 24 includes a vertical driving member 240, a rack 241, a base 242, a bearing 243, and a cleaning needle 244. The rack 241 is slidably mounted on the vertical plate 220, the vertical driving member 240 is slidably mounted on the rack 241, a gear is provided on the output shaft of the vertical driving member 240, and the gear meshes with the rack 241. The base 242 is connected to the vertical driving member 240 and moves vertically with the vertical driving member 240. The bearing 243 is installed in the base 242, and the cleaning needle 244 is rotatably mounted in the base 241 through the bearing 243. The cleaning needle 244 includes a liquid suction needle and a cleaning needle, and the liquid suction needle is parallel to the cleaning needle. Both the liquid suction needle and the cleaning needle are connected to a pipeline. Since the cleaning needle 244 is rotatably mounted in the base 241, when the pipeline makes a loop, the cleaning needle 244 rotates in the base 241, which can avoid the pipeline from knotting.

[0048] The gripper assembly 25 is slidably mounted on the vertical plate 220, and the gripper assembly 25 is used to grasp and release the reaction cup 100.

[0049] The cleaning station assembly 26 is fixed to the horizontal plate 221.

[0050] The magnetic separation assembly 27 includes a heating member 270, a separation base 271, and a magnet 272. The separation base 271 is fixed to the horizontal plate 221, and the heating member 270 is installed at the bottom of the separation base 271 to control the temperature of the separation base 271. Specifically, the heating member 270 is a Peltier. The magnet 272 is installed inside the separation base 271, and the magnet 272 attracts the magnetic beads in the reaction cup 100 to facilitate the cleaning of the reactants. Specifically, please continue to refer to Figure 6 , the number of the magnets 272 is four, the four magnets 272 are arranged in a cross shape, and the two magnets 272 on the same straight line have the same magnetism. A space for accommodating the reaction cup is formed between the four magnets 272. In this embodiment, the number of the magnetic separation assemblies 27 is two, and the two magnetic separation assemblies 27 are arranged in parallel.

[0051] The mixing assembly 28 includes a mixing motor 280, a mixing base 281, and a mixing head 282. The mixing motor 280 is fixed to the horizontal plate 221, the mixing base 281 is installed at the output end of the mixing motor 280, and the mixing motor 280 drives the mixing base 281 to rotate around an axis. The mixing head 282 is installed on the mixing base 281, and the axis of the mixing head 282 forms an angle with the axis of the mixing base 281, so that the reactants in the reaction cup 100 installed on the mixing head 282 are mixed more evenly. In this embodiment, the number of the mixing assemblies 28 is two, and the two mixing assemblies 28 are arranged in parallel.

[0052] Please continue to refer to Figure 1, the translation drive assembly 29 includes a translation drive member 290, a rotating shaft 291, and a belt 292. The translation drive member 290 is fixed to the vertical plate 220. The number of rotating shafts 291 is two, and the two rotating shafts 291 are rotatably mounted on the vertical plate 220. The belt 292 is sleeved between the two rotating shafts 291. The translation drive member 290 is in transmission connection with one rotating shaft 291. The translation drive member 290 drives the rotating wheel to rotate, causing the belt 292 to rotate. The cleaning needle assembly 24 and the gripper assembly 25 are mounted on the belt 292 and are driven by the belt 292 to translate and move between the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28. The number of translation drive assemblies 29 is two, and the cleaning needle assembly 24 and the gripper assembly 25 are respectively mounted on the two translation drive assemblies 29, so that the cleaning needle assembly 24 and the gripper assembly 25 are respectively driven without interference.

[0053] When using the cleaning and separation device, the mounting frame 22 is mounted on the mounting column 21. After adjusting the position of the mounting frame 22, the mounting frame 22 is locked with the mounting column 21 through the positioning piece 23; the translation drive assembly 29 drives the gripper assembly 25 to translate to the incubation tray, grabs the reaction cup 100 on the incubation tray and moves the reaction cup 100 to the magnetic separation assembly 27; the magnet 272 in the reaction cup 100 is attracted by the magnet 272, the liquid suction needle of the cleaning needle 244 assembly 24 sucks the liquid in the reaction cup 100, and the dispensing needle of the cleaning needle 244 assembly 24 adds the buffer solution to the reaction cup 100 to realize the cleaning of the reactants in the reaction cup 100; the gripper assembly 25 grabs the reaction cup 100 on the magnetic separation assembly 27 to the mixing assembly 28, and the mixing motor 280 drives the mixing base 281 to rotate, and the reactants in the reaction cup 100 are mixed; the gripper assembly 25 grabs the reaction cup 100 on the mixing assembly 28 to the incubation tray, and the incubation tray incubates the reactants in the reaction cup 100. When using the cleaning and separation device, it involves the rotation of the incubation tray, and the incubation tray rotates to send the reaction cup 300. The incubation tray judges whether to send the cup according to the control signal. When it is necessary to send the cup, it judges whether there is a reaction cup 100 at the cup sending position of the incubation tray. When there is a reaction cup 100, the gripper assembly 25 grabs the cup.

[0054] The present application also discloses a usage method of the above-mentioned cleaning and separation device, including the following steps:

[0055] Mount the mounting frame 22 on the mounting column 21. After adjusting the position of the mounting frame 22, lock the mounting frame 22 with the mounting column 21 through the positioning piece 23;

[0056] The translation drive assembly 29 drives the gripper assembly 25 to translate to the incubation tray, grabs the reaction cup 100 on the incubation tray and moves the reaction cup 100 to the magnetic separation assembly 27;

[0057] The magnet 272 attracts the magnet 272 in the reaction cup 100. The liquid suction needle of the cleaning needle 244 assembly 24 sucks the liquid in the reaction cup 100, and the dispensing needle of the cleaning needle 244 assembly 24 adds buffer solution to the reaction cup 100 to realize the cleaning of the reactants in the reaction cup 100.

[0058] The gripper assembly 25 grabs the reaction cup 100 on the magnetic separation assembly 27 to the mixing assembly 28. The mixing motor 280 drives the mixing base 281 to rotate, and the reactants in the reaction cup 100 are mixed.

[0059] The gripper assembly 25 grabs the reaction cup 100 on the mixing assembly 28 to the incubation tray, and the incubation tray incubates the reactants in the reaction cup 100.

[0060] During specific use, the incubation tray rotates, and the two magnetic separation assemblies 27 and the two mixing assemblies 28 work alternately. The two magnetic separation assemblies 27 are the first magnetic separation assembly 273 and the second magnetic separation assembly 274 respectively, and the two mixing assemblies 28 are the first mixing assembly 283 and the first mixing assembly 284 respectively. The odd cycles and even cycles form a cycle to improve the analysis efficiency.

[0061] Please continue to refer to Figure 7 , during the odd cycle operation, the work process is as follows:

[0062] Judge whether the incubation tray delivers a cup;

[0063] (1) When the incubation tray delivers a cup, judge whether there is a cup in the first magnetic separation assembly 273. A variable is set for each position of the two magnetic separation assemblies 27 and the two mixing assemblies 28 in the software. According to the process actions, if a cup is placed, it is set to 1, and if the cup is taken away, it is set to 0.

[0064] (11) If there is a cup, the device is in an abnormal state and alarms;

[0065] (12) When there is no cup, the gripper assembly 25 grabs the cup from the incubation tray to the first magnetic separation assembly 273, and then judges whether there is a cup in the second mixing assembly 284.

[0066] (121) When there is a cup, the gripper assembly 25 grabs the cup on the second mixing assembly 284 to the incubation tray, and then judges whether there is a cup in the second magnetic separation assembly 274. (1211) When there is a cup, the gripper assembly 25 grabs the cup to the second mixing assembly 284, and the gripper assembly 25 returns to the origin and stops; (1212) When there is no cup, the gripper assembly 25 stops at the origin.

[0067] (122) When there is no cup, the gripper assembly 25 returns to the origin, and it is determined whether there is a cup in the second magnetic separation assembly 274. (1221) When there is a cup, the gripper assembly 25 grabs the cup in the second magnetic separation assembly 274 and moves it to the second mixing assembly 284, and then the gripper assembly 25 returns to the origin and remains stationary. (1222) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0068] (2) When the incubation tray does not deliver a cup, it is determined whether there is a cup in the second mixing assembly 284.

[0069] (21) When there is a cup, the gripper assembly 25 grabs the cup and moves it to the incubation tray, and then it is determined whether there is a cup in the second magnetic separation assembly 274.

[0070] (211) When there is a cup, the gripper assembly 25 grabs the cup and moves it to the second mixing assembly 284, and then the gripper assembly 25 returns to the origin and remains stationary. (211) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0071] (22) When there is no cup, the gripper assembly 25 returns to the origin, and it is determined whether there is a cup in the second magnetic separation assembly 274.

[0072] (221) When there is a cup, the gripper assembly 25 grabs the cup and moves it to the second mixing assembly 284, and then the gripper assembly 25 returns to the origin and remains stationary. (222) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0073] Please continue to refer to Figure 8 , when operating in an even cycle, the workflow is as follows:

[0074] Determine whether the incubation tray delivers a cup.

[0075] (1) When the incubation tray delivers a cup, it is determined whether there is a cup in the second magnetic separation assembly 274. A variable is set in the software for each position of the two magnetic separation assemblies 27 and the two mixing assemblies 28. According to the process actions, the variable is set to 1 when a cup is placed and set to 0 when the cup is taken away.

[0076] (11) If there is a cup, the device is in an abnormal state and an alarm is issued.

[0077] (12) When there is no cup, the gripper assembly 25 grabs the cup from the incubation tray and moves it to the second magnetic separation assembly 274, and then it is determined whether there is a cup in the first mixing assembly 283.

[0078] (121) When there is a cup, the gripper assembly 25 grabs the cup on the first mixing assembly 283 and moves it to the incubation tray, and then it is determined whether there is a cup in the first magnetic separation assembly 273. (1211) When there is a cup, the gripper assembly 25 grabs the cup and moves it to the first mixing assembly 283, and then the gripper assembly 25 returns to the origin and remains stationary. (1212) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0079] (122) When there is no reaction cup, the gripper assembly 25 returns to the origin, and it is determined whether there is a reaction cup in the first magnetic separation assembly 273. (1221) When there is a reaction cup, the gripper assembly 25 grabs the reaction cup in the first magnetic separation assembly 273 and transports it to the first mixing assembly 283, and then the gripper assembly 25 returns to the origin and remains stationary. (1222) When there is no reaction cup, the gripper assembly 25 remains stationary at the origin.

[0080] (2) When the incubation tray does not transport a reaction cup, it is determined whether there is a reaction cup in the first mixing assembly 283.

[0081] (21) When there is a reaction cup, the gripper assembly 25 grabs the reaction cup and transports it to the incubation tray, and it is determined whether there is a reaction cup in the first magnetic separation assembly 273.

[0082] (211) When there is a reaction cup, the gripper assembly 25 grabs the reaction cup and transports it to the first mixing assembly 283, and then the gripper assembly 25 returns to the origin and remains stationary. (211) When there is no reaction cup, the gripper assembly 25 remains stationary at the origin.

[0083] (22) When there is no reaction cup, the gripper assembly 25 returns to the origin, and it is determined whether there is a reaction cup in the first magnetic separation assembly 273.

[0084] (221) When there is a reaction cup, the gripper assembly 25 grabs the reaction cup and transports it to the first mixing assembly 283, and then the gripper assembly 25 returns to the origin and remains stationary. (222) When there is no reaction cup, the gripper assembly 25 remains stationary at the origin.

[0085] Compared with the prior art, the mounting post 21 of the cleaning and separation device of the present invention includes an end portion 210. The transverse plate 221 is provided with an adjustment hole 2210, and the diameter of the adjustment hole 2210 is larger than the diameter of the end portion 210. The end portion 210 is located in the adjustment hole 2210 and can adjust its position so that the position of the mounting frame 22 relative to the mounting post 21 can be adjusted. The positioning piece 23 fixes the mounting post 21 and the mounting frame 22. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are fixed to the transverse plate 221 and are located on the same straight line, enabling the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 to be integrally installed and disassembled. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are located on a straight line, facilitating the movement of the gripper assembly 25. The cleaning needle 244 is rotatably mounted on the base 242 through a bearing 243, and the cleaning needle 244 is connected to a pipeline. When the pipeline forms a loop, the cleaning needle 244 rotates, which can prevent the pipeline from forming a loop. The four magnets 272 are arranged in a cross shape, and the two magnets 272 located on the same straight line have the same magnetic polarity, enabling the magnets 272 to better adsorb the magnetic beads in the reaction cup 100. The axis of the mixing head 282 forms an angle with the axis of the mixing base 281, so that when the mixing base 281 rotates, the reactants in the reaction cup 100 in the mixing head 282 are mixed more evenly.

[0086] The above embodiments only represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made. These are all equivalent modifications and evolutions made to the above embodiments based on the substantial technology of the present invention, and all of these fall within the protection scope of the present invention.

Claims

1. Cleaning and separating device, including a mounting bracket, characterized in that: The mounting frame includes a vertical plate and a horizontal plate fixed to the vertical plate, and the cleaning and separation device also includes a mounting column, a positioning plate, a cleaning needle assembly, a gripper assembly, a cleaning station assembly, a magnetic separation assembly, a mixing assembly and a translation drive assembly. The translation drive assembly includes a translation drive member, a rotating shaft and a belt. The cleaning needle assembly and the gripper assembly are transmission-connected to the translation drive member. The cleaning station assembly, the magnetic separation assembly and the mixing assembly are fixed to the horizontal plate and are located on the same straight line. The magnetic separation assembly is located between the cleaning station assembly and the mixing assembly. The mounting column includes an end portion, and the horizontal plate is provided with an adjustment hole. The diameter of the adjustment hole is larger than the diameter of the end portion. The end portion is located in the adjustment hole and can adjust its position so that the position of the mounting frame relative to the mounting column can be adjusted. The positioning plate fixes the mounting column and the mounting frame.

2. The cleaning and separating device according to claim 1, wherein: The transverse plate is also provided with a fixing hole, and the positioning plate is provided with a locking hole and a positioning groove. The locking hole is fixed to the end of the mounting column, and the position of the positioning groove corresponds to the fixing hole. The screw passes through the fixing hole and extends into the positioning groove and cooperates with the nut to fix the mounting column and the mounting frame.

3. The cleaning and separating device according to claim 1, wherein: The translational drive member is fixed to the vertical plate, the number of the rotating shafts is multiple, the multiple rotating shafts are rotatably installed on the vertical plate, the belt is sleeved on the rotating shaft, the output end of the translational drive member is fixedly connected to one of the rotating shafts, and the cleaning needle assembly and the gripper assembly are connected to the belt.

4. The cleaning and separating device according to claim 1, wherein: The cleaning needle assembly comprises a base, a bearing and a cleaning needle. The base is mounted on the vertical plate, the bearing is mounted in the base, and the cleaning needle is rotatably mounted on the base via the bearing.

5. The cleaning and separating device according to claim 4, wherein: The cleaning needle assembly also includes a vertical driving member and a rack. A gear is installed at the output end of the vertical driving member. The vertical gear is meshed with the rack to drive the base to move in the vertical direction.

6. The cleaning and separating device according to claim 1, wherein: The magnetic separation assembly comprises a separation base and a magnet. The separation base is fixed to the transverse plate, and the magnet is installed inside the separation base.

7. The cleaning and separating device according to claim 6, wherein: The number of the magnets is four, and the four magnets are arranged in a cross shape. Two magnets located on the same straight line have the same magnetic properties, and a space for accommodating a reaction cup is formed between the four magnets.

8. The cleaning and separating device according to claim 1, wherein: The mixing assembly includes a mixing motor, a mixing base and a mixing head. The mixing motor is fixed to the horizontal plate, the mixing base is installed at the output end of the mixing motor, the mixing motor drives the mixing base to rotate, the mixing head is installed on the mixing base, and the mixing head is used to install a reaction cup.

9. The cleaning and separating device according to claim 8, wherein: The axis of the mixing head forms an angle with the axis of the mixing base.

10. The method for using the cleaning and separating device according to any one of claims 1-9, characterized in that, The following steps are involved: Install the mounting bracket on the mounting column, adjust the position of the mounting bracket, and lock the mounting bracket and the mounting column with the positioning piece; The translation drive assembly drives the gripper assembly to translate to the incubation tray, grabs the reaction cup on the incubation tray and moves the reaction cup to the magnetic separation assembly; The magnet attracts the magnet in the reaction cup, the liquid suction needle of the cleaning needle assembly sucks the liquid in the reaction cup, and the dispensing needle of the cleaning needle assembly adds buffer solution to the reaction cup to achieve the cleaning of the reactants in the reaction cup; The gripper assembly grabs the reaction cup on the magnetic separation assembly to the mixing assembly, the mixing motor drives the mixing base to rotate, and the reactants in the reaction cup are mixed; The gripper assembly grabs the reaction cup on the mixing assembly to the incubation tray, and the incubation tray incubates the reactants in the reaction cup; The cleaning needle assembly completes one cycle of actions and moves to the cleaning station for cleaning.

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