Strain genetic improvement experiment platform and improvement method in industrialized production of pilose antler mushrooms
By designing a genetic improvement experimental platform for plant-based production of deer antler mushrooms, the problems of slow growth rate of deer antler mushrooms and uneven distribution of nutrients were solved, the growth environment of deer antler mushrooms was optimized, and the yield and experiment accuracy were improved.
Patent Information
- Application Number
- CN202410085614.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-22
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2044-01-22
AI Technical Summary
The mycelium of antler mushroom grows slowly, has poor activity, and is difficult to maintain its species, resulting in low factory production yield and uneven distribution of nutrients affecting growth.
A experimental platform for genetic improvement of strains in factory production of deer antler mushrooms was designed. Through structures such as rotating shafts, wedges and extrusions, the nutrients in the test tube are uniformly shaken, combined with constant temperature chambers and gas injection to simulate different environments, and tested the growth status of mycelium.
The uniform distribution of nutrients was achieved, the mycelium growth rate and the scientificity and repeatability of experiments were improved, and the optimal growth conditions were determined.
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Figure CN120345494A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of antler mushroom production, specifically to a strain genetic improvement experimental platform and improvement method in the industrialized production of antler mushrooms. Background Art
[0002] Compared with other fungi with mature cultivation techniques, the mycelial growth rate of antler mushrooms is very slow, and the activity is poor. It is difficult to maintain the varietal characteristics, and the strains degenerate quickly, resulting in a low yield in the industrialized production of antler mushrooms and being difficult to meet the market demand. Therefore, it is necessary to optimize the production technology of antler mushrooms to increase the yield of antler mushrooms.
[0003] During the process of cultivating antler mushrooms, the staff needs to place the nutrients required for cultivating antler mushrooms in a test tube according to a ratio, and then shake it manually. During the shaking process, the nutrients in the test tube are fully mixed, and then the processed antler mushroom mycelia are placed in. The unmixed nutrients are unevenly distributed in the test tube, and the nutrient components required for mycelial growth are also unevenly distributed, which may affect the growth of the mycelia.
[0004] In addition, in order to increase the yield of antler mushrooms, the experimenters need to place multiple portions of antler mushroom mycelia at different environmental temperatures. Different experimental conditions facilitate the staff to confirm the optimal temperature conditions required for the growth of antler mushroom mycelia, thereby increasing the yield of antler mushrooms.
[0005] Therefore, we propose a strain genetic improvement experimental platform and improvement method in the industrialized production of antler mushrooms, which can quickly shake the nutrients in the test tube, test the effects of different proportions of nutrients on the growth of antler mushrooms, and test the growth conditions of antler mushroom mycelia at different temperatures, so as to solve the above problems. Summary of the Invention
[0006] (1) Technical Problems to be Solved
[0007] In view of this, aiming at the deficiencies of the prior art, the present invention provides a strain genetic improvement experimental platform and improvement method in the industrialized production of antler mushrooms to solve the problems raised in the above background art.
[0008] (2) Technical Solutions
[0009] To achieve the purposes of quickly shaking the nutrients inside the test tube, testing the effects of nutrients in different proportions on the growth status of Hericium coralloides, and testing the growth status of Hericium coralloides mycelium at different temperatures, the present invention provides the following technical solutions: A genetic improvement experimental platform and improvement method for Hericium coralloides in factory production, including a bottom plate. At the top of the center position of the bottom plate, a rotating shaft is rotatably connected. One end of the rotating shaft away from the bottom plate is fixedly connected with a wedge block. An outer circumference of the wedge block is provided with a groove sized to fit the extrusion member around the center point of the wedge block. At the top of the center position of the wedge block, a rotating member is fixedly connected. The rotating member is provided with a convex block sized to fit the rotating shaft. At the top of the rotating member, a telescopic rod is rotatably connected. The size of the telescopic rod is adapted to the distance between the rotating shaft and the rotating member. At the top of the telescopic rod, a rotating shaft with its bottom end meshing with the rotating member is provided. At the bottom of the rotating shaft, a groove sized to fit the convex block provided on the rotating member is opened. The rotating shaft is connected to the output shaft of an external power source. An extrusion member is provided around the outside of the wedge block. The size of the extrusion member is adapted to the groove opened on the outside of the wedge block. The extrusion members are arranged in central symmetry with the center point of the wedge block as the center of symmetry. One end of each extrusion member away from the wedge block is rotatably connected with a connecting rod. The size of the connecting rod is adapted to the distance between the wedge block and the positioning block. One end of each connecting rod away from the extrusion member is rotatably connected with a positioning block. The positioning block is internally provided with a through hole sized to fit the test tube. Below one end of the connecting rod close to the extrusion member, a columnar body is provided. An outer side of the columnar body is provided with a limiting block. An elliptical sliding groove is opened at the top of the limiting block. On one side of each limiting block close to the rotating shaft, a fixing member with one end fixedly connected to the outside of the rotating shaft is provided. A spiral groove is opened on the outside of the rotating shaft. The spiral grooves are distributed in central symmetry with the axis of the rotating shaft as the center of symmetry;
[0010] It further includes a placement platform arranged outside the rotating shaft. The placement platform is provided with through holes sized and numbered to fit the positioning rings. Several positioning rings are embedded in the placement platform. The positioning rings are internally provided with grooves sized to fit the elastic connecting members. The positioning rings are distributed in central symmetry with the center point of the placement platform as the center of symmetry. An elastic connecting member is slidably connected inside the positioning ring. One end of the elastic connecting member away from the positioning ring is fixedly connected to a fixing ring arranged inside the positioning ring. The fixing ring is internally provided with a circular hole sized to fit the test tube. A test tube is clamped inside the fixing ring. The bottom of the positioning ring is fixedly connected with symmetrically arranged telescopic members. The size of the telescopic members is adapted to the distance between the positioning ring and the limiting members. One end of each telescopic member away from the test tube is fixedly connected to a limiting member arranged on the outside of the test tube. A through groove with a trapezoidal cross-section is opened inside the limiting member.
[0011] Preferably, one end of the extrusion member away from the wedge block penetrates through the top of the fixing member. A spring is arranged on the outside of the extrusion member. The columnar body is slidably connected in the elliptical sliding groove.
[0012] Preferably, the end of the slider away from the spiral groove is fixedly connected to a driven ring arranged on the outside of the rotating shaft, a circular hole with a size matching the rotating shaft is opened inside the driven ring, a groove with a size matching the rotating block is opened on the outer wall of the driven ring, the outer wall of the driven ring is rotatably connected to the rotating block, and a plurality of connecting rods are fixedly connected to the outside of the rotating block, the connecting rods are centrally symmetrically distributed with the center point of the rotating block as the center of symmetry, and two of the connecting rods axially symmetrically arranged have through holes with a size matching the moving trajectory fixing rod.
[0013] Preferably, one end of the connecting rod away from the rotating block is fixedly connected to a limiting ring, a sliding groove with a size matching the fixed block is opened inside the limiting ring, a through hole with an elliptical cross-section is opened inside the limiting ring, and moving track fixing rods are penetrated inside two of the connecting rods symmetrically arranged along the axis, and the bottom ends of the moving track fixing rods are fixedly connected to the placement platform.
[0014] Preferably, the interior of the limiting ring is rotatably connected with a fixed block, the interior of the fixed block is provided with an air pipe, and the bottom end of the air pipe is fixedly connected with a piston.
[0015] Preferably, the constant temperature chamber is arranged at the bottom of the placement platform, and a heating device is arranged inside the constant temperature chamber.
[0016] The method for improving the strain genetic improvement experimental platform in the factory production of Pleurotus eryngii as mentioned above comprises the following steps:
[0017] Step 1: Culture medium preparation:
[0018] Step 1.1: Get bran, poplar sawdust, bean dregs, yield increasing agent, superphosphate, humic acid and distilled water in proportion and mix them thoroughly;
[0019] Step 1.2: Put the mixture obtained in step 1.1 into the test tube, start the external motor to rotate the rotating shaft, and indirectly make the positioning block shake the mixture in the test tube, and the culture medium preparation is completed;
[0020] Step 2: Test the environment required for growth:
[0021] Step 2.1: Add an equal amount of Pleurotus eryngii mycelium into the test tube treated in step 1;
[0022] Step 2.2: Injecting mixed gases of different proportions and types into different test tubes in step 2.1;
[0023] Step 2.3: placing the mycelium treated in step 2.2 in an outdoor environment at different temperatures and maintaining the temperature constant, wherein the external environment temperature ranges between 20°C and 25°C;
[0024] Step 3. Observe the growth status of Hericium coralloides:
[0025] Step 3.1: Observe and record the growth status of Hericium coralloides mycelium in different test tubes after the treatment in Step 2 within 60 days.
[0026] Preferably, in Step 1.1, there are 20 - 30 parts of wheat bran, 20 - 30 parts of poplar sawdust, 4 - 5 parts of soybean dregs, 4 - 5 parts of growth promoter, 1 - 2 parts of superphosphate, 1 - 2 parts of humic acid, and 20 - 30 parts of distilled water.
[0027] Preferably, for the gas injected into the test tubes in Step 2.2, two or three of carbon dioxide, nitrogen, methane, and oxygen are selected.
[0028] (III) Beneficial effects
[0029] Compared with the prior art, the present invention provides an experimental platform and improvement method for genetic improvement of strains in the industrial production of Hericium coralloides, having the following beneficial effects:
[0030] 1. Through the multiple test tubes set, experimenters can place the culture medium suitable for the growth of Hericium coralloides mycelium according to the combination of different materials and different ratios of the same material, so as to observe the growth status of multiple groups of Hericium coralloides mycelium at one time. In this process, experimenters have flexible operation space and can place the prepared culture medium into different test tubes according to the combination of different materials and different ratios of the same material, so as to observe the growth status of multiple groups of Hericium coralloides mycelium at one time. Thereby further optimizing the growth rate of Hericium coralloides mycelium.
[0031] 2. By evenly swinging the nutrients put into the test tubes to make them evenly distributed inside the test tubes, while avoiding manual shaking of multiple groups of test tubes, it can also quickly and fully mix the paired substances inside the test tubes, so that the mycelium will not have problems in growth status due to uneven distribution of nutrients during the growth process, thus achieving the purpose of obtaining accurate test results.
[0032] 3. By injecting different types of gases into the test tubes for culturing Hericium coralloides mycelium, we can observe the growth status of the mycelium in different gas environments, and can have a more detailed and accurate understanding of the growth rate of the mycelium in different gas environments. In different gas environments, the stress resistance of Hericium coralloides mycelium can be tested, such as its growth requirements for gases such as carbon dioxide, nitrogen, methane, and oxygen, as well as its adaptability to acidity and alkalinity, thus ensuring the repeatability and scientificity of the experiment.
[0033] 4. By changing the external temperature of the test tube, the growth status of the mycelium of Hericium coralloides can be tested at different ambient temperatures, so as to determine the optimal temperature required for the growth of Hericium coralloides mycelium, thereby achieving the purpose of testing the growth status of Hericium coralloides mycelium at different temperatures. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] Figure 1 It is a schematic diagram of the overall appearance of the present invention;
[0035] Figure 2 It is a schematic diagram of the connection relationship at the limiting block of the present invention;
[0036] Figure 3 It is a schematic diagram of the connection relationship at the rotating shaft of the present invention;
[0037] Figure 4 For the present invention Figure 3 The enlarged view of the structure at A in the present invention;
[0038] Figure 5 It is a schematic diagram of the connection relationship at the placement platform of the present invention;
[0039] Figure 6 It is a schematic diagram of the positional relationship at the test tube of the present invention;
[0040] Figure 7 It is a schematic diagram of the positional relationship at the wedge block of the present invention;
[0041] Figure 8 It is a schematic diagram of the connection relationship at the columnar body of the present invention;
[0042] Figure 9 It is a schematic diagram of the process flow of the genetic improvement method of the Hericium coralloides strain of the present invention.
[0043] In the figure: 101, bottom plate; 102, rotating shaft; 103, wedge block; 104, rotating part; 105, telescopic rod; 106, rotating shaft; 107, extrusion part; 108, connecting rod; 109, positioning block; 110, columnar body; 111, limiting block; 112, elliptical chute; 113, fixing part; 114, spiral groove;
[0044] 201, placement platform; 202, positioning ring; 203, elastic connecting piece; 204, fixing ring; 205, test tube; 206, telescopic piece; 207, limiting piece;
[0045] 301, slider; 302, driven ring; 303, rotating block; 304, connecting rod; 305, limiting ring; 306, moving track fixing rod; 307, fixing block; 308, air pipe; 309, piston;
[0046] 401, constant temperature chamber. DETAILED DESCRIPTION OF THE INVENTION
[0047] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.
[0048] Embodiment
[0049] Please refer to Figures 1 to 8, A strain genetic improvement experimental platform and improvement method in the industrialized production of Hericium coralloides, including a bottom plate 101. At the top of the center position of the bottom plate 101, a rotating shaft 102 is rotatably connected. One end of the rotating shaft 102 away from the bottom plate 101 is fixedly connected with a wedge block 103. A groove adapted to the size of the pressing member 107 is opened around the center point of the wedge block 103 outside the wedge block 103. At the top of the center position of the wedge block 103, a rotating member 104 is fixedly connected. A convex block adapted to the size of the rotating shaft 106 is opened on the rotating member 104. The top of the rotating member 104 is rotatably connected with a telescopic rod 105. The size of the telescopic rod 105 is adapted to the distance between the rotating shaft 106 and the rotating member 104. At the top of the telescopic rod 105, there is a rotating shaft 106 whose bottom end meshes with the rotating member 104. A groove adapted to the convex block provided on the rotating member 104 is opened at the bottom of the rotating shaft 106. The rotating shaft 106 is connected to the output shaft of an external power source. An extrusion member 107 is arranged around the outside of the wedge block 103. One end of the extrusion member 107 away from the wedge block 103 penetrates through the top of the fixing member 113. A spring is arranged on the outside of the extrusion member 107. The size of the extrusion member 107 is adapted to the groove opened on the outside of the wedge block 103. The extrusion members 107 are centrally symmetrically arranged with the center point of the wedge block 103 as the center of symmetry. A connecting rod 108 is rotatably connected to each side of the extrusion member 107 away from the wedge block 103. The size of the connecting rod 108 is adapted to the distance between the wedge block 103 and the positioning block 109. One end of the connecting rod 108 away from the extrusion member 107 is rotatably connected to a positioning block 109. A through hole adapted to the size of the test tube 205 is opened inside the positioning block 109. Below one end of the connecting rod 108 close to the extrusion member 107, there is a columnar body 110. The columnar body 110 is slidably connected in the elliptical chute 112. A limiting block 111 is arranged on the outside of the columnar body 110. An elliptical chute 112 is opened at the top of the limiting block 111. On one side of the limiting block 111 close to the rotating shaft 102, there is a fixing member 113 whose one end is fixedly connected to the outside of the rotating shaft 102. A spiral groove 114 is opened on the outside of the rotating shaft 106. The spiral grooves 114 are centrally symmetrically distributed with the axis of the rotating shaft 106 as the center of symmetry. It also includes a placement platform 201 arranged outside the rotating shaft 106. Through holes adapted to the size and quantity of the positioning rings 202 are opened on the placement platform 201. Several positioning rings 202 are embedded on the placement platform 201. A groove adapted to the size of the elastic connecting member 203 is opened inside the positioning ring 202. The positioning rings 202 are centrally symmetrically arranged with the center point of the placement platform 201 as the center of symmetry. An elastic connecting member 203 is slidably connected inside the positioning ring 202. One end of the elastic connecting member 203 away from the positioning ring 202 is fixedly connected to a fixing ring 204 arranged inside the positioning ring 202. A circular hole adapted to the size of the test tube 205 is opened inside the fixing ring 204. A test tube 205 is clamped inside the fixing ring 204. The bottom of the positioning ring 202 is fixedly connected with a symmetrically arranged telescopic member 206,The size of the telescopic member 206 is adapted to the distance between the positioning ring 202 and the limiting member 207. One end of the telescopic member 206 away from the test tube 205 is fixedly connected to the limiting member 207 provided on the outer side of the test tube 205. A through groove with a trapezoidal cross-section is formed inside the limiting member 207.
[0050] The effects achieved by this embodiment are as follows: Compared with the prior art, this device can shake multiple groups of nutrients, enabling the nutrients inside the test tube 205 to be fully mixed, ensuring the growth environment of the Hericium coralloides mycelium. At the same time, the multiple test tubes 205 are convenient for experimenters to conduct multiple groups of experiments at one time, so as to measure the optimal ratio and required nutrients for the growth of the Hericium coralloides mycelium.
[0051] Further embodiment
[0052] Please refer to Figures 1 to 6 , a strain genetic improvement experimental platform and improvement method in the industrialized production of Hericium coralloides, including a slider 301 slidably connected inside a spiral groove 114. One end of the slider 301 away from the spiral groove 114 is fixedly connected to a driven ring 302 provided on the outer side of a rotating shaft 106. A circular hole adapted to the size of the rotating shaft 106 is formed inside the driven ring 302. A groove adapted to the size of a rotating block 303 is formed on the outer wall of the driven ring 302. A rotating block 303 is rotatably connected to the outer wall of the driven ring 302. A plurality of connecting rods 304 are fixedly connected to the outer side of the rotating block 303. The connecting rods 304 are centrally symmetrically distributed with the center point of the rotating block 303 as the center of symmetry. Through holes adapted to the size of a moving track fixing rod 306 are formed in two axially symmetrically arranged connecting rods 304. One end of each connecting rod 304 away from the rotating block 303 is fixedly connected to a limiting ring 305. A sliding groove adapted to the size of a fixing block 307 is formed inside the limiting ring 305. An elliptical through hole is formed inside the limiting ring 305. A moving track fixing rod 306 is penetrated through the interiors of two axially symmetrically arranged connecting rods 304. The bottom end of the moving track fixing rod 306 is fixedly connected to a placement platform 201. Fixing blocks 307 are rotatably connected inside the limiting rings 305. Air pipes 308 are arranged inside the fixing blocks 307. The air pipes 308 are connected to the gas output ports of an external gas storage device. Pistons 309 are fixedly connected to the bottom ends of the air pipes 308. A constant temperature chamber 401 is further provided on the outer side of the test tube 205. The constant temperature chambers 401 are all arranged at the bottom of the placement platform 201. A heating device is arranged inside the constant temperature chamber 401.
[0053] The effects achieved by this embodiment are as follows: compared with the prior art, the device places the mycelium of Pleurotus eryngii in different gas environments, so as to test the influence of different types of gas environments on the growth of mycelium of Pleurotus eryngii. At the same time, the growth conditions of mycelium under different temperature environments can also be tested, so as to obtain the optimal gas environment and temperature suitable for the growth of mycelium of Pleurotus eryngii.
[0054] Further Embodiments
[0055] The method for improving the strain legacy experimental platform in the above-mentioned factory production of Pleurotus eryngii comprises the following steps:
[0056] Step 1: Culture medium preparation:
[0057] Step 1.1: Get bran, poplar sawdust, bean dregs, yield increasing agent, superphosphate, humic acid and distilled water in proportion and mix them thoroughly;
[0058] Step 1.2: Put the mixture obtained in step 1.1 into the test tube 205, start the external motor to rotate the rotating shaft 106, and indirectly make the positioning block 109 shake the mixture in the test tube 205, and the culture medium is prepared;
[0059] Step 2: Test the environment required for growth:
[0060] Step 2.1: Adding an equal amount of Pleurotus eryngii mycelium into the test tube 205 processed in step 1;
[0061] Step 2.2: Injecting mixed gases of different proportions and types into different test tubes 205 in step 2.1;
[0062] Step 2.3: placing the mycelium treated in step 2.2 in an outdoor environment at different temperatures and maintaining the temperature constant, wherein the external environment temperature ranges between 20°C and 25°C;
[0063] Step 3: Observe the growth of velvet antler mushroom:
[0064] Step 3.1: Observe and record the growth conditions of the mycelium of Pleurotus eryngii in different test tubes 205 after the treatment in step 2 within 60 days.
[0065] In step S1.1, 25 parts of bran, 25 parts of poplar sawdust, 4 parts of bean dregs, 4 parts of yield increasing agent, 1 part of superphosphate, 1 part of humic acid, and 25 parts of distilled water are included.
[0066] In step S2.2, the gas injected into the test tube is selected from two or three of carbon dioxide, nitrogen, methane and oxygen.
[0067] The working process and principle of the overall content of the above embodiment are as follows:
[0068] First, nutrients with different ratios are separately put into different test tubes 205, and marks are made on the test tubes 205. Subsequently, the test tubes 205 are placed inside the positioning ring 202, and the positioning ring 202 fixes the positions of the test tubes 205.
[0069] Subsequently, the staff starts the external motor, and the rotating shaft 106 connected to the power output shaft of the external motor will rotate under the action of the external motor. At this time, the spiral groove 114 slidably connected to the outside of the rotating shaft 106 will move accordingly, prompting the slider 301 arranged inside the spiral groove 114 to spiral downward along the track of the spiral groove 114, and the driven ring 302 fixedly connected to the slider 301 will also move accordingly.
[0070] Since the rotating block 303 is rotatably connected to the outer wall of the driven ring 302, and the connecting rod 304 is fixedly connected to the outside of the rotating block 303, the rotating block 303 and the connecting rod 304 will move along with the driven ring 302. During the movement of the connecting rod 304, under the action of the moving track fixing rod 306 arranged inside it, it will move vertically downward, prompting the limiting ring 305 fixedly connected to the other end of the connecting rod 304 to move synchronously. At this time, the fixing block 307 arranged inside the limiting ring 305, the air pipe 308 arranged inside the fixing block 307, and the piston 309 connected to the air pipe 308 will move downward accordingly, and the piston 309 will move towards the test tube 205 until it is inserted into the test tube 205.
[0071] As the rotating shaft 106 rotates, the slider 301 will drive the driven ring 302 to gradually move to the end of the spiral groove 114. During this process, the piston 309 gradually inserted into the test tube 205 will drive the test tube 205 to move downward synchronously under the action of friction, so that the fixing ring 204 arranged outside the test tube 205, through the elastic connecting piece 203 and the positioning ring 202, prompts the placement platform 201 arranged outside the positioning ring 202 to move downward synchronously.
[0072] The movement of the placement platform 201 will cause the rotating shaft 106 connected to it to move downward. While compressing the telescopic rod 105, the rotating shaft 106 will gradually move towards the rotating part 104 until it meshes with the rotating part 104. During this process, the position of the test tube 205 will also decrease as the placement platform 201 descends. When the rotating shaft 106 meshes with the rotating part 104, the bottom of the test tube 205 will be embedded into the positioning block 109.
[0073] When the rotating shaft 106 and the rotating part 104 are in the meshing state, they will rotate synchronously under the action of the external motor. At this time, the wedge block 103 fixedly connected to the rotating part 104 will rotate accordingly, causing the extrusion part 107 that fits against the outer wall of the wedge block 103 to perform a reciprocating motion of approaching and separating from the wedge block 103 under the action of the wedge block 103. While the extrusion part 107 is moving, the positioning block 109 connected to the extrusion part 107 through the connecting rod 108 will also move accordingly. During the movement process, it will perform a reciprocating motion with an elliptical trajectory under the action of the cylindrical body 110 provided at the bottom of the connecting rod 108 and the elliptical groove provided on the limiting block 111.
[0074] At this time, the bottom of the test tube 205 clamped inside the positioning block 109 will move synchronously under the action of the positioning block 109. During this process, since a fixing ring 204 is provided on the outer side of the top of the test tube 205 and an elastic connecting piece 203 is provided on the outer side of the fixing ring 204, when the bottom of the test tube 205 rotates reciprocally, the top of the test tube 205 will also swing, making it evenly distributed inside the test tube 205. While avoiding manual shaking of multiple groups of test tubes 205, it can also quickly make the paired substances inside the test tube 205 fully mixed, so that the hyphae will not have problems in the growth process due to uneven distribution of nutrients, thus achieving the purpose of shaking the nutrients inside the test tube 205.
[0075] After the test tube 205 is shaken evenly, the external motor causes the rotating shaft 106 to rotate in the reverse direction. The rotating shaft 106 will cause the slider 301 to move in the reverse direction, and other structures will also move in the reverse direction according to the above steps, causing the piston 309 to disengage from the test tube 205.
[0076] Subsequently, the staff puts the antler mushroom hyphae into the test tube 205, and then starts the external motor again to make the rotating shaft 106 rotate forward. After moving according to the above movement process, the piston 309 will re-engage into the test tube 205. At this time, the rotating shaft 106 is notified to rotate.
[0077] Subsequently, the staff injects different types of gases into different test tubes 205 through the air pipe 308 to simulate the growth conditions of the antler mushroom hyphae in different gas environments. In different gas environments, the stress resistance of the antler mushroom hyphae can be tested, such as its growth requirements for gases such as carbon dioxide, nitrogen, methane, oxygen, etc., and its adaptability to the pH value, so as to ensure the repeatability and scientificity of the experiment.
[0078] During the test process, the constant temperature chamber 401 can also be adjusted to different temperatures to test the growth conditions of the antler mushroom hyphae under different temperature conditions, so as to determine the optimal temperature required for the growth of the antler mushroom hyphae.
[0079] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the phrase "comprising an..." does not exclude the presence of additional identical elements in the process, method, article or device comprising said element.
[0080] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An experimental platform for genetic improvement of strains in the industrialized production of velvet antler mushrooms, including a bottom plate (101), characterized in that: At the top of the center position of the bottom plate (101), a rotating shaft (102) is rotatably connected. One end of the rotating shaft (102) away from the bottom plate (101) is fixedly connected with a wedge block (103). At the top of the center position of the wedge block (103), a rotating part (104) is fixedly connected. At the top of the rotating part (104), a telescopic rod (105) is rotatably connected. At the top of the telescopic rod (105), a rotating shaft (106) with the bottom end meshing with the rotating part (104) is provided. Outside the wedge block (103), an extrusion part (107) is arranged around the wedge block (103). The extrusion parts (107) are arranged in central symmetry with the center point of the wedge block (103) as the symmetry center. One side of the extrusion part (107) away from the wedge block (103) is rotatably connected with a connecting rod (108). One end of the connecting rod (108) away from the extrusion part (107) is rotatably connected with a positioning block (109). Below one end of the connecting rod (108) close to the extrusion part (107), a columnar body (110) is provided. Outside the columnar body (110), a limiting block (111) is provided. On the top of the limiting block (111), an elliptical chute (112) is opened. On one side of the limiting block (111) close to the rotating shaft (102), a fixing part (113) with one end fixedly connected to the outside of the rotating shaft (102) is provided. On the outside of the rotating shaft (106), a spiral groove (114) is opened. The spiral grooves (114) are distributed in central symmetry with the axis of the rotating shaft (106) as the symmetry center; It also includes a placement platform (201) arranged outside the rotating shaft (106). Several positioning rings (202) are embedded on the placement platform (201). The positioning rings (202) are distributed in central symmetry with the center point of the placement platform (201) as the symmetry center. Inside the positioning rings (202), an elastic connecting part (203) is slidably connected. One end of the elastic connecting part (203) away from the positioning ring (202) is fixedly connected with a fixing ring (204) arranged inside the positioning ring (202). Inside the fixing ring (204), a test tube (205) is clamped. At the bottom of the positioning ring (202), a telescopic part (206) arranged in axial symmetry is fixedly connected. One end of the telescopic part (206) away from the test tube (205) is fixedly connected to a limiting part (207) arranged outside the test tube (205). Inside the limiting part (207), a through groove with a trapezoidal cross-section is opened.
2. The strain genetic improvement experimental platform in the industrialized production of Hericium coralloides according to claim 1, characterized in that: One end of the extrusion part (107) away from the wedge block (103) penetrates through the top of the fixing part (113). A spring is arranged outside the extrusion part (107). The columnar body (110) is slidably connected in the elliptical chute (112).
3. The strain genetic improvement experimental platform in the industrialized production of Hericium coralloides according to claim 1, characterized in that: The strain genetic improvement experimental platform for the industrial production of Pleurotus eryngii also includes a slider (301) slidably connected to the inside of the spiral groove (114); one end of the slider (301) away from the spiral groove (114) is fixedly connected to a driven ring (302) arranged on the outside of the rotating shaft (106); the outer wall of the driven ring (302) is rotatably connected to a rotating block (303); the outer side of the rotating block (303) is fixedly connected to a plurality of connecting rods (304); the connecting rods (304) are centrally symmetrically distributed with the center point of the rotating block (303) as the symmetry center.
4. The strain genetic improvement experimental platform in the industrialized production of Hericium caput-medusae, as described in claim 5, is characterized in that: One end of the connecting rod (304) away from the rotating block (303) is fixedly connected to a limit ring (305), and a through hole with an elliptical cross-section is provided inside the limit ring (305). A moving track fixing rod (306) is provided inside two connecting rods (304) arranged axially symmetrically, and the bottom end of the moving track fixing rod (306) is fixedly connected to the placement platform (201).
5. The strain genetic improvement experimental platform in the industrialized production of Hericium coralloides according to claim 6, characterized in that: The interior of the limiting ring (305) is rotatably connected to a fixed block (307), the interior of the fixed block (307) is provided with an air pipe (308), and the bottom end of the air pipe (308) is fixedly connected to a piston (309).
6. The experimental platform for genetic improvement of strains in the industrialized production of Hericium coralloides according to claim 1, characterized in that: The strain genetic improvement experimental platform in the industrial production of Pleurotus eryngii also includes a constant temperature chamber (401) arranged outside the test tube (205), and the constant temperature chamber (401) is arranged at the bottom of the placement platform (201).
7. The improvement method of the strain genetic improvement experimental platform in the industrialized production of Hericium coralloides, as claimed in claims 1-6, is characterized in that The following steps are involved: Step 1: Culture medium preparation: Step 1.1: Get bran, poplar sawdust, bean dregs, yield increasing agent, superphosphate, humic acid and distilled water in proportion and mix them thoroughly; Step 1.2: Put the mixture obtained in step 1.1 into the test tube (205), start the external motor to rotate the rotating shaft (106), and indirectly make the positioning block (109) shake the mixture in the test tube (205), and the culture medium is prepared; Step 2: Test the environment required for growth: Step 2.1: Adding an equal amount of Pleurotus eryngii mycelium into the test tube (205) treated in step 1; Step 2.2: injecting mixed gases of different proportions and types into different test tubes (205) in step 2.1; Step 2.3: placing the mycelium treated in step 2.2 in an outdoor environment at different temperatures and maintaining the temperature constant, wherein the external environment temperature ranges between 20°C and 25°C; Step 3: Observe the growth of velvet antler mushroom: Step 3.1: Observe and record the growth conditions of Pleurotus eryngii mycelium in different test tubes (205) after treatment in step 2 within 60 days.
8. The improvement method of the experimental platform for genetic improvement of strains according to claim 8, characterized in that, The proportions in step 1.1 are: 20-30 parts of bran, 20-30 parts of poplar sawdust, 4-5 parts of bean dregs, 4-5 parts of yield-increasing agent, 1-2 parts of superphosphate, 1-2 parts of humic acid, and 20-30 parts of distilled water.
9. The improvement method of the experimental platform for genetic improvement of strains according to claim 8, wherein, In step 2.2, the gas injected into the test tube (205) is selected from two or three of carbon dioxide, nitrogen, methane and oxygen.
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