Genetic improvement experiment platform and method for fungus strain in factory production of pilose antler mushroom

Abstract

The application discloses a genetic improvement experimental platform and method for a fungus in factory production of pilose antler mushroom, relates to the technical field of pilose antler mushroom production, and comprises a bottom plate, a rotating shaft rotatably connected to the top of the center position of the bottom plate, a wedge fixedly connected to the end of the rotating shaft away from the bottom plate, a groove with a size matched with that of an extruding piece and arranged around the center point of the wedge on the outside of the wedge, a rotating piece fixedly connected to the top of the center position of the wedge, a protrusion with a size matched with that of a rotating shaft and arranged on the rotating piece, and an extension rod rotatably connected to the top of the rotating piece. The nutrient substance inside the test tube is uniformly shaken to be evenly distributed in the test tube, the nutrient substance inside the test tube is quickly and fully mixed, the mycelium does not appear to have growth problems due to uneven distribution of the nutrient substance in the growth process, and thus accurate test results are obtained.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pilose antler mushroom production, in particular to a genetic improvement experiment platform and method for strains in the factory production of pilose antler mushrooms. BACKGROUND

[0002] Compared with other maturely cultured fungi, the mycelial growth rate of pilose antler mushrooms is very slow, and the activity is poor, the strain characteristics are difficult to maintain, and the strain degenerates quickly, resulting in low yield of pilose antler mushrooms in factory production, which is difficult to meet market demand. Therefore, it is necessary to optimize the production technology of pilose antler mushrooms and improve the yield of pilose antler mushrooms.

[0003] In the process of cultivating pilose antler mushrooms, the workers need to place the nutrients required for cultivating pilose antler mushrooms in the test tube according to the proportion, and then shake manually. In the process of shaking, the nutrients in the test tube are fully mixed, and then the treated pilose antler mushroom mycelium is placed in. The nutrients in the test tube are not evenly distributed without shaking, and the nutrients required for the growth of mycelium are also not evenly distributed, which may affect the growth of mycelium.

[0004] In addition, in order to improve the yield of pilose antler mushrooms, experimenters need to place multiple pilose antler mushroom mycelia in different environmental temperatures, so as to facilitate workers to confirm the optimal temperature conditions required for the growth of pilose antler mushroom mycelium, thereby improving the yield of pilose antler mushrooms.

[0005] To this end, we propose a genetic improvement experiment platform and method for strains in the factory production of pilose antler mushrooms, which quickly shakes the nutrients in the test tube, tests the influence of different proportions of nutrients on the growth of pilose antler mushrooms, and tests the growth of pilose antler mushroom mycelium under different temperatures, to solve the above problems. SUMMARY

[0006] (I) Technical problems solved

[0007] Therefore, in view of the deficiencies in the prior art, the present application provides a genetic improvement experiment platform and method for strains in the factory production of pilose antler mushrooms to solve the problems proposed in the background.

[0008] (II) Technical solutions

[0009] In order to realize the above-mentioned rapid shaking of the internal nutrients of the test tube, test the influence of different proportions of nutrients on the growth condition of pilose antler mushroom and test the growth condition of pilose antler mushroom mycelium under different temperatures, the present application provides the following technical scheme: a strain genetic improvement experiment platform and method in pilose antler mushroom factory production, comprising a bottom plate, a rotating shaft is rotatably connected to the top of the center position of the bottom plate, a wedge block is fixedly connected to the end of the rotating shaft away from the bottom plate, a groove with a size matched with the extrusion piece is formed around the center point of the wedge block, a rotating piece is fixedly connected to the top of the center position of the wedge block, a protrusion with a size matched with the rotating shaft is formed in the rotating piece, a telescopic rod is rotatably connected to the top of the rotating piece, the size of the telescopic rod is matched with the distance between the rotating shaft and the rotating piece, a rotating shaft with a bottom end engaged with the rotating piece is arranged on the top of the telescopic rod, a groove with a size matched with the protrusion arranged on the rotating piece is formed in the bottom of the rotating shaft, the rotating shaft is connected with the output shaft of an external power supply, an extrusion piece is arranged around the wedge block on the outside of the wedge block, the size of the extrusion piece is matched with the groove formed on the outside of the wedge block, the extrusion piece is centrally symmetrically arranged with the center point of the wedge block as the center of symmetry, a connecting rod is rotatably connected to the side of the extrusion piece away from the wedge block, the size of the connecting rod is matched with the distance between the wedge block and the positioning block, a positioning block is rotatably connected to the end of the connecting rod away from the extrusion piece, a through hole with a size matched with the test tube is formed in the positioning block, a columnar body is arranged below the end of the connecting rod close to the extrusion piece, a limiting block is arranged on the outside of the columnar body, an oval-shaped sliding groove is formed in the top of the limiting block, a fixed piece fixedly connected to the outside of the rotating shaft is arranged on the side of the limiting block close to the rotating shaft, a spiral-shaped groove is formed in the outside of the rotating shaft, the spiral-shaped groove is centrally symmetrically distributed with the axis of the rotating shaft as the center of symmetry.

[0010] Further comprising a placing platform arranged on the outside of the rotating shaft, a through hole with a size and number matched with the positioning ring is formed in the placing platform, a plurality of positioning rings are embedded in the placing platform, a groove with a size matched with the elastic connecting piece is formed in the inside of the positioning ring, the positioning ring is centrally symmetrically distributed with the center point of the placing platform as the center of symmetry, the elastic connecting piece is slidably connected to the inside of the positioning ring, a fixed ring arranged in the inside of the positioning ring is fixedly connected to the end of the elastic connecting piece away from the positioning ring, a circular hole with a size matched with the test tube is formed in the inside of the fixed ring, the test tube is clamped in the inside of the fixed ring, the bottom of the positioning ring is fixedly connected with the telescopic piece arranged in an axisymmetric manner, the size of the telescopic piece is matched with the distance between the positioning ring and the limiting piece, the end of the telescopic piece away from the test tube is fixedly connected to the limiting piece arranged on the outside of the test tube, a through groove with a trapezoidal cross section is formed in the inside of the limiting piece.

[0011] Preferably, the end of the extrusion piece away from the wedge block penetrates through the top of the fixed piece, a spring is arranged on the outside of the extrusion piece, and the columnar body is slidably connected in the oval-shaped sliding groove.

[0012] Preferably, one end of the sliding block away from the spiral groove is fixedly connected with a driven ring arranged outside the rotating shaft, the inside of the driven ring is provided with a circular hole with a size matched with the rotating shaft, the outer wall of the driven ring is provided with a groove with a size matched with the rotating block, the outer wall of the driven ring is rotatably connected with the rotating block, the outside of the rotating block is fixedly connected with a plurality of connecting rods, the connecting rods are centrally symmetrically distributed with the center point of the rotating block as the center of symmetry, and the inside of two axially symmetrically arranged connecting rods is provided with a through hole with a size matched with the moving track fixing rod.

[0013] Preferably, the end of the connecting rod away from the rotating block is fixedly connected with a limiting ring, the inside of the limiting ring is provided with a sliding groove with a size matched with the fixed block, the inside of the limiting ring is provided with a through hole with an elliptical cross section, and the inside of the two axially symmetrically arranged connecting rods is penetrated by a moving track fixing rod.

[0014] Preferably, the inside of the limiting ring is rotatably connected with the fixed block, and the inside of the fixed block is provided with an air pipe, and the bottom end of the air pipe is fixedly connected with a piston.

[0015] Preferably, the constant temperature room is arranged at the bottom of the placing platform, and the inside of the constant temperature room is provided with a heating device.

[0016] The improved method of the above-mentioned experimental platform for genetic improvement of the strain in the factory production of pilose antler mushrooms comprises the following steps:

[0017] Step 1: preparation of culture medium

[0018] Step 1.1: obtain bran, poplar sawdust, bean dregs, yield enhancer, superphosphoric acid calcium, humic acid and distilled water in proportion, and mix them thoroughly;

[0019] Step 1.2: put the mixture obtained in step 1.1 into the test tube, start the external motor, make the rotating shaft rotate, and indirectly make the positioning block shake the mixture in the test tube, and the preparation of the culture medium is completed;

[0020] Step 2: test of environment required for growth

[0021] Step 2.1: put an equal amount of pilose antler mushroom mycelium into the test tube treated in step 1;

[0022] Step 2.2: inject different proportions and types of mixed gas into different test tubes in step 2.1;

[0023] Step 2.3: place the mycelium treated in step 2.2 in an outdoor environment with different temperatures, and keep the temperature constant, wherein the temperature range of the external environment is between 20℃ and 25℃.

[0024] Step 3, observe the growth of deer mushroom:

[0025] Step 3.1: Observe and record the growth of deer mushroom mycelium in different test tubes after step 2 treatment within 60 days.

[0026] Preferably, the bran in step 1.1 is 20-30 parts, poplar sawdust is 20-30 parts, bean dregs are 4-5 parts, yield enhancer is 4-5 parts, superphosphate is 1-2 parts, humic acid is 1-2 parts, and distilled water is 20-30 parts.

[0027] Preferably, the gas injected into the test tube in step 2.2 is selected from two or three of carbon dioxide, nitrogen, methane and oxygen.

[0028] (Three) beneficial effects

[0029] Compared with the prior art, the present application provides a strain genetic improvement experimental platform and method in the factory production of deer mushroom, which has the following beneficial effects:

[0030] 1. By setting multiple test tubes, experimenters can place culture media suitable for deer mushroom mycelium growth in them according to different materials and different proportions of the same material, so as to observe the growth of multiple groups of deer mushroom mycelium at one time. In this link, experimenters have flexible operation space and can place culture media configured according to requirements into different test tubes according to different materials and different proportions of the same material, so as to observe the growth of multiple groups of deer mushroom mycelium at one time. Thus, the growth rate of deer mushroom mycelium is further optimized.

[0031] 2. By uniformly swinging the nutrients inside the test tube, the nutrients are evenly distributed inside the test tube, avoiding manual shaking of multiple test tubes, and also quickly mixing the nutrients inside the test tube, so that the mycelium does not appear problems in growth due to uneven distribution of nutrients during growth, so as to obtain accurate test results.

[0032] 3. By injecting different types of gas into the test tube for cultivating deer mushroom mycelium, we can observe the growth of mycelium in different gas environments and have a more detailed and accurate understanding of the growth rate of mycelium in different gas environments. In different gas environments, the stress resistance of deer mushroom mycelium can be tested, such as its growth requirements for carbon dioxide, nitrogen, methane and oxygen, and its adaptability to pH, so as to ensure the repeatability and scientificity of the experiment.

[0033] 4, By changing the temperature of the external environment where the test tube is located, the growth condition of the pilose antler mushroom mycelium under different environmental temperatures can be tested, so as to determine the optimum temperature required for the growth of the pilose antler mushroom mycelium, thereby achieving the purpose of testing the growth condition of the pilose antler mushroom mycelium under different temperatures. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 It is a schematic diagram of the overall appearance of the application;

[0035] Figure 2 It is a schematic diagram of the connection relationship at the limiting block of the application;

[0036] Figure 3 It is a schematic diagram of the connection relationship at the rotating shaft of the application;

[0037] Figure 4 It is a schematic diagram of the connection relationship at the rotating shaft of the application; Figure 3 It is an enlarged view of structure A in the application;

[0038] Figure 5 It is a schematic diagram of the connection relationship at the placing platform of the application;

[0039] Figure 6 It is a schematic diagram of the position relationship at the test tube of the application;

[0040] Figure 7 It is a schematic diagram of the position relationship at the wedge block of the application;

[0041] Figure 8 It is a schematic diagram of the connection relationship at the columnar body of the application;

[0042] Figure 9 It is a schematic diagram of the pilose antler mushroom genetic improvement method flow of the application.

[0043] In the figure: 101, base plate;102, rotating shaft;103, wedge block;104, rotating part;105, telescopic rod;106, rotating shaft;107, extrusion part;108, connecting rod;109, positioning block;110, columnar body;111, limiting block;112, oval-shaped sliding groove;113, fixed part;114, spiral-shaped groove;

[0044] 201, placing platform;202, positioning ring;203, elastic connecting part;204, fixed ring;205, test tube;206, telescopic part;207, limiting part;

[0045] 301, sliding block;302, driven ring;303, rotating block;304, connecting rod;305, limiting ring;306, moving track fixing rod;307, fixed block;308, air pipe;309, piston;

[0046] 401, constant temperature chamber. DETAILED DESCRIPTION

[0047] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work are within the protection scope of the present application.

[0048] Embodiments

[0049] Please refer to Figures 1 to 8The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. 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The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. 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The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. 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The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antler mushroom. The application discloses a genetic improvement experiment platform and method for a fungus strain in a factory production of pilose antler mushroom, and belongs to the technical field of genetic improvement of a fungus strain in a factory production of pilose antlerThe telescopic piece 206 is fixedly connected to the limiting piece 207 arranged outside the test tube 205, and the limiting piece 207 is internally provided with a through slot with a trapezoidal cross section.

[0050] The effects of the embodiment are as follows: compared with the prior art, the device can shake multiple groups of nutrients, so that the nutrients in the test tube 205 are fully mixed, and the growth environment of the pilose antler mushroom mycelium is ensured, and the plurality of test tubes 205 facilitate the experimenters to perform multiple tests at one time, so as to measure the optimal ratio and required nutrients suitable for the growth of the pilose antler mushroom mycelium.

[0051] Further embodiments

[0052] Please refer to Figures 1 to 6 The genetic improvement experiment platform and method for pilose antler mushroom factory production includes a sliding block 301 slidingly connected inside the spiral groove 114, an end of the sliding block 301 away from the spiral groove 114 is fixedly connected with a driven ring 302 arranged outside the rotating shaft 106, the inside of the driven ring 302 is provided with a circular hole with a size matched with the rotating shaft 106, the outer wall of the driven ring 302 is provided with a groove with a size matched with a rotating block 303, the outer wall of the driven ring 302 is rotatably connected with the rotating block 303, the outside of the rotating block 303 is fixedly connected with a plurality of connecting rods 304, the connecting rods 304 are centrally symmetrically distributed with the center point of the rotating block 303 as the symmetric center, among them, two axially symmetrically arranged connecting rods 304 are internally provided with through holes with sizes matched with a movement track fixing rod 306, an end of the connecting rod 304 away from the rotating block 303 is fixedly connected with a limiting ring 305, the inside of the limiting ring 305 is provided with a sliding groove with a size matched with a fixed block 307, the inside of the limiting ring 305 is provided with a through hole with an elliptical cross section, the inside of the two axially symmetrically arranged connecting rods 304 is provided with the movement track fixing rod 306, the bottom end of the movement track fixing rod 306 is fixedly connected to the placement platform 201, the inside of the limiting ring 305 is rotatably connected with the fixed block 307, the inside of the fixed block 307 is provided with an air pipe 308, the air pipe 308 is connected with a gas output port of an external gas storage device, the bottom end of the air pipe 308 is fixedly connected with a piston 309, and the device further includes a constant temperature room 401 arranged outside the test tube 205, the constant temperature room 401 is arranged at the bottom of the placement platform 201, and the inside of the constant temperature room 401 is provided with a heating device.

[0053] The effects achieved by the embodiment are as follows: compared with the prior art, the device places the pilose antler mushroom mycelium in different gas environments, thereby testing the influence of different types of gas environments on the growth of pilose antler mushroom mycelium, and at the same time, the growth condition of the mycelium in different temperature environments can be tested, so as to obtain the optimal gas environment and temperature suitable for the growth of pilose antler mushroom mycelium.

[0054] Further embodiments

[0055] The improved method for the strain selection experiment platform in the pilose antler mushroom factory production as described above comprises the following steps:

[0056] Step 1: preparation of culture medium:

[0057] Step 1.1: obtain bran, poplar sawdust, bean dregs, yield enhancer, superphosphate, humic acid and distilled water in proportion, and mix them thoroughly;

[0058] Step 1.2: put the mixture obtained in step 1.1 into the test tube 205, start the external motor, make the rotating shaft 106 rotate, and indirectly make the positioning block 109 shake the mixture in the test tube 205, and the preparation of the culture medium is completed;

[0059] Step 2: test of growth required environment:

[0060] Step 2.1: put an equal amount of pilose antler mushroom mycelium into the test tube 205 treated in step 1;

[0061] Step 2.2: inject different proportions and types of mixed gas into different test tubes 205 in step 2.1;

[0062] Step 2.3: place the mycelium treated in step 2.2 in an outdoor environment at different temperatures, and keep the temperature constant, wherein the ambient temperature ranges from 20℃ to 25℃;

[0063] Step 3: observe the growth condition of pilose antler mushroom:

[0064] Step 3.1: observe and record the growth condition of pilose antler mushroom mycelium in different test tubes 205 treated in step 2 within 60 days.

[0065] In step S1.1, 25 parts of bran, 25 parts of poplar sawdust, 4 parts of bean dregs, 4 parts of yield enhancer, 1 part of superphosphate, 1 part of humic acid and 25 parts of distilled water are obtained in proportion.

[0066] The gas injected into the test tube in step S2.2 is selected from two or three of carbon dioxide, nitrogen, methane and oxygen.

[0067] The working process and principle of the overall content of the above embodiment are as follows:

[0068] First, the different proportions of nutrients are respectively put into different test tubes 205, and the test tubes 205 are labeled, and then the test tubes 205 are placed in the positioning ring 202, and the positioning ring 202 fixes the position of the test tubes 205;

[0069] Then the staff starts the external motor, and the rotating shaft 106 connected with the power output shaft of the external motor rotates under the action of the external motor, at this time the spiral groove 114 slidingly connected on the outside of the rotating shaft 106 will move, causing the sliding block 301 arranged in the spiral groove 114 to move downward along the spiral groove 114, and the driven ring 302 fixedly connected with the sliding block 301 will also move;

[0070] Since the rotating block 303 is rotatably connected to the outer wall of the driven ring 302, and the connecting rod 304 is fixedly connected to the outside of the rotating block 303, the rotating block 303 and the connecting rod 304 will move with the driven ring 302, and in the process of moving, the connecting rod 304 will move vertically downward under the action of the movement track fixed rod 306 penetrating through the inside of the connecting rod 304, causing the limiting ring 305 fixedly connected to the other end of the connecting rod 304 to move synchronously, at this time the fixed block 307 arranged in the limiting ring 305, the air pipe 308 arranged in the fixed block 307, and the piston 309 connected with the air pipe 308 will move downward, and the piston 309 will move towards the test tube 205 until it is inserted into the test tube 205;

[0071] With the rotation of the rotating shaft 106, the sliding block 301 drives the driven ring 302 to gradually move to the end of the spiral groove 114, in this process, the piston 309 gradually inserted into the test tube 205 will drive the test tube 205 to move downward synchronously under the action of friction, causing the fixed ring 204 arranged outside the test tube 205 to move downward synchronously through the elastic connecting piece 203 and the positioning ring 202, and the placing platform 201 arranged outside the positioning ring 202;

[0072] The movement of the placing platform 201 will cause the rotating shaft 106 connected thereto to move downward, compressing the telescopic rod 105 at the same time, and the rotating shaft 106 will gradually move towards the rotating piece 104 until it is engaged with the rotating piece 104, in this process, the position of the test tube 205 will also be lowered with the lowering of the placing platform 201, and when the rotating shaft 106 is engaged with the rotating piece 104, the bottom of the test tube 205 will be embedded in the positioning block 109;

[0073] The rotating shaft 106 in the engaged state and the rotating part 104 will rotate synchronously under the action of the external motor, at this time the wedge block 103 fixedly connected with the rotating part 104 will rotate, so that the extrusion part 107 abutting the outer wall of the wedge block 103 will reciprocate under the action of the wedge block 103, and the positioning block 109 connected with the extrusion part 107 through the connecting rod 108 will also move, and during the movement, the positioning block 109 will reciprocate along an elliptical trajectory under the action of the columnar body 110 arranged at the bottom of the connecting rod 108 and the elliptical groove arranged on the limiting block 111;

[0074] At this time, the bottom of the test tube 205 clamped in the positioning block 109 will move synchronously under the action of the positioning block 109, and during the movement, the top of the test tube 205 will also swing because the fixed ring 204 is arranged outside the top of the test tube 205 and the elastic connecting part 203 is arranged outside the fixed ring 204, so that the test tube 205 can be evenly distributed in the test tube 205, avoiding manual shaking of multiple test tubes 205, and the substances in the test tube 205 can be quickly and fully mixed, so that the growth of mycelium will not be affected by uneven distribution of nutrients, thereby achieving the purpose of shaking the nutrients in the test tube 205;

[0075] After the test tube 205 is shaken, the external motor drives the rotating shaft 106 to rotate reversely, the rotating shaft 106 drives the sliding block 301 to move reversely, and other structures also move reversely according to the above steps, so that the piston 309 is separated from the test tube 205;

[0076] Then the staff puts the deer mushroom mycelium into the test tube 205, and then starts the external motor again to make the rotating shaft 106 rotate forward, and after moving according to the above movement process, the piston 309 is clamped into the test tube 205 again, at this time the rotating shaft 106 is driven to rotate;

[0077] Then the staff injects different kinds of gas into different test tubes 205 through the gas pipe 308, so as to simulate the growth of deer mushroom mycelium in different gas environments, and test the stress resistance of deer mushroom mycelium, such as the growth demand of deer mushroom mycelium for carbon dioxide, nitrogen, methane and oxygen, and the adaptability of deer mushroom mycelium to pH, so as to ensure the repeatability and scientificity of the experiment;

[0078] During the test, the thermostat room 401 can also be adjusted to different temperatures to test the growth of deer mushroom mycelium under different temperatures, so as to determine the best temperature suitable for the growth of deer mushroom mycelium.

[0079] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0080] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A platform for genetic improvement of a strain in the industrial production of deer antler fungus, comprising a base plate (101), characterized in that: The bottom plate (101) center position top rotation is connected with the pivot (102), the pivot (102) is fixedly connected with the wedge block (103) away from the bottom plate (101) one end, the wedge block (103) center position top fixedly connected with the rotating part (104), the rotating part (104) top rotation is connected with the telescopic link (105), the telescopic link (105) top is provided with the rotation shaft (106) with the rotating part (104) engagement, the wedge block (103) outside surrounding wedge block (103) is provided with extrusion piece (107), the extrusion piece (107) is with wedge block (103) centre point as the symmetric center center symmetry is arranged, the extrusion piece (107) is rotationally connected with the connecting rod (108) on the side away from wedge block (103), the connecting rod (108) is rotationally connected with the positioning block (109) on the end away from extrusion piece (107), the connecting rod (108) is close to the lower side of the one end of extrusion piece (107) and is provided with the columnar body (110), the columnar body (110) outside is provided with the limit block (111), the limit block (111) top is provided with oval slide groove (112), the limit block (111) is close to the one side of pivot (102) and is provided with the fixed part (113) with one end fixedly connected on the outside of pivot (102), the rotation shaft (106) outside is provided with helical groove (114), the helical groove (114) is with pivot (106) axle center as the symmetric center center symmetry distribution; It also includes the placement platform (201) arranged outside the rotation shaft (106), a plurality of positioning rings (202) are embedded on the placement platform (201), the positioning rings (202) are centrally symmetrically distributed with the center point of the placement platform (201) as the symmetric center, the elastic connecting pieces (203) are slidably connected inside the positioning rings (202), the fixed rings (204) are fixedly connected to the positioning rings (202) on the ends away from the positioning rings (202), the test tubes (205) are clamped inside the fixed rings (204), the stretchers (206) are fixedly connected to the limiting pieces (207) arranged outside the test tubes (205) on the ends away from the test tubes (205), and the limiting pieces (207) are provided with the through grooves with trapezoidal cross sections inside. The experimental platform for genetic improvement of the strain in the factory production of pilose antler mushroom further includes a sliding block (301) slidingly connected inside the spiral groove (114), one end of the sliding block (301) away from the spiral groove (114) being fixedly connected with a driven ring (302) arranged outside the rotating shaft (106), the outer wall of the driven ring (302) being rotatably connected with a rotating block (303), the outer side of the rotating block (303) being fixedly connected with a plurality of connecting rods (304) which are centrally symmetrically distributed with the center point of the rotating block (303) as the center of symmetry; One end of each of the connecting rods (304) away from the rotating block (303) is fixedly connected with a limiting ring (305), an elliptical through hole being formed in the inside of the limiting ring (305), and a moving track fixing rod (306) being penetratingly arranged in the inside of each of the two axisymmetrically arranged connecting rods (304), the bottom end of the moving track fixing rod (306) being fixedly connected on the placement platform (201). The inside of each of the limiting rings (305) is rotatably connected with a fixed block (307), and the inside of each of the fixed blocks (307) is provided with an air pipe (308), and the bottom end of each of the air pipes (308) is fixedly connected with a piston (309).

2. The experimental platform for genetic improvement of the strain in the factory production of Cervi Corni Stabili according to claim 1, characterized in that: One end of the extruding piece (107) away from the wedge block (103) penetrates through the top of the fixing piece (113), and the outside of the extruding piece (107) is provided with a spring, and the columnar body (110) is slidingly connected in the oval-shaped sliding groove (112).

3. The experimental platform for genetic improvement of the strain in the factory production of Cervus elephus L. and mushroom according to claim 1, characterized in that: The experimental platform for genetic improvement of the strain in the factory production of pilose antler mushroom further includes a constant temperature room (401) arranged outside the test tube (205), and the constant temperature room (401) is arranged at the bottom of the placement platform (201).

4. The improvement method of the experimental platform for genetic improvement of the strain in the factory production of pilose antler mushroom according to any one of claims 1-3, characterized in that, The method comprises the following steps: Step 1: preparation of culture medium Step 1.1: obtain bran, poplar sawdust, bean dregs, yield enhancer, superphosphoric acid calcium, humic acid and distilled water in proportion, and mix them thoroughly; Step 1.2: pour the mixture obtained in step 1.1 into the test tube (205), start the external motor, and make the rotating shaft (106) rotate, so as to indirectly make the positioning block (109) shake the mixture in the test tube (205) evenly, and the preparation of the culture medium is completed; Step 2: test of environment required for growth Step 2.1: pour an equal amount of pilose antler mushroom mycelium into the test tube (205) treated in step 1; Step 2.2: inject different proportions and types of mixed gas into different test tubes (205) in step 2.1; Step 2.3: place the mycelium treated in step 2.2 in an outdoor environment with different temperatures, and keep the temperature constant, wherein the temperature of the external environment ranges from 20℃ to 25℃; Step 3: observe the growth of pilose antler mushroom Step 3.1: observe and record the growth of pilose antler mushroom mycelium in different test tubes (205) treated in step 2 within 60 days.

5. The method of claim 4, wherein the genetically modified strain is a genetically modified strain of Lactobacillus plantarum. The proportions in step 1.1 are as follows: bran 20-30 parts, poplar sawdust 20-30 parts, bean dregs 4-5 parts, yield enhancer 4-5 parts, superphosphoric acid calcium 1-2 parts, humic acid 1-2 parts, and distilled water 20-30 parts.

6. The method of improving a bacterial strain genetic improvement test platform according to claim 4, wherein, Step 2.2 Injecting gas into the test tube (205), the gas is selected from two or three of carbon dioxide, nitrogen, methane, and oxygen.

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