Health-care product rich in ergothioneine and preparation method thereof

Abstract

The invention relates to the technical field of health-care products, in particular to a health-care product rich in ergothioneine and a preparation method of the health-care product. The health-care product rich in ergothioneine comprises the following raw materials in parts by weight: 1-5 parts of ergothioneine, 30-50 parts of crude fiber powder, 10-20 parts of fruit freeze-dried powder, 10-30 parts of egg white powder, 5-10 parts of a binder, 1-5 parts of a flavoring agent, 1-3 parts of an antibacterial agent and 1-3 parts of an antioxidant. The raw materials are uniformly mixed and tableted to obtain the health-care product rich in ergothioneine. The health-care product disclosed by the invention has multiple effects, can condition a digestive system, also has the effects of resisting oxidation, resisting aging, beautifying and expelling toxins, and has a positive effect on conditioning daily body functions of people.

Description

Technical Field

[0001] The present invention relates to the technical field of health products, and particularly relates to a health product rich in ergothioneine and a preparation method thereof. Background Art

[0002] Health products are foods with specific health functions or foods aimed at supplementing vitamins and minerals, that is, foods suitable for specific populations to consume, having the function of regulating the body's functions, not aiming at treating diseases, and not causing any acute, subacute or chronic harm to the human body.

[0003] Due to work and life pressure, unhealthy and irregular diet, and disrupted living schedules, etc., the body functions of existing people are often in a sub-healthy state. On the one hand, the long-term sub-healthy state of the body functions not only affects people's physical health, on the other hand, it also affects people's emotions, sleep, memory, etc., seriously affecting people's work and study status as well as the quality of life.

[0004] Therefore, currently, people often adopt certain auxiliary means to regulate their body functions, and health products are one of them. With the development of society, various health products emerge in an endless stream and have numerous effects. However, there are still few reports on health products for regulating the digestive system and detoxifying and beautifying the skin. Therefore, the present invention proposes a health product rich in ergothioneine, which has functions such as promoting digestion, antioxidation, anti-aging, and detoxifying and beautifying the skin, and has a positive effect on regulating people's daily body functions. Summary of the Invention

[0005] The purpose of the present invention is to provide a health product rich in ergothioneine and a preparation method thereof to solve the problem that existing health products are not prominent in functions such as regulating digestive function and detoxifying and beautifying the skin.

[0006] To achieve the above purpose, the present invention adopts the following technical scheme: A health product rich in ergothioneine, comprising the following raw material components in parts by weight: 1 - 5 parts of ergothioneine, 30 - 50 parts of crude fiber powder, 10 - 20 parts of freeze-dried fruit powder, and 10 - 30 parts of egg white powder.

[0007] Further, the ergothioneine is derived from microbial fermentation or mushroom extract; when the source is mushroom extract, the parts by weight of the mushroom extract are converted according to the amount of ergothioneine contained therein, and the parts by weight of other components remain unchanged.

[0008] Further, the preparation of the microbial fermentation includes the following steps:

[0009] S11. Take the ergothioneine fermentation broth, centrifuge and filter it to obtain wet thalli;

[0010] S12. Mix the wet bacterial cells obtained in S11 with absolute ethanol, then perform high-pressure homogenization, centrifuge and filter to obtain a primary extract.

[0011] S13. Subject the obtained primary extract to decolorization and ultrafiltration, take the purified liquid to obtain a secondary extract.

[0012] S14. Concentrate the secondary extract under reduced pressure, crystallize, separate and dry to obtain the ergothioneine produced by microbial fermentation of the present invention.

[0013] Further, in S12, the volume-mass ratio of absolute ethanol to wet bacterial cells is 60-100:1; the pressure of high-pressure homogenization is 60-80 Mpa, and the number of times is 2-4 times; in S13, decolorization includes decolorization with activated carbon, and the cut-off molecular weight of the ultrafiltration membrane is 1000-3000 Da; in S14, crystallization is carried out in a stepwise cooling manner, cooling at 1-2 °C / h to -10-0 °C and holding for 12-24 h.

[0014] Further, the preparation of the mushroom extract includes the following steps:

[0015] S21. Wash, slice, vacuum freeze-dry and crush fresh mushrooms to obtain mushroom powder.

[0016] S22. Mix the mushroom powder in S21 with absolute ethanol, then perform high-pressure homogenization to obtain a suspension.

[0017] S23. Under the conditions of stirring and ultrasound, extract the suspension obtained in S22 to obtain an extract.

[0018] S24. Concentrate and dry the extract to obtain the mushroom extract of the present invention.

[0019] Further, in S21, the mushrooms include Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, Boletus edulis and Hericium erinaceus; the particle size of the mushroom powder is 100-200 mesh; in S22, the volume-mass ratio of absolute ethanol to mushroom powder is 3-10:1; the pressure of high-pressure homogenization is 60-80 Mpa, and the number of times is 2-4 times; in S23, the stirring intensity is 60-120 rpm; the ultrasonic frequency is 20-40 kHz, the power is 300-500 W, and the extraction time is 10-30 min.

[0020] Further, the particle size of the crude fiber powder is 100-200 mesh, and the sources of crude fiber include celery, bracken, spinach and pumpkin; freeze-dry the sources of crude fiber and then crush them to obtain crude fiber powder; the sources of fruits in the freeze-dried fruit powder include oranges, lemons, kiwifruits, strawberries and mangoes; freeze-dry the sources of fruits and then crush them to obtain freeze-dried fruit powder, and the particle size of the freeze-dried fruit powder is 100-200 mesh.

[0021] Furthermore, it also includes 5-10 parts by weight of binder, 1-5 parts by weight of flavoring agent, 1-3 parts by weight of antibacterial agent, and 1-3 parts by weight of antioxidant. The binder includes starch paste; the flavoring agent includes maltol; the antibacterial agent includes curcumin; the antioxidant includes tea polyphenols.

[0022] Preparation method of health care product rich in ergothioneine, comprising the following steps:

[0023] S31. Mix ergothioneine, crude fiber powder, freeze-dried fruit powder, egg white powder, regulator, antibacterial agent, and antioxidant evenly to obtain a uniformly mixed powder raw material;

[0024] S32. Add the binder to the uniformly mixed powder raw material obtained in S31, disperse evenly and then press into tablets to obtain the health care product rich in ergothioneine of the present invention.

[0025] Furthermore, in S31, the mixing is carried out under the protection of nitrogen, the mixing temperature is room temperature, the stirring intensity during mixing is 60-120 rpm, and the mixing time is 30-90 min.

[0026] Beneficial effects of the present invention:

[0027] 1. The present invention can promote intestinal peristalsis and absorption, and has a positive effect on regulating gastrointestinal function;

[0028] 2. The present invention can scavenge active oxides in the body, has antioxidant and anti-aging effects, and has the function of beautifying and anti-aging;

[0029] 3. The present invention has the function of detoxification, can remove impurities and heavy metals in the body, so as to realize the regulation of body functions. Specific embodiments

[0030] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention.

[0031] Example 1

[0032] In this embodiment, ergothioneine is derived from microbial fermentation, and the steps of microbial fermentation extraction are:

[0033] Take the ergothioneine fermentation broth (slant culture of the mycelium of Pleurotus sapidus), centrifuge and filter it to obtain wet mycelium; add absolute ethanol to the obtained wet mycelium, and the volume-mass ratio of absolute ethanol to wet mycelium is 80:1. After stirring evenly, perform high-pressure homogenization using a high-pressure homogenizer. The pressure of high-pressure homogenization is 80 Mpa, and homogenize 3 times; centrifuge and filter the material after high-pressure homogenization to obtain the first extract; decolorize the first extract with activated carbon and perform ultrafiltration with a cut-off molecular weight of 2000 Da, take the purified liquid to obtain the second extract; concentrate the second extract under reduced pressure, then cool it to -10 °C at a rate of 2 °C / h and keep it warm for 24 h, centrifuge and dry it to obtain pure ergothioneine. After detection, the purity of the obtained ergothioneine is 99.61%.

[0034] By weight, take 3 parts of the above-mentioned ergothioneine, 40 parts of celery-derived crude fiber, 10 parts of freeze-dried orange fruit powder, 20 parts of egg white powder, 3 parts of maltol, 1 part of curcumin and 1 part of tea polyphenol. Mix the above raw materials in a mixing kettle. When mixing, introduce nitrogen for protection. The mixing and stirring intensity is 80 rpm, and the mixing time is 30 min. After mixing evenly, continue to add 10 parts of 10% starch slurry to the mixing kettle, maintain the stirring intensity unchanged, and continue to stir and mix for 30 min. After the stirring is completed, tablet press the tablets to obtain the health product rich in ergothioneine of the present invention.

[0035] Example 2

[0036] In this example, ergothioneine is derived from a mushroom extract, and the preparation method of the mushroom extract is as follows:

[0037] Take Pleurotus eryngii, wash it and cut it into thin slices with a thickness of 1-3 mm. Vacuum freeze-dry the thin slices to constant weight, then crush them and pass through a 200-mesh sieve to obtain Pleurotus eryngii powder. Take anhydrous ethanol with a volume-weight ten times that of Pleurotus eryngii powder and perform homogenization in a high-pressure homogenizer. The homogenization pressure is 80 Mpa, and the number of homogenization times is 3 times.

[0038] After homogenization, perform extraction under stirring and ultrasonic conditions, where the stirring intensity is 100 rpm, the ultrasonic frequency is 30 kHz, the intensity is 400 W, and the stirring extraction time is 20 min. After extraction, filter to obtain the extract. Concentrate the extract under reduced pressure at 60 °C, and then vacuum dry it to constant weight at 60 °C to obtain the mushroom extract of the present invention; after detection, the content of ergothioneine in the mushroom extract is 22.37%.

[0039] By weight, take 15 parts of the above-mentioned mushroom extract, 50 parts of coarse fiber from bracken, 15 parts of freeze-dried fruit powder from kiwifruit, 20 parts of egg white powder, 3 parts of maltol, 1 part of curcumin, and 1 part of tea polyphenol. Mix the above raw materials in a mixing kettle. During mixing, nitrogen is introduced for protection. The mixing and stirring intensity is 80 rpm, and the mixing time is 30 min. After mixing evenly, continue to add 10 parts of 10% starch slurry to the mixing kettle, maintain the stirring intensity unchanged, and continue to stir and mix for 30 min. After stirring is completed, press tablets with a tablet press to obtain the health care product rich in ergothioneine of the present invention.

[0040] Example 3

[0041] In this example, the ergothioneine obtained in Example 1 is used.

[0042] By weight, take 5 parts of the above-mentioned ergothioneine, 40 parts of coarse fiber from pumpkin, 20 parts of freeze-dried fruit powder from lemon, 25 parts of egg white powder, 3 parts of maltol, 1 part of curcumin, and 3 parts of tea polyphenol. Mix the above raw materials in a mixing kettle. During mixing, nitrogen is introduced for protection. The mixing and stirring intensity is 80 rpm, and the mixing time is 30 min. After mixing evenly, continue to add 10 parts of 10% starch slurry to the mixing kettle, maintain the stirring intensity unchanged, and continue to stir and mix for 30 min. After stirring is completed, press tablets with a tablet press to obtain the health care product rich in ergothioneine of the present invention.

[0043] Randomly invite 50 people to sign the product informed consent form and taste the products in Examples 1-3. After tasting, the products of the present invention have good flavor and taste.

[0044] Randomly invite 90 people to sign the product informed consent form, divide them into 3 groups on average, and conduct a 30-day usage experience on the products in Examples 1-3 respectively. The data statistics results are as follows in the table:

[0045] Table 1 Data table for product effect evaluation

[0046]

[0047] It can be seen that after using the health care product of the present invention, before and after use, there are obvious improvements in the body functions of the users. During the evaluation process of the health care product of the present invention, the improvement rates of insomnia, anorexia, and constipation all reach more than 75%. It has a good effect on regulating the body functions. In addition, it is found in the actual measurement that the complexion of the tested persons has obvious improvements before and after the test, indicating that the health care product of the present invention also has a good effect on improving the complexion.

[0048] Take the health products obtained in Examples 1-3, grind them into powder, and test their heavy metal removal ability. During the process, prepare a 0.001 mol / L lead nitrate solution. Take three 100 mL portions of the prepared lead nitrate solution and add 10 g of the health product of the present invention prepared in Examples 1-3 to each of them. Stir at 60 rpm for 30 min and then filter. Measure the lead content in the filtrate. The results are as follows:

[0049] Table 2 Removal effect of the product on heavy metals

[0050]

[0051] It can be seen that the health product of the present invention has a good heavy metal removal effect, can remove toxins from the body, and has a positive effect on regulating the body function.

[0052] The present invention is not limited to the above best implementation manner. Any person can obtain other various forms of products under the inspiration of the present invention. However, no matter what changes are made in its shape or structure, as long as it has the same or similar technical solutions as this application, it falls within the protection scope of the present invention.

Claims

1. A health product rich in ergothioneine, characterized in that, It comprises the following raw materials in parts by weight: 1-5 parts of ergothioneine, 30-50 parts of crude fiber powder, 10-20 parts of freeze-dried fruit powder, and 10-30 parts of egg white powder.

2. The health product rich in ergothioneine according to claim 1, characterized in that The ergothioneine is derived from microbial fermentation or mushroom extract; when the source is mushroom extract, the parts by weight of the mushroom extract are converted based on the amount of ergothioneine contained therein, and the parts by weight of other components remain unchanged.

3. A health product rich in ergothioneine according to claim 2, characterized in that, The preparation of the microbial fermentation comprises the following steps: S11. Take the ergothioneine fermentation broth, centrifuge and filter to obtain wet thalli. S12. Mix the wet thalli obtained in S11 with absolute ethanol, then perform high-pressure homogenization, centrifuge and filter to obtain a primary extract. S13. Decolorize and ultrafilter the obtained primary extract, take the purified liquid to obtain a secondary extract. S14. Concentrate the secondary extract under reduced pressure, crystallize, separate and dry to obtain the microbial fermentation ergothioneine of the present invention.

4. The health product rich in ergothioneine according to claim 3, wherein: In S12, the volume-mass ratio of absolute ethanol to wet thalli is 60-100:1; the pressure of high-pressure homogenization is 60-80 Mpa, and the number of times is 2-4 times; in S13, decolorization includes activated carbon decolorization, and the cut-off molecular weight of the ultrafiltration membrane is 1000-3000 Da; in S14, crystallization is carried out in a stepwise cooling manner, cooling at 1-2 °C / h to -10-0 °C, and keeping warm for 12-24 h.

5. A health product rich in ergothioneine according to claim 2, characterized in that, The preparation of the mushroom extract comprises the following steps: S21. Wash, slice, vacuum freeze-dry and crush fresh mushrooms to obtain mushroom powder. S22. Mix the mushroom powder obtained in S21 with absolute ethanol, then perform high-pressure homogenization to obtain a suspension. S23. Under the conditions of stirring and ultrasonic wave, extract the suspension obtained in S22 to obtain an extract. S24. Concentrate and dry the extract to obtain the mushroom extract of the present invention.

6. The health care product rich in ergothioneine according to claim 4, wherein: In S21, the mushrooms include Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, Boletus edulis and Hericium erinaceus; the particle size of the mushroom powder is 100-200 mesh; in S22, the volume-mass ratio of absolute ethanol to mushroom powder is 3-10:1; the pressure of high-pressure homogenization is 60-80 Mpa, and the number of times is 2-4 times; in S23, the stirring intensity is 60-120 rpm; the ultrasonic frequency is 20-40 kHz, the power is 300-500 W, and the extraction time is 10-30 min.

7. A health product rich in ergothioneine according to claim 1, characterized in that: The particle size of the crude fiber powder is 100-200 mesh, and the sources of crude fiber include celery, bracken, spinach and pumpkin; the crude fiber source is freeze-dried and then crushed to obtain the crude fiber powder; the fruit sources in the freeze-dried fruit powder include oranges, lemons, kiwifruits, strawberries and mangoes; the fruit source is freeze-dried and then crushed to obtain the freeze-dried fruit powder, and the particle size of the freeze-dried fruit powder is 100-200 mesh.

8. A health product rich in ergothioneine according to claim 1, characterized in that: It also includes 5-10 parts by weight of binder, 1-5 parts by weight of flavoring agent, 1-3 parts by weight of antibacterial agent and 1-3 parts by weight of antioxidant. The binder includes starch paste; the flavoring agent includes maltol; the antibacterial agent includes curcumin; the antioxidant includes tea polyphenols.

9. The preparation method of the ergothioneine-rich health product according to any one of claims 1-8, characterized in that, It comprises the following steps: S31. Mix ergothioneine, crude fiber powder, freeze-dried fruit powder, egg white powder, regulator, antibacterial agent and antioxidant evenly to obtain a uniformly mixed powder raw material; S32. Add the binder to the uniformly mixed powder raw material obtained in S31, disperse it evenly and then press tablets to obtain the health product rich in ergothioneine of the present invention.

10. The preparation method of the ergothioneine-rich health product according to claim 9, characterized in that: In the said S31, the mixing is carried out under the protection of nitrogen, the mixing temperature is room temperature, the stirring intensity during mixing is 60 - 120 rpm, and the mixing time is 30 - 90 min.

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