Application of sargassum pallidum polypeptide in preparation of whitening cosmetics

Abstract

The invention belongs to the technical field of marine organisms, and discloses application of sargassum pallidum polypeptide in preparation of whitening cosmetics. The sargassum pallidum polypeptide is derived from sargassum pallidum and is white-like powder, the peptide content is larger than or equal to 99% (dry basis), the molecular weight is smaller than or equal to 1000 Da, the weight loss on drying is smaller than or equal to 5.0%, the ash content is smaller than or equal to 5.0%, and the sargassum pallidum polypeptide meets the safety limit standards of heavy metal, microorganisms and the like. The sargassum pallidum polypeptide reduces melanin synthesis by inhibiting the activity of tyrosinase, and the half inhibitory mass concentration of the sargassum pallidum polypeptide to tyrosinase is 3.28 mg / mL through determination by a dopa staining method, so that the sargassum pallidum polypeptide shows a clear whitening effect. The high-purity and low-molecular-weight polypeptide component provided by the invention has safety and stability, overcomes the defects of high sensitization, poor transdermal absorption and the like of the traditional whitening component, and provides an innovative solution for efficient utilization of marine resources in the field of cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of marine biotechnology, and particularly to the application of Sargassum pallidum polypeptides in the preparation of whitening cosmetics. Background Art

[0002] In the field of cosmetics, the pursuit of whitening efficacy has always been the focus of consumer attention. Excessive deposition of skin melanin often leads to problems such as uneven skin tone and dullness, and the core regulatory factor in this process is the activity of tyrosinase. Although there are various types of whitening ingredients on the market currently, they generally face dual challenges of safety and stability. For example, some chemically synthesized ingredients have potential sensitization risks or long-term use safety controversies, while some natural extracts are prone to being affected by the environment due to insufficient stability, resulting in the difficulty of lasting efficacy. In addition, many tyrosinase inhibitors in the prior art have problems such as low transdermal absorption efficiency and unsatisfactory bioavailability due to their large molecular weights or complex structures, which limit their actual application effects.

[0003] In recent years, marine bioactive ingredients have gradually become a research hotspot due to their unique structural and functional characteristics. Sargassum pallidum, a marine algal resource widely distributed along the coast of China, is rich in proteins and bioactive substances, but its high-value application has not been fully developed. The active ingredients extracted from Sargassum pallidum by traditional processes often have problems such as insufficient purity and uneven molecular weight distribution, resulting in poor compatibility and unstable efficacy in cosmetic formulations, and may even cause irritating reactions. Therefore, developing a high-purity, low-molecular-weight active polypeptide ingredient derived from Sargassum pallidum, which takes into account safety, stability, and high-efficiency whitening efficacy, has become a technical problem that urgently needs to be broken through in this field.

[0004] In view of the above technical bottlenecks, the present invention proposes an application solution of using Sargassum pallidum polypeptides as a new type of whitening active ingredient. By means of directional enzymatic hydrolysis and fractional purification techniques, a polypeptide component with a high purity and a molecular weight ≤ 1000 Da is prepared from Sargassum pallidum. Combining its specific mechanism of inhibiting tyrosinase activity, it provides an innovative solution for the cosmetics industry that combines the sustainability of marine resources, the safety of use, and the reliability of efficacy, and is expected to promote the technological upgrading and industrial transformation of natural whitening ingredients. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide the application of Sargassum pallidum polypeptides in the preparation of whitening cosmetics in view of the deficiencies of the prior art.

[0006] In order to achieve the object of the present invention, the following technical means are specifically adopted:

[0007] The application of Sargassum pallidum polypeptides in the preparation of whitening cosmetics.

[0008] Sargassum fusiforme polypeptide exerts a whitening effect by inhibiting tyrosinase.

[0009] The Sargassum fusiforme polypeptide described in the present invention is a light - colored powder. The particle size standard is that 100% can pass through an 80 - mesh sieve. The peptide content (dry basis) ≥ 99%, the molecular weight ≤ 1000 Da, the loss on drying ≤ 5.0%, the ash content ≤ 5.0%, no pesticide residues can be detected, the total heavy metal content ≤ 10 ppm, the arsenic content ≤ 10 ppm, the lead content ≤ 10 ppm, the total number of colonies ≤ 1000 cfu / g, the number of yeasts and molds ≤ 100 cfu / g, no Escherichia coli can be detected, no Salmonella can be detected, and no Staphylococcus can be detected.

[0010] Beneficial effects

[0011] The beneficial effects of the present invention are as follows:

[0012] 1. The Sargassum fusiforme polypeptide described in the present invention has high purity, which is not only conducive to precise formulation and stable application in whitening cosmetic formulations, but also can minimize potential risks such as allergies and irritations caused by the presence of impurities, ensuring the safety and reliability of product use.

[0013] 2. Verified by tyrosinase inhibition experiments, the Sargassum fusiforme polypeptide exhibits good tyrosinase inhibitory activity. Tyrosinase plays a central role in the melanin biosynthesis pathway, and the effective inhibition of its activity can accurately and significantly reduce the amount of melanin synthesis, fundamentally laying a solid foundation for achieving skin whitening efficacy. Description of the drawings

[0014] Figure 1 is the inhibition curve of kojic acid on tyrosinase;

[0015] Figure 2 is the inhibition curve of Sargassum fusiforme polypeptide on tyrosinase. Detailed implementation manners

[0016] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and in no way restricts the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0017] Description of the Sargassum fusiforme polypeptide used in the present invention: The manufacturer is Xi'an Mugo Biotechnology Co., Ltd., the product batch number is MG-2024081502, the appearance is off-white powder, the particle size standard is that 100% can pass through an 80-mesh sieve, the peptide content (dry basis) is 99.07%, the molecular weight ≤ 1000 Da, the loss on drying is 0.79%, the ash content is 1.42%, no pesticide residues are detected, the total heavy metals ≤ 10 ppm, the arsenic content ≤ 10 ppm, the lead content ≤ 10 ppm, the total number of colonies ≤ 1000 cfu / g, the number of yeasts and molds ≤ 100 cfu / g, no Escherichia coli is detected, no Salmonella is detected, and no Staphylococcus is detected.

[0018] Example 1

[0019] Experiment on the inhibitory effect of Sargassum fusiforme polypeptide on tyrosinase:

[0020] The dopa staining method was used to determine the effect of the test sample on tyrosinase activity.

[0021] The total volume of the reaction system was 200 μL. 50 μL of PBS phosphate buffer at pH 6.8, 50 μL of tyrosinase solution at 120 U / mL, and 50 μL of samples or reference substances at different concentrations were added in sequence. After preheating at 37 °C for 10 min, 50 μL of L-dopa (0.4 mg / mL, prepared with PBS phosphate buffer at pH 6.8) was added and reacted for 10 min. The absorbance was measured at 475 nm with an enzyme-labeled instrument, and the tyrosinase inhibition rate was calculated. In addition, a negative control group, a background group, and a blank group were set up. The details of each reaction system are shown in Table 1 below. The IC 50 value was calculated through the relationship between the enzyme activity inhibition rate and the concentration curve.

[0022] Table 1 Tyrosinase inhibition reaction systems for different groups (unit: μL)

[0023] Group Control group Background group Sample group Blank group PBS 100 150 50 100 Sample / Kojic acid - - 50 50 Tyrosinase 50 - 50 - L-DOPA 50 50 50 50

[0024] The formula for calculating the tyrosinase inhibition rate is: Inhibition rate (%) = [1 - (A 样品 - A 空白 ) / (A 对照 - A 背景 )] × 100%

[0025] Where: A 对照 is the reaction absorbance value without inhibitor; A 背景 is the reaction absorbance value without inhibitor and tyrosinase; A 样品 is the reaction absorbance value of the sample or positive drug; A 空白 is the reaction absorbance value without tyrosinase.

[0026] Experimental results:

[0027] Table 2 Effects of Sargassum pallidum polypeptides and kojic acid on tyrosinase activity

[0028] Sample <![CDATA[Half inhibitory mass concentration (IC 50 ) / (mg / mL)]]> Sargassum polypeptide 3.28 Kojic acid 0.027

[0029] The above results indicate that Sargassum pallidum polypeptides can inhibit tyrosinase activity.

[0030] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. Use of a polypeptide from Sargassum pallidum in the preparation of a whitening cosmetic, characterized in that, The quality indicators of the Sargassum pallidum polypeptide include: peptide content (on dry basis) ≥ 99%, molecular weight ≤ 1000 Da, loss on drying ≤ 5.0%, ash content ≤ 5.0%, total heavy metals ≤ 10 ppm, and no pesticide residues, Escherichia coli, Salmonella and Staphylococcus are detected; the application achieves the whitening effect by inhibiting the activity of tyrosinase to reduce melanin synthesis.

2. The application according to claim 1, characterized in that, The half inhibitory concentration (IC50) of the Sargassum pallidum polypeptide against tyrosinase is 3.0 - 3.5 mg / mL.

3. The application according to claim 1, characterized in that, The particle size standard of the Sargassum pallidum polypeptide is that 100% passes through a 80-mesh sieve, and the appearance is off-white powder.

4. The application according to claim 1, characterized in that, The total number of colonies of the Sargassum pallidum polypeptide ≤ 1000 cfu / g, and the number of yeasts and molds ≤ 100 cfu / g.

5. A whitening cosmetic, characterized in that, Comprising the Sargassum pallidum polypeptide according to any one of claims 1 - 4 as an active ingredient, and the addition amount of the Sargassum pallidum polypeptide in the cosmetic is 0.1% - 5% (mass percentage).

6. The whitening cosmetic according to claim 5, wherein The dosage form of the cosmetic is lotion, cream or essence.

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