Plant essence mask containing extracellular vesicles and having long-acting anti-aging effect

Abstract

The invention discloses a plant essence mask containing extracellular vesicles and having a long-acting anti-aging effect, and belongs to the field of daily necessities. The product takes an extracellular vesicle extract prepared from centella asiatica as a functional component, and is compounded with a specific moisturizing component, an anti-aging component and an antioxidant component, so that the ideal anti-aging effect and deep moisturizing effect of the product can be effectively guaranteed, and meanwhile, even if the product is stored and treated for a long time or at a relatively high temperature, the anti-aging effect of the product can be effectively improved. And the product can still maintain relatively high performance stability.

Description

Technical Field

[0001] The present invention relates to the field of daily necessities, and particularly to a plant essence mask containing extracellular vesicles and having a long-lasting anti-aging effect. Background Art

[0002] The exosome nanovesicles extracted from plant raw materials by means of high-pressure extraction technology belong to a highly biocompatible active ingredient containing various active substances such as lipids and proteins. Compared with traditional plant extracts, they have better human tissue penetration efficiency. Especially when acting on the skin, they can quickly penetrate into the skin surface and enter the deep layer. And through research, these substances also have various activities including immune regulation, microbiota regulation, antioxidant and anti-aging, etc. Theoretically, they have very excellent skin care effects when applied to skin care products or maintenance products.

[0003] However, due to the differences in plant raw materials and preparation processes, the composition of plant extracellular vesicles varies greatly, and it is difficult to estimate the actual biological activity and biological activity stability of the materials. Especially when applied to mask products with a high water content, a large number of moisturizing ingredients and generally stored at room temperature, the influence of plant extracellular vesicles by other ingredients and external factors increases, and they may not be able to maintain long-term effectiveness, and the activity stability is poor. Summary of the Invention

[0004] Based on the defects existing in the prior art, the purpose of the present invention is to provide a plant essence mask with a long-lasting anti-aging effect. The product uses the extracellular vesicle extract prepared from Centella asiatica as the active ingredient, and at the same time is compounded with specific moisturizing ingredients, anti-aging ingredients and antioxidant ingredients, which can effectively ensure that the product has an ideal anti-aging effect and deep moisturizing effect. At the same time, even after long-term storage or storage at a higher temperature, the product can still maintain high performance stability.

[0005] In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A plant essence mask, comprising a mask cloth and a mask liquid;

[0007] The mask liquid comprises the following components in parts by weight:

[0008] Centella asiatica extracellular vesicle extract 0.05 - 3 parts, Chinese yam extract 0.05 - 3 parts, walnut extract 0.05 - 1 part, betaine 0.5 - 2 parts, sunflower seed extract 0.01 - 4 parts, and dipotassium glycyrrhizinate 0.05 - 0.2 part.

[0009] The plant nanovesicles secreted by Centella asiatica contain rich skin-care active lipids and protein active substances, and they have high biocompatibility with human skin cell tissues. When used as active ingredients, they can quickly penetrate into the deep layer of the skin, resulting in a fast skin-care effect. However, compared with traditional plant extracts, they have fewer protective components and relatively single ingredients. Therefore, when applied to some skin-care products with more complex ingredients, especially those with high biological activity and prone to introducing microorganisms in liquid / gel phases, this substance is easily affected by external factors and undergoes rapid activity attenuation or even inactivation in the short term, making it difficult to achieve good stability. Therefore, in the technical solution of the present invention, in order to ensure that the extracellular vesicle components of Centella asiatica can exert short-term and long-term skin-care and stability effects when applied to products, especially mask products, specific moisturizing components, anti-aging components, and antioxidant components are compounded. Among them, Dioscorea opposita extract contains diosgenin with high antioxidant and anti-aging activities, as well as some proteins and mucilage with moisturizing effects. When the mucilage is introduced into the mask liquid components, it can be loaded on the surface of the Centella asiatica extracellular vesicle extract with lipid affinity. On the one hand, it acts as a protective film to prevent microorganisms or other mask liquid components from affecting its biological activity. On the other hand, it can also improve the mixing uniformity of each component and prevent precipitation of the mask liquid after long-term storage. The walnut extract contains rich sterol substances and flavonoid substances, which can not only effectively improve the moisture content of the skin, but also promote blood circulation in the skin and promote cell renewal to repair damage. At the same time, as a transport carrier, the Centella asiatica extracellular vesicle extract can quickly carry the active ingredients of these two plant extracts to the deep layer of the skin through high permeability to achieve a fast skin-care effect. Betaine has strong moisturizing properties when applied to skin-care products, can form a moisture barrier on the skin surface, and at the same time, this component has antioxidant properties and can play a good antioxidant role when applied to the skin. During the storage of the product, it can also play a certain stabilizing role, eliminate free radicals generated in the product, and thus inhibit the influence of free radicals on the Centella asiatica extracellular vesicle extract. Compared with sunflower oil, a natural antioxidant / moisturizing vegetable oil, in the technical solution of the present invention, sunflower seed extract is used instead to ensure the oil / water balance of the mask liquid. This substance also has certain moisturizing and antioxidant effects, and in mask liquid products with a higher water content, sunflower seed extract has better antioxidant and stability effects.In addition, a certain amount of dipotassium glycyrrhizinate needs to be introduced into the facial mask liquid of the technical solution of the present invention to enhance the compatibility effect of each component. It can improve the skin penetration effect of fat-soluble substances, enhance the skin care ability of each active ingredient, and at the same time, together with betaine and sunflower seed extract, play an antioxidant role in the product, and enhance the biological activity stability of the centella asiatica extracellular vesicle extract. The facial mask liquid not only has a fast skin care rate and good skin care efficacy, but also remains effective even after long-term storage or placement at a relatively high temperature, and the storage limitation is significantly reduced.

[0010] Preferably, the facial mask liquid further comprises 5-10 parts of a solvent skin conditioner;

[0011] More preferably, the skin conditioner comprises at least one of sodium hyaluronate, allantoin, butanediol, glycerol, 1,2-hexanediol, and 1,2-pentanediol;

[0012] More preferably, the facial mask liquid comprises 0.01-0.5 part of sodium hyaluronate, 0.01-0.5 part of allantoin, 1-5 parts of butanediol, 1-5 parts of glycerol, 0.1-1 part of 1,2-hexanediol, and 0.1-1 part of 1,2-pentanediol.

[0013] After introducing the special vesicle component, moisturizing component, antioxidant component, and anti-aging component of the present invention, in order to further improve the instant care effect of the facial mask product, a specific content of skin conditioner is added to the product components. These components can have a synergistic effect with the vesicle component, etc., and based on the transportation effect of the vesicle component, accelerate the penetration and skin care rate, so that the product can achieve good effects after short-term use.

[0014] Preferably, the facial mask liquid further comprises 0.1-3 parts of a plasticizer;

[0015] More preferably, the plasticizer comprises at least one of gelatin, xanthan gum, hydroxyethyl cellulose, ammonium polyacryloyldimethyltaurate, and sodium carboxymethylcellulose;

[0016] More preferably, the facial mask liquid comprises 0.01-0.5 part of gelatin, 0.01-0.5 part of xanthan gum, 0.01-0.5 part of hydroxyethyl cellulose, 0.01-0.5 part of ammonium polyacryloyldimethyltaurate, and 0.01-2 parts of sodium carboxymethylcellulose.

[0017] As described above, the introduction of yam extract can improve the stability and biological activity of the centella asiatica extracellular vesicle extract in the product, and contribute to maintaining the stability of the product component properties. In order to further improve the dosage form stability of the facial mask liquid of the product during storage and use, the present invention also adds a certain amount of plasticizer for compounding with the yam extract.

[0018] Preferably, the facial mask liquid further contains 0.1 to 3 parts of functional additives;

[0019] More preferably, the functional additives include but are not limited to at least one of preservatives, pH stabilizers, and co-solvents.

[0020] Even more preferably, the preservative can be 1,2 - hexanediol or 1,2 - pentanediol as described above in terms of volume, or other commonly used preservatives in cosmetic and skin care products can also be used. The pH stabilizers include at least one of acetic acid, citric acid, sodium bicarbonate, and sodium citrate. The co - solvents include at least one of polydimethylsiloxane, caprylic / capric triglyceride, polymethylsilsesquioxane, cetearyl glucoside, and hydrogenated lecithin.

[0021] On the premise of not affecting the effectiveness of the key components of the present invention, those skilled in the art can introduce some common functional additives in the facial mask liquid according to common knowledge and actual needs in the product. For example, in order to ensure that the product is not easily spoiled or deteriorated, a controlled amount of preservatives is added to the product; in order to improve the solubility of the organic components in the product, an appropriate amount of organic solvents is added to disperse them evenly in the product.

[0022] Preferably, the centella asiatica extracellular vesicle extract can be a commercially available product or can be prepared by the following process:

[0023] After cleaning and drying centella asiatica, it is subjected to cell wall breaking and centrifugation treatment. The obtained filtrate is filtered using a capsule filter. The secondary filtrate containing vesicles is then purified and concentrated in sequence to obtain a crude liquid;

[0024] The residue after the capsule filter filtration treatment is mixed with cellulase, then dispersed in water, and the obtained mixture is heated to 30 - 50 °C and treated for 1.5 - 2.5 h, followed by filtration. The obtained enzymolysis liquid and the crude liquid are mixed according to a mass ratio of (0.5:9.5) - (1.5:8.5) to obtain the centella asiatica extracellular vesicle extract.

[0025] When preparing the Centella asiatica extracellular vesicle extract using the above process, not only can the extracellular vesicles in Centella asiatica be effectively and completely extracted, but also the residue after extraction can be thermally enzymolyzed with cellulase to fully refine and extract the bioactive components in the residue. After mixing with the vesicles, based on compatibility, it can rapidly deliver these active components to the deep layer of the skin in the form of a carrier to achieve skin care functions. In particular, active components such as asiaticoside and asiatic acid have a significantly increased effective utilization rate after enzymolysis. However, the compounding ratio of the vesicle material and the enzymolysis solution after extraction needs to be controlled within the above range. If there is too much vesicle material and the content of active components in the enzymolysis solution is small, the skin care effect of this component itself will be reduced. If the addition amount of the enzymolysis solution is too much, the relative content of the carrier for the residue and other active components of the facial mask liquid is small, and the penetration effect of the product on the skin will be reduced.

[0026] Preferably, the rate during the breaking-wall centrifugation treatment is 4000 - 5000g, and the time is 30 - 60 min.

[0027] Preferably, the pore diameter of the filter membrane of the capsule filter is 0.3 - 0.45 μm.

[0028] Preferably, the purification is carried out using a chromatography column.

[0029] Preferably, when the residue and cellulase are dispersed in water, the mass ratio of the residue, cellulase, and water is 1:(0.02 - 0.03):(10 - 20).

[0030] Preferably, the sunflower seed extract can be a commercially available product or can be obtained by, but not limited to, the following preparation method:

[0031] After shelling, washing, drying, and crushing sunflower seeds, the obtained powder is placed in an extraction solution for ultrasonic extraction treatment, followed by solid-liquid separation. The obtained liquid phase is then centrifuged and dried to obtain the sunflower seed extract.

[0032] More preferably, the extraction solution includes water and ethanol, and the mass ratio of water to ethanol is (9:1) - (7:3).

[0033] As described above, the sunflower seed extract contains various oil-soluble and water-soluble substances such as oils, proteins, and biological polysaccharides. However, due to the relatively high content of oil-soluble substances in seed plants, these substances have relatively low stability and are easily affected by external factors to undergo chemical reactions, thereby affecting the activity stability of the centella asiatica extracellular vesicle extract. Therefore, in the solution of the present invention, the sunflower seed extract is used instead of the common sunflower seed vegetable oil as a functional component, and at the same time, the above-mentioned preferred extraction liquid ratio is used for extraction during preparation, which can effectively ensure that the obtained product not only retains the unique antioxidant and anti-aging components in sunflower seeds, but also has higher overall chemical stability and is more conducive to the long-term activity of the centella asiatica extracellular vesicle extract.

[0034] Further preferably, based on 100 parts of the facial mask liquid, it comprises the following components in parts by weight:

[0035] 0.6 - 0.8 parts of centella asiatica extracellular vesicle extract, 0.6 - 0.8 parts of yam extract, 0.6 - 0.8 parts of walnut extract, 1.5 - 2 parts of betaine, 3 - 4 parts of sunflower seed extract, and 0.05 - 0.1 parts of dipotassium glycyrrhizinate.

[0036] As a key functional component, when the parts by weight of the above components are preferably within the above range, the product can achieve better immediate and long-term anti-aging and moisturizing effects.

[0037] Further preferably, based on 100 parts of the facial mask liquid, it comprises the following components in parts by weight:

[0038] 0.5 - 3 parts of centella asiatica extracellular vesicle extract, 0.5 - 3 parts of yam extract, 0.05 - 1 part of walnut extract, 0.5 - 2 parts of betaine, 0.01 - 4 parts of sunflower seed extract, 0.05 - 0.2 parts of dipotassium glycyrrhizinate, 0.01 - 0.5 parts of sodium hyaluronate, 0.01 - 0.5 parts of allantoin, 1 - 5 parts of butanediol, 1 - 5 parts of glycerol, 0.01 - 0.5 parts of gelatin, 0.01 - 0.5 parts of xanthan gum, 0.01 - 0.5 parts of hydroxyethyl cellulose, 0.01 - 0.5 parts of ammonium polyacryloyldimethyltaurate, 0.01 - 2 parts of sodium carboxymethylcellulose, 0.1 - 1 part of 1,2 - hexanediol, 0.1 - 1 part of 1,2 - pentanediol, 0.1 - 0.3 parts of polydimethylsiloxane, 0.1 - 0.3 parts of hydrogenated lecithin, and the balance in parts by weight of water.

[0039] Further preferably, the preparation method of the facial mask liquid comprises the following steps:

[0040] Mix the components, then heat to 40 - 60 °C and stir for 2 - 3 h, cool down and let stand to obtain the facial mask liquid.

[0041] It should be noted that the mask cloth and the mask liquid in the mask of the present invention can be combined during use (that is, the mask cloth and the mask liquid are separated during packaging and the mask cloth is fully wetted with the mask liquid during use), or they can be mixed before use. Both methods will not affect the storage and use effect of the product.

[0042] The beneficial effect of the present invention is that the present invention provides a plant essence mask with long-term anti-aging effect. The product uses the extracellular vesicle extract prepared from centella asiatica as the active ingredient, and at the same time is compounded with specific moisturizing ingredients, anti-aging ingredients and antioxidant ingredients, which can effectively ensure that the product has an ideal anti-aging effect and deep moisturizing effect. At the same time, even after long-term storage or storage at a higher temperature, the product can still maintain high performance stability. Detailed implementation mode

[0043] In order to better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific examples and comparative examples. The purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention. The experimental reagents and instruments involved in the implementation of the present invention are all common ordinary reagents and instruments unless otherwise specified. Among them, the yam extract is a product produced by Youran Biotechnology Co., Ltd., the walnut extract is a product produced by Xi'an Jincuifang Plant Technology Development Co., Ltd., the centella asiatica extract is a product produced by Guangzhou Aolaina Biotechnology Co., Ltd., the aloe extract is a product produced by Hunan Langlin Biology, the polygonum cuspidatum extract is a product produced by Xi'an Quanao Biology, and the sunflower seed oil is a product produced by Hunan Yamei Company.

[0044] Examples 1-9

[0045] In the examples of the plant essence mask with long-term anti-aging effect of the present invention, the component composition and ratio of the mask liquid of each product are shown in Table 1.

[0046] The preparation method of the mask liquid is: mix each component, then heat up to 50°C and stir for 3 hours, and cool and stand to obtain the mask liquid.

[0047] Among them, the centella asiatica extracellular vesicle extract 1 is a self-made product, and the preparation method includes the following steps:

[0048] The fresh asiatic pennywort was washed, dried, and then added with water (water-to-material ratio of 5:1) and subjected to wall-breaking centrifugation at 4500g for 60 min using a wall-breaking machine. The obtained filtrate was filtered using a GVS capsule filter (membrane pore size 0.45 μm). The obtained secondary filtrate containing vesicles was then subjected to column purification (Capto C 700, mobile phase was pure water) and natural static concentration at 40°C for 12 h to obtain a crude liquid;

[0049] The residue after filtration by the capsule filter was mixed with cellulase, then dispersed in water, and the obtained mixture (residue, cellulase, water mass ratio = 1:0.025:10) was heated to 40°C and treated for 2 h, followed by filtration. The obtained enzymolysis liquid was mixed with the crude liquid at a mass ratio of 1:9 to obtain the asiatic pennywort extracellular vesicle extract.

[0050] Asiatic pennywort extracellular vesicle extract 2 was a self-made product. The difference from asiatic pennywort extracellular vesicle extract 1 was only that the obtained crude liquid was the asiatic pennywort extracellular vesicle extract, and no enzymolysis liquid was prepared and added.

[0051] Asiatic pennywort extracellular vesicle extract 3 was a self-made product. The difference from asiatic pennywort extracellular vesicle extract 1 was only that the obtained enzymolysis liquid was mixed with the crude liquid at a mass ratio of 3:7.

[0052] Sunflower seed extract 1 was a self-made product. The preparation method included the following steps:

[0053] After the sunflower seeds were shelled, washed, dried, and crushed, the obtained powder was placed into the extraction liquid at a material-to-liquid ratio of 1:10 and subjected to ultrasonic extraction for 12 h. After solid-liquid separation, the obtained liquid phase was further centrifuged and dried to obtain the sunflower seed extract. The extraction liquid was a mixed liquid prepared by compounding water and ethanol at a mass ratio of 9:1.

[0054] Sunflower seed extract 2 was a self-made product. The difference from sunflower seed extract 1 was only that the extraction liquid was a mixed liquid prepared by compounding water and ethanol at a mass ratio of 7:3.

[0055] Sunflower seed extract 3 was a self-made product. The difference from sunflower seed extract 1 was only that the extraction liquid was a mixed liquid prepared by compounding water and ethanol at a mass ratio of 1:1.

[0056] Sunflower seed extract 4 was a self-made product. The difference from sunflower seed extract 1 was only that the extraction liquid was pure water.

[0057] Comparative examples 1 - 9

[0058] A facial mask, the difference from the examples was only that the component compositions and ratios of each facial mask liquid were different, as shown in Table 2.

[0059] Unless otherwise specified, the component raw materials used in the embodiments and comparative examples of the present invention are all commercially available raw materials, and the component raw materials used in each parallel experiment are of the same kind.

[0060] Table 1

[0061]

[0062]

[0063] Table 2

[0064]

[0065]

[0066] Effect Example 1

[0067] In order to explore the effects of the products of the present invention, first, the products of each embodiment and comparative example were subjected to a safety test experiment according to SNT2329-2009. The results showed that the products of each embodiment and comparative example were classified as non-irritating, and the products could be used for skin care.

[0068] Subsequently, the products were placed in environments of 0 °C, 25 °C, and 48 °C for 30 days respectively, and it was observed whether there were precipitation and turbidity phenomena in the products (the products stored at 0 °C in the refrigerator were first restored to 25 °C and then observed). After testing, no abnormal phenomena occurred in each product, indicating that the product dosage form had good stability.

[0069] Based on the above test results, biological experiment tests were carried out on the products:

[0070] Elastase inhibition rate. The test was based on the LC-WI-HZ-176 Elastase Inhibition Test Operating Instruction 1.0. The test environment was 18-28 °C and 45-75% relative humidity. The incubation temperature of elastase was 25 °C and the incubation time was 15 min. When testing, the test wavelength of the microplate reader was 410 nm, and the test data was the inhibition rate OD value; when testing, the initial inhibition rate of the product was pre-tested. Subsequently, the products of each embodiment and comparative example were placed in a packaging bag for encapsulation, filled with nitrogen, and stored at 40 °C for 20 days (if bulging occurred, air extraction and exhaust treatment were carried out), and then the same test was carried out to confirm the secondary inhibition rate of the product after storage treatment. The test results are shown in Table 3.

[0071] Table 3

[0072]

[0073]

[0074] It can be clearly seen from Table 3 that since elastase has the effect of controlling the elasticity of human skin surface tissue, controlling it can achieve better anti-skin aging and wrinkle phenomena. Therefore, the products of each example have good anti-skin aging effects. Especially after long-term storage at 40°C, although the inhibition rate of the products shows a certain decline, it all remains above 10%, and the effect is excellent. According to the test results of the product of Comparative Example 1 and the products of the examples, the extracellular vesicles extract of Centella asiatica has a certain effect of inhibiting elastase. When this ingredient is not introduced, the initial anti-aging performance of the product is relatively low. And even when introducing the Centella asiatica extract with certain similar ingredients, as shown in Comparative Example 2, it is still inferior to the corresponding product of Example 1. The main reason is that the extracellular vesicles extract of Centella asiatica has higher biological active ingredients, fewer impurities, and faster active effects compared to the Centella asiatica extract prepared by the conventional extraction process. According to the comparison of the test results of the products of Example 1 and Examples 5-6, it can be seen that when preparing the extracellular vesicles extract of Centella asiatica, the ratio of the crude liquid containing vesicles to the enzymatic hydrolysis solution has an impact on the anti-aging performance and stability of the product. When the enzymatic hydrolysis solution is not introduced, the initial protease inhibition rate of the product is relatively low, but the attenuation degree of the inhibition rate after thermal standing is relatively low. When the content of the enzymatic hydrolysis solution introduced increases, the initial inhibition rate of the product is higher, indicating that the active ingredients in the enzymatic hydrolysis solution are more helpful for enhancing the active inhibition of the product on protease.

[0075] According to the comparison of the test results of the products of Comparative Examples 3-7 and Example 1, it can be seen that the introduction of walnut extract is very crucial for the anti-aging activity of the product. If it is replaced with other common anti-aging and moisturizing plant extracts, as shown in Comparative Example 4, the initial anti-aging performance of the product will be significantly reduced. On the other hand, the anti-aging stability of the product cannot be ignored. Betaine, sunflower seed extract, and dipotassium glycyrrhizinate in the product not only play a skin care role, but also play a key role in the stability of each component of the product, especially the extracellular vesicles extract of Centella asiatica. If replaced with other similar components, as shown in Comparative Examples 5-7, the anti-aging activity of the product will decline significantly after storage. Especially for the product of Comparative Example 6, after replacing the sunflower seed extract in the product with sunflower oil, although the initial inhibition rate of the product is further improved compared to Example 1, after storage, due to the fact that sunflower oil is more likely to produce unstable substances after pyrolysis, which in turn affects the stability of other components, the inhibition rate of the product is less than 10%.

[0076] In the product of the present invention, when preparing the sunflower seed extract, considering biological activity and stability, as shown in Example 1 and Examples 7-9, when preferably using an extraction solution with a water-alcohol mass ratio of (9:1)-(7:3) for extraction, the obtained product can better balance the initial anti-aging property and storage stability.

[0077] However, even if the components of the product are appropriate, when the proportions of these key components introduced are inappropriate, as shown in Comparative Examples 8 and 9, the performance of the product in terms of anti-aging may still be poor, and the storage stability cannot be guaranteed.

[0078] Effect Example 2

[0079] To verify the actual application effects of the facial mask products of the present invention, volunteer experiments were conducted to test the products of each example and comparative example:

[0080] 180 volunteers were selected and divided into 18 groups, with 10 people in each group. The volunteers were those with healthy skin aged 25 - 35 years old and no history of skin diseases, and their skin was neutral (pre-determined through skin tests), with an equal number of men and women. Each group of volunteers was assigned a facial mask liquid product of an example or a comparative example. Taking the midline of the left and right faces as the division, after cleansing the face every day, the product was used twice a day, morning and evening. Among them, the product stored at room temperature and then opened was used on the left face normally (recorded as A), and when the product used on the right face was packaged in a packaging bag, nitrogen was filled in, and it was stored at 40°C for 20 days (if bulging occurred, air extraction and exhaust treatment were carried out) and then opened for use (recorded as B). After continuous use for 7 days, the Trans epidermal Water Loss (TEWL) value and wrinkle value of the test site on both cheeks of the volunteers before and after using the product were respectively tested using a Tewameter TM 300 instrument and a skin rapid three-dimensional imaging system PRIMOS. The change rate of trans epidermal water loss and the change rate of skin wrinkles before and after using the product were calculated, as well as the within-group average values of the two. The test results are shown in Table 4.

[0081] Change rate of trans epidermal water loss (%) = (Initial trans epidermal water loss value - Trans epidermal water loss value after use) / Initial trans epidermal water loss value × 100%;

[0082] Change rate of skin wrinkles (%) = (Initial wrinkle area - Wrinkle area after use) / Initial wrinkle area × 100%

[0083] Table 4

[0084]

[0085]

[0086] As can be seen from the test results, the product of the present invention has significant skin care activity when applied to the human face. When used for only one week, the water content increase rate of the product on the skin can reach more than 6%, and the wrinkle reduction rate can reach more than 16%. Moreover, even after high-temperature storage, the product can still exhibit good usage effects, with the water content increase rate reaching more than 5% and the skin wrinkle reduction rate reaching more than 15%, and the degree of performance attenuation being relatively low. In contrast, the product of Comparative Example 1 has poor anti-wrinkle and moisturizing effects, and each component cannot achieve an effective and rapid effect on the skin without the transportation of vesicle components; the product of Comparative Example 2 uses traditional centella asiatica extract instead of the centella asiatica extracellular vesicle extract of the present invention. Although the obtained product has a certain performance improvement compared to the product of Comparative Example 1, the improvement degree is relatively low; in the products of Comparative Examples 3 to 7, the anti-aging, moisturizing, and antioxidant components combined with the centella asiatica extracellular vesicle extract are replaced with other components with similar functions. The products cannot balance skin care activity and activity stability. For example, in the product of Comparative Example 6, the sunflower seed extract is replaced with sunflower oil. Although its initial moisturizing and anti-wrinkle effects are better, its performance attenuation is very serious after heat static storage, and the attenuation rate reaches about 50%; the dipotassium glycyrrhizinate in the product of Comparative Example 7 is replaced with ascorbic acid which also has antioxidant effects. The initial skin care performance is similar to that of the product of the Example, but the attenuation degree after heat storage is also relatively high, fully demonstrating that not any antioxidant can maintain the activity stability of the product components, especially the centella asiatica extracellular vesicle extract.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A plant essence facial mask, characterized in that, It includes a film cloth and facial mask liquid; The facial mask liquid includes the following components in parts by weight: Centella asiatica extracellular vesicle extract 0.05 - 3 parts, Chinese yam extract 0.05 - 3 parts, walnut extract 0.05 - 1 part, betaine 0.5 - 2 parts, sunflower seed extract 0.01 - 4 parts, and dipotassium glycyrrhizinate 0.05 - 0.2 parts.

2. The plant essence facial mask according to claim 1, wherein The facial mask liquid also includes 5 - 10 parts of a solvent skin conditioner; the skin conditioner includes at least one of sodium hyaluronate, allantoin, butanediol, glycerin, 1,2 - hexanediol, and 1,2 - pentanediol.

3. The plant essence facial mask according to claim 2, wherein The facial mask liquid includes 0.01 - 0.5 part of sodium hyaluronate, 0.01 - 0.5 part of allantoin, 1 - 5 parts of butanediol, 1 - 5 parts of glycerin, 0.1 - 1 part of 1,2 - hexanediol, and 0.1 - 1 part of 1,2 - pentanediol.

4. The plant essence facial mask according to claim 1, wherein The facial mask liquid also includes 0.1 - 3 parts of a plasticizer; the plasticizer includes at least one of gelatin, xanthan gum, hydroxyethyl cellulose, polyacryloyldimethyltaurine ammonium, and sodium carboxymethyl cellulose.

5. The plant essence facial mask according to claim 4, wherein The facial mask liquid includes 0.01 - 0.5 part of gelatin, 0.01 - 0.5 part of xanthan gum, 0.01 - 0.5 part of hydroxyethyl cellulose, 0.01 - 0.5 part of polyacryloyldimethyltaurine ammonium, and 0.01 - 2 parts of sodium carboxymethyl cellulose.

6. The plant essence facial mask according to claim 1, characterized in that, The facial mask liquid also contains 0.1 - 3 parts of a functional auxiliary agent; the functional auxiliary agent includes at least one of a preservative, a pH stabilizer, and a co - solvent.

7. The plant essence facial mask according to claim 1, wherein The preparation method of the Centella asiatica extracellular vesicle extract includes the following steps: After cleaning and drying Centella asiatica, perform cell wall breaking and centrifugation treatment. The obtained filtrate is filtered by a capsule filter, and the obtained secondary filtrate containing vesicles is then purified and concentrated in sequence to obtain a crude liquid; Mix the residue after the capsule filter filtration treatment with cellulase, then disperse it in water, and heat the obtained mixture to 30 - 50 °C for 1.5 - 2.5 h, filter, and mix the obtained enzymatic hydrolysate and the crude liquid according to the mass ratio of (0.5:9.5) - (1.5:8.5) to obtain the Centella asiatica extracellular vesicle extract.

8. The plant essence facial mask according to claim 1, wherein The preparation method of the sunflower seed extract includes the following steps: After shelling, washing, drying, and crushing sunflower seeds, put the obtained powder into an extraction solution for ultrasonic extraction treatment, perform solid - liquid separation, and then centrifuge and dry the obtained liquid phase to obtain the sunflower seed extract; the extraction solution includes water and ethanol, and the mass ratio of water to ethanol is (9:1) - (7:3).

9. The plant essence facial mask according to claim 1, characterized in that Based on 100 parts, the facial mask liquid includes the following components in parts by weight: Centella asiatica extracellular vesicle extract 0.6 - 0.8 parts, Chinese yam extract 0.6 - 0.8 parts, walnut extract 0.6 - 0.8 parts, betaine 1.5 - 2 parts, sunflower seed extract 3 - 4 parts, and dipotassium glycyrrhizinate 0.05 - 0.1 part.

10. The plant essence facial mask according to claim 1, characterized in that, Based on 100 parts, the facial mask liquid includes the following components in parts by weight: 0.05 - 3 parts of Centella asiatica extracellular vesicle extract, 0.05 - 3 parts of Dioscorea opposita extract, 0.05 - 1 part of walnut kernel extract, 0.5 - 2 parts of betaine, 0.01 - 4 parts of sunflower seed extract, 0.05 - 0.2 part of dipotassium glycyrrhizinate, 0.01 - 0.5 part of sodium hyaluronate, 0.01 - 0.5 part of allantoin, 1 - 5 parts of butanediol, 1 - 5 parts of glycerol, 0.01 - 0.5 part of gelatin, 0.01 - 0.5 part of xanthan gum, 0.01 - 0.5 part of hydroxyethyl cellulose, 0.01 - 0.5 part of ammonium polyacryloyldimethyltaurate, 0.01 - 2 parts of sodium carboxymethyl cellulose, 0.1 - 1 part of 1,2 - hexanediol, 0.1 - 1 part of 1,2 - pentanediol, 0.1 - 0.3 part of polydimethylsiloxane, 0.1 - 0.3 part of hydrogenated lecithin, and the balance in parts by weight of water.

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