Traditional Chinese medicine composition prepared based on enzymolysis catalysis and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly belongs to a traditional Chinese medicine composition prepared based on enzymolysis catalysis and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 10-14 parts of radix et rhizoma rhei, 5-15 parts of cortex moutan, 8-10 parts of semen persicae, 25-35 parts of semen benincasae and 7-11 parts of mirabilite. The optimal combination of extraction is optimized by adopting a monarch, minister, assistant and guide grouping method, the composite enzymolysis is added on the basis of the original process, the extraction time is shortened and the extraction efficiency is improved by optimizing the optimal enzymolysis condition, and the method has the advantages of simple instrument and equipment, low complex enzyme valence, easiness in obtaining, low extraction cost, energy conservation, environmental protection and wide application prospect. The popularization and use are easy. Besides, tests find that compared with a traditional composition prepared through water extraction, the traditional Chinese medicine composition prepared based on enzymolysis catalysis can remarkably improve weight loss, disease activity index increase and colon shortening of mice with ulcerative colitis and can relieve colon tissue pathological damage and mucous layer damage of the mice with ulcerative colitis.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition prepared based on enzymatic catalysis and its application. Background Art

[0002] Dahuang Mudanpi Decoction comes from "Synopsis of the Golden Chamber · Treatise on Pulmonary Abscess, Intestinal Abscess and Eczema, Pulse Syndromes and Treatment, Chapter 18" by Zhang Zhongjing, and is one of the representative prescriptions for treating intestinal abscess. The whole prescription is composed of rhubarb, cortex moutan, peach kernels, winter melon seeds and mirabilite, and has the effects of clearing heat and removing stasis, dissipating binds and detumescence. In the prescription, rhubarb is used as the monarch drug for clearing heat and detoxifying, removing phlegm and promoting defecation; mirabilite is used as the minister drug for softening hardness and dissipating binds, assisting rhubarb in clearing heat and detoxifying, purging and promoting defecation; peach kernels and cortex moutan are used as the assistant drugs for cooling blood and promoting blood circulation to remove stasis; winter melon seeds are used as the guiding drug for clearing dampness and heat in the intestine, discharging pus and dissipating binds. The combination of various drugs enables the heat toxin and stasis in the intestine to be discharged through defecation, so that the pus toxin can be resolved and the carbuncle swelling can be eliminated. Later physicians have explored the clinical application of Dahuang Mudanpi Decoction, and applied it to the treatment of inflammatory diseases such as ulcerative colitis, acute pancreatitis, acute appendicitis, acute leukemia and ileocecal syndrome, and achieved remarkable curative effects. Modern research has found that Dahuang Mudanpi Decoction can prevent and treat sepsis and acute pancreatitis by regulating multiple signaling pathways such as TLR4 / Myd88 / NF-κB, HMGB1 / RAGE / NF-κB and NLRP3 / caspase1.

[0003] Ulcerative colitis is an inflammatory bowel disease with complex etiology and is listed as one of the modern intractable diseases by the World Health Organization. Up to now, there is a lack of highly effective and low-toxic specific drugs for the treatment of ulcerative colitis at home and abroad, and the long-term treatment effect is not ideal. The characteristics of traditional Chinese medicine of "multiple components and multiple targets" make it show unique advantages in modern intractable diseases, so the classic famous prescription preparations passed down for thousands of years have attracted much attention. Clinically, enema with Dahuang Mudanpi Decoction can make the drug reach the lesion directly, better absorb the effective components of the drug, and achieve remarkable curative effects in the treatment of ulcerative colitis. However, enema operation requires the assistance of medical staff, and repeated enema will cause damage to the intestinal mucosa. Improper operation will also cause infection or bleeding, thus restricting the development and application of Dahuang Mudan Decoction.

[0004] The active ingredients in traditional Chinese medicine are the basis for its pharmacological effects, and the quality of the active ingredients in traditional Chinese medicine preparations mainly depends on the quality of Chinese herbal medicines and extraction and purification separation technologies. Traditionally, Dahuang Mudanpi Decoction is mainly extracted by water decoction method, which has low extraction cost and simple operation, but has deficiencies such as low extraction rate and large waste of medicinal materials. Enzymatic extraction technology can break the cell walls of plant medicinal materials, which is beneficial to the leaching and biotransformation of active substances, maximize the retention of active ingredients, promote the effective absorption and utilization of active substances by the human body, and this technology is fast, efficient, with mild reaction conditions and little pollution, having the technical advantages of large-scale production. Therefore, the Dahuang Mudanpi formula is extracted by enzyme fermentation method and prepared into dosage form compositions not limited to suppositories, etc., to improve the high efficiency and compliance of the clinical application of Dahuang Mudan Decoction.

[0005] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of suggestion that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0006] The purpose of the present invention is to provide a traditional Chinese medicine composition prepared based on enzymatic catalysis and its application.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] A traditional Chinese medicine composition is prepared from the following raw materials by mass fraction: 10 - 14 parts of rhubarb, 5 - 15 parts of cortex moutan, 8 - 10 parts of peach kernels, 25 - 35 parts of winter melon seeds, and 7 - 11 parts of mirabilite.

[0009] Preferably, it is prepared from the following raw materials by mass fraction: 12 parts of rhubarb, 10 parts of cortex moutan, 9 parts of peach kernels, 30 parts of winter melon seeds, and 9 parts of mirabilite.

[0010] The present invention also provides a preparation method of the traditional Chinese medicine composition based on enzymatic catalysis, including the following steps:

[0011] S1. Crush and sieve rhubarb and winter melon seeds, add an ethanol solution at a material-liquid ratio of 1 g : 10 - 45 mL, then add 1 - 3.5% cellulase, 0.5 - 2.5% hemicellulase, and 0.3 - 2.0% pectinase, stir evenly and place in a water bath for enzymatic hydrolysis. The enzymatic hydrolysis product is subjected to reflux extraction and filtration to obtain a first extract;

[0012] S2. Crush and sieve peach kernels and cortex moutan, add an ethanol solution at a material-liquid ratio of 1 g : 10 - 45 mL, then add 1 - 3.5% cellulase, 0.5 - 2.5% hemicellulase, and 0.3 - 2.0% pectinase, stir evenly and place in a water bath for enzymatic hydrolysis. The enzymatic hydrolysis product is subjected to reflux extraction and filtration to obtain a second extract;

[0013] S3. Mix the first extract and the second extract evenly, add mirabilite and dissolve it therein, and then concentrate and spray-dry.

[0014] Preferably, in step S1, the volume fraction of ethanol is 60%, and the material-liquid ratio is 1 g: 15 mL; in step S2, the volume fraction of ethanol is 50%, and the material-liquid ratio is 1 g: 25 mL.

[0015] Preferably, in step S1, the enzymatic hydrolysis conditions are: pH 5.0, temperature 50 °C, and enzymatic hydrolysis time 45 min; in step S2, the enzymatic hydrolysis conditions are: pH 5.5, temperature 40 °C, and enzymatic hydrolysis time 45 min.

[0016] Preferably, in step S1, the reflux extraction time is 30 min; in step S2, the reflux extraction time is 50 min.

[0017] The present invention also provides the application of the traditional Chinese medicine composition in the preparation of a drug for treating inflammatory diseases.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The present invention uses the grouping method of monarch, minister, assistant, and guide to optimize the best combination of extraction, and adds compound enzymatic hydrolysis on the basis of the original process. By optimizing the best enzymatic hydrolysis conditions, not only the extraction time is reduced, but also the extraction efficiency is improved. Moreover, the instrument and equipment used in the present invention are simple, the compound enzyme price is low and easy to obtain, the extraction cost is low, energy-saving and environmentally friendly, and it is easy to promote and use.

[0020] (2) Through experiments, it is found that compared with the composition prepared by traditional water extraction, the traditional Chinese medicine composition prepared by enzymatic hydrolysis catalysis in the present invention can significantly improve the weight loss, increased disease activity index, and colon shortening of ulcerative colitis mice, and can reduce the histopathological damage and mucus layer damage of the colon of ulcerative colitis mice. Description of the Drawings

[0021] Figure 1 It is a process diagram of the preparation method of the traditional Chinese medicine composition based on enzymatic hydrolysis catalysis of the present invention;

[0022] Figure 2 It is a diagram of the results of single-factor experiments of enzymatic hydrolysis of the preparation method of the traditional Chinese medicine composition based on enzymatic hydrolysis catalysis of the present invention;

[0023] Figure 3 It is a diagram of the therapeutic effect of each group of drugs on ulcerative colitis mice;

[0024] Figure 4 It is a diagram of H&E staining of colon tissues of each group of mice;

[0025] Figure 5It is the Alcian blue-glycogen staining map of the colon tissues of each group of mice.

[0026] Main reference numeral descriptions:

[0027] Figure 3 Among them, (a) the body weights of each group of mice on the 8th day; (b) the disease activity index of each group of mice; (c) the statistical chart of the colon lengths of each group; (d) the representative colon morphology map of each group of mice on the 8th day.

[0028] #p < 0.05, ##p < 0.01, compared with the normal group; *p < 0.05, **p < 0.01, compared with the model group; &p < 0.05, compared with the water extract composition (5 mg) group.

[0029] Figure 5 Among them, the blue-green color is the acidic mucus layer, and the purple color is the goblet cells containing both neutral and acidic mucins. Detailed implementation manners

[0030] The technical solution of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0031] Example 1

[0032] A traditional Chinese medicine composition is prepared from the following raw materials: 12 g of rhubarb, 10 g of moutan bark, 9 g of peach kernels, 30 g of winter melon seeds, and 9 g of mirabilite.

[0033] The preparation method of the above traditional Chinese medicine composition based on enzymatic hydrolysis catalysis includes the following steps:

[0034] S1. Crush rhubarb and winter melon seeds through a 60-mesh sieve, add an ethanol solution with a volume fraction of 60% at a material-liquid ratio of 1 g: 15 mL, then add 1.5% of cellulase, 0.7% of hemicellulase, and 1.2% of pectinase, stir evenly, adjust the pH to 5.0, place it in a water bath, and carry out enzymatic hydrolysis at 50 °C for 45 min; then reflux extract the enzymatic hydrolysis product for 30 min, filter, and obtain the first extract;

[0035] S2. Crush peach kernels and moutan bark through a 50-mesh sieve, add an ethanol solution with a volume fraction of 50% at a material-liquid ratio of 1 g: 25 mL, then add 1.5% of cellulase, 0.9% of hemicellulase, and 1.0% of pectinase, stir evenly, adjust the pH to 5.5, place it in a water bath, and carry out enzymatic hydrolysis at 40 °C for 45 min; then reflux extract the enzymatic hydrolysis product for 50 min, filter, and obtain the second extract;

[0036] S3. Mix the first extract and the second extract evenly, add mirabilite and dissolve it therein, concentrate and spray-dry to obtain the enzymolysis-extracted traditional Chinese medicine composition. Add glyceryl mixed fatty acid esters to make suppositories of the enzymolysis-extracted composition (containing 5 mg of the enzymolysis-extracted traditional Chinese medicine composition).

[0037] Example 2

[0038] A traditional Chinese medicine composition is prepared from the following raw materials: 12 g of Rheum officinale, 10 g of Cortex Moutan, 9 g of Semen Persicae, 30 g of Semen Benincasae and 9 g of mirabilite.

[0039] The preparation method of the above traditional Chinese medicine composition based on water extraction is as follows: Take Rheum officinale, Cortex Moutan, Semen Persicae and Semen Benincasae, soak them in 10 times the amount of water for 30 min, decoct for 40 min, filter, and take the supernatant; add 8 times the amount of water to the residue and decoct for 30 min, combine the two water decoctions, appropriately concentrate, add mirabilite to dissolve, continue to concentrate, and spray-dry to obtain the water-extracted traditional Chinese medicine composition. Add glyceryl mixed fatty acid esters to make suppositories of the water-extracted composition (containing 5 mg of the water-extracted traditional Chinese medicine composition).

[0040] Example 3 : Single-factor experiment on enzymolysis-assisted extraction

[0041] Instruments and reagents for detecting total anthraquinones in Rheum officinale, paeonol and paeoniflorin: Shimadzu high-performance liquid chromatograph (model Prominence UFLC, equipped with SPD-M20A diode array detector, LC / Labsolution chromatographic workstation, Shimadzu Corporation, Japan); one over one hundred thousandth electronic analytical balance (model MS105DU, Mettler Company); KQ-100DA desktop numerical control ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); standards of emodin, aloe-emodin, rhein, chrysophanol, physcion, paeonol and paeoniflorin were purchased from Chengdu Pufa Co., Ltd., with a purity > 99.0%; acetonitrile (chromatographic grade, Fisher Company, USA); water was self-made ultrapure water; the rest were of analytical grade.

[0042] The chromatographic method for determining emodin, paeonol and paeoniflorin in the Rheum officinale and Cortex Moutan composition refers to the detection method in the 2020 edition of the Pharmacopoeia. The mobile phase for detecting emodin is methanol-0.1% phosphoric acid water (85:15), the detection wavelength is 254 nm, the column temperature is 30 °C, the injection volume is 10 μL, the flow rate is 1.0 mL / min, and the number of theoretical plates calculated based on the emodin peak should be not less than 3000; the mobile phase for paeonol and paeoniflorin is methanol-water (45:55), the detection wavelength is 274 nm, the column temperature is 30 °C, the injection volume is 10 μL, the flow rate is 1.0 mL / min, and the number of theoretical plates calculated based on the paeonol peak should be not less than 5000.

[0043] 3.1 Optimization of cellulase addition amount

[0044] The relationship between the extraction rate of total anthraquinones from A (Rheum palmatum) and the extraction rate of paeonol from CD (Prunus persica, Paeonia suffruticosa, Benincasa hispida var. chieh-qua) and the amount of cellulase added is shown in Figure 2 a.

[0045] As can be seen from Figure 2 a, the extraction rates of total anthraquinones and paeonol first increase and then decrease with the increase in the amount of cellulase added. When the amount of cellulase added is 1.5%, the extraction rates of total anthraquinones from A (Rheum palmatum) and paeonol from CD (Prunus persica, Paeonia suffruticosa, Benincasa hispida var. chieh-qua) are the highest. When the amount of cellulase added continues to increase, the extraction rates of total anthraquinones and paeonol decrease. Therefore, the preferred amount of cellulase added is 1.5%.

[0046] 3.2 Optimization of the amount of hemicellulase added

[0047] The relationship between the extraction rate of total anthraquinones from A (Rheum palmatum) and the extraction rate of paeonol from CD (Prunus persica, Paeonia suffruticosa, Benincasa hispida var. chieh-qua) and the amount of hemicellulase added is shown in Figure 2 b.

[0048] As can be seen from Figure 2 b, with the increase in the amount of hemicellulase, the extraction rates of total anthraquinones and paeonol gradually increase. When the amount of hemicellulase added reaches 0.8%, the extraction rates of both total anthraquinones and paeonol reach the maximum. Continuing to increase the amount of hemicellulase added, the extraction rates of total anthraquinones and paeonol gradually decrease. Therefore, the preferred amount of pectinase added is 0.8%.

[0049] 3.3 Optimization of the amount of pectinase added

[0050] The relationship between the extraction rate of total anthraquinones from A (Rheum palmatum) and the extraction rate of paeonol from CD (Prunus persica, Paeonia suffruticosa, Benincasa hispida var. chieh-qua) and the amount of pectinase added is shown in Figure 2 c.

[0051] As can be seen from Figure 2 c, with the increase in the amount of pectinase added, the extraction rates of total anthraquinones and paeonol gradually increase. When the amount of pectinase added is greater than 1.0%, the extraction rates of total anthraquinones and paeonol no longer increase. Therefore, the preferred amount of pectinase added is 1.0%.

[0052] 3.4 Optimization of the enzymatic hydrolysis pH

[0053] The pH value of the system during enzymatic hydrolysis has a great influence on the enzyme activity. The relationship between the extraction rate of total anthraquinones from A (Rheum palmatum) and the extraction rate of paeonol from CD (Prunus persica, Paeonia suffruticosa, Benincasa hispida var. chieh-qua) and the enzymatic hydrolysis pH is shown in Figure 2 d.

[0054] As can be seen from Figure 2As can be seen from , when the enzymatic hydrolysis pH is 5.5, the extraction rate of total anthraquinones from A (rhubarb) is the highest, and when the enzymatic hydrolysis pH is 5.0, the extraction rate of paeonol from CD (peach kernel, cortex moutan, winter melon seed) is the highest. Therefore, the enzymatic hydrolysis pH of A (rhubarb) is determined to be 5.5, and the enzymatic hydrolysis pH of CD (peach kernel, cortex moutan, winter melon seed) is determined to be 5.0.

[0055] 3.5 Optimization of Enzymatic Hydrolysis Temperature

[0056] The activity of the enzyme is closely related to temperature. Too low or too high temperature will affect the enzyme activity and thus the extraction rate. The relationship between the extraction rate of total anthraquinones from A (rhubarb) and the extraction rate of paeonol from CD (peach kernel, cortex moutan, winter melon seed) and the enzymatic hydrolysis temperature is shown in Figure 2 e.

[0057] As can be seen from Figure 2 e, when the enzymatic hydrolysis temperature is 50 °C, the extraction rate of total anthraquinones from A (rhubarb) is the highest, and when the enzymatic hydrolysis temperature is 40 °C, the extraction rate of paeonol from CD (peach kernel, cortex moutan, winter melon seed) is the highest. As the enzymatic hydrolysis temperature continues to increase, the extraction rates of both total anthraquinones and paeonol show a downward trend. Therefore, the enzymatic hydrolysis temperature of A (rhubarb) is determined to be 50 °C, and the enzymatic hydrolysis temperature of CD (peach kernel, cortex moutan, winter melon seed) is determined to be 40 °C.

[0058] 3.6 Optimization of Enzymatic Hydrolysis Time

[0059] The enzymatic hydrolysis time determines whether the cell wall of the medicinal material can be fully destroyed, thus affecting the extraction rate. The relationship between the extraction rate of total anthraquinones from A (rhubarb) and the extraction rate of paeonol from CD (peach kernel, cortex moutan, winter melon seed) and the enzymatic hydrolysis time is shown in Figure 2 f.

[0060] As can be seen from Figure 2 f, when the enzymatic hydrolysis time is less than 40 min, as the enzymatic hydrolysis time extends, the extraction rates of both total anthraquinones and paeonol show an upward trend and reach the maximum at 40 min. Continuing to extend the enzymatic hydrolysis time shows a downward trend for the extraction rates of both total anthraquinones and paeonol. Therefore, the enzymatic hydrolysis time is determined to be 40 min.

[0061] Example 4 : Comparison of the Superiority of Enzymatic Hydrolysis Extraction of Traditional Chinese Medicine Composition and Water Extraction of Traditional Chinese Medicine Composition

[0062] 1. Materials

[0063] 1.1 Drugs and Reagents

[0064] Dextran Sulfate Sodium (DSS, molecular weight 36000 - 50000, MPBiomedicals, Canada);

[0065] Enzyme-extracted composition suppositories (containing 5 mg of enzyme-extracted Chinese medicine composition) and water-extracted composition suppositories (containing 5 mg of water-extracted Chinese medicine composition) were both made in our laboratory.

[0066] Balsalazide sodium (Hubei Baike Hendi Pharmaceutical Co., Ltd., approval number: National Medicine Standard H20030748). H&E staining kit (servicebio, item number: G1076-500ML); AB-PAS staining solution (servicebio, item number: G1049-20ML).

[0067] 1.2 Experimental animals

[0068] 50 male C57BL / 6J mice, weighing 20-22 g, were purchased from the Experimental Animal Center of Air Force Medical University [SCXK(军)2012-0007] and used in the experiment after one week of adaptive feeding. The light was artificially controlled, with 12-hour day and night alternation, indoor temperature of 23-25°C, and humidity of 55-65%.

[0069] 2. Methods

[0070] 2.1 Modeling, grouping and drug administration

[0071] The mice were randomly divided into 5 groups, 10 in each group, namely: normal group, model group, enzymatic extraction composition suppository group, water extraction composition suppository group and positive control group (administered with balsalazide sodium by intragastric administration).

[0072] The mice in the normal group drank tap water freely, and the mice in the other groups drank 3.0% DSS freely for 7 days to establish the ulcerative colitis model. At the beginning of modeling, the model group and the blank group were given blank compositions, and the other groups were given corresponding drug-containing compositions. The compositions were inserted 2 cm into the anus of the mice. After administration, the anus was gently pressed with gauze and the head was kept in a low position for 10 minutes. The drugs were administered once a day for 7 consecutive days.

[0073] 2.2 Sampling and testing

[0074] During the experiment, the fecal morphology of mice was observed and their body weight was weighed every day, and the disease activity index was scored. On the 8th day of the experiment, the colon of mice was isolated, the colon length was measured, and the intestinal contents were washed with saline and fixed in 4% paraformaldehyde solution, embedded, and sliced for H&E staining and AB-PAS staining.

[0075] 2.3 Data Analysis

[0076] All experimental data were expressed as Mean ± SD, and SPSS23.0 was used to analyze the differences between groups. When the variances were homogeneous, one-way ANOVA was used to compare the differences between multiple groups. P < 0.05 indicated a significant difference.

[0077] 3. Results

[0078] See Figures 3 - 5 .

[0079] Depend on Figure 3 It can be seen that on the 8th day of the experiment, the normal group mice had no abnormal feces and shiny fur. Compared with the normal group, the model group mice were listless, had significantly reduced body weight, had visible mucus, pus and blood in the stool, increased disease activity index, and shortened colon, which were significantly different from the normal group (P < 0.01); compared with the model group, the weight loss of mice in the enzymatic extraction composition suppository group, the water extraction composition suppository group and the positive control group was reduced, the disease activity index was reduced, and the colon atrophy was improved (P < 0.05 or P < 0.01). Compared with the water extraction composition suppository group, the enzymatic extraction composition suppository group was more effective in improving weight loss, increased disease activity index and shortened colon, with statistical differences (P < 0.05).

[0080] Depend on Figure 4 It can be seen that the colon tissue cells of the normal group mice were arranged neatly, and there was no inflammatory cell infiltration area; the colon tissue structure of the model group mice was disordered, some colon epithelial cells were replaced by inflammatory cells, there were a large number of inflammatory cell infiltration areas, crypts were destroyed, goblet cells disappeared, and submucosal edema was present; compared with the model group, the pathological damage of the colon tissue of the mice in the enzymatic extraction composition suppository group, the water extract composition suppository group and the positive control group was alleviated, but the colon of the mice in the water extract composition suppository group and the positive control group still had a large number of inflammatory cell infiltrations and submucosal edema, the pathological damage of the colon of the mice in the enzymatic extraction composition suppository group was significantly improved, with only a small part of inflammatory infiltration areas, the submucosal edema was significantly alleviated, and the tissue structure was basically restored.

[0081] The intestinal mucus barrier is the first line of defense of the intestinal mucosal barrier, which is composed of mucus proteins secreted by goblet cells. AB-PAS staining can bind to acidic mucus to show blue, and bind to neutral and acidic proteins to make goblet cells appear purple. Figure 5 It can be seen that the acidic mucus layer on the colon tissue of the normal group mice is intact, the number of goblet cells is normal, and the acidic mucus layer can be continuously secreted to ensure the integrity of the intestinal mucus barrier; the intestinal mucus barrier of the colon tissue of the model group mice is damaged, the goblet cells are reduced, the goblet cells disappear in some inflammatory areas, and the acidic mucus layer is degraded; compared with the model group, the number of goblet cells in the colon of the enzymatic extraction composition suppository group mice increases, can secrete mucus protein normally, and the acidic mucus layer on the colon is repaired. The water extract composition suppository group and the positive control group have a certain improvement effect on the colon mucus barrier, but there are still more unrepaired areas compared with the enzymatic extraction composition suppository group.

[0082] In summary, compared with the composition prepared by traditional water extraction, the traditional Chinese medicine composition prepared by the present invention based on enzymatic hydrolysis catalysis can significantly improve the weight loss, increase in disease activity index, and colon shortening of mice with ulcerative colitis, and can reduce the histopathological damage and mucus layer damage of the colon in mice with ulcerative colitis.

[0083] The foregoing description of specific exemplary embodiments of the invention has been presented for purposes of illustration and example. It is not intended to limit the invention to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical application so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention as well as various different selections and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A traditional Chinese medicine composition, characterized in that, It is prepared from the following raw materials by mass fraction: 10-14 parts of Rheum palmatum, 5-15 parts of Cortex Moutan, 8-10 parts of Prunus persica, 25-35 parts of Benincasa hispida var. chieh-qua and 7-11 parts of mirabilite.

2. The traditional Chinese medicine composition according to claim 1, wherein It is prepared from the following raw materials by mass fraction: 12 parts of Rheum palmatum, 10 parts of Cortex Moutan, 9 parts of Prunus persica, 30 parts of Benincasa hispida var. chieh-qua and 9 parts of mirabilite.

3. The preparation method of the traditional Chinese medicine composition according to any one of claims 1-2 based on enzymatic hydrolysis catalysis, characterized in that, It includes the following steps: S1. Crush and sieve Rheum palmatum and Benincasa hispida var. chieh-qua, add an ethanol solution at a material-liquid ratio of 1 g: 10-45 mL, then add 1-3.5% cellulase, 0.5-2.5% hemicellulase and 0.3-2.0% pectinase. After stirring evenly, place it in a water bath for enzymatic hydrolysis. The enzymatic hydrolysis product is subjected to reflux extraction and filtration to obtain a first extract; S2. Crush and sieve Prunus persica and Cortex Moutan, add an ethanol solution at a material-liquid ratio of 1 g: 10-45 mL, then add 1-3.5% cellulase, 0.5-2.5% hemicellulase and 0.3-2.0% pectinase. After stirring evenly, place it in a water bath for enzymatic hydrolysis. The enzymatic hydrolysis product is subjected to reflux extraction and filtration to obtain a second extract; S3. Mix the first extract and the second extract evenly, add mirabilite to dissolve it therein, and then concentrate and spray dry.

4. The preparation method according to claim 3, characterized in that, In step S1, the volume fraction of ethanol is 60%, and the material-liquid ratio is 1 g: 15 mL; In step S2, the volume fraction of ethanol is 50%, and the material-liquid ratio is 1 g: 25 mL.

5. The preparation method according to claim 3, characterized in that, In step S1, the enzymatic hydrolysis conditions are: pH 5.0, temperature 50 °C, and enzymatic hydrolysis time 45 min; In step S2, the enzymatic hydrolysis conditions are: pH 5.5, temperature 40 °C, and enzymatic hydrolysis time 45 min.

6. The preparation method according to claim 3, characterized in that, In step S1, the reflux extraction time is 30 min; in step S2, the reflux extraction time is 50 min.

7. Use of the traditional Chinese medicine composition according to any one of claims 1-2 in the preparation of a drug for treating inflammatory diseases.

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