Automatic pipe and box sealing machine for nucleic acid detection

Abstract

The invention discloses an automatic tube and box sealing machine for nucleic acid detection, and belongs to the technical field of nucleic acid detection. The automatic tube and box sealing machine for nucleic acid detection comprises a transport box, a sample placing opening and a sample taking opening are formed in the side face of the transport box in a straight line penetrating mode, the automatic tube and box sealing machine further comprises a grabbing assembly, a transport assembly, a rotating assembly, a clamping assembly and a cap screwing assembly which are arranged in the transport box, and the cap screwing assembly and the clamping assembly are used in cooperation and used for unscrewing or buckling a tube cap of a reagent tube. A closed loop is formed by the conveying device, the grabbing assembly, the clamping assembly and the cap screwing assembly, all work of nucleic acid detection is completed through cooperation of reagent tube taking, reagent tube opening, reagent tube closing and reagent tube recycling in the detection process, and unmanned operation in the nucleic acid detection process can be truly achieved; the automatic operation of nucleic acid detection is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and particularly relates to an automatic tube sealing and box sealing machine for nucleic acid detection. Background Art

[0002] Currently, for the detection of some viral diseases, it is generally determined by viral nucleic acid detection. During the nucleic acid detection process, reagent tubes, sampling cotton swabs and other tools are generally used for sampling to transfer the sample into the test tube.

[0003] For some highly infectious viruses, medical workers collecting the virus need to wear protective clothing for operation. The process of viral nucleic acid detection can be divided into a sample collection process and a sample recovery process.

[0004] In the prior art, the collection process can be replaced by a robotic arm, and most of the nucleic acid sampling robotic arms can only complete the part of sampling with a cotton swab. The robotic arm takes a sample, and after sampling, the throat swab is placed in a reagent tube. However, after sampling, the reagent tube still needs to be capped and sealed by the staff, and then the sealed reagent tube is placed in a storage box and sent to the testing center. However, during the tube sealing and box sealing processes, it is difficult for the staff to avoid contact with the samples to be detected, which will pose a certain risk of infection. Summary of the Invention

[0005] The object of the present invention is to overcome the problems in the prior art, and provide an automatic tube sealing and box sealing machine for nucleic acid detection, so as to achieve unmanned operation during the nucleic acid detection process and realize the automated operation of nucleic acid detection.

[0006] The present invention provides an automatic tube sealing and box sealing machine for nucleic acid detection, which includes a transport box. A sample placing port and a sampling port are linearly and penetratingly formed on the side surface of the transport box. An accommodation box for storing reagent tubes is placed inside the transport box. The machine further includes a grasping assembly placed inside the transport box for grasping the reagent tubes, a transport assembly placed inside the transport box and arranged above the grasping assembly for driving the grasping assembly to move along the connection line direction of the sample placing port and the sampling port, and a rotating assembly placed inside the transport box and arranged near the sample placing port. The rotating assembly includes a rotating disk, and a plurality of placement grooves are circumferentially formed on the rotating disk. The placement grooves are used for temporarily placing the reagent tubes grasped by the grasping assembly. A clamping assembly is arranged inside the transport box above the rotating disk. The clamping assembly includes a test tube clamp and a horizontally arranged fixing rod. A pair of test tube clamps are provided, and both of the pair of test tube clamps are slidably sleeved on the fixing rod. A first driving member for driving the movement of the test tube clamp is connected to each of the mutually remote sides of the pair of test tube clamps. A cap screwing assembly is placed inside the transport box. The cap screwing assembly includes a cap screwing head which is located above the clamping assembly, and a second driving member is connected to the top of the cap screwing head. The reagent tubes placed on the accommodation box are grasped by the grasping assembly, placed in the placement grooves through the transport assembly, the rotating disk rotates to turn the reagent tubes towards the clamping assembly side, the cap screwing assembly and the clamping assembly cooperate to unscrew the tube caps of the reagent tubes, a robotic arm places a throat swab into the reagent tubes through the sample placing port, then the cap screwing assembly screws the reagent tubes tightly again, and the reagent tubes are put back into the accommodation box through the cooperation of the grasping assembly and the transport assembly.

[0007] Preferably, the transport assembly includes a transport frame, a toothed belt, a first gear, a second gear, a first motor, guide rails and columns. A pair of guide rails are provided. Columns are fixedly connected to the bottoms of the horizontal two ends of each of the pair of guide rails. The bottoms of several columns are fixedly connected to the transport box. The toothed belt is sleeved on the guide rails. The transport frame is slidably connected to the guide rails. The first motor is fixedly connected to one side of the guide rails, and the output end is fixedly connected to the first gear. The second gear is rotatably connected to the transport frame. Both the first gear and the second gear are engaged with the toothed belt. The transport frame moves horizontally on the pair of guide rails.

[0008] Preferably, the grasping assembly includes a grasping head, a cylinder and a bearing frame. The bearing frame is horizontally slidably connected to the bottom of the transport frame. The cylinder is vertically arranged and fixedly connected inside the bearing frame. The end of the cylinder is fixedly connected to the grasping head.

[0009] Preferably, the rotating assembly further includes a fifth motor, a third gear, and a fourth gear. The fifth motor is vertically and fixedly connected inside the transport box. The output end of the fifth motor is fixedly connected to the third gear. The fourth gear meshes with one side of the third gear. The support rod is vertically arranged, with one end rotatably connected to the transport box and the other end fixedly connected to the rotating disk. The fourth gear is fixedly sleeved on the support rod.

[0010] Preferably, the first driving member further includes a first fixing frame, a double-headed screw rod, and a second motor. The first fixing frame is provided in a pair, and the pair of first fixing frames are horizontally and fixedly connected to the side wall of the transport box. The double-headed screw rod is rotatably connected between the pair of first fixing frames. A pair of threads on the double-headed screw rod are arranged in opposite directions. The second motor is fixedly connected to one side of the first fixing frame. The output end of the second motor is fixedly connected to the double-headed screw rod. A pair of test tube clamps are threadedly sleeved on the double-headed screw rod.

[0011] Preferably, the second driving member includes a second fixing frame, a third motor, a threaded rod, a nut, a connecting plate, and a fourth motor. The second fixing frame is fixedly connected inside the transport box. The third motor is vertically and fixedly connected to the bottom of the second fixing frame. The output end of the third motor is fixedly connected to the threaded rod. The nut is threadedly sleeved on the threaded rod. The connecting plate is horizontally and fixedly connected to one side of the nut. The fourth motor is vertically and fixedly connected to the connecting plate. The output end of the fourth motor is fixedly connected to the cap screwing head. A sliding rod is horizontally fixedly connected to the side of the nut away from the connecting plate. One end of the sliding rod away from the connecting plate is vertically slidably connected to the inner side wall of the transport box.

[0012] Preferably, a stabilizing disk is fixedly connected to the top of the support rod. A plurality of stabilizing grooves are formed on the side edge of the stabilizing disk. The plurality of stabilizing grooves are arranged in one-to-one correspondence with the plurality of placing grooves and have the same axis.

[0013] Preferably, the grasping head is made of medical clean rubber, and the inner diameter of the grasping head is the same as the outer diameter of the anti-slip rough line on the outer side of the reagent tube cap.

[0014] Preferably, the cap screwing head is made of rubber material and has an interference fit with the reagent tube cap.

[0015] Preferably, support seats are fixedly connected to the bottom corners of the transport box.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: An automatic tube sealing and box sealing machine for nucleic acid detection of the present invention is used in conjunction with a sampling robot. The transport component moves the provided grasping component above the reagent tubes in the storage box. The reagent tubes are grasped by the grasping component. At this time, the first motor rotates in reverse, and under the combined action of the first gear, the second gear, and the toothed belt, the transport frame moves towards the clamping component. When the grasping component moves the reagent tubes to one side of the clamping component, the reagent tubes are stably clamped by the clamping component. At this time, the tube caps of the reagent tubes are unscrewed by the provided cap screwing component. The sampling robot puts the collected test samples into the reagent tubes, and then the cap screwing component tightens the tube caps of the reagent tubes. At this time, the reagent tubes are grasped by the grasping component again, and in cooperation with the transport component, the reagent tubes containing the test samples are transported back to the storage box. The sampling process of the present invention forms a closed loop, and each component cooperates with each other to work, achieving no human participation in the whole process, saving labor and avoiding the risk of infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the internal structure of the transport box of the present invention.

[0018] Figure 2 It is a schematic diagram of the structure when the rotating component, the clamping component, and the cap screwing component of the present invention cooperate.

[0019] Figure 3 It is a schematic diagram of the clamping component structure of the present invention.

[0020] Figure 4 It is a schematic diagram of the overall structure of the present invention.

[0021] Description of the reference numerals: 1. Transport box; 2. Storage box; 3. Reagent tube; 4. Stable groove; 5. Transport component; 51. Transport frame; 52. Toothed belt; 53. First gear; 54. Second gear; 55. First motor; 56. Guide rail; 57. Column; 6. Grasping component; 61. Carrier frame; 62. Cylinder; 63. Grasping head; 7. Clamping component; 71. First fixing frame; 72. Double-headed screw; 73. Second motor; 74. Test tube clamp; 75. Fixed rod; 8. Cap screwing component; 81. Second fixing frame; 82. Third motor; 83. Threaded rod; 84. Nut; 85. Connecting plate; 86. Fourth motor; 87. Cap screwing head; 9. Rotating component; 91. Fifth motor; 92. Third gear; 93. Fourth gear; 94. Support rod; 95. Rotating disk; 96. Placing groove; 10. Support seat; 11. Sample placing port; 12. Stable disk. DETAILED DESCRIPTION OF THE INVENTION

[0022] The following combines the attached Figures 1 to 4, for the purpose of making the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. Based on the described embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should have the ordinary meaning understood by those with ordinary skills in the field to which the present invention pertains.

[0023] The terms "first", "second", and similar terms used in the specification and claims of this patent application for invention do not denote any order, quantity, or importance, but are only used to distinguish different components. Terms such as "including" or "comprising" mean that the elements or objects appearing before this term cover the elements or objects listed after this term and their equivalents, without excluding other elements or objects. Terms such as "inside", "outside", "above", "below", "far", "near", "front", "rear", etc. are only used to represent relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly. The drawings in the present invention are not strictly drawn to actual scale, and the specific dimensions and quantities of each structure can be determined according to actual needs. The drawings described in the present invention are only schematic diagrams of the structure.

[0024] An automatic tube sealing and box sealing machine for nucleic acid detection provided by the present invention, as Figures 1 to 4 shown, includes a transport box 1. A sample placement opening 11 and a sampling opening are linearly and penetratingly provided on the side of the transport box 1. An accommodation box 2 for storing reagent tubes 3 is placed inside the transport box 1. It further includes a grasping assembly 6 placed inside the transport box 1 for grasping the reagent tubes 3. A transport assembly 5 is placed inside the transport box 1 and is arranged above the grasping assembly 6, and is used to drive the grasping assembly 6 to move along the connection line direction of the sample placement opening 11 and the sampling opening. A rotating assembly 9 is placed inside the transport box 1 and is arranged near the sample placement opening 11. The rotating assembly 9 includes a rotating disk 95, and a plurality of placement slots 96 are circumferentially provided on the rotating disk 95. The placement slots 96 are used for temporarily placing the reagent tubes 3 grasped by the grasping assembly 6. A clamping assembly 7 is arranged inside the transport box 1 above the rotating disk 95. The clamping assembly 7 includes test tube clamps 74 and fixed rods 75. A pair of test tube clamps 74 are provided. Both of the pair of test tube clamps 74 are slidably sleeved on the fixed rods 75. A first driving member for driving the movement of the test tube clamps 74 is connected to each side of the pair of test tube clamps 74 that are away from each other. A cap screwing assembly 8 is placed inside the transport box 1. The cap screwing assembly 8 includes a cap screwing head 87. The cap screwing head 87 is located above the clamping assembly 7. A second driving member is connected to the top of the cap screwing head 87. The cap screwing assembly 8 is used in cooperation with the clamping assembly 7 to unscrew or fasten the caps of the reagent tubes 3. The reagent tube 3 placed on the storage box 2 is grasped by the grasping component 6, placed in the placement groove 96 through the transportation component 5, the rotating disk 95 rotates to turn the reagent tube 3 towards the clamping component 7, the cap screwing component 8 and the clamping component 7 cooperate to unscrew the tube cap of the reagent tube 3, the robotic arm places the throat swab into the reagent tube 3 through the sample placing port 11, then the reagent tube 3 is tightened again by the cap screwing component 8, and the reagent tube 3 is placed back into the storage box 2 through the cooperation of the grasping component 6 and the transportation component 5.

[0025] An automatic tube sealing and box sealing machine for nucleic acid detection of the present invention is used in cooperation with a robotic arm. The transportation component 5 moves the provided grasping component 6 above the reagent tube 3 in the storage box 2. The reagent tube 3 is grasped by the grasping component 6. At this time, the first motor 55 rotates in reverse, and under the cooperative action of the first gear 53, the second gear 54 and the toothed belt 52, the transportation frame 51 moves towards the clamping component 7. When the grasping component 6 moves the reagent tube 3 to one side of the clamping component 7, the reagent tube 3 is stably clamped by the clamping component 7. At this time, the tube cap of the reagent tube 3 is unscrewed by the provided cap screwing component 8. The robotic arm places the collected test sample into the reagent tube 3, and then the tube cap of the reagent tube 3 is tightened by the cap screwing component 8. At this time, the reagent tube 3 is grasped by the grasping component 6 again, and in cooperation with the transportation component 5, the reagent tube 3 containing the test sample is transported back to the storage box 2. The sampling process of the present invention forms a closed loop, and each component cooperates with each other to work, achieving a full process without human participation, which not only saves labor but also avoids the risk of infection.

[0026] Preferably, as Figures 1 to 3 shown, the transportation component 5 includes a transportation frame 51, a toothed belt 52, a first gear 53, a second gear 54, a first motor 55, guide rails 56 and columns 57. Columns 57 are fixedly connected to the bottom of both horizontal ends of a pair of guide rails 56, and the bottoms of several columns 57 are fixedly connected to the transportation box 1. The toothed belt 52 is sleeved on the guide rails 56, the transportation frame 51 is slidably connected to the guide rails 56, the first motor 55 is fixedly connected to one side of the guide rails 56, and the output end is fixedly connected to the first gear 53. The second gear 54 is rotatably connected to the transportation frame 51. Both the first gear 53 and the second gear 54 are engaged with the toothed belt 52. The transportation frame 51 moves horizontally on a pair of guide rails 56, driving the reagent tube 3 to move in the horizontal direction.

[0027] The first motor 55 drives the provided first gear 53 to rotate. The first gear 53 is engaged with the toothed belt 52. Under the action of the second gear 54, the transportation frame 51 moves horizontally on a pair of guide rails 56, thereby driving the grasping component 6 to move.

[0028] Preferably, as Figures 1 to 2As shown, the grasping assembly 6 includes a grasping head 63, a cylinder 62, and a carrier 61. The carrier 61 is slidably connected to the bottom of the transport rack 51. The cylinder 62 is vertically arranged and fixedly connected inside the carrier 61. The end of the cylinder 62 is fixedly connected to the grasping head 63. The grasping head 63 is made of medical clean rubber. The inner diameter of the grasping head 63 is the same as the outer diameter of the anti-slip rough line on the outer side of the cap of the reagent tube 3. The cap screwing head 87 is made of rubber and is in interference fit with the cap of the reagent tube 3. At the bottom corners of the transport box 1, support seats 10 are fixedly connected.

[0029] In this embodiment, the carrier 61 is slidably arranged on a pair of guide rails 56. When the carrier 61 moves above the reagent tube 3, the grasping head 63 is pushed down by the cylinder 62 to grasp the reagent tube 3 placed in the storage box 2. In the present invention, the grasping head 63 is made of medical clean rubber, and the inner diameter of the grasping head 63 is designed to be the same as the outer diameter of the anti-slip rough line on the outer side of the cap of the reagent tube 3, ensuring that the sample in the reagent tube 3 will not be contaminated after sampling, not only ensuring that the reagent tube 3 does not fall but also avoiding damage to the reagent tube 3 due to excessive force. The grasping head 63 can open and close automatically.

[0030] Preferably, as Figures 1 to 2 shown, the rotating assembly 9 further includes a fifth motor 91, a third gear 92, and a fourth gear 93. The fifth motor 91 is vertically and fixedly connected inside the transport box 1. The output end of the fifth motor 91 is fixedly connected to the third gear 92. The fourth gear 93 is engaged with one side of the third gear 92. The support rod 94 is vertically arranged, with one end rotatably connected to the transport box 1 and the other end fixedly connected to the rotating disk 95. The fourth gear 93 is fixedly sleeved on the support rod 94.

[0031] In this embodiment, the transport box 1 supports the provided fifth motor 91. The fifth motor 91 drives the provided third gear 92 to rotate. The third gear 92 is engaged with the fourth gear 93, thereby driving the support rod 94 to rotate. The support rod 94 drives the provided rotating disk 95 to rotate. The transport assembly 5 places the transported reagent tube 3 in the placement groove 96 opened on the rotating disk 95 to standardize the verticality of the reagent tube 3 and can complete the work of the reagent tube 3 in the working box, ensuring that subsequent work such as cap screwing and throat swab collection can be carried out smoothly.

[0032] Preferably, as Figures 1 to 3 shown, the first driving member includes a first fixing frame 71, a double-headed screw 72, and a second motor 73. There are a pair of the first fixing frames 71, and the pair of first fixing frames 71 are horizontally and fixedly connected to the side wall of the transport box 1. The double-headed screw 72 is rotatably connected between the pair of first fixing frames 71. A pair of threads on the double-headed screw 72 are arranged in opposite directions. The second motor 73 is fixedly connected to one side of the first fixing frame 71. The output end of the second motor 73 is fixedly connected to the double-headed screw 72. A pair of test tube clamps 74 are threadedly sleeved on the double-headed screw 72.

[0033] In this embodiment, the transport box 1 supports the provided clamping assembly 7. When the reagent tube 3 is transported to one side of the clamping assembly 7 through the transport assembly 5, the second motor 73 drives the provided double-headed screw 72 to rotate, so that a pair of test tube clamps 74 sleeved thereon move under the guidance of the fixed rod 75 to stably clamp the reagent tube 3, and cooperate with the cap screwing assembly 8 to unscrew the tube cap of the reagent tube 3, facilitating the robotic arm to place the sampled cotton swab into the opened reagent tube 3.

[0034] Preferably, as Figures 1 to 3 shown, the second driving member includes a second fixing frame 81, a third motor 82, a threaded rod 83, a nut 84, a connecting plate 85 and a fourth motor 86. The second fixing frame 81 is fixedly connected inside the transport box 1. The third motor 82 is vertically and fixedly connected to the bottom of the second fixing frame 81. The output end of the third motor 82 is fixedly connected to the threaded rod 83. The nut 84 is threadedly sleeved on the threaded rod 83. The connecting plate 85 is horizontally and fixedly connected to one side of the nut 84. The fourth motor 86 is vertically and fixedly connected to the connecting plate 85. The output end of the fourth motor 86 is fixedly connected to the cap screwing head 87. A sliding rod is horizontally fixedly connected to the side of the nut 84 away from the connecting plate 85. One end of the sliding rod away from the connecting plate 85 is vertically slidably connected to the inner side wall of the transport box 1.

[0035] In this embodiment, the transport box 1 supports the provided second fixing frame 81, and the second fixing frame 81 supports the provided third motor 82. After the clamping assembly 7 clamps the reagent tube 3, the third motor 82 rotates to make the nut 84 stably move downward on the threaded rod 83 with the assistance of the sliding rod. Here, the threaded rod 83 and the nut 84 are used to precisely control the cap screwing head 87 in the vertical direction. When the cap screwing head 87 covers the tube cap of the reagent tube 3, the fourth motor 86 drives the provided cap screwing head 87 to rotate to unscrew the tube cap of the reagent tube 3, and then the third motor 82 moves the cap screwing head 87 upward. The cap screwing head 87 realizes the fixation and rotation of the tube cap of the reagent tube 3 through interference fit.

[0036] Preferably, as Figures 1 to 3 shown, a stabilizing disk 12 is fixedly connected to the top of the support rod 94. A plurality of stabilizing grooves 4 are formed on the side of the stabilizing disk 12. The plurality of stabilizing grooves 4 are arranged in one-to-one correspondence with the plurality of placement grooves 96 and their axes coincide.

[0037] The stability of the placed reagent tube 3 is ensured through the stabilizing disk 12, and because the stabilizing grooves 4 are arranged in one-to-one correspondence with the placement grooves 96 and their axes coincide, it is ensured that the reagent tube 3 is vertically placed to avoid the reagent tube 3 from tipping over.

[0038] The usage method of the automatic tube capping and box sealing machine for nucleic acid detection of the present invention is as follows: The first gear 53 is driven by the first motor 55 to rotate. The first gear 53 meshes with the toothed belt 52. Under the action of the second gear 54, the transport rack 51 moves horizontally on a pair of guide rails 56, and the set gripping assembly 6 is moved above the reagent tube 3 in the storage box 2. The cylinder 62 is used to push the set gripper head 63 downward to grip the reagent tube 3 placed in the storage box 2. In the present invention, the gripper head 63 is made of medical clean rubber, and the inner diameter of the gripper head 63 is the same as the outer diameter of the anti-slip rough line on the outer side of the tube cap of the reagent tube 3, ensuring that the sample in the reagent tube 3 will not be contaminated after sampling. It not only ensures that the reagent tube 3 does not fall but also avoids damage to the reagent tube 3 due to excessive force. After gripping the reagent tube 3, the first motor 55 rotates in reverse, and under the combined action of the first gear 53, the second gear 54 and the toothed belt 52, the transport rack 51 moves towards the clamping assembly 7. The gripped reagent tube 3 is placed in the placement groove 96 in the rotary disk 95. The second motor 73 drives the set double-headed screw 72 to rotate, so that a pair of test tube clamps 74 sleeved thereon move under the guidance of the fixed rod 75 to stably clamp the reagent tube 3. After the clamping assembly 7 clamps the reagent tube 3, the third motor 82 rotates to make the nut 84 stably move downward on the threaded rod 83 with the assistance of the sliding rod. Here, the threaded rod 83 and the nut 84 are used to precisely control the vertical movement of the cap screwing head 87. When the cap screwing head 87 covers the tube cap of the reagent tube 3, the fourth motor 86 drives the set cap screwing head 87 to rotate to unscrew the tube cap of the reagent tube 3. The mechanical arm places the collected sample into the reagent tube 3 through the sample placing port 11, thus completing the sampling. The reagent tube 3 is gripped by the gripper head 63, placed in the placement groove 96 through the transport assembly 5. The rotary disk 95 rotates to turn the reagent tube 3 towards the clamping assembly 7. The cap unscrewing assembly 8 and the clamping assembly 7 cooperate to unscrew the tube cap of the reagent tube 3. The mechanical arm places the throat swab into the reagent tube 3 through the sample placing port 11, then the cap unscrewing assembly 8 screws the reagent tube 3 tightly again, and the reagent tube 3 is placed in the storage box 2 through the cooperation of the gripping assembly 6 and the transport assembly 5.

[0039] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automatic tube sealing and box sealing machine for nucleic acid detection, characterized in that, It includes a transport box. A layout opening and a sampling opening are linearly and penetratingly provided on the side of the transport box. An accommodation box for storing reagent tubes is placed inside the transport box. It also includes the following components placed inside the transport box: A grasping component for grasping the reagent tubes; A transport component arranged above the grasping component and used to drive the grasping component to move along the direction of the connection line between the layout opening and the sampling opening; A rotating component arranged near the layout opening. The rotating component includes a rotating disk. A plurality of placement grooves are circumferentially provided on the rotating disk, and the placement grooves are used for temporarily placing the reagent tubes grasped by the grasping component; A clamping component located above the rotating disk. The clamping component includes a test tube clamp and a horizontally arranged fixing rod. A pair of test tube clamps are provided. Both of the pair of test tube clamps are slidably sleeved on the fixing rod. A first driving part for driving the movement of the test tube clamp is connected to the mutually distant sides of the pair of test tube clamps; A cap-twisting component including a cap-twisting head. The cap-twisting head is located above the clamping component, and a second driving part is connected to the top of the cap-twisting head; The reagent tubes stored on the accommodation box are grasped by the grasping component, placed in the placement grooves through the transport component, the rotating disk rotates to turn the reagent tubes to the side of the clamping component, the cap-twisting component and the clamping component cooperate to unscrew the caps of the reagent tubes, the robotic arm places the throat swab into the reagent tubes through the layout opening, then the cap-twisting component screws the reagent tubes tightly again, and the reagent tubes are put back into the accommodation box through the cooperation of the grasping component and the transport component.

2. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, wherein, The transport component includes a transport frame, a toothed belt, a first gear, a second gear, a first motor, guide rails and columns. A pair of guide rails are provided. Columns are fixedly connected to the bottoms of the horizontal two ends of the pair of guide rails. The bottoms of several columns are fixedly connected to the transport box. The toothed belt is sleeved on the guide rails. The transport frame is slidably connected to the guide rails. The first motor is fixedly connected to one side of the guide rails, and the output end is fixedly connected to the first gear. The second gear is rotatably connected to the transport frame. Both the first gear and the second gear are engaged with the toothed belt, and the transport frame moves horizontally on the pair of guide rails.

3. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 2, wherein The grasping component includes a grasping head, a cylinder and a bearing frame. The bearing frame is horizontally slidably connected to the bottom of the transport frame. The cylinder is vertically arranged and fixedly connected inside the bearing frame. The end of the cylinder is fixedly connected to the grasping head.

4. An automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, characterized in that, The rotating component further includes a fifth motor, a third gear and a fourth gear. The fifth motor is vertically and fixedly connected inside the transport box. The output end of the fifth motor is fixedly connected to the third gear. The fourth gear is engaged with one side of the third gear. A support rod is vertically arranged, one end is rotatably connected to the transport box, and the other end is fixedly connected to the rotating disk. The fourth gear is fixedly sleeved on the support rod.

5. An automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, characterized in that, The first driving member includes a first fixing frame, a double-headed screw rod, and a second motor. The first fixing frame is provided in a pair, and the pair of first fixing frames are horizontally and fixedly connected to the side wall of the transport box. The double-headed screw rod is rotatably connected between the pair of first fixing frames. A pair of threads on the double-headed screw rod are arranged in opposite directions. The second motor is fixedly connected to one side of the first fixing frame, and the output end of the second motor is fixedly connected to the double-headed screw rod. A pair of test tube clamps are threadedly sleeved on the double-headed screw rod.

6. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, wherein The second driving member includes a second fixing frame, a third motor, a threaded rod, a nut, a connecting plate, and a fourth motor. The second fixing frame is fixedly connected inside the transport box. The third motor is vertically and fixedly connected to the bottom of the second fixing frame. The output end of the third motor is fixedly connected to the threaded rod. The nut is threadedly sleeved on the threaded rod. The connecting plate is horizontally and fixedly connected to one side of the nut. The fourth motor is vertically and fixedly connected to the connecting plate. The output end of the fourth motor is fixedly connected to the cap screwing head. A sliding rod is horizontally fixedly connected to the side of the nut away from the connecting plate. The end of the sliding rod away from the connecting plate is vertically and slidably connected to the inner side wall of the transport box.

7. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 4, wherein A stabilizing disc is fixedly connected to the top of the support rod. A plurality of stabilizing grooves are formed on the side of the stabilizing disc. The plurality of stabilizing grooves are arranged in one-to-one correspondence with the plurality of placement grooves and have the same axis.

8. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 3, wherein, The grasping head is made of medical clean rubber, and the inner diameter of the grasping head is the same as the outer diameter of the anti-slip rough line on the outer side of the reagent tube cap.

9. An automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, characterized in that, The cap screwing head is made of rubber material and is in interference fit with the reagent tube cap.

10. The automatic tube sealing and box sealing machine for nucleic acid detection according to claim 1, characterized in that, Support seats are fixedly connected to the bottom corners of the transport box.

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