Tricholoma matsutake-containing health-care medicinal liquor for enhancing human immune function and preparation method thereof

Abstract

The invention discloses tricholoma matsutake-containing health-care medicinal liquor capable of enhancing a human immune function and a preparation method thereof, relates to the technical field of health-care products, and aims to solve the problems that the alcohol concentration cannot be adjusted according to preparation requirements and the extraction efficiency of effective components is low. According to the key points of the technical scheme, the medicinal liquor is prepared from tricholoma matsutake, fructus lycii, dried longan, radix astragali seu hedysari, lucid ganoderma, radix angelicae sinensis, liquorice root, honey and base liquor, and the medicinal liquor is prepared through the matched use of extraction preparation, three-stage gradient degree reduction extraction and filtration and seasoning. The tricholoma matsutake, fructus lycii, dried longan, radix astragali seu hedysari, lucid ganoderma, radix angelicae sinensis, liquorice root, honey and base liquor are selected; three-stage gradient degree reduction extraction, cellulase and pulsed electric field combination are adopted, the optimal dissolution conditions of different components are matched, the alcohol concentration is adjusted according to preparation requirements, the extraction rate of the immunocompetence components of the medicinal liquor is increased, and the medicinal effect is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care products, specifically a health care medicated wine containing Tricholoma matsutake and enhancing human immune function, and relates to a health care medicated wine containing Tricholoma matsutake and enhancing human immune function and a preparation method thereof. Background Art

[0002] Medicated wine is a drink formed by using wine as a solvent to soak a variety of Chinese herbal medicines for a certain period of time. Medicated wine has medicinal and health care functions and is often used to promote blood circulation, enhance immunity, relieve fatigue, etc.

[0003] Immunity refers to the ability of the human body to resist diseases and infections, which is achieved by the immune system through identifying and eliminating pathogens (such as bacteria, viruses, fungi, etc.). Poor immunity may make people more susceptible to diseases or suffer from other health problems.

[0004] The Chinese invention patent with the authorization announcement number CN101537081B discloses a health care medicated wine, especially a Tricholoma matsutake health care medicated wine with anti-cancer and human immunity enhancing functions and a preparation method thereof, belonging to the technical field of health care products. It is prepared from the following raw materials according to the weight part ratio: 500 - 800 of 40 - 65-degree white liquor, 2 - 6 of Tricholoma matsutake, 1 - 4 of Chinese wolfberry, 1 - 3 of ginseng, 1 - 4 of Ganoderma lucidum, 1 - 4 of Chinese date, 0.5 - 2 of longan, and 1 - 5 of medical stone. The medicated wine of the present invention has a unique flavor, is fragrant, and has a mellow taste. It contains crude protein, crude fat, crude fiber, and in addition, also contains various vitamins and various amino acids. Through clinical experiment verification, its anti-cancer and immunity enhancing effects are excellent.

[0005] In the preparation process of the medicated wine in the above patent, the air-dried raw materials are directly put into a wine vat or wine storage tank containing the above-mentioned weight of white liquor, and then sealed for 30 - 3000 days, and it is impossible to adjust the alcohol concentration according to the preparation requirements, resulting in the problem of low extraction efficiency of active ingredients.

[0006] In summary, therefore, the present invention provides a health care medicated wine containing Tricholoma matsutake and enhancing human immune function and a preparation method thereof to solve the above problems. Summary of the Invention

[0007] Aiming at the deficiencies existing in the prior art, the purpose of the present invention is to provide a health care medicated wine containing Tricholoma matsutake and enhancing human immune function and a preparation method thereof. The advantage is that the three-stage gradient alcohol concentration reduction extraction method is adopted, which can adjust the alcohol concentration according to the preparation requirements and has a high extraction efficiency of active ingredients.

[0008] To achieve the above purpose, the present invention provides the following technical solutions:

[0009] A health medicated wine containing matsutake mushrooms for enhancing human immune function, comprising matsutake mushrooms, Chinese wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base wine. The matsutake mushrooms, Chinese wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey are proportioned by weight as follows: 25 - 30 g of matsutake mushrooms, 15 g of Chinese wolfberries, 20 g of dried longans, 15 g of astragalus membranaceus, 8 - 10 g of ganoderma lucidum, 6 - 8 g of angelica sinensis, 5 g of licorice, 50 - 60 g of honey and 1000 ml of base wine.

[0010] By adopting the above technical solution, a health medicated wine containing matsutake mushrooms for enhancing human immune function is prepared through the combined use of matsutake mushrooms, Chinese wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base wine.

[0011] A preparation method of a health medicated wine containing matsutake mushrooms for enhancing human immune function specifically includes the following steps:

[0012] Step 1, extraction preparation: Prepare raw materials and containers according to the formula, and pretreat the raw materials;

[0013] Step 2, three-stage gradient reduction extraction: Prepare the medicated wine with matsutake mushrooms, Chinese wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base wine;

[0014] Step 3, filtration and flavoring: Filter, flavor and store the prepared medicated wine.

[0015] By adopting the above technical solution, the medicated wine is made through the combined use of extraction preparation, three-stage gradient reduction extraction and filtration and flavoring.

[0016] The present invention is further set as: The extraction preparation in Step 1 specifically includes the following steps:

[0017] Step S1, raw material preparation: Weigh matsutake mushrooms, Chinese wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base wine according to the weight for standby;

[0018] Step S2, raw material pretreatment: Freeze the matsutake mushrooms at -18°C for 24 hours and then slice them into slices with a thickness of 2 mm, perform honey-roasting treatment on astragalus membranaceus and angelica sinensis, bake the Chinese wolfberries and dried longans at 40°C at low temperature until the water content < 10%, and ultramicro-pulverize the ganoderma lucidum;

[0019] Step S3, container treatment: Select a brown glass container and spray and disinfect it with 75% alcohol.

[0020] By adopting the above technical solution, it is convenient to prepare the medicated wine.

[0021] The present invention is further set as: The three-stage gradient reduction extraction in Step 2 specifically includes the following steps:

[0022] Step N1, High-alcohol extraction: Pour liquor with an alcohol content of 52-55 degrees into a glass container, add matsutake, ganoderma lucidum, and angelica sinensis, seal and place in the dark at an environment temperature of 25±2°C for soaking for 0-7 days. Oscillate 3 times a day at a rotation speed of 200 rpm, with each oscillation lasting for 5 minutes. On the 3rd day, add cellulase with a concentration of 0.01%. After adding, place the sample in a water bath at 40°C and continuously heat for 2 hours;

[0023] Step N2, Medium-alcohol extraction: Adjust the alcohol concentration to 45-48 degrees by adding distilled water, add astragalus membranaceus, wolfberry, and dried longan. Seal and place in the dark at an environment temperature of 20±2°C for soaking from the 8th to the 18th day. Oscillate 2 times a week at a rotation speed of 300 rpm, with each oscillation lasting for 10 minutes. On the 10th day, use pulsed electric field treatment. The intensity of the pulsed electric field is 5 kV / cm, each pulse lasts for 10 milliseconds, and it is repeated 10 times in total;

[0024] Step N3, Low-alcohol stabilization: Adjust the alcohol concentration to 38-40 degrees by adding distilled water, add licorice and honey. Seal and place in the dark at an environment temperature of 4-8°C for soaking from the 19th to the 28th day. Gently shake once a day. On the 25th day, use vacuum distillation technology with a vacuum degree of -0.08 MPa and distill at a temperature of 60°C.

[0025] By adopting the above technical solution, by matching the optimal dissolution conditions of different components and combining cellulase and pulsed electric field, the extraction rate of the total active ingredients is increased by 52%.

[0026] The present invention is further configured as: The filtration and flavoring in the third step specifically include the following steps:

[0027] Step M1, Fine filtration: First, coarsely filter the medicinal liquor after soaking with a 100-mesh gauze, and then finely filter it with a 0.45-μm microporous membrane to remove all solid substances and ensure that the medicinal liquor is clear;

[0028] Step M2, Flavor blending: Add 0.05% natural peppermint essential oil to improve the aftertaste, and adjust the sweetness to 8-10°Brix with honey;

[0029] Step M3, Sterilization and filling: After pasteurizing the flavored medicinal liquor at 62°C for 30 minutes, fill it with nitrogen and fill it into a glass container. Store it in a dry and cool place, seal it for storage, and avoid direct sunlight.

[0030] By adopting the above technical solution, the required medicinal liquor is prepared.

[0031] The present invention is further configured such that: after the high-alcohol extraction in step N1 is completed, the content of triterpenoids in matsutake is monitored to ensure that the content of triterpenoids in matsutake ≥ 0.5 mg / ml; after the medium-alcohol extraction in step N2 is completed, the total amount of β-glucan + astragaloside IV is monitored to ensure that the total amount of β-glucan + astragaloside IV ≥ 2.0 mg / ml; after the low-alcohol stabilization in step N3 is completed, the total number of colonies is monitored to ensure that the total number of colonies < 100 CFU / ml.

[0032] By adopting the above technical solution, through the detection of each stage, the normal progress of the preparation work of the medicinal liquor is ensured.

[0033] To sum up, the beneficial technical effects of the present invention are as follows:

[0034] Select matsutake, wolfberry, dried longan, astragalus, ganoderma lucidum, angelica, licorice, honey and base liquor, and through three-stage gradient reduction extraction, the combined use of cellulase and pulsed electric field, match the optimal dissolution conditions of different components, and adjust the alcohol concentration according to the preparation requirements to improve the extraction rate of immune active components of the medicinal liquor and enhance the medicinal effect. Specific Embodiments

[0035] The following further elaborates on the present invention in detail.

[0036] The present invention discloses a health care medicinal liquor containing matsutake for enhancing human immune function, which includes matsutake, wolfberry, dried longan, astragalus, ganoderma lucidum, angelica, licorice, honey and base liquor. The weight ratio of matsutake, wolfberry, dried longan, astragalus, ganoderma lucidum, angelica, licorice, honey is 25 - 30 g of matsutake, 15 g of wolfberry, 20 g of dried longan, 15 g of astragalus, 8 - 10 g of ganoderma lucidum, 6 - 8 g of angelica, 5 g of licorice, 50 - 60 g of honey and 1000 ml of base liquor.

[0037] The present invention discloses a preparation method of a health care medicinal liquor containing matsutake for enhancing human immune function, which specifically includes the following steps:

[0038] Step 1. Extraction preparation: Prepare raw materials and containers according to the formula, and perform pretreatment on the raw materials;

[0039] Step 2. Three-stage gradient reduction extraction: Prepare the medicinal liquor with matsutake, wolfberry, dried longan, astragalus, ganoderma lucidum, angelica, licorice, honey and base liquor;

[0040] Step 3. Filtration and flavoring: Filter, flavor and store the prepared medicinal liquor.

[0041] Furthermore, the extraction preparation in step 1 specifically includes the following steps:

[0042] Step S1, Raw material preparation: Weigh matsutake, wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base liquor according to weight for standby;

[0043] Step S2, Raw material pretreatment: Freeze the matsutake at -18°C for 24 hours and then slice it into pieces with a thickness of 2 mm. Conduct honey-roasting treatment on astragalus membranaceus and angelica sinensis, low-temperature dry wolfberries and dried longans at 40°C until the water content < 10%, and ultramicro-pulverize ganoderma lucidum;

[0044] Step S3, Container treatment: Select a brown glass container and spray it with 75% alcohol for disinfection.

[0045] Furthermore, the three-stage gradient reduction extraction in step two specifically includes the following steps:

[0046] Step N1, High-alcohol extraction: Pour liquor with a degree of 52 - 55 into a glass container, add matsutake, ganoderma lucidum and angelica sinensis, seal and place it in a dark environment at a temperature of 25 ± 2°C, soak for 0 - 7 days, shake it 3 times a day, with a rotation speed of 200 rpm and each time lasting for 5 minutes. Add cellulase with a concentration of 0.01% on the 3rd day. After adding, put the sample into a water bath at 40°C and continuously heat for 2 hours;

[0047] After the high-alcohol extraction is completed, monitor the content of triterpenoids in matsutake to ensure that the content of triterpenoids in matsutake ≥ 0.5 mg / ml;

[0048] Step N2, Medium-alcohol extraction: Adjust the alcohol concentration to 45 - 48 degrees by adding distilled water, add astragalus membranaceus, wolfberries and dried longans, seal and place it in a dark environment at a temperature of 20 ± 2°C, soak for the 8th - 18th day, shake it 2 times a week, with a rotation speed of 300 rpm and each time lasting for 10 minutes. Use pulsed electric field treatment on the 10th day, the intensity of the pulsed electric field is 5 kV / cm, each pulse lasts for 10 milliseconds, and repeat it 10 times in total;

[0049] After the medium-alcohol extraction is completed, monitor the total amount of β-glucan + astragaloside IV to ensure that the total amount of β-glucan + astragaloside IV ≥ 2.0 mg / ml;

[0050] Step N3, Low-alcohol stabilization: Adjust the alcohol concentration to 38 - 40 degrees by adding distilled water, add licorice and honey, seal and place it in a dark environment at a temperature of 4 - 8°C, soak for the 19th - 28th day, gently shake it once a day. Use vacuum distillation technology on the 25th day, with a vacuum degree of -0.08 MPa and distill at a temperature of 60°C;

[0051] After the low-alcohol stabilization is completed, monitor the total number of colonies to ensure that the total number of colonies < 100 CFU / ml.

[0052] Furthermore, the filtration and flavoring in step three specifically include the following steps:

[0053] Step M1, Fine Filtration: After the soaking of the medicinal liquor is completed, first coarsely filter it with a 100-mesh gauze, and then finely filter it with a 0.45-μm microporous membrane to remove all solid substances and ensure the clarity of the medicinal liquor.

[0054] Step M2, Flavor Blending: Add 0.05% natural peppermint essential oil to improve the aftertaste, and adjust the sweetness to 8 - 10 °Brix with honey.

[0055] Step M3, Sterilization and Bottling: After pasteurizing the flavored medicinal liquor at 62 °C for 30 minutes, fill it with nitrogen and bottle it into glass containers. Store it in a dry and cool place, seal it, and avoid direct sunlight.

[0056] Example 1

[0057] Weigh 30 g of matsutake, 15 g of wolfberries, 20 g of dried longans, 15 g of astragalus membranaceus, 10 g of ganoderma lucidum, 8 g of angelica sinensis, 5 g of licorice, 60 g of honey, and 1000 ml of base liquor. Wash the weighed matsutake, wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, and licorice, put them into a clean container, add the base liquor to ensure that the liquor can completely cover all the materials, add honey according to taste, seal the container, and place it in a cool and dry place for about 2 - 4 weeks. During this period, shake it regularly once to help the components dissolve. After the soaking is completed, filter the liquor with a gauze or filter net to remove solid substances, and pour the filtered medicinal liquor into a clean glass bottle to obtain the medicinal liquor.

[0058] Example 2

[0059] Weigh 30 g of matsutake, 15 g of wolfberries, 20 g of dried longans, 15 g of astragalus membranaceus, 10 g of ganoderma lucidum, 8 g of angelica sinensis, 5 g of licorice, 60 g of honey, and 1000 ml of base liquor. For the weighed matsutake, wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey, and base liquor, prepare the medicinal liquor through the combined use of extraction preparation, three-stage gradient alcohol reduction extraction, and filtration and flavor blending.

[0060] Example 3

[0061] Weigh 30 g of matsutake, 15 g of wolfberries, 20 g of dried longans, 15 g of astragalus membranaceus, 10 g of ganoderma lucidum, 8 g of angelica sinensis, 5 g of licorice, 60 g of honey, and 1000 ml of base liquor. For the weighed matsutake, wolfberries, dried longans, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey, and base liquor, prepare the medicinal liquor through the combined use of extraction preparation, three-stage gradient alcohol reduction extraction, and filtration and flavor blending. However, do not add cellulase during the high-alcohol extraction process and do not perform pulsed electric field treatment during the medium-alcohol extraction process to obtain the medicinal liquor.

[0062] By preparing health care medicinal wines with different medicinal effects in Example 1, Example 2, and Example 3, detecting the active ingredients in the medicinal wines, and detecting the immune effects, the active ingredient parameters include β-glucan, astragaloside IV, and lycium barbarum polysaccharide, and the immune effect indicators include NK cell activity, macrophage phagocytosis rate, and IgG secretion amount.

[0063]

[0064] Table 1

[0065] It can be seen from Table 1 that by comparing Example 2 with Example 3, it can be concluded that by adding cellulase, due to the enzymatic hydrolysis destroying the chitin structure of the cell wall, the extraction rate of Tricholoma matsutake β-glucan is increased from 1.9 mg / ml to 2.8 mg / ml, an increase of 47.4%. By promoting the release through pulsed electric field-induced instantaneous cell membrane perforation, the dissolution amount of astragaloside IV is increased from 0.05 mg / ml to 0.07 mg / ml, an increase of 40%.

[0066] By comparing Example 1 with Example 3, it can be concluded that by adopting three-stage gradient reduction extraction, even without synergistic technologies (enzyme / electric field assistance), β-glucan is still 58.3% higher than the traditional method.

[0067] It can be concluded from this that Example 2 adopts three-stage gradient reduction extraction + enzyme / electric field assistance, and the active ingredients and efficacy indicators are comprehensively leading. If the equipment is limited, Example 3 only adopting three-stage gradient reduction extraction is still superior to the traditional process in Example 1. It can be known that the scientifically optimized extraction process can increase the immune active ingredients in the medicinal wine by more than twice.

[0068] The examples of this specific implementation manner are all preferred examples of the present invention, and do not limit the protection scope of the present invention accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of the present invention shall be covered within the protection scope of the present invention.

Claims

1. A health medicated wine containing Tricholoma matsutake and enhancing human immune function, comprising Tricholoma matsutake, Chinese wolfberry, dried longan, astragalus membranaceus, ganoderma lucidum, angelica sinensis, licorice, honey and base wine, characterized in that: The matsutake, wolfberry, dried longan, astragalus root, ganoderma lucidum, angelica sinensis, licorice root, and honey are proportioned by weight as follows: 25-30 g of matsutake, 15 g of wolfberry, 20 g of dried longan, 15 g of astragalus root, 8-10 g of ganoderma lucidum, 6-8 g of angelica sinensis, 5 g of licorice root, 50-60 g of honey, and 1000 ml of base liquor.

2. A preparation method of a health medicated wine containing Tricholoma matsutake for enhancing human immune function, characterized in that, The preparation method is applied to the health medicated wine as described in Claim 1, and specifically includes the following steps: Step 1. Extraction preparation: Prepare the raw materials and containers according to the formula, and pre-treat the raw materials; Step 2. Three-stage gradient reduction extraction: Prepare the medicated wine with matsutake, wolfberry, dried longan, astragalus root, ganoderma lucidum, angelica sinensis, licorice root, honey, and base liquor; Step 3. Filtration and flavoring: Filter, flavor, and store the prepared medicated wine.

3. The preparation method of a health medicated wine containing matsutake and enhancing human immune function according to claim 2, characterized in that, The extraction preparation in Step 1 specifically includes the following steps: Step S1. Raw material preparation: Weigh matsutake, wolfberry, dried longan, astragalus root, ganoderma lucidum, angelica sinensis, licorice root, honey, and base liquor according to the weight for standby; Step S2. Raw material pre-treatment: Freeze the matsutake at -18°C for 24 hours and then slice it into slices with a thickness of 2 mm. Perform honey-roasting treatment on astragalus root and angelica sinensis. Bake the wolfberry and dried longan at 40°C at low temperature until the water content is <10%. Ultrafinely pulverize the ganoderma lucidum; Step S3. Container treatment: Select a brown glass container and spray and disinfect it with 75% alcohol.

4. The preparation method of a health care medicinal wine containing Tricholoma matsutake and enhancing human immune function according to claim 2, characterized in that, The three-stage gradient reduction extraction in Step 2 specifically includes the following steps: Step N1. High-alcohol extraction: Pour 52-55-degree liquor into a glass container, add matsutake, ganoderma lucidum, and angelica sinensis, seal and place it in a dark place at a temperature of 25±2°C, soak for 0-7 days, shake it 3 times a day, with a rotation speed of 200 rpm and each time lasting for 5 minutes. Add cellulase with a concentration of 0.01% on the 3rd day. After adding, put the sample into a water bath at 40°C and continuously heat for 2 hours; Step N2. Medium-alcohol extraction: Adjust the alcohol concentration to 45-48 degrees by adding distilled water, add astragalus root, wolfberry, and dried longan, seal and place it in a dark place at a temperature of 20±2°C, soak for the 8th - 18th day, shake it 2 times a week, with a rotation speed of 300 rpm and each time lasting for 10 minutes. Use pulsed electric field treatment on the 10th day, with the intensity of the pulsed electric field being 5 kV / cm, each pulse lasting for 10 milliseconds, and repeating a total of 10 times; Step N3. Low-alcohol stabilization: Adjust the alcohol concentration to 38-40 degrees by adding distilled water, add licorice root and honey, seal and place it in a dark place at a temperature of 4-8°C, soak for the 19th - 28th day, gently shake it once a day. Use vacuum distillation technology on the 25th day, with a vacuum degree of -0.08 MPa and distill at a temperature of 60°C.

5. The preparation method of a health medicated wine containing Tricholoma matsutake and enhancing human immune function according to claim 3, characterized in that, The filtration and flavoring in Step 3 specifically includes the following steps: Step M1. Fine filtration: First, coarsely filter the soaked medicated wine with a 100-mesh gauze, and then finely filter it with a 0.45-μm microporous membrane to remove all solid substances and ensure that the medicated wine is clear; Step M2. Flavor blending: Add 0.05% natural mint essential oil to improve the aftertaste, and adjust the sweetness to 8-10°Brix with honey; Step M3, sterilization and filling: After pasteurizing the flavored medicinal liquor at 62°C for 30 minutes, fill it with nitrogen and then fill it into glass containers, store it in a dry and cool place, seal it, and avoid direct sunlight.

6. The preparation method of a health medicated wine containing Tricholoma matsutake and enhancing human immune function according to claim 4, characterized in that, After the high-alcohol extraction in step N1 is completed, monitor the content of triterpenoids in matsutake to ensure that the content of triterpenoids in matsutake ≥ 0.5 mg / ml.

7. The preparation method of a health care medicinal liquor containing Tricholoma matsutake and enhancing human immune function according to claim 4, characterized in that, After the medium-alcohol extraction in step N2 is completed, monitor the total amount of β-glucan + astragaloside IV to ensure that the total amount of β-glucan + astragaloside IV ≥ 2.0 mg / ml.

8. The preparation method of a health medicated wine containing Tricholoma matsutake and enhancing human immune function according to claim 4, characterized in that, After the low-alcohol stabilization in step N3 is completed, monitor the total number of colonies to ensure that the total number of colonies < 100 CFU / ml.

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