Cistanche deserticola cornel wine suitable for men to drink and preparation method thereof

Abstract

The invention relates to the technical field of drinks, in particular to cistanche deserticola and cornus officinalis wine suitable for men to drink and a preparation method thereof, and the cistanche deserticola and cornus officinalis wine is prepared from the following raw materials in parts by weight: cistanche deserticola, cornus officinalis, red dates and a wine base. The wine base is prepared from kaoliang spirit of 42 degrees; by infiltrating the medicinal materials and combining a specific supercritical carbon dioxide extraction technology, fat-soluble impurities in the medicinal materials can be efficiently removed, and meanwhile, effective components in the cistanche, the dogwood and the red dates are reserved to the greatest extent. Compared with a traditional simple soaking extraction mode, the desert cistanche and cornel wine can contain more substances beneficial to the health of men, so that the health-care effect is remarkably enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of beverages, and particularly to a cistanche and cornel wine suitable for men to drink and a preparation method thereof. Background Art

[0002] With the continuous improvement of people's health awareness, the demand for alcoholic beverages is no longer limited to simple taste enjoyment. More and more consumers are beginning to pursue alcoholic beverages with certain health care effects.

[0003] For the male group, the demand for alcoholic beverages is usually relatively high. However, with the continuous increase of age, in the fast-paced modern life, the body is prone to problems such as fatigue and decreased immunity, and conventional alcoholic beverages may continue to exacerbate such problems.

[0004] Traditional health care wines usually have some problems. On the one hand, many health care wine formulas are relatively simple, just simply soaking several common medicinal materials in wine, and the extraction rate of active ingredients is relatively low, making it difficult to fully exert the health care effect of the medicinal materials.

[0005] On the other hand, due to the random placement of various medicinal materials, the taste of some health care wines is not good. This is because of the special taste of the medicinal materials themselves, making the taste of the wine liquid bitter and strange, and difficult to be accepted by consumers, greatly reducing the willingness of consumers to drink.

[0006] Therefore, it is of great significance to develop a cistanche and cornel wine suitable for men to drink. Summary of the Invention

[0007] Aiming at the problems existing in the prior art, the present invention provides a cistanche and cornel wine suitable for men to drink and a preparation method thereof.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A cistanche and cornel wine suitable for men to drink, which is made from the following raw materials in parts by weight:

[0010] Cistanche deserticola 5 - 8 parts, cornel 5 - 8 parts, red dates 3 - 5 parts, wine base 150 - 160 parts;

[0011] The wine base is prepared from 42-degree sorghum wine;

[0012] The parameters of the sorghum wine: total acid calculated as acetic acid: 0.46 g / L;

[0013] Total ester calculated as ethyl acetate: 1.51 g / L;

[0014] Solids: ≤1.0 g / L;

[0015] Methanol content: ≤0.6 g / L.

[0016] A preparation process of Cistanche salsa and Macrocarpium officinale wine suitable for men to drink, comprising the following steps:

[0017] (1) Medicinal material treatment:

[0018] First, soak Macrocarpium officinale and red dates in ethanol with a volume fraction of 50% for 12 - 14 hours, then filter and crush to obtain crushed materials;

[0019] Perform supercritical carbon dioxide extraction on the obtained crushed materials to remove fat-soluble impurities and obtain pretreated medicinal materials;

[0020] Ferment Cistanche salsa to obtain fermented Cistanche salsa;

[0021] (2) Biphasic extraction:

[0022] Perform biphasic extraction on the above-obtained pretreated medicinal materials. Specifically, add 8 - 10 times the mass of water to the pretreated medicinal materials, perform reflux extraction at 100°C for 3 times, each time for 40 minutes, combine the 3 water extracts, concentrate to a relative density of 1.0, then add an ethanol solution with a mass fraction of 90%, keep the ethanol content in the mixed system at 65 - 68%, stir for 30 minutes, let stand for 22 - 24 hours, then centrifuge and separate, take the supernatant, add fermented Cistanche salsa to the supernatant, mix evenly to obtain a composite supernatant;

[0023] (3) Microcapsule embedding treatment:

[0024] First, mix β-cyclodextrin and stevioside in a mass ratio of 10:0.8 - 1.2 to obtain a preliminary mixture;

[0025] Mix the preliminary mixture with the above-obtained composite supernatant in a mass ratio of 1:5 - 6 to obtain a β-cyclodextrin inclusion complex, then perform secondary coating with ethyl cellulose, the addition amount of ethyl cellulose is 70% of the mass of β-cyclodextrin, and then perform spray drying to form a microcapsule with a core-shell structure;

[0026] (4) Aging process:

[0027] Mix the wine base with the above microcapsules, then add 0.05 - 0.8 wt% of xanthan gum as a suspending agent, perform ultrasonic dispersion for 10 minutes, finally store in a constant temperature cellar for 6 months, and then take out.

[0028] As a further technical solution, the soaking temperature in step (1) is 55 - 60°C;

[0029] The preparation method of fermented Cistanche deserticola is as follows: Clean Cistanche deserticola with clear water; Cut the washed Cistanche deserticola into slices with a thickness of 1 mm, and place them in an oven at 60 °C to dry until the moisture content is lower than 10%; Use a pulverizer to pulverize the dried Cistanche deserticola to 80 mesh to obtain Cistanche deserticola powder; Add water 10 times the mass of the Cistanche deserticola powder to the Cistanche deserticola powder, then add 0.5 wt% of cellulase, mix evenly, and then place the mixture in a water bath environment at 42 °C and continuously enzymolyze for 4 hours to obtain an enzymolysis solution; Inoculate Aspergillus niger into the enzymolysis solution, control the temperature at 30 - 32 °C, keep the humidity at 65 - 70%, and carry out fermentation for 72 hours to obtain a fermentation broth; Use an ethanol solution with a mass fraction of 70% and carry out ultrasonic-assisted extraction on the fermentation broth at 50 °C for a total of 3 times, with each extraction time being 30 minutes; After the extraction is completed, combine the extraction solutions of the 3 times to obtain a composite solution; Let the composite solution pass through a D101 macroporous resin column for chromatography, and use an ethanol solution with a mass fraction of 50% as an eluent for elution; Collect the eluate and carry out freeze-drying treatment to obtain fermented Cistanche deserticola.

[0030] As a further technical solution, the parameters of supercritical carbon dioxide extraction in step (1) are:

[0031] The extraction pressure is 22 - 23 MPa;

[0032] The extraction temperature is 45 - 48 °C.

[0033] As a further technical solution, the rotation speed of centrifugal separation in step (2) is 8000 r / min.

[0034] As a further technical solution, the spray drying parameters in step (3) are:

[0035] The inlet air temperature is 175 °C, the outlet air temperature is 80 °C, the feeding speed is 20 - 25 ml / min, and the atomization pressure is 0.15 - 0.18 MPa.

[0036] As a further technical solution, the frequency of ultrasonic dispersion in step (4) is 30 - 35 kHz.

[0037] As a further technical solution, nitrogen is filled into the cellar every month during the cellar storage period in step (4) to replace oxygen.

[0038] As a further technical solution, the temperature of constant-temperature cellar storage in step (4) is 15 - 18 °C.

[0039] As a further technical solution, the temperature of constant-temperature cellar storage in step (4) is 16 °C.

[0040] Compared with the prior art, the beneficial effects of the present invention are:

[0041] The present invention can efficiently remove fat-soluble impurities in the medicinal materials by soaking the medicinal materials and combining a specific supercritical carbon dioxide extraction technology, while retaining the effective ingredients in Cistanche deserticola, Cornus officinalis and red dates to the greatest extent. Compared with the traditional simple soaking extraction method, the technical solution of the present invention can make the Cistanche deserticola and Cornus officinalis wine contain more substances that are beneficial to men's health, thereby significantly enhancing the health care effect.

[0042] The two-phase extraction process further ensures the comprehensive extraction of the active ingredients of the medicinal materials. Compared with a single extraction method, the types of active ingredients extracted are significantly increased.

[0043] The present invention is processed by microcapsule embedding, β-cyclodextrin and stevioside are mixed in a specific ratio to form a β-cyclodextrin inclusion compound, and then ethyl cellulose is used for secondary coating and spray drying. This process can not only effectively cover up the bitter taste of the medicinal material itself, but also make the wine have a unique flavor. The Cistanche and Cornus officinalis wine prepared by the process of the present invention has a more mellow and soft taste, a more intense and lasting aroma, and has obvious improvements in taste and flavor.

[0044] The wine base of the present invention selects 42-proof sorghum wine with specific parameters, and its total acid, total ester, solid and methanol contents all meet strict standards. It is integrated with the medicinal material extract to further optimize the taste and quality of the wine, making the taste of the wine more coordinated and balanced.

[0045] The present invention can promote the aging reaction of the wine by storing it in a constant temperature cellar for 6 months and introducing nitrogen to replace oxygen every month during the storage period, making the taste of the wine more mature and stable, while effectively preventing the oxidation and deterioration of the wine and extending the shelf life of the product.

[0046] Cistanche deserticola is known as the "desert ginseng". It is rich in various amino acids, phenylethanol glycosides and other ingredients. It can replenish kidney yang, benefit essence and blood, and moisten the intestines and promote bowel movements. It has a good effect on common male symptoms such as kidney yang deficiency, soreness of waist and knees. After a specific fermentation process, the antioxidant effect of the Cistanche cornus wine is greatly improved.

[0047] Cornus officinalis contains effective ingredients such as loganin and morroniside, which have the effects of nourishing the liver and kidneys, astringing the semen and preventing deficiency, can enhance human immunity, and have positive significance for regulating body functions.

[0048] Red dates are rich in vitamin C, iron, etc., which have the effects of tonifying qi, nourishing blood and calming the nerves. They can help men relieve fatigue and replenish qi and blood.

[0049] The invention makes the cistanche, cornus and red dates have significant health-care effects through the scientific combination of cistanche, cornus and red dates.

[0050] Instruction Manual

[0051] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION

[0052] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] The cistanche and cornus officinalis wine of the present invention is prepared from the following raw materials in parts by weight: 5-8 parts of cistanche, 5-8 parts of cornus officinalis, 3-5 parts of red dates, and 150-160 parts of wine base. The wine base is prepared from 42-proof kaoliang wine, and the kaoliang wine has specific parameters: the total acid calculated as acetic acid is 0.46 g / L; the total ester calculated as ethyl acetate is 1.51 g / L; the solid content is ≤1.0 g / L; and the methanol content is ≤0.6 g / L.

[0054] The preparation process of the Cistanche and Cornus officinalis wine of the present invention comprises the following steps:

[0055] Medicinal material processing:

[0056] First, Cistanche deserticola, Cornus officinalis, and red dates are soaked in 50% ethanol by volume for 12-14 hours. In the present invention, the soaking temperature is preferably 55-60°C. After the soaking is completed, the crushed material is filtered and crushed to obtain the crushed material. The obtained crushed material is subjected to supercritical carbon dioxide extraction treatment to remove fat-soluble impurities to obtain pretreated medicinal materials. In the present invention, the parameters of supercritical carbon dioxide extraction are: extraction pressure 22-23MPa; extraction temperature 45-48°C;

[0057] Fermenting the Cistanche deserticola to obtain fermented Cistanche deserticola;

[0058] The preparation method of fermented Cistanche deserticola is as follows: Wash Cistanche deserticola with clear water; Cut the washed Cistanche deserticola into slices with a thickness of 1 mm, and place them in an oven at 60 °C to dry until the moisture content is lower than 10%; Use a pulverizer to pulverize the dried Cistanche deserticola to 80 meshes to obtain Cistanche deserticola powder; Add water 10 times the mass of the Cistanche deserticola powder to the Cistanche deserticola powder, then add 0.5 wt% of cellulase, mix evenly, and then place the mixture in a water bath environment at 42 °C and continuously enzymolyze for 4 hours to obtain an enzymolysis solution; Inoculate Aspergillus niger into the enzymolysis solution, control the temperature at 30 - 32 °C, keep the humidity at 65 - 70%, and carry out fermentation for 72 hours to obtain a fermentation broth; Use an ethanol solution with a mass fraction of 70% to carry out ultrasonic-assisted extraction on the fermentation broth at 50 °C, extract 3 times in total, and each extraction time is 30 minutes; After the extraction is completed, combine the extraction solutions of the 3 times to obtain a composite solution; Let the composite solution pass through a D101 macroporous resin column for chromatography, and use an ethanol solution with a mass fraction of 50% as an eluent for elution; Collect the eluate and carry out freeze-drying treatment to obtain fermented Cistanche deserticola.

[0059] Two-phase extraction:

[0060] Perform two-phase extraction treatment on the pretreated medicinal materials obtained above. Specifically, add water with a mass 8 - 10 times that of the pretreated medicinal materials, carry out reflux extraction treatment at 100 °C for 3 times, each time for 40 min, combine the water extraction solutions of the 3 times, and concentrate to a relative density of 1.0.

[0061] Then add an ethanol solution with a mass fraction of 90% to keep the ethanol content in the mixed system reach 65 - 68%, stir for 30 min, stand for 22 - 24 hours, then carry out centrifugal separation, take the supernatant, add fermented Cistanche deserticola to the supernatant, mix evenly to obtain a composite supernatant. In the present invention, the rotation speed of centrifugal separation is preferably 8000 r / min.

[0062] Microencapsulation treatment:

[0063] First, mix β-cyclodextrin and stevioside evenly according to a mass ratio of 10:0.8 - 1.2 to obtain a preliminary mixture.

[0064] Mix the preliminary mixture with the composite supernatant obtained above according to a mass ratio of 1:5 - 6 to obtain a β-cyclodextrin inclusion complex, then perform secondary coating with ethyl cellulose, the addition amount of ethyl cellulose is 70% of the mass of β-cyclodextrin, and then carry out spray drying to form a microcapsule with a core-shell structure. In the present invention, the spray drying parameters are: the inlet air temperature is 175 °C, the outlet air temperature is 80 °C, the feeding speed is 20 - 25 ml / min, and the atomization pressure is 0.15 - 0.18 MPa.

[0065] Aging process:

[0066] Mix the wine base with the above microcapsules, then add 0.05 - 0.8 wt% xanthan gum as a suspending agent, and disperse ultrasonically for 10 min. In the present invention, the ultrasonic dispersion frequency is preferably 30 - 35 kHz.

[0067] Finally, store in a constant temperature cellar for 6 months, then take it out, and that's it. In the present invention, nitrogen is filled monthly during the cellar storage to displace oxygen. The temperature for constant temperature cellar storage is preferably 15 - 18°C, more preferably 16°C.

[0068] The following are specific examples:

[0069] Example 1

[0070] In this example, the prepared Cistanche and Cornus officinalis wine is prepared according to the following steps:

[0071] Medicinal material treatment: Weigh 5 parts of Cistanche deserticola, 5 parts of Cornus officinalis, and 3 parts of red dates, soak them in 50% (volume fraction) ethanol at 55°C for 12 hours, filter and then crush to obtain a crushed material. Perform supercritical carbon dioxide extraction on the crushed material under the conditions of an extraction pressure of 22 MPa and an extraction temperature of 45°C to obtain pretreated medicinal materials; ferment Cistanche deserticola to obtain fermented Cistanche deserticola;

[0072] The preparation method of fermented Cistanche deserticola is as follows: Wash Cistanche deserticola with clear water; cut the washed Cistanche deserticola into 1 - mm - thick slices, place them in an oven at 60°C and dry until the moisture content is less than 10%; use a pulverizer to crush the dried Cistanche deserticola to 80 meshes to obtain Cistanche deserticola powder; add 10 times the mass of water to the Cistanche deserticola powder, then add 0.5 wt% cellulase, mix evenly, and then place the mixture in a water bath environment at 42°C and continuously enzymolyze for 4 hours to obtain an enzymolysis solution; inoculate Aspergillus niger into the enzymolysis solution, control the temperature at 30°C, keep the humidity at 65%, and perform fermentation for 72 hours to obtain a fermentation broth; use a 70% (mass fraction) ethanol solution to perform ultrasonic - assisted extraction on the fermentation broth in an environment at 50°C, extract 3 times in total, with each extraction duration of 30 minutes; after the extraction is completed, combine the extraction solutions of the 3 times to obtain a composite solution; let the composite solution pass through a D101 macroporous resin column for chromatography, and use a 50% (mass fraction) ethanol solution as an eluent for elution; collect the eluate and perform freeze - drying treatment to obtain fermented Cistanche deserticola.

[0073] Two - phase extraction: Add 8 times the mass of water to the pretreated medicinal materials, reflux and extract at 100°C for 3 times, each time for 40 min, combine the water extraction solution and concentrate it to a relative density of 1.0. Add a 90% (mass fraction) ethanol solution to make the ethanol content in the mixed system reach 65%, stir for 30 min, stand for 22 hours, centrifuge and separate at a speed of 8000 r / min, take the supernatant, add fermented Cistanche deserticola to the supernatant, mix evenly to obtain a composite supernatant.

[0074] Microcapsule embedding treatment: β-cyclodextrin and stevioside were mixed uniformly at a mass ratio of 10:0.8 to obtain a preliminary mixture. The preliminary mixture was mixed with the composite supernatant at a mass ratio of 1:5 to obtain a β-cyclodextrin inclusion compound, and ethyl cellulose was added in an amount of 70% of the mass of β-cyclodextrin for secondary coating, and spray-dried under the conditions of inlet air temperature of 175°C, outlet air temperature of 80°C, feed speed of 20ml / min, and atomization pressure of 0.15MPa to form microcapsules.

[0075] Aging process: 150 parts of wine base are mixed with microcapsules, 0.05wt% of xanthan gum is added as a suspending agent, ultrasonically dispersed at a frequency of 30kHz for 10 minutes, and stored at a constant temperature of 15°C for 6 months. During this period, nitrogen is filled in every month to replace oxygen, and the Cistanche and Cornus officinalis wine is obtained.

[0076] Example 2

[0077] In this embodiment, the following steps are followed to prepare the Cistanche and Cornus officinalis wine:

[0078] Medicinal material processing: Weigh 6 parts of Cistanche deserticola, 6 parts of Cornus officinalis, and 4 parts of red dates, soak them in 50% ethanol by volume at 58°C for 13 hours, filter and grind to obtain a crushed material. The crushed material is subjected to supercritical carbon dioxide extraction at an extraction pressure of 22.5MPa and an extraction temperature of 46°C to obtain a pretreated medicinal material; ferment Cistanche deserticola to obtain fermented Cistanche deserticola;

[0079] The preparation method of fermented Cistanche deserticola comprises the following steps: washing Cistanche deserticola with clean water; cutting the washed Cistanche deserticola into 1 mm thick slices, placing the slices in an oven at 60°C to dry until the moisture content is less than 10%; crushing the dried Cistanche deserticola to 80 meshes using a grinder to obtain Cistanche deserticola powder; adding 10 times the weight of water to the Cistanche deserticola powder, and then adding 0.5wt% of cellulase, mixing the mixture evenly, placing the mixture in a water bath at 42°C, and continuously performing enzymolysis for 4 hours to obtain an enzymolysis solution; inoculating Aspergillus niger into the enzymolysis solution, and controlling the temperature at 3 1℃, humidity is maintained at 68%, fermentation is carried out for 72 hours, and fermentation liquid is obtained; using 70% by mass ethanol solution, at 50℃ environment, ultrasonic-assisted extraction is carried out on the fermentation liquid, a total of 3 extractions are carried out, and each extraction time is 30 minutes; after the extraction is completed, the 3 extractions are combined to obtain a composite liquid; the composite liquid is chromatographed through a D101 macroporous resin column, and eluted with 50% by mass ethanol solution as an eluent; the eluate is collected, and freeze-dried to obtain fermented Cistanche deserticola.

[0080] Biphasic extraction: Add water with a mass 9 times that of the pretreated medicinal materials to the pretreated medicinal materials, reflux and extract at 100 °C for 3 times, 40 min each time. Combine the water extracts and concentrate to a relative density of 1.0. Add an ethanol solution with a mass fraction of 90%, so that the ethanol content in the mixed system reaches 66%. Stir for 30 min, let stand for 23 hours, centrifuge and separate at a speed of 8000 r / min, take the supernatant, add fermented cistanche deserticola to the supernatant, and mix evenly to obtain a composite supernatant.

[0081] Microcapsule embedding treatment: Mix β-cyclodextrin and stevioside evenly according to a mass ratio of 10:1 to obtain a preliminary mixture. Mix the preliminary mixture and the composite supernatant according to a mass ratio of 1:5.5 to obtain a β-cyclodextrin inclusion complex, and perform secondary coating with ethyl cellulose with an addition amount of 70% of the mass of β-cyclodextrin. Spray drying is carried out under the conditions of an inlet air temperature of 175 °C, an outlet air temperature of 80 °C, a feeding speed of 22 ml / min, and an atomization pressure of 0.16 MPa to form microcapsules.

[0082] Aging process: Mix 155 parts of wine base with the microcapsules, add 0.4 wt% of xanthan gum as a suspending agent, disperse ultrasonically at a frequency of 32 kHz for 10 min, store in a constant temperature cellar at 16 °C for 6 months, during which nitrogen is filled every month to displace oxygen, and take out to obtain cistanche deserticola and dogwood wine.

[0083] Example 3

[0084] In this example, cistanche deserticola and dogwood wine is prepared according to the following steps:

[0085] Medicinal material treatment: Weigh 8 parts of cistanche deserticola, 8 parts of cornel, and 5 parts of red dates, soak them in ethanol with a volume fraction of 50% at 60 °C for 14 hours, filter and then crush to obtain a crushed material. Perform supercritical carbon dioxide extraction treatment on the crushed material under the conditions of an extraction pressure of 23 MPa and an extraction temperature of 48 °C to obtain pretreated medicinal materials; ferment cistanche deserticola to obtain fermented cistanche deserticola;

[0086] The preparation method of fermented Cistanche deserticola is as follows: Wash Cistanche deserticola with clean water; Cut the washed Cistanche deserticola into slices with a thickness of 1 mm, and place them in an oven at 60 °C to dry until the moisture content is lower than 10%; Use a pulverizer to pulverize the dried Cistanche deserticola to 80 meshes to obtain Cistanche deserticola powder; Add 10 times the mass of water to the Cistanche deserticola powder, then add 0.5 wt% of cellulase, mix evenly, and then place the mixture in a water bath environment at 42 °C and continuously enzymolyze for 4 hours to obtain an enzymolysis solution; Inoculate Aspergillus niger into the enzymolysis solution, control the temperature at 32 °C, keep the humidity at 70%, and carry out fermentation for 72 hours to obtain a fermentation broth; Use an ethanol solution with a mass fraction of 70% to carry out ultrasonic-assisted extraction on the fermentation broth at 50 °C, extract 3 times in total, and the extraction time for each time is 30 minutes; After the extraction is completed, combine the extraction solutions of the 3 times to obtain a composite solution; Let the composite solution pass through a D101 macroporous resin column for chromatography, and use an ethanol solution with a mass fraction of 50% as an eluent for elution; Collect the eluate and carry out freeze-drying treatment to obtain fermented Cistanche deserticola.

[0087] Two-phase extraction: Add 10 times the mass of water to the pretreated medicinal materials, reflux and extract at 100 °C for 3 times, 40 minutes each time, combine the water extraction solution and concentrate it to a relative density of 1.0. Add an ethanol solution with a mass fraction of 90% to make the ethanol content in the mixed system reach 68%, stir for 30 minutes, stand for 24 hours, centrifuge and separate at a speed of 8000 r / min, take the supernatant, add fermented Cistanche deserticola to the supernatant, and mix evenly to obtain a composite supernatant.

[0088] Microencapsulation treatment: Mix β-cyclodextrin and stevioside evenly according to a mass ratio of 10:1.2 to obtain a preliminary mixture. Mix the preliminary mixture and the composite supernatant according to a mass ratio of 1:6 to obtain a β-cyclodextrin inclusion complex, and perform secondary coating with ethyl cellulose with an addition amount of 70% of the mass of β-cyclodextrin, and carry out spray drying under the conditions of an inlet air temperature of 175 °C, an outlet air temperature of 80 °C, a feeding speed of 25 ml / min, and an atomization pressure of 0.18 MPa to form microcapsules.

[0089] Aging process: Mix 160 parts of wine base with microcapsules, add 0.8 wt% of xanthan gum as a suspending agent, ultrasonically disperse at a frequency of 35 kHz for 10 minutes, store in a constant temperature cellar at 18 °C for 6 months, and fill nitrogen to replace oxygen every month during this period, and take out to obtain Cistanche deserticola and Cornus officinalis wine.

[0090] Comparative example 1:

[0091] On the basis of Example 1, no microencapsulation treatment is carried out, and the rest of the technical solutions are the same as those in Example 1.

[0092] Comparative example 2:

[0093] Based on Example 1, no secondary coating treatment is carried out, and the other technical solutions are the same as those in Example 1.

[0094] Comparative Example 3:

[0095] Based on Example 1, the cistanche deserticola is not fermented, and it is processed together with other raw materials. The other technical solutions are the same as those in Example 1.

[0096] Test:

[0097] Performance test

[0098] Test Example 1

[0099] The cistanche deserticola and cornel wine obtained in the examples was subjected to sensory evaluation.

[0100] Among them, the evaluation panel consisted of 10 professional wine tasters, who evaluated the samples of the examples and comparative examples from four aspects: color, aroma, taste, and aftertaste, with a full score of 100 points.

[0101] Color: Evaluate the clarity of the wine liquid, the vividness and uniformity of the color;

[0102] Aroma: Evaluate the richness, coordination, and uniqueness of the wine aroma;

[0103] Taste: Evaluate the mellow feeling, softness, and pungency of the wine liquid;

[0104] Aftertaste: Evaluate the persistence and comfort of the aftertaste left in the mouth after swallowing the wine.

[0105] The evaluation results are as follows:

[0106] Table 1

[0107] Color Score Aroma Score Taste Score Aftertaste Score Example 1 86 93 88 93 Example 2 88 91 85 92 Example 3 86 92 87 93 Comparative Example 1 93 70 78 81 Comparative Example 2 86 79 82 86 Comparative Example 3 85 89 84 90

[0108] As can be seen from Table 1, the cistanche deserticola and cornel wine prepared by the present invention has good sensory evaluation in all aspects.

[0109] Test Example 2

[0110] To test the stability of the cistanche deserticola and cornel wine, the wine samples obtained from the examples and comparative examples were placed at room temperature (25°C) for 6 months, and then the appearance, aroma, and taste of the wine samples of the examples and comparative examples were compared to see if there were obvious changes:

[0111] The results are shown as follows:

[0112] Table 2

[0113]

[0114] As can be seen from Table 2: The cistanche deserticola and cornel wine prepared by the present invention still has good stability after being placed at room temperature for 6 months.

[0115] Calculate and compare the extraction rates of active ingredients in the sample wines of the examples and the control group, and compare the differences between the examples and the control group:

[0116] Mainly focus on the extraction rates of cistanoside in Cistanche deserticola, loganin in Cornus officinalis, and vitamin C in jujube;

[0117] Control group:

[0118] Soak 6 parts of Cistanche deserticola, 6 parts of Cornus officinalis, and 4 parts of jujube directly in 155 parts of 42-degree sorghum wine for 6 months.

[0119] After the soaking is completed, take the liquor and use a high-performance liquid chromatograph to quantitatively detect the same main active ingredients.

[0120] Table 3

[0121]

[0122] As can be seen from Table 3, the cistanche and cornus wine prepared by the present invention contains a higher content of active ingredients, which is of great benefit to the physical health of men.

[0123] Antioxidant test:

[0124] In this experiment, the ABTS radical cation scavenging ability assay method was used, referring to the national standard GB / T39100-2020 "Determination of antioxidant properties of polypeptides by DPPH and ABTS methods";

[0125] Take 20 μL of the diluted sample wines of the examples and the comparative examples and add them to 2 mL of the ABTS radical cation working solution, and mix quickly.

[0126] After reacting at room temperature for 6 minutes, measure the absorbance value with a visible spectrophotometer at a wavelength of 734 nm.

[0127] Use absolute ethanol as the blank control and Trolox as the positive control to calculate the scavenging rate of each wine sample for ABTS radical cations;

[0128] Table 4

[0129]

[0130]

[0131] As can be seen from Table 4, the cistanche and cornus wine prepared by the present invention has a high antioxidant function.

[0132] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification.

Claims

1. A cistanche and cornel wine suitable for men to drink, characterized in that, It is made from the following raw materials in parts by weight: Cistanche deserticola 5 - 8 parts, Cornus officinalis 5 - 8 parts, red dates 3 - 5 parts, wine base 150 - 160 parts; The wine base is prepared from 42 - degree sorghum wine; The parameters of the sorghum wine: total acid calculated as acetic acid: 0.46 g / L; Total esters calculated as ethyl acetate: 1.51 g / L; Solids: ≤1.0 g / L; Methanol content: ≤0.6 g / L.

2. The preparation process of a Cistanche and Macrocarpium officinale wine suitable for men to drink according to claim 1, characterized in that, It includes the following steps: (1) Medicinal material treatment: First, soak Cornus officinalis and red dates in 50% ethanol by volume for 12 - 14 hours, then filter and crush to obtain crushed material; Perform supercritical carbon dioxide extraction on the obtained crushed material to remove fat - soluble impurities and obtain pretreated medicinal materials; Ferment Cistanche deserticola to obtain fermented Cistanche deserticola; (2) Biphasic extraction: Perform biphasic extraction on the above - obtained pretreated medicinal materials. Specifically, add 8 - 10 times the mass of water to the pretreated medicinal materials, perform reflux extraction at 100°C for 3 times, each time for 40 min, combine the 3 - time water extracts, concentrate to a relative density of 1.0, then add 90% ethanol solution by mass, keep the ethanol content in the mixed system at 65 - 68%, stir for 30 min, stand for 22 - 24 hours, then centrifuge and separate, take the supernatant, add fermented Cistanche deserticola to the supernatant, mix evenly to obtain a composite supernatant; (3) Microcapsule embedding treatment: First, mix β - cyclodextrin and stevioside evenly according to a mass ratio of 10:0.8 - 1.2 to obtain a preliminary mixture; Mix the preliminary mixture with the above - obtained composite supernatant according to a mass ratio of 1:5 - 6 to obtain a β - cyclodextrin inclusion complex, then perform secondary coating with ethyl cellulose, the addition amount of ethyl cellulose is 70% of the mass of β - cyclodextrin, and then perform spray drying to form a microcapsule with a core - shell structure; (4) Aging process: Mix the wine base with the above microcapsules, then add 0.05 - 0.8 wt% xanthan gum as a suspending agent, perform ultrasonic dispersion for 10 min, finally store in a constant - temperature cellar for 6 months, and then take it out.

3. The preparation process of a cistanche and dogwood wine suitable for men to drink according to claim 2, characterized in that, The soaking temperature in step (1) is 55 - 60°C; The preparation method of fermented cistanche deserticola is as follows: Clean cistanche deserticola with clear water; Cut the washed cistanche deserticola into slices with a thickness of 1 mm, and place them in an oven at 60 °C to dry until the moisture content is lower than 10%; Use a pulverizer to pulverize the dried cistanche deserticola to 80 meshes to obtain cistanche deserticola powder; Add water 10 times the mass of the cistanche deserticola powder to the cistanche deserticola powder, then add 0.5 wt% of cellulase, mix evenly, and then place the mixture in a water bath environment at 42 °C and continuously enzymolyze for 4 hours to obtain an enzymolysis solution; Inoculate Aspergillus niger into the enzymolysis solution, control the temperature at 30 - 32 °C, keep the humidity at 65 - 70%, and carry out fermentation for 72 hours to obtain a fermentation broth; Use an ethanol solution with a mass fraction of 70% to carry out ultrasonic-assisted extraction on the fermentation broth at 50 °C for a total of 3 times, with each extraction duration of 30 minutes; After the extraction is completed, combine the extraction solutions of the 3 times to obtain a composite solution; Let the composite solution pass through a D101 macroporous resin column for chromatography, and use an ethanol solution with a mass fraction of 50% as an eluent for elution; Collect the eluate and carry out freeze-drying treatment to obtain fermented cistanche deserticola.

4. The preparation process of a Cistanche and Macrocarpium officinale wine suitable for men to drink according to claim 2, characterized in that, The parameters of supercritical carbon dioxide extraction in step (1) are: The extraction pressure is 22 - 23 MPa; The extraction temperature is 45 - 48 °C.

5. The preparation process of a cistanche and dogwood wine suitable for men to drink according to claim 2, characterized in that, The rotation speed of centrifugal separation in step (2) is 8000 r / min.

6. The preparation process of a cistanche and dogwood wine suitable for men to drink according to claim 2, characterized in that, The spray-drying parameters in step (3) are: The inlet air temperature is 175 °C, the outlet air temperature is 80 °C, the feeding speed is 20 - 25 ml / min, and the atomization pressure is 0.15 - 0.18 MPa.

7. The preparation process of a cistanche and dogwood wine suitable for men to drink according to claim 2, characterized in that, The frequency of ultrasonic dispersion in step (4) is 30 - 35 kHz.

8. The preparation process of a cistanche and dogwood wine suitable for men to drink according to claim 2, characterized in that, During the cellar storage in step (4), nitrogen is filled monthly to displace oxygen.

9. The preparation process of a cistanche and cornel wine suitable for men to drink according to claim 2, characterized in that, The temperature of constant-temperature cellar storage in step (4) is 15 - 18 °C.

10. The preparation process of a cistanche and cornel wine suitable for men to drink according to claim 9, characterized in that, The temperature of constant-temperature cellar storage in step (4) is 16 °C.

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