Device for continuously culturing fresh water microalgae indoors at constant temperature

Abstract

The invention provides a device for indoor constant-temperature continuous culture of freshwater microalgae. According to the device, a cross beam and a hanging frame are installed at the top of each main frame, breeding bags are correspondingly hung on the two sides of each hanging frame, a pH value detector and an air inlet pipe are arranged in each breeding bag, a monitoring alarm box is installed on the main frame at the end, and an upper light bar and a lower light bar are installed on the two sides of each main frame and correspond to each breeding bag. Sewage discharge grooves are formed in the ground at the two outer ends of the main frame, the bottom of each culture bag is communicated with a liquid conveying mechanism, and an algae liquid conveying mechanism is arranged on the ground at the inner end of the main frame. The buffer tank is provided with a non-contact liquid level monitor, a material pipe, a material pipe electromagnetic valve and a buffer tank electromagnetic valve; and the main conveying pipe is provided with a segmented electromagnetic valve, a connecting straight pipe and a branch pipe electromagnetic valve. According to the device, the culture bags are connected in series to culture the fresh water microalgae, the liquid conveying mechanism is used for unified conveying, the structure is simple and practical, the automation degree is high, the equipment investment cost is low, and the yield of the fresh water microalgae is high.

Description

Technical Field

[0001] The present invention relates to the technical field of microalgae cultivation equipment, and particularly relates to a device for indoor constant-temperature continuous cultivation of freshwater microalgae. Background Art

[0002] Microalgae are a class of tiny single-cell algae plants that usually exist in a single-cell or multi-cell form and grow in water. The cultivation of microalgae is an emerging agricultural breeding model. It has many advantages such as environmental protection, high efficiency and sustainability, so it has attracted much attention. Its main characteristics are rapid growth, rich in nutrients such as protein, fat, carbohydrates and multiple vitamins, and are widely used in food, feed, cosmetics and medicine. The cultivation of freshwater microalgae is based on water. The growth of freshwater microalgae requires comprehensive consideration of multiple factors such as light, temperature, nutrients, pH and breeding system. Different species and environments require targeted optimization of conditions. Most freshwater microalgae grow best at around 24-26℃. Too low or too high temperatures will have an adverse effect on the growth of freshwater microalgae. Therefore, in the process of cultivating freshwater microalgae, it is necessary to control the temperature and make appropriate adjustments. Freshwater microalgae are sensitive to pH values. During the cultivation process, it is necessary to maintain an appropriate pH value. Too high or too low pH values will have an adverse effect on the growth of microalgae. The optimal growth pH range of freshwater microalgae is 7.0-8.5. During artificial cultivation, the pH value may fluctuate due to nutrient absorption, metabolism or changes in carbon dioxide concentration. It is necessary to maintain stability by adjusting the amount of mixed gas (the mixed gas contains 1%-5% carbon dioxide) or the composition of the nutrient solution. Freshwater microalgae have high requirements for light. Generally speaking, strong light is conducive to the photosynthesis and growth and development of freshwater microalgae. There is a certain relationship between the density and light intensity of freshwater microalgae. Too high aquaculture density or too low a light intensity will lead to competition and insufficient photosynthesis of freshwater microalgae. Therefore, when cultivating microalgae, it is necessary to control the cultivation density and light intensity to ensure the normal growth of microalgae. When cultivating freshwater microalgae, it is necessary to choose an environment with moderate light intensity and make appropriate adjustments. The illumination time is generally 12-16 hours, and the light source can be sunlight or artificial light. Freshwater microalgae require sufficient nutrients to grow normally. Common nutrients include nitrogen, phosphorus, potassium, etc., which can be provided by adding appropriate amounts of compound fertilizers or other special fertilizers. In addition, some trace elements and organic matter can be added to promote the growth of freshwater microalgae. Freshwater microalgae are susceptible to pollution and pests and diseases. Therefore, during the breeding process, it is necessary to pay attention to maintaining cleanliness and hygiene to prevent exogenous pollution and the invasion of pests and diseases. The breeding equipment, delivery pipelines and filtered culture medium can be cleaned regularly, and disinfection work should be strengthened. In short, freshwater microalgae breeding is a potential breeding model, but it is necessary to pay attention to light, temperature, nutrient supply, water quality monitoring, etc. to maintain a stable growth environment; at the same time, it is necessary to prevent disease infection and the occurrence of pests and diseases, regularly clean equipment, disinfect water bodies, and keep the breeding equipment clean.

[0003] Currently, most of the artificial cultivation of freshwater microalgae in the prior art is carried out outdoors using closed glass pipelines, which requires suitable temperatures. For example, Haematococcus pluvialis is suitable for growth at around 25 degrees Celsius. In Yunnan, the temperature difference throughout the year is not significant, and the temperature is around 25 degrees Celsius for most of the time. Therefore, Haematococcus pluvialis is cultivated outdoors in some areas of Yunnan using closed glass pipelines. Since the outdoor temperature cannot be completely kept constant, the water cycle is also used to regulate the temperature in Yunnan. The closed glass pipelines can effectively control the environment, but the pipeline laying is relatively complex, the floor area of the pipelines is large, and a large number of various pumps need to be invested. Therefore, the equipment investment cost and operation cost of this cultivation method are both relatively high.

[0004] Another outdoor cultivation method for freshwater microalgae is to build open cultivation ponds on the ground, add a culture medium to the cultivation ponds for cultivation, and the microalgae cultivated in the cultivation ponds also need temperature control. Although the cost of building the cultivation ponds is relatively low, the external environment of the cultivation ponds cannot be artificially controlled. At the same time, the microalgae cultivated in the cultivation ponds are also easily infected with miscellaneous bacteria and protozoa, which is highly harmful to the growth of microalgae, and the yield of the microalgae cultivated in the cultivation ponds is not high.

[0005] Another indoor cultivation method for freshwater microalgae is to hang and cultivate using transparent plastic bags, and the algal liquid is transported into the transparent plastic bags for cultivation. Although the equipment investment cost of this cultivation method is relatively low and the cultivation environment can be effectively controlled, the cultivation bags are independent of each other and are only suitable for small-scale cultivation of freshwater microalgae. Generally, this cultivation method is only used for cultivating algal seeds.

[0006] In summary, to solve the problems existing in the prior art, the technical personnel of this application have designed a device for indoor constant-temperature continuous cultivation of freshwater microalgae. Summary of the Invention

[0007] The purpose of the present invention is to provide a device for indoor constant-temperature continuous cultivation of freshwater microalgae. The technical problem to be solved by the present invention is: adopting the method of connecting cultivation bags in series for indoor constant-temperature cultivation, and at the same time using a buffer tank, a main delivery pipe and connecting branch pipes to uniformly deliver the algal liquid and nutrient solution into each cultivation bag, using a pH meter to monitor the pH value in the cultivation bag, and using the light emitted by a light bar to simulate sunlight irradiation on the algal liquid in the cultivation bag to solve the technical problem of large-scale indoor cultivation of freshwater microalgae.

[0008] The design concept of the present invention is as follows: In the breeding workshop, two columns and N rows of main frames are arranged side by side according to the floor area of the workshop. There are walkways left between the two columns of main frames and each row of main frames. Cross beams and hanging racks are installed on each row of main frames. Upper light strips and lower light strips are respectively installed on both sides of the main frames. The breeding bags are correspondingly suspended on the hanging racks. The bottom of each breeding bag is connected to the main delivery pipe through a connecting branch pipe and is controlled by a branch pipe solenoid valve. A sectional solenoid valve is installed on the main delivery pipe to control the sectional breeding of the algal liquid. A buffer tank is arranged at one end of the main frame. When the buffer tank is installed, the height of its lower part should be higher than the bottom of the breeding bag, forming a certain height difference between the buffer tank and the breeding bag. The main delivery pipe is connected to the buffer tank. A buffer tank solenoid valve is installed at the bottom of the buffer tank to control the delivery of the algal liquid or nutrient solution into the main delivery pipe. A material pipe for inputting various liquid materials is installed at the top of the buffer tank, and each material pipe is respectively controlled by a material pipe solenoid valve. Various liquid materials can be input into the buffer tank in proportion in sequence for mixing, and then be delivered into the main delivery pipe through the buffer tank solenoid valve. By using the height difference formed between the buffer tank and the breeding bag, the algal liquid or nutrient solution is delivered into each breeding bag under the action of gravity. A monitoring and alarm box is installed at the other end of the main frame. A pH meter is arranged in the first breeding bag of each row, and the pH meter is connected to the monitoring and alarm box through a wire. The function of the monitoring box is to monitor the temperature change in the breeding workshop and the pH value change in the breeding bag. When the pH value in the breeding bag exceeds the set range, the monitoring and alarm box gives an audible and visual alarm. Sewage troughs are arranged on the ground at both outer ends of the main frame. The sewage from the breeding bags, buffer tank and each pipeline after cleaning and disinfection is discharged into the sewage trough through the main delivery pipe, and the discharge of the sewage is controlled by a sewage solenoid valve. The sewage is discharged into the sewage treatment system through the sewage trough for treatment. An algal liquid collection trough is arranged on the ground at the inner end of the main frame. The mature algal liquid in the breeding bag is discharged into the algal liquid collection trough through the main delivery pipe, and the discharge of the algal liquid is controlled by a discharge solenoid valve. The algal liquid in the collection trough is delivered into a collection tank, and the algal liquid is delivered to the next process for treatment through a liquid pump.

[0009] The temperature in the breeding workshop is regulated by the central air conditioner. When the monitoring and alarm box detects that the temperature in the breeding workshop is too high or too low, it will send a signal to the central control room. The operator issues an instruction to the central air conditioner, and the central air conditioner regulates the temperature in the breeding workshop. All kinds of nutrient solutions required for the cultivation of freshwater microalgae are prepared in the liquid batching room and then transported to the buffer tank through the material pipe. The mixed gas containing carbon dioxide required for the cultivation of freshwater microalgae is prepared in the gas mixing room. The preparation of the nutrient solution and the mixed gas are both controlled by the operator in the central control room. In addition, a breeding room is set up in the breeding workshop for cultivating the algal seeds of freshwater microalgae. At the same time, a personnel dressing room, a disinfection and sterilization room, and a visiting aisle are set up. When external personnel visit, they can only visit along the aisle in the visiting aisle to avoid bringing external germs into the workshop, so as to ensure the safety and hygiene in the breeding workshop and prevent the algal liquid in the breeding bag from being infected by viruses, affecting the normal growth of freshwater microalgae.

[0010] To achieve the above object, the technical solution of the invention is as follows: A device for indoor constant-temperature continuous cultivation of freshwater microalgae, comprising: a main frame, breeding bags, a pH value detector, a monitoring and alarm box, a sewage discharge solenoid valve, a sewage discharge tank, a cross beam, hanging brackets, upper lamp strips, lower lamp strips, an air inlet pipe, a visiting aisle, a breeding room, a central control room, a disinfection and sterilization room, a personnel dressing room, a central air conditioner room, a gas mixing room, a liquid conveying mechanism, and an algal liquid conveying mechanism. The liquid conveying mechanism includes: a main conveying pipe, a connecting straight pipe, branch solenoid valves, sectional solenoid valves, brackets, a buffer tank solenoid valve, a buffer tank, a material pipe solenoid valve, a material pipe, a non-contact liquid level monitor, and a liquid batching room. The algal liquid conveying mechanism includes: an algal liquid collection tank, a discharge solenoid valve, a liquid pump, and a collection tank. Cross beams are installed on the tops of the main frames, and hanging brackets are fixedly installed on the cross beams at intervals. Breeding bags are hung on both sides of each hanging bracket correspondingly. A pH value detector is arranged in the breeding bag close to the end main frame, and a monitoring and alarm box is installed on the end main frame. The pH value detector is connected to the monitoring and alarm box through a wire. An air inlet pipe is arranged on the lower side of each breeding bag, and the air inlet pipe is communicated with the output pipeline of the gas mixing room. Upper lamp strips and lower lamp strips are installed on both sides of the main frame corresponding to each breeding bag respectively. Sewage discharge tanks are arranged on the ground at both outer ends of the main frame, and a sewage discharge solenoid valve is installed on the main conveying pipe above the sewage discharge tank. The sewage discharge tank is communicated with the sewage treatment system. The bottom of each breeding bag is communicated with the liquid conveying mechanism, and an algal liquid conveying mechanism is arranged on the ground at the inner end of the main frame.

[0011] The liquid delivery mechanism in the device is provided with a buffer tank at the end of the main frame. A bracket is welded to the bottom of the buffer tank. A non-contact liquid level monitor is installed on one side of the buffer tank. A material pipe is installed on the top of the buffer tank. A material pipe solenoid valve is installed on the material pipe. Each material pipe is connected to the corresponding output pipe in the liquid batching room. A buffer tank solenoid valve is installed on the main delivery pipe at the bottom of the buffer tank. A sectional solenoid valve is installed on the horizontal section of the main delivery pipe. Connecting straight pipes are respectively installed at the positions corresponding to each breeding bag on the horizontal section of the main delivery pipe. A branch pipe solenoid valve is installed on each connecting straight pipe. Each connecting straight pipe is respectively communicated with the bottom of the corresponding breeding bag.

[0012] The algal liquid delivery mechanism in the device is provided with an algal liquid collection tank on the ground at the inner end of the main frame. An outlet solenoid valve is installed on the outlet pipe above the algal liquid collection tank. The end of the algal liquid collection tank is communicated with a collection tank. The liquid output pipe of the collection tank is communicated with the input pipe of a liquid pump. The output pipe of the liquid pump is communicated with the next process.

[0013] The installation of the main frame in the device is set according to the area of the breeding workshop. A passage for people to walk is left between each row of main frames. A viewing passage is arranged on one side of the breeding workshop. A breeding room is arranged at one end inside the breeding workshop. A disinfection and sterilization room, a central control room and a personnel dressing room are successively arranged outside the breeding room. An air-conditioning room is arranged on one side of the personnel dressing room. A gas mixing room and a liquid batching room are successively arranged on one side of the air-conditioning room. An air compressor and a carbon dioxide storage tank are installed in the gas mixing room. The mixed gas output pipe of the gas mixing room is communicated with the inlet pipe. Each liquid output pipe in the liquid batching room is respectively communicated with the corresponding material pipe.

[0014] The monitoring and alarm box, sewage discharge solenoid valve, branch pipe solenoid valve, sectional solenoid valve, outlet solenoid valve, buffer tank solenoid valve, material pipe solenoid valve, non-contact liquid level monitor, upper lamp strip, lower lamp strip, liquid pump, air-conditioning room, gas mixing room in the device are respectively connected to the electric control unit in the central control room by wires.

[0015] For the installation of the buffer tank in the device, the lower part of the tank body should be higher than the top of the breeding bag, so as to form a height difference between the buffer tank and the breeding bag. Several rows of main frames and breeding bags can share one buffer tank as needed. Each solenoid valve, liquid pump and buffer tank involved in the device are mature products in the prior art. The monitoring and alarm box selects the product produced by Hangzhou Liance Automation Technology Co., Ltd., and the non-contact liquid level monitor selects the product produced by Shenzhen Xingkechuang Technology Co., Ltd.

[0016] Compared with the prior art, the positive effects of the present invention are: 1. The device uses the method of connecting culture bags in series to cultivate freshwater microalgae at a constant temperature indoors. At the same time, a liquid delivery mechanism is used to uniformly deliver algal liquid and nutrient solution into each culture bag, solving the technical problem of large-scale indoor cultivation of freshwater microalgae. 2. The liquid delivery mechanism in the device includes a liquid batching room, a buffer tank, a main delivery pipe, and connecting branch pipes. After the nutrient solution is prepared in proportion in the liquid batching room, it is transported through pipelines to the buffer tank and mixed therein. The buffer tank uses the height difference with the culture bags to uniformly deliver the nutrient solution into each culture bag under the action of gravity. The nutrient solution can be delivered after mixing, or a certain nutrient solution can be delivered alone. 3. An air inlet pipe is provided on one side of each culture bag in the device. The air inlet pipe is connected to a gas mixing chamber. The mixed gas added with carbon dioxide prepared in the gas mixing chamber enters the culture bag through the air inlet pipe to provide the necessary carbon source for freshwater microalgae during the growth period. 4. A pH meter is provided in the first culture bag of the same growth period freshwater microalgae in each row of the device. The pH meter is used to monitor the change of the pH value in the culture bag and adjust the pH value in the culture bag by increasing or decreasing the content of carbon dioxide in the mixed gas. 5. Upper and lower light strips are installed on both sides of each main frame of the device. By turning on or off some of the light strips, the required light is provided for freshwater microalgae in different growth periods to ensure photosynthesis during the growth period of microalgae. 6. The control and adjustment of the indoor temperature of the device are completed by the operator in the central control room regulating the central air conditioner to ensure that the overall temperature of the cultivation workshop is within the temperature range suitable for the growth of freshwater microalgae. 7. The freshwater microalgae cultivated in the culture bags in the device are centrally discharged into the collection tank through an algal liquid collection tank, and a liquid pump is used to transport the algal liquid in the collection tank to the next process for treatment. 8. The sewage after disinfection and cleaning of the device is centrally discharged into the sewage treatment system through a sewage discharge tank, and the sewage treatment system centrally treats the sewage. After the sewage reaches the standard, it can be recycled or discharged. 9. The device has a simple and practical structure, a high degree of automation, and it is very simple and easy to carry out unified cultivation or segmented cultivation. The equipment investment cost is relatively low, and the harvest of freshwater microalgae is relatively high, realizing large-scale indoor constant temperature cultivation of freshwater microalgae. Description of the Drawings

[0017] For a clearer description, the drawings required for implementing the technology of the present invention are briefly introduced below. The drawings in the following description are only exemplary. For those of ordinary skill in the art, other implementation drawings can be obtained by extending according to the provided drawings without creative efforts.

[0018] The structures, proportions, sizes, etc. of the drawings shown in this specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the conditions for the implementation of the invention. Any modification of the structure, change in the proportional relationship, or adjustment of the size, without affecting the effects that can be produced by the technology of the present invention and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in the present invention.

[0019] Figure 1 , Front view structural schematic diagram of the freshwater microalgae cultivation device; Figure 2 , Side view structural schematic diagram of the freshwater microalgae cultivation device; Figure 3 , Planar structural schematic diagram of the freshwater microalgae cultivation workshop; Figure 4 , Connection block diagram of each component and the central control room.

[0020] In the figure: 1, main frame; 2, cultivation bag; 3, pH detector; 4, monitoring and alarm box; 5, sewage discharge solenoid valve; 6, sewage discharge tank; 7, main delivery pipe; 8, connecting straight pipe; 9, branch solenoid valve; 10, sectional solenoid valve; 11, algal liquid collection tank; 12, discharge solenoid valve; 13, bracket; 14, buffer tank solenoid valve; 15, buffer tank; 16, material pipe solenoid valve; 17, material pipe; 18, non-contact liquid level monitor; 19, cross beam; 20, hanging rack; 21, upper lamp strip; 22, lower lamp strip; 23, intake pipe; 24, viewing walkway; 25, liquid pump; 26, collection tank; 27, breeding room; 28, central control room; 29, disinfection and sterilization room; 30, personnel dressing room; 31, central air conditioning room; 32, liquid batching room; 33, gas mixing chamber. Specific embodiments

[0021] The technical solutions of the present invention will be further clearly and completely described below by combining the drawings and embodiments. The following specific embodiments are only used for further illustration of the present application and should not be construed as limiting the protection scope of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0022] See the appendix Figures 1-4, Installation and connection of the device. Calculate the installation quantity of the main frame 1 according to the floor area of the breeding workshop, install the main frame 1 in two columns, with N rows of main frames 1 in each column, and leave a passage for people to walk between the two columns of main frames 1 and each row of main frames 1. The cross beam 19 is installed on the top of each main frame 1 to connect each main frame 1 into a whole. The hanging frames 20 are fixedly installed on the cross beam 19 at intervals, and the breeding bags 2 are correspondingly suspended on both sides of each hanging frame 20. The bottom of each breeding bag 2 is connected to the liquid conveying mechanism. The pH value detector 3 is arranged in the breeding bag 2 close to the end main frame 1, and the monitoring and alarm box 4 is installed on the end main frame 1. The pH value detector 3 is connected to the monitoring and alarm box 4 through a wire. The air inlet pipes 23 are respectively arranged on the lower side of each breeding bag 2, and the air inlet pipes 23 are connected to the output pipeline of the gas mixing chamber 33. The upper light strip 21 and the lower light strip 22 are respectively installed on both sides of each main frame 1, and the upper light strip 21 and the lower light strip 22 respectively correspond to each breeding bag 2. The sewage discharge tank 6 is arranged on the ground at both outer ends of the main frame 1, the sewage discharge solenoid valve 5 is installed on the main conveying pipe 7 above the sewage discharge tank 6, the sewage discharge tank 6 is connected to the sewage treatment system, and the algal liquid conveying mechanism is arranged on the ground at the inner end of the main frame 1.

[0023] The buffer tank 15 is arranged at the end of the main frame 1. When installing the buffer tank 15, its lower part should be higher than the top of the breeding bag 2 to form a certain height difference between the buffer tank 15 and the breeding bag 2. The support 13 is welded at the bottom of the buffer tank 15, the non-contact liquid level monitor 18 is installed on one side of the buffer tank 15, the material pipe 17 is installed on the top of the buffer tank 15, the material pipe solenoid valve 16 is installed on the material pipe 17, and each material pipe 17 is respectively connected to the output pipe corresponding to the liquid batching room 32. The buffer tank solenoid valve 14 is installed on the main conveying pipe 7 at the bottom of the buffer tank 15, the sectional solenoid valve 10 is installed on the horizontal section of the main conveying pipe 7, the connecting straight pipes 8 are respectively installed at the positions corresponding to each breeding bag 2 on the horizontal section of the main conveying pipe 7, the branch solenoid valves 9 are respectively installed on each connecting straight pipe 8, and each connecting straight pipe 8 is respectively connected to the bottom of the corresponding breeding bag 2.

[0024] The algal liquid collection tank 11 is arranged on the ground at the inner end of the main frame 1, the discharge solenoid valve 12 is installed on the discharge pipe above the algal liquid collection tank 11, the end of the algal liquid collection tank 11 is connected to the collection tank 26, the liquid output pipe of the collection tank 26 is connected to the input pipe of the liquid pump 25, and the output pipe of the liquid pump 25 is connected to the next process.

[0025] The visiting aisle 24 is arranged on one side of the breeding workshop, the breeding room 27 is arranged at one end inside the breeding workshop, the central control room 28 is arranged outside the partition wall of the breeding room 27, the disinfection and sterilization room 29 and the personnel dressing room 30 are successively arranged on both sides of the central control room 28, the central air-conditioning room 31 is arranged on one side of the personnel dressing room 30, the gas mixing room 33 is arranged on one side of the central air-conditioning room 31, and the liquid batching room 32 is arranged on one side of the gas mixing room 33. The air compressor and the carbon dioxide storage tank are installed in the gas mixing room 33. The mixed gas output pipe of the gas mixing room 33 is communicated with the intake pipe 23, and each liquid output pipe of the liquid batching room 32 is respectively communicated with the corresponding material pipe 17.

[0026] Number the sewage solenoid valve 5, each branch solenoid valve 9, the sectional solenoid valve 10, the discharge solenoid valve 12, the buffer tank solenoid valve 14, and the material pipe solenoid valve 16 in the device, and then connect them to the electronic control unit in the central control room 28 with wires respectively. Connect the monitoring and alarm box 4, the non-contact liquid level monitor 18, the upper light bar 21, the lower light bar 22, the liquid pump 25, the central air-conditioning room 31, and the gas mixing room 33 to the electronic control unit in the central control room 28 with wires respectively. Embodiment 1

[0027] The situation of overall breeding of freshwater microalgae in the breeding workshop Calculate the installation quantity of the main frame 1 according to the floor area of the breeding workshop, and install the main frame 1 in two columns. Each column is provided with N rows of the main frame 1, and a pedestrian passage is left between the two columns of the main frame 1 and each row of the main frame 1. The cross beam 19 is installed on the top of each main frame 1 to connect each main frame 1 into a whole. The hanging frames 20 are fixedly installed on the cross beam 19 at intervals, and the breeding bags 2 are correspondingly hung on both sides of each hanging frame 20. The bottom of each breeding bag 2 is communicated with the liquid conveying mechanism. The pH value detector 3 is arranged in the breeding bag 2 close to the end main frame 1, and the monitoring and alarm box 4 is installed on the end main frame 1. The pH value detector 3 is connected with the monitoring and alarm box 4 through a wire. The air inlet pipes 23 are respectively arranged on the lower sides of each breeding bag 2, and the air inlet pipes 23 are communicated with the output pipeline of the gas mixing chamber 33. The upper lamp strip 21 and the lower lamp strip 22 are respectively installed on both sides of each main frame 1, and the upper lamp strip 21 and the lower lamp strip 22 respectively correspond to each breeding bag 2. Each material pipe 17 is respectively communicated with the output pipe corresponding to the liquid batching room 32. Number the sewage discharge solenoid valve 5, each branch solenoid valve 9, the sectional solenoid valve 10, the discharge solenoid valve 12, the buffer tank solenoid valve 14, and the material pipe solenoid valve 16, and then connect them to the electric control unit in the central control room 28 with wires respectively. Connect the monitoring and alarm box 4, the non-contact liquid level monitor 18, the upper lamp strip 21, the lower lamp strip 22, the liquid pump 25, the central air-conditioning room 31, and the gas mixing chamber 33 to the electric control unit in the central control room 28 with wires respectively. Before use, input the process parameters of the monitoring and alarm box 4 and the non-contact liquid level monitor 18, the temperature parameters of the central air conditioner in the central air-conditioning room 31, and the parameters of the carbon dioxide and air mixture in the gas mixing chamber 33 into the electric control unit in the central control room 28 respectively.

[0028] In use, first, the algal species cultivated in the breeding room 27 are transported into the buffer tank 15. Meanwhile, the operator opens the section solenoid valve 10 and the buffer tank solenoid valve 14 through the central control room 28, then opens one branch solenoid valve 9. Utilizing the height difference formed between the buffer tank 15 and the cultivation bag 2, the algal species are transported into this cultivation bag 2 by volume through the main delivery pipe 7 and the connecting straight pipe 8. Then, the branch solenoid valve 9 on the connecting straight pipe 8 is closed, and the algal species are transported into each cultivation bag 2 by volume in sequence according to this operation. After the transportation of the algal species is completed, the operator opens each branch solenoid valve 9 through the central control room 28. According to the quantity of nutrient solution required for the growth of freshwater microalgae, each nutrient solution prepared in the liquid batching room 32 is transported into the buffer tank 15 in proportion through the material pipe 17 and the material pipe solenoid valve 16, and is transported into each cultivation bag 2 through the main delivery pipe 7 and the connecting straight pipe 8 by utilizing the height difference formed between the buffer tank 15 and the cultivation bag 2. Meanwhile, the mixed gas of carbon dioxide and air in the gas mixing chamber 33 is transported into each cultivation bag 2 through the air inlet pipe 23 to provide the required carbon source for the growth of freshwater microalgae. When the non-contact liquid level monitor 18 monitors that the buffer tank 15 and the cultivation bag 2 form an equal liquid level, the operator closes the buffer tank solenoid valve 14 and each branch solenoid valve 9 through the central control room 28 to stop the transportation of the nutrient solution. According to the sunlight intensity required by the freshwater microalgae, the upper lamp strip 21 or the lower lamp strip 22 is turned on. When the freshwater microalgae need strong light irradiation for growth, the upper lamp strip 21 and the lower lamp strip 22 are turned on simultaneously to ensure the light required for the photosynthesis of the growth of freshwater microalgae.

[0029] When the pH meter 3 in the cultivation bag 2 detects that the pH value in the bag exceeds 7.0 - 8.5, the monitoring and alarm box 4 emits an audible and visual alarm sound. The operator adjusts the addition amount of carbon dioxide in the air through the central control room 28 to ensure that the pH value in the cultivation bag 2 is within the range of 7.0 - 8.5. When the temperature in the cultivation workshop is higher or lower than the set value, the monitoring and alarm box 4 also emits an audible and visual alarm sound. The operator adjusts the output temperature of the central air conditioner in the central air conditioning room 31 through the central control room 28 to ensure the temperature in the cultivation workshop.

[0030] When the cultivation of the freshwater microalgae in the cultivation bag 2 is completed, the operator opens each branch solenoid valve 9 and the discharge solenoid valve 12 through the central control room 28. The freshwater microalgae in each cultivation bag 2 are transported into the algal liquid collection tank 11 through the connecting straight pipe 8 and the main delivery pipe 7. The algal liquid enters the collection tank 26 along the algal liquid collection tank 11, and then the algal liquid is transported to the next process for treatment through the liquid pump 25.

[0031] When each cultivation bag 2, the main delivery pipe 7, the connecting straight pipe 8, and the buffer tank 15 need to be cleaned and disinfected, the disinfectant and clean water are injected in sequence to perform the disinfection and cleaning operation on the above components. The sewage discharge solenoid valve 5 is opened, and the sewage is discharged into the sewage discharge tank 6 and then into the sewage treatment system through the sewage discharge tank 6 for centralized treatment of the sewage.

[0032] When the operator enters the breeding workshop, they need to change clothes in the personnel dressing room within 30 minutes. Only after changing clothes can they enter the breeding workshop. The disinfection and sterilization room 29 is used to disinfect and sterilize the clothes, slippers and utensils changed after work. When there are external visitors, the external visitors can only visit along the visiting aisle 14 to avoid bringing external germs into the workshop and ensure the safety and hygiene in the breeding workshop. Embodiment 2

[0033] The situation of cultivating Haematococcus pluvialis in sections in the breeding workshop The main frame 1, the arrangement of the breeding bags, the pH meter 3, the setting of the monitoring and alarm box 4, the buffer tank 15, the setting of each pipeline and the installation of each solenoid valve are the same as those in Embodiment 1 and will not be repeated.

[0034] During use, first transport the algal seeds cultivated in the breeding room 27 into the buffer tank 15. At the same time, the operator opens the section solenoid valve 10 and the buffer tank solenoid valve 14 through the central control room 28, and then opens one of the branch solenoid valves 9 inside the section and closes the branch solenoid valves 9 outside the section. Utilize the height difference formed between the buffer tank 15 and the breeding bag 2 to transport the algal seeds in accordance with the quantity through the main delivery pipe 7 and the connecting straight pipe 8 into this breeding bag 2, and then close the branch solenoid valve 9 on the connecting straight pipe 8. Operate in this way to transport the algal seeds in accordance with the quantity into each breeding bag 2 inside the section in turn. The following operations of nutrient solution input, mixed gas delivery, lighting, algal liquid collection, and sewage discharge are the same as those in Embodiment 1 and will not be repeated. The breeding bags 2 outside the section are all operated in the above operation modes of algal seed input, nutrient solution input, mixed gas delivery, lighting, algal liquid collection, and sewage discharge.

[0035] Example test results Comparison table of various technical indicators for cultivating Haematococcus pluvialis using the device of the present application and cultivating Haematococcus pluvialis using the prior art.

[0036] Table 1 Various technical indicators for cultivating Haematococcus pluvialis using the prior art

[0037] Table 2 Various technical indicators for cultivating Haematococcus pluvialis using the device of the present application

[0038] It can be seen from the comparison of the technical indicators in Table 1 and Table 2 that, for the device provided by the technical solution of this application, the average cultivation cycle of Haematococcus pluvialis is shortened by 6 days, the cultivation density is doubled, the average yield is doubled, and the astaxanthin content is increased by more than 50%. The operation and control of this device are simple. When combined with artificial light sources and a central air-conditioning system, it is more controllable than natural light and the natural environment used in the prior art. Therefore, the process of cultivating Haematococcus pluvialis is controllable and the growth of Haematococcus pluvialis is stable, with a significant increase in both yield and quality. Although the power consumption increases, through the calculation of the input-output ratio, the economic benefits generated by cultivating Haematococcus pluvialis using the device provided in this application are remarkable.

[0039] The above description is only a non-limiting implementation manner of the present invention, and a large number of embodiments can be derived. For those of ordinary skill in the art, without departing from the inventive concept of the present invention and without making creative efforts, several modified and improved embodiments can be made, and all of these fall within the protection scope of the present invention.

Claims

1. An apparatus for continuously cultivating freshwater microalgae at a constant indoor temperature, comprising: Main frame (1), breeding bag (2), pH meter (3), monitoring and alarm box (4), sewage discharge solenoid valve (5), sewage discharge trough (6), cross beam (19), hanging rack (20), upper light strip (21), lower light strip (22), air inlet pipe (23), viewing walkway (24), breeding room (27), central control room (28), disinfection and sterilization room (29), personnel dressing room (30), central air conditioning room (31), gas mixing chamber (33), liquid conveying mechanism, algal liquid conveying mechanism. The liquid conveying mechanism includes: main conveying pipe (7), connecting straight pipe (8), branch solenoid valve (9), sectional solenoid valve (10), bracket (13), buffer tank solenoid valve (14), buffer tank (15), material pipe solenoid valve (16), material pipe (17), non-contact liquid level monitor (18), liquid batching room (32). The algal liquid conveying mechanism includes: algal liquid collection tank (11), discharge solenoid valve (12), liquid pump (25), collection tank (26). It is characterized in that: cross beams (19) are installed on the tops of the main frames (1), hanging racks (20) are fixedly installed on the cross beams (19) at intervals, breeding bags (2) are hung on both sides of each hanging rack (20) correspondingly, a pH meter (3) is arranged in the breeding bag (2) close to the end main frame (1), a monitoring and alarm box (4) is installed on the end main frame (1), the pH meter (3) is connected to the monitoring and alarm box (4) through a wire, air inlet pipes (23) are respectively arranged on the lower sides of each breeding bag (2), and the air inlet pipes (23) are communicated with the output pipeline of the gas mixing chamber (33). Upper light strips (21) and lower light strips (22) are installed on both sides of each main frame (1) corresponding to each breeding bag (2). Sewage discharge troughs (6) are arranged on the ground at both outer ends of the main frame (1), a sewage discharge solenoid valve (5) is installed on the main conveying pipe (7) above the sewage discharge trough (6), the sewage discharge trough (6) is communicated with the sewage treatment system, the bottom of each breeding bag (2) is communicated with the liquid conveying mechanism, and the algal liquid conveying mechanism is arranged on the ground at the inner end of the main frame (1).

2. The device for continuously cultivating freshwater microalgae at a constant indoor temperature according to claim 1, characterized in that: In the liquid conveying mechanism of the device, a buffer tank (15) is arranged at the end of the main frame (1), a bracket (13) is welded at the bottom of the buffer tank (15), a non-contact liquid level monitor (18) is installed on one side of the buffer tank (15), a material pipe (17) is installed on the top of the buffer tank (15), a material pipe solenoid valve (16) is installed on the material pipe (17), each material pipe (17) is respectively connected to the output pipe corresponding to the liquid batching room (32), a buffer tank solenoid valve (14) is installed on the main conveying pipe (7) at the bottom of the buffer tank (15), a sectional solenoid valve (10) is installed on the horizontal section of the main conveying pipe (7), connecting straight pipes (8) are respectively installed on the horizontal section of the main conveying pipe (7) corresponding to the position of each breeding bag (2), a branch solenoid valve (9) is installed on each connecting straight pipe (8), and each connecting straight pipe (8) is respectively connected to the bottom of the corresponding breeding bag (2).

3. The device for continuously culturing freshwater microalgae at a constant indoor temperature according to claim 1, wherein: In the device, an algal liquid collection tank (11) is arranged on the ground at the inner end of the main frame (1). An outlet solenoid valve (12) is installed on the outlet pipe above the algal liquid collection tank (11). The end of the algal liquid collection tank (11) is communicated with a collection tank (26). The liquid output pipe of the collection tank (26) is communicated with the input pipe of a liquid pump (25). The output pipe of the liquid pump (25) is communicated with the next process.

4. The device for continuously culturing freshwater microalgae at a constant indoor temperature according to claim 1, wherein: The installation of the main frame (1) in the device is set according to the area of the breeding workshop. A passage for people to walk is left between each row of the main frames (1). A viewing passage (24) is arranged on one side of the breeding workshop. A breeding room (27) is arranged at one end inside the breeding workshop. A disinfection and sterilization room (29), a central control room (28), and a personnel dressing room (30) are successively arranged outside the breeding room (27). An air-conditioning central control room (31) is arranged on one side of the personnel dressing room (30). A gas mixing room (33) and a liquid batching room (32) are successively arranged on one side of the air-conditioning central control room (31). An air compressor and a carbon dioxide storage tank are installed in the gas mixing room (33). The mixed gas output pipe of the gas mixing room (33) is communicated with an inlet pipe (23). The liquid output pipes of the liquid batching room (32) are respectively communicated with the corresponding material pipes (17).

5. The device for continuously cultivating freshwater microalgae at a constant indoor temperature according to claim 1, characterized in that: The monitoring and alarm box (4), sewage solenoid valve (5), branch solenoid valve (9), sectional solenoid valve (10), outlet solenoid valve (12), buffer tank solenoid valve (14), material pipe solenoid valve (16), non-contact liquid level monitor (18), upper light bar (21), lower light bar (22), liquid pump (25), air-conditioning central control room (31), and gas mixing room (33) in the device are respectively connected to the electric control unit in the central control room (28) by wires.

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