New coriolus versicolor strain and fungus grass cultivation method thereof

Abstract

The invention relates to a novel coriolus versicolor strain and a cultivation method thereof. The new coriolus versicolor strain is coriolus versicolor TvJC209, is obtained through tissue isolation and purification, ultraviolet mutagenesis and directional screening, is preserved in the China Center for Type Culture Collection on June 19, 2023, has the preservation number of CCTCC NO: M 20231064, and is preserved in Wuhan University, Wuhan, China. The ITS sequence of the strain and the Trametes versicola voucher ACAD21091F (OL741530.1) with the highest similarity in an NCBI (National Center of Biotechnology Information) database have a difference of one basic group, namely A-G; the ITS sequence of the strain and the Trametes versicola voucher ACAD21091F (OL741530.1) have the highest similarity in the NCBI database; the strain can be cultivated by taking various dry or fresh pennisetum sinese, bamboo reed and other pennisetum sinese as main raw materials of a culture medium; the polysaccharide content of the strain seed entity reaches 6%, the water-soluble extract content reaches 22%, the triterpene content reaches 0.42%, and the biological conversion rate reaches 20%. The cultivation method of the strain comprises the steps of mother strain preparation, stock seed preparation, cultivation and ganoderma lucidum emergence management. The method has the advantages that the fungus grass which is a green, organic and sustainable raw material can be used for cultivating the coriolus versicolor, and high-quality coriolus versicolor is provided.

Description

Technical Field

[0001] The invention belongs to the field of microbial cultivation, and particularly relates to a new strain of Coriolus versicolor and a cultivation method thereof. Background Art

[0002] Trametes versicolor belongs to Fungi, Basidiomycetes, Hymenomycetes, Homobasidiomycetes, Polyporaceae, and Trametes, and is now included in the Chinese Pharmacopoeia. As a precious medicinal fungus, Trametes versicolor has the effects of protecting the liver, anti-inflammatory, anti-tumor, anti-oxidation, and enhancing immunity. It is a nourishing and strong treasure. The unique medicinal value of Trametes versicolor has become a hot spot for research and development by scholars at home and abroad and the medical industry, with good economic value and broad application prospects. The fruiting bodies of wild Trametes versicolor are single or continuous, and a single fruiting body is fan-shaped, semicircular or shell-shaped, sessile, with thin edges and disappearance of white edges when mature, flat or curled into waves. The cap is leathery, and the surface of the mature cap is mostly yellow-brown, gray-brown, gray-yellow, etc., densely covered with villi of varying lengths, with narrow, multi-colored, alternately arranged cloud-like concentric rings; there are multiple white holes on the ventral surface. However, almost all the Yunzhi medicinal materials currently used in the market are collected from the wild. There are many problems such as unstable yield, quality that does not meet the pharmacopoeia standards, and poor agronomic traits. As a result, the output of wild Yunzhi cannot meet market demand, seriously affecting the development of the Yunzhi industry. Therefore, we want to collect wild Yunzhi for separation and purification, ultraviolet mutagenesis, targeted screening, identification and domestication and cultivation, so as to protect the germplasm resources and provide certain support for the large-scale cultivation and development of Yunzhi.

[0003] If Yunzhi is cultivated with logs and sawdust, a large amount of wood will be consumed, forming a "mushroom-forest contradiction". Cottonseed hulls, as one of the main raw materials for cultivating edible and medicinal fungi, generally have problems of excessive pesticide residues or excessive heavy metals, which do not meet the requirements of green food. Therefore, it is necessary to explore other raw materials that can replace sawdust, cottonseed hulls and other raw materials for cultivating Yunzhi.

[0004] Juncao refers to herbaceous plants that contain nutrients suitable for the growth of microorganisms such as edible fungi and medicinal fungi and have comprehensive development and utilization value. Using herbaceous plants instead of wood to cultivate edible and medicinal fungi can not only achieve sustainable development of the fungi industry, but also reduce the consumption of wood and protect the ecological environment. Experiments have shown that edible and medicinal fungi cultivated with Juncao as raw material have higher yields and nutritional value than traditional raw materials such as sawdust and cottonseed hulls, and the heavy metal content of edible and medicinal fungi cultivated with Juncao is lower than that of traditional sawdust formula.

[0005] The present invention relates to a basidiocarp that is annual, leathery to semi-fibrous, sessile laterally, often imbricate and overlapping, with the pileus semi-circular to shell-shaped; the pileus surface is white when young and gradually turns dark, with dense fine villi of varying lengths, showing various colors such as gray, white, brown, blue, purple, black, etc., forming cloud-like concentric ring patterns; the pileus margin is thin and sharp, wavy and pale. The pore surface is white initially and gradually turns yellowish-brown, reddish-brown to light grayish-black; the isolation, purification, identification and cultivation of a new strain of Trametes versicolor suitable for cultivation with mushroom grass are provided. The strain is Trametes versicolor TvJC209, deposited in the China Center for Type Culture Collection, with the deposit number CCTCC NO: M 20231064, and the GenBank number of the characteristic sequence in NCBI is OR058882. The artificial cultivation method of Trametes versicolor TvJC209 includes the preparation of mother spawn, the preparation of original spawn, cultivation and fruiting body management. With this strain and the method and formula for artificial cultivation of Trametes versicolor, high-quality Trametes versicolor can be produced using green, organic and sustainable mushroom grass as raw materials, with high economic and ecological value.

[0006] There is no patent found yet on cultivating Trametes versicolor with mushroom grass. Summary of the Invention

[0007] The purpose of the present invention is to provide a new strain of Trametes versicolor and its cultivation method.

[0008] To achieve the above purpose, the present invention adopts the following technical solutions: In the first aspect of the present invention, Trametes versicolor TvJC209 is provided, classified as Trametes versicolor, and was deposited in the China Center for Type Culture Collection on June 19, 2023, with the deposit number CCTCC NO: M 20231064, and the deposit address is Wuhan University, China.

[0009] In the second aspect of the present invention, a cultivation method of Trametes versicolor TvJC209 with mushroom grass is provided, which includes the following steps: S1: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing the mother spawn medium, and incubate it in the dark in an incubator at 25 - 30 °C until the mycelium fills the test tube to obtain the mother spawn; S2: Inoculate the mother spawn into a plastic bag containing the solid original spawn medium, and incubate it in the dark in an incubator at 25 - 30 °C until the mycelium fills the plastic bag to obtain the original spawn; or inoculate the mother spawn into a shaking flask containing the liquid original spawn medium, and incubate it in the dark on a shaker at a temperature of 25 - 30 °C and a rotation speed of 120 - 160 rpm until mycelial pellets appear to obtain the original spawn; S3: Inoculate the original strain into the fungus bags containing the cultivation medium, and conduct dark cultivation in a culture room at a temperature of 25-30°C and a relative air humidity of 65%-75% until the mycelium fills the fungus bags; transfer the fungus bags with mature mycelium development into the mushroom shed for fruiting management. During fruiting management, the mycelium is fully in contact with the air, the temperature in the shed is 25-28°C, the relative air humidity is 80%-90%, and scattered light and sufficient oxygen are provided in the shed, and cultivate until the fruiting bodies are mature.

[0010] Further, the formula of the above mother strain medium is calculated by mass percentage as follows: potato flour 3%-7%, glucose 1%-2.5%, brown sugar 0.5%-1%, agar powder 1.5%-3%, defatted and desalted fish meal 0.2%-0.5%, feed-grade yeast powder 0.1%-0.4%, ferrous sulfate heptahydrate 0.001%-0.01%, magnesium sulfate 0.1%-0.2%, calcium sulfate heptahydrate 0.2%-0.5%, sodium chloride 0.1%-0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1%-0.2%, and the balance is water.

[0011] Further, the formula of the above solid original strain medium is: wheat 5%-15%, sorghum 5%-15%, corncob 15%-30%, wheat bran 5%-10%, brown sugar 0.1%-0.5%, fish meal 0.1%-0.5%, gypsum 0.5%-1.5%, calcium oxide 0.5%-1.5%, magnesium oxide 0.2%-0.5%, ferrous sulfate heptahydrate 0.002%-0.02%, magnesium sulfate 0.1%-0.2%, sodium chloride 0.1%-0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1%-0.2%, and the mushroom grass makes up the balance; adjust the water content to 55%-65%; Among them, the mushroom grass is any one or more of Arundo donax, Pennisetum giganteum, Neyraudia reynaudiana, Phragmites australis, Cymbopogon citratus, Themeda villosa, Miscanthus sacchariflorus, Miscanthus floridulus, Sorghum propinquum, Pennisetum purpureum, Dicranopteris dichotoma; calculate according to the water content of the fresh mushroom grass, and according to the principle of equal dry basis weight, the dry mushroom grass can be replaced by the fresh mushroom grass.

[0012] Further, the formula of the above liquid original strain medium is calculated by mass percentage as follows: potato flour 1%-3%, glucose 1%-3%, brown sugar 0.5%-1%, peptone 0.1%-0.6%, yeast extract powder 0.1%-0.4%, ferrous sulfate heptahydrate 0.001%-0.01%, magnesium sulfate 0.1%-0.2%, calcium sulfate heptahydrate 0.1%-0.2%, sodium chloride 0.1%-0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1%-0.2%, and the balance is water.

[0013] Further, the formula of the above cultivation material is as follows by mass percentage: wheat bran or rice bran 10% - 20%, gypsum 0.5% - 2%, calcium oxide 0.5% - 1.5%, magnesium oxide 0.1% - 0.2%, ferrous sulfate heptahydrate 0.002% - 0.02%, magnesium sulfate 0.1% - 0.2%, sodium chloride 0.1% - 0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1% - 0.2%, and the remaining amount is made up by Juncao; adjust the water content to 55% - 65%; Among them, Juncao is any one or more of Arundo donax, Pennisetum giganteum, Neyraudia reynaudiana, Phragmites australis, Cymbopogon citratus, Themeda villosa, Miscanthus sacchariflorus, Miscanthus floridulus, Sorghum propinquum, Pennisetum purpureum, Dicranopteris dichotoma; calculated according to the water content of fresh Juncao, and in accordance with the principle of equal dry weight, dry Juncao can be replaced by fresh Juncao.

[0014] The remarkable advantages of the present invention are as follows: 1) Using Juncao, a green, organic and sustainable raw material, to cultivate Trametes versicolor Trametes versicolor is a wood saprophytic fungus. A large amount of traditional materials such as sawdust and cottonseed hulls are used in the cultivation technology. However, raw materials such as sawdust and cottonseed hulls generally have excessive pesticide residues or heavy metal exceedance, which do not meet the requirements of green food, restricting the development of the Trametes versicolor industry. Moreover, Trametes versicolor cultivated with Juncao has higher contents of amino acids, polysaccharides, triterpenoids, flavonoids, ergosterol and other active substances than Trametes versicolor cultivated with sawdust.

[0015] 2) Fresh Juncao can be used to replace part of the culture material to cultivate Trametes versicolor Currently, dried raw materials are basically used in the cultivation of edible and medicinal fungi, which requires time, space and personnel, and also requires a warehouse to store the dried raw materials. Using fresh Juncao for cultivation can eliminate the drying step, reduce costs, and can be used in regions and seasons where it is inconvenient to dry the raw materials.

[0016] 3) Producing high-quality Trametes versicolor. Almost all of the Trametes versicolor medicinal materials used in the current market come from wild collection, and there are problems such as failing to meet the pharmacopoeia standards and poor agronomic characters. In the Pharmacopoeia of the People's Republic of China, it is stipulated that the polysaccharide content of the fruiting bodies of Trametes versicolor shall not be less than 3.2%, and the water-soluble extract content shall not be less than 18%. For Trametes versicolor TvJC209 of the present invention cultivated under Juncao conditions, the polysaccharide content of the fruiting bodies can reach 6%, the water-soluble extract content can reach 22%, the triterpenoid content can reach 0.42%, and the biological conversion rate can reach 20%. Description of the Drawings

[0017] Figure 1 : Mycelial plate diagram of Trametes versicolor TvJC209.

[0018] Figure 2 : Microscopic structure diagram of the mycelium of Trametes versicolor TvJC209.

[0019] Figure 3 : Fruiting body diagram of Trametes versicolor TvJC209.

[0020] Figure 4 : Mycelial growth of different Trametes versicolor strains in a solid stock culture medium containing Miscanthus floridulus and Dicranopteris dichotoma.

[0021] Figure 5 : Mycelial growth of Trametes versicolor TvJC209 in a solid stock culture medium containing different Juncao.

[0022] Figure 6 : Fruiting body formation of Trametes versicolor strain TvJC209 in a cultivation medium containing Miscanthus floridulus and Dicranopteris dichotoma. Detailed implementation manners

[0023] To make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific implementation manners, but the present invention is not limited thereto.

[0024] Example 1: Wipe the surface of the fresh wild Trametes versicolor fruiting body with an alcohol cotton ball, cut the clean and pollution-free internal tissue from the torn surface with a disinfected scalpel and inoculate it on the mother culture medium. Place the inoculated petri dish in a constant temperature of 25 - 30 °C for 3 - 7 days. Break the grown mycelia and perform enzymatic hydrolysis using enzymes such as snailase and lysing enzyme to prepare single-cell protoplasts; Mutagenize the protoplasts using ultraviolet light with a wavelength of 302 nm (Vilber VLX-3D device); After the mutagenized protoplasts are cultured into mycelia, screening is carried out to obtain a new Trametes versicolor strain, namely Trametes versicolor TvJC209, which grows vigorously, has a fast growth rate, and is more suitable for Juncao cultivation; Among them, the formula of the mother culture medium is calculated by mass percentage as follows: potato flour 5%, glucose 2%, brown sugar 0.5%, agar powder 2%, defatted and desalted fish meal 0.3%, feed-grade yeast powder 0.2%, ferrous sulfate heptahydrate 0.007%, magnesium sulfate 0.2%, calcium sulfate heptahydrate 0.3%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.1%, and the balance is water. Trametes versicolor TvJC209 was deposited at the China Center for Type Culture Collection on June 19, 2023, classified and named as Trametes versicolor, with the deposit number CCTCC NO: M 20231064, and the deposit address is Wuhan University, Wuhan, China. The GenBank number of the characteristic sequence of Trametes versicolor TvJC209 in NCBI is OR058882.

[0025] Coriolus versicolor TvJC209 has the following characteristics: 1) There is a one-base difference (A at the 526th site → G at the 542nd site) between the ITS sequence of Coriolus versicolor TvJC209 and the ITS sequence of Trametes versicolor voucher ACAD21091F (OL741530.1) with the highest similarity in the NCBI database; 2) Coriolus versicolor TvJC209 can grow in a variety of dried or fresh mushroom grasses; 3) Under the sawdust cultivation conditions (the cultivation medium consists of sawdust, wheat bran, and gypsum in a mass ratio of 78:20:2, and the water content is adjusted to 55% - 65%), the polysaccharide content of the fruiting bodies can reach 4% - 5%, and the water-soluble extract content can reach 18% - 22%. Under the mushroom grass cultivation conditions (the cultivation medium consists of Miscanthus floridulus, Dicranopteris dichotoma, wheat bran, and gypsum in a mass ratio of 40:38:20:2, and the water content is adjusted to 55% - 65%), the polysaccharide content of the fruiting bodies can reach 4.5% - 6%, the water-soluble extract content can reach 18% - 22%, the triterpene content can reach 0.42%, and the biological conversion rate can reach 15% - 20%.

[0026] Figure 1 It is a mycelial plate diagram of Coriolus versicolor TvJC209.

[0027] Figure 2 It is a microscopic structure diagram of the mycelium of Coriolus versicolor TvJC209.

[0028] Figure 3 It is a fruiting body diagram of Coriolus versicolor TvJC209, with the front on the left and the back on the right.

[0029] Figure 4 It shows the mycelial growth of different Coriolus versicolor strains in the solid stock culture medium containing Miscanthus floridulus and Dicranopteris dichotoma. The leftmost one is Coriolus versicolor TvJC209.

[0030] Figure 5 It shows the mycelial growth of Coriolus versicolor TvJC209 in the solid stock culture medium containing different mushroom grasses. From left to right, the mushroom grasses in the culture medium are Pennisetum giganteum, Neyraudia reynaudiana, Phragmites australis, Cymbopogon citratus, Themeda villosa, Miscanthus sacchariflorus, Miscanthus floridulus, Sorghum propinquum, Arundo donax, and Pennisetum purpureum.

[0031] Figure 6 It shows the fruiting of Coriolus versicolor TvJC209 in the cultivation medium containing Miscanthus floridulus and Dicranopteris dichotoma (the cultivation medium consists of Miscanthus floridulus, Dicranopteris dichotoma, wheat bran, and gypsum in a mass ratio of 40:38:20:2, and the water content is adjusted to 55% - 65%).

[0032] Example 2: S1: Mother spawn preparation: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing mother spawn medium under aseptic conditions, and incubate it in the dark at 26°C for 7 - 14 days until the mycelium fills the test tube to obtain the mother spawn. Among them, the formula of the mother spawn medium by mass percentage is: potato flour 5%, glucose 2%, brown sugar 0.5%, agar powder 2%, defatted and desalted fish meal 0.3%, feed-grade yeast powder 0.2%, ferrous sulfate heptahydrate 0.007%, magnesium sulfate 0.2%, calcium sulfate heptahydrate 0.3%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.1%, and the balance is water.

[0033] S2: Original spawn preparation: Under aseptic conditions, inoculate 1 cm 2 of the mother spawn into a plastic bag containing solid original spawn medium, and incubate it in the dark at 26°C in an incubator until the mycelium fills the plastic bag to obtain the original spawn. Among them, the formula of the solid original spawn medium by mass percentage is: wheat 15%, sorghum 15%, corncob 15%, wheat bran 8%, brown sugar 0.2%, fish meal 0.2%, gypsum 0.5%, calcium oxide 0.5%, magnesium oxide 0.2%, ferrous sulfate heptahydrate 0.01%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and Arundo donax L. makes up the balance (specifically dry Arundo donax L.); adjust the water content to 62%.

[0034] S3: Cultivation and fruiting management: Under aseptic conditions, inoculate 1 - 2 g of the original spawn into a plastic bag containing cultivation material, transfer the inoculated plastic bag to the cultivation rack in the disinfected cultivation room, and incubate it in the dark at a temperature of 26°C and an air relative humidity of 65% - 75% until the mycelium fills the plastic bag; transfer the plastic bag with mature mycelium to the mushroom shed for fruiting management. During fruiting management, the mycelium is in full contact with the air, the temperature in the shed is 26°C, the air relative humidity is 80% - 90%, and scattered light and sufficient oxygen are provided in the shed. Cultivate for 10 - 20 days until the fruiting body matures, with the edge becoming thin and the white edge disappearing, and then pick it. Among them, the formula of the cultivation material by mass percentage is: wheat bran 20%, gypsum 1%, calcium oxide 1%, magnesium oxide 0.1%, ferrous sulfate heptahydrate 0.01%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and Arundo donax L. makes up the balance (specifically dry Arundo donax L.); adjust the water content to 62%.

[0035] Taking a plastic bag with 600 g of wet cultivation material as an example, the fresh weight of the first flush of mushrooms can reach 20 - 30 g / bag, and the fresh weight of the second flush of mushrooms can reach 10 - 18 g / bag.

[0036] Example 3: S1: Preparation of mother culture: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing mother culture medium according to aseptic operation, and incubate it in the dark at 25 °C for 7 - 14 days until the mycelium fills the test tube to obtain the mother culture. Among them, the formula of the mother culture medium by mass percentage is: potato powder 5%, glucose 2%, brown sugar 1%, agar powder 3%, defatted and desalted fish meal 0.5%, feed-grade yeast powder 0.3%, ferrous sulfate heptahydrate 0.007%, magnesium sulfate 0.2%, calcium sulfate heptahydrate 0.3%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, and the balance is water.

[0037] S2: Preparation of original species: Inoculate 1 cm 2 of the mother culture into a plastic bag containing solid original species medium according to aseptic operation, and incubate it in the dark at 25 °C in an incubator until the mycelium fills the plastic bag to obtain the original species. Among them, the formula of the solid original species medium by mass percentage is: wheat 15%, sorghum 15%, corncob 26.5%, wheat bran 5%, brown sugar 0.4%, fish meal 0.3%, gypsum 1%, calcium oxide 1%, magnesium oxide 0.4%, ferrous sulfate heptahydrate 0.015%, magnesium sulfate 0.1%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.1%, and Arundo donax L. makes up the balance (specifically dry Arundo donax L.); adjust the water content to 63%.

[0038] S3: Cultivation and management of fruiting bodies: Inoculate 1 - 2 g of the original species into a plastic bag containing cultivation material according to aseptic operation, transfer the inoculated plastic bag to the culture rack in the disinfected culture room, and incubate it in the dark at a temperature of 25 °C and a relative air humidity of 65% - 75% until the mycelium fills the plastic bag; transfer the plastic bag with mature mycelium to the mushroom shed for fruiting body management. During fruiting body management, the mycelium is in full contact with the air, the temperature in the shed is 25 °C, the relative air humidity is 80% - 90%, and scattered light and sufficient oxygen are provided in the shed. Cultivate for 10 - 20 days until the fruiting body is mature with a thin edge and the white edge disappears, and then pick it. Among them, the formula of the cultivation material by mass percentage is: rice bran 20%, gypsum 1%, calcium oxide 1.5%, magnesium oxide 0.1%, ferrous sulfate heptahydrate 0.01%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and Arundo donax L. makes up the balance (specifically, first use dry Arundo donax L. to make up the balance, and then calculate according to the water content of fresh Arundo donax L., and replace dry Arundo donax L. with fresh Arundo donax L. according to the principle of equal dry basis weight); adjust the water content to 63%.

[0039] Taking a plastic bag with a wet weight of 600 g of cultivation material as an example, the fresh weight of the first flush of fruiting bodies can reach 15 - 25 g / bag, and the fresh weight of the second flush of fruiting bodies can reach 8 - 13 g / bag.

[0040] Example 4: S1: Preparation of mother culture: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing mother culture medium according to aseptic operation, and incubate it in the dark at 26 °C for 7 - 14 days until the mycelium fills the test tube to obtain the mother culture. Among them, the formula of the mother culture medium by mass percentage is: potato powder 6%, glucose 2%, brown sugar 1%, agar powder 3%, defatted and desalted fish meal 0.4%, feed-grade yeast powder 0.3%, ferrous sulfate heptahydrate 0.007%, magnesium sulfate 0.1%, calcium sulfate heptahydrate 0.3%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and the balance is water.

[0041] S2: Preparation of original seed: Inoculate 1 cm 2 of the mother culture into a plastic bag containing solid original seed medium according to aseptic operation, and incubate it in the dark at 26 °C until the mycelium fills the plastic bag to obtain the original seed. Among them, the formula of the solid original seed medium by mass percentage is: wheat 15%, sorghum 15%, corncob 20%, wheat bran 8%, brown sugar 0.4%, fish meal 0.5%, gypsum 1.5%, calcium oxide 1%, magnesium oxide 0.3%, ferrous sulfate heptahydrate 0.01%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, and Pennisetum giganteum fills the balance (specifically dry Pennisetum giganteum); adjust the water content to 63%.

[0042] S3: Cultivation and fruiting management: Inoculate 1 - 2 g of the original seed into a plastic bag containing cultivation material according to aseptic operation, move the inoculated plastic bag to the cultivation rack in the disinfected cultivation room, and incubate it in the dark at a temperature of 25 °C and a relative air humidity of 65% - 75% until the mycelium fills the plastic bag; move the plastic bag with mature mycelium to the mushroom shed for fruiting management. During fruiting management, the mycelium is in full contact with the air, the temperature in the shed is 25 °C, the relative air humidity is 80% - 90%, and scattered light and sufficient oxygen are provided in the shed. Cultivate for 10 - 20 days until the fruiting body matures with a thin edge and the white edge disappears, and then pick it. Among them, the formula of the cultivation material by mass percentage is: wheat bran 20%, gypsum 1%, calcium oxide 1%, magnesium oxide 0.1%, ferrous sulfate heptahydrate 0.01%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and Pennisetum giganteum fills the balance (specifically, first fill the balance with dry Pennisetum giganteum, and then calculate according to the water content of fresh Pennisetum giganteum, and replace the dry Pennisetum giganteum with fresh Pennisetum giganteum according to the principle of equal dry basis weight); adjust the water content to 61%.

[0043] Taking a plastic bag with a wet weight of 600 g of cultivation material as an example, the fresh weight of the first flush of mushrooms can reach 13 - 20 g / bag, and the fresh weight of the second flush of mushrooms can reach 5 - 10 g / bag.

[0044] Example 5: S1: Mother culture preparation: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing the mother culture medium according to aseptic operation, and incubate it in the dark at 26 °C for 7 - 14 days until the mycelium fills the test tube to obtain the mother culture. Among them, the formula of the mother culture medium by mass percentage is: potato flour 6%, glucose 2%, brown sugar 1%, agar powder 2%, defatted and desalted fish meal 0.3%, feed-grade yeast powder 0.4%, ferrous sulfate heptahydrate 0.006%, magnesium sulfate 0.1%, calcium sulfate heptahydrate 0.3%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.2%, and the balance is water.

[0045] S2: Spawn preparation: Inoculate 1 cm 2 of the mother culture into a shaking flask containing the liquid spawn culture medium, and incubate it in the dark at 25 °C and 130 rpm on a shaker until mycelial pellets appear to obtain the spawn. Among them, the formula of the liquid spawn culture medium by mass percentage is: potato flour 2%, glucose 2%, brown sugar 1%, peptone 0.5%, yeast extract powder 0.2%, ferrous sulfate heptahydrate 0.001%, magnesium sulfate 0.1%, calcium sulfate heptahydrate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, and the balance is water.

[0046] S3: Cultivation and fruiting management: Inoculate 1 - 2 g of the spawn into a plastic bag containing the cultivation material according to aseptic operation, transfer the inoculated plastic bag to the cultivation rack in the disinfected cultivation room, and incubate it in the dark at a temperature of 25 °C and a relative air humidity of 65% - 75% until the mycelium fills the plastic bag; transfer the plastic bag with mature mycelium to the mushroom shed for fruiting management. During fruiting management, the mycelium is in full contact with the air, the temperature in the shed is 25 °C, the relative air humidity is 80% - 90%, and scattered light and sufficient oxygen are provided in the shed. Cultivate for 10 - 20 days until the fruiting body matures with a thin edge and the white edge disappears, and then pick it. Among them, the formula of the cultivation material by mass percentage is: wheat bran 20%, gypsum 1%, calcium oxide 1.5%, magnesium oxide 0.1%, ferrous sulfate heptahydrate 0.02%, magnesium sulfate 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.2%, and Pennisetum giganteum makes up the balance (specifically dry Pennisetum giganteum); adjust the water content to 63%.

[0047] Taking a plastic bag with a wet weight of 600 g of the cultivation material as an example, the fresh weight of the first flush of mushrooms can reach 18 - 28 g / bag, and the fresh weight of the second flush of mushrooms can reach 10 - 15 g / bag.

[0048] The above are only the preferred embodiments of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope covered by the present invention.

Claims

1. Coriolus versicolor TvJC209, characterized in that: The classification name is Coriolus versicolor ( Trametes versicolor ), which was deposited at the China Center for Type Culture Collection on June 19, 2023, with the deposit number CCTCC NO: M 20231064 and the deposit address being Wuhan University, Wuhan, China.

2. The method for cultivating Coriolus versicolor TvJC209 using Juncao as claimed in claim 1, characterized in that: It includes the following steps: S1: Inoculate the Trametes versicolor TvJC209 strain into a test tube containing the mother culture medium, and incubate it in the dark in an incubator at 25 - 30°C until the mycelium fills the test tube to obtain the mother culture; S2: Inoculate the mother culture into a plastic bag containing the solid original culture medium, and incubate it in the dark in an incubator at 25 - 30°C until the mycelium fills the plastic bag to obtain the original culture; or inoculate the mother culture into a shake flask containing the liquid original culture medium, and incubate it in the dark on a shaker at a temperature of 25 - 30°C and a rotation speed of 120 - 160 rpm until mycelial pellets appear to obtain the original culture; S3: Inoculate the original culture into a plastic bag containing the cultivation material, and incubate it in the dark in a culture room at a temperature of 25 - 30°C and a relative air humidity of 65% - 75% until the mycelium fills the plastic bag; transfer the plastic bag with mature mycelium development to the mushroom shed for fruiting management. During fruiting management, the mycelium is in full contact with the air, the temperature in the shed is 25 - 28°C, the relative air humidity is 80% - 90%, and scattered light and sufficient oxygen are provided in the shed, and cultivate until the fruiting body matures.

3. The method for cultivating Juncao according to claim 2, wherein: The formula of the mother culture medium is calculated by mass percentage as follows: potato flour 3% - 7%, glucose 1% - 2.5%, brown sugar 0.5% - 1%, agar powder 1.5% - 3%, defatted and desalted fish meal 0.2% - 0.5%, feed - grade yeast powder 0.1% - 0.4%, ferrous sulfate heptahydrate 0.001% - 0.01%, magnesium sulfate 0.1% - 0.2%, calcium sulfate heptahydrate 0.2% - 0.5%, sodium chloride 0.1% - 0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1% - 0.2%, and the balance is water.

4. The method for cultivating Juncao according to claim 2, wherein: The formula of the solid original culture medium is: wheat 5% - 15%, sorghum 5% - 15%, corncob 15% - 30%, wheat bran 5% - 10%, brown sugar 0.1% - 0.5%, fish meal 0.1% - 0.5%, gypsum 0.5% - 1.5%, calcium oxide 0.5% - 1.5%, magnesium oxide 0.2% - 0.5%, ferrous sulfate heptahydrate 0.002% - 0.02%, magnesium sulfate 0.1% - 0.2%, sodium chloride 0.1% - 0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1% - 0.2%, and dry mushroom grass makes up the balance; adjust the water content to 55% - 65%.

5. The method for cultivating Juncao according to claim 4, wherein: The mushroom grass is any one or more of Arundo donax, Pennisetum giganteum, Neyraudia reynaudiana, Phragmites australis, Cymbopogon citratus, Themeda villosa, Miscanthus sacchariflorus, Miscanthus floridulus, Sorghum propinquum, Pennisetum purpureum, Dicranopteris dichotoma; calculated according to the water content of fresh mushroom grass, and in accordance with the principle of equal dry - basis weight, the dry mushroom grass can be replaced by fresh mushroom grass.

6. The method for cultivating Juncao according to claim 2, characterized in that: The formula of the liquid original culture medium is calculated by mass percentage as follows: potato flour 1% - 3%, glucose 1% - 3%, brown sugar 0.5% - 1%, peptone 0.1% - 0.6%, yeast extract powder 0.1% - 0.4%, ferrous sulfate heptahydrate 0.001% - 0.01%, magnesium sulfate 0.1% - 0.2%, calcium sulfate heptahydrate 0.1% - 0.2%, sodium chloride 0.1% - 0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1% - 0.2%, and the balance is water.

7. The method for cultivating Juncao according to claim 2, characterized in that: The formula of the cultivation material is as follows by mass percentage: wheat bran or rice bran 10% - 20%, gypsum 0.5% - 2%, calcium oxide 0.5% - 1.5%, magnesium oxide 0.1% - 0.2%, ferrous sulfate heptahydrate 0.002% - 0.02%, magnesium sulfate 0.1% - 0.2%, sodium chloride 0.1% - 0.2%, potassium dihydrogen phosphate or dipotassium hydrogen phosphate 0.1% - 0.2%, and dry mushroom grass makes up the balance; adjust the water content to 55% - 65%.

8. The method for cultivating Juncao according to claim 7, characterized in that: The mushroom grass is any one or more of Arundo donax, Pennisetum giganteum, Neyraudia reynaudiana, Phragmites australis, Cymbopogon citratus, Themeda villosa, Miscanthus sacchariflorus, Miscanthus floridulus, Sorghum propinquum, Pennisetum purpureum, Dicranopteris dichotoma; calculated based on the water content of fresh mushroom grass, according to the principle of equal dry basis weight, the dry mushroom grass can be replaced by fresh mushroom grass.

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