Repair method, device, equipment, medium and product of sequencing flow cell

Abstract

The invention provides a repairing method, device and equipment for a sequencing flow groove, a medium and a product, and relates to the technical field of gene sequencing. The method comprises the following steps: determining a damage type of a target sequencing flow cell; if the damage type is a preset repairable type, a corresponding repair scheme is determined based on the preset repairable type, and the repair scheme comprises cleaning treatment or cleaning treatment and repair treatment; and controlling a corresponding operation component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell. According to the method, automatic repairing of the sequencing flow groove is achieved, corresponding repairing schemes are adopted for different types of sequencing flow grooves, all kinds of sequencing flow grooves can be accurately repaired, the cleanliness degree of the treated sequencing flow grooves is greatly improved, and the service life of the sequencing flow grooves is greatly prolonged under the condition that the good use performance of the sequencing flow grooves is guaranteed. And the service life is prolonged.

Description

Technical Field

[0001] The present application relates to the technical field of gene sequencing, and particularly to a method, device, equipment, medium and product for repairing a sequencing flow cell. Background Art

[0002] A sequencing flow cell is a core component in a gene sequencing device, and oligonucleotide fragments complementary to sequencing adapters are fixed on its surface for adsorbing and amplifying DNA fragments.

[0003] To ensure the accuracy of sequencing results, the sequencing flow cell is usually a disposable consumable, but the cost of the flow cell is relatively high. If it is replaced immediately after use, it will cause waste of resources.

[0004] To save the usage cost, a series of cleaning, disinfection and repair means are manually adopted, including ultrasonic cleaning, soaking in acid-base solutions, high-temperature and high-pressure sterilization, grinding and repair, etc., so that the sequencing flow cell can be reused.

[0005] However, the existing means are usually repaired manually, lacking automated means; and there are situations where incomplete cleaning affects subsequent experimental results and causes cross-contamination, and the repair effect is not ideal, affecting the performance and service life of the consumables. Summary of the Invention

[0006] The present application provides a method, device, equipment, medium and product for repairing a sequencing flow cell to solve the technical problems of lacking automated means, incomplete cleaning and unsatisfactory repair effect.

[0007] In a first aspect, the present application provides a method for repairing a sequencing flow cell, the method comprising:

[0008] Determining the damage type of a target sequencing flow cell;

[0009] If the damage type is a preset repairable type, determining a corresponding repair plan based on the preset repairable type, the repair plan including a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell;

[0010] Controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell.

[0011] In a possible design, the preset repairable types include a first repairable type and a second repairable type. Among them, the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; determining a corresponding repair plan based on the preset repairable type includes:

[0012] If the preset repairable type is the first repairable type, determine that the corresponding repair plan is a cleaning process;

[0013] If the preset repairable type is the second repairable type, determine that the corresponding repair plan is a cleaning process and a repair process.

[0014] In a possible design, the repair plan includes a cleaning process. Controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes:

[0015] Control the temperature control component to set the temperature to a first preset temperature;

[0016] After responding to reaching the first preset temperature, control the grasping component to place the target sequencing flow cell in the cleaning container of the enzymatic cleaning solution, and clean the target sequencing flow cell with the enzymatic cleaning solution;

[0017] After responding to determining that cleaning the target sequencing flow cell with the enzymatic cleaning solution reaches a first preset duration, control the grasping component to move the target sequencing flow cell to the cleaning container of the oxidative cleaning solution, and control the ultrasonic component to turn on, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

[0018] In a possible design, the cleaning process further includes determining the residue concentration of the target sequencing flow cell. Controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes:

[0019] Control the on-line ultraviolet absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell;

[0020] Receive the first detection result sent by the on-line ultraviolet absorbance detector and the fluorescence detector;

[0021] Determine the residue concentration of the target sequencing flow cell according to the first detection result.

[0022] In a possible design, the repair plan includes a repair process. Controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes:

[0023] Control the grasping component to place the target sequencing flow cell in a plasma surface activation processor for activation processing;

[0024] After the duration of the activation processing reaches the second preset duration, control the spraying component to spray a repair liquid on the surface of the target sequencing flow cell;

[0025] And / or, control the grasping component to place the target sequencing flow cell in a preset mold, and control the temperature to be the second preset temperature and the pressure to be a preset pressure value to perform hot pressing and shaping on the target sequencing flow cell.

[0026] In a possible design, before determining the damage type of the target sequencing flow cell, the method further includes:

[0027] Control a computer vision sensor to collect an image of the target sequencing flow cell;

[0028] The determining of the damage type of the target sequencing flow cell includes:

[0029] Receive the image sent by the computer vision sensor, where the image includes the target sequencing flow cell;

[0030] Determine the damage type of the target sequencing flow cell based on the image.

[0031] In a possible design, the determining of the damage type of the target sequencing flow cell based on the image includes:

[0032] Use a preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image;

[0033] If the image includes surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the second repairable type;

[0034] If the image does not include surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the first repairable type.

[0035] In a possible design, the training steps of the preset damage type determination model include:

[0036] Obtain a training sample set, where the training sample set includes image samples of multiple sequencing flow cells, and the image samples of the multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image;

[0037] Use the training sample set to train an initial damage type determination model;

[0038] In response to meeting the model convergence condition, determine the initial damage type determination model that meets the model convergence condition as the preset damage type determination model.

[0039] In a possible design, the first preset temperature is 35°C to 45°C;

[0040] And / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate;

[0041] And / or, the oxidative cleaning solution includes hydrogen peroxide and citric acid.

[0042] In a possible design, before determining the damage type of the target sequencing flow cell, the method further includes:

[0043] Control the cleaning component to remove the residual sealing film and sealant on the surface of the target sequencing flow cell.

[0044] In a possible design, after controlling the corresponding operation component to repair the target sequencing flow cell based on the repair scheme to obtain the repaired sequencing flow cell, the method further includes:

[0045] Control the optical detector and the sequencing verification instrument to detect the repaired sequencing flow cell;

[0046] Receive the second detection result sent by the optical detector and the sequencing verification instrument;

[0047] Determine whether the repair is successful according to the second detection result;

[0048] The indicators for the detection include at least two of the following:

[0049] Flatness, residue concentration, light transmittance, surface roughness, output data volume, base error rate.

[0050] In a second aspect, the present application provides a repair device for a sequencing flow cell, and the device includes:

[0051] A determination module, configured to determine the damage type of the target sequencing flow cell;

[0052] The determining module is further configured to, if the damage type is a preset repairable type, determine a corresponding repair solution based on the preset repairable type, where the repair solution includes a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell;

[0053] The control module is configured to control a corresponding operating component to operate on the target sequencing flow cell based on the repair solution, so as to obtain a repaired sequencing flow cell.

[0054] In a third aspect, the present application provides a repair device for a sequencing flow cell, the device including: a processor, and a memory communicatively connected to the processor;

[0055] The memory is used to store computer execution instructions;

[0056] The processor is configured to execute the computer execution instructions stored in the memory to implement the method according to any one of the first aspect.

[0057] In a fourth aspect, the present application provides a computer-readable storage medium, where computer execution instructions are stored in the computer-readable storage medium, and when the computer execution instructions are executed by a processor, they are used to implement the method according to any one of the first aspect.

[0058] In a fifth aspect, the present application provides a computer program product, including a computer program, and when the computer program is executed by a processor, it implements the method according to any one of the first aspect.

[0059] The repair method, device, equipment, medium and product of the sequencing flow cell provided by this application determine the damage type of the target sequencing flow cell; if the damage type is a preset repairable type, a corresponding repair plan is determined based on the preset repairable type, and the repair plan includes a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell. By determining the damage type of the target sequencing flow cell, the most appropriate repair measures can be taken for different damage situations, avoiding resource waste caused by blind repair and possible secondary damage to the flow cell, and improving the repair efficiency and success rate; the preset repairable types and their corresponding repair plans standardize and streamline the repair process; in the cleaning process, first cleaning with an enzymatic cleaning solution at a specific temperature can effectively decompose and remove organic residues on the surface of the target sequencing flow cell, and then further cleaning with an oxidative cleaning solution until the residue concentration is lower than the preset threshold, ensuring the thorough cleaning of the flow cell. The two-step cleaning strategy combines the advantages of enzymatic hydrolysis and oxidation, improves the cleaning efficiency and effect, and reduces sequencing errors caused by residues; after the residue concentration meets the requirements, a repair process is carried out. The solution provides options for coating repair and / or hot pressing shaping to deal with different types of physical damage. Coating repair can restore the surface performance of the flow cell, while hot pressing shaping can correct structural problems caused by physical deformation, improving the repair utilization rate and lifespan of the flow cell; controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan realizes the automation and intelligence of the repair process, not only reducing manual intervention, reducing operation errors, but also improving the repair efficiency and consistency; through the above series of precise and efficient repair measures, the finally obtained repaired sequencing flow cell can significantly improve the accuracy and reliability of sequencing and achieve recycling. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] The accompanying drawings herein are incorporated into the specification and constitute a part of this specification, showing embodiments consistent with this application, and are used together with the specification to explain the principles of this application.

[0061] Figure 1 It is an application scenario diagram of the repair method of the sequencing flow cell provided by an embodiment of this application;

[0062] Figure 2Flow chart of the repair method of the sequencing flow cell provided by an embodiment of the present application;

[0063] Figure 3 Flow chart of the repair method of the sequencing flow cell provided by another embodiment of the present application;

[0064] Figure 4 Structural schematic diagram of the repair device of the sequencing flow cell provided by an embodiment of the present application;

[0065] Figure 5 Structural schematic diagram of the repair equipment of the sequencing flow cell provided by an embodiment of the present application.

[0066] Through the above-mentioned drawings, specific embodiments of the present application have been shown, and there will be more detailed descriptions hereinafter. These drawings and textual descriptions are not intended to limit the scope of the concept of the present application in any way, but to illustrate the concept of the present application to those skilled in the art by referring to specific embodiments. Detailed implementation manners

[0067] Here, the exemplary embodiments will be described in detail, and the examples are shown in the drawings. When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The implementation manners described in the following exemplary embodiments do not represent all the implementation manners consistent with the present application. On the contrary, they are only examples of the devices and methods consistent with some aspects of the present application as detailed in the appended claims.

[0068] It should be noted that in the embodiments of the present application, some software, components, and models may be mentioned. They should be regarded as exemplary, and their purpose is only to illustrate the feasibility in the implementation of the technical solution of the present application, but it does not mean that the applicant has already or necessarily used this solution.

[0069] To clearly understand the technical solution of the present application, the solutions of the prior art will be introduced in detail first.

[0070] In the field of gene sequencing, a sequencing flow cell is a core component in gene sequencing equipment. Oligonucleotide fragments complementary to sequencing adapters are fixed on its surface for adsorbing and amplifying DNA fragments, and its performance directly affects the sequencing quality and efficiency. With the rapid development of high-throughput sequencing technology, the requirements for the accuracy and stability of flow cells are increasing. However, during the use of flow cells, problems such as physical damage, chemical residues, or impurity accumulation often occur, resulting in attenuation of sequencing signals, increased error rates, and affecting sequencing accuracy. Existing repair solutions mostly focus on a series of manual cleaning, disinfection, and repair means, including ultrasonic cleaning, soaking in acid-base solutions, high-temperature and high-pressure sterilization, grinding repair, etc. However, these methods often have difficulty in completely removing stubborn residues, may cause secondary damage to the surface of the flow cell, reduce its service life, and lack standardized and automated processes, making it difficult to ensure the consistency and reliability of repair effects.

[0071] Therefore, when facing the technical problems in the prior art, to improve the accuracy of repair measures, first determine the damage type of the target sequencing flow cell, and determine the corresponding repair plan according to different damage types, which not only improves the repair efficiency, but also presets the repairable types and their corresponding repair plans, making the repair process standardized and procedural; to improve the cleaning effect, an enzymatic cleaning solution and an oxidative cleaning solution are used for two-step cleaning, combining the advantages of enzymatic hydrolysis and oxidation, improving the cleaning efficiency and effect, and reducing sequencing errors caused by residues; since the sequencing flow cell may also have different degrees of physical damage, different repair treatments are set, including coating repair and / or hot pressing shaping, to adapt to various damage types, improving the repair utilization rate and service life of the flow cell; to reduce manual intervention and reduce operation errors, based on the repair plan, the corresponding operating components are controlled to operate on the target sequencing flow cell, realizing the automation and intelligence of the repair process, ensuring the consistency and reliability of the repair effect, and achieving recycling.

[0072] Figure 1 The application scenario diagram of the repair method of the sequencing flow cell provided by an embodiment of the present application is as Figure 1 shown. The application scenario diagram of the repair method of the sequencing flow cell provided by this embodiment includes: a server 101 and an operating device 102. It can be understood that a repair device for the sequencing flow cell is integrated in the server 101 for executing the repair method of the sequencing flow cell provided by the present application; all operating components required in the repair plan are included in the operating device 102; the server 101 is communicatively connected to the operating device 102.

[0073] Optionally, the repair device for the sequencing flow cell may also be integrated in a device including all operating components, and this embodiment does not limit this.

[0074] Specifically, the server 101 first determines the damage type of the target sequencing flow cell. If the damage type is a preset repairable type, a corresponding repair plan is determined based on the preset repairable type. The repair plan includes a cleaning process or a cleaning process and a repair process. Then, the server 101 controls the corresponding operating components in the operating device 102 to operate on the target sequencing flow cell based on the repair plan, so as to obtain a repaired sequencing flow cell.

[0075] Optionally, the server 101 may send a control instruction to the operating device 102, so that after receiving the control instruction, the operating device 102 controls the corresponding operating components to operate. It may also be to control the corresponding operating components to operate in other ways, and this embodiment does not limit this.

[0076] Optionally, the operating device 102 can be used to collect data of the target sequencing flow cell and send the relevant data to the server 101, so that the server 101 determines the damage type of the target sequencing flow cell based on the relevant data; or other methods can be used to determine the damage type of the target sequencing flow cell, and this embodiment does not limit this.

[0077] The following uses specific embodiments to describe in detail the technical solution of the present application and how the technical solution of the present application solves the above technical problems. The following several specific embodiments can be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments. The embodiments of the present application will be described below with reference to the drawings.

[0078] Figure 2 It is a flowchart of a method for repairing a sequencing flow cell provided by an embodiment of the present application. As Figure 2 shown, the execution subject of this embodiment is a repair device for a sequencing flow cell. The repair device for the sequencing flow cell can be implemented by a computer program, or can be implemented by a medium storing relevant computer programs, such as a USB flash drive and / or an optical disc, etc., and can also be integrated in a repair device for a sequencing flow cell, such as a server, etc. The method for repairing a sequencing flow cell provided by this embodiment includes the following steps:

[0079] Step 201, determine the damage type of the target sequencing flow cell.

[0080] Among them, the target sequencing flow cell refers to the sequencing flow cell that needs to be repaired.

[0081] It can be understood that different damages may occur to the sequencing flow cell during use, such as contamination, scratches, deformation, etc.

[0082] Exemplarily, an image of the target sequencing flow cell sent by the image acquisition device can be received, such as images of various surfaces of the target sequencing flow cell. The received image of the target sequencing flow cell is compared with the reference sequencing flow cell image pre-stored in the repair device of the sequencing flow cell, so as to determine the damage type of the target sequencing flow cell.

[0083] Among them, the reference sequencing flow cell image refers to an image of a brand-new undamaged sequencing flow cell.

[0084] Optionally, the damage type of the target sequencing flow cell can be determined by calculating the pixel difference vector between the reference sequencing flow cell image and the target sequencing flow cell image and then comparing it with a preset difference threshold, or other comparison methods can also be used. This embodiment does not limit this.

[0085] Optionally, a pre-trained model can also be used to determine the damage type of the target sequencing flow cell according to the image of the target sequencing flow cell. This embodiment does not limit this.

[0086] It can be understood that, in order to improve the accuracy of determining the damage type, before determining the damage type of the target sequencing flow cell, the film and sealant residues on the surface of the target sequencing flow cell can be removed.

[0087] Optionally, before determining the damage type of the target sequencing flow cell, the target sequencing flow cell can be identified by controlling a spectral sensor, such as identifying the code on the surface of the target sequencing flow cell or judging characteristics such as the material of the flow cell according to the laser reflection characteristics, as a label for the target sequencing flow cell to be used for identification and distinction, or the target sequencing flow cell can be distinguished by other means. This embodiment does not limit this.

[0088] Step 202, if the damage type is a preset repairable type, then determine the corresponding repair plan based on the preset repairable type. The repair plan includes a cleaning process or a cleaning process and a repair process; among them, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell.

[0089] Among them, the preset repairable type refers to the type name that can be repaired and is pre-configured in the repair device of the sequencing flow cell, such as the first type, the second type, etc.

[0090] Among them, the first preset temperature refers to the temperature during the cleaning process, which is pre-configured in the repair device of the sequencing flow cell, such as 40°C, 45°C, etc. This embodiment does not limit this.

[0091] Among them, the first preset duration refers to the duration of cleaning with the enzymatic cleaning solution, which is pre-configured in the repair device of the sequencing flow cell, such as 10 minutes, 20 minutes, etc., and this embodiment does not limit it.

[0092] Among them, the preset residue concentration threshold refers to the value preset in the repair device of the sequencing flow cell for determining whether the residue concentration on the surface of the target sequencing flow cell meets the expectation, such as 0.1 ng / mm², etc., and this embodiment does not limit it.

[0093] Among them, the enzymatic cleaning solution refers to a liquid containing specific enzymes for catalyzing hydrolysis reactions of proteins, polysaccharides, etc.

[0094] Among them, the oxidative cleaning solution refers to a liquid containing a certain amount of oxidant for decomposing trace elements.

[0095] Specifically, after determining the damage type of the target sequencing flow cell, compare this type with the preset repairable types. If the damage type is a preset repairable type, the specific repair plan can be determined based on the mapping relationship between the repairable type and the repair plan preset in the repair device of the sequencing flow cell.

[0096] Exemplarily, if it is determined that the damage type of the target sequencing flow cell is the first type, and the preset repairable types include the first type and the second type, then it is determined that the damage type of the target sequencing flow cell is a preset repairable type. Since the mapping relationship between the repairable type and the repair plan is pre-configured in the repair device of the sequencing flow cell, such as the first type corresponding to cleaning treatment and the second type corresponding to cleaning treatment plus repair treatment, the repair plan corresponding to the target sequencing flow cell is determined to be cleaning treatment according to this mapping relationship.

[0097] It can be understood that after determining the damage type of the target sequencing flow cell, there may still be an irreparable type, such as the third type, which is not among the preset repairable types. Then, the target sequencing flow cell cannot be repaired, and other treatments can be performed on it, such as disassembling the sequencing flow cell and extracting raw materials, or discarding it, etc., and this embodiment does not limit it.

[0098] Optionally, during the cleaning process, the residue concentration on the surface of the target sequencing flow cell can be detected in real time through measures such as fluorescence detection until the residue concentration is less than the preset residue concentration threshold; or other methods can be used for detection, and this embodiment does not limit it.

[0099] Step 203, control the corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain the repaired sequencing flow cell.

[0100] Exemplarily, the repair solution is a cleaning process and a repair process. When starting to determine the damage type, the target sequencing flow cell can be placed on a conveyor belt. After determining the damage type, the conveyor belt is controlled to move it to a specified position. For example, it is first moved to the cleaning process position, the temperature control component is controlled to set the temperature to a first preset temperature, and then the nozzle is controlled to start spraying an enzymatic cleaning solution so that the target sequencing flow cell can be cleaned by the enzymatic cleaning solution at the first preset temperature until reaching a first preset duration. Then, the nozzle is controlled to stop spraying the enzymatic cleaning solution and start spraying an oxidative cleaning solution so that the target sequencing flow cell can be cleaned by the oxidative cleaning solution at the first preset temperature until the residue concentration is less than a preset residue concentration threshold. Then, the conveyor belt is controlled to move the cleaned target sequencing flow cell to the repair process position, and the spraying component is controlled to spray a repair solution on the surface of the target sequencing flow cell and / or the shaping component is controlled to perform hot pressing on the target sequencing flow cell according to the repair solution.

[0101] Optionally, the nozzle for spraying the enzymatic cleaning solution and the oxidative cleaning solution can be the same or different, and this embodiment does not limit this.

[0102] It should be noted that the two cleaning solutions cannot be mixed. That is, if the same nozzle is used for spraying, the two cleaning solutions should be placed in different containers to avoid interference with the cleaning effect after mixing.

[0103] Optionally, it can also be to control other components such as a robotic arm to move the target sequencing flow cell to a specified position, and this embodiment does not limit this.

[0104] Optionally, each target sequencing flow cell can be processed individually, or multiple target sequencing flow cells can be processed in batches simultaneously, and this embodiment does not limit this.

[0105] It can be understood that the above are all exemplary descriptions, and it can also be to perform specific operations by controlling other specific operating components, and this embodiment does not limit this.

[0106] The repair method of the sequencing flow cell provided by the embodiment of the present application determines the damage type of the target sequencing flow cell; if the damage type is a preset repairable type, a corresponding repair plan is determined based on the preset repairable type, and the repair plan includes a cleaning treatment or a cleaning treatment and a repair treatment; wherein, the cleaning treatment includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair treatment includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell. By determining the damage type of the target sequencing flow cell, the most appropriate repair measures can be taken for different damage situations, avoiding the waste of resources caused by blind repair and the possible secondary damage to the flow cell, and improving the repair efficiency and success rate; the preset repairable types and their corresponding repair plans standardize and streamline the repair process; in the cleaning treatment, first cleaning with an enzymatic cleaning solution at a specific temperature can effectively decompose and remove the organic residues on the surface of the target sequencing flow cell, and then further cleaning with an oxidative cleaning solution until the residue concentration is lower than the preset threshold, ensuring the thorough cleaning of the flow cell. The two-step cleaning strategy combines the advantages of enzymatic hydrolysis and oxidation, improves the cleaning efficiency and effect, and reduces the sequencing errors caused by residues; after the residue concentration meets the requirements, the repair treatment is carried out. The solution provides options for coating repair and / or hot pressing shaping to deal with different types of physical damage. Coating repair can restore the surface performance of the flow cell, while hot pressing shaping can correct the structural problems caused by physical deformation, improving the repair utilization rate and lifespan of the flow cell; controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan realizes the automation and intelligence of the repair process, not only reducing manual intervention, lowering operation errors, but also improving the repair efficiency and consistency; through the above series of precise and efficient repair measures, the finally obtained repaired sequencing flow cell can significantly improve the accuracy and reliability of sequencing and achieve recycling.

[0107] As an optional implementation manner, on the basis of the above embodiment, the preset repairable types include a first repairable type and a second repairable type, wherein, the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; determining the corresponding repair plan based on the preset repairable type includes:

[0108] If the preset repairable type is the first repairable type, determining the corresponding repair plan as a cleaning treatment;

[0109] If the preset repairable type is the second repairable type, determine that the corresponding repair solution is cleaning treatment and repair treatment.

[0110] Among them, physical damage can include surface scratches, structural deformation, etc.

[0111] It can be understood that the surface of the sequencing flow cell will definitely be contaminated after use, such as the presence of proteins, fluorescent dyes, metal ions, etc.

[0112] Specifically, if the preset repairable type is the first repairable type, that is, the target sequencing flow cell has no physical damage and only surface contamination, then determine that the repair solution is cleaning treatment, and just clean the target sequencing flow cell to remove surface contamination; if the preset repairable type is the second repairable type, that is, the target sequencing flow cell not only has surface contamination but also physical damage, then determine that the repair solution is cleaning treatment and repair treatment. First, clean the target sequencing flow cell to remove surface contamination, and then perform repair treatment to improve the physical damage.

[0113] The repair method of the sequencing flow cell provided by the embodiment of the present application, the preset repairable types include the first repairable type and the second repairable type. Among them, the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; determine the corresponding repair solution based on the preset repairable type, including: if the preset repairable type is the first repairable type, determine that the corresponding repair solution is cleaning treatment; if the preset repairable type is the second repairable type, determine that the corresponding repair solution is cleaning treatment and repair treatment. Adopting a differentiated repair solution for different repairable types can accurately respond to the damage condition of the target sequencing flow cell. For the first repairable type, only perform cleaning treatment to restore its normal sequencing function and avoid unnecessary damage caused by over-repair; for the second repairable type, add repair treatment on the basis of cleaning treatment to repair physical damage specifically, so that the flow cell can be restored to a state close to the original state, ensuring the accuracy and reliability of sequencing; the accurate repair solution avoids secondary damage to the sequencing flow cell caused by inappropriate repair operations and improves the repair efficiency of the sequencing flow cell.

[0114] As an optional implementation manner, on the basis of the above embodiment, the repair solution includes cleaning treatment, and control the corresponding operating components to operate on the target sequencing flow cell based on the repair solution, including:

[0115] Control the temperature control component to set the temperature to the first preset temperature;

[0116] After responding to reaching the first preset temperature, control the grasping component to place the target sequencing flow cell in the cleaning container of the enzymatic cleaning solution, and clean the target sequencing flow cell with the enzymatic cleaning solution;

[0117] After determining that the cleaning of the target sequencing flow cell with the enzymatic cleaning solution reaches the first preset duration, control the grasping component to move the target sequencing flow cell into the cleaning container of the oxidative cleaning solution, and control the ultrasonic component to start, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

[0118] Among them, the temperature control component refers to a component used to monitor, control, and adjust temperature, such as a temperature controller, etc., and this embodiment does not limit this.

[0119] Among them, the grasping component refers to a component used to grasp an object and move the object to a specified position, such as a robotic arm, etc., and this embodiment does not limit this.

[0120] Among them, the ultrasonic component refers to a component used to emit ultrasonic waves, such as an ultrasonic generator, etc., and this embodiment does not limit this.

[0121] Among them, the vibration frequency of ultrasonic waves can be pre-configured in the repair device of the sequencing flow cell to control the ultrasonic component to vibrate at this frequency, such as 40 kHz, and this embodiment does not limit this.

[0122] It can be understood that the ultrasonic component emits ultrasonic waves to the cleaning container of the oxidative cleaning solution, so that the target sequencing flow cell is cleaned under the vibration of ultrasonic waves.

[0123] Specifically, when performing the cleaning process, first control the temperature control component to set the temperature to the first preset temperature, so that the target sequencing flow cell can be cleaned at the first preset temperature. When the temperature reaches the first preset temperature, control the grasping component to move the target sequencing flow cell into the cleaning container of the enzymatic cleaning solution, and control the container containing the enzymatic cleaning solution to start flowing out the enzymatic cleaning solution, such as spraying the cleaning solution with a nozzle, so that the target sequencing flow cell is rinsed by the enzymatic cleaning solution in the cleaning container; when the duration of cleaning the target sequencing flow cell with the enzymatic cleaning solution reaches the first preset duration, control the container containing the enzymatic cleaning solution to stop flowing out the enzymatic cleaning solution, and control the grasping component to move the target sequencing flow cell into the cleaning container of the oxidative cleaning solution. At the same time, control the ultrasonic component to start at the preset vibration frequency and the container containing the oxidative cleaning solution to start flowing out the oxidative cleaning solution, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

[0124] Optionally, during the cleaning process, the real-time detection component can be controlled to detect the residue concentration of the liquid in the cleaning container, such as detecting the content of DNA and protein by ultraviolet light, or detecting the signal intensity of the fluorescent dye at a wavelength of Ex / Em = 488 / 520 nm, etc., until the residue concentration is less than the preset residue concentration threshold, and this embodiment does not limit this.

[0125] The repair method of the sequencing flow cell provided by the embodiment of the present application includes, at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution, including: controlling the temperature control component to set the temperature to the first preset temperature; after reaching the first preset temperature, controlling the grasping component to place the target sequencing flow cell in the cleaning container of the enzymatic cleaning solution, and cleaning the target sequencing flow cell with the enzymatic cleaning solution; after determining that the cleaning of the target sequencing flow cell with the enzymatic cleaning solution reaches the first preset duration, controlling the grasping component to move the target sequencing flow cell to the cleaning container of the oxidative cleaning solution, and controlling the ultrasonic component to be turned on so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment. By controlling the temperature control component, the grasping component, etc. for operation, an automated process is realized, making the repair work of the sequencing flow cell standardized and improving the efficiency and reliability of the repair; when performing oxidative cleaning, the ultrasonic component is turned on, so that the impurities attached to the surface and tiny gaps of the sequencing flow cell are accelerated to fall off, improving the cleaning effect.

[0126] As an optional implementation manner, on the basis of the above embodiment, the cleaning process further includes determining the residue concentration of the target sequencing flow cell and controlling the corresponding operation components to operate on the target sequencing flow cell based on the repair plan, including:

[0127] Controlling the on-line ultraviolet absorption detector and the fluorescence detector to detect the surface of the target sequencing flow cell;

[0128] Receiving the first detection results sent by the on-line ultraviolet absorption detector and the fluorescence detector;

[0129] Determining the residue concentration of the target sequencing flow cell according to the first detection results.

[0130] Wherein, the first detection results refer to the data sent by the on-line ultraviolet absorption detector and the fluorescence detector, including the relevant data for calculating the residue concentration.

[0131] Specifically, after cleaning with the oxidative cleaning solution for a certain duration, controlling the extraction component to extract the residue sample on the surface of the target sequencing flow cell and place it in the on-line ultraviolet absorption detector and the fluorescence detector for detection. After the on-line ultraviolet absorption detector and the fluorescence detector complete the detection, they send the detection results to the sequencing flow cell repair device. After receiving the first detection results, the sequencing flow cell repair device calculates the residue concentration of the target sequencing flow cell according to the pre-configured calculation formula.

[0132] Optionally, it may also be to control the on-line ultraviolet absorption detector and the fluorescence detector to perform imaging detection on the surface of the target sequencing flow cell, and this embodiment does not limit this.

[0133] Optionally, if the residue concentration is greater than the preset residue concentration threshold, the cleaning can be repeated until the residue concentration is less than the preset residue concentration threshold.

[0134] In the repair method of the sequencing flow cell provided by the embodiments of the present application, the cleaning process further includes: controlling an on-line ultraviolet absorbance detector and a fluorescence detector to detect the surface of the target sequencing flow cell; receiving the first detection result sent by the on-line ultraviolet absorbance detector and the fluorescence detector; and determining the residue concentration of the target sequencing flow cell according to the first detection result. By using the on-line ultraviolet absorbance detector and the fluorescence detector, the surface of the target sequencing flow cell can be detected in real time and accurately, and the concentration of the residue can be accurately quantified, which helps to judge whether the cleaning reaches the expected effect and avoid affecting the accuracy and reliability of subsequent sequencing due to excessive residues. The automated and intelligent control can not only improve the cleaning efficiency, but also reduce the influence of human factors on the cleaning quality, making the cleaning process more stable and reliable.

[0135] As an optional implementation manner, on the basis of the above embodiment, the repair plan includes a repair process, and controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan, including:

[0136] Controlling the grasping component to place the target sequencing flow cell in a plasma surface activation processor for activation processing;

[0137] After the duration of the activation process reaches the second preset duration, controlling the spraying component to spray a repair liquid on the surface of the target sequencing flow cell;

[0138] And / or, controlling the grasping component to place the target sequencing flow cell in a preset mold, and controlling the temperature to be the second preset temperature and the pressure to be the preset pressure value to perform hot pressing and shaping on the target sequencing flow cell.

[0139] Wherein, the grasping component refers to a component used to grasp the target sequencing flow cell and move it to a specified position, such as a vacuum suction cup, a parallel gripper, etc., and this embodiment does not limit this.

[0140] Wherein, the second preset duration refers to the duration of the activation process, which is pre-configured in the repair device of the sequencing flow cell, such as 20s, 30s, etc., and this embodiment does not limit this.

[0141] Wherein, the preset mold is a mold used to perform hot pressing and shaping on the target sequencing flow cell, and its size is the same as that of the standard sequencing flow cell, so that the target sequencing flow cell can be restored to its original shape.

[0142] Wherein, the second preset temperature is the temperature of the hot pressing and shaping, which is pre-configured in the repair device of the sequencing flow cell, such as 80°C, 90°C, etc., and this embodiment does not limit this.

[0143] Among them, the preset pressure value refers to the pressure value applied during hot press shaping, which is pre-configured in the repair device of the sequencing flow cell, such as 10 kPa, 20 kPa, etc. This embodiment does not limit this.

[0144] Specifically, after the cleaning process, if coating repair is required, control the grasping component to move the target sequencing flow cell to the plasma surface activation processor and control the plasma surface activation processor to start running according to parameters such as the pre-configured activation power to perform activation processing on the target sequencing flow cell. When the duration of the activation processing reaches the second preset duration, control the grasping component to take out the activated target flow cell from the plasma surface activation processor and place it at the spraying position, and then control the spraying component to spray the repair liquid on the surface of the target sequencing flow cell to repair the scratches on the target sequencing flow cell; if hot press shaping is required, control the grasping component to move the target sequencing flow cell into the preset mold and control the temperature and pressure of the preset mold according to the second preset temperature and the preset pressure value, so that the target sequencing flow cell can be hot press shaped under this condition.

[0145] Among them, the activation processing can be carried out in an argon atmosphere, and the activation power can be 50 w. This embodiment does not limit this.

[0146] Among them, the repair liquid can be a silicon-based nano repair liquid, and the spraying thickness can be set to ≤200 nm. This embodiment does not limit this.

[0147] Among them, the processing time of hot press shaping can be 5 min, and the geometric dimension error can be controlled within 0.1 μm / mm. This embodiment does not limit this.

[0148] The repair method of the sequencing flow cell provided by the embodiment of the present application performs coating repair and / or hot pressing and shaping on the target sequencing flow cell, including: controlling the grasping component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment; after the duration of the activation treatment reaches the second preset duration, controlling the spraying component to spray a repair liquid on the surface of the target sequencing flow cell; and / or, controlling the grasping component to place the target sequencing flow cell in a preset mold, and controlling the temperature to be the second preset temperature and the pressure to be the preset pressure value to perform hot pressing and shaping on the target sequencing flow cell. Performing activation treatment on the target sequencing flow cell can form a large number of active groups on the surface, enhancing the adhesion between the coating and the surface of the sequencing flow cell; controlling the spraying component to spray a repair liquid on the surface of the target sequencing flow cell after activation treatment can accurately control the spraying flow rate, speed, and angle according to actual needs, further ensuring the uniformity of the coating, so that the surface of the target sequencing flow cell restores optical flatness and hydrophilic properties; placing the target sequencing flow cell in a preset mold and controlling the temperature to be the second preset temperature and the pressure to be the preset pressure value for hot pressing and shaping, the shape of the mold can precisely determine the final shape of the flow cell, thereby achieving precise repair of the shape of the sequencing flow cell and ensuring platform compatibility.

[0149] As an optional implementation manner, on the basis of the above embodiment, before determining the damage type of the target sequencing flow cell, the method further includes:

[0150] Controlling a computer vision sensor to collect images of the target sequencing flow cell;

[0151] Determining the damage type of the target sequencing flow cell includes:

[0152] Receiving the image sent by the computer vision sensor, where the image includes the target sequencing flow cell;

[0153] Based on the image, determining the damage type of the target sequencing flow cell.

[0154] Wherein, the image sent by the computer vision sensor may include images of each surface of the target sequencing flow cell.

[0155] Optionally, the computer vision sensor may be a camera, a camera, etc., and the present embodiment does not limit this.

[0156] Specifically, before determining the damage type of the target sequencing flow cell, first control the computer vision sensor to collect images of the target sequencing flow cell, such as collecting images of each surface of the target sequencing flow cell, and then pack and send them to the repair device of the sequencing flow cell. After the repair device of the sequencing flow cell receives the image sent by the computer vision sensor, it determines the damage type of the target sequencing flow cell based on the image.

[0157] Exemplarily, an artificial intelligence image recognition system can be pre-configured in the repair device of the sequencing flow cell to determine the damage type of the target sequencing flow cell based on the image; or the received image can be pixel-compared with the graph of the original sequencing flow cell to determine the damage type, and this embodiment does not limit this.

[0158] The repair method of the sequencing flow cell provided by the embodiment of the present application determines the damage type of the target sequencing flow cell, including: controlling a computer vision sensor to collect an image of the target sequencing flow cell; receiving the image sent by the computer vision sensor, where the image includes the target sequencing flow cell; and determining the damage type of the target sequencing flow cell based on the image. The computer vision sensor can collect images of the target sequencing flow cell at an extremely high speed, improving the classification efficiency compared with manual detection, and the image has high resolution and can capture the subtle damage on the surface of the target sequencing flow cell, ensuring the accuracy when determining the damage type of the target sequencing flow cell based on the image; combining the computer vision sensor with the repair device of the sequencing flow cell can realize an automated process for determining the damage type of the sequencing flow cell.

[0159] As an alternative implementation, on the basis of the above embodiment, determining the damage type of the target sequencing flow cell based on the image includes:

[0160] Using a preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image;

[0161] If the image includes surface scratches and / or structural deformations, then determine that the damage type of the target sequencing flow cell is the second repairable type;

[0162] If the image does not include surface scratches and / or structural deformations, then determine that the damage type of the target sequencing flow cell is the first repairable type.

[0163] Among them, the preset damage type determination model refers to a model pre-configured in the repair device of the sequencing flow cell for determining the damage type of the target sequencing flow cell based on the image, such as an open-source deep learning model, etc.

[0164] Specifically, after receiving the image of the target sequencing flow cell, input it into the preset damage type determination model. The preset damage type determination model can judge whether the current image includes surface scratches and / or structural deformations according to the pre-trained memory. If the image includes surface scratches and / or structural deformations, then determine that the damage type of the target sequencing flow cell is the second repairable type. If the image does not include surface scratches and / or structural deformations, then determine that the damage type of the target sequencing flow cell is the first repairable type.

[0165] It can be understood that the preset damage type determination model is a trained model obtained through a large number of image recognition learnings.

[0166] The repair method of the sequencing flow cell provided by the embodiment of the present application determines the damage type of the target sequencing flow cell based on an image, including: using the preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image; if the image includes surface scratches and / or structural deformations, determining that the damage type of the target sequencing flow cell is the second repairable type; if the image does not include surface scratches and / or structural deformations, determining that the damage type of the target sequencing flow cell is the first repairable type. The preset damage type determination model can quickly analyze and judge based on the input image. Compared with manual detection that requires experience and long-term observation, the classification time is greatly shortened; the preset damage type determination model analyzes the image based on unified standards and algorithms, ensuring the consistency and accuracy of the results; by judging whether the image includes surface scratches and / or structural deformations, the damage type is clearly divided into the second repairable type and the first repairable type, providing clear guidance for the repair work, thereby improving the repair success rate.

[0167] As an alternative implementation, based on the above embodiment, the training steps of the preset damage type determination model include:

[0168] Obtain a training sample set, where the training sample set includes image samples of multiple sequencing flow cells, and the image samples of multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image;

[0169] Use the training sample set to train the initial damage type determination model;

[0170] In response to meeting the model convergence condition, determine the initial damage type determination model that meets the model convergence condition as the preset damage type determination model.

[0171] Specifically, after collecting image samples of multiple sequencing flow cells using a computer vision sensor, mark the surface scratches and / or structural deformations among them, and merge them with the images without surface scratches and structural deformations as the training sample set, and input the training sample set into the initial damage type determination model, so that the initial damage type determination model learns to judge the image features including surface scratches and / or structural deformations based on the training sample set. During the training process, the parameters can be adjusted in a timely manner, and it is judged whether the model convergence condition is met. When the model convergence condition is met, the initial damage type determination model that meets the model convergence condition is determined as the preset damage type determination model.

[0172] Among them, the model convergence condition may be that the number of iterations reaches a preset number of iterations, or other model convergence conditions, which are not limited in this embodiment.

[0173] The training step of the preset damage type determination model in the sequencing flow cell repair method provided by the embodiment of the present application includes: obtaining a training sample set, where the training sample set includes image samples of multiple sequencing flow cells, and the image samples of multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image; training the initial damage type determination model with the training sample set; in response to meeting the model convergence condition, determining the initial damage type determination model that meets the model convergence condition as the preset damage type determination model. By training the initial damage type determination model with a training sample set including image samples of multiple sequencing flow cells, where the image samples of multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image, the model can learn the characteristics of surface scratches and / or structural deformations in the image, so that when determining the damage type, it can quickly determine whether the image includes surface scratches and / or structural deformations, and then determine the damage type.

[0174] As an optional implementation manner, on the basis of the above embodiment, the first preset temperature is 35°C to 45°C;

[0175] And / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate;

[0176] And / or, the oxidative cleaning solution includes hydrogen peroxide and citric acid.

[0177] Among them, the enzymatic cleaning solution may be a cleaning solution containing proteinase K with a concentration of 0.1 mg / mL, sodium dodecyl sulfate with a content of 0.05%, and pH = 7.5, or other ratios, which are not limited in this embodiment.

[0178] Among them, the oxidative cleaning solution may contain 、2% citric acid and a cleaning solution with pH = 4.0, or other ratios, which are not limited in this embodiment.

[0179] The repair method of the sequencing flow cell provided by the embodiment of the present application, the first preset temperature is 35°C to 45°C; and / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate; and / or, the oxidative cleaning solution includes hydrogen peroxide and citric acid. Controlling the temperature at 35°C to 45°C can achieve low-temperature cleaning and avoid damaging the material structure of the sequencing flow cell at high temperature; proteinase K is specifically used to cut the protein residues in the contaminants, and sodium dodecyl sulfate is used to assist in destroying the DNA-protein complex structure, thereby realizing the stripping of the contaminated layer; the combination of hydrogen peroxide and citric acid can effectively decompose fluorophores and residual metal ions, further remove trace contaminants, and prevent interference from sequencing background signals.

[0180] As an alternative implementation manner, on the basis of the above embodiment, after determining the damage type of the target sequencing flow cell, the method further includes:

[0181] Controlling the cleaning component to remove the residual film and sealant on the surface of the target sequencing flow cell.

[0182] Among them, the cleaning component can be a robotic arm including a tool head, and the present embodiment does not limit this.

[0183] Specifically, after determining the damage type of the target sequencing flow cell, control the robotic arm and control the pressure within The robotic arm will gently scrape off the residual film, glue or label on the surface of the target sequencing flow cell with a special tool head.

[0184] Before determining the damage type of the target sequencing flow cell in the repair method of the sequencing flow cell provided by the embodiment of the present application, the method further includes: controlling the cleaning component to remove the residual film and sealant on the surface of the target sequencing flow cell. The residual film and sealant may partially cover the key parts of the sequencing flow cell, resulting in inaccurate identification of the damage state of the sequencing flow cell and possible secondary damage in subsequent processing. Removing these residues can improve the accuracy of the damage type determination result and the repair success rate.

[0185] As an alternative implementation manner, on the basis of the above embodiment, after controlling the corresponding operation component to repair the target sequencing flow cell according to the repair plan to obtain the repaired sequencing flow cell, the method further includes:

[0186] Controlling an optical detector and a sequencing verification instrument to detect the repaired sequencing flow cell;

[0187] Receiving the second detection result sent by the optical detector and the sequencing verification instrument;

[0188] Determining whether the repair is successful according to the second detection result;

[0189] The detection indexes include at least two of the following:

[0190] Flatness, residue concentration, light transmittance, surface roughness, output data volume, base error rate.

[0191] Among them, the second detection result refers to the detection data sent by the optical detector and the sequencing verifier to verify whether the repair is successful.

[0192] Specifically, after obtaining the repaired sequencing flow cell, control the grasping component to move the repaired sequencing flow cell into the optical detector, and control the optical detector to detect the surface of the repaired sequencing flow cell. After the detection is completed, then control the grasping component to move the repaired sequencing flow cell into the sequencing verifier, and control the sequencing detector to detect the repaired sequencing flow cell using the standard human genomic DNA sample as a template. After the detection is completed, the optical detector and the sequencing verifier send the detection results to the repair device of the sequencing flow cell. After receiving the second detection result, the repair device of the sequencing flow cell determines whether the repair is successful according to the second detection result, such as determining whether the surface roughness of the repaired sequencing flow cell meets the preset threshold, etc.

[0193] Optionally, the detection results of the optical detector and the sequencing verifier detecting a brand-new sequencing flow cell under the same test conditions can be pre-stored in the repair device of the sequencing flow cell for comparison with the second detection result to determine whether the repair is successful, or thresholds for each detection index can be pre-configured in the repair device of the sequencing flow cell to determine whether the repair is successful. This embodiment does not limit this.

[0194] Exemplarily, set the surface roughness Ra difference ≤ 0.01 μm, the light transmittance is not less than 90% of the new sequencing flow cell, and the output data volume is not less than 92% of the new sequencing flow cell, etc.

[0195] As shown in Table 1, it is a comparison of the detection data of the new sequencing flow cell and the repaired sequencing flow cell.

[0196] Table 1

[0197]

[0198] It can be seen from Table 1 that the repaired sequencing flow cell is close to the new sequencing flow cell in terms of light transmittance, surface roughness, and output data volume, which can prove that the sequencing flow cell obtained by repairing using the method provided in this application has good quality and function and has the performance of repeated reuse.

[0199] The repair method for the sequencing flow cell provided by the embodiment of the present application controls the corresponding operating components to repair the target sequencing flow cell based on the repair plan. After obtaining the repaired sequencing flow cell, the method further includes: controlling an optical detector and a sequencing verifier to detect the repaired sequencing flow cell; receiving the second detection results sent by the optical detector and the sequencing verifier; determining whether the repair is successful according to the second detection results; the detection indicators include at least two of the following: flatness, residue concentration, light transmittance, surface roughness, output data volume, base error rate. By controlling the optical detector and the sequencing verifier to perform the detection and determining whether the repair is successful according to the detection results, unqualified sequencing flow cells can be found in time, ensuring the usability of the repaired sequencing flow cell. The detection indicators include at least two of flatness, residue concentration, light transmittance, surface roughness, output data volume, and base error rate, and the repair situation of the sequencing flow cell can be determined from multiple dimensions, improving the reliability of the result of determining whether the repair is successful.

[0200] Figure 3 The flowchart of the repair method for the sequencing flow cell provided by another embodiment of the present application is as Figure 3 shown. The repair method for the sequencing flow cell provided by this embodiment is that the repair plan includes a cleaning process and a repair process, and the repair process includes a coating repair and a hot pressing and shaping. The specific steps are as follows. The drug target prediction method provided by this embodiment includes the following steps:

[0201] Step 301, control the cleaning component to remove the residual sealing film and sealant on the surface of the target sequencing flow cell.

[0202] Step 302, control the computer vision sensor to collect an image of the target sequencing flow cell.

[0203] Step 303, receive the image sent by the computer vision sensor, and the image includes the target sequencing flow cell.

[0204] Step 304, use the preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image.

[0205] Step 305, in response to the image including surface scratches and structural deformation, determine that the damage type of the target sequencing flow cell is the second repairable type.

[0206] Step 306, in response to determining that the second repairable type is the preset repairable type, determine the corresponding repair plan as a cleaning process and a repair process.

[0207] Step 307, control the temperature control component to set the temperature to the first preset temperature.

[0208] Step 308, in response to reaching the first preset temperature, control the grasping component to place the target sequencing flow cell into the cleaning container of the enzymatic cleaning solution, and clean the target sequencing flow cell with the enzymatic cleaning solution.

[0209] Step 309, in response to determining that the cleaning of the target sequencing flow cell with the enzymatic cleaning solution has reached the first preset duration, control the grasping component to move the target sequencing flow cell to the cleaning container of the oxidative cleaning solution, and control the ultrasonic component to turn on, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

[0210] Step 310, control the on-line ultraviolet absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell. Step 311, receive the first detection result sent by the on-line ultraviolet absorbance detector and the fluorescence detector.

[0211] Step 312, determine the residue concentration of the target sequencing flow cell according to the first detection result.

[0212] Step 313, in response to the residue concentration being less than the preset residue concentration threshold, control the grasping component to place the target sequencing flow cell into the plasma surface activation processor for activation treatment.

[0213] Step 314, in response to the activation treatment duration reaching the second preset duration, control the spraying component to spray the repair solution on the surface of the target sequencing flow cell.

[0214] Step 315, control the grasping component to place the target sequencing flow cell into a preset mold, and control the temperature to the second preset temperature and the pressure to the preset pressure value to perform hot pressing and shaping on the target sequencing flow cell to obtain the repaired sequencing flow cell.

[0215] Step 316, control the optical detector and the sequencing verifier to detect the repaired sequencing flow cell.

[0216] Step 317, receive the second detection result sent by the optical detector and the sequencing verifier.

[0217] Step 318, determine whether the repair is successful according to the second detection result.

[0218] It can be understood that if the residue concentration determined in step 312 is greater than the preset residue concentration threshold, continue to repeat step 308 and step 309 until the residue concentration is less than the preset residue concentration threshold.

[0219] In this embodiment, the implementation manners and technical effects of steps 301 - 318 are similar to those of the corresponding solutions in the above embodiments, and will not be elaborated here.

[0220] Figure 4The structural schematic diagram of the repair device for the sequencing flow cell provided by an embodiment of the present application is as follows Figure 4 As shown, the repair device for the sequencing flow cell provided by this embodiment is located in the repair equipment of the sequencing flow cell. Then, the repair device 40 for the sequencing flow cell provided by this embodiment includes: a determination module 41 and a control module 42.

[0221] Among them, the determination module 41 is used to determine the damage type of the target sequencing flow cell; the determination module 41 is also used to, if the damage type is a preset repairable type, determine the corresponding repair plan based on the preset repairable type, and the repair plan includes a cleaning process or a cleaning process and a repair process; among them, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; the control module 42 is used to control the corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell.

[0222] The repair device for the sequencing flow cell provided by this embodiment can execute Figure 2 the method provided by the embodiment. The specific implementation principle and technical effect are similar and will not be elaborated here.

[0223] Optionally, the preset repairable types include a first repairable type and a second repairable type. Among them, the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage. When the determination module 41 determines the corresponding repair plan based on the preset repairable type, it is specifically used to: if the preset repairable type is the first repairable type, determine the corresponding repair plan as a cleaning process; if the preset repairable type is the second repairable type, determine the corresponding repair plan as a cleaning process and a repair process.

[0224] Optionally, the repair plan includes a cleaning process. When the control module 42 controls the corresponding operating components to operate on the target sequencing flow cell based on the repair plan, it is specifically used to: control the temperature control component to set the temperature to the first preset temperature; in response to reaching the first preset temperature, control the grasping component to place the target sequencing flow cell in the cleaning container of the enzymatic cleaning solution, and clean the target sequencing flow cell with the enzymatic cleaning solution; in response to determining that the cleaning of the target sequencing flow cell with the enzymatic cleaning solution reaches the first preset duration, control the grasping component to move the target sequencing flow cell to the cleaning container of the oxidative cleaning solution, and control the ultrasonic component to start, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

[0225] Optionally, the cleaning process further includes determining the residue concentration of the target sequencing flow cell. The control module 42, when controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan, is specifically configured to: control the on-line ultraviolet absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell; receive the first detection results sent by the on-line ultraviolet absorbance detector and the fluorescence detector; and determine the residue concentration of the target sequencing flow cell according to the first detection results.

[0226] Optionally, the repair plan includes a repair process. The control module 42, when controlling the corresponding operating components to operate on the target sequencing flow cell based on the repair plan, is specifically configured to: control the grasping component to place the target sequencing flow cell in the plasma surface activation processor for activation processing; in response to the duration of the activation processing reaching the second preset duration, control the spraying component to spray a repair liquid on the surface of the target sequencing flow cell; and / or control the grasping component to place the target sequencing flow cell in a preset mold, and control the temperature to be the second preset temperature and the pressure to be the preset pressure value to perform hot pressing and shaping on the target sequencing flow cell.

[0227] Optionally, the control module 42 is further configured to control the computer vision sensor to collect an image of the target sequencing flow cell. The determination module 41, when determining the damage type of the target sequencing flow cell, is specifically configured to: receive the image sent by the computer vision sensor, where the image includes the target sequencing flow cell; and determine the damage type of the target sequencing flow cell based on the image.

[0228] Optionally, when determining the damage type of the target sequencing flow cell based on the image, the determination module 41 is specifically configured to: use a preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image; if the image includes surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the second repairable type; if the image does not include surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the first repairable type.

[0229] Optionally, the repair device for the sequencing flow cell provided in this embodiment further includes a training module.

[0230] Correspondingly, the training module is configured to obtain a training sample set, where the training sample set includes image samples of multiple sequencing flow cells. The image samples of the multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image; use the training sample set to train an initial damage type determination model; and in response to meeting the model convergence condition, determine the initial damage type determination model that meets the model convergence condition as the preset damage type determination model.

[0231] Optionally, the first preset temperature is 34°C to 44°C; and / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate; and / or, the oxidative cleaning solution includes hydrogen peroxide and citric acid.

[0232] Optionally, the control module 42 is further configured to control the cleaning component to remove the residual film and sealant on the surface of the target sequencing flow cell.

[0233] Optionally, the repair device for the sequencing flow cell provided in this embodiment further includes a receiving module.

[0234] Correspondingly, the control module 42 is further configured to control the optical detector and the sequencing verifier to detect the repaired sequencing flow cell; the receiving module is configured to receive the second detection result sent by the optical detector and the sequencing verifier; the determination module 41 is further configured to determine whether the repair is successful according to the second detection result; the detection indexes include at least one of the following: flatness, residue concentration, light transmittance, surface roughness, output data volume, base error rate.

[0235] Figure 5 FIG. is a schematic structural diagram of a repair device for a sequencing flow cell provided in an embodiment of the present application, as Figure 5 shown, the repair device 50 for the sequencing flow cell provided in this embodiment includes: a processor 51 and a memory 52 communicatively connected to the processor.

[0236] Wherein, the memory 52 is used to store computer execution instructions; the processor 51 executes the computer execution instructions stored in the memory 52 to implement the repair method for the sequencing flow cell provided in the above embodiment. Relevant descriptions can be understood by corresponding to the relevant descriptions and effects of the steps in the accompanying drawings, and will not be elaborated here.

[0237] Wherein, the program may include program codes, and the program codes include computer execution instructions. The memory 52 may include a high-speed RAM memory, and may also include non-volatile memory, such as at least one disk memory.

[0238] Wherein, in this embodiment, the processor 51 and the memory 52 are connected by a bus. The bus may be an Industry Standard Architecture (ISA) bus, a Peripheral Component Interconnect (PCI) bus, or an Extended Industry Standard Architecture (EISA) bus, etc. The bus can be divided into an address bus, a data bus, a control bus, etc. For the sake of representation, Figure 5It is represented only by a thick line, but it does not mean that there is only one bus or one type of bus.

[0239] The embodiments of the present application further provide a computer-readable storage medium, in which computer-executable instructions are stored. When the controller executes the computer-executable instructions, each step in the method in the above embodiments is implemented.

[0240] The embodiments of the present application further provide a computer program product, including a computer program. When the computer program is executed by the controller, each step in the method in the above embodiments is implemented.

[0241] The various embodiments described above of the present application can be implemented in digital electronic circuit systems, integrated circuit systems, field programmable gate arrays (FPGAs), application specific integrated circuits (ASICs), application specific standard parts (ASSPs), systems on chip (SOCs), complex programmable logic devices (CPLDs), computer hardware, firmware, software, and / or combinations thereof. These various embodiments can include: implemented in one or more computer programs, which can be executed and / or interpreted on a programmable system including at least one programmable processor. The programmable processor can be a dedicated or general-purpose programmable processor, and can receive data and instructions from a storage system, at least one input device, and at least one output device, and transmit the data and instructions to the storage system, the at least one input device, and the at least one output device.

[0242] The computer-executable instructions for implementing the method of the present application can be written in any combination of one or more programming languages. These computer-executable instructions can be provided to the processor or controller of a general-purpose computer, a special-purpose computer, or other programmable data processing devices, such that when the computer-executable instructions are executed by the processor or controller, the functions / operations specified in the flowchart and / or block diagram are implemented. The computer-executable instructions can be executed entirely on the machine, partially on the machine, executed partially on the machine as an independent software package and partially on a remote machine, or executed entirely on a remote machine or electronic device.

[0243] In the context of the present application, a computer-readable storage medium can be a tangible medium that can contain or store a program for use by or in connection with an instruction execution system, apparatus, or device. A computer-readable storage medium can be a machine-readable signal medium or a machine-readable storage medium. A computer-readable storage medium can include, but is not limited to, electronic, magnetic, optical, electromagnetic, infrared, or semiconductor systems, apparatus, or devices, or any suitable combination of the foregoing. More specific examples of a computer-readable storage medium include an electrical connection based on one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read only memory (ROM), an erasable programmable read only memory (EPROM), an optical fiber, a portable compact disc read only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing. Alternatively, a computer-readable storage medium can include: resistive random access memory (RRAM), dynamic random access memory (DRAM), static random access memory (SRAM), enhanced dynamic random access memory (EDRAM), high-bandwidth memory (HBM), hybrid memory cube (HMC), and so on.

[0244] The systems and techniques described herein can be implemented in a computing system that includes back-end components (e.g., as a data electronic device), or a computing system that includes middleware components (e.g., an application electronic device), or a computing system that includes front-end components (e.g., a user computer having a graphical user interface or a web browser through which a user can interact with an implementation of the systems and techniques described herein), or a computing system that includes any combination of such back-end components, middleware components, or front-end components. The components of the system can be interconnected to each other by any form or medium of digital data communication (e.g., a communication network). Examples of a communication network include: a local area network (LAN), a wide area network (WAN), and the Internet.

[0245] It should be noted that, for the foregoing method embodiments, for the sake of simple description, they are all expressed as a series of action combinations. However, those skilled in the art should know that this application is not limited by the described action sequence, because according to this application, certain steps can be performed in other sequences or simultaneously. Secondly, those skilled in the art should also know that the embodiments described in the specification are all optional embodiments, and the actions and modules involved are not necessarily essential to this application. In other words, the various forms of processes shown above can be used, with steps reordered, added, or deleted. For example, the steps described in the disclosure of this application can be executed in parallel, sequentially, or in different orders, as long as the desired results of the technical solution disclosed in this application can be achieved, and this is not limited herein.

[0246] It should be further noted that although the steps in the flowchart are shown sequentially according to the arrows, these steps are not necessarily executed in the order indicated by the arrows. Unless there is a clear description in this article, the execution of these steps has no strict order limit, and these steps can be executed in other orders. Moreover, at least some of the steps in the flowchart may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily executed at the same time, but can be executed at different times, and the execution order of these sub-steps or stages is not necessarily sequential, but can be executed alternately or in turn with at least a part of other steps or sub-steps or stages of other steps.

[0247] It should be understood that the above device embodiments are illustrative only, and the devices of this application can also be implemented in other ways. For example, the division of units / modules in the above embodiments is only a logical function division, and there can be other division methods in actual implementation. For example, multiple units, modules, or components can be combined, or can be integrated into another system, or some features can be ignored or not executed.

[0248] In addition, without special instructions, in each embodiment of this application, each functional unit / module can be integrated in one unit / module, or each unit / module can exist physically alone, or two or more units / modules can be integrated together. The above integrated unit / module can be implemented in the form of hardware or in the form of a software program module.

[0249] When the integrated unit / module is implemented in the form of hardware, the hardware can be a digital circuit, an analog circuit, etc. The physical implementation of the hardware structure includes but is not limited to transistors, memristors, etc.

[0250] If the integrated unit / module is implemented in the form of a software program module and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, in essence, or the part that contributes to the related technology, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a memory and includes several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods of the various embodiments of the present application. And the aforementioned memory includes: various media such as USB flash drives, read-only memories ROM, random access memories RAM, mobile hard disks, magnetic disks, or optical discs that can store computer-executable instructions.

[0251] In the above embodiments, the descriptions of the various embodiments have their own emphases. For the parts not detailed in a certain embodiment, reference can be made to the relevant descriptions of other embodiments. The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered to be within the scope described in this specification.

[0252] Those skilled in the art will readily think of other implementation manners of the present application after considering the specification and practicing the invention disclosed herein. The present application is intended to cover any variations, uses, or adaptations of the present application, which follow the general principles of the present application and include the common general knowledge or conventional technical means in the technical field not disclosed in the present application. The specification and the embodiments are only regarded as exemplary.

[0253] It should be understood that the present application is not limited to the exact structure already described and shown in the drawings, and various modifications and changes can be made without departing from its scope. Therefore, the above specific implementation manners do not constitute a limitation to the protection scope of the present application. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the principles of the present application should be included within the protection scope of the present application.

Claims

1. A repair method for a sequencing flow cell, characterized in that, The method includes: Determining the damage type of the target sequencing flow cell; If the damage type is a preset repairable type, determining a corresponding repair plan based on the preset repairable type, where the repair plan includes a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; Controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell.

2. The method according to claim 1, characterized in that, The preset repairable types include a first repairable type and a second repairable type, where the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; determining a corresponding repair plan based on the preset repairable type includes: If the preset repairable type is the first repairable type, determining the corresponding repair plan as a cleaning process; If the preset repairable type is the second repairable type, determining the corresponding repair plan as a cleaning process and a repair process.

3. The method according to claim 1, wherein The repair plan includes a cleaning process, and controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes: Controlling a temperature control component to set the temperature to the first preset temperature; In response to reaching the first preset temperature, controlling a grasping component to place the target sequencing flow cell in a cleaning container of the enzymatic cleaning solution and cleaning the target sequencing flow cell with the enzymatic cleaning solution; In response to determining that the cleaning of the target sequencing flow cell with the enzymatic cleaning solution reaches the first preset duration, controlling the grasping component to move the target sequencing flow cell to a cleaning container of the oxidative cleaning solution and controlling an ultrasonic component to be turned on so that the target sequencing flow cell is cleaned by the oxidative cleaning solution in an ultrasonic environment.

4. The method according to claim 1, characterized in that The cleaning process further includes determining the residue concentration of the target sequencing flow cell, and controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes: Controlling an on-line ultraviolet absorbance detector and a fluorescence detector to detect the surface of the target sequencing flow cell; Receiving a first detection result sent by the on-line ultraviolet absorbance detector and the fluorescence detector; Determining the residue concentration of the target sequencing flow cell according to the first detection result.

5. The method according to claim 1, wherein The repair plan includes a repair process, and controlling corresponding operating components to operate on the target sequencing flow cell based on the repair plan includes: Controlling a grasping component to place the target sequencing flow cell in a plasma surface activation processor for activation processing; In response to the activation processing duration reaching a second preset duration, controlling a spraying component to spray a repair solution on the surface of the target sequencing flow cell; And / or, control the grasping component to place the target sequencing flow cell in a preset mold, and control the temperature to a second preset temperature and the pressure to a preset pressure value to perform hot pressing and shaping on the target sequencing flow cell.

6. The method according to claim 1 or 2, characterized in that Before determining the damage type of the target sequencing flow cell, the method further includes: Controlling a computer vision sensor to collect an image of the target sequencing flow cell; Determining the damage type of the target sequencing flow cell includes: Receiving the image sent by the computer vision sensor, where the image includes the target sequencing flow cell; Determining the damage type of the target sequencing flow cell based on the image.

7. The method according to claim 6, wherein Determining the damage type of the target sequencing flow cell based on the image includes: Using a preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image; If the image includes surface scratches and / or structural deformations, determining that the damage type of the target sequencing flow cell is the second repairable type; If the image does not include surface scratches and / or structural deformations, determining that the damage type of the target sequencing flow cell is the first repairable type.

8. The method according to claim 7, wherein The training steps of the preset damage type determination model include: Obtaining a training sample set, where the training sample set includes image samples of multiple sequencing flow cells, and the image samples of the multiple sequencing flow cells include samples with surface scratches and / or structural deformations in the image and samples without surface scratches and structural deformations in the image; Using the training sample set to train an initial damage type determination model; In response to meeting the model convergence condition, determining the initial damage type determination model that meets the model convergence condition as the preset damage type determination model.

9. The method according to claim 1, characterized in that, The first preset temperature is 35°C to 45°C; And / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate; And / or, the oxidative cleaning solution includes hydrogen peroxide and citric acid.

10. The method according to claim 1, wherein Before determining the damage type of the target sequencing flow cell, the method further includes: Controlling a cleaning component to remove the remaining film and sealant on the surface of the target sequencing flow cell.

11. The method according to claim 1, wherein, After controlling the corresponding operating component to repair the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell, the method further includes: Controlling an optical detector and a sequencing verification instrument to detect the repaired sequencing flow cell; Receiving a second detection result sent by the optical detector and the sequencing verification instrument; Determining whether the repair is successful according to the second detection result; The indicators for the detection include at least two of the following: Flatness, residue concentration, light transmittance, surface roughness, output data volume, base error rate.

12. A repair device for a sequencing flow cell, characterized in that, The device includes: A determination module for determining the damage type of the target sequencing flow cell; The determining module is further configured to, if the damage type is a preset repairable type, determine a corresponding repair solution based on the preset repairable type, where the repair solution includes a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a first preset temperature, cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset duration, and then cleaning the target sequencing flow cell with an oxidative cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; The control module is configured to control a corresponding operating component to operate on the target sequencing flow cell based on the repair solution, so as to obtain a repaired sequencing flow cell.

13. A repair device for a sequencing flow cell, characterized in that, The device includes: a processor, and a memory communicatively connected to the processor; The memory is configured to store computer execution instructions; The processor is configured to execute the computer execution instructions stored in the memory to implement the method according to any one of claims 1-11.

14. A computer-readable storage medium, characterized in that, Computer execution instructions are stored in the computer-readable storage medium, and when the computer execution instructions are executed by a processor, they are used to implement the method according to any one of claims 1-11.

15. A computer program product, including a computer program, where when the computer program is executed by a processor, it implements the method according to any one of claims 1-11.

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