Cell transfection enrichment degree control method based on environmental element adjustment

Abstract

The invention discloses a cell transfection enrichment degree control method based on environmental element adjustment, which comprises the following steps: establishing a plurality of groups of environmental element sets based on environmental requirements of cell transfection; recording the execution time of the cell transfection operation based on the environmental element set; obtaining the cell density within the execution time of the cell transfection operation, and obtaining the theoretical transfection cell density based on the cell density curve; acquiring actual transfection cell density based on the theoretical transfection cell density, and acquiring a cell transfection enrichment degree corresponding to the actual transfection cell density; obtaining the ratio of the cell transfection enrichment degree to the actual transfection cell density to obtain an enrichment proportion; obtaining an environment element set corresponding to the enrichment proportion, and obtaining a to-be-selected environment element set; obtaining to-be-selected environmental element sets of different stages of cell transfection, and establishing a cell transfection environmental element chain. The problem that environmental elements cannot be accurately judged in the prior art is solved, and accurate control over the cell transfection enrichment degree is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of cell transfection. Specifically, it relates to a method for controlling the enrichment degree of cell transfection based on the adjustment of environmental factors. Background Art

[0002] Cell transfection operations are currently playing a role in multiple fields. The core is to perform effective cell transfection operations. However, considering that cells have relatively strict requirements for the environment, the enrichment degree of cell transfection is low. Especially in the case of multiple transfection operations, metabolic wastes of cells, unreasonable adjustment of environmental factors, etc. will all lead to problems in cell transfection operations. In current cell transfection, for the adjustment of environmental factors, it is mainly to directly utilize the requirements of cells for the environment to adjust environmental factors. However, in this case, some parameters have a large parameter range, making it difficult to identify the precise requirements of cells for environmental factors, thereby improving the enrichment degree of cell transfection. In addition, for the enrichment degree of cell transfection, the density is only determined based on the environmental requirements of cell transfection, and then the density is directly equated with the cell enrichment degree. However, these two parameters are different in nature. In some cases, although the cell density is high, the transfection enrichment degree is low. This situation cannot be used for the application of transfected cells. That is to say, the prior art often confuses the concepts of cell density and enrichment degree during cell transfection and fails to comprehensively analyze the enrichment degree of cell transfection.

[0003] Therefore, how to improve the enrichment degree of cell transfection based on the adjustment of environmental factors is a technical problem that needs to be urgently solved by those skilled in the art. Summary of the Invention

[0004] In order to improve the enrichment degree of cell transfection based on the adjustment of environmental factors, the present application proposes a method for controlling the enrichment degree of cell transfection based on the adjustment of environmental factors, so as to obtain a method for adjusting environmental factors based on the specific analysis and determination of environmental factors and the determination of the enrichment degree. Specifically: A method for controlling the enrichment degree of cell transfection based on the adjustment of environmental factors, the control method includes: Based on the environmental requirements of cell transfection, establish multiple sets of environmental factor sets; Based on the environmental factor sets, perform cell transfection operations and record the execution time of the cell transfection operations; Obtain the cell density during the execution time of the cell transfection operation and establish a cell density curve; Based on the cell density curve, predict the cell density at the target termination time of cell transfection to obtain the theoretical transfected cell density; Obtain the actual transfected cell density based on the theoretical transfected cell density, and obtain the cell transfection enrichment degree corresponding to the actual transfected cell density; Obtain the ratio of the cell transfection enrichment degree to the actual transfected cell density to obtain the enrichment ratio; Obtain the set of environmental elements corresponding to the enrichment ratio to obtain a set of candidate environmental elements; Obtain the set of candidate environmental elements at different stages of cell transfection and establish a cell transfection environmental element chain.

[0005] Optionally, based on the environmental requirements for cell transfection, establish multiple sets of environmental element sets, including: Obtain the transfected cells and obtain the environmental requirements of the transfected cells; Based on the environmental requirements, set each environmental requirement parameter; Obtain the upper and lower limits of each environmental requirement parameter and set the optional values of each environmental requirement parameter; Establish a set of environmental elements, and each set of environmental elements contains all types of environmental elements; Establish multiple sets of environmental element sets, and in each set of environmental element sets, at least the optional values of one type of environmental element are different.

[0006] Optionally, based on the set of environmental elements, perform cell transfection operations and record the execution time of the cell transfection operations, including: Based on all the sets of environmental elements, construct a cell transfection operation environment; Based on the cell transfection operation environment, perform cell transfection operations; Obtain the cell transfection operation time to obtain the execution time of the cell transfection operation.

[0007] Optionally, obtain the cell density during the execution time of the cell transfection operation and establish a cell density curve, including: Obtain the end point of the execution time of the cell transfection operation to obtain the collection time point; Obtain the number of cells at the collection time point to obtain the cell density; Based on the cell density and the collection time point, establish a cell density curve; The establishment of the cell density curve further includes: Obtain the cell transfection operation time period, obtain the cell density in different cell transfection operation time periods to obtain the segmented cell density; Based on the segmented cell density and the cell transfection operation time period, establish a cell density curve.

[0008] Optionally, the cell density for predicting the target termination time of cell transfection based on the cell density curve is obtained to get the theoretical transfection cell density, including: Based on the cell density curve, obtain the cell density equation during the cell transfection process; Based on the cell density equation, obtain the cell density at the target termination time of the cell transfection to get the theoretical transfection cell density.

[0009] Optionally, it further includes: Obtain the cell transfection process and obtain the target termination time for each cell transfection process to get the segmented termination time; Obtain the environmental requirements for each cell transfection process, and select the set of environmental elements to get the preset set of environmental elements; Obtain the cell density at each segmented termination time under the culture environment of the preset set of environmental elements to get the measured transfection cell density; When the ratio of the theoretical transfection cell density to the measured transfection cell density is not lower than the preset ratio, obtain the preset set of environmental elements corresponding to the segmented termination time to get the available set of environmental elements.

[0010] Optionally, it is characterized in that obtaining the actual transfection cell density based on the theoretical transfection cell density and obtaining the cell transfection enrichment degree corresponding to the actual transfection cell density includes: Remove the theoretical transfection cell density when the ratio of the theoretical transfection cell density to the measured transfection cell density is lower than the preset ratio to obtain the actual transfection cell density; Remove the cell density where the actual transfection cell density is not higher than the preset actual transfection cell density to get the actual transfection cell density group; Obtain the transfected cell clusters corresponding to each actual cell transfection density in the actual transfection cell density group; Based on the transfected cell clusters, obtain the cell transfection enrichment degree.

[0011] Optionally, obtaining the ratio of the cell transfection enrichment degree to the actual transfection cell density to get the enrichment ratio includes: Obtain the ratio of the cell transfection enrichment degree to the actual transfection cell density to get the enrichment ratio group; Obtain all the enrichment ratios in the enrichment ratio group and remove the enrichment ratios where the enrichment ratio is not higher than the preset enrichment ratio to get the effective enrichment ratio.

[0012] Optionally, obtaining the set of environmental elements corresponding to the enrichment ratio and getting the set of candidate environmental elements includes: Based on the enrichment ratio, arrange the enrichment ratio to obtain an enrichment ratio sequence; Based on the enrichment ratio sequence, obtain a selected enrichment ratio; Obtain the set of environmental elements corresponding to the selected enrichment ratio to obtain a set of candidate environmental elements.

[0013] Optionally, the obtaining the set of candidate environmental elements at different stages of cell transfection and establishing a cell transfection environmental element chain includes: Based on the cell transfection process, obtain the cell transfection operation stage; Based on the cell transfection operation stage, obtain the corresponding set of candidate environmental elements; According to the cell transfection operation stage, associate the set of candidate environmental elements to establish a cell transfection environmental element chain.

[0014] The present application has the following beneficial effects: 1. It realizes the adjustment of environmental elements in multiple cell transfection operations. In the technical solution of the present application, it can cope with multiple cell transfection operations. Among them, by establishing an environmental element chain, in each cell transfection process, a corresponding set of environmental elements is established. Then, when reaching the corresponding cell transfection process, directly adjust according to the environmental element parameters in this set of environmental elements.

[0015] 2. It improves the adjustment accuracy of environmental elements. In the adjustment process of each environmental element in the present application, the cell transfection density and enrichment degree corresponding to the environmental element are analyzed, and the best set of environmental elements is selected from them, so as to ensure that under the environmental element situation, both the cell density and transfection enrichment degree meet the requirements, so as to fully improve the adjustment accuracy of environmental elements.

[0016] 3. It realizes the visualization of the cell transfection effect. In the technical solution of the present application, a cell density curve is established, and a cell transfection enrichment degree curve can be drawn on the basis of the cell density curve. In this way, the visualization processing of the cell transfection effect can be realized, and the information presentation level is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the embodiments of the present application or the prior art. Obviously, the following descriptions are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained according to these drawings. The drawings are used to provide a further understanding of the present disclosure and constitute a part of the specification, and are used together with the following specific embodiments to explain the present disclosure, but do not constitute a limitation to the present disclosure. In the drawings: Figure 1 Flow chart of a method for controlling cell transfection enrichment degree based on environmental factor adjustment provided by an embodiment of the present application; Figure 2 Cell density curve of a method for controlling cell transfection enrichment degree based on environmental adjustment disclosed by an embodiment of the present application; Figure 3 Schematic diagram of a cell transfection factor chain of a method for controlling cell transfection enrichment degree based on environmental factor adjustment provided by an embodiment of the present application. Detailed implementation manners

[0018] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the protection scope of the present application. In addition, in the embodiments of the present application, "first", "second", etc. are used to distinguish similar objects, and do not necessarily need to describe a specific order or sequence.

[0019] During cell transfection, especially in the case of multiple transfections, the requirements of cells for the environment are becoming more and more strict. If the environmental factors cannot be accurately adjusted, the enrichment degree of cell transfection is often low, and even cell death may occur, making transfection difficult. In current cell transfection operations, the main method is to determine the environmental requirements of cells and then set the environment. However, in many cases, the adjustment of environmental parameters is a relatively large range of values, which easily leads to a mismatch between the set environmental factors and the actual requirements during cell transfection, ultimately resulting in poor cell transfection effects, and although the transfection effect is good, the reproductive ability of cells is greatly restricted, making it difficult to further use the transfected cells.

[0020] The present application discloses a method for controlling cell transfection enrichment degree based on environmental factor adjustment in view of the above problems existing in the prior art. Specifically, as Figure 1 shown, it is a flow chart of a method for controlling cell transfection enrichment degree based on environmental factor adjustment disclosed by an embodiment of the present application. Specifically: S110. Based on the environmental requirements of cell transfection, establish multiple sets of environmental factor sets.

[0021] S120. Based on the environmental factor sets, perform cell transfection operations and record the execution time of the cell transfection operations.

[0022] S130. Obtain the cell density during the execution time of the cell transfection operations and establish a cell density curve.

[0023] S140. Based on the cell density curve, predict the cell density at the target termination time of cell transfection to obtain the theoretical transfected cell density.

[0024] S150. Obtain the actual transfected cell density based on the theoretical transfected cell density, and obtain the cell transfection enrichment degree corresponding to the actual transfected cell density.

[0025] S160. Obtain the ratio of the cell transfection enrichment degree to the actual transfected cell density to obtain the enrichment ratio.

[0026] S170. Obtain the set of environmental factors corresponding to the enrichment ratio to obtain the set of candidate environmental factors.

[0027] S180. Obtain the set of candidate environmental factors at different stages of cell transfection and establish an environmental factor chain for cell transfection.

[0028] The purpose of all the above steps is to adjust and set all the environmental factor parameters according to the cell's requirements for the environment during cell transfection, so as to establish multiple sets of environmental factor sets. Then, determine the specific effects of cell density and enrichment degree for the obtained environmental factor sets. In this case, confirm the best cell transfection effect, establish the corresponding environmental factor chain to obtain the best environmental factor data set, and set the cell transfection environment based on this data set to improve the cell transfection enrichment degree.

[0029] Next, specific explanations will be made for all the above technical features as follows: As described in step S110, the purpose of this step is that for cell transfection, especially for cells that require multiple transfection operations, different culture processes and transfection processes result in different requirements of cells for environmental factors during the transfection process. In order to accurately determine the specific requirements of cells for all environmental factors during cell transfection, it is necessary to establish a corresponding environmental requirement parameter data set for the environmental factor requirements that can be used during the cell transfection process, laying a foundation for subsequent environmental factor determination work. Specifically: S111. Obtain the transfected cells and obtain the environmental requirements of the transfected cells.

[0030] The purpose of this step is that in the determination of environmental factors during cell transfection, considering that although various other auxiliary substances, such as viruses, may be added, overall, the cell's requirements for environmental factors are the most important. Therefore, in the determination of environmental requirements, the environmental requirements of the transfected cells should be regarded as the highest requirement. Therefore, during the stage of determining environmental requirements, it is necessary to obtain the environmental requirements of the transfected cells.

[0031] Among them, based on the cell types used in the cell transfection process, the environmental requirements of this cell type during growth and cultivation are obtained.

[0032] Among them, in the specific process of cell treatment, it is also necessary to determine the environmental requirements of the cells at different growth stages during different growth processes of the cells, that is, during different time periods within the growth and / or different stages of growth of the cells.

[0033] Among them, within all the cell culture processes, it is also necessary to establish the correspondence between the cell culture process and the environmental requirements of the cells within this process, so as to establish the corresponding environmental element data group based on this correspondence.

[0034] Among them, for the obtained environmental requirements, this requirement only needs to be set based on the general environmental elements during the cell transfection process. That is to say, for the environmental requirements, they can be directly determined based on the existing environmental element requirement parameters of the same type of environmental elements currently, or the environmental requirements of a larger numerical range and a smaller number of cell transfection processes can be analyzed, and the obtained values are used as the environmental requirements of the transfected cells.

[0035] S112. Based on the said environmental requirements, set each environmental requirement parameter.

[0036] The purpose of this step is that when the environmental requirements are obtained, the types of environmental requirement parameters are obtained, and then based on the obtained types, the specific type parameters among them are set, so as to ensure that corresponding requirement parameters are set for each type of environmental requirement.

[0037] Among them, for the environmental requirements, it includes all contents that can affect the growth state of the transfected cells, such as environmental temperature, humidity, medium type, substances added to the medium, and the type and concentration of organisms, the concentration of toxic substances in the medium, etc., and these are used as the types of environmental requirements.

[0038] Among them, after determining the types of environmental requirements, obtain the environmental requirement parameters corresponding to all types of environmental requirements, and incorporate them into the environmental requirement data group.

[0039] Among them, for the obtained types of environmental requirements and environmental requirement parameters, it is necessary to establish an association relationship between the two. Then, in the determination of the environmental requirement parameters, after the subsequent establishment of the environmental requirement data group, the internal data of this data group is also established according to the correspondence relationship of environmental requirement type - environmental requirement parameter.

[0040] S113. Obtain the upper and lower limits of each environmental requirement parameter, and set the optional values of each environmental requirement parameter.

[0041] The purpose of this step is that after determining the upper and lower limits for each environmental requirement parameter, the parameter range to be set can be obtained. Then, based on this parameter range, a specific analysis can be made on the specific selectable contents of all the parameters therein to obtain the selectable values of various parameters.

[0042] Among them, for all the environmental requirement parameters that can be obtained, according to the data range that can be obtained therein, the environmental parameters can be randomly selected and used as selectable values.

[0043] In some embodiments, after determining the upper and lower limits of the environmental requirement parameters, a numerical selection step size is set for them. Then, based on this step size parameter, the selectable values of each environmental requirement parameter are obtained within the upper and lower limit range of the environmental requirement parameters.

[0044] Among them, for different types of environmental requirement parameters, the number of set selectable values can be different, and only need to be obtained based on the upper and lower limits of the relevant parameters.

[0045] S114. Establish an environmental element set, and each said environmental element set contains all environmental element types.

[0046] The purpose of this step is to establish an environmental set, which contains all environmental element types, and on the basis of this environmental element type, corresponding environmental element parameters are added to construct the corresponding environmental element set.

[0047] Among them, for the established environmental element set, a correspondence relationship needs to be established between the environmental element type and the environmental element parameter. Then, within the established data set, each environmental element parameter has a corresponding category correspondence relationship.

[0048] Among them, based on all the environmental element categories in the environmental element set, corresponding environmental element parameters are set for the corresponding categories to ensure that all parameters have their corresponding environmental element categories.

[0049] In some embodiments, according to the time period of the transfection operation, a corresponding environmental element set is established for each time period, and parameters need to be set for the environmental element types to be included in this set.

[0050] In some embodiments, it is also allowed that the set environmental element categories are different within different time periods of the transfection operation. That is to say, for the established environmental element set, it needs to be able to be completely determined based on the specific time stage of the environmental transfection process.

[0051] In some embodiments, an environmental factor set is established by first based on the occurrence time of the cell transfection stage, then determining the type of environmental requirements to be determined in the cell transfection stage, and finally adding environmental requirement parameters based on the type of environmental requirements.

[0052] S115. Establish multiple groups of environmental factor sets, and in each group of the environmental factor sets, at least one optional value of the environmental factor type is different.

[0053] The purpose of this step is that in the technical solution of the present application, in fact, in the establishment of the environmental factor set, a random numerical input method is used for processing, so it is very likely that in two or even more environmental factor sets, the environmental factor parameters and types are exactly the same. This step ensures that each environmental factor set is different by restricting the conditions.

[0054] Among them, for all the obtained environmental factor sets, the environmental factor parameters and categories therein are analyzed to determine all the environmental parameters.

[0055] Among them, it is necessary to ensure that in different environmental factor sets, at least one environmental factor type has different optional values in the two sets. That is to say, it is allowed that in two environmental factor sets, only the two optional values of one environmental factor type are the same, while all other types and parameters are the same.

[0056] As described in step S120, the purpose of this step is to perform a transfection operation on the cells, determine the cell transfection time, and based on the obtained execution time, obtain the density of the cells after transfection, so as to lay a foundation for subsequent environmental factor adjustment work. Specifically: S121. Based on all the environmental factor sets, construct a cell transfection operation environment.

[0057] The purpose of this step is to construct a cell transfection operation environment based on the obtained environmental factor sets, and then the current environmental factor set can be determined, and it can be determined whether the current environmental factor set is necessary and has a good transfection operation effect.

[0058] Among them, for the environmental factor set, which contains various environmental parameters, in specific processing, the cell transfection operation environment can be constructed based on the environmental parameters in the current environmental factor set.

[0059] Among them, for each environmental factor set, a corresponding environmental factor set label needs to be set in the corresponding environmental construction to establish the association between the current environmental factor set and the operation environment.

[0060] Among them, since the set of environmental factors needs to correspond to the corresponding cell transfection cycle, in the establishment of a specific operating environment, it is necessary to correspond to the cell transfection cycle corresponding to this set of environmental factors and also to the cell transfection cycle. For example, if a set of environmental factors corresponds to the initial stage of secondary transfection in cell transfection operations, the cells used must also be applied after one transfection, and a corresponding cell transfection operation environment is constructed.

[0061] S122. Perform cell transfection operations based on the cell transfection operation environment.

[0062] The purpose of this step is to, after establishing the cell transfection operation environment, perform cell transfection operations according to the correct operations to analyze the specific impact on cell transfection operations in the current operating environment.

[0063] Among them, based on all the constructed cell transfection operation environments, set the cells to be transfected therein, and then cell transfection operations can be performed within this cell transfection operation environment.

[0064] Among them, in cell transfection operations, it is necessary to completely perform corresponding cell transfection operations based on the corresponding cell transfection operation procedures.

[0065] S123. Obtain the cell transfection operation time to get the execution time of the cell transfection operation.

[0066] The purpose of this step is to, after determining the cell transfection operation time, establish a relationship curve between the cell transfection time and cell density during cell transfection to determine, within the specific cell transfection operation, based on this curve, obtain and / or predict the density of transfected cells in the actual cell transfection operation.

[0067] Among them, for cell transfection operations, the operation time can be decomposed into multiple time periods, and the density of cells can be determined at the end of each time period.

[0068] Among them, for the execution time of cell transfection operations, it can be directly used as the specific time period of cell transfection operations. That is to say, in the subsequent cell transfection density curve, each cell transfection operation time is used as a time parameter to obtain the execution time of cell transfection operations.

[0069] As described in step S130, the purpose of this step is to, by obtaining the cell density within each cell transfection operation execution time, then a correspondence between cell density and execution time can be established, thereby establishing a cell density curve, and then based on this density curve, obtain and / or predict the cell density that can be obtained in the actual cell transfection operation. Specifically: S131. Obtain the execution time endpoint of the cell transfection operation to obtain the collection time point.

[0070] Among them, for the cell transfection operation, multiple execution times of the transfection operation will be set. Obviously, each transfection operation time has a start and end time, and each end time point means the end of the culture period. Therefore, by obtaining the execution time endpoint and using it as the collection time point, the cell density within the execution period can be obtained.

[0071] Among them, obtain the execution time endpoint of each cell transfection operation, and then use this time endpoint as the collection time point.

[0072] In some embodiments, multiple execution time endpoints can also be directly set within a certain full-process time period and used as the collection time points.

[0073] S132. Obtain the number of cells at the collection time point and obtain the cell density.

[0074] The purpose of this step is that by obtaining the cell density, based on this cell density, the cell density that can be obtained in the current cell transfection operation environment can be obtained, so as to obtain whether the subsequent cell density meets the requirements of cell transfection.

[0075] Among them, for the acquisition of cell density, the existing cell density inspection method can be directly used for counting.

[0076] Among them, at each collection time point, obtain the cell density at this collection time point.

[0077] Among them, set the corresponding environmental factor set for each collection time point, then obtain the corresponding cell density, and establish a corresponding relationship between the two.

[0078] S133. Based on the cell density and the collection time point, establish a cell density curve.

[0079] The purpose of this step is that after obtaining the cell density and the collection time point, a corresponding cell density curve can be established. Then, in subsequent processing, based on this cell density curve, in the currently set environmental factor set, the environmental factor set can determine the cell density after the cell transfection operation, so as to realize the acquisition and / or prediction of the cell density.

[0080] Among them, after obtaining the cell density and the current collection time point, directly construct these two into the corresponding cell density curve to obtain the corresponding density curve result.

[0081] In some embodiments, the acquisition time point can also be directly replaced with the environmental factor set parameters. Thus, in this case, based on this environmental factor set, the result can be obtained.

[0082] Among them, as Figure 2 shown, it is the cell density curve of a cell transfection enrichment degree control method based on environmental adjustment disclosed in the embodiments of the present application. It is divided into four culture stages (a) to (d), namely the first stage, the second stage, the third stage, and the fourth stage. It can be found that at the ends of the first to third stages, the data is not coherent, indicating that these end regions may correspond to new culture stages or have reached the culture upper limit. Therefore, the data in the end regions is removed to avoid affecting the establishment of the density equation. In the fourth stage, after analyzing the parameters, it is found that the data is linearly related as a whole, and the mean value of all the values therein can be directly obtained. Of course, in many cases, the cell density may also have many other forms of density curves, and corresponding establishment is required for all of them.

[0083] Among them, in order to improve the representation accuracy of the cell density curve and under the decomposition method of the acquisition time point within the same execution time period, it is necessary to shorten the time length between adjacent two acquisition time points to obtain more cell density data.

[0084] Among them, in the case of combining multiple execution time periods, for the setting of adjacent acquisition time points, it is also necessary to shorten the time length between adjacent acquisition time points to the greatest extent under the condition of meeting the execution time length requirement to obtain more cell density data and improve the accuracy of the cell density curve.

[0085] Among them, the logical relationship between the acquisition time points is that the previous acquisition time point is the end point of the previous execution time period and at the same time the start point of the next execution time period.

[0086] Of course, in the case of the cell density within the execution time, there may be multiple methods that can be adopted, namely: one is the connection of the execution time periods of multiple cell transfections, and the other is the decomposition of the entire execution time period. In these two cases, corresponding methods need to be adopted for processing. Specifically: S134. The establishment of the cell density curve further includes: Obtain the cell transfection operation time period, obtain the cell density of different cell transfection operation time periods, and obtain the segmented cell density.

[0087] The purpose of this step is that for the cell density of all cell transfection operation time periods, it is necessary to set the operation time period within this time period to obtain the acquisition time point and obtain the segmented cell density corresponding to these different time periods.

[0088] Among them, for the cell transfection operation time period, it is necessary to determine the segmentation information of all cell transfection operation time periods therein. Then, the end time points of the execution of these segments need to be used as the collection time points, so as to obtain the cell density at these collection time points.

[0089] Among them, for the cell transfection operation time period within the cell transfection operation time period, it is necessary to obtain the cell density during the transfection operation time period based on the specific operation process of cell transfection.

[0090] Among them, it is necessary to ensure that each set cell transfection operation time period corresponds to the corresponding operation process, so as to set its segmented time nodes, so as to obtain the segmented cell density from the obtained setting result.

[0091] S135. Based on the segmented cell density and the cell transfection operation time period, establish a cell density curve.

[0092] The purpose of this step is to establish a cell density curve for these two parameters for all the obtained segmented cell densities and cell transfection operation time periods. Then, the cell density can be directly obtained and / or predicted based on this cell density curve.

[0093] Among them, for each segmented cell density, an association relationship is established with its corresponding cell transfection operation time period to obtain a cell density curve.

[0094] Among them, the method for establishing the cell density curve is the same as that in step S133, and will not be elaborated here.

[0095] As described in step S140, the purpose of this step is that after obtaining the cell density curve, considering that it obtains the corresponding cell density based on the already set collection time points, but in this method, the collection time points cannot be fully used to display the cell density during the whole process of cell transfection operation. Therefore, based on the cell density curve, the cell density that can be obtained during the actual cell transfection process is obtained. Specifically: S141. Based on the cell density curve, obtain the cell density equation of the cell transfection process.

[0096] The purpose of this step is to obtain the cell density equation of the cell transfection process based on the cell density curve, and this equation contains the cell transfection culture time and the cell density, so as to obtain the cell density equation and lay a foundation for the subsequent determination of the actual transfected cell density.

[0097] Among them, obtain the cell density curve and establish a specific equation based on this density curve.

[0098] Among them, in the obtained cell density equation, it is necessary to obtain the cell density equations for each time period of cell transfection and incorporate all the obtained cell density equations into the same system of equations.

[0099] Among them, for the obtained cell density equation, it is also necessary to determine the cell density equation for each transfection time period based on the acquisition time points in cell transfection.

[0100] Among them, in the transfection culture of a certain type of cell, based on each cell transfection process, it is necessary to obtain all the cell density equations, and the obtained equations are as follows: ; Among them, represents the cell density at the acquisition time point in the time period , A, B, C, d, D, E, f, G all represent the parameters in the corresponding cell density equation, H represents the cell density at the end point of the cell transfection operation time, represents the acquisition time point and the starting point of the transfection culture operation, represents the time period of the cell subculture work, i represents the index of the cell transfection operation cycle.

[0101] It should be noted that for each of these time nodes, they do not all need to be connected, because in some cases, a transfection culture is carried out using a time period shorter than the upper limit of the culture cycle, and then based on the cell density equation within this transfection time period, the cell density equation for this time period is obtained.

[0102] Among them, for the cell density in the time period, only a few time nodes need to be obtained, and when it is determined that the cell densities are the same, the cell density parameter can be directly determined as H That's it.

[0103] S142. Based on the cell density equation, obtain the cell density at the target termination time of the cell transfection to obtain the theoretical transfected cell density.

[0104] The purpose of this step is that in the case of obtaining the cell density equation, each density equation may not be at the final culture time node in the actual cell transfection culture. Therefore, in the specific processing, it is also necessary to obtain each complete culture time period to obtain the cell density at the end point of the corresponding culture time period.

[0105] Among them, obtain the density equation for each transfection operation time period, and then based on this density equation, obtain the cell density at the end point of the complete culture time period.

[0106] Among them, for the equations mentioned above, for the second culture cycle, the sampling time point is , but actually, the culture time length is , and it is found that , then the cell density is obtained based on the second cell density equation, but if , then the cell density is obtained based on the third cell density equation.

[0107] Among them, for each cell transfection operation time period, the corresponding cell density needs to be calculated, and the obtained cell density result is used as the actual transfected cell density.

[0108] After obtaining the cell density, considering that the cell density within each cell transfection operation time period is closely related to the corresponding culture environment, and some of these culture environments will have a negative impact on cell reproduction, such culture environments are obviously not applicable. At the same time, considering the establishment of a large number of environmental factor sets, therefore, whether from the perspective of directly removing the culture environment or from the perspective of reducing the workload of the subsequent environmental factor set screening process, it is necessary to remove the unusable environmental factor sets. Therefore, the present application also discloses the following technical solutions. Specifically: S143. Obtain the cell transfection process and obtain the target termination time of each said cell transfection process to obtain the segmented termination time.

[0109] The purpose of this step is to obtain each process in the cell transfection work, and then according to the obtained process information, obtain the start and end time points of each process, and determine the termination time within each process, so as to determine this termination time node, and thus the cell density corresponding to this time node can be obtained.

[0110] Among them, for the entire cell transfection process, all processes therein are determined, and the termination time node corresponding to each process is obtained, then this termination time node is directly used as the segmented termination time.

[0111] Among them, for the target termination time of each cell transfection process, it can be set by a technician, and the set target termination time is used as the segmented termination time.

[0112] In some embodiments, all the obtainable target termination times can be used as the specific culture cycles corresponding to the cell transfection process, such as: lag phase, logarithmic phase, stationary phase, etc., and the time end point of each cycle is obtained, and this time end point is the segmented termination time.

[0113] S144. Obtain the environmental requirements of each of the cell transfection processes, select the set of environmental elements, and obtain a preset set of environmental elements.

[0114] The purpose of this step is that since this application also needs to remove unreasonable culture environments based on cell density, but in cell transfection, the requirements for the culture environment are different for each step. Therefore, in this step, it is necessary to determine the environmental requirements of the cell transfection process, select a set of environmental elements that relatively meet the standards from them, and then shape the environment based on the selected set.

[0115] Among them, obtain each cell transfection process, and then select the set of environmental elements based on the requirements of the cells in this transfection process for the environment.

[0116] Among them, for the specific selection process of the set of environmental elements, compare each environmental parameter in the set of environmental elements with the requirement parameters of the cells for the environment. When it is found that each environmental parameter falls within the interval of the requirement parameters of the cells for the environment, then this set of environmental elements is considered a preset set of environmental elements.

[0117] Among them, when it is found that the parameters in a certain set of environmental elements have very small differences among all of them, but may be applied in multiple cell transfection processes, then this set of environmental elements can be applied in multiple cell transfection processes simultaneously.

[0118] S145. Obtain the cell density at each of the segment termination times under the culture environment of the preset set of environmental elements, and obtain the measured transfected cell density.

[0119] The purpose of this step is that in some cases, due to the existence of various factors, the cell density equation may have a high accuracy in a short period of time, but in a long period of time, it is found that there is a large difference between the actual cell density and the result obtained based on the density equation. For example, a certain environment may cause a large amount of metabolic waste to be produced by the cells, resulting in a large number of cell deaths or inability to further reproduce in a long period of time. Such an environment also needs to be removed. Therefore, it is necessary to obtain the measured cell density and verify the effect based on the obtained result.

[0120] Among them, when the termination time of the corresponding cell transfection process is reached, it is necessary to detect the cell density generated at this termination time. The specific measurement method is the same as the cell density measurement method in the above text, and will not be elaborated here.

[0121] S146. When the ratio of the theoretical transfected cell density to the measured transfected cell density is not lower than the preset ratio, obtain the preset set of environmental elements corresponding to the segment termination time, and obtain a set of available environmental elements.

[0122] The purpose of this step is that during cell transfection, especially in segmented transfection, it is affected by various factors. Therefore, for the comparison of the theoretical and actual densities of each segment, the deviation amount is obtained. If the deviation is too high, the transfection environment at this stage cannot meet the needs of the cells after transfection and needs to be excluded. Therefore, by setting comparison parameters, the set of available environmental factors is determined.

[0123] Among them, for the preset ratio, it can be set to 1. That is to say, as long as the ratio of the theoretical and measured transfected cell densities is not less than 1, the cell density equation corresponding to the theoretical transfected cell density can be considered available.

[0124] In some embodiments, the preset ratio can be directly set by a technician.

[0125] In some embodiments, the ratios of the theoretical and measured transfected cell densities in all cases of the set of environmental factors are obtained, and the mean value is calculated. The obtained mean value is the preset ratio.

[0126] As described in step S150, the purpose of this step is that since the ultimate goal of this application is to obtain a method for adjusting the environmental factors corresponding to the enrichment degree of cell transfection, it is necessary to determine the enrichment degree of cell transfection and select the set of environmental factors based on the obtained results. Specifically: S151. Remove the theoretical transfected cell density when the ratio of the theoretical transfected cell density to the measured transfected cell density is lower than the preset ratio, and obtain the actual transfected cell density.

[0127] The purpose of this step is that in all the above steps, the cell density obtained is essentially based on the density equation and belongs to the theoretical transfected cell density. However, in the control of the enrichment degree of cell transfection, the actual value needs to be used. In order to reduce the actual operation amount and better obtain the enrichment degree of cell transfection using the theoretical value, the available parameters in the theoretical transfected cell density need to be directly used as the actual transfected cell density and subsequent processing is carried out.

[0128] Among them, for the obtained theoretical transfected cell density, the ratio of the obtained theoretical value to the measured value needs to be calculated, and the theoretical values lower than the preset ratio are removed. The remaining theoretical values are the actual transfected cell density.

[0129] In some embodiments, for the obtained measured transfected cell density, since the difference between it and the theoretical value is small, the obtained measured transfected cell density can be directly used as the actual transfected cell density.

[0130] Among them, the obtained actual transfected cell density is all obtained from the theoretical transfected cell density.

[0131] S152. Remove the cell density where the actual transfection cell density is not higher than the preset actual transfection cell density to obtain an actual transfection cell density group.

[0132] The purpose of this step is that for the obtained actual transfection cell density, all the values therein need to be included in the actual transfection cell density group, and then the availability of the actual transfection cell density in this data group can be judged, and the corresponding environmental factor set can be further removed based on the cell density.

[0133] Among them, all the actual transfection cell densities are included in the same data group to obtain an actual transfection cell density group.

[0134] In some embodiments, for all the actual transfection cell densities in the actual transfection cell density group, corresponding environmental factor sets are set, so that the actual transfection cell density and the corresponding environmental factor sets are both set in the actual transfection cell density group.

[0135] S153. Obtain the transfected cell clusters corresponding to each actual cell transfection density in the actual transfection cell density group.

[0136] The purpose of this step is that after obtaining the actual transfection cell density group, which contains multiple actual transfection cell density data, and each actual transfection cell density data corresponds to an environmental factor set. After setting the transfection environment based on this set and then culturing cells based on this transfection environment, cell clusters can be obtained. After obtaining the cell clusters, the cell transfection enrichment degree can be obtained.

[0137] Among them, obtain the environmental factor set corresponding to each actual transfection cell density, and construct a transfection environment based on this environmental factor set.

[0138] Among them, after constructing the cell transfection environment, cell transfection culture will be carried out in this transfection environment, and cell clusters will be obtained during the corresponding culture time period.

[0139] Among them, the cell cluster refers to the aggregate of all transfected cells present in the culture medium when cells are transfected and cultured.

[0140] S154. Obtain the cell transfection enrichment degree based on the transfected cell clusters.

[0141] The purpose of this step is that after obtaining the cell clusters, the cell transfection enrichment degree can be obtained by measuring all the cells in this cell cluster.

[0142] Among them, after obtaining the cell clusters, relevant equipment is used to measure the cell transfection enrichment degree.

[0143] Among them, for the cell transfection enrichment degree, it is necessary to establish the corresponding relationship between the cell transfection enrichment degree and the corresponding set of environmental factors, so as to obtain the corresponding relationship between the two types of data.

[0144] In some embodiments, based on all sets of environmental factors, the cell transfection enrichment degree in its transfection culture environment is measured in full.

[0145] Among them, it is also necessary to obtain the cell transfection enrichment degree corresponding to the cell transfection result in each cell transfection process based on the cell transfection process. That is to say, obtain the cell transfection enrichment degree in each cell transfection process, and establish a corresponding relationship between each cell transfection enrichment degree and the set of environmental factors of the corresponding cell transfection process.

[0146] As described in step S160, the purpose of this step is to judge the cell enrichment degree of the cell cluster under the condition that the cell density in the cell transfection operation has been obtained and the cell density meets the index, so as to further select the environmental factors to be established based on the cell enrichment degree. Specifically: S161. Obtain the ratio of the cell transfection enrichment degree to the actual transfected cell density to obtain an enrichment ratio group.

[0147] The purpose of this step is to specifically screen the cell transfection enrichment degree, and then determine the cell transfection enrichment degree in all cell transfection operations that meet the requirements. After obtaining the determination result, the environmental factor set can be further screened based on the enrichment degree data in this enrichment ratio group.

[0148] Among them, obtain the ratio of the cell transfection enrichment degree to the actual transfected cell density, so as to obtain the ratio of the cell transfection enrichment degree under the condition of the actual transfected cell density.

[0149] Among them, compare the obtained ratio of the cell transfection enrichment degree with a preset ratio. Only when it exceeds the preset ratio can it be further used. For the convenience of description, the ratio of the cell transfection enrichment degree is named "enrichment degree ratio".

[0150] Among them, all parameters with an enrichment degree ratio not lower than the preset enrichment degree ratio are considered to be the actual cell transfection enrichment degree or density that can be further used. Then, the cell cluster corresponding to the actual transfected cell density and the set of environmental factors corresponding to the cell cluster can be used in the next step, that is: this set of environmental factors is the set of environmental factors that meet the application standards.

[0151] Among them, set the data with the obtained enrichment degree ratio meeting the standard directly into the enrichment ratio group. The enrichment ratio group is actually a data group.

[0152] Among them, in the enrichment ratio group, a corresponding relationship is established between the enrichment degree ratio and the set of environmental elements and stored.

[0153] Among them, for the obtained enrichment ratio group, all the corresponding relationships therein need to be stored before the enrichment ratio group can be obtained.

[0154] S162. Obtain all the enrichment ratios in the enrichment ratio group, and remove the enrichment ratios whose enrichment ratios are not higher than the preset enrichment ratio to obtain effective enrichment ratios.

[0155] The purpose of this step is to further judge the available enrichment ratios based on the cell transfection enrichment degree, so as to further judge the environmental elements in the currently determined cell transfection that can be used, and thus remove the set of environmental elements that do not meet the requirements.

[0156] Among them, the enrichment ratio of the preset enrichment ratio for the enrichment degree ratio can be determined based on the experience of technicians.

[0157] In some embodiments, the mean value of all the obtained enrichment ratios is calculated, and the obtained mean value is directly determined as the enrichment ratio of the preset enrichment ratio.

[0158] Among them, only the parameter of the enrichment ratio whose obtained enrichment ratio is higher than the preset enrichment ratio is the enrichment ratio.

[0159] As described in step S170, the purpose of this step is to screen the set of environmental elements that completely meet the requirements, so as to determine the set of environmental elements that can be used in each culture process in the control of cell transfection enrichment degree. Specifically: S171. Arrange the enrichment ratios based on the enrichment ratios to obtain an enrichment ratio sequence.

[0160] The purpose of this step is that for each cell transfection operation process, it is often found that there may be multiple sets of environmental elements on this process, and each set of environmental elements will have a corresponding enrichment ratio. In order to obtain better technical effects, it is necessary to use the set of environmental elements with a higher enrichment ratio, and then use the obtained best set of environmental elements.

[0161] Among them, all the obtained enrichment ratios are determined and sorted according to the specific parameters of the enrichment ratios.

[0162] Among them, for the obtained enrichment ratios, they can be sorted in descending or ascending order, and the specific sorting method is not limited in this application.

[0163] S172. Obtain a selected enrichment ratio based on the enrichment ratio sequence.

[0164] The purpose of this step is that after obtaining the enrichment ratio sequence, based on this enrichment ratio sequence, further screening of the enrichment ratio is performed. That is to say, after multiple steps of selection of the enrichment ratio, this selection process is determined based on the set hard requirements, and then further determination is required to achieve determination based on soft requirements.

[0165] Among them, for the acquisition of the selected enrichment ratio, it is necessary to be able to directly set the number of selection parameters in each enrichment ratio sequence to select the corresponding enrichment ratio, which is the selected enrichment ratio.

[0166] In some embodiments, in order to reduce the cultivation difficulty of cell transfection enrichment, for the obtained selected enrichment ratio, directly select the enrichment ratio with the largest enrichment ratio value in the enrichment ratio sequence.

[0167] S173. Obtain the set of environmental elements corresponding to the selected enrichment ratio to obtain a set of candidate environmental elements.

[0168] The purpose of this step is that after obtaining the selected enrichment ratio, this parameter corresponds to the cell transfection process. Therefore, in the process, based on the obtained selected enrichment ratio, the corresponding set of environmental elements can be obtained. Obviously, this set of environmental elements is the set of environmental elements that can obtain the maximum cell transfection enrichment degree in cell transfection.

[0169] Among them, based on the corresponding relationship between the set of environmental elements recorded above and other various parameters, the corresponding relationship between the selected enrichment ratio and the set of environmental elements is obtained, so as to obtain the set of environmental elements based on the selected enrichment ratio, and thus obtain the set of candidate environmental elements.

[0170] In some embodiments, for all the obtained enrichment ratios, a corresponding relationship is established with their corresponding sets of environmental elements. Then, after obtaining the selected enrichment ratio, the set of candidate environmental elements can be directly obtained based on this corresponding relationship.

[0171] As described in step S180, the purpose of this step is that after obtaining all the sets of candidate environmental elements, since the ultimate goal of this application is to obtain a good cell transfection enrichment degree, and all the sets of candidate environmental elements are the sets of environmental elements that can obtain the best cell transfection enrichment degree in the corresponding cell transfection process, constructing the obtained sets of environmental elements according to the cell transfection process can obtain the best cell transfection environmental element chain in the cell transfection process. Specifically: S181. Obtain the cell transfection operation stage based on the cell transfection process.

[0172] The purpose of this step is that in the construction of the cell transfection environmental factor chain, it is obvious that all cell transfection process information in the cell transfection process needs to be processed. Thus, in this case, an association can be established between the set of candidate environmental factors and the corresponding cell transfection process. Therefore, it is necessary to first obtain the stages of cell transfection operations.

[0173] Among them, for all the obtained sets of candidate environmental factors, the corresponding relationship between this set and the cell transfection process needs to be obtained to get different stages of cell transfection.

[0174] Among them, the situation that may occur is that for the same cell transfection process, there are multiple different sets of candidate environmental factors. In this case, it is necessary to ensure that only one set of candidate environmental factors is selected in this stage, and this stage is identified as the cell transfection stage.

[0175] Among them, for all the obtained cell transfection working stages, it is necessary to ensure that there are no identical culture stages among the corresponding different stages of cell transfection obtained.

[0176] S182. Based on the cell transfection operation stage, obtain the corresponding set of candidate environmental factors.

[0177] The purpose of this step is that after obtaining the cell transfection operation stage, in fact, for all cell transfection operation stages, sets of environmental factors are set, and the same transfection operation stage corresponds to multiple sets of environmental factors. Then, the set of candidate environmental factors is determined from the obtained sets of environmental factors. At this time, based on the cell transfection operation stage, the corresponding set of candidate environmental factors can also be obtained, so as to obtain the construction factors of the cell transfection environmental factor chain.

[0178] Among them, within each cell transfection operation stage, multiple different sets of environmental factors are set based on the environmental parameter range. That is to say, the corresponding relationship between the cell transfection operation stage and the set of environmental factors is established.

[0179] Among them, the obtained set of candidate environmental factors is obtained from multiple sets of environmental factors corresponding to the same cell transfection operation stage. Therefore, the set of candidate environmental factors and the cell transfection operation process itself have a corresponding relationship.

[0180] S183. According to the different stages of cell transfection, associate the sets of candidate environmental factors to establish a cell transfection environmental factor chain.

[0181] The purpose of this step is that for all the obtained candidate environmental factor sets and based on the correspondence between the candidate environmental factor sets and the cell transfection operation stages, the corresponding candidate environmental factor sets can be directly associated based on the cell transfection operation stages, thereby obtaining the cell transfection environmental factor chain.

[0182] Among them, for the cell transfection process, all the cell transfection operation stages are obtained therein. That is to say, after each cell transfection operation stage is sorted in order, the complete cell transfection process is obtained.

[0183] Among them, after obtaining the cell transfection operation stages, it is necessary to sort according to the cell transfection process, and then associate the environmental factor sets corresponding to each process, thereby obtaining the cell transfection environmental factor chain.

[0184] Among them, when multiple candidate environmental factor sets correspond to the same cell transfection operation stage, it is necessary to establish an association relationship between each factor in all the candidate environmental factor sets corresponding to the previous cell transfection process and all the selected environmental factor sets corresponding to the next cell transfection process. As Figure 3 shown, it is a schematic diagram of the cell transfection factor chain of a cell transfection enrichment degree control method based on environmental factor adjustment provided by an embodiment of the present application. Among them, for the established cell transfection factor chain, there may be multiple candidate environmental factor sets included in each transfection culture stage. One is selected in each stage and connected to the candidate environmental factor sets of other stages, thereby forming a complete cell transfection factor chain, such as: A1 - B2 - C2 - D.

[0185] The present application includes the following beneficial effects: 1. The adjustment of environmental factors in multiple cell transfection operations is realized. In the technical solution of the present application, it can cope with multiple cell transfection operations. Among them, by establishing an environmental factor chain, a corresponding environmental factor group is established within each cell transfection process. When reaching the corresponding cell transfection process, it can be directly adjusted according to the environmental factor parameters in this environmental factor group.

[0186] 2. The adjustment accuracy of environmental factors is improved. In the adjustment process of each environmental factor in the present application, the cell transfection density and enrichment degree corresponding to the environmental factor are analyzed, and the best environmental factor group is screened out, so as to ensure that both the cell density and transfection enrichment degree meet the requirements in the environmental factor context, so as to fully improve the adjustment accuracy of environmental factors.

[0187] 3. Visualize the cell transfection effect. In the technical solution of this application, a cell density curve is established, and a cell transfection enrichment curve can be drawn based on the cell density curve. In this way, the visualization process of the cell transfection effect can be achieved, improving the information presentation level.

[0188] Those of ordinary skill in the art can understand that all or part of the steps of implementing the above method embodiments can be completed by hardware related to computer program instructions. The foregoing computer program can be stored in a non-volatile storage medium. When the computer program is executed, it executes the steps including the above method embodiments. Alternatively, if the above integrated unit is implemented in the form of a software functional module and sold or used as an independent product, it can also be stored in a non-volatile storage medium. Based on such an understanding, the technical solution of the embodiments of the present invention, in essence, or the part that contributes to the prior art, can be embodied in the form of a software product. The computer software product is stored in a non-volatile storage medium and includes several instructions for causing an electronic device (which can be a personal computer, a server, a network device, etc.) to execute all or part of the methods described in the various embodiments of the present invention.

[0189] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered by the protection scope of the present invention.

Claims

1. A method for controlling the enrichment degree of cell transfection based on environmental factor adjustment, characterized in that, The control method includes: Based on the environmental requirements of cell transfection, establish multiple sets of environmental factor sets; Based on the environmental factor sets, perform cell transfection operations and record the execution time of the cell transfection operations; Obtain the cell density during the execution time of the cell transfection operations and establish a cell density curve; Based on the cell density curve, predict the cell density at the target termination time of cell transfection to obtain the theoretical transfected cell density; Based on the theoretical transfected cell density, obtain the actual transfected cell density and obtain the cell transfection enrichment degree corresponding to the actual transfected cell density; Obtain the ratio of the cell transfection enrichment degree to the actual transfected cell density to obtain the enrichment ratio; Obtain the environmental factor set corresponding to the enrichment ratio to obtain the candidate environmental factor set; Obtain the candidate environmental factor sets at different stages of cell transfection and establish a cell transfection environmental factor chain.

2. The method for controlling the enrichment degree of cell transfection adjusted based on environmental factors according to claim 1, wherein The establishing multiple sets of environmental factor sets based on the environmental requirements of cell transfection includes: Obtain the cells to be transfected and obtain the environmental requirements of the cells to be transfected; Based on the environmental requirements, set each environmental requirement parameter; Obtain the upper and lower limits of each environmental requirement parameter and set the optional values of each environmental requirement parameter; Establish an environmental factor set, and each environmental factor set contains all the environmental factor types; Establish multiple sets of environmental factor sets, and in each set of environmental factor sets, at least one of the optional values of the environmental factor types is different.

3. The method for controlling the enrichment degree of cell transfection based on environmental factor adjustment according to claim 1, wherein The performing cell transfection operations based on the environmental factor sets and recording the execution time of the cell transfection operations includes: Based on all the environmental factor sets, construct a cell transfection operation environment; Based on the cell transfection operation environment, perform cell transfection operations; Obtain the cell transfection operation time to obtain the execution time of the cell transfection operations.

4. The method for controlling the enrichment degree of cell transfection based on environmental factor adjustment according to claim 1, wherein, The obtaining the cell density during the execution time of the cell transfection operations and establishing a cell density curve includes: Obtain the end point of the execution time of the cell transfection operations to obtain the collection time point; Obtain the cell number at the collection time point and obtain the cell density; Based on the cell density and the collection time point, establish a cell density curve; The establishing a cell density curve further includes: Obtain the cell transfection operation time period, obtain the cell density at different cell transfection operation time periods to obtain the segmented cell density; Based on the segmented cell density and the cell transfection operation time period, establish a cell density curve.

5. The method for controlling the enrichment degree of cell transfection based on environmental factors according to claim 1, wherein The predicting the cell density at the target termination time of cell transfection based on the cell density curve to obtain the theoretical transfected cell density includes: Based on the cell density curve, obtain the cell density equation of the cell transfection process; Based on the cell density equation, obtain the cell density at the target termination time of cell transfection to obtain the theoretical transfected cell density.

6. The method for controlling the enrichment degree of cell transfection based on environmental factors according to claim 5, wherein It further includes: Obtain the cell transfection process and obtain the target termination time of each cell transfection process to obtain the segmented termination time; Obtain the environmental requirements of each cell transfection process and select the environmental factor set to obtain the preset environmental factor set; Under the condition of obtaining the culture environment of the preset environmental factor set, the cell density at each of the segment termination times is obtained to get the measured transfected cell density; When the ratio of the theoretical transfected cell density to the measured transfected cell density is not lower than a preset ratio, the preset environmental factor set corresponding to the segment termination time is obtained to get the available environmental factor set.

7. The method for controlling the enrichment degree of cell transfection adjusted based on environmental factors according to any one of claims 1 to 6, characterized in that, The obtaining of the actual transfected cell density based on the theoretical transfected cell density and the obtaining of the cell transfection enrichment degree corresponding to the actual transfected cell density include: The theoretical transfected cell density when the ratio of the theoretical transfected cell density to the measured transfected cell density is lower than the preset ratio is removed to obtain the actual transfected cell density; The cell densities with the actual transfected cell density not higher than the preset actual transfected cell density are removed to obtain a group of actual transfected cell densities; For each actual cell transfection density in the group of actual transfected cell densities, the transfected cell cluster corresponding thereto is obtained; Based on the transfected cell cluster, the cell transfection enrichment degree is obtained.

8. The method for controlling the enrichment degree of cell transfection adjusted based on environmental factors according to claim 1, wherein The obtaining of the ratio of the cell transfection enrichment degree to the actual transfected cell density to get the enrichment ratio includes: The ratio of the cell transfection enrichment degree to the actual transfected cell density is obtained to get a group of enrichment ratios; All the enrichment ratios in the group of enrichment ratios are obtained, and the enrichment ratios with the enrichment ratio not higher than the preset enrichment ratio are removed to obtain effective enrichment ratios.

9. The method for controlling the enrichment degree of cell transfection adjusted based on environmental factors according to claim 1, wherein The obtaining of the environmental factor set corresponding to the enrichment ratio and getting the candidate environmental factor set includes: Based on the enrichment ratio, the enrichment ratios are arranged to get an enrichment ratio sequence; Based on the enrichment ratio sequence, a selected enrichment ratio is obtained; The environmental factor set corresponding to the selected enrichment ratio is obtained to get the candidate environmental factor set.

10. The method for controlling the enrichment degree of cell transfection adjusted based on environmental factors according to claim 1, wherein The obtaining of the candidate environmental factor sets at different stages of cell transfection and establishing a cell transfection environmental factor chain includes: Based on the cell transfection process, the cell transfection operation stages are obtained; Based on the cell transfection operation stages, the corresponding candidate environmental factor sets are obtained; According to the cell transfection operation stages, the candidate environmental factor sets are associated to establish a cell transfection environmental factor chain.

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