Anti-oxidation composition based on reverse micelle synergistic technology as well as preparation method and application of anti-oxidation composition

Abstract

The invention relates to an anti-oxidation composition based on a reverse micelle synergistic technology and a preparation method and application thereof, the anti-oxidation composition comprises a reverse micelle structure and an aqueous solution containing an anti-oxidation active matter, and the reverse micelle structure wraps the aqueous solution containing the anti-oxidation active matter. The reverse micelle structure is used for wrapping the water-soluble antioxidant active matter, oxidation or degradation discoloration of the water-soluble antioxidant active matter is prevented, after the antioxidant active matter such as hydroxytyrosol, ergothioneine and dipotassium glycyrrhizinate is wrapped by the reverse micelle, the stability and the effect are remarkably improved, and after the reverse micelle wrapping, the effect of the antioxidant active matter is improved by 17% or above compared with an aqueous solution before wrapping.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an antioxidant composition based on reverse micelle synergistic technology, a preparation method thereof, and an application thereof. Background Art

[0002] Antioxidant technology has important applications in the field of cosmetics. Common antioxidant actives are restricted in their efficacy due to problems such as easy oxidation, poor stability, and low efficacy efficiency. In the prior art, antioxidant actives are usually directly added in the form of an aqueous solution, or encapsulated through carriers such as liposomes and microemulsions to improve stability and permeability.

[0003] CN111035579A discloses a composite liposome / chitosan antioxidant preparation and a preparation method thereof. Phospholipids, cholesterol, and the antioxidant active caffeic acid are dissolved in an organic solvent to form an oil phase; an aqueous phase containing glutathione is added in two portions, and a water / oil / water three-phase system is formed by high-speed stirring, and the organic solvent is removed to obtain primary liposomes; after ultrasonic treatment, an acetic acid solution containing chitosan is added to obtain a composite liposome antioxidant preparation. By encapsulating antioxidant actives (such as caffeic acid and glutathione) in liposomes, their stability and solubility are improved.

[0004] The journal "Microemulsion-based delivery systems for antioxidants: Enhanced stability and bioavailability of coenzyme Q10 and green tea extract" in Journal of Controlled Release, Volume 220, Issue Part A, 2015, Pages 141-150 studied a microemulsion-based antioxidant active delivery system. A surfactant, a co-surfactant, and an oil phase are mixed to form a microemulsion system; the antioxidant active is dissolved in the aqueous phase and slowly added to the microemulsion system; a stable microemulsion is formed by high-speed homogenization. By encapsulating antioxidant actives (such as coenzyme Q10 and green tea extract) in microemulsions, their stability and bioavailability are improved.

[0005] Although the above technologies have improved the stability of antioxidant actives to a certain extent, the following problems still exist: 1) Liposome technology: The preparation process is complicated, requiring rotary evaporation and ultrasonic treatment, which is difficult to scale up; liposomes have poor stability and are prone to oxidation or leakage, resulting in the ineffectiveness of actives. 2) Microemulsion technology: A large amount of surfactants and co-surfactants are required, which may cause irritation to the skin; The stability of the microemulsion system is greatly affected by factors such as temperature and pH value, and its scope of application is limited. 3) Direct addition of aqueous solution: The transdermal absorption rate is low, the active ingredients are easily degraded, and the antioxidant effect is limited.

[0006] In the prior art, the carrier systems of antioxidant active ingredients generally have the following problems: 1) Insufficient stability: the active ingredients are easily oxidized or degraded, resulting in reduced efficacy; 2) Complex preparation process: the existing carrier systems (such as liposomes, microemulsions) have harsh preparation conditions and are difficult to mass produce; 3) Skin irritation: some carrier systems require the use of a large amount of surfactants, which may cause irritation to the skin.

[0007] In summary, providing a method for improving the stability of antioxidant active substances has become one of the problems to be solved urgently in the art. Summary of the invention

[0008] In order to solve the above technical problems, the present invention provides an antioxidant composition based on reverse micelle enhancement technology, and a preparation method and application thereof. By encapsulating antioxidant active substances in reverse micelles, the problems of poor stability, low onset efficiency, and complex preparation process of antioxidant active substances in the prior art are solved, and the stability and efficacy of antioxidant active substances are significantly improved.

[0009] To achieve this object, the present invention adopts the following technical solutions:

[0010] In a first aspect, the present invention provides an antioxidant composition based on reverse micelle enhancement technology, wherein the antioxidant composition comprises a reverse micelle structure and an aqueous solution containing antioxidant active substances, wherein the reverse micelle structure encapsulates the aqueous solution containing antioxidant active substances.

[0011] The present invention uses a reverse micelle structure to encapsulate a water-soluble antioxidant active substance, thereby improving the stability of the antioxidant active substance and preventing the antioxidant active substance from being oxidized or degraded and discolored.

[0012] Preferably, the raw materials for preparing the reverse micelle structure include phospholipids, non-polar solvents and antioxidants.

[0013] In the present invention, phospholipids are the main structural components of reverse micelles and provide a stable encapsulation system. Non-polar solvents help to form reverse micelle structures, and antioxidants are used to improve the stability of the composition.

[0014] Preferably, the purity of the phospholipid is 90% to 99% (such as 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, etc.).

[0015] Preferably, the non-polar solvent includes any one or a combination of at least two of isopropyl palmitate, isopropyl myristate or isopropyl laurate.

[0016] Preferably, the antioxidant includes any one or a combination of at least two of butylated hydroxyanisole, butylated hydroxytoluene, tert-butyl hydroquinone or tocopheryl acetate.

[0017] Preferably, the raw materials for preparing the reverse micelle structure include 20 to 50 (such as 20, 25, 30, 35, 40, 45 or 50, etc.) parts by weight of phospholipid, 45 to 78 (such as 45, 50, 55, 60, 65, 70, 75 or 78, etc.) parts by weight of non-polar solvent and 1 to 2 (such as 1, 1.2, 1.4, 1.5, 1.6, 1.8 or 2, etc.) parts by weight of antioxidant.

[0018] Preferably, the antioxidant active substances in the aqueous solution containing antioxidant active substances include any one or a combination of at least two of hydroxytyrosol, ergothioneine or dipotassium glycyrrhizinate.

[0019] Through the selection of antioxidant active substances, the present invention is compounded with the antioxidants in the reverse micelle structure, synergistically enhancing the antioxidant ability of the antioxidant composition based on the reverse micelle synergistic technology provided by the present invention. The combination of hydroxytyrosol and tocopheryl acetate is preferred.

[0020] Preferably, the mass percentage content of the antioxidant active substances in the aqueous solution containing antioxidant active substances is 1% to 50% (such as 1%, 2%, 5%, 10%, 20%, 30%, 40% or 50%, etc.).

[0021] Preferably, the raw materials for preparing the antioxidant composition include phospholipid, non-polar solvent, antioxidant and aqueous solution containing antioxidant active substances.

[0022] Preferably, the raw materials for preparing the antioxidant composition include 20 to 50 (such as 20, 25, 30, 35, 40, 45 or 50, etc.) parts by weight of phospholipid, 45 to 78 (such as 45, 50, 55, 60, 65, 70, 75 or 78, etc.) parts by weight of non-polar solvent, 1 to 2 (such as 1, 1.2, 1.4, 1.5, 1.6, 1.8 or 2, etc.) parts by weight of antioxidant and 1 to 3 (such as 1, 1.5, 1.8, 2, 2.2, 2.5 or 3, etc.) parts by weight of aqueous solution containing antioxidant active substances.

[0023] In a second aspect, the present invention provides a method for preparing the antioxidant composition as described in the first aspect, and the preparation method includes the following steps:

[0024] (1) Dissolve phospholipids, a non-polar solvent, and an antioxidant to form a uniform oil phase, and cool while stirring;

[0025] (2) Prepare an aqueous solution containing an antioxidant-active substance as the aqueous phase;

[0026] (3) Add the aqueous phase to the oil phase while stirring to obtain the antioxidant composition.

[0027] Preferably, the temperature of the dissolution in step (1) is 70 - 90 °C (for example, it can be 70 °C, 75 °C, 78 °C, 80 °C, 82 °C, 85 °C, or 90 °C, etc.).

[0028] Preferably, the stirring speed in step (1) is 50 - 150 rpm (for example, it can be 50 rpm, 70 rpm, 90 rpm, 100 rpm, 110 rpm, 130 rpm, or 150 rpm, etc.).

[0029] Preferably, the final temperature of the cooling in step (1) is 20 - 30 °C (for example, it can be 20 °C, 22 °C, 24 °C, 25 °C, 26 °C, 28 °C, or 30 °C, etc.).

[0030] Preferably, the stirring speed in step (3) is 250 - 350 rpm (for example, it can be 250 rpm, 270 rpm, 290 rpm, 300 rpm, 310 rpm, 330 rpm, or 350 rpm, etc.).

[0031] The present invention ensures the uniformity and stability of the reverse micelle structure by setting a specific range of stirring speeds for the oil phase and the aqueous phase.

[0032] Preferably, the amount added each time in the added aqueous phase in step (3) is 10 - 30 μL (for example, it can be 10 μL, 15 μL, 18 μL, 20 μL, 22 μL, 25 μL, or 30 μL, etc.).

[0033] In a third aspect, the present invention provides the use of the antioxidant composition as described in the first aspect in the preparation of cosmetics.

[0034] Preferably, the cosmetics include any one or a combination of at least two of emulsion, lotion, eye cream, essence milk, cream, or facial mask.

[0035] Other specific point values within the above numerical ranges can be selected, and they will not be elaborated one by one here.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] (1) Through the self-assembly of phospholipids in polar and non-polar solvents, the present invention forms a stable reverse micelle structure, which effectively encapsulates water-soluble antioxidant active substances, preventing their oxidation, degradation, or color change. After being encapsulated by reverse micelles, the stability and efficacy of antioxidant active substances such as hydroxytyrosol, ergothioneine, and dipotassium glycyrrhizinate are significantly improved. After being encapsulated by reverse micelles, the efficacy of antioxidant active substances is increased by more than 17% compared to the aqueous solution before encapsulation. The antioxidant used in the preparation of the reverse micelle structure of the present invention and the antioxidant active substances encapsulated in the reverse micelle structure have a synergistic effect, jointly enhancing the antioxidant effect of the product.

[0038] (2) The reverse micelle preparation method provided by the present invention is simple to operate and easy to scale up production. The present invention also optimizes the preparation process of the reverse micelle structure, setting a specific stirring speed range for the oil phase and water phase to ensure the uniformity and stability of the reverse micelle structure.

[0039] (3) The present invention does not require the use of a large amount of surfactants in the preparation of the composition. The obtained product is mild and non-irritating to the skin, and can also be applied to encapsulate a variety of water-soluble antioxidant active substances, having a wide range of application prospects. Description of the Drawings

[0040] Figure 1 It is the transmission electron microscopy observation result diagram in Test Example 1.

[0041] Figure 2 It is the color change result diagram of hydroxytyrosol after 120 days of constant temperature treatment at 45°C in Test Example 3. Detailed Embodiments

[0042] To further illustrate the technical means and effects adopted by the present invention, the present invention will be further described below in conjunction with examples and drawings. It can be understood that the specific embodiments described herein are only used to explain the present invention, rather than limiting the present invention.

[0043] For those not specifying specific techniques or conditions in the examples, they shall be carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. For reagents or instruments not indicating the manufacturer, they are all conventional products that can be obtained through regular channels.

[0044] Preparation Example 1

[0045] This preparation example provides a preparation method of an antioxidant composition based on reverse micelle synergistic technology, including the following steps:

[0046] (1) Dissolve phospholipids, non-polar solvents, and antioxidants at 80°C to form a uniform oil phase, stir at a speed of 100 rpm, and cool while stirring until it reaches 25°C;

[0047] (2) Prepare an aqueous solution containing antioxidant active substances as the aqueous phase;

[0048] (3) Add the aqueous phase to the oil phase with stirring at a speed of 300 rpm, and the addition amount each time is 20 μL to obtain the antioxidant composition.

[0049] Preparation Example 2

[0050] This preparation example provides a preparation method of an antioxidant composition based on reverse micelle synergistic technology, including the following steps:

[0051] (1) Dissolve phospholipids, non-polar solvents and antioxidants at 70 °C to form a uniform oil phase, stir at a speed of 150 rpm, and cool while stirring until it reaches 20 °C;

[0052] (2) Prepare an aqueous solution containing antioxidant active substances as the aqueous phase;

[0053] (3) Add the aqueous phase to the oil phase with stirring at a speed of 250 rpm, and the addition amount each time is 30 μL to obtain the antioxidant composition.

[0054] Preparation Example 3

[0055] This preparation example provides a preparation method of an antioxidant composition based on reverse micelle synergistic technology, including the following steps:

[0056] (1) Dissolve phospholipids, non-polar solvents and antioxidants at 90 °C to form a uniform oil phase, stir at a speed of 50 rpm, and cool while stirring until it reaches 30 °C;

[0057] (2) Prepare an aqueous solution containing antioxidant active substances as the aqueous phase;

[0058] (3) Add the aqueous phase to the oil phase with stirring at a speed of 350 rpm, and the addition amount each time is 10 μL to obtain the antioxidant composition.

[0059] Preparation Example 4

[0060] This preparation example provides a preparation method of an antioxidant composition based on reverse micelle synergistic technology, and the difference from Preparation Example 1 is only that the stirring speed in step (1) is 20 rpm.

[0061] Preparation Example 5

[0062] This preparation example provides a preparation method of an antioxidant composition based on reverse micelle synergistic technology, and the difference from Preparation Example 1 is only that the stirring speed in step (1) is 180 rpm.

[0063] Preparation Example 6

[0064] This Preparation Example provides a method for preparing an antioxidant composition based on reverse micelle synergistic technology, which is only different from Preparation Example 1 in that the stirring speed in step (3) is 220 rpm.

[0065] Preparation Example 7

[0066] This Preparation Example provides a method for preparing an antioxidant composition based on reverse micelle synergistic technology, which is only different from Preparation Example 1 in that the stirring speed in step (3) is 380 rpm.

[0067] Example 1

[0068] In this example, an antioxidant composition based on reverse micelle synergistic technology was prepared using the preparation method provided in Preparation Example 1. The raw materials for preparation were 35 parts by weight of phospholipid, 56 parts by weight of isopropyl palmitate, 1.5 parts by weight of tocopheryl acetate, and 2 parts by weight of an aqueous solution containing hydroxytyrosol. The mass percentage content of hydroxytyrosol in the aqueous solution containing hydroxytyrosol was 10%.

[0069] Example 2

[0070] In this example, an antioxidant composition based on reverse micelle synergistic technology was prepared using the preparation method provided in Preparation Example 2. The raw materials for preparation were 50 parts by weight of phospholipid, 45 parts by weight of isopropyl palmitate, 1 part by weight of tocopheryl acetate, and 3 parts by weight of an aqueous solution containing hydroxytyrosol. The mass percentage content of hydroxytyrosol in the aqueous solution containing hydroxytyrosol was 1%.

[0071] Example 3

[0072] In this example, an antioxidant composition based on reverse micelle synergistic technology was prepared using the preparation method provided in Preparation Example 3. The raw materials for preparation were 20 parts by weight of phospholipid, 78 parts by weight of isopropyl palmitate, 2 parts by weight of tocopheryl acetate, and 1 part by weight of an aqueous solution containing hydroxytyrosol. The mass percentage content of hydroxytyrosol in the aqueous solution containing hydroxytyrosol was 20%.

[0073] Examples 4 - 7

[0074] This example provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that it is prepared using the methods of Preparation Examples 4 - 7 respectively.

[0075] Example 8

[0076] This example provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that tocopheryl acetate is replaced by butylated hydroxyanisole.

[0077] Example 9

[0078] This embodiment provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that tocopheryl acetate is replaced by butylated hydroxytoluene.

[0079] Example 10

[0080] This embodiment provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that tocopheryl acetate is not added, and the proportion of tocopheryl acetate is distributed to phospholipids and isopropyl palmitate proportionally.

[0081] Example 11

[0082] This embodiment provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that hydroxytyrosol is replaced by ergothioneine.

[0083] Example 12

[0084] This embodiment provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that hydroxytyrosol is replaced by dipotassium glycyrrhizinate.

[0085] Comparative Example 1

[0086] This comparative example prepared a hydroxytyrosol solution with the same preparation raw materials as in Example 1, and the preparation method was to simply mix the preparation raw materials provided in Example 1.

[0087] Comparative Example 2

[0088] This comparative example prepared an ergothioneine solution with the same preparation raw materials as in Example 11, and the preparation method was to simply mix the preparation raw materials provided in Example 11.

[0089] Comparative Example 3

[0090] This comparative example prepared a dipotassium glycyrrhizinate solution with the same preparation raw materials as in Example 12, and the preparation method was to simply mix the preparation raw materials provided in Example 12.

[0091] Comparative Example 4

[0092] This comparative example provides an antioxidant composition based on reverse micelle synergistic technology, which is only different from Example 1 in that hydroxytyrosol is replaced by an equal amount of water.

[0093] Test Example 1

[0094] This test example observed the microstructure of the antioxidant composition based on the reverse micelle synergistic technology prepared in Example 1 using a transmission electron microscope, including the following steps: aspirate 10 μL of the sample and drop it onto the copper grid for precipitation for 1 min, then use filter paper to absorb the floating liquid; drop 10 μL of uranyl acetate staining solution onto the copper grid for precipitation for 1 min, and use filter paper to absorb the floating liquid; dry at room temperature for several minutes; perform electron microscopy detection and imaging at 80 - 120 kv, and collect and analyze the images.

[0095] The results of the transmission electron microscope are as Figure 1 shown, and the product prepared in Example 1 presents a reverse micelle structure of nanoscale spherical shape.

[0096] Test Example 2

[0097] This test example determined the DPPH antioxidant activity of the products prepared in the examples and comparative examples. DPPH, also known as 1,1-diphenyl-2-picrylhydrazyl, is a very stable nitrogen-centered free radical. Its stability mainly comes from the steric hindrance of the 3 benzene rings with resonance stabilization, which makes the unpaired electrons on the nitrogen atom in the middle unable to play their due electron pairing role. DPPH is purple in anhydrous ethanol or methanol solution, has a maximum absorption at a wavelength of 517 nm, and the absorbance has a linear relationship with the concentration. When a free radical scavenger is added to it, it can combine with or replace DPPH, reducing the number of free radicals, decreasing the absorbance, and making the solution color lighter. Thus, the ability to scavenge free radicals can be evaluated.

[0098] When determining the antioxidant activity, the sample addition amounts for each group are shown in Table 1. After shaking well, react at room temperature in the dark for 30 min, detect the absorbance at 517 nm with an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the DPPH scavenging rate. The results are shown in Table 2. The formula for calculating the DPPH scavenging rate is: Scavenging rate (%) = (1 - (A1 - A2) / A0) × 100.

[0099] Table 1

[0100]

[0101] Table 2

[0102]

[0103]

[0104] It can be seen from Table 2 that:

[0105] (1) By comparing Example 1 with Comparative Example 1, Example 11 with Comparative Example 2, and Example 12 with Comparative Example 3, it can be seen that after reverse micelle encapsulation, the antioxidant activity of the antioxidant active ingredients can be enhanced, and it can play a synergistic effect when applied to cosmetics;

[0106] (2) By comparing Example 1 with Examples 4 - 7, it can be seen that the stirring speed of the oil phase affects the dissolution rate of lecithin. The slower the stirring speed, the longer the dissolution time, and thus the higher the oxidation degree of lecithin. If the stirring speed is too fast, it is also easy for lecithin to contact more air and be oxidized; while the stirring speed of the water phase affects the stability of the reverse micelle structure formed. When the stirring speed is relatively low, the active substances cannot be completely wrapped by the reverse micelle structure, forming a free phase, and the subsequent reverse micelles will gradually become unstable over time;

[0107] (3) By comparing Example 1, Examples 8 - 12 and Comparative Example 4, it can be seen that tocopheryl acetate and hydroxytyrosol have a synergistic antioxidant effect. If either one is missing or replaced, the antioxidant effect will become worse.

[0108] Test Example 3

[0109] In this test example, hydroxytyrosol, which is prone to discoloration, is taken as an example for easy observation. The samples prepared in Example 1 and Comparative Example 1 are placed in an incubator at 45°C for 120 days, and it is observed whether discoloration occurs to judge the change in the activity of hydroxytyrosol. The results are as Figure 2 shown. The left figure is the hydroxytyrosol solution provided by Comparative Example 1, and the right figure is the antioxidant composition containing hydroxytyrosol based on the reverse micelle synergistic technology provided by Example 1. The initial color of hydroxytyrosol is colorless and transparent. After 120 days of constant temperature treatment at 45°C, Comparative Example 1 turns into reddish-brown, while Example 1 still maintains the light yellow color of the reverse micelle.

[0110] The stability test results show that after being encapsulated by reverse micelles, the stability of hydroxytyrosol can be improved, and the discoloration phenomenon in cosmetics can be delayed. For antioxidant active ingredients that are prone to oxidation and have poor stability, the reverse micelle technology can greatly improve their stability.

[0111] Test Example 4

[0112] In this test example, safety evaluation tests were carried out on the products provided by the examples and comparative examples. Women aged 18 - 35 years old without a history of allergies were selected, and each subject tried the samples. After cleaning the back of the subject, the patch tester with the sample added was applied to the selected position on the back with a non-irritating tape. After pasting, gently press it with fingers to make it evenly adhere to the skin and keep it for 48h. The subjects should keep the patch area dry within 48h, and avoid strenuous exercise, scratching the patch area, long-term sunlight exposure, etc. After 48h, remove the tester and make marks. After 30min, when the indentation disappears, judge it under sufficient light.

[0113] The test results show that all the subjects had negative reactions, no irritation to the skin, and no positive reactions, indicating that the products provided by the present invention are safe and mild.

[0114] In summary, in the antioxidant composition provided by the present invention, the antioxidant active substance is encapsulated by reverse micelles, the stability of the antioxidant active substance is significantly improved, and the antioxidant efficacy is increased by more than 17%. This method can be used for various water-soluble antioxidant active substances and has broad application prospects.

[0115] The applicant declares that the above description is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by any person skilled in the art within the technical scope disclosed by the present invention fall within the protection scope and the disclosure scope of the present invention.

Claims

1. An antioxidant composition based on reverse micelle synergistic technology, characterized in that, The antioxidant composition includes a reverse micelle structure and an aqueous solution containing an antioxidant active substance, and the reverse micelle structure encapsulates the aqueous solution containing the antioxidant active substance.

2. The antioxidant composition according to claim 1, wherein The raw materials for preparing the reverse micelle structure include phospholipids, non-polar solvents, and antioxidants; Preferably, the purity of the phospholipids is 90% - 99%; Preferably, the non-polar solvent includes any one or a combination of at least two of isopropyl palmitate, isopropyl myristate, or isopropyl laurate; Preferably, the antioxidant includes any one or a combination of at least two of butylated hydroxyanisole, butylated hydroxytoluene, tert-butyl hydroquinone, or tocopheryl acetate.

3. The antioxidant composition according to claim 1 or 2, wherein The raw materials for preparing the reverse micelle structure include 20 - 50 parts by weight of phospholipids, 45 - 78 parts by weight of non-polar solvents, and 1 - 2 parts by weight of antioxidants.

4. The antioxidant composition according to any one of claims 1 to 3, characterized in that The antioxidant active substances in the aqueous solution containing antioxidant active substances include any one or a combination of at least two of hydroxytyrosol, ergothioneine, or dipotassium glycyrrhizinate; Preferably, the mass percentage content of the antioxidant active substance in the aqueous solution containing the antioxidant active substance is 1% - 50%.

5. The antioxidant composition according to any one of claims 1 to 4, characterized in that The raw materials for preparing the antioxidant composition include phospholipids, non-polar solvents, antioxidants, and an aqueous solution containing antioxidant active substances; Preferably, the raw materials for preparing the antioxidant composition include 20 - 50 parts by weight of phospholipids, 45 - 78 parts by weight of non-polar solvents, 1 - 2 parts by weight of antioxidants, and 1 - 3 parts by weight of an aqueous solution containing antioxidant active substances.

6. A method for preparing an antioxidant composition according to any one of claims 1 to 5, characterized in that, The preparation method includes the following steps: (1) Dissolve the phospholipids, non-polar solvents, and antioxidants to form a uniform oil phase, and cool while stirring; (2) Prepare an aqueous solution containing antioxidant active substances as the water phase; (3) Add the water phase to the oil phase while stirring to obtain the antioxidant composition.

7. The preparation method according to claim 6, wherein The temperature of the dissolution in step (1) is 70 - 90°C; Preferably, the stirring speed in step (1) is 50 - 150 rpm; Preferably, the final temperature of the cooling in step (1) is 20 - 30°C.

8. The preparation method according to claim 6, characterized in that, The stirring speed in step (3) is 250 - 350 rpm; Preferably, the amount added each time in the added water phase in step (3) is 10 - 30 μL.

9. Use of the antioxidant composition according to any one of claims 1 - 5 in the preparation of cosmetics.

10. The application according to claim 9, wherein The cosmetics include any one or a combination of at least two of emulsions, lotions, eye creams, essence milks, creams, or facial masks.

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