Compound sarcandra glabra anti-inflammatory face cream and preparation method thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly discloses a compound sarcandra glabra anti-inflammatory face cream and a preparation method thereof, the compound sarcandra glabra anti-inflammatory face cream comprises the following components in percentage by weight: a phase A: 10% of liquid paraffin, 2% of stearic acid, 3-4% of octadecanol, 4-4.5% of glyceryl monostearate and 0.2% of ethylparaben; a phase B: 16-17% of glycerol, 4-4.5% of Tween 60 and the balance of refined water; and a phase C: 0.16%-0.2% of a ventilago leiocarpa benth extract, 0.08%-0.1% of a sarcandra glabra extract and 0.16%-0.2% of a Chinese mahonia stem extract. The sarcandra glabra extract, the ventilago leiocarpa benth extract and the Chinese mahonia stem extract are compounded to serve as effective active ingredients to prepare the face cream, and the prepared face cream has a very good anti-inflammatory effect, can effectively achieve the effects of inhibiting bacteria, resisting inflammation, relieving skin inflammation, improving skin red and swollen acne marks and the like on face skin, and has a good anti-inflammatory effect. The repair and regeneration of the skin can be promoted. And the prepared face cream is uniform in texture, good in stability, safe, mild, non-irritant and good in moisturizing effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and particularly relates to a compound Sarcandra glabra anti-inflammatory cream and a preparation method thereof. Background Art

[0002] Under the pressure of the modern living environment, the skin is constantly stimulated by the external environment such as physical stimuli, pathogenic microorganism stimuli, and life pressure. Once these stimuli exceed the speed and intensity of skin recovery, the skin barrier will be continuously damaged. When the skin barrier is damaged, the skin releases inflammatory factors, triggering inflammatory damage, and ultimately leading to skin problems, such as allergies, flushing, itching, pigmentation, and inflammatory reactions, which affect appearance. With the rapid economic development and social progress, people's pursuit of appearance is getting higher and higher, and the care of facial skin has attracted particular attention. Facial cream is an important step in basic skin care. Skin inflammations such as acne and acne have troubled many people. Therefore, it is particularly important to develop an anti-inflammatory cream product that can improve skin quality and relieve symptoms such as flushing, itching, and inflammatory reactions.

[0003] With the continuous improvement of people's living standards, people's requirements for beauty and skin care cosmetics are also getting higher and higher. They not only require functions such as moisturizing and anti-inflammatory, but also the requirements for cosmetic raw materials are increasingly developing in the direction of "naturalization". Most of the current beauty and skin care cosmetics on the market contain irritating chemicals and harmful substances to the human body. These skin care products are likely to cause people's skin to become thinner, skin allergies, and even damage to human organs and functions. Therefore, people pay more attention to the quality and safety of cosmetic raw materials and expect to use green natural raw materials that have skin care and beauty effects and are harmless to human health. The materials used in cosmetics have characteristics such as good stability and no allergic reaction to human skin.

[0004] How to prepare green, safe, and efficient skin care products has always been a hot topic in the cosmetics industry. Due to their high safety performance and good efficacy, Chinese herbal plant extracts are widely used in cosmetics. Sarcandra glabra is also called Saururus chinensis, Sambucus williamsii, etc., and belongs to Chloranthaceae Sarcandra Sarcandra glabraThe dried whole plant of Sarcandra glabra (Thunb.) Nakai contains volatile oils, esters, phenols, tannins, flavonoids, cyanogenic glycosides, coumarins, lactones and other components. The whole plant of Sarcandra glabra can be used medicinally, with the effects of clearing heat and detoxifying, expelling wind and promoting blood circulation, reducing swelling and alleviating pain, and antibacterial and anti-inflammatory. Pharmacological studies have shown that the extracts of Sarcandra glabra have various effects such as anti-inflammatory, antibacterial, anti-tumor and neuroprotective effects. Among them, the main anti-inflammatory components such as Sarcandra glabra polysaccharides, lindenane sesquiterpene polymers and coumarins have good anti-inflammatory activities. The extracts of Sarcandra glabra can reduce the release of inflammatory factors and inhibit the proliferation of inflammatory cells, thus alleviating various skin inflammations such as eczema, dermatitis and allergies. Sarcandra glabra has certain antibacterial and antiviral effects on some pathogenic microorganisms such as Staphylococcus aureus, Klebsiella pneumoniae, herpes simplex virus, etc. The flavonoid compounds of Sarcandra glabra have strong antioxidant effects, can scavenge harmful substances such as peroxides and free radicals, and can also enhance the body's immunity, preventing and treating tumors and chronic diseases. Therefore, extracting effective active ingredients from Sarcandra glabra for the preparation of skin care products has broad application prospects. Summary of the Invention

[0005] The purpose of the present invention is to provide a compound Sarcandra glabra anti-inflammatory facial cream and its preparation method. The facial cream is prepared by compounding extracts of Sarcandra glabra, Millettia dielsiana and Mahonia bealei as effective active ingredients. The prepared facial cream has obvious anti-inflammatory effects, can effectively inhibit bacteria and reduce inflammation on facial skin, relieve skin inflammation, improve redness and acne marks, etc., and can promote skin repair and regeneration; moreover, the prepared facial cream has uniform texture, good stability, is safe and mild, non-irritating, and has good moisturizing effect.

[0006] The technical solution adopted by the present invention is as follows: A compound Sarcandra glabra anti-inflammatory facial cream, comprising the following components in percentage by weight: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 3 - 4%, glycerol monostearate 4 - 4.5%, ethylparaben 0.2%; Phase B: glycerol 16 - 17%, Tween 60 4 - 4.5%, purified water the balance; Phase C: extract of Millettia dielsiana 0.16% - 0.2%, extract of Sarcandra glabra 0.08% - 0.1%, extract of Mahonia bealei 0.16% - 0.2%.

[0007] The extraction method of the extract of Sarcandra glabra is as follows: Take the crude powder of Sarcandra glabra, add water according to the ratio of material-liquid ratio of 1:10 (g / mL), reflux and extract twice, 2 hours each time, filter and combine the filtrates for rotary evaporation, and concentrate to 2 g / mL to obtain the extract of Sarcandra glabra.

[0008] Preferably, the above Compound Sarcandra glabra Anti-inflammatory Facial Cream comprises the following components in percentage by weight: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 4%, glyceryl monostearate 4%, ethylparaben 0.2%; Phase B: glycerol 16%, polysorbate 60 4%, purified water the balance; Phase C: extract of Millettia dielsiana 0.16%, extract of Sarcandra glabra 0.08%, extract of Mahonia bealei 0.16%.

[0009] Furthermore, the extraction method of the extract of Mahonia bealei is as follows: adopting the heat reflux extraction method, adding 8 times the weight of 70% ethanol to a certain amount of Mahonia bealei medicinal materials, refluxing and extracting 3 times, combining the filtrates and concentrating to 0.2 g / mL to obtain the extract of Mahonia bealei.

[0010] The extraction method of the extract of Millettia dielsiana is as follows: taking Millettia dielsiana, adding water according to the ratio of material-liquid ratio of 1:10 (g / mL), decocting and extracting for 1 time, 3 hours each time; filtering, adding water to the filter residue according to the ratio of material-liquid ratio of 1:8 (g / mL), decocting for 1 hour; filtering, combining the filtrates and concentrating to 2 g / mL to obtain the extract of Millettia dielsiana.

[0011] The preparation method of the above Compound Sarcandra glabra Anti-inflammatory Facial Cream comprises the following steps: (1) According to the percentage by weight, take each raw material component for standby, accurately weigh out Phase A and Phase B, and heat them to 80 °C respectively until completely melted; (2) Add Phase B to the reaction tank and stir and homogenize for 5 - 7 minutes, then slowly add the substances of Phase A to the substances of Phase B, make them fully contact and mix evenly, and carry out emulsification for 10 minutes; (3) Finally, add the substances of Phase C, mix evenly, stir and cool to 50 °C, stop stirring, cool to room temperature, and discharge and package.

[0012] In the present invention, the Sarcandra glabra used is the dried whole plant of Sarcandra glabra (Thunb.) Nakai of the family Chloranthaceae. Sarcandra glabra contains rich bioactive substances, such as flavonoids, saponins, and polysaccharide compounds, etc. These substances have significant anti-inflammatory effects, can inhibit skin inflammatory reactions, relieve discomfort such as swelling, redness, and itching, and alleviate various skin inflammations, such as eczema, dermatitis, and allergies. The polysaccharide compounds in Sarcandra glabra have good moisturizing effects, can effectively lock in moisture, prevent water loss, and maintain the skin's moisture content. Sarcandra glabra also has good repair functions and can accelerate the skin wound healing process. Sarcandra glabra is rich in various antioxidant substances, such as flavonoids and vitamin C, etc. These substances can neutralize free radicals, reduce the damage caused to the skin by environmental pollution and ultraviolet radiation, delay the skin aging process, and protect the skin from external damage. Sarcandra glabra (Thunb.) Nakai, and the extract of Sarcandra glabra contains rich bioactive substances, such as flavonoids, saponins, and polysaccharide compounds, etc. These substances have significant anti-inflammatory effects, can inhibit skin inflammatory reactions, relieve discomfort such as swelling, redness, and itching, and alleviate various skin inflammations, such as eczema, dermatitis, and allergies. The polysaccharide compounds in Sarcandra glabra have good moisturizing effects, can effectively lock in moisture, prevent water loss, and maintain the skin's moisture content. Sarcandra glabra also has good repair functions and can accelerate the skin wound healing process. Sarcandra glabra is rich in various antioxidant substances, such as flavonoids and vitamin C, etc. These substances can neutralize free radicals, reduce the damage caused to the skin by environmental pollution and ultraviolet radiation, delay the skin aging process, and protect the skin from external damage.

[0013] The Millettia dielsiana mentioned above is the plant Ventilago leiocarpa Benth. of the family Rhamnaceae Ventilago leiocarpaBenth. The root or stem of Caulis Sanguinea mainly contains alkaloids, flavonoid glycosides, amino acids and anthraquinones, which have the effects of dispelling wind and relieving itching, promoting blood circulation and removing blood stasis, promoting diuresis and reducing swelling, protecting the liver, and anti-inflammatory and analgesic. Caulis Sanguinea can be used to relieve swelling and pain caused by various inflammations, such as arthritis, muscle sprains and soft tissue injuries, and can inhibit the release of inflammatory mediators such as histamine, thereby achieving the effect of relieving itching. Decoction of the decoction for external washing or mashing and applying to the affected area has a certain effect on relieving symptoms such as skin itching and eczema.

[0014] The mahonia tree is a plant of the genus mahonia of the Berberidaceae family, mahonia latifolia Mahonia bealei (Fort.) Carr., Mahonia M.fortunei The stem or bark of (Lindl.) Fedde has the effects of clearing heat, drying dampness and detoxifying. Mahonia has strong antibacterial and anti-inflammatory effects, and has inhibitory and killing effects on a variety of bacteria and fungi. Its ethanol extract can be used to treat skin infections, inflammation and other problems, to relieve symptoms such as skin eczema and itching, and can also promote skin repair and regeneration. It has a certain therapeutic effect on skin damage, burns and scalds. Mahonia leaf extract can inhibit the release of inflammatory factors and relieve inflammatory symptoms. It can be used as a skin care product to relieve skin inflammation and improve acne and acne. In addition, Mahonia contains rich natural antioxidants such as polyphenols and flavonoids, which can neutralize free radicals, protect cells from oxidative damage, delay skin aging, and improve skin elasticity and luster.

[0015] The beneficial effects of the present invention are: 1. The present invention extracts effective active ingredients from three natural plants, namely, Caulis Sanguisorba Officinalis, Coral Grass and Mahoniae. The extracted Caulis Sanguisorba Officinalis, Coral Grass and Mahoniae contain active ingredients such as flavonoids, saponins and polysaccharide compounds, have strong antibacterial and anti-inflammatory effects, can be used to treat skin inflammation problems, relieve symptoms such as inflammation, itching, eczema, etc., and the natural antioxidant substances such as flavonoids contained can protect cells from oxidative damage and protect the skin from external damage. The facial cream prepared by compounding these three extracts has good anti-inflammatory effects, can effectively inhibit bacteria and inflammation on the facial skin, relieve skin inflammation, relieve redness and itching, and improve skin quality, can improve skin redness, swelling and acne marks, and promote skin repair and regeneration, and is green and safe.

[0016] 2. Through single-factor experiments and orthogonal experiments for optimization, the optimal formula and preparation process for the compound Sarcandra glabra anti-inflammatory facial cream of the present invention are obtained. The facial cream prepared by the method of the present invention has a uniform texture, is delicate, has a moderate viscosity, is easy to spread, has good stability and is not prone to layering, has no granular feeling, is a light yellow paste-like solid, has no bad smell, and its pH value and stability meet the standard requirements. Moreover, the rat skin irritation experiment shows that the compound Sarcandra glabra anti-inflammatory facial cream prepared by the method of the present invention has no irritation.

[0017] 3. The present invention provides a compound Sarcandra glabra anti-inflammatory facial cream and a preparation method thereof. The compound Sarcandra glabra anti-inflammatory facial cream prepared by the method of the present invention is safe, mild, non-irritating, has a good moisturizing effect, has a good anti-inflammatory effect, can effectively relieve and improve skin inflammation, promote the repair and regeneration of the skin, and make the skin recover to health. Description of the Drawings

[0018] Figure 1 Sample diagram of the compound Sarcandra glabra anti-inflammatory facial cream prepared by the method of the present invention; Figure 2 Radar chart of the comprehensive evaluation of the compound Sarcandra glabra anti-inflammatory facial cream by testers; Figure 3 Changes in the skin of rats after a single administration of the compound Sarcandra glabra anti-inflammatory facial cream; Figure 4 Changes in the skin of rats after multiple administrations of the compound Sarcandra glabra anti-inflammatory facial cream. Detailed Embodiments

[0019] Example 1 Research on the compound Sarcandra glabra anti-inflammatory facial cream 1 Experimental method 1.1 Extraction of medicinal materials (1) Sarcandra glabra extract: Take 100 g of Sarcandra glabra crude powder, add water according to the solid-liquid ratio of 1:10 (g / mL), reflux and extract twice, each time for 2 hours. After filtration, combine the filtrates and perform rotary evaporation, concentrating to 2 g / mL to obtain the Sarcandra glabra extract.

[0020] (2) Mahonia bealei extract: Adopt the hot reflux extraction method, add a certain amount of Mahonia bealei to 8 times the amount of 70% ethanol as the extraction agent, reflux and extract 3 times, and combine the filtrates and concentrate to 0.2 g / mL.

[0021] (3) Millettia dielsiana extract: Take 100 g of Millettia dielsiana, add water according to the solid-liquid ratio of 1:10, decoct and extract once, each time for 3 h; filter, add water to the filter residue according to the solid-liquid ratio of 1:8 (g / mL), decoct for 1 h; filter, and combine the filtrates and concentrate to 2 g / mL.

[0022] 1.2 Preparation of the compound Sarcandra glabra anti-inflammatory facial cream Accurately weigh out Phase A and Phase B, and heat them to 85°C respectively until completely melted. Add Phase B to the reaction tank and stir and homogenize for about 5 minutes. Then slowly add the substances in Group A to Group B to make them fully contact and mix evenly for emulsification. After the addition of the raw materials in Group A is complete, add Group C and mix evenly. Stir and cool to 50°C, stop stirring, and cool to room temperature for packaging.

[0023]

[0024] 1.3 Single-factor experiment 1.3.1 Screening of the proportion of oil phase components Weigh 5 portions of liquid paraffin (X), stearic acid (Y), and octadecanol (Z). The mass fraction of liquid paraffin is fixed at 10%. The above three are in different ratios (5:1:1; 5:1:2; 5:2:2; 5:3:3; 5:1:3). The dosage of glyceryl monostearate in each portion is 3%, the dosage of glycerol is 18%, the dosage of Tween is 3%, the dosage of Mahonia fortunei extract is 0.08%, the dosage of Sarcandra glabra extract is 0.08%, the dosage of Millettia dielsiana extract is 0.08% (the minimum dosage within the range), and the dosage of ethylparaben is 0.2%. Examine the samples prepared with different ratios of liquid paraffin, stearic acid, and octadecanol. Score with the overall scores of the appearance properties, viscosity, spreadability, and cold and heat stability of the samples as the evaluation indexes to screen the dosage of the oil phase base material.

[0025] 1.3.2 Screening of the emulsifier content Weigh 5 portions of glyceryl monostearate and Tween 60 (1:1) with contents of 6%, 7%, 8%, 9%, and 10% respectively. In each portion, add 18% of glycerol according to the pre-prescription, the dosage of Mahonia fortunei extract is 0.08%, the dosage of Sarcandra glabra extract is 0.08%, the dosage of Millettia dielsiana extract is 0.08% (the minimum dosage within the range), the dosage of ethylparaben is 0.2%, and prepare samples with liquid paraffin, stearic acid, and octadecanol according to the results screened in 1.3.1. Score with the overall scores of the appearance properties, viscosity, spreadability, and cold and heat stability of the samples as the evaluation indexes to screen the dosage of the emulsifier.

[0026] 1.3.3 Screening of the dosage of the humectant Weigh 5 portions of glycerol with contents of 12%, 14%, 16%, 18%, and 20% respectively. In each portion, add the dosage of Mahonia fortunei extract of 0.08%, the dosage of Sarcandra glabra extract of 0.08%, the dosage of Millettia dielsiana extract of 0.08% (the minimum dosage within the range), 0.2% of ethylparaben, and prepare samples with liquid paraffin, stearic acid, and octadecanol according to the results screened in 1.3.1 and glyceryl monostearate and Tween 60 according to the results screened in 1.3.2. Examine the dosage of glycerol, score with the overall scores of the appearance properties, viscosity, spreadability, and cold and heat stability of the samples and the moisture retention rate as the evaluation indexes, and measure the moisture retention rate to determine the optimal content of the humectant.

[0027] Method for measuring the moisture retention rate: Weigh a certain amount of the sample, place it in a petri dish of appropriate size, and leave it at about 25 °C at room temperature in an environment with a relative humidity of 43% and 81% for 24 h. Weigh the mass change and calculate the moisture retention rate. Calculate the moisture retention rate: (m - m0) / m0 × 100%. m0 and m represent the mass of the sample before and after placement, unit: g. The smaller the moisture retention rate, the better the moisture retention.

[0028] 1.3.4 Screening of the dosage of the main drug Weigh 5 portions of each of the compound extracts, and the dosages of the extracts of Millettia dielsiana, Sarcandra glabra, and Mahonia bealei are 1:1:1; 2:1:1; 2:1:2; 3:2:2; 3:1:2 respectively. Add 0.2% of ethylparaben according to the preliminary prescription, and prepare the samples with liquid paraffin, stearic acid, and stearyl alcohol according to the results screened in 1.3.1, with glyceryl monostearate and Tween 60 according to the results screened in 1.3.2, and with glycerol according to the results screened in 1.3.3. Take the color, smell, and fineness of the samples as the evaluation indexes to determine the optimal dosage of the main drug.

[0029] 1.3.5 Investigation of the emulsification time Accurately weigh the phase A, phase B, and phase C in the amounts screened above, and emulsify them for 10 min, 15 min, and 20 min respectively under the same conditions with an emulsification temperature of 85 °C. Take the overall evaluation scores of the appearance properties, viscosity, spreading and dispersibility, and cold and heat resistance stability of the samples as the evaluation indexes to select the best emulsification time.

[0030] 1.3.6 Investigation of the emulsification temperature Accurately weigh the phase A, phase B, and phase C, heat and dissolve them, and divide them into 3 equal parts on average. Under the condition of an emulsification time of 10 min, set the emulsification temperatures to 80 °C, 85 °C, and 90 °C respectively. Take the overall evaluation scores of the appearance properties, viscosity, spreading and dispersibility, and cold and heat resistance stability of the samples as the evaluation indexes to select the best emulsification temperature.

[0031] 1.4 Optimization by orthogonal experiment Analyze four factors, namely the oil phase (A), emulsifier (B), moisturizer (C), and emulsification temperature (D), as the investigation objects, and each factor has three levels. According to the L 9 (3 4 ) orthogonal experiment table to design the experiment. Take the overall evaluation scores of the appearance properties, viscosity, spreading and dispersibility, and cold and heat resistance stability of the samples as the evaluation indexes to select the best formulation ratio of the Sarcandra glabra cream, as shown in Tables 2 and 3 specifically.

[0032]

[0033]

[0034] 1.5 Stability Evaluation of Facial Cream 1.5.1 Sensory Evaluation Observe under indoor conditions and out of direct sunlight. Apply the sample on the skin and observe visually to check if the appearance is delicate, the color and luster are bright, and there are no visible individual particles or bubbles to the naked eye.

[0035] 1.5.2 pH Value Determination Weigh 2 g of each of the 3 batches of samples to be tested, add 10 ml of distilled water, dilute and stir evenly. After standing for a period of time, obtain a homogeneous emulsion and measure the pH with a pH meter. Repeat 3 times in parallel and calculate the average value.

[0036] 1.5.3 Cold Resistance Test Put 5 g of the sample in a beaker, seal it, and place it in a refrigerator at -10°C. Take it out after 24 h, and observe visually whether there is stratification or color change after returning to room temperature.

[0037] 1.5.4 Heat Resistance Test Put 5 g of the sample in a beaker, seal it, and place it in a constant temperature incubator at 40°C. Take it out after 24 h, and observe visually whether there is stratification or color change after returning to room temperature.

[0038] 1.5.5 Centrifugation Test Fill the centrifuge tubes with about 2 / 3 of the height of the 3 batches of samples, weigh them, and place them in the centrifuge to keep it balanced. Centrifuge at a speed of 2500 r / min for 10 min, and observe whether there is oil-water stratification.

[0039] 1.6 Questionnaire for Human Trial An experience survey of compound Sarcandra glabra anti-inflammatory facial cream was conducted on 50 recruited college students. Apply an appropriate amount of the sample evenly on the back of one hand, and leave the back of the other hand untreated as a control. After 15 min, wash it off with warm water, and then apply it once in the morning and once in the evening every day for 1 week for testing. Volunteers conducted a blind evaluation of the questionnaire on 6 major indicators of the facial cream product, including texture, mildness (whether there is a sense of irritation), moisturizing property, spreadability, absorbability, and the improvement effect on the skin, in accordance with QB / T 2872—2007 "Light Industry Standard of the People's Republic of China" and combined with their own subjective trial experience (Tables 4 and 5). The scoring is represented by a 5-point system, from 5 points to 1 point, which are judged as very good, good, general, poor, and very poor in turn.

[0040]

[0041]

[0042] 1.7 Skin Irritation Test on Rats The test was carried out with reference to the single-dose skin irritation and multiple-dose skin irritation methods in the "Technical Specifications for Cosmetics Safety".

[0043] 1.7.1 Single-dose irritation test Eight SD rats (half male and half female) were selected for adaptive feeding for 5 days. Their backs were depilated for 16 cm 24 h before the test 2 (4 cm × 4 cm). 1 g of compound Sarcandra glabra anti-inflammatory cream was evenly applied to the left depilated area of the rats' backs. The right skin area was used as the control area and fixed with non-irritating adhesive tape. After 24 h of occlusion, the residual test substance was removed with water. The skin reactions at the application site were observed at 1 h, 24 h, 48 h, and 72 h after removing the residual test substance, and the skin was scored for erythema, crusting, and edema formation according to the skin irritation response scoring criteria (Table 6). The comprehensive evaluation was based on the average integral value of the test animals

[0044] 1.7.2 Multiple-dose irritation test Eight SD rats (half male and half female, with females being non-pregnant and non-lactating) were selected for adaptive feeding for 5 days. 24 h before the test, 16 cm 24 h of hair was removed from both sides of the spinal column on the backs of the test animals 2 (4 cm × 4 cm). The left skin area was the drug administration area, and the right skin area was the control area. 1 g of compound Sarcandra glabra anti-inflammatory cream was evenly applied to the left skin area once a day. Hair removal was required before each application. The application was continued for 14 consecutive days. The skin reactions at the application site were observed at 1 h, 24 h, 48 h, and 72 h after removing the residual test substance. The drug administration area and the control area were scored according to Table 6, and the skin irritation intensity was judged based on the average integral value

[0045] 1.7.3 Skin irritation evaluation For single-dose and multiple-dose skin irritation tests, calculate the average score of the skin reaction integral of the test substances and excipients in each group at each observation time point according to Table 6. The average integral value at each observation time point = (erythema score + edema score) / 8; the average integral value = the sum of the integral means at the observation points after the end of drug administration / 8

[0046]

[0047]

[0048] 2. Results and analysis 2.1 Results of single-factor tests 2.1.1 Results of the content ratio of the base material in phase A As shown in Table 8, different ratios in phase A would result in different outcomes. Adding too much would cause greasiness, and some of the obtained samples would show granularity, layering, and difficulty in spreading. When the composition ratio of phase A was 5:1:2, that is, the content of liquid paraffin was 10%, the content of stearic acid was 2%, and the content of cetyl alcohol was 4%, the obtained cream was light yellow, with a delicate texture, easy to spread, and good stability

[0049]

[0050] 2.1.2 Influence Results of Emulsifier Content As can be seen from Table 9, the screening results of the emulsifier content show that as the amount of emulsifier increases, the viscosity of the sample increases, and the phenomenon of difficult coating appears. When the content of the emulsifier is 8%, that is, the content of glycerol monostearate is 4% and the content of Tween 60 is 4%, the quality of the cream is the best, and the obtained facial cream has a uniform, delicate texture, is easy to coat, and has good viscosity. This also indicates that the stability of the cream obtained by mixing multiple emulsifiers is higher than that of using a single emulsifier.

[0051]

[0052] 2.1.3 Influence of Different Dosages of Humectant As can be seen from Table 10, as the content of the humectant increases, the moisturizing effect also increases. However, when the dosage exceeds a certain limit, it will also cause the sample to show a layering phenomenon. In combination with the actual situation, considering that there are limitations in the use of glycerol, the dosage of glycerol is selected as 16%, and the obtained facial cream has the best effect.

[0053]

[0054] 2.1.4 Influence of Different Dosages of Main Drug As shown in Table 11, as the content of the compound main drug extract increases, the color will become darker and darker, which also affects the appearance and fineness of the cream. Considering the actual situation, it is finally decided that the content of the three main drug extracts added is 2:1:2, and the effect is the most ideal in all aspects.

[0055]

[0056] 2.1.5 Influence of Different Emulsification Times As can be seen from Table 12, the longer the emulsification time, the greater the influence on the facial cream. When the emulsification time is 10 min, the obtained cream has a uniform, delicate texture and is easy to coat.

[0057]

[0058] 2.1.6 Influence of Different Emulsification Temperatures As can be seen from Table 13, when the temperature increases, the effect of the cream is not good. When the emulsification temperature is 80 °C, the effect of the cream is the best, and it can also retain the active ingredients of the drug, shorten the cooling time, and the obtained cream has a delicate texture, is easy to apply, and is not easy to layer.

[0059]

[0060] 2.2 Results and Analysis of Orthogonal Experiments From the range in Table 14, it can be seen that the factors affecting the usability of the cream, in order of importance, are A > D > B > C. The optimal combination of process levels for the four factors is A2B3C3D1, that is, for the oil phase base material: the content of liquid paraffin is 10%, the content of stearic acid is 2%, and the content of cetyl alcohol is 4%; for the emulsifier: the content of glycerol monostearate is 4%, and the content of Tween 60 is 4%; for the moisturizer: the content of glycerol is 16%, and the emulsification temperature is 80 °C.

[0061]

[0062] 2.3 Evaluation Results of Cream Stability 2.3.1 Sensory Evaluation The texture of the cream is uniform, delicate, with moderate viscosity, easy to spread, no particle feeling, a light yellow paste solid, and no irritating bad smell, meeting the standard requirements.

[0063] 2.3.2 pH Measurement As shown in Table 15, the pH values of three batches of samples were measured, and the average value was 5.66. The specified range of the pH value of the cream is between 4.0 and 8.5, so it meets the standard requirements.

[0064]

[0065] 2.3.3 Physical and Chemical Properties As can be seen from Table 16 of the physical and chemical test results, the effects of the obtained cream meet the standard requirements.

[0066]

[0067] 2.4 Final Formula and Preparation Process of the Cream The final formula of the cream is shown in Table 17. The best preparation process is as follows: ① Accurately weigh out Phase A and Phase B, and heat them to 80 °C respectively until completely melted. ② Add Phase B to the reaction tank and stir and homogenize for about 5 minutes, then slowly add the substances in Group A to Group B to make them fully contact and mix evenly, and carry out emulsification for 10 minutes. ③ Finally, add the substances in Group C, mix evenly, stir and cool to 50 °C, stop stirring, and cool to room temperature for packaging. The sample diagram of the compound Sarcandra glabra anti-inflammatory cream prepared by the method of the present invention is shown in Figure 1 。

[0068]

[0069] 2.5 Analysis of Questionnaire Results 67 testers were aged between 15 and 25 years old, among which 30% had oily skin, 19% had dry skin, 18% had combination skin, and 3% had sensitive skin, and 94% of the testers were in the skin inflammation period. They made an objective evaluation of the compound Sarcandra glabra anti-inflammatory cream, and the results are as Figure 2, as shown in Table 18. Most users can accept the smell and color of this product. 58.2% of the testers think its texture is good or very good, 71.6% of the testers think it is mild and non-irritating, 92.5% of the testers think it has moisturizing property, the absorbency is moderate, the skin is relatively moist after use, 74.6% of the testers think it is easy to apply, and 82.1% of the testers think it has a certain improvement effect on skin redness and acne marks. After using the product, 91.0% of the testers have never had skin allergies or discomfort phenomena.

[0070]

[0071] 2.6 Skin irritation results 2.6.1 Observation results of rats at different time periods after drug removal in the single irritation experiment are as Figure 3 shown. After single administration, in individual rats, local skin erythema was formed due to licking and scratching, but no edema occurred.

[0072] It can be seen from Tables 19 and 20 that both the blank control group and the drug administration group have no irritation, and both values are in the range of 0 to 0.49 for the non-irritation judgment standard. As the treatment time prolongs, the average integral shows an increasing trend, but the skin irritation intensity shows no irritation during the observation period.

[0073]

[0074]

[0075] 2.6.2 In the multiple irritation experiment, from Figure 4 it can be seen that after multiple administrations, very few parts of the rats' skin formed local erythema, and no edema occurred.

[0076] It can be seen from Table 21 that all values of the control group and the drug administration group are in the range of 0 to 0.49 for the non-irritation judgment standard, indicating that the compound Sarcandra glabra anti-inflammatory cream has no irritation and has no obvious difference from the control group.

[0077]

[0078] Example 2 A compound Sarcandra glabra anti-inflammatory cream, comprising the following components in the following weight percentage contents: A phase: liquid paraffin 10%, stearic acid 2%, stearyl alcohol 4%, glycerol monostearate 4%, ethylparaben 0.2%; B phase: glycerol 16%, Tween 60 4%, purified water 59.4%; C phase: extract of Millettia dielsiana 0.16%, extract of Sarcandra glabra 0.08%, extract of Mahonia bealei 0.16%.

[0079] The extraction method of the Sarcandra glabra extract is as follows: Take the crude powder of Sarcandra glabra, add water according to the ratio of solid-liquid ratio 1:10 (g / mL), reflux extract twice, 2 hours each time, filter and combine the filtrates, then perform rotary evaporation and concentrate to 2 g / mL to obtain the Sarcandra glabra extract.

[0080] The extraction method of the Mahonia bealei extract is as follows: Adopt the hot reflux extraction method, add 8 times the weight of 70% ethanol to a certain amount of Mahonia bealei medicinal materials, reflux extract 3 times, combine the filtrates and concentrate to 0.2 g / mL to obtain it.

[0081] The extraction method of the Millettia dielsiana extract is as follows: Take Millettia dielsiana, add water according to the ratio of solid-liquid ratio 1:10 (g / mL), decoct and extract once, 3 hours each time; filter, add water to the filter residue according to the ratio of solid-liquid ratio 1:8 (g / mL), decoct for 1 hour; filter, combine the filtrates and concentrate to 2 g / mL to obtain it.

[0082] The preparation method of the compound Sarcandra glabra anti-inflammatory cream includes the following steps: (1) According to the weight percentage content, take each raw material component for standby, accurately weigh out phase A and phase B, and heat them to 80 °C respectively until completely melted; (2) Add phase B to the reaction tank and stir and homogenize for 5 minutes, then slowly add the substances in phase A to the substances in phase B, make them fully contact and mix evenly, and perform emulsification for 10 minutes; (3) Finally, add the substances in phase C, mix evenly, stir and cool to 50 °C, stop stirring, cool to room temperature, discharge and package. Example 3

[0083] A compound Sarcandra glabra anti-inflammatory cream, including the following components in the following weight percentage content: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 3%, glycerol monostearate 4%, ethylparaben 0.2%; Phase B: glycerol 16.5%, Tween 60 4.5%, refined water 59.35%; Phase C: Millettia dielsiana extract 0.18%, Sarcandra glabra extract 0.09%, Mahonia bealei extract 0.18%. The extraction methods of the Sarcandra glabra extract, the Mahonia bealei extract and the Millettia dielsiana extract are the same as those in Example 2.

[0084] The preparation method of the compound Sarcandra glabra anti-inflammatory cream includes the following steps: (1) According to the weight percentage content, take each raw material component for standby, accurately weigh out phase A and phase B, and heat them to 80 °C respectively until completely melted; (2) Add phase B to the reaction tank and stir and homogenize for 6 minutes, then slowly add the substances in phase A to the substances in phase B, make them fully contact and mix evenly, and perform emulsification for 10 minutes; (3) Finally, add the C-phase substance, mix evenly, stir and cool to 50 °C, stop stirring, cool to room temperature, discharge and package. Example 4

[0085] A compound Sarcandra glabra anti-inflammatory cream, comprising the following components in weight percentage: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 4%, glyceryl monostearate 4.5%, ethylparaben 0.2%; Phase B: glycerol 17%, Tween 60 4.5%, purified water 57.3%; Phase C: extract of Millettia dielsiana 0.2%, extract of Sarcandra glabra 0.1%, extract of Mahonia bealei 0.2%. The extraction methods of the extract of Sarcandra glabra, the extract of Mahonia bealei and the extract of Millettia dielsiana are the same as those in Example 2.

[0086] The preparation method of the compound Sarcandra glabra anti-inflammatory cream comprises the following steps: (1) According to the weight percentage content, take each raw material component for standby, accurately weigh out Phase A and Phase B, and heat them to 80 °C respectively until completely melted; (2) Add Phase B to the reaction tank and stir and homogenize for 7 min, then slowly add the Phase A substance to the Phase B substance, make them fully contact and mix evenly, and carry out emulsification for 10 min; (3) Finally, add the C-phase substance, mix evenly, stir and cool to 50 °C, stop stirring, cool to room temperature, discharge and package.

Claims

1. A compound Sarcandra glabra anti-inflammatory facial cream, characterized in that, Comprising the following components in weight percentage: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 3 - 4%, glycerol monostearate 4 - 4.5%, ethylparaben 0.2%; Phase B: glycerol 16 - 17%, Tween 60 4 - 4.5%, purified water the balance; Phase C: extract of Millettia dielsiana 0.16% - 0.2%, extract of Sarcandra glabra 0.08% - 0.1%, extract of Mahonia bealei 0.16% - 0.2%; The extraction method of the extract of Sarcandra glabra is as follows: taking the crude powder of Sarcandra glabra, adding water according to the ratio of material - liquid ratio of 1:10, g / mL, refluxing and extracting twice, each time for 2 hours, filtering and then combining the filtrates for rotary evaporation, concentrating to 2 g / mL to obtain the extract of Sarcandra glabra.

2. The compound Sarcandra glabra anti-inflammatory facial cream according to claim 1, wherein Comprising the following components in weight percentage: Phase A: liquid paraffin 10%, stearic acid 2%, cetyl alcohol 4%, glycerol monostearate 4%, ethylparaben 0.2%; Phase B: glycerol 16%, Tween 60 4%, purified water the balance; Phase C: extract of Millettia dielsiana 0.16%, extract of Sarcandra glabra 0.08%, extract of Mahonia bealei 0.16%.

3. The compound Sarcandra glabra anti-inflammatory facial cream according to claim 1 or 2, characterized in that, The extraction method of the extract of Mahonia bealei is as follows: adopting the hot reflux extraction method, adding 8 times the weight of 70% ethanol to a certain amount of Mahonia bealei medicinal materials, refluxing and extracting 3 times, combining the filtrates and concentrating to 0.2 g / mL to obtain it.

4. The compound Sarcandra glabra anti-inflammatory facial cream according to claim 1 or 2, characterized in that, The extraction method of the extract of Millettia dielsiana is as follows: taking Millettia dielsiana, adding water according to the ratio of material - liquid ratio of 1:10, g / mL, decocting and extracting once, each time for 3 h; filtering, adding water to the filter residue according to the ratio of material - liquid ratio of 1:8 g / mL, decocting for 1 h; filtering, combining the filtrates and concentrating to 2 g / mL to obtain it.

5. The preparation method of the compound Sarcandra glabra anti-inflammatory facial cream according to claim 1 or 2, characterized in that, Comprising the following steps: (1) According to the weight percentage, take each raw material component for standby, accurately weigh out Phase A and Phase B, and heat them to 80°C respectively until completely melted; (2) Add Phase B to the reaction tank and stir and homogenize for 5 - 7 min, then slowly add the Phase A substance to the Phase B substance, make them fully contact and mix evenly, and carry out emulsification for 10 min; (3) Finally, add the Phase C substance, mix evenly, stir and cool to 50°C, stop stirring, cool to room temperature, and discharge and package.

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