External silamectin drop for pets as well as preparation method and application of external silamectin drop

Abstract

The invention belongs to the technical field of insect killing for pets, and particularly discloses external-use selamectin drops for pets as well as a preparation method and application thereof.Rosemary volatile oil, selamectin and a gardenia fruit extract are combined, so that the use amount of selamectin can be effectively reduced, and the use amount of selamectin is reduced; meanwhile, the use of a penetration enhancer (the penetration enhancer, such as dipropylene glycol monomethyl ether, needs to be added in a traditional formula) is avoided, the rosemary volatile oil can be used for dissolving a lipid layer of an insect exoskeleton or a microbial cell membrane and destroying the integrity of a membrane structure so as to promote the penetration of other components, and the gardenia fruit extract can be combined with microbial cell membrane protein so as to promote the growth of the insect exoskeleton or the microbial cell membrane. And meanwhile, the gardenia fruit extract, the inhibition of synthesis of bacterial protein and nucleic acid and the selamectin kill parasites by interfering conduction of neural signals of polypide bodies, and the action mechanisms of the gardenia fruit extract, the inhibition of synthesis of bacterial protein and nucleic acid and the selamectin are mutually synergistic, so that the insecticidal and antibacterial effects are jointly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of pet insecticide, and in particular to a selamectin drop for external use on pets and a preparation method and application thereof. Background Art

[0002] As attention to pet health issues gradually increases, external parasites of pets (such as scabies, Demodex, ear mites, turtle mites, etc.) and skin diseases (such as bacterial dermatitis, fungal skin diseases, etc.) have become common problems.

[0003] Parasitic diseases have always been an important factor affecting the health of dogs and cats. On the one hand, parasites can directly infect dogs and cats, causing skin inflammation, weight loss, and damaged fur quality; on the other hand, many spiders and insects can act as carriers of some infectious and parasitic diseases, leading to the occurrence of insect-borne diseases. Therefore, controlling and eliminating parasites inside and outside dogs and cats is of great significance to protecting the health of humans and dogs and cats. However, most of the existing topical drugs have the disadvantages of insignificant therapeutic effects, long treatment cycles, unsuitability for long-term use, or easy to cause pets to develop drug resistance. Summary of the invention

[0004] The present invention provides a selamectin drop for external use on pets, a preparation method thereof and applications thereof. The drop of the present invention has excellent mite removal and antibacterial effects and can effectively treat parasitic diseases.

[0005] The present invention solves the technical problem by adopting the following technical solutions: A selamectin drop for external use on pets comprises the following components in percentage by weight: 0.05-0.4% antioxidant, 0.5-1.2% dipotassium glycyrrhizinate, 1.5-4% rosemary volatile oil, 3-6% selamectin, 3-8.5% gardenia fruit extract, 5-20% 1,2-propylene glycol, and the balance solvent.

[0006] Rosemary essential oil: Rosemary essential oil can dissolve the lipid layer of insect exoskeleton or microbial cell membrane and destroy the integrity of membrane structure. It inhibits the activity of acetylcholinesterase (AChE), interferes with nerve signal transmission and causes paralysis of insects. At the same time, rosemary essential oil has excellent antioxidant and anti-inflammatory effects and can repair skin inflammation caused by parasites or bacterial infections.

[0007] Selamectin has a broad-spectrum antibacterial effect. At the same time, selamectin can enhance the activity of glutamate-gated chloride channels (GluCl) in invertebrate neurons, leading to hyperpolarization of the insect's neuromuscular system, causing paralysis and death. It has excellent killing effects on a variety of parasites such as fleas, ticks, mites and nematodes.

[0008] Gardenia fruit extract: It kills bacteria and parasites by destroying the bacterial cell membrane and inhibiting the synthesis of bacterial proteins and nucleic acids. The gardenia fruit extract can bind to the microbial cell membrane protein, change the membrane permeability, promote the penetration of other components, and improve the utilization rate of other components.

[0009] The present invention creatively combines rosemary volatile oil, selamectin, and gardenia fruit extract, which can effectively reduce the usage amount of selamectin and avoid the use of penetration enhancers (traditional formulations need to add penetration enhancers such as dipropylene glycol methyl ether and other components). Rosemary volatile oil can dissolve the lipid layer of the insect exoskeleton or microbial cell membrane, destroy the integrity of the membrane structure, thereby promoting the penetration of other components. The gardenia fruit extract can bind to the microbial cell membrane protein, change the membrane permeability, further improve the penetration of other components, and improve the utilization rate. At the same time, the gardenia fruit extract inhibits the synthesis of bacterial proteins and nucleic acids, and selamectin kills parasites by interfering with the conduction of nerve signals in the worm body. The action mechanisms of the three are synergistic with each other, enhancing each other, reducing the drug resistance that may occur when using a single component, and jointly improving the insecticidal and antibacterial effects.

[0010] As a preferred embodiment of the present invention, the antioxidant includes at least one of butylated hydroxyanisole and 2,6-di-tert-butyl-p-cresol.

[0011] As a preferred embodiment of the present invention, the solvent includes at least one of dimethyl sulfoxide, α-pyrrolidone, dimethylformamide, and ethanol.

[0012] As a preferred embodiment of the present invention, the preparation method of the rosemary volatile oil is: adding rosemary powder to water, performing steam distillation to obtain an oil-water mixture, separating the aqueous phase, and obtaining rosemary volatile oil.

[0013] As a preferred embodiment of the present invention, the particle size of the rosemary powder is 50-400 mesh; The mass ratio of the rosemary powder to water is 1:(4-10); The distillation time is 4-10 h.

[0014] As a preferred embodiment of the present invention, the preparation method of the gardenia fruit extract is: Adding gardenia fruit powder, cellulase, β-glucanase, and xylanase to water, and performing enzymatic hydrolysis to obtain an enzymatic hydrolysate; Ultrafiltering the enzymatic hydrolysate first with an ultrafiltration membrane with a cut-off molecular weight of 500 Da, then with an ultrafiltration membrane with a cut-off molecular weight of 5000 Da, and drying to obtain the gardenia fruit extract.

[0015] As a preferred embodiment of the present invention, the mass ratio of gardenia fruit powder, cellulase, β-glucanase, xylanase, and water is 1:(0.01~0.02):(0.002~0.008):(0.001~0.005):(4~10).

[0016] As a preferred embodiment of the present invention, the particle size of the gardenia fruit powder is 50~400 mesh; The temperature of the enzymatic hydrolysis is 45~52 °C, and the time of the enzymatic hydrolysis is 2~6 h.

[0017] The present invention also provides a preparation method of a topical selamectin drop for pets, comprising the following steps: Mix rosemary volatile oil, 1,2-propanediol and a solvent evenly, and then add an antioxidant, dipotassium glycyrrhizinate, selamectin, and selamectin, and stir evenly to obtain a topical selamectin drop for pets.

[0018] The present invention also provides an application of a topical selamectin drop for pets in the preparation of an external disinfection drug for pets, characterized in that the external disinfection drug includes a pet anti-parasitic drug, a pet trematodiasis drug or a pet cestodiasis drug.

[0019] The beneficial effects of the present invention: The present invention combines rosemary volatile oil, selamectin, and gardenia fruit extract, which can effectively reduce the usage amount of selamectin, and at the same time avoid the use of penetration enhancers (traditional formulations need to add penetration enhancers, such as dipropylene glycol methyl ether and other components). Rosemary volatile oil can dissolve the lipid layer of the insect exoskeleton or microbial cell membrane, destroying the integrity of the membrane structure, thereby promoting the penetration of other components. Gardenia fruit extract can bind to the microbial cell membrane protein, change the membrane permeability, and further improve the penetration of other components, improving the utilization rate. At the same time, gardenia fruit extract inhibits the synthesis of bacterial proteins and nucleic acids, and selamectin kills parasites by interfering with the conduction of nerve signals in the worm. The action mechanisms of the three cooperate with each other and enhance each other, reducing the drug resistance that may occur when using a single component, and jointly improving the insecticidal and antibacterial effects. Specific Embodiments

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] In the present invention, among the technically characterized descriptions in an open-ended manner, a closed technical solution composed of the listed features is included, and an open technical solution including the listed features is also included.

[0022] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0023] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0024] For the reagents or instruments used in the present invention, if the manufacturer is not specified, they are all conventional products that can be obtained through commercial purchase. The raw materials used in each comparative example and the raw materials used in the parallel experiments of each example are the same commercially available products unless otherwise specified.

[0025] Example 1 A pet topical selamectin drop, comprising the following components by weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 5% selamectin, 6% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0026] The preparation method of the rosemary volatile oil is as follows: Rosemary is crushed to 200 mesh to obtain rosemary powder. The rosemary powder is added to water, and steam distillation is carried out at 100 °C for 8 h to obtain an oil - water mixture. After standing, the aqueous phase is separated to obtain rosemary volatile oil. The mass ratio of the rosemary powder to water is 1:8.

[0027] The preparation method of the gardenia fruit extract is as follows: Gardenia fruits are crushed to 200 mesh to obtain gardenia fruit powder. The gardenia fruit powder, cellulase, β - glucanase, and xylanase are added to water, and enzymatic hydrolysis is carried out at 48 °C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the gardenia fruit powder, cellulase, β - glucanase, xylanase, and water is 1:0.02:0.008:0.001:8.

[0028] The enzymatic hydrolysate is ultrafiltered first with an ultrafiltration membrane with a molecular weight cut - off of 500 Da, then ultrafiltered with an ultrafiltration membrane with a molecular weight cut - off of 5000 Da, and dried to obtain the gardenia fruit extract.

[0029] The preparation method of the pet topical selamectin drop is characterized by comprising the following steps: The rosemary volatile oil, 1,2 - propanediol, and dimethyl sulfoxide are mixed evenly, and then butylated hydroxyanisole, dipotassium glycyrrhizinate, and selamectin are added and stirred evenly to obtain the pet topical selamectin drop.

[0030] Example 2 A topical selamectin drop for pets, comprising the following components in percentage by weight: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 1.5% rosemary volatile oil, 3% selamectin, 8.5% gardenia fruit extract, 10% 1,2-propanediol, and the balance dimethyl sulfoxide.

[0031] The preparation method of the rosemary volatile oil is as follows: crush rosemary to 200 mesh to obtain rosemary powder, add the rosemary powder to water, carry out steam distillation at 100 °C for 8 h to obtain an oil-water mixture, and after standing, separate the aqueous phase to obtain rosemary volatile oil. The mass ratio of the rosemary powder to water is 1:8.

[0032] The preparation method of the gardenia fruit extract is as follows: Crush gardenia fruit to 200 mesh to obtain gardenia fruit powder, add the gardenia fruit powder, cellulase, β-glucanase, and xylanase to water, and carry out enzymatic hydrolysis at 48 °C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the gardenia fruit powder, cellulase, β-glucanase, xylanase, and water is 1:0.02:0.008:0.001:8.

[0033] Ultrafilter the enzymatic hydrolysate first with an ultrafiltration membrane with a molecular weight cut-off of 500 Da, and then with an ultrafiltration membrane with a molecular weight cut-off of 5000 Da, and dry to obtain the gardenia fruit extract.

[0034] The preparation method of the topical selamectin drop for pets is characterized by comprising the following steps: uniformly mix the rosemary volatile oil, 1,2-propanediol, and dimethyl sulfoxide, and then add butylated hydroxyanisole, dipotassium glycyrrhizinate, selamectin, and selamectin, and stir evenly to obtain the topical selamectin drop for pets.

[0035] Example 3 A topical selamectin drop for pets, comprising the following components in percentage by weight: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 4% rosemary volatile oil, 6% selamectin, 3% gardenia fruit extract, 10% 1,2-propanediol, and the balance dimethyl sulfoxide.

[0036] The preparation method of the rosemary volatile oil is as follows: crush rosemary to 200 mesh to obtain rosemary powder, add the rosemary powder to water, carry out steam distillation at 100 °C for 8 h to obtain an oil-water mixture, and after standing, separate the aqueous phase to obtain rosemary volatile oil. The mass ratio of the rosemary powder to water is 1:8.

[0037] The preparation method of the gardenia fruit extract is as follows: The gardenia fruit is crushed to 200 mesh to obtain gardenia fruit powder. The gardenia fruit powder, cellulase, β-glucanase, and xylanase are added to water and enzymatically hydrolyzed at 48 °C for 5 h to obtain an enzymatically hydrolyzed solution; the mass ratio of the gardenia fruit powder, cellulase, β-glucanase, xylanase, and water is 1:0.02:0.008:0.001:8.

[0038] The enzymatically hydrolyzed solution is ultrafiltered first with an ultrafiltration membrane with a molecular weight cut-off of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 5000 Da, and dried to obtain a gardenia fruit extract.

[0039] The preparation method of the pet topical selamectin drops is characterized by comprising the following steps: mixing rosemary volatile oil, 1,2-propanediol, and dimethyl sulfoxide evenly, and then adding butylated hydroxyanisole, dipotassium glycyrrhizinate, selamectin, and selamectin, and stirring evenly to obtain the pet topical selamectin drops.

[0040] Example 4 A pet topical selamectin drop comprises the following components in weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 5% selamectin, 6% gardenia fruit extract, 10% 1,2-propanediol, and the balance dimethyl sulfoxide.

[0041] The preparation method of the rosemary volatile oil is as follows: the rosemary is crushed to 200 mesh to obtain rosemary powder. The rosemary powder is added to water and steam distilled at 100 °C for 8 h to obtain an oil-water mixture. After standing, the aqueous phase is separated to obtain rosemary volatile oil. The mass ratio of the rosemary powder to water is 1:8.

[0042] The preparation method of the gardenia fruit extract is as follows: The gardenia fruit is crushed to 200 mesh to obtain gardenia fruit powder. The gardenia fruit powder, cellulase, β-glucanase, and xylanase are added to water and enzymatically hydrolyzed at 48 °C for 5 h to obtain an enzymatically hydrolyzed solution; the mass ratio of the gardenia fruit powder, cellulase, β-glucanase, xylanase, and water is 1:0.01:0.002:0.005:8.

[0043] The enzymatically hydrolyzed solution is ultrafiltered first with an ultrafiltration membrane with a molecular weight cut-off of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 5000 Da, and dried to obtain a gardenia fruit extract.

[0044] The preparation method of the pet topical selamectin drops is characterized by comprising the following steps: mixing rosemary volatile oil, 1,2-propanediol, and dimethyl sulfoxide evenly, and then adding butylated hydroxyanisole, dipotassium glycyrrhizinate, selamectin, and selamectin, and stirring evenly to obtain the pet topical selamectin drops.

[0045] Comparative Example 1

[0046] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain rosemary volatile oil, and the others are the same.

[0047] A pet topical selamectin drop, comprising the following components in weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 5% selamectin, 6% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0048] Comparative Example 2

[0049] The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain selamectin, and the others are the same.

[0050] A pet topical drop, comprising the following components in weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 6% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0051] Comparative Example 3

[0052] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain gardenia fruit extract, and the others are the same.

[0053] A pet topical selamectin drop, comprising the following components in weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 5% selamectin, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0054] Comparative Example 4

[0055] The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain rosemary volatile oil (the total amount of rosemary volatile oil, selamectin, and gardenia fruit extract is the same), and the others are the same.

[0056] A pet topical selamectin drop, comprising the following components in weight percentage: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 5% selamectin, 6% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0057] Comparative Example 5

[0058] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain selamectin (the total amount of rosemary volatile oil, selamectin, and gardenia fruit extract is the same), and the others are the same.

[0059] A pet topical drop, comprising components in the following weight percentages: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 4.5% rosemary volatile oil, 8.5% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0060] Comparative Example 6

[0061] The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain gardenia fruit extract (the total amount of rosemary volatile oil, selamectin, and gardenia fruit extract is the same), and the others are the same.

[0062] A pet topical selamectin drop, comprising components in the following weight percentages: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 5% rosemary volatile oil, 8% selamectin, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0063] Comparative Example 7

[0064] The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses an equal amount of eucalyptus volatile oil to replace rosemary volatile oil, and the others are the same.

[0065] A pet topical selamectin drop, comprising components in the following weight percentages: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% eucalyptus volatile oil, 5% selamectin, 6% gardenia fruit extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0066] The preparation method of the eucalyptus volatile oil is as follows: The eucalyptus is crushed to 200 meshes to obtain eucalyptus powder. The eucalyptus powder is added to water, and steam distillation is carried out at 100 °C for 8 h to obtain an oil - water mixture. After standing, the aqueous phase is separated to obtain eucalyptus volatile oil. The mass ratio of the eucalyptus powder to water is 1:8.

[0067] Comparative Example 8

[0068] A pet topical selamectin drop, comprising components in the following weight percentages: 0.2% butylated hydroxyanisole, 1% dipotassium glycyrrhizinate, 2% rosemary volatile oil, 5% selamectin, 6% sophora flavescens extract, 10% 1,2 - propanediol, and the balance dimethyl sulfoxide.

[0069] The preparation method of the sophora flavescens extract is as follows: The sophora flavescens is crushed to 200 meshes to obtain sophora flavescens powder. The sophora flavescens powder, cellulase, β - glucanase, and xylanase are added to water, and enzymatic hydrolysis is carried out at 48 °C for 5 h to obtain an enzymatic hydrolysate. The mass ratio of the sophora flavescens powder, cellulase, β - glucanase, xylanase, and water is 1:0.01:0.002:0.005:8.

[0070] The enzymatic hydrolysate was ultrafiltered first with an ultrafiltration membrane having a molecular weight cut-off of 500 Da, then ultrafiltered with an ultrafiltration membrane having a molecular weight cut-off of 5000 Da, and dried to obtain the Sophora flavescens extract.

[0071] Comparative Example 9

[0072] The difference between Comparative Example 9 and Example 1 was that the preparation method of the Gardenia fruit extract in Comparative Example 9 was different from that in Example 1, and the others were the same.

[0073] The preparation method of the Gardenia fruit extract was as follows: The Gardenia fruit was ground to 200 meshes to obtain Gardenia fruit powder. The Gardenia fruit powder, pectinase, trypsin, and flavor protease were added to water and enzymatically hydrolyzed at 48 °C for 5 h to obtain an enzymatic hydrolysate; the mass ratio of the Gardenia fruit powder, pectinase, trypsin, flavor protease, and water was 1:0.01:0.002:0.005:8.

[0074] The enzymatic hydrolysate was ultrafiltered first with an ultrafiltration membrane having a molecular weight cut-off of 500 Da, then ultrafiltered with an ultrafiltration membrane having a molecular weight cut-off of 5000 Da, and dried to obtain the Gardenia fruit extract.

[0075] Test Example

[0076] 1. The acaricidal rate was detected according to NY / T1151.2-2006.

[0077] 2. The test was carried out with reference to Appendix C of GB 15979-2002 Hygienic Standard for Disposable Sanitary Products, and the test strain was Escherichia coli 8099.

[0078] Table 1

[0079] As can be seen from Table 1, the drops described in the present invention have excellent acaricidal and bacteriostatic effects and can effectively treat parasitic diseases.

[0080] By comparing Example 1 with Comparative Examples 1 to 6, it can be seen that the rosemary volatile oil, selamectin, and Gardenia fruit extract described in the present invention have a significant synergistic effect in antibacterial and acaricidal aspects. Under the synergistic effect of the three, the acaricidal effect is significantly improved. The absence of any one of them, or the replacement of any one of them with other components, will lead to a significant decrease in the acaricidal and bacteriostatic effects, and thus a significant decrease in the effect of treating parasitic diseases.

[0081] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A selamectin drop for external use on pets, characterized in that, It comprises components in the following weight percentages: 0.05 - 0.4% antioxidant, 0.5 - 1.2% dipotassium glycyrrhizinate, 1.5 - 4% rosemary volatile oil, 3 - 6% selamectin, 3 - 8.5% gardenia fruit extract, 5 - 20% 1,2 - propanediol, and the balance being solvent.

2. The selamectin drop for external use on pets according to claim 1, wherein, The antioxidant includes at least one of butylated hydroxyanisole and 2,6 - di - tert - butyl - p - cresol.

3. The selamectin drop for external use on pets according to claim 1, characterized in that, The solvent includes at least one of dimethyl sulfoxide, α - pyrrolidone, dimethylformamide, and ethanol.

4. The selamectin drop for external use on pets according to claim 1, wherein The preparation method of the rosemary volatile oil is as follows: adding rosemary powder into water, performing steam distillation to obtain an oil - water mixture, and separating the aqueous phase to obtain rosemary volatile oil.

5. The selamectin drops for external use on pets according to claim 4, wherein The particle size of the rosemary powder is 50 - 400 mesh; The mass ratio of the rosemary powder to water is 1:(4 - 10); The distillation time is 4 - 10 h.

6. The selamectin drop for external use on pets according to claim 1, wherein The preparation method of the gardenia fruit extract is as follows: Adding gardenia fruit powder, cellulase, β - glucanase, and xylanase into water, and performing enzymatic hydrolysis to obtain an enzymatic hydrolysate; Ultrafiltering the enzymatic hydrolysate first with an ultrafiltration membrane with a cut - off molecular weight of 500 Da, then ultrafiltering with an ultrafiltration membrane with a cut - off molecular weight of 5000 Da, and drying to obtain the gardenia fruit extract.

7. The selamectin drop for external use on pets according to claim 6, wherein, The mass ratio of the gardenia fruit powder, cellulase, β - glucanase, xylanase, and water is 1:(0.01 - 0.02):(0.002 - 0.008):(0.001 - 0.005):(4 - 10).

8. The selamectin drops for external use on pets according to claim 6, characterized in that, The particle size of the gardenia fruit powder is 50 - 400 mesh; The temperature of the enzymatic hydrolysis is 45 - 52 °C, and the time of the enzymatic hydrolysis is 2 - 6 h.

9. The preparation method of the selamectin drop for external use on pets according to any one of claims 1 to 8, characterized in that, It includes the following steps: Mixing the rosemary volatile oil, 1,2 - propanediol, and the solvent evenly, then adding the antioxidant, dipotassium glycyrrhizinate, and selamectin, and stirring evenly to obtain an external selamectin drop for pets.

10. Use of the selamectin drop for external use on pets according to any one of claims 1 to 8 in the preparation of a disinfectant for external use on pets, characterized in that, The external disinfection drug includes a pet anthelmintic drug, a pet trematodiasis drug, or a pet cestodiasis drug.

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