Injection device and microfluidic system

Abstract

The present invention discloses a sampling device and a microfluidic system, belonging to the field of microfluidics. The sampling device and microfluidic system of the present invention can pass a reagent from a sampling port into a distribution chamber, which then directly distributes the reagent to multiple distribution channels simultaneously. By limiting the dimensions of the distribution chamber and the distribution channels, the distribution chamber can evenly distribute the reagent to the multiple distribution channels, and the flow rate of the reagent in each distribution channel is consistent. Compared with the existing technology, this not only reduces the structural complexity of the sampling device, but also facilitates increasing the number of liquid separation stages of the sampling device to improve the production capacity of the microfluidic system, and also makes the particle size of the product produced by the microfluidic system more uniform.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a sample injection device and a microfluidic system. Background Art

[0002] Microfluidics, a technology that precisely manipulates fluids at the micron scale, allows for the flexible combination or large-scale integration of multiple functional units in chemical and biological experimental processes, such as sample preparation, reaction, separation, and detection, on a tiny platform. The micron-scale channel structure on a microfluidic chip significantly increases the area-to-volume ratio of the fluid, enabling efficient mass transfer, improving reaction efficiency, and making experiments more controllable. Furthermore, the integration of various functional units through microfluidics can avoid operational errors caused by complex processes. As a result, the application of microfluidics in the fields of microreactors and micro / nanomaterial preparation has been deeply explored and researched.

[0003] Since the channel size on the microfluidic chip is only at the micron level, the output that can be achieved by a single microfluidic chip is limited and cannot meet the needs of industrial mass production. Generally, the production capacity per unit time is increased by designing multiple parallel units on a single microfluidic chip. How to ensure the uniformity of the flow rate between parallel units is the key difficulty. At present, the step-by-step binary division method is generally adopted, that is, one is divided into two, two is divided into four, four is divided into eight, and so on, so as to achieve multiple units working in parallel. The distribution efficiency of this structure is low, and a single-stage liquid separation can only be divided into two units. If you want to increase the number of parallels, you can only increase the number of liquid separation stages, which means that the structural complexity of the liquid separation device increases step by step. Summary of the Invention

[0004] The object of the present invention is to provide a sample injection device and a microfluidic system, which not only reduces the structural complexity of the sample injection device but also makes the particle size of the prepared product more uniform.

[0005] To achieve the above objectives, the following technical solutions are provided:

[0006] Injection device, comprising:

[0007] A sample injection member, the sample injection member including a distribution chamber and a sample injection port connected to the distribution chamber, an injection surface provided on the outer side of the sample injection member, the injection surface provided with a plurality of distribution flow channels all connected to the distribution chamber, the plurality of distribution flow channels being spaced apart along a first direction;

[0008] Along the first direction, the length of the distribution cavity between two adjacent distribution channels is l d , the width of the distribution channel is w b ;

[0009] Along the second direction, the width of the distribution cavity is w d , the depth of the distribution channel is h b ;

[0010] Along the third direction, the depth of the distribution cavity is h d , the length of the distribution channel is l b ;

[0011] Along the first direction, h d unchanged, w d constant;

[0012] Along the third direction, h b unchanged, w b constant;

[0013] ;

[0014] The first direction, the second direction and the third direction are arranged at an angle to each other.

[0015] As an optimal technical solution for the sampling device, the sampling part includes a shell and a cover, the sampling surface is provided on the shell, the shell or the cover is provided with a groove, and the cover is connected to the shell to close the notch of the groove and form the distribution chamber.

[0016] As a preferred technical solution of the sample injection device, the shell is further provided with connecting channels arranged in a one-to-one correspondence with the plurality of distribution channels, and the distribution channels are connected with the distribution chamber through the corresponding connecting channels.

[0017] As a preferred technical solution of the sample injection device, the distribution chamber and the distribution flow channel are respectively located on two sides of the shell.

[0018] As a preferred technical solution of the sample injection device, the sample injection surface is further provided with a collection channel for communicating with a collection device, and the distribution cavity and the distribution channel are isolated from the collection channel.

[0019] As a preferred technical solution of the sample injection device, the sample injection device further includes a distribution seal provided in one-to-one correspondence with the plurality of distribution flow channels, and the distribution seal is sleeved on the outer side of the corresponding distribution flow channel;

[0020] The sample injection device further includes a collection seal provided in one-to-one correspondence with the plurality of collection flow channels, and the collection seal is sleeved on the outer side of the corresponding collection flow channel.

[0021] As a preferred technical solution of the sample injection device, the plurality of distribution seals are integrally formed; or the plurality of distribution seals are separately provided and fixedly connected;

[0022] The plurality of collecting seals are integrally formed; or, the plurality of collecting seals are separately provided and fixedly connected.

[0023] As a preferred technical solution of the sample injection device, the sample injection surface is provided with distribution avoidance grooves corresponding to the plurality of distribution seals, and the distribution seals are embedded in the distribution avoidance grooves;

[0024] The sample inlet surface is provided with a collection avoidance groove corresponding to the plurality of collection sealing members, and the collection sealing members are embedded in the collection avoidance groove.

[0025] As a preferred technical solution of the sample injection device, the sample injection device further includes a temperature regulating member, the temperature regulating member is used to regulate the temperature of the reagent in the distribution chamber and / or the distribution channel; and / or,

[0026] The sample injection device further comprises a heat-insulating component, which is sleeved on the outside of the sample injection component, and the sample injection surface is located outside the heat-insulating component.

[0027] As a preferred technical solution of the sample injection device, the first direction, the second direction and the third direction are perpendicular to each other.

[0028] In order to achieve the above-mentioned purpose, a microfluidic system is also provided, comprising at least one microfluidic unit arranged in parallel, the microfluidic unit comprising a microfluidic chip and at least one injection device as described in any of the above items, the microfluidic chip being arranged on the injection surface, the microfluidic chip comprising a plurality of channel units arranged corresponding to the plurality of distribution channels of the injection device, the channel unit comprising a plurality of microfluidic channels arranged at intervals along the third direction and isolated from each other, the distribution channels being connected to the plurality of microfluidic channels of the corresponding channel units.

[0029] As a preferred technical solution of the microfluidic system, along the third direction, the length of the distribution channel between two adjacent microfluidic channels is L d ;

[0030] Along the axial direction of the microfluidic channel, the length of the microfluidic channel is L b ;

[0031] The width of the microfluidic channel is W b ;

[0032] Along the second direction, the depth of the microfluidic channel is H b ;

[0033] Along the axial direction of the microfluidic channel, H b Unchanged, W b constant;

[0034] ;

[0035] The second direction, the width direction of the microfluidic channel and the axial direction of the microfluidic channel are perpendicular to each other.

[0036] As a preferred technical solution of the microfluidic system, the sample injection devices are provided with two, and the microfluidic chip includes two connecting surfaces, and the two connecting surfaces are respectively connected to the sample injection surfaces of the two sample injection devices;

[0037] The microfluidic channel includes a microfluidic outlet and two microfluidic inlets, the two microfluidic inlets are respectively located on the two connecting surfaces, and the microfluidic outlet is located on one of the connecting surfaces;

[0038] In the same channel unit, the two microfluidic inlets of the multiple microfluidic channels are respectively connected to the corresponding distribution channels of the two injection devices, and the microfluidic outlets of the multiple microfluidic channels are all used to communicate with the collection device.

[0039] As an optimal technical solution for the microfluidic system, the injection surface of one of the injection devices is also provided with at least one collecting flow channel corresponding to a plurality of channel units, and the plurality of microfluidic outlets of the channel unit are all connected to the corresponding collecting flow channel, and the collecting flow channel is connected to the collecting device.

[0040] As a preferred technical solution of the microfluidic system, the two injection devices of the same microfluidic unit are respectively a first injection device and a second injection device;

[0041] The microfluidic units are provided in plurality, the injection parts of the first injection devices of the plurality of microfluidic units are connected to form a total injection part, and the distribution chambers of the first injection devices of the plurality of microfluidic units are connected to form a total distribution chamber.

[0042] As a preferred technical solution of the microfluidic system, the main sample injection part includes a plurality of the sample injection surfaces, and the plurality of the sample injection surfaces are sequentially arranged on the outer peripheral side of the main sample injection part along the first circumferential direction.

[0043] As an optimal technical solution for the microfluidic system, the total distribution chamber includes a first distribution sub-chamber and a second distribution sub-chamber arranged in one-to-one correspondence with the multiple injection surfaces of the total injection part, the injection port and the multiple second distribution sub-chambers are all connected to the first distribution sub-chamber, and the multiple second distribution sub-chambers are connected in sequence along the first circumferential direction to form a total distribution sub-chamber with an annular structure.

[0044] As a preferred technical solution of the microfluidic system, the total sample injection unit includes:

[0045] a first shell, wherein the plurality of injection surfaces are sequentially arranged on the outer circumference of the first shell along the first circumferential direction;

[0046] The second shell is arranged in the first shell, and the main distribution cavity is formed between the first shell and the second shell.

[0047] As a preferred technical solution for the microfluidic system, along the third direction, one end of the second shell abuts against the inner wall of the first shell, and the first distribution sub-chamber is formed between the other end of the second shell and the inner wall of the first shell, and the total distribution sub-chamber is formed between the outer peripheral side of the second shell around the third direction and the inner wall of the first shell.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] The sample injection device and microfluidic system of the present invention can pass the reagent from the sample injection port into the distribution chamber, and then the distribution chamber directly distributes the reagent to multiple distribution channels at the same time. , so that the distribution chamber can evenly distribute the reagent to multiple distribution channels, and the flow rate of the reagent in each distribution channel is consistent. Compared with the existing technology, it not only reduces the structural complexity of the sampling device, but also facilitates increasing the number of liquid separation stages of the sampling device to improve the production capacity of the microfluidic system, and can also make the particle size of the product produced by the microfluidic system more uniform. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 This is a first structural schematic diagram of a microfluidic system in an embodiment of the present invention;

[0051] Figure 2 A second structural diagram of the microfluidic system according to an embodiment of the present invention;

[0052] Figure 3 Schematic diagram of the first structure of the first sample injection device in an embodiment of the present invention;

[0053] Figure 4 for Figure 3 The cross-sectional view of the AA surface;

[0054] Figure 5 This is a second structural schematic diagram of the first sample injection device in an embodiment of the present invention;

[0055] Figure 6 Schematic diagram of the first structure of the second sample injection device in an embodiment of the present invention;

[0056] Figure 7 for Figure 6 Cross-sectional view of the BB surface;

[0057] Figure 8 Schematic diagram of the structure of the first sample injection device and the microfluidic chip in an embodiment of the present invention;

[0058] Figure 9 for Figure 8 Enlarged view of point C;

[0059] Figure 10 Schematic diagram of a cross section of a microfluidic channel in an embodiment of the present invention;

[0060] Figure 11 Schematic diagram of the structure of the second sample injection device and the microfluidic chip in an embodiment of the present invention;

[0061] Figure 12 This is a third structural schematic diagram of the microfluidic system in an embodiment of the present invention;

[0062] Figure 13 is a cross-sectional view of the total sample injection component in an embodiment of the present invention;

[0063] Figure 14 Schematic diagram of the structure of the total sample feeding part in an embodiment of the present invention;

[0064] Figure 15 Schematic diagram of the second structure of the second sample injection device in an embodiment of the present invention;

[0065] Figure 16 A photo of droplets prepared in a microfluidic system in the prior art;

[0066] Figure 17 This is a photo of droplets prepared by a microfluidic system using a first-type sample injection device in an embodiment of the present invention;

[0067] Figure 18 This is a photo of droplets prepared by the microfluidic system using the second specification of the injection device in an embodiment of the present invention.

[0068] Reference numerals:

[0069] 1a, first sample injection device; 1b, second sample injection device; 11, sample injection member; 111, distribution chamber; 112, sample injection port; 113, sample injection surface; 1131, distribution flow channel; 1132, collection flow channel; 1133, sealing avoidance groove; 114, housing; 1141, groove; 1142, connecting channel; 115, cover; 116, collection channel; 12, sample injection connector; 13, collection connector; 141, groove seal; 142, flow channel seal; 15, temperature control Section; 16, insulation part; 11a, total sample injection part; 111a, total distribution chamber; 111a1, first distribution sub-chamber; 111a2, second distribution sub-chamber; 114a, first shell; 114a1, shell; 114a2, cover; 114a3, shell seal; 114b, second shell; 2, microfluidic chip; 211, microfluidic channel; 2111, microfluidic inlet; 2112, microfluidic outlet; 221, first connecting surface; 222, second connecting surface. DETAILED DESCRIPTION

[0070] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0071] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0072] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0073] In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, or are the orientation or position relationship in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0074] In the description of the present invention, it should be noted that, unless otherwise specified or limited, the terms "disposed" and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0075] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0076] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0077] Example 1

[0078] like Figures 1-11 As shown, this embodiment provides a sample injection device and a microfluidic system. The microfluidic system includes a sample injection device and a microfluidic chip 2 . The sample injection device is used to introduce reagents into the microfluidic channel 211 of the microfluidic chip 2 .

[0079] like Figure 1-Figure 5 As shown, the sampling device includes a sampling part 11, the sampling part 11 includes a distribution chamber 111 and an injection port 112 connected to the distribution chamber 111, an injection surface 113 is provided on the outside of the sampling part 11, and the sampling surface 113 is provided with a plurality of distribution channels 1131 all connected to the distribution chamber 111, and the plurality of distribution channels 1131 are arranged at intervals along the first direction.

[0080] Along the first direction, the length of the distribution cavity 111 between two adjacent distribution channels 1131 is l d , the width of the distribution channel 1131 is w b Along the second direction, the width of the distribution cavity 111 is w d , the depth of the distribution channel 1131 is h b Along the third direction, the depth of the distribution cavity 111 is h d , the length of the distribution channel 1131 is l b ; Along the first direction, h d unchanged, w d unchanged; along the third direction, h b unchanged, w b constant; .

[0081] The first direction, the second direction and the third direction are arranged at an angle to each other.

[0082] It should be noted that, along the first direction, the length of the distribution cavity 111 between two adjacent distribution channels 1131 is the length of the distribution cavity 111 between the axes of the two adjacent distribution channels 1131. It is understood that the axes of the distribution channels 1131 are parallel to the third direction.

[0083] In the sample injection device of this embodiment, only one sample injection port 112 is provided, which helps to ensure the consistency of the reagent flow rate in each distribution channel 1131.

[0084] The injection device of this embodiment adopts a single-stage liquid separation method, that is, after the reagent is introduced into the distribution chamber 111 from the injection port 112, the distribution chamber 111 directly distributes the reagent to multiple distribution channels 1131 at the same time. Compared with the existing technology, it not only greatly reduces the structural complexity of the injection device, but also effectively realizes the amplification of the fluid flux of the microfluidic chip 2, thereby improving the production capacity of the microfluidic system. , so that the distribution chamber 111 can evenly distribute the reagent to the multiple distribution channels 1131, and the flow rate of the reagent in each distribution channel 1131 is consistent, and the particle size of the product produced by the microfluidic system can be more uniform.

[0085] In this embodiment, the first direction, the second direction and the third direction are perpendicular to each other.

[0086] Specifically, the cross section of the distribution cavity 111 is rectangular, and the cross section of the distribution cavity 111 is perpendicular to the first direction. The cross section of the distribution channel 1131 is rectangular, and the cross section of the distribution channel 1131 is perpendicular to the third direction.

[0087] As an alternative, the angle between any two of the first, second, and third directions may be approximately 90°, or the angle between any two directions may be close to 90°. It should be noted that this embodiment does not limit the range of the angle between any two directions.

[0088] Alternatively, as Figure 4 and Figure 7As shown, the sample injection member 11 includes a housing 114 and a cover 115. The sample injection surface 113 is provided on the housing 114. The housing 114 is also provided with a groove 1141. The cover 115 is connected to the housing 114 to close the notch of the groove 1141 and form the distribution chamber 111. In other words, the cover 115 is covered with the notch of the groove 1141. By connecting the cover 115 to the housing 114, the notch of the groove 1141 can be closed to form the distribution chamber 111, thereby facilitating the processing of the distribution chamber 111 and facilitating the assembly of the cover 115 and the housing 114. It should be noted that the sample injection surface 113 is provided on the outer surface of the housing 114. The connection between the housing 114 and the cover 115 cannot block the sample injection surface 113, so that the microfluidic chip 2 can be placed on the sample injection surface 113. Of course, the groove 1141 may also be provided on the cover 115 , and the cover 115 is connected to the shell 114 so that the shell 114 closes the notch of the groove 1141 to form the distribution chamber 111 .

[0089] Exemplarily, the shell 114 and the cover 115 are connected by fasteners such as screws, which makes assembly and disassembly easy.

[0090] Optionally, the sample injection port 112 is used to connect to the reagent supply device, and the sample injection port 112 and the distribution channel 1131 are respectively arranged on both sides of the shell 114. That is to say, the sample injection port 112 can be arranged on any side of the sample injection part 11 where the distribution channel 1131 is not set, thereby avoiding interference between the microfluidic chip 2 and the sample injection port 112, and improving the operational convenience of the sample injection device.

[0091] Optionally, the injection port 112 is provided with an injection connector 12, and the injection port 112 is connected to the reagent supply device through the injection connector 12, which is more convenient to operate. Exemplarily, the injection connector 12 is connected to the injection port 112 by a thread, which is convenient to disassemble and assemble, and has good sealing performance.

[0092] Optionally, the sample injection device further includes a groove seal 141 corresponding to the groove 1141. The groove seal 141 is sleeved on the outside of the groove 1141 and sandwiched between the housing 114 and the cover 115, thereby improving the sealing of the distribution chamber 111. Exemplarily, the groove seal 141 is a sealing ring.

[0093] Alternatively, as Figure 3 As shown, the housing 114 is further provided with connecting channels 1142 corresponding to the plurality of distribution channels 1131. The distribution channels 1131 communicate with the distribution chamber 111 through the corresponding connecting channels 1142. Since the distribution channels 1131 are provided on the outer surface of the housing 114, the connecting channels 1142 connect the distribution channels 1131 and the distribution chamber 111, thereby ensuring the sealing of the distribution chamber 111 and facilitating the processing of the distribution chamber 111 and the distribution channels 1131.

[0094] Alternatively, as Figure 4 As shown, the distribution chamber 111 and the distribution channel 1131 are respectively located on both sides of the shell 114, that is, the groove 1141 and the distribution channel 1131 are respectively located on both sides of the shell 114, thereby avoiding interference between the cover 115 and the microfluidic chip 2 and facilitating assembly.

[0095] Alternatively, as Figure 6 and Figure 7 As shown, the sample inlet surface 113 is further provided with a collecting flow channel 1132 for communicating with a collecting device, and the distribution chamber 111 and the distribution flow channel 1131 are isolated from the collecting flow channel 1132 .

[0096] It should be noted that if Figure 8 and Figure 11 As shown, the microfluidic channel 211 of the microfluidic chip 2 includes a microfluidic inlet 2111 and a microfluidic outlet 2112. The distribution channel 1131 is used to communicate with the microfluidic inlet 2111 to introduce reagents into the microfluidic channel 211 through the microfluidic inlet 2111. The collection channel 1132 is used to communicate with the microfluidic outlet 2112 so that products formed in the microfluidic channel 211 can be discharged from the microfluidic outlet 2112 into the collection channel 1132 and finally collected by the collection device. The provision of the collection channel 1132 can improve the convenience of product collection, and the collection channel 1132 has a simple manufacturing process, which is conducive to reducing costs.

[0097] It is understood that the microfluidic chip 2 is generally provided with multiple microfluidic channels 211. Furthermore, multiple collecting channels 1132 are provided, so that the microfluidic outlets 2112 of the multiple microfluidic channels 211 of the microfluidic chip 2 are respectively connected to the multiple collecting channels 1132.

[0098] Alternatively, as Figure 6 As shown, the sampling device also includes a collection connector 13 arranged in a one-to-one correspondence with the collection flow channel 1132. The sampling component 11 is also provided with a collection channel 116 arranged in a one-to-one correspondence with the collection flow channel 1132. The collection connector 13 is installed at one end of the corresponding collection channel 116, and the other end of the collection channel 116 is connected to the corresponding collection flow channel 1132, and then connected to the collection device through the collection connector 13 to improve convenience.

[0099] Optionally, the sampling device also includes a distribution seal arranged in one-to-one correspondence with multiple distribution channels 1131, and the distribution seal is sleeved on the outside of the corresponding distribution channel 1131. After the sampling device and the microfluidic chip 2 are assembled, the sampling component 11 and the microfluidic chip 2 can clamp the distribution seal, which not only improves the sealing of the distribution channel 1131 and avoids leakage problems, but also facilitates assembly.

[0100] Optionally, the sampling device also includes a collection seal arranged in one-to-one correspondence with multiple collection channels 1132, and the collection seal is sleeved on the outside of the corresponding collection channel 1132. After the sampling device and the microfluidic chip 2 are assembled, the sampling part 11 and the microfluidic chip 2 can clamp the collection seal, which not only improves the sealing of the collection channel 1132 and avoids leakage problems, but also facilitates assembly.

[0101] Optionally, the multiple distribution seals are integrally formed; or, the multiple distribution seals are separately provided and fixedly connected. In other words, the multiple distribution seals are integrated, which facilitates assembly convenience and improves assembly efficiency. For example, the multiple distribution seals are integrally formed using an injection molding process; or, the multiple separately provided distribution seals are fixedly connected using bonding or other methods.

[0102] Optionally, the multiple collection seals are integrally formed; or, the multiple collection seals are separately provided and fixedly connected. In other words, the multiple collection seals are integrated, which facilitates assembly convenience and improves assembly efficiency. For example, the multiple collection seals are integrally formed using an injection molding process; or, the multiple separately provided collection seals are fixedly connected by bonding or other means.

[0103] In this embodiment, when the sample inlet surface 113 is provided with only the distribution flow channel 1131, the multiple distribution seals located on the sample inlet surface 113 are integrally formed or separately provided and fixedly connected to form a single flow channel seal 142. When the sample inlet surface 113 is provided with both the distribution flow channel 1131 and the collection flow channel 1132, the multiple distribution seals and the multiple collection seals located on the sample inlet surface 113 are integrally formed or separately provided and fixedly connected to form a single flow channel seal 142.

[0104] Optionally, the sample inlet surface 113 is provided with distribution avoidance grooves corresponding to the multiple distribution seals, and the distribution seals are embedded in the distribution avoidance grooves, thereby fixing the distribution seals, ensuring the installation stability of the distribution seals, and improving the sealing effect.

[0105] Optionally, the sample inlet surface 113 is provided with a collection avoidance groove corresponding to the multiple collection seals, and the collection seals are embedded in the collection avoidance groove, which can fix the collection seals, ensure the installation stability of the collection seals, and thus improve the sealing effect.

[0106] In this embodiment, the distribution avoidance groove and the collection avoidance groove are connected to form an integrated sealed avoidance groove 1133 .

[0107] Alternatively, as Figure 2As shown, the sample injection device further includes a temperature regulating member 15, which is used to regulate the temperature of the reagent in the distribution chamber 111 and / or the distribution channel 1131, thereby meeting the preparation requirements of different products.

[0108] Optionally, the sample injection part 11 is also provided with a heat preservation part 16, which is sleeved on the outside of the sample injection part 11, and the sample injection surface 113 is located outside the heat preservation part 16, thereby improving the heat preservation effect of the sample injection part 11 through the heat preservation part 16, ensuring the temperature stability of the reagent, and the heat preservation part 16 will not interfere with the microfluidic chip 2.

[0109] Exemplarily, the temperature regulating member 15 includes an electric heating plate, which is embedded in the sample injection member 11, and the distribution chamber 111 and the multiple distribution channels 1131 are arranged corresponding to the electric heating plate, so that the electric heating plate can simultaneously heat the reagents in the distribution chamber 111 and the multiple distribution channels 1131, thereby realizing precise temperature control of the reagents.

[0110] Furthermore, the thermal insulation part 16 is a shell-like structure with one end open and the other end closed. The thermal insulation part 16 is sleeved on the outside of the sample injection part 11, and the sample injection surface 113 and the opening are located on the same side of the sample injection part 11, thereby not interfering with the cooperation between the sample injection surface 113 and the microfluidic chip 2.

[0111] The microfluidic system of this embodiment includes at least one microfluidic unit arranged in parallel, the microfluidic unit includes a microfluidic chip 2 and at least one sampling device as described above, the microfluidic chip 2 is arranged on the sampling surface 113, the microfluidic chip 2 includes a plurality of channel units arranged corresponding to the plurality of distribution channels 1131 of the sampling device, the channel unit includes a plurality of microfluidic channels 211 arranged at intervals along a third direction and isolated from each other, and the distribution channels 1131 are connected to the plurality of microfluidic channels 211 of the corresponding channel units.

[0112] The microfluidic system of this embodiment, by applying the above-mentioned sample injection device, not only improves the production capacity of the microfluidic system, but also makes the particle size of the product produced by the microfluidic system more uniform.

[0113] Figure 10 is a schematic cross-sectional view of the microfluidic channel 211. The cross-sectional view of the microfluidic channel 211 is perpendicular to the axial direction of the microfluidic channel 211. Figure 10 As shown, along the third direction, the length of the distribution channel 1131 between two adjacent microfluidic channels 211 is L d Along the axial direction of the microfluidic channel 211, the length of the microfluidic channel 211 is L b The width of the microfluidic channel 211 is W b Along the second direction, the depth of the microfluidic channel 211 is H b Along the axial direction of the microfluidic channel 211, H bUnchanged, W b The second direction, the width direction of the microfluidic channel 211 and the axial direction of the microfluidic channel 211 are perpendicular to each other.

[0114] Further, By making , so that the distribution channel 1131 can evenly distribute the reagent to multiple microfluidic channels 211, and the flow rate of the reagent in each microfluidic channel 211 is consistent, thereby making the particle size of the product produced by the microfluidic system more uniform. It should be noted that the cross-section of the microfluidic channel 211 is rectangular; the cross-section of the microfluidic channel 211 is perpendicular to the axial direction of the microfluidic channel 211. Exemplarily, the axis of the microfluidic channel 211 is a curve. Of course, in other embodiments, the axis of the microfluidic channel 211 can also be a straight line, which is not limited here.

[0115] Alternatively, as Figure 1 As shown, there are two sampling devices, and the microfluidic chip 2 includes two connecting surfaces, which are respectively connected to the sampling surfaces 113 of the two sampling devices; the microfluidic channel 211 includes a microfluidic outlet 2112 and two microfluidic inlets 2111, the two microfluidic inlets 2111 are respectively located on the two connecting surfaces, and the microfluidic outlet 2112 is located on one of the connecting surfaces; in the same channel unit, the two microfluidic inlets 2111 of multiple microfluidic channels 211 are respectively connected to the corresponding distribution channels 1131 of the two sampling devices, and the microfluidic outlets 2112 of multiple microfluidic channels 211 are all used to communicate with the collection device. It can be understood that the microfluidic chip 2 is sandwiched between the two sampling devices. By introducing two different reagents into the microfluidic channel 211 through the two sampling devices, the flow rates of the two reagents in the microfluidic channel 211 can be made the same, thereby being able to prepare a product with uniform particle size, and the prepared product is then discharged from the microfluidic outlet 2112 and collected by the collection device.

[0116] Optionally, the injection surface 113 of one of the injection devices is provided with the above-mentioned collection flow channel 1132, that is, only the injection surface 113 of one of the injection devices is provided with the collection flow channel 1132, and the injection surface 113 of the other injection device does not need to be provided with the collection flow channel 1132, thereby facilitating the centralized collection of the products prepared by the microfluidic system.

[0117] Optionally, multiple distribution channels 1131 are arranged at equal intervals along the first direction, thereby improving the manufacturability of the sample injection member 11 and facilitating the processing of the distribution channels 1131. Of course, the multiple distribution channels 1131 can also be divided into multiple groups, with the multiple groups of distribution channels 1131 being arranged at equal intervals along the first direction, and each group of distribution channels 1131 including multiple distribution channels 1131 being arranged at equal intervals along the first direction. This embodiment does not limit the spacing between two adjacent groups of distribution channels 1131, nor does it limit the spacing between two adjacent distribution channels 1131.

[0118] Optionally, the plurality of collecting channels 1132 are arranged at equal intervals along the first direction. Of course, the plurality of collecting channels 1132 can also be divided into multiple groups, with the multiple groups of collecting channels 1132 being arranged at equal intervals along the first direction, and each group of collecting channels 1132 comprising multiple collecting channels 1132 being arranged at equal intervals along the first direction. This embodiment does not limit the spacing between two adjacent groups of collecting channels 1132 or the spacing between two adjacent collecting channels 1132.

[0119] It should be noted that the distribution channels 1131 and channel units can be arranged in a one-to-one correspondence, that is, one distribution channel 1131 is connected to the multiple microfluidic channels 211 in the corresponding channel unit, and the reagent is then distributed to the multiple microfluidic channels 211 in the corresponding channel unit through the one distribution channel 1131. Of course, it is also possible to make one distribution channel 1131 correspond to multiple channel units, that is, one distribution channel 1131 is connected to the multiple microfluidic channels 211 in the corresponding multiple channel units, and the reagent is then distributed to the multiple microfluidic channels 211 in the corresponding multiple channel units through the one distribution channel 1131.

[0120] Furthermore, a collection channel 1132 is provided corresponding to one or more channel units. In other words, a collection channel 1132 is connected to the multiple microfluidic outlets 2112 of a corresponding channel unit, thereby allowing the product formed by a channel unit to be discharged into the corresponding collection channel 1132; or, a collection channel 1132 is connected to the multiple microfluidic outlets 2112 of multiple channel units, thereby allowing the products formed by multiple channel units to be discharged into the corresponding collection channel 1132. All collection channels 1132 are connected to the collection device, and the products discharged from the multiple microfluidic channels 211 of the corresponding channel unit are initially collected through the collection channel 1132 and then transported to the collection device, thereby improving the convenience of product collection.

[0121] Specifically, on the same connection surface, the microfluidic inlets 2111 of the multiple microfluidic channels 211 of a channel unit are evenly spaced along the third direction to form a row of microfluidic inlets 2111. On the same connection surface, the microfluidic outlets 2112 of the multiple microfluidic channels 211 of a channel unit are evenly spaced along the third direction to form a row of microfluidic outlets 2112. Of course, on the same connection surface, the microfluidic inlets 2111 of the multiple microfluidic channels 211 of two or more channel units can also be evenly spaced along the third direction to form a row of microfluidic inlets 2111.

[0122] Furthermore, a distribution channel 1131 is connected to one or more rows of microfluidic channels 211 , and a collection channel 1132 is connected to one or more rows of microfluidic outlets 2112 .

[0123] It should be noted that, for the convenience of description, the two microfluidic inlets 2111 of the microfluidic channel 211 are respectively recorded as the first inlet and the second inlet, and the second inlet and the microfluidic outlet 2112 are located on the same connecting surface. b When L is the length of the microfluidic channel 211 between the second inlet and the microfluidic outlet 2112, d is the length of the distribution channel 1131 between two adjacent second inlets along the third direction. Specifically, the length of the distribution channel 1131 between two adjacent second inlets along the third direction is the distance between the axes of the two adjacent second inlets along the third direction. b When L is the length of the microfluidic channel 211 between the first inlet and the microfluidic outlet 2112, d It is the length of the distribution channel 1131 between two adjacent first inlets along the third direction. Specifically, the length of the distribution channel 1131 between two adjacent first inlets along the third direction is the distance between the axes of the two adjacent first inlets along the third direction.

[0124] like Figure 1 As shown, in a specific embodiment of the present invention, the microfluidic system includes a microfluidic unit, which includes a microfluidic chip 2 and two sampling devices, respectively denoted as a first sampling device 1a and a second sampling device 1b. The first sampling device 1a is used to dispense reagent A into the microfluidic chip 2, and the second sampling device 1b is used to dispense reagent B into the microfluidic core. The two connecting surfaces of the microfluidic chip 2 are divided into a first connecting surface 221 and a second connecting surface 222. The first connecting surface 221 and the second connecting surface 222 are respectively located on both sides of the thickness direction of the microfluidic chip 2. The first connecting surface 221 is connected to the sampling surface 113 of the first sampling device 1a, and the second connecting surface 222 is connected to the sampling surface 113 of the second sampling device 1b.

[0125] Furthermore, the microfluidic chip 2 includes twelve channel units, each channel unit includes twenty-five microfluidic channels 211, each microfluidic channel 211 includes two microfluidic inlets 2111 and one microfluidic outlet 2112, one of the microfluidic inlets 2111 is located on the first connecting surface 221 and is used to introduce reagent A, the other microfluidic inlet 2111 is located on the second connecting surface 222 and is used to introduce reagent B, and the microfluidic outlet 2112 is located on the second connecting surface 222 and is used to discharge the product formed in the microfluidic channel 211.

[0126] On the first connecting surface 221 , the twenty-five microfluidic inlets 2111 of one channel unit are arranged at equal intervals along the third direction to form a row of microfluidic inlets 2111 .

[0127] On the second connecting surface 222 , the fifty microfluidic inlets 2111 of the two channel units are evenly spaced along the third direction to form a row of microfluidic inlets 2111 , and the twenty-five microfluidic outlets 2112 of one channel unit are evenly spaced along the third direction to form a row of microfluidic outlets 2112 .

[0128] Furthermore, the first sample injection device 1a is provided with twelve distribution channels 1131, which are arranged in a one-to-one correspondence with and communicate with the twelve rows of microfluidic inlets 2111 on the first connection surface 221. Reagent A is introduced into the distribution chamber 111 through the injection port 112. After pre-filling the distribution chamber 111, reagent A is evenly distributed to the twelve distribution channels 1131. Then, reagent A is distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0129] The second sample injection device 1b is provided with six distribution channels 1131 and seven collection channels 1132. The six distribution channels 1131 are arranged in a one-to-one correspondence with and communicate with the six rows of microfluidic inlets 2111 on the second connection surface 222. Among the seven collection channels 1132, two of the collection channels 1132 are arranged in a one-to-one correspondence with and communicate with two rows of microfluidic outlets 2112 on the second connection surface 222. Among the remaining five collection channels 1132 and the remaining ten rows of microfluidic outlets 2112, one collection channel 1132 is arranged in a one-to-one correspondence with and communicates with two rows of microfluidic outlets 2112. Reagent B is introduced into the distribution chamber 111 through the sample injection port 112. After the distribution chamber 111 is pre-filled with reagent B, it is evenly distributed to the six distribution channels 1131. Then, reagent A is distributed by each distribution channel 1131 to the corresponding microfluidic channel 211.

[0130] Then, the product formed by the reagent A and the reagent B on the microfluidic chip 2 is discharged from the microfluidic outlet 2112 to the corresponding collecting channel 1132 , and then enters the collecting device through the collecting channel 1132 .

[0131] In summary, the injection device of this embodiment, without increasing the number of injection ports 112 and adopting a single-stage liquid separation method, realizes the parallel operation of three hundred microfluidic channels 211, and the flow rates of reagent A and reagent B in the three hundred microfluidic channels 211 are consistent, and the particle size of the prepared product is uniform.

[0132] Table 1 is a comparison of the results of different specifications of injection devices used for droplet preparation

[0133]

[0134] From Table 1, we can see that when When, such as Figure 17 and Figure 18 As shown, the product droplets formed in the microfluidic channel 211 have uniform sizes, indicating that the injection flow rate of the microfluidic channel 211 is uniform. When, such as Figure 16 As shown, the product droplets formed in the microfluidic channels 211 are of different sizes, indicating that the injection flow rates between different microfluidic channels 211 are uneven.

[0135] The microfluidic system of this embodiment can be used for the high-throughput preparation of gelatin microspheres. The gelatin solution is in a liquid state at an ambient temperature of 50°C-60°C and becomes solid when the temperature drops below room temperature. Therefore, a temperature adjustment member 15 is embedded in the sample injection unit 11 of the injection device to regulate the temperature of the gelatin solution. Furthermore, a heat preservation member 16 is provided on the outside of the sample injection unit 11. After the temperature of the gelatin solution is adjusted by the temperature adjustment member 15, the heat preservation member 16 can ensure the temperature stability of the gelatin droplets throughout the preparation process.

[0136] Illustratively, the process for high-throughput preparation of gelatin microspheres using the microfluidic system of this embodiment is as follows:

[0137] 1. Adjust the temperature of the temperature regulating element 15 to 60°C and stabilize it for 30 minutes before starting the preparation.

[0138] 2. The first sampling device 1a is used to distribute gelatin solution to the microfluidic chip 2. The gelatin solution enters the distribution chamber 111 from the sampling port 112 of the first sampling device 1a, pre-fills the distribution chamber 111, and is evenly distributed to the twelve distribution channels 1131. Then, the gelatin solution is evenly distributed into the multiple microfluidic channels 211 of the microfluidic chip 2 through each distribution channel 1131.

[0139] 3. The first sampling device 1a is used to distribute mineral oil containing a surfactant to the microfluidic chip 2. The mineral oil containing a surfactant enters the distribution chamber 111 from the sampling port 112 of the second sampling device 1b, pre-fills the distribution chamber 111, and is evenly distributed to the six distribution channels 1131. Then, the mineral oil containing a surfactant is evenly distributed through each distribution channel 1131 into the multiple microfluidic channels 211 of the microfluidic chip 2.

[0140] 4. On the microfluidic chip 2, mineral oil containing surfactant shears the gelatin solution to form gelatin droplets. The gelatin droplets are then discharged from the microfluidic outlet 2112 and collected in the collection channel 1132. They then enter the low-temperature collection pool through the collection connector 13 for online solidification to form gelatin microspheres.

[0141] In summary, the microfluidic system of this embodiment can increase the preparation throughput of gelatin microspheres from 2 μL / min-5 μL / min to 600 μL / min-1500 μL / min, while ensuring that the particle size of the gelatin microspheres is highly uniform.

[0142] Example 2

[0143] like Figure 12-15 As shown, the difference between this embodiment and embodiment 1 is that the microfluidic system is provided with multiple microfluidic units, the sample injection parts 11 of the first sample injection devices 1a of the multiple microfluidic units are connected to form a total sample injection part 11a, and the distribution chambers 111 of the first sample injection devices 1a of the multiple microfluidic units are connected to form a total distribution chamber 111a.

[0144] The microfluidic system of this embodiment can simultaneously distribute reagents to the distribution channels 1131 of the first injection devices 1a of multiple microfluidic units through a total distribution chamber 111a, which not only further simplifies the structure of the injection device, but also helps to improve the consistency of the reagent flow rate in the distribution channel 1131 of the first injection devices 1a of multiple microfluidic units, thereby making the particle size of the product produced by the microfluidic system more uniform.

[0145] Furthermore, if Figure 12-14 As shown, the main sample injection part 11a includes multiple sample injection surfaces 113, and the multiple sample injection surfaces 113 are sequentially arranged on the outer peripheral side of the main sample injection part 11a along the first circumferential direction, thereby facilitating the assembly of the sample injection surfaces 113 and the corresponding microfluidic chip 2, thereby improving assembly convenience.

[0146] It is understood that the multiple distribution channels 1131 on each injection surface 113 of the master injection member 11a are all connected to the master distribution chamber 111a, and the injection port 112 is also connected to the master distribution chamber 111a. The reagent introduced into the master distribution chamber 111a through a single injection port 112 can evenly enter the multiple distribution channels 1131 on each injection surface 113.

[0147] Optionally, the main distribution chamber 111a includes a first distribution sub-chamber 111a1 and second distribution sub-chambers 111a2, each corresponding to the multiple injection surfaces 113 of the main sample injection member 11a. The injection port 112 and the multiple second distribution sub-chambers 111a2 are both connected to the first distribution sub-chamber 111a1. The multiple second distribution sub-chambers 111a2 are sequentially connected along the first circumferential direction to form an annular main distribution sub-chamber. In other words, a single injection port 112 and a single first distribution sub-chamber 111a1 are provided, allowing the reagent to enter the first distribution sub-chamber 111a1 through the single injection port 112 and then enter the main distribution sub-chamber through the first distribution sub-chamber 111a1, which is beneficial for improving the uniformity of reagent distribution.

[0148] Optionally, the total sample injection part 11a includes a first shell 114a and a second shell 114b, and multiple sample injection surfaces 113 are arranged in sequence on the outer peripheral side of the first shell 114a along the first circumferential direction; the second shell 114b is arranged in the first shell 114a, and a total distribution chamber 111a is formed between the first shell 114a and the second shell 114b, thereby reducing the difficulty of manufacturing the total sample injection part 11a and facilitating the assembly of the microfluidic system.

[0149] Optionally, along the third direction, one end of the second shell 114b abuts the inner wall of the first shell 114a, and a first distribution sub-chamber 111a1 is formed between the other end of the second shell 114b and the inner wall of the first shell 114a. A main distribution sub-chamber is formed between the outer circumference of the second shell 114b around the third direction and the inner wall of the first shell 114a. Because the reagent exerts a certain pressure when entering the main distribution chamber 111a through the injection port 112, the above-described arrangement of the first and second shells 114a, 114b, not only facilitates the passage of the reagent into the distribution channels 1131 on each injection surface 113, but also avoids pressure loss in the reagent, thereby achieving energy conservation.

[0150] Optionally, the first shell 114a includes a shell portion 114a1 with two open ends and two cover portions 114a2. The two cover portions 114a2 are respectively covered on the two open ends in a one-to-one correspondence, thereby facilitating the assembly of the first shell 114a and the second shell 114b.

[0151] Optionally, a shell seal 114a3 is provided between the cover portion 114a2 and the shell portion 114a1 to improve the sealing performance.

[0152] In a specific embodiment of the present invention, Figure 14As shown, the main sample feed member 11a includes four sample feed surfaces 113. The main sample feed member 11a has a cubic structure, and the four sample feed surfaces 113 are four outer surfaces of the cubic structure that are sequentially connected along its circumference. Preferably, the first distribution sub-chamber 111a1 is located above the second shell 114b and communicates with the main distribution sub-chamber. The main distribution sub-chamber has an annular structure and is arranged around the second shell 114b.

[0153] In other embodiments, the number of the sample injection surfaces 113 of the total sample injection part 11 a may be two, three, five, or even more, which may be selected according to the number of the microfluidic chips 2 and is not limited here.

[0154] The working principle of the microfluidic system of this embodiment is:

[0155] For the total sample injection part 11a, reagent A is sequentially introduced into the first distribution sub-chamber 111a1 and the four second distribution sub-chambers 111a2 from the injection port 112. After the reagent A pre-fills the total distribution chamber 111a, it is evenly distributed to the twelve distribution channels 1131 on the four sample injection surfaces 113. Then, reagent A is distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0156] For each second injection device 1b, reagent B is introduced into the distribution chamber 111 through the injection port 112. After the distribution chamber 111 is pre-filled with reagent B, it is evenly distributed to the six distribution channels 1131. Then, reagent A is distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0157] Then, the product formed by the reagent A and the reagent B on the microfluidic chip 2 is discharged from the microfluidic outlet 2112 to the corresponding collecting channel 1132 , and then enters the collecting device through the collecting channel 1132 .

[0158] In summary, the microfluidic system of this embodiment realizes the parallel operation of four microfluidic chips 2 without increasing the number of sample injection ports 112 and adopting a single-stage liquid separation method. Each microfluidic chip 2 includes three hundred microfluidic channels 211, and the flow rates of reagent A and reagent B in the three hundred microfluidic channels 211 are consistent, and the particle size of the prepared product is uniform.

[0159] For example, the process of high-throughput preparation of nanoliposome drugs using the microfluidic system of this embodiment is as follows:

[0160] 1. Preparation of lipid solution: Accurately weigh 0.062g of DSPC (distearoylphosphatidylcholine) and 0.0152g of cholesterol and dissolve them in 39.45g of ethanol. Dissolve by ultrasonication. Filter the mixed solution through a 0.22μm organic filter. Preparation of aqueous solution: Dilute 10mL of 10× PBS (phosphate buffered saline) solution with purified water to 100mL of 1× PBS solution. Filter the solution through a 0.22μm aqueous filter.

[0161] 2. The lipid solution enters the main distribution chamber 111a from the injection port 112 of the main injection part 11a. After the lipid solution pre-fills the main distribution chamber 111a, it is evenly distributed to the distribution channel 1131 on each injection surface 113, and then distributed to the corresponding microfluidic channel 211 through each distribution channel 1131.

[0162] 3. The aqueous solution enters the corresponding distribution chamber 111 through the four sampling ports 112 of the second sampling device 1b. After the distribution chamber 111 is pre-filled with the aqueous solution, it is evenly distributed to the six distribution channels 1131. Then, the aqueous solution is distributed to the corresponding microfluidic channel 211 through each distribution channel 1131.

[0163] 4. The lipid solution and the aqueous solution are mixed in the microfluidic channel 211 and self-assembled to form nanoliposomes.

[0164] In summary, the microfluidic system of this embodiment can realize the simultaneous preparation of nanoliposome drugs using four microfluidic chips 2, and can increase the nanoliposome drug preparation throughput from the laboratory level (100 mL / min) to the pilot plant level (400 mL / min).

[0165] Note that the above are only preferred embodiments of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments and may include many other equivalent embodiments without departing from the concept of the present invention. The scope of the present invention is determined by the scope of the appended claims.

Claims

1. A sample injection device, characterized in that: include: A sample injection member, the sample injection member including a distribution chamber and a sample injection port connected to the distribution chamber, an injection surface provided on the outer side of the sample injection member, the injection surface provided with a plurality of distribution flow channels all connected to the distribution chamber, the plurality of distribution flow channels being spaced apart along a first direction; Along the first direction, the length of the distribution cavity between two adjacent distribution channels is l d , the width of the distribution channel is w b ; Along the second direction, the width of the distribution cavity is w d , the depth of the distribution channel is h b ; Along the third direction, the depth of the distribution cavity is h d , the length of the distribution channel is l b ; Along the first direction, h d unchanged, w d constant; Along the third direction, h b unchanged, w b constant; ； The first direction, the second direction and the third direction are arranged at an angle to each other.

2. The sample injection device according to claim 1, characterized in that The sample injection part includes a shell and a cover. The sample injection surface is provided on the shell. The shell or the cover is provided with a groove. The cover is connected to the shell to close the notch of the groove and form the distribution chamber.

3. The sample injection device according to claim 2, characterized in that The shell is further provided with connecting channels arranged in a one-to-one correspondence with the plurality of distribution flow channels, and the distribution flow channels are communicated with the distribution chamber through the corresponding connecting channels.

4. The sample injection device according to claim 2, characterized in that The distribution chamber and the distribution flow channel are respectively located on two sides of the shell.

5. The sample injection device according to claim 1, characterized in that The sample inlet surface is further provided with a collecting flow channel for communicating with a collecting device, and the distribution cavity and the distribution flow channel are isolated from the collecting flow channel.

6. The sample injection device according to claim 5, characterized in that The sample injection device further includes a distribution seal provided in one-to-one correspondence with the plurality of distribution flow channels, wherein the distribution seal is sleeved on the outer side of the corresponding distribution flow channel; The sample injection device further includes a collection seal provided in one-to-one correspondence with the plurality of collection flow channels, and the collection seal is sleeved on the outer side of the corresponding collection flow channel.

7. The sample injection device according to claim 6, characterized in that: The plurality of distribution seals are integrally formed; or, the plurality of distribution seals are separately provided and fixedly connected; The plurality of collecting seals are integrally formed; or, the plurality of collecting seals are separately provided and fixedly connected.

8. The sample injection device according to claim 6, characterized in that: The injection surface is provided with distribution avoidance grooves corresponding to the plurality of distribution seals, and the distribution seals are embedded in the distribution avoidance grooves; The sample inlet surface is provided with a collection avoidance groove corresponding to the plurality of collection sealing members, and the collection sealing members are embedded in the collection avoidance groove.

9. The sample injection device according to any one of claims 1 to 8, characterized in that: The sample injection device further comprises a temperature regulating member, wherein the temperature regulating member is used to regulate the temperature of the reagent in the distribution chamber and / or the distribution channel; and / or, The sample injection device further comprises a heat-insulating component, which is sleeved on the outside of the sample injection component, and the sample injection surface is located outside the heat-insulating component.

10. The sample injection device according to any one of claims 1 to 8, characterized in that: The first direction, the second direction and the third direction are perpendicular to each other.

11. A microfluidic system, characterized in that It comprises at least one microfluidic unit arranged in parallel, the microfluidic unit comprising a microfluidic chip and at least one injection device according to any one of claims 1 to 10, the microfluidic chip being arranged on the injection surface, the microfluidic chip comprising a plurality of channel units arranged corresponding to the plurality of distribution channels of the injection device, the channel unit comprising a plurality of microfluidic channels spaced apart and isolated from each other along the third direction, the distribution channels being connected to the plurality of microfluidic channels of the corresponding channel units.

12. The microfluidic system according to claim 11, characterized in that Along the third direction, the length of the distribution channel between two adjacent microfluidic channels is L d ; Along the axial direction of the microfluidic channel, the length of the microfluidic channel is L b ; The width of the microfluidic channel is W b ; Along the second direction, the depth of the microfluidic channel is H b ; Along the axial direction of the microfluidic channel, H b Unchanged, W b constant; ； The second direction, the width direction of the microfluidic channel and the axial direction of the microfluidic channel are perpendicular to each other.

13. The microfluidic system according to claim 12, characterized in that: There are two sample injection devices, and the microfluidic chip includes two connection surfaces, and the two connection surfaces are respectively connected to the injection surfaces of the two sample injection devices; The microfluidic channel includes a microfluidic outlet and two microfluidic inlets, the two microfluidic inlets are respectively located on the two connecting surfaces, and the microfluidic outlet is located on one of the connecting surfaces; In the same channel unit, the two microfluidic inlets of the multiple microfluidic channels are respectively connected to the corresponding distribution channels of the two injection devices, and the microfluidic outlets of the multiple microfluidic channels are all used to communicate with the collection device.

14. The microfluidic system according to claim 13, characterized in that The injection surface of one of the injection devices is also provided with at least one collecting flow channel corresponding to the multiple channel units, and the multiple microfluidic outlets of the channel units are all connected to the corresponding collecting flow channels, and the collecting flow channels are connected to the collecting device.

15. The microfluidic system according to claim 12, characterized in that: The two sampling devices of the same microfluidic unit are respectively a first sampling device and a second sampling device; The microfluidic units are provided in plurality, the injection parts of the first injection devices of the plurality of microfluidic units are connected to form a total injection part, and the distribution chambers of the first injection devices of the plurality of microfluidic units are connected to form a total distribution chamber.

16. The microfluidic system according to claim 15, characterized in that The main sample injection part includes a plurality of the sample injection surfaces, and the plurality of sample injection surfaces are sequentially arranged on the outer peripheral side of the main sample injection part along a first circumferential direction.

17. The microfluidic system according to claim 16, characterized in that The total distribution chamber includes a first distribution sub-chamber and a second distribution sub-chamber arranged in one-to-one correspondence with the multiple injection surfaces of the total injection part. The injection port and the multiple second distribution sub-chambers are all connected to the first distribution sub-chamber, and the multiple second distribution sub-chambers are connected in sequence along the first circumferential direction to form a total distribution sub-chamber with an annular structure.

18. The microfluidic system according to claim 17, characterized in that The total sample injection part includes: a first shell, wherein the plurality of injection surfaces are sequentially arranged on the outer circumference of the first shell along the first circumferential direction; The second shell is arranged in the first shell, and the main distribution cavity is formed between the first shell and the second shell.

19. The microfluidic system according to claim 18, characterized in that Along the third direction, one end of the second shell abuts against the inner wall of the first shell, the first distribution sub-cavity is formed between the other end of the second shell and the inner wall of the first shell, and the total distribution sub-cavity is formed between the outer peripheral side of the second shell around the third direction and the inner wall of the first shell.

Images