Production and manufacturing device for enzyme digestive drugs

Abstract

The invention discloses an enzyme digestion-aiding medicine production and manufacturing device, and relates to the technical field of biological medicine manufacturing, the enzyme digestion-aiding medicine production and manufacturing device comprises a biological culture tank, the biological culture tank is composed of a tank cover and a tank body, a unique temperature control type stirring paddle is arranged in the tank body, and a first U-shaped pipe and a second U-shaped pipe in the temperature control type stirring paddle are crossed and are different in width; spiral button tubes at the two ends of the first U-shaped tube and the second U-shaped tube can achieve efficient stirring in the horizontal and vertical directions, and stirring is facilitated; above the tank body, the quick connector is driven by the rotary executing element, and the first circular connecting column is detachably connected with the quick connector, so that the maintenance is convenient; the quick connector is connected with the constant-temperature circulator through the double-shaft rotating connector to realize temperature regulation and control; besides, a guide seat surrounds a semi-elliptical guide groove in a quick connector to be matched with a rotating roller, so that the temperature control type stirring paddle can reciprocate up and down during rotation, the stirring dimension is increased, the mixing effect is improved, a higher-quality environment is provided for culture of enzyme microorganisms, and high-efficiency production of enzyme digestion-aiding drugs is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceutical manufacturing, and particularly relates to a production and manufacturing device for enzyme-based digestive drugs. Background Art

[0002] In the field of biopharmaceutical manufacturing, the production of enzyme-based digestive drugs is a complex and delicate process; first, enzyme-producing microbial strains with specific functions need to be screened, and the performance of these strains directly determines the quality and efficacy of the final product; subsequently, the screened strains are inoculated into a suitable culture medium and cultured in a bioreactor; during the culture process, precise temperature, humidity, nutrient components, and gas environment conditions need to be provided for the microorganisms to promote their growth, reproduction, and metabolism, thereby producing the required enzyme substances; next, subsequent treatments such as separation and purification are performed on the culture medium containing enzymes to remove impurities and concentrate the active ingredients, and finally, enzyme-based digestive drugs meeting the quality standards are produced; however, in this production process, a series of problems urgently need to be solved in the current production and manufacturing devices for many enzyme-based digestive drugs.

[0003] First, most existing devices rely only on simple paddle blades or stirring rods for stirring, and can only achieve limited mixing in the horizontal direction, which leads to uneven distribution of the enzyme-containing culture medium in the tank, and the enzyme-producing microorganisms in some areas cannot fully contact the nutrients, greatly limiting the growth and reproduction speed of the microorganisms, and thus affecting the yield and quality of the drugs; during the entire culture process, the mixing of the enzyme-containing culture medium in the vertical direction is particularly important; however, the stirring paddles of traditional devices lack the function of reciprocating up and down, resulting in insufficient mixing of the culture medium at different depths in the tank, uneven distribution of temperature and nutrients, and seriously affecting the stability of the growth environment of the enzyme-producing microorganisms; second, many existing devices only use external heating or cooling methods to control the temperature of the tank, and this single temperature control method is difficult to ensure that the temperature of the enzyme-containing culture medium in the tank is uniform, and local overheating or overcooling is likely to occur, which has a negative impact on the activity of the enzyme-producing microorganisms; in view of this, this article proposes a production and manufacturing device for enzyme-based digestive drugs. Summary of the Invention

[0004] The main purpose of the present invention is to provide a production and manufacturing device for enzyme-based digestive drugs, which can effectively solve the problems in the background art.

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] An enzyme digestive aid production and manufacturing device, including a biological culture tank, the biological culture tank includes a tank body and a tank cover, the tank cover is sealed and installed above the opening of the inner cavity of the tank body, a temperature-controlled stirring paddle is rotatably installed in the tank body, the temperature-controlled stirring paddle includes a first circular connecting column, a first U-shaped tube and a second U-shaped tube, four mutually parallel first connecting holes are arranged in the first circular connecting column, the outer wall of the first circular connecting column is rotatably installed in a closed manner on the central axis of the tank cover, the second U-shaped tube is cross-arranged above the first U-shaped tube, the outer width of the first U-shaped tube is greater than the outer width of the second U-shaped tube, two mutually symmetrical spiral tubes are fixedly communicated above both ends of the first U-shaped tube and the second U-shaped tube, the tops of the four spiral tubes are fixedly communicated below the four first connecting holes in the first circular connecting column, and the cross-sections of the first U-shaped tube, the second U-shaped tube and the spiral tubes are all flat;

[0007] A quick connector and a rotary actuator for driving the quick connector to rotate are coaxially and fixedly installed above the tank body, the first circular connecting column is detachably connected to the lower end of the quick connector, the top end of the quick connector is rotatably connected to a double-axis rotary joint, the quick connector is connected to a constant temperature circulator through the double-axis rotary joint, the quick connector includes a second circular connecting column, and a roller is rotatably installed on one side of the second circular connecting column;

[0008] A guide seat is arranged around the quick connector for guiding the quick connector and the connected temperature-controlled stirring paddle to move up and down reciprocally while rotating. The guide seat includes two semi-cylindrical plate blocks that are symmetrically joined together to form the entire cylinder and a fixed seat. The second circular connecting column is coaxially rotatably installed between the two semi-cylindrical plate blocks. The two semi-cylindrical plate blocks are fixedly installed above the tank cover through the fixed seat. And semi-elliptical guide grooves are provided on the inner walls of the two semi-cylindrical plate blocks. The two ends of the two semi-elliptical guide grooves are communicated with each other. The left end of the semi-elliptical guide groove is higher than the right end. The end of the roller is rollingly connected in the two semi-elliptical guide grooves.

[0009] Preferably, the biaxial rotary joint includes a second fixing nut, a circular connecting cylinder, a sealing cover and a fixing pressing plate. The inner circle of the upper end of the second fixing nut is telescopically installed on the outer periphery of the lower end of the circular connecting cylinder. Four third connecting holes are provided at the lower end of the circular connecting cylinder. A second positioning groove is communicated with the outer periphery of the lower end of the third connecting hole. Four sealing gaskets at the upper end of the second circular connecting column are respectively positioned and fitted in the four second positioning grooves. An inlet pipe is coaxially and fixedly connected in the circular connecting cylinder. The inlet pipe is communicated with the two third connecting holes. The inner cavities of the other two third connecting holes and the inner cavity between the inner circle of the circular connecting cylinder and the outer circle of the inlet pipe are communicated with each other. The outer circle of the sealing cover is rotatably installed on the outer periphery of the opening of the circular connecting cylinder through a first bearing. The inner circle of the sealing cover is rotatably installed on the outer periphery of the inlet pipe through a second bearing. A combined sealing piece is fitted on the inner top surface of the sealing cover. The outer diameter of the combined sealing piece is the same as the inner diameter of the circular connecting cylinder. The inner diameter of the combined sealing piece is the same as the outer diameter of the inlet pipe. The fixing pressing plate is press-fitted on the upper surface of the sealing cover. A first connecting pipe is fixedly installed at the center of the fixing pressing plate. The lower end of the first connecting pipe is communicated with the inner cavity of the inlet pipe. Two second connecting pipes are fixedly connected to both sides of the fixing pressing plate. The lower ends of the second connecting pipes are hermetically passed through the sealing cover and the combined sealing piece and are threadedly connected with nuts. A second sealing rubber sheet is arranged between the sealing cover and the fixing pressing plate.

[0010] Preferably, a support frame is connected below the constant temperature circulator and the tank body. A temperature sensor for measuring the internal temperature of the tank body is fixedly installed on the tank cover. The constant temperature circulator includes an electric water heater, a cold water tank, a constant temperature mixing valve, a circulation water pump and a flow dividing valve. The electric water heater and the cold water tank are fixedly installed on the support frame. The constant temperature mixing valve is fixedly installed between the electric water heater and the cold water tank. Two water inlets of the constant temperature mixing valve are respectively communicated with the water outlets of the electric water heater and the cold water tank. The water outlet of the constant temperature mixing valve is fixedly communicated with the water inlet of the circulation water pump. The circulation water pump is fixedly installed above the electric water heater. The water outlet of the circulation water pump is fixedly communicated with the water inlet of the flow dividing valve. One water outlet of the flow dividing valve is communicated with the first connecting pipe.

[0011] Preferably, a first positioning groove is communicated and arranged around the top ends of the four first connection holes in the first circular connection column. Threaded connection heads are fixedly installed at both ends of the second circular connection column. Four mutually parallel second connection holes are arranged in the second circular connection column. And positioning cylinders are fixedly communicated at both ends of the second connection holes in the second circular connection column. A sealing gasket is fixedly connected to the outer end of the positioning cylinder. The four second connection holes in the second circular connection column respectively correspond to the four first connection holes in the first circular connection column. And a first fixing nut is telescopically installed around the top end of the first circular connection column. The inner ring of the first fixing nut is threadedly installed on the threaded connection head at the lower end of the second circular connection column.

[0012] Preferably, the combined sealing sheet is composed of two stainless steel sheets and two first sealing rubber sheets. And the two stainless steel sheets and the two first sealing rubber sheets are cross-overlapped and adhesively connected. One of the upper first sealing rubber sheets is clamped and connected between the stainless steel sheet and the sealing cover. The lower surface of the lower stainless steel sheet is pressed against the nut.

[0013] Preferably, the rotary actuating element is composed of a stirring motor, a reducer and a spline gear. The casings of the stirring motor and the reducer are both fixedly connected to the tank cover. And the output end of the rotor in the stirring motor is connected to the spline gear through the reducer. A lifting gear is coaxially connected to the top end of the second circular connection column. The outer rings of the lifting gear and the spline gear are meshed with each other. And the outer walls of the lifting gear and the spline gear are slidably connected vertically.

[0014] Preferably, a temperature control interlayer is arranged on the outer layer of the tank body. One water outlet of the flow dividing valve is connected to the water inlet at the lower end of the temperature control interlayer. The water outlet at the upper end of the temperature control interlayer and the water outlets of the two second connecting pipes are both connected to the water inlet of the electric water heater.

[0015] Preferably, a flow guiding ring is fitted and slidably installed on the inner wall of the tank body. The flow guiding ring is arranged around the temperature control type stirring paddle. The flow guiding ring includes two coaxially arranged rings, two arc-shaped connecting plates and a plurality of arc-shaped flow guiding plates. The two arc-shaped connecting plates are symmetrically connected to both sides of the two rings respectively. The plurality of arc-shaped flow guiding plates are symmetrically installed on the inner walls of the two arc-shaped connecting plates respectively. The arc-shaped connecting plates are inclined.

[0016] Preferably, an inert gas high-pressure tank is fixedly installed on one side of the support frame. A pressure sensor for measuring the internal pressure of the tank body is fixedly installed on the tank cover. A gas guide pipe is fixedly communicated with the tank cover. A two-position three-way electromagnetic valve is fixedly installed above the gas guide pipe. The air inlet of the two-position three-way electromagnetic valve is communicated with the air outlet at the top end of the inert gas high-pressure tank. The air outlet of the two-position three-way electromagnetic valve is fixedly communicated with the air outlet of the gas guide pipe.

[0017] Preferably, a control cabinet is fixedly installed on one side of the support frame, and the control cabinet is arranged below the constant temperature circulator.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. Efficient stirring and mixing:

[0020] Unique stirring structure: The temperature-controlled stirring paddle is arranged with the first U-shaped tube and the second U-shaped tube crossed and with different widths, and is matched with the twist-shaped spiral tubes symmetrically arranged above both ends. It can not only stir enzyme culture solutions in different diameter ranges, but also achieve mixing in the horizontal and vertical directions, making the culture solution mix more evenly and greatly improving the mixing effect.

[0021] Multi-dimensional stirring movement: Through a series of outputs and transmissions of the guide seat, the roller in the quick connector, the lifting gear and the rotating execution element, the temperature-controlled stirring paddle can move up and down reciprocally while rotating, increasing the stirring dimension. Coupled with the guiding of the rotation direction of the enzyme culture solution by the arc-shaped guide plate in the flow guiding ring, the stirring efficiency is further improved to ensure full contact between the enzyme microorganisms and the culture solution.

[0022] 2. Precise temperature control:

[0023] Internal and external collaborative temperature control: The temperature-controlled stirring paddle and the temperature-adjusting interlayer cooperate with each other to control the temperature of the enzyme culture solution inside the tank from the inside and outside respectively, realizing more precise and efficient temperature maintenance and creating a suitable growth environment for the enzyme microorganisms.

[0024] Intelligent temperature control: The temperature sensor monitors the temperature in real time and feeds back the signal to the control cabinet. The control cabinet controls each component in the constant temperature circulator accordingly to adjust the temperature and flow rate of the circulating water, realizing precise control of the temperature inside the tank.

[0025] 3. Stable air pressure regulation: The pressure sensor monitors the pressure inside the tank in real time, transmits the signal to the control cabinet, and the control cabinet controls the two-way three-way solenoid valve to realize precise control of the inert gas entering and leaving the tank, maintaining the stable air pressure inside the tank and ensuring the stability of the enzyme microorganism culture environment.

[0026] 4. Good maintainability: The top of the temperature-controlled stirring paddle is detachably installed below the quick connector, which is convenient for cleaning and component replacement of the temperature-controlled stirring paddle, reduces the maintenance cost, and improves the service life of the temperature-controlled stirring paddle.

[0027] 5. Reliable sealing performance: The double-axis rotary joint adopts multiple sealing structures such as combined sealing pieces and second sealing rubber sheets. While ensuring the normal circulation of circulating water, it effectively prevents leakage. Especially when the water pressure changes, it can adaptively enhance the sealing effect to ensure the stable operation of this enzyme digestive medicine production and manufacturing device. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the overall structural schematic diagram of the present invention;

[0029] Figure 2 is the present invention Figure 1 The enlarged view of circle A in;

[0030] Figure 3 is the structural schematic diagram of the bioreactor in the present invention;

[0031] Figure 4 is the cross-sectional view of the bioreactor in the present invention;

[0032] Figure 5 is the partial structural schematic diagram of the bioreactor in the present invention;

[0033] Figure 6 is the structural schematic diagram of the temperature-controlled stirring paddle in the present invention;

[0034] Figure 7 is the connection schematic diagram of the second U-shaped tube and the spiral tube in the present invention;

[0035] Figure 8 is the structural schematic diagram of the flow guide ring in the present invention;

[0036] Figure 9 is the exploded view of the quick connector, double-axis rotary joint, and guide seat in the present invention;

[0037] Figure 10 is the structural schematic diagram of the quick connector in the present invention;

[0038] Figure 11 is the structural schematic diagram of the double-axis rotary joint in the present invention;

[0039] Figure 12 is the exploded view of the double-axis rotary joint in the present invention;

[0040] Figure 13 is the structural schematic diagram of the circular connecting cylinder in the present invention;

[0041] Figure 14 is the cross-sectional view of the double-axis rotary joint in the present invention;

[0042] Figure 15 is the present invention Figure 1 The enlarged view of circle B in.

[0043] In the figure: 1, support frame; 2, control cabinet; 3, biological culture tank; 31, tank body; 311, temperature control interlayer; 32, tank cover; 33, temperature control type stirring paddle; 331, first circular connecting column; 332, first fixing nut; 333, first connecting hole; 334, first U-shaped tube; 335, second U-shaped tube; 336, spiral twisting tube; 337, first positioning groove; 34, quick connector; 341, second circular connecting column; 342, roller; 343, lifting gear; 344, threaded connector; 345, positioning cylinder; 346, gasket; 347, second connecting hole; 35, double-axis rotary joint; 351, second fixing nut; 352, circular connecting cylinder; 353, third connecting hole; 354, sealing cover; 355, fixing pressing plate; 356, first sealing rubber sheet; 357, stainless steel sheet; 358, inlet pipe; 359, second sealing rubber sheet; 36, flow guiding ring; 361, circular ring; 362, arc connecting plate; 363, arc flow guiding plate; 37, guiding seat; 371, semi-cylindrical plate; 372, fixing seat; 373, semi-elliptical guiding groove; 38, temperature sensor; 39, two-position three-way solenoid valve; 4, inert gas high-pressure tank; 5, constant temperature circulator; 51, electric water heater; 52, cold water tank; 53, constant temperature mixing valve; 54, circulating water pump; 55, flow dividing valve; 6, rotary actuator; 61, stirring motor; 62, reducer; 63, spline gear. Detailed implementation manners

[0044] In order to make the technical means, creative features, achieved purposes and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific implementation manners.

[0045] As Figures 1 - 15 shown, an enzyme digestive aid production and manufacturing device includes a biological culture tank 3, whose main function is to culture enzyme microorganisms. The biological culture tank 3 is composed of a tank body 31 and a tank cover 32. The tank cover 32 is hermetically installed above the opening of the inner cavity of the tank body 31 to ensure the tightness of the culture environment.

[0046] Refer to Figure 4 、 Figure 5 、 Figure 6 and Figure 7As shown in the figure, a temperature-controlled stirring paddle 33 is rotatably installed inside the tank body 31. The temperature-controlled stirring paddle 33 includes a first circular connecting column 331, a first U-shaped tube 334, and a second U-shaped tube 335. Four mutually parallel first connecting holes 333 are provided inside the first circular connecting column 331. The outer wall of the first circular connecting column 331 is rotatably installed in a closed manner on the central axis of the tank cover 32. The second U-shaped tube 335 is cross-set above the first U-shaped tube 334, and the outer width of the first U-shaped tube 334 is greater than the outer width of the second U-shaped tube 335. This design can stir enzyme culture solutions within different diameter ranges. Circulating water can be introduced into the temperature-controlled stirring paddle 33 to regulate the temperature of the enzyme culture solution inside the tank body 31 from the inside to the outside.

[0047] Reference Figure 6 and Figure 7 As shown in the figure, two mutually symmetric spiral twist tubes 336 are fixedly connected and communicated above both ends of the first U-shaped tube 334 and the second U-shaped tube 335. The spiral twist tube 336 is in the shape of a twist. When the first U-shaped tube 334 or the second U-shaped tube 335 rotates inside the tank body 31, it can not only swing the enzyme culture solution outward, but also use the spiral inclined outer wall of the spiral twist tube 336 to stir the enzyme culture solution upward or downward. Since the two spiral twist tubes 336 above both ends of the first U-shaped tube 334 and the second U-shaped tube 335 are mutually symmetric, while one spiral twist tube 336 stirs the enzyme culture solution downward obliquely, the other spiral twist tube 336 stirs the enzyme culture solution upward obliquely, thereby realizing the mixing of the enzyme culture solution inside the tank body 31 in the horizontal and vertical directions.

[0048] Reference Figure 6 As shown in the figure, the tops of the four spiral twist tubes 336 are fixedly connected and communicated below the four first connecting holes 333 in the first circular connecting column 331. Moreover, the cross-sections of the first U-shaped tube 334, the second U-shaped tube 335, and the spiral twist tube 336 are all flat. At the same time, the width of the lower bottom surface of the first U-shaped tube 334 and the second U-shaped tube 335 is greater than the height. In this way, when the first U-shaped tube 334 and the second U-shaped tube 335 rise and fall, it is easier to drive the enzyme culture solution inside them to stir up and down.

[0049] Reference Figure 4 and Figure 8As shown, a flow guiding ring 36 is fitted and slidably mounted on the inner wall of the tank body 31. The flow guiding ring 36 is arranged around the temperature-controlled stirring paddle 33. The flow guiding ring 36 includes two coaxially arranged rings 361, two arc-shaped connecting plates 362 and a plurality of arc-shaped flow guiding plates 363. The two arc-shaped connecting plates 362 are symmetrically connected to both sides of the two rings 361 respectively. The plurality of arc-shaped flow guiding plates 363 are symmetrically mounted on the inner walls of the two arc-shaped connecting plates 362 respectively, and the arc-shaped connecting plates 362 are inclined. The plurality of arc-shaped flow guiding plates 363 on the flow guiding ring 36 mainly guide the enzymic culture solution flowing in a stirring and rotating manner to rotate upward or downward along the plurality of arc-shaped flow guiding plates 363 obliquely.

[0050] Reference Figure 4 As shown, a temperature control interlayer 311 is arranged on the outer layer of the tank body 31. Circulating water is introduced into the temperature control interlayer 311 for temperature control of the enzymic culture solution inside the tank body 31 from the outside to the inside, and it cooperates with the temperature-controlled stirring paddle 33.

[0051] Reference Figure 1 and Figure 3 As shown, a quick connector 34 and a rotary actuator 6 for driving the quick connector 34 to rotate are coaxially and fixedly mounted above the tank body 31. The top end of the temperature-controlled stirring paddle 33 is detachably mounted below the quick connector 34 for easy cleaning and replacement. Among them, the first circular connecting column 331 is detachably connected to the lower end of the quick connector 34. A double-axis rotary joint 35 is rotatably connected to the top end of the quick connector 34. The quick connector 34 is connected to a constant temperature circulator 5 through the double-axis rotary joint 35. The quick connector 34 includes a second circular connecting column 341, and a roller 342 is rotatably mounted on one side of the second circular connecting column 341.

[0052] Reference Figure 3 and Figure 5 As shown, the rotary actuator 6 is composed of a stirring motor 61, a speed reducer 62 and a spline gear 63. The casings of the stirring motor 61 and the speed reducer 62 are both fixedly connected to the tank cover 32. The output end of the rotor in the stirring motor 61 is connected to each other through the speed reducer 62 and the spline gear 63. The top end of the second circular connecting column 341 is coaxially connected with a lifting gear 343. The outer circles of the lifting gear 343 and the spline gear 63 are meshed with each other, and the outer walls of the lifting gear 343 and the spline gear 63 are vertically slidably connected. Thus, the output end of the rotor in the stirring motor 61 can drive the quick connector 34 to rotate through the speed reducer 62, the spline gear 63 and the lifting gear 343, and then drive the temperature-controlled stirring paddle 33 to rotate to stir the enzymic culture solution in the tank body 31.

[0053] Reference Figure 5 and Figure 9As shown, a guide seat 37 is provided around the quick connector 34 for guiding the quick connector 34 and the connected temperature-controlled stirring paddle 33 to perform reciprocating up and down movements while rotating. The guide seat 37 includes two semi-cylindrical plate blocks 371 that are symmetrically combined into the whole cylinder and a fixed seat 372. The second circular connecting column 341 is coaxially and rotatably installed between the two semi-cylindrical plate blocks 371. The two semi-cylindrical plate blocks 371 are fixedly installed above the tank cover 32 through the fixed seat 372. Semi-elliptical guide grooves 373 are provided on the inner walls of the two semi-cylindrical plate blocks 371. The two ends of the two semi-elliptical guide grooves 373 communicate with each other, and the left end of the semi-elliptical guide groove 373 is higher than the right end. The end of the roller 342 is rollingly connected in the two semi-elliptical guide grooves 373. Through the two semi-elliptical guide grooves 373 and the roller 342 in the guide seat 37, while the quick connector 34 rotates, the guide seat 37 can be lifted and lowered by the roller 342 according to the lifting of the semi-elliptical guide groove 373, so as to drive the temperature-controlled stirring paddle 33 to move up and down.

[0054] Reference Figure 9 、 Figure 11 、 Figure 12 、 Figure 13 and Figure 14 As shown, the double-axis rotary joint 35 includes a second fixing nut 351, a circular connecting cylinder 352, a sealing cover 354 and a fixed pressing plate 355. The lower end of the second fixing nut 351 is screwed tightly on the threaded connection head 344 at the top end of the second circular connecting column 341. The inner circle of the upper end of the second fixing nut 351 is telescopically installed on the outer periphery of the lower end of the circular connecting cylinder 352. Four third connection holes 353 are provided at the lower end of the circular connecting cylinder 352. A second positioning groove communicates with the outer periphery of the lower end of the third connection hole 353. Four sealing gaskets 346 at the upper end of the second circular connecting column 341 are respectively positioned and fitted into the four second positioning grooves. An inlet pipe 358 is coaxially and fixedly connected in the circular connecting cylinder 352. The inlet pipe 358 communicates with the two third connection holes 353. The inner cavities of the other two third connection holes 353 and the inner cavity between the inner circle of the circular connecting cylinder 352 and the outer circle of the inlet pipe 358 communicate with each other. The outer circle of the sealing cover 354 is rotatably installed on the periphery of the opening of the circular connecting cylinder 352 through a first bearing. The inner circle of the sealing cover 354 is rotatably installed on the periphery of the inlet pipe 358 through a second bearing. A combined sealing piece is fitted on the inner top surface of the sealing cover 354;

[0055] The outer diameter of the combined seal is the same as the inner diameter of the circular connecting cylinder 352, and the inner diameter is the same as the outer diameter of the inlet pipe 358. The fixed pressing plate 355 is extruded and installed on the upper surface of the sealing cover 354. A first connecting pipe is fixedly installed at the center of the fixed pressing plate 355. The lower end of the first connecting pipe communicates with the inner cavity of the inlet pipe 358. Two second connecting pipes are fixedly connected to both sides of the fixed pressing plate 355. The lower ends of the second connecting pipes hermetically pass through the sealing cover 354 and are threadedly connected with nuts to the combined seal. A second sealing rubber sheet 359 is arranged between the sealing cover 354 and the fixed pressing plate 355. The second sealing rubber sheet 359 is made of rubber. Based on the fact that the outer diameter of the combined seal is the same as the inner diameter of the circular connecting cylinder 352, and the inner diameter of the combined seal is the same as the outer diameter of the inlet pipe 358;

[0056] When the water in the inner cavity between the inner ring of the circular connecting cylinder 352 and the outer ring of the inlet pipe 358 presses the combined seal upward, due to the elastic deformation of the second sealing rubber sheet 359, the greater the extrusion force received by the lower part of the combined seal, the easier the inner and outer ring edges of the two second sealing rubber sheets 359 deform and fit more tightly to fill and seal the outer ring of the inlet pipe 358 and the inner ring of the circular connecting cylinder 352. The combined seal is composed of two stainless steel sheets 357 and two first sealing rubber sheets 356. The two stainless steel sheets 357 and the two first sealing rubber sheets 356 are cross-overlapped and connected. The upper first sealing rubber sheet 356 is clamped and connected between the stainless steel sheet 357 and the sealing cover 354. The lower surface of the lower stainless steel sheet 357 is pressed against the nut.

[0057] Reference Figure 2 and Figure 3 As shown in the reference, a support frame 1 is connected to the lower part of the constant temperature circulator 5 and the tank body 31. A temperature sensor 38 for measuring the internal temperature of the tank body 31 is fixedly installed on the tank cover 32. The constant temperature circulator 5 includes an electric water heater 51, a cold water tank 52, a constant temperature mixing valve 53, a circulation water pump 54, and a flow dividing valve 55. The electric water heater 51 and the cold water tank 52 are fixedly installed on the support frame 1. The constant temperature mixing valve 53 is fixedly installed between the electric water heater 51 and the cold water tank 52. The two water inlets of the constant temperature mixing valve 53 are respectively communicated with the water outlets of the electric water heater 51 and the cold water tank 52. The water outlet of the constant temperature mixing valve 53 is fixedly communicated with the water inlet of the circulation water pump 54. The circulation water pump 54 is fixedly installed above the electric water heater 51. The water outlet of the circulation water pump 54 is fixedly communicated with the water inlet of the flow dividing valve 55. One water outlet of the flow dividing valve 55 is communicated with the first connecting pipe. The other water outlet of the flow dividing valve 55 is connected to the water inlet at the lower end of the temperature control interlayer 311. The water outlet at the upper end of the temperature control interlayer 311 and the water outlets of the two second connecting pipes are all connected to the water inlet of the electric water heater 51. An overflow valve is installed at the upper end of the electric water heater 51. The cold water tank 52 is connected to a cold water source.

[0058] ReferenceFigure 9 and Figure 10 As shown in Figure 10 , at the outer periphery of the top ends of the four first connection holes 333 in the first circular connection column 331, a first positioning groove 337 is communicatively provided. At both ends of the second circular connection column 341, threaded connectors 344 are fixedly installed. Inside the second circular connection column 341, four mutually parallel second connection holes 347 are provided. And at both ends of the second connection holes 347 in the second circular connection column 341, positioning cylinders 345 are fixedly communicated. At the outer end of the positioning cylinder 345, a sealing gasket 346 is fixedly connected. The four second connection holes 347 in the second circular connection column 341 respectively correspond to the four first connection holes 333 in the first circular connection column 331. And at the outer periphery of the top end of the first circular connection column 331, a first fixing nut 332 is telescopically installed. The inner thread of the first fixing nut 332 is installed on the threaded connector 344 at the lower end of the second circular connection column 341.

[0059] Refer to Figure 1 and Figure 15 As shown in Figure 15 , on one side of the support frame 1, an inert gas high-pressure tank 4 is fixedly installed. On the tank cover 32, a pressure sensor for measuring the internal pressure of the tank body 31 is fixedly installed. The tank cover 32 is fixedly communicated with a gas guide pipe. Above the gas guide pipe, a two-position three-way solenoid valve 39 is fixedly installed. The air inlet of the two-position three-way solenoid valve 39 is communicatively connected with the air outlet at the top end of the inert gas high-pressure tank 4. The air outlet of the two-position three-way solenoid valve 39 and the air outlet of the gas guide pipe are fixedly communicated.

[0060] Refer to Figure 1 As shown in Figure 1 , on one side of the support frame 1, a control cabinet 2 is also fixedly installed. The control cabinet 2 is arranged below the constant temperature circulator 5 and is used to control the operation of the entire device;

[0061] The control cabinet 2 is connected to the constant temperature circulator 5 through wires. The control circuit in the control cabinet 2 sends control signals to the electric water heater 51, cold water tank 52, constant temperature mixing valve 53, circulation water pump 54 and flow dividing valve 55 in the constant temperature circulator 5. The temperature sensor 38 monitors the internal temperature of the tank body 31 in real time and feeds back the temperature signal to the control cabinet 2. When the temperature is lower than the set value, the control cabinet 2 controls the electric water heater 51 to heat, and at the same time adjusts the constant temperature mixing valve 53 to make more hot water enter the circulation. When the temperature is higher than the set value, the control cabinet 2 controls the cold water tank 52 to increase the cold water supply, and controls the circulation water flow by adjusting the rotation speed of the circulation water pump 54, thereby accurately regulating the temperature of the culture solution in the tank body 31. Through the control of the constant temperature circulator 5 by the control cabinet 2, the temperature adjustment of the temperature-controlled stirring paddle 33 and the temperature-adjusting interlayer 311 is indirectly realized. The circulating water enters the temperature-controlled stirring paddle 33 through the constant temperature circulator 5, double-axis rotary joint 35, and quick connector 34, and flows into the temperature-adjusting interlayer 311. The control cabinet 2 realizes the coordinated control of the internal temperature of the tank body 31 from the inside to the outside by adjusting the temperature and flow of the circulating water;

[0062] The control cabinet 2 is connected to the two-way three-way solenoid valve 39 by wires. The pressure sensor monitors the internal pressure of the tank body 31 in real time and transmits the pressure signal to the control cabinet 2. When the pressure in the tank body 31 is lower than the set value, the control cabinet 2 sends a signal to open the two-way three-way solenoid valve 39, so that the inert gas in the inert gas high-pressure tank 4 enters the tank body 31 through the gas guide pipe, increasing the air pressure in the tank. When the pressure is higher than the set value, the control cabinet 2 controls the two-way three-way solenoid valve 39 to close, preventing the gas from continuing to enter;

[0063] The control cabinet 2 is connected to the stirring motor 61 by wires. The control cabinet 2 adjusts the current frequency or voltage magnitude output to the stirring motor 61 according to the preset program or the operator's instruction, thereby changing the rotation speed of the stirring motor 61. The change in the rotation speed of the stirring motor 61 affects the rotation speed of the quick connector 34 and the temperature-controlled stirring paddle 33 through the transmission of the speed reducer 62, the spline gear 63 and the lifting gear 343. The stirring speed affects the mixing degree and heat transfer efficiency of the culture solution. Appropriately increasing the rotation speed of the stirring motor 61 can make the culture solution mix more evenly, which is beneficial to the uniform distribution of temperature, thereby assisting in temperature control. At the same time, the stirring process may cause slight fluctuations in the air pressure in the tank body 31. When the control cabinet 2 adjusts the rotation speed of the stirring motor 61, it needs to comprehensively consider the air pressure signal fed back by the pressure sensor to ensure that while adjusting the rotation speed, the air pressure in the tank body 31 is maintained stable.

[0064] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art of this industry should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. An enzyme digestive aid production and manufacturing device, including a biological culture tank (3), the biological culture tank (3) includes a tank body (31) and a tank cover (32), the tank cover (32) is sealed and installed above the opening of the inner cavity of the tank body (31), and is characterized in that: A temperature-controlled stirring paddle (33) is rotatably installed inside the tank body (31). The temperature-controlled stirring paddle (33) includes a first circular connecting column (331), a first U-shaped tube (334), and a second U-shaped tube (335). Four mutually parallel first connecting holes (333) are provided inside the first circular connecting column (331). The outer wall of the first circular connecting column (331) is rotatably installed in a closed manner on the central axis of the tank cover (32). The second U-shaped tube (335) is cross-arranged above the first U-shaped tube (334). The outer width of the first U-shaped tube (334) is greater than the outer width of the second U-shaped tube (335). Two mutually symmetric spiral twist tubes (336) are fixedly connected above both ends of the first U-shaped tube (334) and the second U-shaped tube (335). The tops of the four spiral twist tubes (336) are fixedly connected below the four first connecting holes (333) in the first circular connecting column (331). Moreover, the cross-sections of the first U-shaped tube (334), the second U-shaped tube (335), and the spiral twist tube (336) are all flat. A quick connector (34) and a rotary actuator (6) for driving the quick connector (34) to rotate are coaxially and fixedly installed above the tank body (31). The first circular connecting column (331) is detachably connected to the lower end of the quick connector (34). A two-axis rotary joint (35) is rotatably connected to the top of the quick connector (34). The quick connector (34) is connected to a constant temperature circulator (5) through the two-axis rotary joint (35). The quick connector (34) includes a second circular connecting column (341). A roller (342) is rotatably installed on one side of the second circular connecting column (341). A guide seat (37) is arranged around the quick connector (34) for guiding the quick connector (34) and the connected temperature-controlled stirring paddle (33) to perform reciprocating up and down movements while rotating. The guide seat (37) includes two semi-cylindrical plate blocks (371) that are symmetrically joined together to form the entire cylinder and a fixed seat (372). The second circular connecting column (341) is coaxially rotatably installed between the two semi-cylindrical plate blocks (371). The two semi-cylindrical plate blocks (371) are fixedly installed above the tank cover (32) through the fixed seat (372). Moreover, semi-elliptical guide grooves (373) are provided on the inner walls of the two semi-cylindrical plate blocks (371). The two ends of the two semi-elliptical guide grooves (373) are interconnected. The left end of the semi-elliptical guide groove (373) is higher than the right end. The end of the roller (342) is rollingly connected in the two semi-elliptical guide grooves (373).

2. The enzyme-based digestive aid production and manufacturing device according to claim 1, characterized in that: The double-axis rotary joint (35) includes a second fixing nut (351), a circular connecting cylinder (352), a sealing cover (354) and a fixing pressing plate (355). The inner ring of the upper end of the second fixing nut (351) is telescopically installed on the outer periphery of the lower end of the circular connecting cylinder (352). Four third connecting holes (353) are provided at the lower end of the circular connecting cylinder (352). A second positioning groove is communicated with the outer periphery of the lower end of the third connecting hole (353). Four sealing gaskets (346) at the upper end of the second circular connecting column (341) are respectively positioned and fitted into the four second positioning grooves. An inlet pipe (358) is coaxially and fixedly connected inside the circular connecting cylinder (352). The inlet pipe (358) is communicated with two of the third connecting holes (353). The inner cavities of the other two third connecting holes (353) and the inner cavity between the inner ring of the circular connecting cylinder (352) and the outer ring of the inlet pipe (358) are communicated with each other. The outer ring of the sealing cover (354) is rotatably installed on the periphery of the opening of the circular connecting cylinder (352) through a first bearing. The inner ring of the sealing cover (354) is rotatably installed on the periphery of the inlet pipe (358) through a second bearing. A combined sealing piece is fitted on the inner top surface of the sealing cover (354). The outer diameter of the combined sealing piece is the same as the inner diameter of the circular connecting cylinder (352). The inner diameter of the combined sealing piece is the same as the outer diameter of the inlet pipe (358). The fixing pressing plate (355) is press-fitted on the upper surface of the sealing cover (354). A first connecting pipe is fixedly installed at the center of the fixing pressing plate (355). The lower end of the first connecting pipe is communicated with the inner cavity of the inlet pipe (358). Two second connecting pipes are fixedly connected to both sides of the fixing pressing plate (355). The lower ends of the second connecting pipes are hermetically passed through the sealing cover (354) and are threadedly connected with nuts through the combined sealing piece. A second sealing rubber sheet (359) is provided between the sealing cover (354) and the fixing pressing plate (355).

3. The enzyme-based digestive aid production and manufacturing device according to claim 2, wherein: A support frame (1) is connected below the constant temperature circulator (5) and the tank body (31). A temperature sensor (38) for measuring the temperature inside the tank body (31) is fixedly installed on the tank cover (32). The constant temperature circulator (5) includes an electric water heater (51), a cold water tank (52), a constant temperature mixing valve (53), a circulation water pump (54), and a flow dividing valve (55). The electric water heater (51) and the cold water tank (52) are fixedly installed on the support frame (1). The constant temperature mixing valve (53) is fixedly installed between the electric water heater (51) and the cold water tank (52), and the two water inlets of the constant temperature mixing valve (53) are respectively communicated with the water outlets of the electric water heater (51) and the cold water tank (52). The water outlet of the constant temperature mixing valve (53) is fixedly communicated with the water inlet of the circulation water pump (54). The circulation water pump (54) is fixedly installed above the electric water heater (51). The water outlet of the circulation water pump (54) is fixedly communicated with the water inlet of the flow dividing valve (55). One water outlet of the flow dividing valve (55) is communicated with the first connecting pipe.

4. The enzyme-based digestive aid production and manufacturing device according to claim 1, characterized in that: A first positioning groove (337) is communicated and arranged around the top ends of the four first connecting holes (333) in the first circular connecting column (331). Threaded connectors (344) are fixedly installed at both ends of the second circular connecting column (341). Four mutually parallel second connecting holes (347) are arranged in the second circular connecting column (341), and positioning cylinders (345) are fixedly communicated at both ends of the second connecting holes (347) in the second circular connecting column (341). A sealing gasket (346) is fixedly connected to the outer end of the positioning cylinder (345). The four second connecting holes (347) in the second circular connecting column (341) respectively correspond to the four first connecting holes (333) in the first circular connecting column (331). A first fixing nut (332) is telescopically installed around the top end of the first circular connecting column (331), and the inner circle of the first fixing nut (332) is threadedly installed on the threaded connector (344) at the lower end of the second circular connecting column (341).

5. The enzyme-based digestive aid production and manufacturing device according to claim 2, characterized in that: The combined sealing sheet is composed of two stainless steel sheets (357) and two first sealing rubber sheets (356), and the two stainless steel sheets (357) and the two first sealing rubber sheets (356) are cross-overlapped and adhesively connected. One of the upper first sealing rubber sheets (356) is clamped and connected between the stainless steel sheet (357) and the sealing cover (354), and the lower surface of the lower stainless steel sheet (357) is pressed against the nut.

6. The enzyme-based digestive aid production and manufacturing device according to claim 1, characterized in that: The rotating actuator (6) is composed of a stirring motor (61), a speed reducer (62) and a spline gear (63). The casings of the stirring motor (61) and the speed reducer (62) are fixedly connected to the tank cover (32). The output end of the rotor in the stirring motor (61) is interconnected through the speed reducer (62) and the spline gear (63). The top end of the second circular connecting column (341) is coaxially connected with a lifting gear (343). The outer rings of the lifting gear (343) and the spline gear (63) are meshed with each other, and the outer walls of the lifting gear (343) and the spline gear (63) are slidably connected vertically.

7. The enzyme-based digestive aid production and manufacturing device according to claim 3, characterized in that: A temperature control interlayer (311) is arranged on the outer layer of the tank body (31). One water outlet of the flow dividing valve (55) is connected to the water inlet at the lower end of the temperature control interlayer (311). The water outlet at the upper end of the temperature control interlayer (311) and the water outlets of the two second connecting pipes are all connected to the water inlet of the electric water heater (51).

8. The enzyme-based digestive aid production and manufacturing device according to claim 1, characterized in that: A flow guiding ring (36) is fitted and slidably installed on the inner wall of the tank body (31). The flow guiding ring (36) is arranged on the periphery of the temperature control type stirring paddle (33). The flow guiding ring (36) includes two coaxially arranged rings (361), two arc-shaped connecting plates (362) and a plurality of arc-shaped flow guiding plates (363). The two arc-shaped connecting plates (362) are symmetrically connected to both sides of the two rings (361) respectively. The plurality of arc-shaped flow guiding plates (363) are symmetrically installed on the inner walls of the two arc-shaped connecting plates (362) respectively. The arc-shaped connecting plates (362) are inclined.

9. The enzyme-based digestive aid production and manufacturing device according to claim 3, characterized in that: An inert gas high-pressure tank (4) is fixedly installed on one side of the support frame (1). A pressure sensor for measuring the internal pressure of the tank body (31) is fixedly installed on the tank cover (32). A gas guide pipe is fixedly communicated with the tank cover (32). A two-position three-way electromagnetic valve (39) is fixedly installed above the gas guide pipe. The air inlet of the two-position three-way electromagnetic valve (39) is communicated with the air outlet at the top end of the inert gas high-pressure tank (4). The air outlet of the two-position three-way electromagnetic valve (39) is fixedly communicated with the air outlet of the gas guide pipe.

10. The enzyme-based digestive aid production and manufacturing device according to claim 3, wherein: A control cabinet (2) is also fixedly installed on one side of the support frame (1). The control cabinet (2) is arranged below the constant temperature circulator (5).

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