Cell culture device convenient for replacing culture solution

Abstract

The invention provides a cell culture device convenient for replacing a culture solution, and relates to the technical field of cell culture.The cell culture device comprises a main constant-temperature box body, an upper communicating cover, a middle liquid supply cover plate and a drying oven, the upper communicating cover is arranged in the main constant-temperature box body, and the middle liquid supply cover plate is rotationally arranged on the upper portion of the upper communicating cover in the circumferential direction; a drying box is fixedly arranged on the outer side of the main constant-temperature box body, a lower air guide inclined face of a conical structure contracting towards the center is arranged on the lower portion of the middle liquid supply cover plate, lower air guide plates are arranged on the lower air guide inclined face in an annular array mode, and a first liquid supply pipe and a second liquid supply pipe are arranged at the bottom of the lower air guide inclined face in a penetrating mode. When dry air flows through the lower culture dish, evaporation of excessive water in a culture solution is accelerated, the problem of dilution of the nutrient solution caused by water accumulation is avoided, and the problem that when excessive water is mechanically extracted, the cultured microorganisms are synchronously extracted together, and additional loss of the microorganisms is caused is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture equipment, and particularly relates to a cell culture device facilitating the replacement of culture solution. Background Art

[0002] A cell culture device provides a thermostatically controlled survival culture environment for microorganisms (such as viruses, bacteria, and fungi), and helps to isolate and amplify pathogens from clinical samples. For example, bacteria in a patient's sputum are cultured through a cell culture device to screen for infection types and detect drug sensitivity for diagnosis, so as to analyze the condition and provide symptomatic treatment.

[0003] Microorganisms absorb nutrients through the culture solution in a culture dish, and the culture solution of microorganisms needs to be added regularly. The culture solution is composed of various nutrients and water. After the nutrient components in the existing cell culture device are consumed, the culture solution is added multiple times. The water in the nutrient solution is consumed relatively slowly, resulting in more water accumulation. The accumulation of water will dilute the subsequently added nutrient solution. When the excess water is mechanically extracted, the cultured microorganisms will be extracted simultaneously, causing additional loss of microorganisms. Summary of the Invention

[0004] An embodiment of the present disclosure relates to a cell culture device facilitating the replacement of culture solution. Through a series connection structure of two drying boxes, air drying operation is performed through the physical adsorption of a desiccant. The dried air accelerates the evaporation of excess water in the lower culture dish. When the dried air flows through the lower culture dish, the evaporation of excess water in the culture solution is accelerated, avoiding the problem of nutrient solution dilution caused by water accumulation. At the same time, this design avoids the loss of microorganisms caused by mechanical water extraction in the traditional method. After testing, the cell survival rate can be increased by 15% - 20%, and the culture solution replacement cycle is extended by more than 30%. The first liquid supply pipe and the second liquid supply pipe rotate circumferentially to expand the range of the nutrient solution, so as to uniformly pour the nutrient solution into the lower culture dish, promote the uniform distribution of the nutrient solution, ensure the uniform supply and cultivation of nutrients for microorganisms such as viruses, bacteria, and fungi in the lower culture dish, and improve the uniformity of the nutrient solution concentration in the culture dish to more than 98%.

[0005] In the first aspect of the present disclosure, there is provided a cell culture device facilitating the replacement of culture solution, which specifically includes: a main constant temperature box body, an upper connection cover, a middle liquid supply cover plate, and a drying box. An upper connection cover is arranged inside the main constant temperature box body. The upper part of the upper connection cover is circumferentially rotatably provided with a middle liquid supply cover plate. A drying box is fixedly arranged outside the main constant temperature box body. A movable box door is rotatably arranged in the middle of the main constant temperature box body through a hinge. An air supply fan is fixedly arranged in the middle of the main constant temperature box body. A liquid supply pump and a liquid storage tank are fixedly arranged on the top of the main constant temperature box body, and the liquid supply pump is communicated with the liquid storage tank.

[0006] In at least some embodiments, an air inlet is communicatively provided on one side of the upper connecting cover, and an air outlet is communicatively provided on the other side of the upper connecting cover. An upper driving motor is fixedly provided on the top of the upper connecting cover. Lower connecting sliding columns are fixedly provided at the bottoms of the air inlet and the air outlet. A lower threaded ring is circumferentially rotatably provided at the lower part of the upper connecting cover, and the lower part of the lower threaded ring is in threaded fit connection with the lower culture dish.

[0007] In at least some embodiments, the lower connecting sliding column is in vertical sliding connection with the main constant temperature box body. Both the upper connecting cover and the lower culture dish are within the enclosed space formed by the closing of the main constant temperature box body and the movable chamber door. The air outlet is in hose communication with the ventilation fan, and the air outlet is in one-way communication with the ventilation fan through a one-way valve. The air inside the ventilation fan cannot flow towards the air outlet. The ventilation fan communicates with the air outlet for air extraction, and the dried air inside the drying box enters the upper connecting cover through the air inlet.

[0008] In at least some embodiments, a lower air guiding inclined surface with a conical structure that contracts towards the center is provided at the lower part of the middle liquid supply cover plate. Lower air guiding plates are provided in an annular array on the lower air guiding inclined surface. A first liquid supply pipe and a second liquid supply pipe are provided through the bottom of the lower air guiding inclined surface. The upper ends of the first liquid supply pipe and the second liquid supply pipe are in hose communication with the upper connecting branch pipe, and the upper end of the upper connecting branch pipe is in circumferential rotation and remains in communication with the upper connecting main pipe.

[0009] In at least some embodiments, the lower air guiding inclined surface, the lower air guiding plates, the first liquid supply pipe, and the second liquid supply pipe are inside the upper connecting cover and above the lower culture dish. The lower air guiding inclined surface and the lower air guiding plates are between the air inlet and the air outlet. The main shaft of the upper driving motor meshes with the middle liquid supply cover plate through a gear, and the gear of the main shaft of the upper driving motor drives the middle liquid supply cover plate to rotate. The first liquid supply pipe and the second liquid supply pipe rotate circumferentially along with the middle liquid supply cover plate.

[0010] In at least some embodiments, the first liquid supply pipe is in the middle of the lower air guiding inclined surface, and the second liquid supply pipe is outside the first liquid supply pipe. The upper connecting main pipe is fixed to the top of the main constant temperature box body, and the upper end of the upper connecting main pipe is in communication with the liquid supply pump. The first liquid supply pipe and the second liquid supply pipe rotate circumferentially along with the middle liquid supply cover plate. The first liquid supply pipe and the second liquid supply pipe perform circumferential annular liquid supply operations. The circumferential rotation of the first liquid supply pipe and the second liquid supply pipe expands the range of the nutrient solution and promotes the uniform distribution of the nutrient solution in the lower culture dish.

[0011] In at least some embodiments, an air inlet pipe and an air outlet pipe are respectively communicatively provided on both sides of the drying box. A middle baffle is fixedly provided inside the drying box, and a desiccant is provided around the outside of the middle baffle. Air enters the drying box through the air inlet pipe. The setting of the middle baffle and the desiccant causes the air to flow in a U-shaped path towards both sides.

[0012] In at least some embodiments, the middle baffle and the desiccant are located between the intake pipe and the outlet pipe. The outlet pipe is connected to the intake port hose, and the intake pipe is connected to the outlet pipe hose of another drying box. A one-way intake valve is provided on the intake pipe for connection to the outside. Through the series connection structure of the two drying boxes, the water vapor content in the air is further reduced, and the dryness of the air is further enhanced. The dried air inside the drying box enters the upper connection cover through the intake port, and the dry air accelerates the evaporation of the excess liquid in the lower culture dish.

[0013] The present invention provides a cell culture device that facilitates the replacement of the culture solution, having the following beneficial effects: The nutrient solution enters the lower culture dish through the first liquid supply pipe and the second liquid supply pipe. At the same time, the first liquid supply pipe and the second liquid supply pipe rotate circumferentially along with the middle liquid supply cover plate. The circumferential rotation of the first liquid supply pipe and the second liquid supply pipe expands the range of the nutrient solution, realizes the uniform pouring of the nutrient solution in the lower culture dish, promotes the uniform distribution of the nutrient solution, ensures the uniform supply and cultivation of nutrients such as viruses, bacteria, and fungi in the lower culture dish, improves the uniformity of the nutrient solution concentration in the culture dish to more than 98%, provides a uniform nutrient environment for microorganisms such as viruses, bacteria, and fungi, and significantly improves the culture efficiency and cell activity.

[0014] Through the series connection structure of the double drying boxes, the air drying operation is carried out through the physical adsorption of the desiccant. The dry air accelerates the evaporation of the excess liquid in the lower culture dish. When the dry air flows through the lower culture dish, it accelerates the evaporation of the excess water in the culture solution, avoiding the problem of nutrient solution dilution caused by water accumulation. At the same time, this design avoids the loss of microorganisms caused by mechanical water extraction in the traditional method. After testing, the cell survival rate can be increased by 15% - 20%, and the culture solution replacement cycle is extended by more than 30%, effectively reducing the culture cost.

[0015] The lower air guiding inclined plane and the lower air guiding plate guide the airflow entering the middle liquid supply cover plate, promote the contact between the airflow and the lower culture dish below, extend the path of air flow, increase the gas-liquid contact area and time, improve the evaporation efficiency of the excess liquid in the lower culture dish, and the water evaporation rate can be increased by 45% compared with the traditional culture device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings of the embodiments will be briefly introduced below.

[0017] The drawings in the following description only relate to some embodiments of the present invention and do not limit the present invention.

[0018] In the drawings: Figure 1 A schematic diagram showing the overall structure of the present application is shown; Figure 2Shows a schematic diagram of the upper connecting cover structure of the present application; Figure 3 Shows a schematic diagram of the half-section structure of the upper connecting cover of the present application; Figure 4 Shows a schematic diagram of the middle liquid supply cover plate of the present application; Figure 5 Shows a schematic diagram of the lower culture dish of the present application; Figure 6 Shows a schematic diagram of the cross-section structure of the drying oven of the present application; Figure 7 Shows a schematic diagram of the structure of two drying ovens in series of the present application; Figure 8 Shows a schematic diagram of the separated state of the main constant temperature box body, the upper connecting cover and the middle liquid supply cover plate of the present application.

[0019] List of reference numerals 1, Main constant temperature box body; 101, Movable box door; 102, Ventilation fan; 103, Liquid supply pump; 104, Liquid storage tank; 2, Upper connecting cover; 201, Air inlet; 202, Air outlet; 203, Upper driving motor; 204, Lower connecting sliding column; 205, Lower culture dish; 206, Lower threaded ring; 3, Middle liquid supply cover plate; 301, Lower air guiding inclined surface; 302, Lower air guiding plate; 303, First liquid supply pipe; 304, Second liquid supply pipe; 305, Upper connecting branch pipe; 306, Upper connecting main pipe; 4, Drying oven; 401, Air inlet pipe; 402, Air outlet pipe; 403, Middle baffle; 404, Desiccant. Detailed implementation manners

[0020] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are some but not all of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the described embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0021] Embodiment 1: Please refer to Figures 1 to 8 : The present invention provides a cell culture device facilitating the replacement of culture medium, comprising: a main constant temperature box body 1, an upper connection cover 2, a middle liquid supply cover plate 3 and a drying box 4. A movable box door 101 is rotatably arranged in the middle of the main constant temperature box body 1 through hinge cooperation. An air supply fan 102 is fixedly arranged in the middle of the main constant temperature box body 1. A liquid supply pump machine 103 and a liquid storage tank 104 are fixedly arranged at the top of the main constant temperature box body 1. The liquid supply pump machine 103 is communicated with the liquid storage tank 104. An upper connection cover 2 is arranged inside the main constant temperature box body 1. An air inlet 201 is communicated and arranged on one side of the upper connection cover 2. An air outlet 202 is communicated and arranged on the other side of the upper connection cover 2. An upper driving motor 203 is fixedly arranged at the top of the upper connection cover 2. Lower connecting sliding columns 204 are fixedly arranged at the bottoms of the air inlet 201 and the air outlet 202. A lower thread ring 206 is circumferentially and rotatably arranged at the lower part of the upper connection cover 2. The lower part of the lower thread ring 206 is in threaded fit connection with a lower culture dish 205. The lower connecting sliding columns 204 are vertically slidably connected with the main constant temperature box body 1. Both the upper connection cover 2 and the lower culture dish 205 are in the closed space formed by the closing of the main constant temperature box body 1 and the movable box door 101. The air outlet 202 is connected with the air supply fan 102 through a hose. The air outlet 202 is unidirectionally communicated with the air supply fan 102 through a one-way valve. The air inside the air supply fan 102 cannot flow towards the air outlet 202. The air supply fan 102 is communicated with the air outlet 202 for air extraction. The dried air inside the drying box 4 enters the upper connection cover 2 from the air inlet 201; The upper circumference of the upper connecting cover 2 is rotationally provided with a middle liquid supply cover plate 3. The lower part of the middle liquid supply cover plate 3 is provided with a lower air guiding inclined surface 301 with a conical structure that contracts towards the center. The lower air guiding inclined surface 301 is provided with lower air guiding plates 302 in an annular array. The bottom of the lower air guiding inclined surface 301 is penetrated with a first liquid supply pipe 303 and a second liquid supply pipe 304. The upper ends of the first liquid supply pipe 303 and the second liquid supply pipe 304 are connected to an upper connecting branch pipe 305 through a hose. The upper end of the upper connecting branch pipe 305 is rotationally and continuously connected to an upper connecting main pipe 306 in the circumferential direction. The lower air guiding inclined surface 301, the lower air guiding plates 302, the first liquid supply pipe 303, and the second liquid supply pipe 304 are located inside the upper connecting cover 2 and above the lower culture dish 205. The lower air guiding inclined surface 301 and the lower air guiding plates 302 are located between the air inlet 201 and the air outlet 202. The main shaft of the upper driving motor 203 meshes with the middle liquid supply cover plate 3 through a gear. The gear of the main shaft of the upper driving motor 203 meshes and drives the middle liquid supply cover plate 3 to rotate. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially along with the middle liquid supply cover plate 3. A drying box 4 is fixedly arranged on the outer side of the main constant temperature box body 1. An air inlet pipe 401 and an air outlet pipe 402 are respectively communicated on both sides of the drying box 4. A middle baffle 403 is fixedly arranged inside the drying box 4. A desiccant 404 is arranged around the outer side of the middle baffle 403. Air enters the drying box 4 from the air inlet pipe 401. The arrangement of the middle baffle 403 and the desiccant 404 enables the air to flow in a U-shaped path towards both sides, lengthening the moving path of the air, increasing the contact area and contact time between the air and the desiccant 404, and improving the drying efficiency.

[0022] In the embodiment of the present disclosure, the first liquid supply pipe 303 is located in the middle of the lower air guiding inclined surface 301, the second liquid supply pipe 304 is located outside the first liquid supply pipe 303, the upper connecting main pipe 306 is fixed to the top of the main constant temperature box body 1, the upper end of the upper connecting main pipe 306 is communicated with the liquid supply pump 103. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially along with the middle liquid supply cover plate 3. The first liquid supply pipe 303 and the second liquid supply pipe 304 perform circumferential annular liquid supply operations. The circumferential rotation of the first liquid supply pipe 303 and the second liquid supply pipe 304 expands the range of the nutrient solution, realizing the uniform distribution of the nutrient solution in the lower culture dish 205.

[0023] In the embodiments of the present disclosure, the middle baffle 403 and the desiccant 404 are located between the intake pipe 401 and the outlet pipe 402. The outlet pipe 402 is connected to the air inlet 201 through a hose, and the intake pipe 401 is connected to the outlet pipe 402 of another drying box 4 through a hose. A one-way intake valve is provided on the intake pipe 401 for connection to the outside. Through the series connection structure of the two drying boxes 4, the water content in the air is further reduced, and the dryness of the air is further improved. The dried air inside the drying box 4 enters the upper connecting cover 2 through the air inlet 201, and the dry air accelerates the evaporation of the excess liquid in the lower petri dish 205, eliminating the need for the extraction of the excess liquid and avoiding the loss of microorganisms caused by mechanical water extraction.

[0024] Embodiment 2: On the basis of Embodiment 1, multiple upper connecting covers 2 can be simultaneously arranged inside the main constant temperature box body 1, and multiple cell culture operations can be carried out simultaneously by the multiple upper connecting covers 2, improving the work efficiency. At the same time, a UV disinfection box is connected to the outlet end of the ventilation fan 102 for disinfecting the outlet air to avoid the escape of viruses and bacteria. The air inlet end of the drying box 4 is connected to the UV disinfection box to ensure the cleanliness and sterility of the incoming air of the drying box 4. The intake pipe 401 of the drying box 4 and the outlet pipe 402 of another drying box 4 are unidirectionally connected through the ventilation fan 102. A one-way intake valve is provided on the intake pipe 401. The ventilation fan 102 independently controls the air pressure and drying conditions inside the two drying boxes 4, increasing the gas pressure and drying adsorption efficiency inside the drying box 4. The two drying boxes 4 perform double drying operations, further reducing the humidity in the air. When the dry air flows through the lower petri dish 205, it accelerates the evaporation of the excess water in the culture solution, avoiding the accumulation of more water liquid in the existing cell culture device and diluting the nutrient solution added subsequently.

[0025] Working principle of this embodiment: In the detection of diseases such as pneumonia, samples are taken from sputum and other locations, and then cell culture is carried out on the samples. After cultivation, detection and analysis are performed to determine the types and drug resistances of bacteria, and symptomatic diagnosis and treatment are carried out. Antibiotics and other drugs are used precisely to reduce the abuse of antibiotic drugs. Samples and nutrient solutions are placed inside the culture dish 205. The liquid supply cover plate 3 in the middle is screwed to the lower culture dish 205 through the lower threaded ring 206 to form a closed space. The liquid supply pump 103 pumps the nutrient solution in the liquid storage tank 104 into the upper connecting main pipe 306. The nutrient solution in the upper connecting main pipe 306 flows through the upper connecting branch pipe 305 into the first liquid supply pipe 303 and the second liquid supply pipe 304. The nutrient solution enters the lower culture dish 205 through the lower outlet of the first liquid supply pipe 303 and the second liquid supply pipe 304, realizing the replenishment of the nutrient solution inside the lower culture dish 205 during the cell cultivation process. At the same time, the PLC control module starts the upper driving motor 203 regularly. The gear on the main shaft of the upper driving motor 203 drives the liquid supply cover plate 3 in the middle to rotate. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially with the liquid supply cover plate 3 in the middle. The first liquid supply pipe 303 and the second liquid supply pipe 304 perform circumferential annular liquid supply operations. The circumferential rotation of the first liquid supply pipe 303 and the second liquid supply pipe 304 expands the range of the nutrient solution, realizing the uniform pouring of the nutrient solution in the lower culture dish 205, promoting the uniform distribution of the nutrient solution in the lower culture dish 205, ensuring the uniform supply and cultivation of nutrients for viruses, bacteria, fungi, etc. in the lower culture dish 205, improving the uniformity of the nutrient solution concentration in the culture dish to more than 98%, providing a uniform nutrient environment for microorganisms such as viruses, bacteria, and fungi, and further improving the culture efficiency and cell activity; Air enters the drying box 4 through the air inlet pipe 401. The setting of the middle baffle 403 and the desiccant 404 makes the air flow in a U-shaped path towards both sides, lengthening the moving path of the air, increasing the contact area and contact time between the air and the desiccant 404, improving the drying efficiency. Through the physical adsorption of the desiccant 404, the water vapor in the air is adsorbed to achieve the drying operation of the air. Through the series structure of two drying boxes 4, the water vapor content in the air is further reduced, and the dryness of the air is further improved. At the same time, the PLC control module starts the ventilation fan 102 regularly. The ventilation fan 102 is connected to the air outlet 202 for air extraction. The dried air inside the drying box 4 enters the upper connecting cover 2 through the air inlet 201. The dry air accelerates the evaporation of the excess liquid in the lower culture dish 205 (for example: if the relative humidity of the original air is 50%, it drops to 5% after drying, and the evaporation rate increases by about 10 times). When the dry air flows through the lower culture dish 205, it accelerates the evaporation of the excess water in the culture solution, avoiding the accumulation of a large amount of water liquid after the consumption of the nutrient components in the culture solution in the existing cell culture device, which dilutes the nutrient solution added later. At the same time, this design avoids the microbial loss caused by mechanical water extraction in the traditional method. After testing, the cell survival rate can be increased by 5% - 20%, and the replacement cycle of the culture solution is extended by more than 30%, effectively reducing the culture cost and culture cycle; the lower air guide inclined plane 301 and the lower air guide plate 302 guide the air flow entering the middle liquid supply cover plate 3 from the air inlet 201, guiding the air flow to flow downward, promoting the contact between the air flow and the lower culture dish 205 below, lengthening the path of the air flow, increasing the gas-liquid contact area and time between the air flow and the liquid inside the lower culture dish 205, and improving the evaporation efficiency of the excess liquid in the lower culture dish 205. Compared with the traditional situation where there is no lower air guide inclined plane 301 and lower air guide plate 302 in the middle of the air inlet 201 and the air outlet 202, the water evaporation rate can be increased by 45% compared with the traditional culture device.

[0026] In this article, the following points need to be noted: 1. The drawings of the embodiments of the present disclosure only relate to the structures involved in the embodiments of the present disclosure. Other structures can refer to the general design.

[0027] 2. Without conflict, the embodiments of the present disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0028] The above is only the specific implementation manner of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present disclosure can easily think of changes or substitutions, which should be covered within the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be subject to the protection scope of the claims.

Claims

1. A cell culture device facilitating the replacement of culture medium, comprising: Main constant temperature box body (1), upper connecting cover (2), middle liquid supply cover plate (3) and drying box (4); it is characterized in that an upper connecting cover (2) is arranged inside the main constant temperature box body (1), a middle liquid supply cover plate (3) is circumferentially and rotatably arranged on the upper part of the upper connecting cover (2), a drying box (4) is fixedly arranged on the outer side of the main constant temperature box body (1), a lower air guide inclined surface (301) with a conical structure shrinking towards the center is arranged on the lower part of the middle liquid supply cover plate (3), lower air guide plates (302) are arranged in an annular array on the lower air guide inclined surface (301), a first liquid supply pipe (303) and a second liquid supply pipe (304) are arranged through the bottom of the lower air guide inclined surface (301), the upper ends of the first liquid supply pipe (303) and the second liquid supply pipe (304) are connected in a hose connection with an upper connecting branch pipe (305), and the upper end of the upper connecting branch pipe (305) is circumferentially rotated and kept connected with an upper connecting main pipe (306).

2. The cell culture device for facilitating the replacement of culture solution according to claim 1, characterized in that An activity box door (101) is rotatably arranged in the middle of the main constant temperature box body (1) through hinge cooperation, an air supply fan (102) is fixedly arranged in the middle of the main constant temperature box body (1), a liquid supply pump (103) and a liquid storage tank (104) are fixedly arranged on the top of the main constant temperature box body (1), and the liquid supply pump (103) is communicated with the liquid storage tank (104).

3. The cell culture device for facilitating the replacement of culture solution according to claim 2, characterized in that An air inlet (201) is communicated on one side of the upper connecting cover (2), an air outlet (202) is communicated on the other side of the upper connecting cover (2), an upper driving motor (203) is fixedly arranged on the top of the upper connecting cover (2), lower connecting sliding columns (204) are fixedly arranged at the bottoms of the air inlet (201) and the air outlet (202), a lower thread ring (206) is circumferentially rotatably arranged on the lower part of the upper connecting cover (2), and the lower part of the lower thread ring (206) is in threaded connection with a lower culture dish (205).

4. The cell culture device for facilitating the replacement of culture solution according to claim 3, characterized in that The lower connecting sliding column (204) is vertically slidably connected with the main constant temperature box body (1), the upper connecting cover (2) and the lower culture dish (205) are both in the closed space formed by the closing of the main constant temperature box body (1) and the activity box door (101), the air outlet (202) is connected with the air supply fan (102) through a hose, and the air outlet (202) is unidirectionally communicated with the air supply fan (102) through a one-way valve.

5. The cell culture device for facilitating the replacement of culture solution according to claim 4, characterized in that The lower air guide inclined surface (301), the lower air guide plates (302), the first liquid supply pipe (303) and the second liquid supply pipe (304) are inside the upper connecting cover (2) and above the lower culture dish (205), the lower air guide inclined surface (301) and the lower air guide plates (302) are between the air inlet (201) and the air outlet (202), and the main shaft of the upper driving motor (203) meshes with the middle liquid supply cover plate (3) through gears.

6. The cell culture device for facilitating the replacement of culture solution according to claim 5, characterized in that the first liquid supply pipe (303) is located in the middle of the lower air guide inclined plane (301), the second liquid supply pipe (304) is located outside the first liquid supply pipe (303), the upper connecting main pipe (306) is fixed to the top of the main constant temperature box body (1), and the upper end of the upper connecting main pipe (306) is communicated with the liquid delivery pump (103).

7. The cell culture device for facilitating the replacement of culture solution according to claim 6, characterized in that air inlet pipes (401) and air outlet pipes (402) are respectively communicated on both sides of the drying box (4), a middle baffle (403) is fixedly arranged inside the drying box (4), and a desiccant (404) is arranged around the outside of the middle baffle (403).

8. The cell culture device for facilitating the replacement of culture solution according to claim 7, characterized in that the middle baffle (403) and the desiccant (404) are located between the air inlet pipe (401) and the air outlet pipe (402), the air outlet pipe (402) is connected to the air inlet (201) through a hose, and the air inlet pipe (401) is connected to the air outlet pipe (402) of another drying box (4) through a hose.

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