Compound clostridium butyricum fermentation equipment for aquatic products
By setting up a composite Clostridium butyrate fermentation equipment with slidable piston and through-trough structure at the bottom of the fermentation tank, the problems of bacterial contamination and energy consumption in the multi-stage culture process in the prior art are solved, and seamless transfer and efficient operation of multi-stage culture are achieved.
Patent Information
- Application Number
- CN202510653746.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-21
- Publication Date
- 2025-07-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing fermentation tank volume is fixed, resulting in increased bacterial contamination and energy consumption during multi-stage culture, and it is difficult to achieve seamless transfer of multi-stage culture.
A composite Clostridium butyrate fermentation equipment is designed. By setting a slidable piston and trough structure at the bottom of the fermentation tank, combining a magnetic stirring mechanism and a nitrogen tank, the shape of the fermentation tank bottom is changed, and the first-level small-scale culture, second-level expansion culture and third-level amplified culture are supported to reduce pollution and energy consumption during the transfer process.
The sterile environment creation in the multi-stage culture process is achieved in one-time, avoiding bacterial contamination, reducing energy consumption, and improving operation convenience and the functionality of the fermentor.
Smart Images
Figure CN120366025A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a composite Clostridium butyricum fermentation device, in particular to a composite Clostridium butyricum fermentation device for aquatic products, belonging to the technical field of fermentation devices. Background Art
[0002] The composite Clostridium butyricum for aquatic products is a microecological preparation developed for the aquatic breeding environment. Its core component is Clostridium butyricum (accounting for 50%-70%), and it is compounded with functional strains such as Lactobacillus plantarum and Bacillus subtilis. It is prepared by an anaerobic fermentation process and has the dual effects of intestinal colonization and water quality improvement. Its cells are salt-tolerant (3g - 50g / L NaCl), acid-tolerant (pH 5.0 - 6.5), and bile-salt-resistant. It can quickly colonize in the intestines of aquatic animals, inhibit harmful bacteria such as Vibrio and Aeromonas, repair the intestinal mucosal barrier, and promote nutrient absorption; the short-chain fatty acids such as butyric acid and acetic acid produced by metabolism can regulate the intestinal pH, enhance immunity. At the same time, Bacillus can degrade organic matters such as residual baits and feces in the water body, reduce the contents of ammonia nitrogen and nitrite, and improve the breeding water quality. The product is divided into liquid bacterium agents (the viable bacteria count ≥ 1×10 9 CFU / mL) and solid powder agents (the viable bacteria count ≥ 1×10¹ 0 CFU / g), and is applicable to breeding varieties such as fish, shrimps, crabs, and shellfishes. It can be used by mixing with feed (tolerating high temperature during granulation) or spraying into the water body. It has the advantages of strong colonization ability, wide environmental adaptability, and no risk of drug resistance, and is a key microbial preparation for replacing antibiotics and improving breeding efficiency in green aquatic breeding.
[0003] During the cultivation of the seed liquid, a multi-stage amplification culture system is adopted, and the inoculation amount at each stage is controlled to be the active bacteria in the logarithmic growth phase to ensure that the bacteria can quickly enter the logarithmic growth phase during the next-stage cultivation, shortening the overall cultivation cycle. However, the volume of the current fermentation tank is fixed and can only perform primary cultivation. During the process of multiple transfer fermentation cultivations, it is easy to cause bacterial contamination, resulting in waste of the culture medium, and each time the fermentation tank needs to be sterilized again and an anaerobic environment needs to be created, increasing energy consumption.
[0004] Therefore, a composite Clostridium butyricum fermentation device for aquatic products is designed to optimize the above problems. Summary of the Invention
[0005] The main purpose of the present invention is to provide a composite Clostridium butyricum fermentation device for aquatic products.
[0006] The object of the present invention can be achieved by adopting the following technical solutions: A composite Clostridium butyricum fermentation device for aquatic products, including a fermentation tank. At the middle position of the bottom end of the fermentation tank, a first through groove is vertically opened. A first piston is vertically slidably arranged inside the first through groove. A first upper retaining ring is fixed at the inner top end of the first through groove, and a first lower retaining ring is fixed at the inner bottom end of the first through groove. A second through groove is vertically opened at the middle position of the first piston. A second piston is vertically slidably arranged inside the second through groove. A second upper retaining ring is fixed at the inner top end of the second through groove, and a second lower retaining ring is fixed at the inner bottom end of the second through groove. An electric telescopic rod for controlling the lifting of the second piston is vertically installed at the bottom end of the fermentation tank. A resistance mechanism is arranged between the inner side of the first through groove and the first piston. A nitrogen tank is arranged below the outer side of the fermentation tank. The nitrogen tank is communicated with the inside of the fermentation tank through a trachea. A vacuum pump is installed at the top of the outer side of the fermentation tank. A magnetic stirring mechanism is arranged inside the fermentation tank. A culture medium injection pump is arranged at one side of the top of the fermentation tank away from the vacuum pump. The output end of the culture medium injection pump is communicated with the inside of the fermentation tank. A discharge pipe is arranged at the bottom end of the second piston, and a control valve is arranged on the discharge pipe.
[0007] Preferably: The tops of the first upper retaining ring and the second upper retaining ring are both provided with inclined guide edges, and the inclined guide edges incline towards the middle position of the bottom end of the fermentation tank. The bottom of the first upper retaining ring is completely attached to the top of the first piston, and the bottom of the second upper retaining ring is completely attached to the top of the second piston.
[0008] Preferably: The bottom ends of the fermentation tank are evenly provided with support feet, and anti-slip discs are fixed at the bottom ends of the support feet. Anti-slip grooves are evenly opened at the bottom ends of the anti-slip discs.
[0009] Preferably: A cross plate is arranged at the bottom end of the fermentation tank. The two ends of the cross plate are fixedly connected to the bottom of the fermentation tank. The electric telescopic rod is fixed on the cross plate.
[0010] Preferably: The resistance mechanism includes a chute, an insertion block, a spring and an annular groove. Chutes are evenly opened along the circumference at the top end and the bottom end of the inner side of the first through groove. Insertion blocks are slidably installed inside the chutes. The ends of the insertion blocks protrude inside the first through groove. A spring is arranged between the inner ends of the insertion blocks and the chutes. Annular grooves are opened at the top end and the bottom end of the outer side of the first piston. The end of the insertion block is arc-shaped, and the cross-sectional view of the annular groove is arc-shaped and fits the end face of the insertion block.
[0011] Preferably: A sealing ring is arranged on the outer side of the end of the insertion block away from the spring, and the sealing ring fits the inner side of the chute.
[0012] Preferably, the magnetic stirring mechanism comprises a protective shell, a stirring motor, a mounting plate, a first magnetic ring, a groove, a bearing seat, a stirring shaft, a second magnetic ring, a first stirring rod, a spline groove, a spline shaft, a second stirring rod, a vertical rod and a third stirring rod. The protective shell is fixed at the middle position of the top end of the fermentation tank. A stirring motor is installed at the middle position of the top of the protective shell. A mounting plate is horizontally installed at the output end of the stirring motor. A first magnetic ring is fixed at the bottom of the mounting plate. A groove is formed in the inner top of the fermentation tank. A bearing seat is installed at the bottom of the groove. A stirring shaft is rotatably installed inside the bearing seat. The top end of the stirring shaft extends into the groove. A second magnetic ring is fixed at the top end of the stirring shaft. The first magnetic ring is sleeved outside the second magnetic ring. The first stirring rods are uniformly arranged on the outer side of the stirring shaft. A spline groove is vertically formed at the bottom end of the stirring shaft. A spline shaft is vertically and slidably arranged inside the spline groove. A second stirring rod is installed at the bottom end of the outer side of the spline shaft. A vertical rod is fixed at the bottom end of the spline shaft. A third stirring rod is fixed on the outer side of the vertical rod. The bottom end of the vertical rod is rotatably connected to the top end of the second piston. The length of the third stirring rod is less than the inner diameter of the second upper retaining ring. The length of the second stirring rod is less than the inner diameter of the first upper retaining ring.
[0013] Preferably, the first stirring rod, the second stirring rod and the third stirring rod are all cylindrical in shape, and corrosion-resistant coatings are coated on the outer sides of the first stirring rod, the second stirring rod and the third stirring rod.
[0014] Preferably, an upper guide groove is vertically formed inside the stirring shaft, a lower guide groove is vertically formed inside the spline shaft, the lower guide groove penetrates to the bottom end of the spline shaft, a connecting pipe is arranged between the upper guide groove and the lower guide groove, the length of the vertical rod is less than the depth of the second through groove, a rotary joint is installed at the top end of the stirring shaft, and a conduit is arranged between the top end of the rotary joint and the output end of the culture medium injection pump.
[0015] Preferably, diversion holes are uniformly formed in an annular array at the bottom end of the spline shaft, and the diversion holes are communicated with the bottom end of the lower guide groove.
[0016] The beneficial effects of the present invention are as follows: A composite Clostridium butyricum fermentation device for aquatic products provided by the present invention has a first through groove opened at the bottom of the fermentation tank. A first piston is vertically slidably arranged inside the first through groove. Additionally, a second through groove is opened on the first piston, and a second piston is vertically slidably arranged inside the second through groove, enabling the shape of the bottom of the fermentation tank to be changed during the use of the device. When the first piston is at the top of the first through groove and the second piston is at the bottom of the second through groove, fermentation culture can be carried out inside the second through groove. In this state, primary small-scale culture can be performed; when the first piston is at the bottom of the first through groove and the second piston is at the top of the second through groove, fermentation culture can be carried out inside the second through groove. In this state, secondary expansion culture can be performed; when the first piston is at the top of the first through groove and the second piston is at the top of the second through groove, the fermentation tank can be used for tertiary scale-up culture, improving the functionality of the device, eliminating the need for transfer during fermentation, avoiding bacterial contamination, and only requiring the creation of a sterile environment once, reducing energy consumption and making the operation and use more convenient.
[0017] The magnetic stirring mechanism composed of a protective shell, a stirring motor, a mounting plate, a first magnetic ring, a groove, a bearing seat, a stirring shaft, a second magnetic ring, a first stirring rod, a spline groove, a spline shaft, a second stirring rod, a vertical rod, and a third stirring rod can perform stirring and mixing operations during multi-stage culture, with higher practicality.
[0018] The injection mechanism composed of a culture medium injection pump in cooperation with a conduit, a rotary joint, an upper guide groove, a lower guide groove, and a connecting pipe enables the device to directly inject from the middle position at the bottom of the fermentation tank, without causing waste of raw materials during multi-stage fermentation, and the raw materials will not remain in other positions except the fermentation position, while ensuring the precise configuration of the liquid during multi-stage fermentation. Description of the Drawings
[0019] Figure 1 It is the front view cross-sectional view of a preferred embodiment of a composite Clostridium butyricum fermentation device for aquatic products of the present invention; Figure 2 It is the front view of a preferred embodiment of a composite Clostridium butyricum fermentation device for aquatic products of the present invention; Figure 3 It is the fermentation tank primary culture state diagram of a preferred embodiment of a composite Clostridium butyricum fermentation device for aquatic products of the present invention; Figure 4 It is the fermentation tank secondary culture state diagram of a preferred embodiment of a composite Clostridium butyricum fermentation device for aquatic products of the present invention; Figure 5 It is the fermentation tank tertiary culture state diagram of a preferred embodiment of a composite Clostridium butyricum fermentation device for aquatic products of the present invention; Figure 6A preferred embodiment of a compound Clostridium butyricum fermentation device for aquatic products in the present invention Figure 1 Enlarged view of part A in Figure 7 Structural diagram of the stirring shaft of a preferred embodiment of a compound Clostridium butyricum fermentation device for aquatic products in the present invention Figure 8 A preferred embodiment of a compound Clostridium butyricum fermentation device for aquatic products in the present invention Figure 3 Enlarged view of part B in
[0020] In the figure: 1, fermentation tank; 2, first through groove; 3, first piston; 4, first upper retaining ring; 5, first lower retaining ring; 6, second through groove; 7, second piston; 8, second upper retaining ring; 9, second lower retaining ring; 10, electric telescopic rod 11, resistance mechanism; 1101, chute; 1102, insertion block; 1103, spring; 1104, annular groove 12, nitrogen tank; 13, vacuum pump 14, magnetic stirring mechanism; 1401, protective shell; 1402, stirring motor; 1403, mounting plate; 1404, first magnetic ring; 1405, groove; 1406, bearing seat; 1407, stirring shaft; 1408, second magnetic ring; 1409, first stirring rod; 1410, spline groove; 1411, spline shaft; 1412, second stirring rod; 1413, vertical rod; 1414, third stirring rod 15, culture medium injection pump; 1501, conduit; 1502, rotary joint; 1503, upper guide groove; 1504, lower guide groove; 1506, connecting pipe Detailed implementation method
[0021] To make the technical solutions of the present invention clearer and more definite for those skilled in the art, the present invention will be further described in detail below in conjunction with embodiments and accompanying drawings, but the implementation manners of the present invention are not limited thereto.
[0022] As Figures 1-8As shown in the figure, this embodiment provides a composite Clostridium butyricum fermentation device for aquatic products, including a fermentation tank 1. A first through groove 2 is vertically opened at the middle position of the bottom end of the fermentation tank 1. A first piston 3 is vertically slidably arranged inside the first through groove 2. A first upper retaining ring 4 is fixed at the inner top end of the first through groove 2, and a first lower retaining ring 5 is fixed at the inner bottom end of the first through groove 2. A second through groove 6 is vertically opened at the middle position of the first piston 3. A second piston 7 is vertically slidably arranged inside the second through groove 6. Double O-ring seals are arranged at the edges of the first piston 3 and the second piston 7 to ensure that the friction coefficient between the piston and the inner wall of the through groove during piston movement is ≤0.3, and at the same time prevent external air from infiltrating. A second upper retaining ring 8 is fixed at the inner top end of the second through groove 6, and a second lower retaining ring 9 is fixed at the inner bottom end of the second through groove 6. An electric telescopic rod 10 for controlling the lifting of the second piston 7 is vertically installed at the bottom end of the fermentation tank 1. A resistance mechanism 11 is arranged between the inner side of the first through groove 2 and the first piston 3. A nitrogen tank 12 is arranged below the outside of the fermentation tank 1. The nitrogen tank 12 is communicated with the inside of the fermentation tank 1 through a gas pipe. A vacuum pump 13 is installed at the top of the outside of the fermentation tank 1. A magnetic stirring mechanism 14 is arranged inside the fermentation tank 1. A culture medium injection pump 15 is arranged on one side of the top of the fermentation tank 1 away from the vacuum pump 13. The output end of the culture medium injection pump 15 is communicated with the inside of the fermentation tank 1. A discharge pipe is arranged at the bottom end of the second piston 7, and a control valve is arranged on the discharge pipe. The discharge pipe extends downward from the center of the bottom end of the second piston 7, with an inclination angle ≥15°, a pipe diameter of φ15mm, and an inner wall roughness Ra ≤0.8μm to ensure that the liquid flows by itself without residue. The control valve adopts a sterile diaphragm valve, and the sealing pressure is ≥0.5MPa when closed.
[0023] General working principle: Before use, first sterilize the inside of the fermentation tank 1, and then use the vacuum pump 13 to extract the oxygen inside the fermentation tank 1, reduce the pressure inside the fermentation tank 1 to ≤10 Pa, maintain it for 10 min, remove the air, and then inject sterile nitrogen into the inside of the fermentation tank 1 to create a sterile environment and maintain the tank pressure at 0.02 Mpa. Repeat the vacuum pumping-nitrogen filling cycle 3 times to ensure that the oxygen content is ≤0.1%. When in use, in the initial state, the first piston 3 and the second piston 7 are respectively located at the top of the first through groove 2 and the second through groove 6. When performing a small amount of cultivation, start the electric telescopic rod 10 to control the second piston 7 to move down to the bottom of the second through groove 6, and then inject the culture medium and the activated bacterial liquid into the second through groove 6, and then perform primary cultivation. After the primary seed liquid cultivation is completed, if secondary expansion cultivation is required, start the electric telescopic rod 10 again to control the first piston 3 to move down to the bottom of the first through groove 2, and after the first piston 3 has moved down, control the electric telescopic rod 10 to extend a certain distance. At this time, the first piston 3 is limited by the resistance mechanism 11, and only the second piston 7 moves up until the second piston 7 moves to the top of the second through groove 6, and the primary culture enters the second through groove 6. Then, inject the culture medium into the second through groove 6 for expansion cultivation. When the expanded seed liquid cultivation is completed and tertiary scale-up cultivation is required, at this time, start the electric telescopic rod 10 again to control the first piston 3 to move to the top of the first through groove 2. At this time, the secondary culture enters the bottom of the fermentation tank 1, and the culture medium is injected again for seed liquid scale-up cultivation. During the tertiary cultivation process, the cultivation environment remains unchanged. In addition, during cultivation, the magnetic stirring mechanism 14 is used for stirring during hierarchical cultivation.
[0024] In this embodiment, inclined guide edges are provided at the tops of the first upper retaining ring 4 and the second upper retaining ring 8, and the inclined guide edges are inclined towards the middle position at the bottom end of the fermentation tank 1. The bottom of the first upper retaining ring 4 is completely attached to the top of the first piston 3, and the bottom of the second upper retaining ring 8 is completely attached to the top of the second piston 7.
[0025] Local working principle: The inclined guide grooves provided on the first upper retaining ring 4 and the second upper retaining ring 8 can collect the seed liquid towards the middle of the fermentation tank 1, and after the piston is attached to the retaining ring, no gap will be left, ensuring that the seed liquid can be completely discharged.
[0026] In this embodiment, support feet are uniformly arranged at the bottom end of the fermentation tank 1, and anti-slip discs are fixed to the bottom ends of the support feet. Anti-slip grooves are uniformly formed at the bottom ends of the anti-slip discs. 4 support feet are uniformly arranged at the bottom end of the fermentation tank 1, with a height of 50 - 100 mm, made of 304 stainless steel. The diameter of the anti-slip disc is 100 - 150 mm, the thickness is 5 - 10 mm, the depth of the anti-slip grooves on the bottom surface is 2 - 3 mm, and the spacing is 5 mm, distributed in a grid pattern, ensuring that the static friction coefficient of the equipment on the horizontal ground is ≥0.6.
[0027] Local working principle: During the use of the device, the anti-slip disc is provided to increase the contact area between the bottom of the fermentation tank 1 and the ground, and the anti-slip pattern is opened to ensure the support stability of the fermentation tank 1.
[0028] In this embodiment, a cross plate is provided at the bottom end of the fermentation tank 1, and both ends of the cross plate are fixedly connected to the bottom of the fermentation tank 1, and the electric telescopic rod 10 is fixed on the cross plate.
[0029] Local working principle: The electric telescopic rod 10 is vertically arranged at the bottom of the fermentation tank 1. When the electric telescopic rod 10 is used, it can ensure the vertical movement of the piston.
[0030] In this embodiment, the resistance mechanism 11 includes a chute 1101, a plug 1102, a spring 1103 and an annular groove 1104. Chutes 1101 are uniformly opened along the circumference at the top and bottom inside the first through groove 2. Plugs 1102 are slidably installed inside the chutes 1101. The ends of the plugs 1102 protrude inside the first through groove 2. A spring 1103 is provided between the end of the plug 1102 and the inner end of the chute 1101. Annular grooves 1104 are opened at the top and bottom outside the first piston 3. The end of the plug 1102 is arc-shaped. The cross-sectional view of the annular groove 1104 is arc-shaped and fits the end face of the plug 1102. The arc radius of the end of the plug 1102 is 2 - 5 mm, the depth of the annular groove 1104 is 3 - 6 mm, and the width matches the protruding part of the plug 1102 (tolerance ±0.1 mm). When the plug 1102 is completely embedded in the annular groove 1104, the gap between the first piston 3 and the inner wall of the first through groove 2 ≤ 0.05 mm to avoid liquid leakage. The elastic coefficient k of the spring 1103 = 5 - 10 N / mm, and the pre-compression amount is 5 mm. Ensure that the plug 1102 can be disengaged from the annular groove 1104 under the friction force (0.5 - 1 N) during piston movement, and at the same time provide sufficient holding force (≥2 N) during limiting to prevent the piston from moving automatically due to liquid pressure fluctuations.
[0031] Local working principle: When the first piston 3 moves to the top and bottom of the first through groove 2, the plug 1102 will be squeezed into the annular groove 1104 under the elastic force of the spring 1103, playing a certain limiting role on the first piston 3 to increase the stability of the first piston 3, so as to avoid the first piston 3 being driven to move simultaneously when the second piston 7 slides, making the multi-stage culture adjustment of the seed liquid more convenient.
[0032] In this embodiment, a sealing ring is provided on the outer side of the end of the plug 1102 away from the spring 1103, and the sealing ring fits the inner side of the chute 1101.
[0033] Local working principle: During the expansion culture, due to the blocking effect of the sealing ring, the seed liquid will not enter the inside of the chute 1101.
[0034] In this embodiment, the magnetic stirring mechanism 14 includes a protective shell 1401, a stirring motor 1402, a mounting plate 1403, a first magnetic ring 1404, a groove 1405, a bearing seat 1406, a stirring shaft 1407, a second magnetic ring 1408, a first stirring rod 1409, a spline groove 1410, a spline shaft 1411, a second stirring rod 1412, a vertical rod 1413 and a third stirring rod 1414. The protective shell 1401 is fixed at the middle position of the top of the fermentation tank 1. A stirring motor 1402 is installed at the middle position of the top of the protective shell 1401. The output end of the stirring motor 1402 is horizontally installed with a mounting plate 1403. The bottom of the mounting plate 1403 is fixed with a first magnetic ring 1404. A groove 1405 is opened at the inner top of the fermentation tank 1. A bearing seat 1406 is installed at the bottom of the groove 1405. A stirring shaft 1407 is rotatably installed inside the bearing seat 1406. The top end of the stirring shaft 1407 extends into the groove 1405. A second magnetic ring 1408 is fixed at the top end of the stirring shaft 1407. The first magnetic ring 1404 is sleeved outside the second magnetic ring 1408. The outer side of the stirring shaft 1407 is evenly provided with first stirring rods 1409. A spline groove 1410 is vertically opened at the bottom end of the stirring shaft 1407. A spline shaft 1411 is vertically slidably arranged inside the spline groove 1410. A second stirring rod 1412 is installed at the bottom end of the outer side of the spline shaft 1411. A vertical rod 1413 is fixed at the bottom end of the spline shaft 1411. A third stirring rod 1414 is fixed on the outer side of the vertical rod 1413. The bottom end of the vertical rod 1413 is rotatably connected to the top end of the second piston 7. The length of the third stirring rod 1414 is less than the inner diameter of the second upper retaining ring 8. The length of the second stirring rod 1412 is less than the inner diameter of the first upper retaining ring 4.
[0035] Partial working principle: During the fermentation process, the third stirring rod 1414 at the bottom of the vertical rod 1413 is located inside the second through groove 6. By starting the stirring motor 1402, the stirring shaft 1407, the spline shaft 1411 and the vertical rod 1413 can be driven to rotate, and then the third stirring rod 1414 is used for stirring during the primary fermentation process. The stirring motor 1402 rotates at a speed of 50-100 rpm to ensure that the bacterial liquid is evenly mixed and to avoid shear force damaging the bacterial body. During the secondary expansion stirring process, the second stirring rod 1412 at the bottom of the spline shaft 1411 and the third stirring rod 1414 at the bottom of the vertical rod 1413 are both located inside the first through groove 2. The starting of the stirring motor 1402 can simultaneously control the second stirring rod 1412 and the third stirring rod 1414 to stir the bacteria. The first piston 3 moves downward to expand the space to achieve sufficient mixing of the culture medium and the primary seed liquid. During the process of amplification and cultivation, the first stirring rod 1409, the second stirring rod 1412 and the third stirring rod 1414 are all located inside the fermentation tank 1. The stirring motor 1402 can drive the first stirring rod 1409, the second stirring rod 1412 and the third stirring rod 1414 to rotate and stir at the same time, with a speed of 150-200rpm. The stirring shaft 1407 is fixed by the bearing seat 1406, and the spline shaft 1411 and the spline groove 1410 inside the stirring shaft 1407 have a clearance of ≤0.2mm to prevent liquid from penetrating into the spline groove 1410, and the top of the spline groove 1410 is sealed by a sealing ring.
[0036] In this embodiment, the first stirring rod 1409 , the second stirring rod 1412 , and the third stirring rod 1414 are all cylindrical in shape, and the outer sides of the first stirring rod 1409 , the second stirring rod 1412 , and the third stirring rod 1414 are all coated with a corrosion-resistant coating.
[0037] Partial working principle: During the rotating stirring process, the cylindrical stirring rod has low shear to avoid damage to the bacteria, and the corrosion-resistant coating increases the service life of the stirring rod.
[0038] In this embodiment, an upper guide groove 1503 is vertically formed inside the stirring shaft 1407, a lower guide groove 1504 is vertically formed inside the spline shaft 1411, the lower guide groove 1504 penetrates to the bottom end of the spline shaft 1411, a connecting pipe 1506 is provided between the upper guide groove 1503 and the lower guide groove 1504, the length of the vertical rod 1413 is less than the depth of the second through groove 6, a rotary joint 1502 is installed at the top end of the stirring shaft 1407, and a conduit 1501 is provided between the top end of the rotary joint 1502 and the output end of the culture medium injection pump 15. The diameter of the upper guide groove 1503 is φ10mm, the diameter of the lower guide groove 1504 is φ8mm, and they are communicated through a connecting pipe 1506 with an inner diameter of φ6mm. The flow rate of the culture medium injection pump is adjusted according to the culture volume (Level 1: 50 mL / min, Level 2: 200 mL / min, Level 3: 500 mL / min) to ensure stable liquid pressure (0.1 - 0.3 MPa) during the injection process and avoid the generation of bubbles.
[0039] Local working principle: When the seed liquid and the culture medium liquid are injected, the culture medium injection pump 15 extracts them, then enters the inner part of the upper guide groove 1503 through the conduit 1501 and the rotary joint 1502, then enters the inner part of the lower guide groove 1504 through the connecting pipe 1506, and finally discharges from the inner part of the spline shaft 1411. At the first-stage fermentation, the bottom end of the spline shaft 1411 is inserted into the inner part of the second through groove 6. At the second-stage fermentation, the spline shaft 1411 is also located in the inner part of the first through groove 2. Therefore, it can prevent the seed liquid and the culture medium from scattering to other positions and ensure the accurate configuration of the liquid during the multi-stage fermentation process.
[0040] In this embodiment, diversion holes are evenly formed in a circular array at the bottom end of the spline shaft 1411, and the diversion holes are communicated with the bottom end of the lower guide groove 1504. 6 - 8 diversion holes are formed, with a hole diameter of φ1 - 2mm, distributed obliquely downward at 45°, and a hole pitch of 5 - 10mm, ensuring that the culture medium is evenly sprayed out at a flow rate of 0.5 - 1 m / s to cover the bottom surface area of the second through groove 6 or the first through groove 2.
[0041] Local working principle: Through the formation of multiple diversion holes, the liquid can be evenly diverted and discharged outward, and at the same time, it can play a certain pre-mixing effect.
[0042] The above is only a further embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the scope disclosed by the present invention, according to the technical solution and its concept of the present invention, makes equivalent substitutions or changes, all belong to the protection scope of the present invention.
Claims
1. A composite Clostridium butyricum fermentation device for aquatic products, comprising a fermentation tank (1), characterized in that: At the middle position of the bottom end of the fermentation tank (1), a first through groove (2) is vertically opened. A first piston (3) is vertically slidably arranged inside the first through groove (2). A first upper retaining ring (4) is fixed to the inner top end of the first through groove (2), and a first lower retaining ring (5) is fixed to the inner bottom end of the first through groove (2). A second through groove (6) is vertically opened at the middle position of the first piston (3). A second piston (7) is vertically slidably arranged inside the second through groove (6). A second upper retaining ring (8) is fixed to the inner top end of the second through groove (6), and a second lower retaining ring (9) is fixed to the inner bottom end of the second through groove (6). An electric telescopic rod (10) for controlling the lifting of the second piston (7) is vertically installed at the bottom end of the fermentation tank (1). A resistance mechanism (11) is arranged between the inner side of the first through groove (2) and the first piston (3). A nitrogen gas tank (12) is arranged below the outer side of the fermentation tank (1). The nitrogen gas tank (12) is communicated with the inside of the fermentation tank (1) through a gas pipe. A vacuum pump (13) is installed at the top of the outer side of the fermentation tank (1). A magnetic stirring mechanism (14) is arranged inside the fermentation tank (1). A culture medium injection pump (15) is arranged on one side of the top of the fermentation tank (1) away from the vacuum pump (13). The output end of the culture medium injection pump (15) is communicated with the inside of the fermentation tank (1). A discharge pipe is arranged at the bottom end of the second piston (7), and a control valve is arranged on the discharge pipe.
2. The composite Clostridium butyricum fermentation equipment for aquatic products according to claim 1, characterized in that: Bevel edges are provided at the tops of the first upper retaining ring (4) and the second upper retaining ring (8), and the bevel edges are inclined towards the middle position of the bottom end of the fermentation tank (1). The bottom of the first upper retaining ring (4) is completely attached to the top of the first piston (3), and the bottom of the second upper retaining ring (8) is completely attached to the top of the second piston (7).
3. A composite Clostridium butyricum fermentation device for aquatic products according to claim 1, characterized in that: Support feet are evenly arranged at the bottom end of the fermentation tank (1), and anti-slip discs are fixed to the bottom ends of the support feet. Anti-slip patterns are evenly opened at the bottom ends of the anti-slip discs.
4. A composite Clostridium butyricum fermentation device for aquatic products according to claim 1, characterized in that: A cross plate is arranged at the bottom end of the fermentation tank (1). The two ends of the cross plate are fixedly connected to the bottom of the fermentation tank (1). The electric telescopic rod (10) is fixed on the cross plate.
5. A composite Clostridium butyricum fermentation device for aquatic products according to claim 1, characterized in that: The resistance mechanism (11) includes a chute (1101), an insertion block (1102), a spring (1103), and an annular groove (1104). Chutes (1101) are evenly opened along the circumferential direction at the top and bottom ends of the inner side of the first through groove (2). Insertion blocks (1102) are slidably installed inside the chutes (1101). The ends of the insertion blocks (1102) protrude inside the first through groove (2). A spring (1103) is arranged between the inner ends of the insertion blocks (1102) and the chutes (1101). Annular grooves (1104) are opened at the top and bottom ends of the outer side of the first piston (3). The ends of the insertion blocks (1102) are arc-shaped, and the cross-sectional view of the annular groove (1104) is arc-shaped and fits the end face of the insertion block (1102).
6. The composite Clostridium butyricum fermentation equipment for aquatic products according to claim 5, characterized in that: A sealing ring is arranged on the outer side of the end of the insertion block (1102) away from the spring (1103), and the sealing ring is attached to the inner side of the chute (1101).
7. A composite Clostridium butyricum fermentation device for aquatic products according to claim 1, characterized in that: The magnetic stirring mechanism (14) includes a protective shell (1401), a stirring motor (1402), a mounting plate (1403), a first magnetic ring (1404), a groove (1405), a bearing seat (1406), a stirring shaft (1407), a second magnetic ring (1408), a first stirring rod (1409), a spline groove (1410), a spline shaft (1411), a second stirring rod (1412), a vertical rod (1413) and a third stirring rod (1414). The protective shell (1401) is fixed at the middle position of the top end of the fermentation tank (1). A stirring motor (1402) is installed at the middle position of the top of the protective shell (1401). The output end of the stirring motor (1402) is horizontally installed with a mounting plate (1403). The bottom of the mounting plate (1403) is fixed with a first magnetic ring (1404). A groove (1405) is opened at the inner top of the fermentation tank (1). A bearing seat (1406) is installed at the bottom of the groove (1405). A stirring shaft (1407) is rotatably installed inside the bearing seat (1406). The top end of the stirring shaft (1407) extends into the groove (1405). A second magnetic ring (1408) is fixed at the top end of the stirring shaft (1407). The first magnetic ring (1404) is sleeved outside the second magnetic ring (1408). The outer side of the stirring shaft (1407) is evenly provided with first stirring rods (1409). A spline groove (1410) is vertically opened at the bottom end of the stirring shaft (1407). A spline shaft (1411) is vertically and slidably arranged inside the spline groove (1410). A second stirring rod (1412) is installed at the bottom end of the outer side of the spline shaft (1411). A vertical rod (1413) is fixed at the bottom end of the spline shaft (1411). A third stirring rod (1414) is fixed on the outer side of the vertical rod (1413). The bottom end of the vertical rod (1413) is rotatably connected to the top end of the second piston (7). The length of the third stirring rod (1414) is less than the inner diameter of the second upper retaining ring (8). The length of the second stirring rod (1412) is less than the inner diameter of the first upper retaining ring (4).
8. A composite Clostridium butyricum fermentation device for aquatic products according to claim 7, characterized in that: The shapes of the first stirring rod (1409), the second stirring rod (1412) and the third stirring rod (1414) are all cylindrical, and the outer sides of the first stirring rod (1409), the second stirring rod (1412) and the third stirring rod (1414) are all coated with a corrosion-resistant coating.
9. The composite Clostridium butyricum fermentation equipment for aquatic products according to claim 7, characterized in that: An upper guide groove (1503) is vertically opened inside the stirring shaft (1407). A lower guide groove (1504) is vertically opened inside the spline shaft (1411). The lower guide groove (1504) penetrates to the bottom end of the spline shaft (1411). A connecting pipe (1506) is provided between the upper guide groove (1503) and the lower guide groove (1504). The length of the vertical rod (1413) is less than the depth of the second through groove (6). A rotary joint (1502) is installed at the top end of the stirring shaft (1407). A conduit (1501) is provided between the top end of the rotary joint (1502) and the output end of the culture medium injection pump (15).
10. A composite Clostridium butyricum fermentation device for aquatic products according to claim 9, characterized in that: The bottom end of the spline shaft (1411) is evenly provided with diversion holes in an annular array, and the diversion holes are communicated with the bottom end of the lower guide groove (1504).